Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57572.t1 | XP_024637947.1 | 96.9 | 422 | 10 | 2 | 1 | 419 | 1 | 422 | 1.10E-225 | 792.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57572.t1 | Q6VBB2 | 48.6 | 243 | 115 | 3 | 68 | 303 | 22 | 261 | 4.7e-57 | 223.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57572.t1 | A0A396IIY0 | 96.9 | 422 | 10 | 2 | 1 | 419 | 1 | 422 | 7.7e-226 | 792.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene57572.t1 | TF | HSF |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049368 | MS.gene57572 | 0.809024 | 2.32E-50 | -1.69E-46 |
| MS.gene050361 | MS.gene57572 | 0.803028 | 4.24E-49 | -1.69E-46 |
| MS.gene050384 | MS.gene57572 | 0.801124 | 1.04E-48 | -1.69E-46 |
| MS.gene050386 | MS.gene57572 | 0.803035 | 4.22E-49 | -1.69E-46 |
| MS.gene050414 | MS.gene57572 | 0.823429 | 1.39E-53 | -1.69E-46 |
| MS.gene050646 | MS.gene57572 | 0.803516 | 3.36E-49 | -1.69E-46 |
| MS.gene050654 | MS.gene57572 | 0.82423 | 9.03E-54 | -1.69E-46 |
| MS.gene051856 | MS.gene57572 | 0.981825 | 1.53E-153 | -1.69E-46 |
| MS.gene053931 | MS.gene57572 | 0.807921 | 3.99E-50 | -1.69E-46 |
| MS.gene056045 | MS.gene57572 | 0.828701 | 7.77E-55 | -1.69E-46 |
| MS.gene056181 | MS.gene57572 | 0.829795 | 4.22E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57572.t1 | MTR_3g104550 | 41.226 | 359 | 167 | 9 | 85 | 417 | 33 | 373 | 4.36e-77 | 244 |
| MS.gene57572.t1 | MTR_7g095680 | 47.273 | 275 | 124 | 4 | 66 | 319 | 7 | 281 | 2.90e-75 | 239 |
| MS.gene57572.t1 | MTR_1g102860 | 47.619 | 252 | 115 | 6 | 62 | 303 | 6 | 250 | 2.96e-70 | 225 |
| MS.gene57572.t1 | MTR_5g029680 | 51.707 | 205 | 83 | 3 | 85 | 275 | 21 | 223 | 4.81e-65 | 216 |
| MS.gene57572.t1 | MTR_1g071360 | 47.727 | 220 | 106 | 3 | 85 | 302 | 19 | 231 | 2.70e-63 | 207 |
| MS.gene57572.t1 | MTR_1g071360 | 47.727 | 220 | 106 | 3 | 85 | 302 | 16 | 228 | 5.91e-63 | 207 |
| MS.gene57572.t1 | MTR_4g077970 | 51.852 | 216 | 82 | 5 | 97 | 296 | 11 | 220 | 8.69e-62 | 207 |
| MS.gene57572.t1 | MTR_1g061170 | 49.500 | 200 | 96 | 1 | 85 | 279 | 36 | 235 | 1.29e-61 | 206 |
| MS.gene57572.t1 | MTR_8g087540 | 44.444 | 198 | 100 | 2 | 97 | 284 | 11 | 208 | 2.03e-51 | 178 |
| MS.gene57572.t1 | MTR_2g100680 | 44.041 | 193 | 105 | 1 | 96 | 285 | 29 | 221 | 1.80e-50 | 177 |
| MS.gene57572.t1 | MTR_6g043060 | 41.004 | 239 | 118 | 5 | 98 | 333 | 22 | 240 | 1.37e-48 | 168 |
| MS.gene57572.t1 | MTR_2g100670 | 41.038 | 212 | 113 | 4 | 92 | 299 | 27 | 230 | 1.27e-46 | 162 |
| MS.gene57572.t1 | MTR_2g100670 | 41.038 | 212 | 113 | 4 | 92 | 299 | 135 | 338 | 9.38e-46 | 163 |
| MS.gene57572.t1 | MTR_8g083100 | 45.604 | 182 | 95 | 2 | 99 | 277 | 23 | 203 | 1.62e-45 | 164 |
| MS.gene57572.t1 | MTR_8g105780 | 47.644 | 191 | 86 | 3 | 98 | 274 | 22 | 212 | 3.02e-45 | 160 |
| MS.gene57572.t1 | MTR_6g086805 | 45.263 | 190 | 99 | 2 | 98 | 283 | 11 | 199 | 2.37e-44 | 157 |
| MS.gene57572.t1 | MTR_2g017890 | 43.784 | 185 | 94 | 3 | 97 | 271 | 14 | 198 | 1.86e-41 | 151 |
| MS.gene57572.t1 | MTR_3g101870 | 60.000 | 110 | 43 | 1 | 92 | 201 | 18 | 126 | 1.02e-38 | 143 |
| MS.gene57572.t1 | MTR_5g010680 | 63.441 | 93 | 34 | 0 | 96 | 188 | 22 | 114 | 8.28e-38 | 140 |
| MS.gene57572.t1 | MTR_5g017470 | 62.766 | 94 | 35 | 0 | 95 | 188 | 6 | 99 | 1.15e-37 | 138 |
| MS.gene57572.t1 | MTR_5g089170 | 64.516 | 93 | 33 | 0 | 96 | 188 | 23 | 115 | 7.49e-37 | 135 |
| MS.gene57572.t1 | MTR_7g091370 | 53.571 | 112 | 48 | 1 | 89 | 196 | 9 | 120 | 5.20e-36 | 132 |
| MS.gene57572.t1 | MTR_1g100777 | 58.065 | 93 | 39 | 0 | 96 | 188 | 16 | 108 | 8.93e-34 | 128 |
| MS.gene57572.t1 | MTR_0020s0020 | 59.140 | 93 | 38 | 0 | 96 | 188 | 31 | 123 | 5.16e-33 | 127 |
| MS.gene57572.t1 | MTR_4g088900 | 56.701 | 97 | 38 | 1 | 96 | 188 | 31 | 127 | 1.30e-31 | 120 |
| MS.gene57572.t1 | MTR_1g061170 | 41.732 | 127 | 69 | 1 | 158 | 279 | 14 | 140 | 1.06e-23 | 102 |
| MS.gene57572.t1 | MTR_4g045757 | 58.209 | 67 | 28 | 0 | 89 | 155 | 5 | 71 | 5.93e-20 | 84.0 |
| MS.gene57572.t1 | MTR_5g047190 | 46.316 | 95 | 44 | 2 | 106 | 193 | 1 | 95 | 2.43e-19 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57572.t1 | AT2G26150 | 40.950 | 337 | 144 | 8 | 85 | 407 | 31 | 326 | 5.01e-76 | 240 |
| MS.gene57572.t1 | AT2G26150 | 40.950 | 337 | 144 | 8 | 85 | 407 | 31 | 326 | 5.01e-76 | 240 |
| MS.gene57572.t1 | AT5G54070 | 52.713 | 258 | 108 | 7 | 66 | 320 | 46 | 292 | 8.55e-74 | 234 |
| MS.gene57572.t1 | AT3G22830 | 46.586 | 249 | 117 | 4 | 71 | 304 | 34 | 281 | 1.08e-73 | 236 |
| MS.gene57572.t1 | AT5G16820 | 47.391 | 230 | 94 | 3 | 68 | 280 | 2 | 221 | 6.22e-63 | 210 |
| MS.gene57572.t1 | AT5G16820 | 47.391 | 230 | 94 | 3 | 68 | 280 | 2 | 221 | 6.22e-63 | 210 |
| MS.gene57572.t1 | AT3G51910 | 48.792 | 207 | 96 | 3 | 88 | 293 | 19 | 216 | 8.44e-63 | 204 |
| MS.gene57572.t1 | AT4G17750 | 50.000 | 216 | 82 | 3 | 96 | 290 | 50 | 260 | 3.65e-62 | 209 |
| MS.gene57572.t1 | AT1G32330 | 47.186 | 231 | 106 | 3 | 85 | 299 | 24 | 254 | 7.42e-62 | 207 |
| MS.gene57572.t1 | AT1G32330 | 47.186 | 231 | 106 | 3 | 85 | 299 | 24 | 254 | 8.37e-62 | 207 |
| MS.gene57572.t1 | AT3G63350 | 39.850 | 266 | 150 | 3 | 71 | 327 | 1 | 265 | 8.35e-60 | 196 |
| MS.gene57572.t1 | AT3G02990 | 51.579 | 190 | 81 | 2 | 97 | 275 | 22 | 211 | 1.60e-57 | 196 |
| MS.gene57572.t1 | AT5G43840 | 45.503 | 189 | 96 | 1 | 84 | 272 | 5 | 186 | 1.33e-54 | 182 |
| MS.gene57572.t1 | AT5G03720 | 47.291 | 203 | 101 | 3 | 84 | 280 | 41 | 243 | 1.69e-54 | 186 |
| MS.gene57572.t1 | AT5G03720 | 47.291 | 203 | 101 | 3 | 84 | 280 | 41 | 243 | 1.69e-54 | 186 |
| MS.gene57572.t1 | AT1G67970 | 48.108 | 185 | 88 | 2 | 98 | 274 | 19 | 203 | 1.32e-49 | 172 |
| MS.gene57572.t1 | AT4G13980 | 43.716 | 183 | 100 | 1 | 99 | 278 | 24 | 206 | 2.08e-46 | 166 |
| MS.gene57572.t1 | AT3G24520 | 41.000 | 200 | 102 | 2 | 98 | 297 | 17 | 200 | 5.23e-43 | 154 |
| MS.gene57572.t1 | AT4G18880 | 44.865 | 185 | 94 | 2 | 90 | 266 | 7 | 191 | 7.48e-38 | 142 |
| MS.gene57572.t1 | AT5G45710 | 41.808 | 177 | 96 | 1 | 97 | 266 | 12 | 188 | 2.05e-37 | 139 |
| MS.gene57572.t1 | AT5G45710 | 41.808 | 177 | 96 | 1 | 97 | 266 | 12 | 188 | 2.05e-37 | 139 |
| MS.gene57572.t1 | AT1G46264 | 61.386 | 101 | 39 | 0 | 88 | 188 | 24 | 124 | 2.05e-37 | 139 |
| MS.gene57572.t1 | AT2G41690 | 61.290 | 93 | 36 | 0 | 96 | 188 | 38 | 130 | 6.96e-36 | 132 |
| MS.gene57572.t1 | AT4G11660 | 61.290 | 93 | 36 | 0 | 96 | 188 | 57 | 149 | 2.28e-35 | 134 |
| MS.gene57572.t1 | AT4G36990 | 62.366 | 93 | 35 | 0 | 96 | 188 | 12 | 104 | 3.46e-35 | 132 |
| MS.gene57572.t1 | AT5G62020 | 58.416 | 101 | 41 | 1 | 96 | 196 | 21 | 120 | 4.41e-35 | 132 |
| MS.gene57572.t1 | AT2G26150 | 29.195 | 298 | 136 | 9 | 124 | 407 | 35 | 271 | 4.02e-27 | 110 |
| MS.gene57572.t1 | AT2G26150 | 29.195 | 298 | 136 | 9 | 124 | 407 | 35 | 271 | 4.02e-27 | 110 |
| MS.gene57572.t1 | AT4G18870 | 42.045 | 88 | 51 | 0 | 98 | 185 | 146 | 233 | 1.92e-18 | 85.5 |
| MS.gene57572.t1 | AT4G18870 | 43.678 | 87 | 48 | 1 | 99 | 185 | 14 | 99 | 1.88e-17 | 82.8 |
| MS.gene57572.t1 | AT1G77570 | 37.778 | 90 | 53 | 2 | 99 | 185 | 27 | 116 | 2.07e-12 | 65.1 |
| MS.gene57572.t1 | AT4G19630 | 38.542 | 96 | 52 | 3 | 95 | 185 | 8 | 101 | 1.52e-11 | 62.0 |
Find 87 sgRNAs with CRISPR-Local
Find 120 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCCAAAAGGCCATTGTTTA+TGG | 0.168710 | 8.4:+9016998 | MS.gene57572:CDS |
| GTATTTAGGAAGAAGAATTT+TGG | 0.245282 | 8.4:-9015815 | None:intergenic |
| GAAACAATCGGGTCAGTTTC+CGG | 0.255207 | 8.4:-9015742 | None:intergenic |
| AGATCAGAACATGTTGAAAT+TGG | 0.272987 | 8.4:+9016857 | MS.gene57572:CDS |
| CTCCATAAACAATGGCCTTT+TGG | 0.280439 | 8.4:-9017000 | None:intergenic |
| TGTAACCCTTCCATTGGTTT+AGG | 0.283169 | 8.4:-9015667 | None:intergenic |
| TTATTATGCTTAAAGTATTT+AGG | 0.297550 | 8.4:-9015829 | None:intergenic |
| TAATATCTATCTTGAATTAG+AGG | 0.304710 | 8.4:+9017385 | MS.gene57572:CDS |
| TGTTATGTCAGAGAAGCTTT+TGG | 0.350236 | 8.4:+9017319 | MS.gene57572:CDS |
| TCTGTCACATGCAGGGGTTT+AGG | 0.362833 | 8.4:+9016670 | MS.gene57572:intron |
| GATAAGTAAGCCAACAGATT+GGG | 0.381656 | 8.4:+9017412 | MS.gene57572:CDS |
| CCTTGTGTTCCAAGCAATCT+AGG | 0.385680 | 8.4:-9017086 | None:intergenic |
| TTCTGTTGTTGATGAAGAAA+TGG | 0.389880 | 8.4:+9017349 | MS.gene57572:CDS |
| CGTTGGGAGTTTGCAAATGA+AGG | 0.392464 | 8.4:+9016708 | MS.gene57572:CDS |
| TGTAGCACCTTCTTCTTCTT+CGG | 0.398248 | 8.4:-9015569 | None:intergenic |
| TATTCAACTCACAAATGTTC+AGG | 0.406470 | 8.4:+9016917 | MS.gene57572:CDS |
| TAATGTTGATTATAGGAGTC+AGG | 0.409936 | 8.4:+9017112 | MS.gene57572:CDS |
| AAACTGACCCGATTGTTTCA+TGG | 0.421449 | 8.4:+9015746 | MS.gene57572:CDS |
| TGGATCTGCTAGTGGATTGG+TGG | 0.433958 | 8.4:+9017436 | MS.gene57572:CDS |
| GGTGGTTACGACGGTGAAAT+CGG | 0.435742 | 8.4:+9015430 | MS.gene57572:CDS |
| TAGCTCTTTACATGACTTGA+GGG | 0.437021 | 8.4:+9017247 | MS.gene57572:CDS |
| GTTTGAAATGGTGGAGGATC+CGG | 0.440036 | 8.4:+9015723 | MS.gene57572:CDS |
| GTTAAAAGACCTAGATTGCT+TGG | 0.444562 | 8.4:+9017077 | MS.gene57572:CDS |
| AAGGTACTAATGTTGATTAT+AGG | 0.444857 | 8.4:+9017105 | MS.gene57572:CDS |
| AAATATGAACACTGGTAGAA+TGG | 0.455673 | 8.4:+9017187 | MS.gene57572:CDS |
| GAAGCACTCCATGAAACAAT+CGG | 0.466108 | 8.4:-9015754 | None:intergenic |
| TCTCAAGAAGACGTTTGAAA+TGG | 0.467110 | 8.4:+9015711 | MS.gene57572:CDS |
| TGATAAGTAAGCCAACAGAT+TGG | 0.468059 | 8.4:+9017411 | MS.gene57572:CDS |
| TCCTCACCAAAATGGCCAAA+AGG | 0.470362 | 8.4:+9016985 | MS.gene57572:CDS |
| TCTTCCATGGGAGTAGAAAC+AGG | 0.475802 | 8.4:-9017215 | None:intergenic |
| GAAGGTTGATTCAGACCGTT+GGG | 0.482934 | 8.4:+9016692 | MS.gene57572:CDS |
| CAAATGAAGGATTTCAAGCA+GGG | 0.484602 | 8.4:+9016721 | MS.gene57572:CDS |
| GACTTACATAGGTGTTGAGC+TGG | 0.485810 | 8.4:-9015870 | None:intergenic |
| ATTTGTTGAAGAACATAAGA+AGG | 0.488953 | 8.4:+9016751 | MS.gene57572:CDS |
| ACCTCATGTAACCCTTCCAT+TGG | 0.493568 | 8.4:-9015673 | None:intergenic |
| GCACTGTCGTTGAATAACTC+TGG | 0.499469 | 8.4:-9015487 | None:intergenic |
| GGGTGGATCTGCTAGTGGAT+TGG | 0.502731 | 8.4:+9017433 | MS.gene57572:CDS |
| GCAAATGAAGGATTTCAAGC+AGG | 0.502811 | 8.4:+9016720 | MS.gene57572:CDS |
| ATTCAAAAGATTAAGCGAAA+AGG | 0.508398 | 8.4:+9017029 | MS.gene57572:CDS |
| TTACATGACTTGAGGGCTTG+TGG | 0.510182 | 8.4:+9017254 | MS.gene57572:CDS |
| AACAAAATGGCGGTGGCACA+CGG | 0.510707 | 8.4:+9015409 | None:intergenic |
| ACTTTGCAATCTGAATTGAA+TGG | 0.510922 | 8.4:+9017152 | MS.gene57572:CDS |
| CAAGAAGACGTTTGAAATGG+TGG | 0.512314 | 8.4:+9015714 | MS.gene57572:CDS |
| GTTTCTACTCCCATGGAAGA+TGG | 0.512936 | 8.4:+9017218 | MS.gene57572:CDS |
| CGCAATTCATATCAAAGAAG+AGG | 0.515252 | 8.4:+9015600 | MS.gene57572:CDS |
| GGAAGGTTGATTCAGACCGT+TGG | 0.515312 | 8.4:+9016691 | MS.gene57572:CDS |
| GTAGCTCTTTACATGACTTG+AGG | 0.518635 | 8.4:+9017246 | MS.gene57572:CDS |
| TCAAACGTCTTCTTGAGAAA+CGG | 0.525722 | 8.4:-9015706 | None:intergenic |
| AATTGGAAATTCTGAAGCTA+AGG | 0.526195 | 8.4:+9016874 | MS.gene57572:CDS |
| TTCGGTTGAAACGTCGTCGT+AGG | 0.530109 | 8.4:-9015527 | None:intergenic |
| GCTACACAGTCCATCTTCCA+TGG | 0.535967 | 8.4:-9017228 | None:intergenic |
| GGATCTGCTAGTGGATTGGT+GGG | 0.540365 | 8.4:+9017437 | MS.gene57572:CDS |
| ACTCAGGTTTCCTAAACCAA+TGG | 0.540511 | 8.4:+9015657 | MS.gene57572:CDS |
| ACCACCTGTTTCTACTCCCA+TGG | 0.552610 | 8.4:+9017211 | MS.gene57572:CDS |
| AGGTTTCCTAAACCAATGGA+AGG | 0.554746 | 8.4:+9015661 | MS.gene57572:CDS |
| TCCACTTCAGATTCAACGCA+AGG | 0.559576 | 8.4:-9016822 | None:intergenic |
| CTTCATTTGCAAACTCCCAA+CGG | 0.561522 | 8.4:-9016707 | None:intergenic |
| CCTAGATTGCTTGGAACACA+AGG | 0.567220 | 8.4:+9017086 | MS.gene57572:CDS |
| ACAAATAAACAAAATGGCGG+TGG | 0.567551 | 8.4:+9015402 | None:intergenic |
| TCACATGCAGGGGTTTAGGA+AGG | 0.572288 | 8.4:+9016674 | MS.gene57572:intron |
| ATAAGTAAGCCAACAGATTG+GGG | 0.574399 | 8.4:+9017413 | MS.gene57572:CDS |
| AAGCACTCCATGAAACAATC+GGG | 0.577813 | 8.4:-9015753 | None:intergenic |
| AAGTATAACAAACTGCATCA+AGG | 0.578236 | 8.4:+9016780 | MS.gene57572:CDS |
| AATGTTGATTATAGGAGTCA+GGG | 0.582952 | 8.4:+9017113 | MS.gene57572:CDS |
| TTTCCTGAAAATATGAACAC+TGG | 0.585214 | 8.4:+9017179 | MS.gene57572:CDS |
| CTAACGCTTCATCTTCACTC+AGG | 0.586633 | 8.4:+9015641 | MS.gene57572:CDS |
| GCAGATCCACCCCAATCTGT+TGG | 0.588399 | 8.4:-9017422 | None:intergenic |
| GTCTTCTTGAGAAACGGTGG+TGG | 0.589533 | 8.4:-9015700 | None:intergenic |
| ATGGAAGGGTTACATGAGGT+TGG | 0.601818 | 8.4:+9015676 | MS.gene57572:CDS |
| GTACTAACCGAAGAAGAAGA+AGG | 0.604108 | 8.4:+9015562 | MS.gene57572:CDS |
| TCCTTGCGTTGAATCTGAAG+TGG | 0.604555 | 8.4:+9016821 | MS.gene57572:CDS |
| GAAGAGGTTCTTGAAGAAGA+CGG | 0.609483 | 8.4:+9015616 | MS.gene57572:CDS |
| CTACACAGTCCATCTTCCAT+GGG | 0.610941 | 8.4:-9017227 | None:intergenic |
| ATTATCTGCTCCATAAACAA+TGG | 0.622243 | 8.4:-9017008 | None:intergenic |
| GATTGGGGTGGATCTGCTAG+TGG | 0.632406 | 8.4:+9017428 | MS.gene57572:CDS |
| GGTTTCCTAAACCAATGGAA+GGG | 0.633308 | 8.4:+9015662 | MS.gene57572:CDS |
| GAAGACGTTTGAAATGGTGG+AGG | 0.633963 | 8.4:+9015717 | MS.gene57572:CDS |
| CATGACTTGAGGGCTTGTGG+TGG | 0.643012 | 8.4:+9017257 | MS.gene57572:CDS |
| GTGGCACACGGTGGTTACGA+CGG | 0.645161 | 8.4:+9015421 | MS.gene57572:CDS |
| AACGTCTTCTTGAGAAACGG+TGG | 0.656391 | 8.4:-9015703 | None:intergenic |
| AGTAAGCCAACAGATTGGGG+TGG | 0.660771 | 8.4:+9017416 | MS.gene57572:CDS |
| ACCAATGGAAGGGTTACATG+AGG | 0.667282 | 8.4:+9015672 | MS.gene57572:CDS |
| GGTTACGACGGTGAAATCGG+TGG | 0.688203 | 8.4:+9015433 | MS.gene57572:CDS |
| AGTTGATGCTAATATAGACA+TGG | 0.689403 | 8.4:+9017055 | MS.gene57572:CDS |
| TATAACAAACTGCATCAAGG+AGG | 0.715319 | 8.4:+9016783 | MS.gene57572:CDS |
| AAAATGGCGGTGGCACACGG+TGG | 0.747694 | 8.4:+9015412 | None:intergenic |
| TCCATGGGAGTAGAAACAGG+TGG | 0.762840 | 8.4:-9017212 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAAATGATTCATATTTAT+AGG | + | chr8.4:9016059-9016078 | MS.gene57572:intron | 10.0% |
| !! | TAAAATGATTCATATTTATA+GGG | + | chr8.4:9016060-9016079 | MS.gene57572:intron | 10.0% |
| !!! | TTATTATGCTTAAAGTATTT+AGG | - | chr8.4:9015832-9015851 | None:intergenic | 15.0% |
| !!! | TTTTTTCATGTTTTTTTACA+TGG | - | chr8.4:9016361-9016380 | None:intergenic | 15.0% |
| !! | AAATCCAGAATAAATTCAAA+TGG | - | chr8.4:9016414-9016433 | None:intergenic | 20.0% |
| !! | TAATATCTATCTTGAATTAG+AGG | + | chr8.4:9017385-9017404 | MS.gene57572:CDS | 20.0% |
| !!! | AGAATTTTTTTGACTTACAT+AGG | - | chr8.4:9015884-9015903 | None:intergenic | 20.0% |
| !!! | GCAAAAAACAAAATAGATTT+TGG | - | chr8.4:9016493-9016512 | None:intergenic | 20.0% |
| !!! | TGTACAATTTAAATCAGTTT+TGG | + | chr8.4:9015949-9015968 | MS.gene57572:intron | 20.0% |
| ! | ACATTAAATTCGAACTTCAT+TGG | + | chr8.4:9016306-9016325 | MS.gene57572:intron | 25.0% |
| ! | ATTCAAAAGATTAAGCGAAA+AGG | + | chr8.4:9017029-9017048 | MS.gene57572:CDS | 25.0% |
| !! | AAGGTACTAATGTTGATTAT+AGG | + | chr8.4:9017105-9017124 | MS.gene57572:CDS | 25.0% |
| !! | ATTTGTTGAAGAACATAAGA+AGG | + | chr8.4:9016751-9016770 | MS.gene57572:CDS | 25.0% |
| !! | GTATTTAGGAAGAAGAATTT+TGG | - | chr8.4:9015818-9015837 | None:intergenic | 25.0% |
| !!! | AATAACTCTGGTTTTATTGA+TGG | - | chr8.4:9015478-9015497 | None:intergenic | 25.0% |
| !!! | ATAACTCTGGTTTTATTGAT+GGG | - | chr8.4:9015477-9015496 | None:intergenic | 25.0% |
| !!! | TAGTACTGATTCTTTTCTTT+CGG | - | chr8.4:9015548-9015567 | None:intergenic | 25.0% |
| !!! | TTACATGAACTCACTTTTAT+AGG | + | chr8.4:9016265-9016284 | MS.gene57572:intron | 25.0% |
| !!! | TTTTTTGCAACCACAAAAAT+AGG | + | chr8.4:9016505-9016524 | MS.gene57572:intron | 25.0% |
| AAATATGAACACTGGTAGAA+TGG | + | chr8.4:9017187-9017206 | MS.gene57572:CDS | 30.0% | |
| AAATCAACTCAATGCATGTT+TGG | - | chr8.4:9016004-9016023 | None:intergenic | 30.0% | |
| AAGTATAACAAACTGCATCA+AGG | + | chr8.4:9016780-9016799 | MS.gene57572:CDS | 30.0% | |
| AATGCATGTTTGGTATAATG+TGG | - | chr8.4:9015994-9016013 | None:intergenic | 30.0% | |
| ACTTTGCAATCTGAATTGAA+TGG | + | chr8.4:9017152-9017171 | MS.gene57572:CDS | 30.0% | |
| AGATCAGAACATGTTGAAAT+TGG | + | chr8.4:9016857-9016876 | MS.gene57572:CDS | 30.0% | |
| ATTATCTGCTCCATAAACAA+TGG | - | chr8.4:9017011-9017030 | None:intergenic | 30.0% | |
| TATTCAACTCACAAATGTTC+AGG | + | chr8.4:9016917-9016936 | MS.gene57572:CDS | 30.0% | |
| TTCGCCATTTGAATTTATTC+TGG | + | chr8.4:9016407-9016426 | MS.gene57572:intron | 30.0% | |
| TTTCCTGAAAATATGAACAC+TGG | + | chr8.4:9017179-9017198 | MS.gene57572:CDS | 30.0% | |
| ! | AATGTTGATTATAGGAGTCA+GGG | + | chr8.4:9017113-9017132 | MS.gene57572:CDS | 30.0% |
| ! | AGTTGATGCTAATATAGACA+TGG | + | chr8.4:9017055-9017074 | MS.gene57572:CDS | 30.0% |
| ! | TAATGTTGATTATAGGAGTC+AGG | + | chr8.4:9017112-9017131 | MS.gene57572:CDS | 30.0% |
| ! | TTCTGTTGTTGATGAAGAAA+TGG | + | chr8.4:9017349-9017368 | MS.gene57572:CDS | 30.0% |
| !! | AATTGGAAATTCTGAAGCTA+AGG | + | chr8.4:9016874-9016893 | MS.gene57572:CDS | 30.0% |
| !! | TTAAATCAGTTTTGGCATCA+CGG | + | chr8.4:9015957-9015976 | MS.gene57572:intron | 30.0% |
| !!! | TCGTGATAGTTTTGTTGTTT+GGG | + | chr8.4:9015777-9015796 | MS.gene57572:CDS | 30.0% |
| ATAAGTAAGCCAACAGATTG+GGG | + | chr8.4:9017413-9017432 | MS.gene57572:CDS | 35.0% | |
| CAAATGAAGGATTTCAAGCA+GGG | + | chr8.4:9016721-9016740 | MS.gene57572:CDS | 35.0% | |
| CGCAATTCATATCAAAGAAG+AGG | + | chr8.4:9015600-9015619 | MS.gene57572:CDS | 35.0% | |
| GATAAGTAAGCCAACAGATT+GGG | + | chr8.4:9017412-9017431 | MS.gene57572:CDS | 35.0% | |
| GTTAAAAGACCTAGATTGCT+TGG | + | chr8.4:9017077-9017096 | MS.gene57572:CDS | 35.0% | |
| TAGCTCTTTACATGACTTGA+GGG | + | chr8.4:9017247-9017266 | MS.gene57572:CDS | 35.0% | |
| TATAACAAACTGCATCAAGG+AGG | + | chr8.4:9016783-9016802 | MS.gene57572:CDS | 35.0% | |
| TATAGGGAGCTATGAATGTT+TGG | + | chr8.4:9016076-9016095 | MS.gene57572:intron | 35.0% | |
| TCAAACGTCTTCTTGAGAAA+CGG | - | chr8.4:9015709-9015728 | None:intergenic | 35.0% | |
| TCTCAAGAAGACGTTTGAAA+TGG | + | chr8.4:9015711-9015730 | MS.gene57572:CDS | 35.0% | |
| TGATAAGTAAGCCAACAGAT+TGG | + | chr8.4:9017411-9017430 | MS.gene57572:CDS | 35.0% | |
| TTATGAATCTGTCACATGCA+GGG | + | chr8.4:9016663-9016682 | MS.gene57572:intron | 35.0% | |
| TTTATGAATCTGTCACATGC+AGG | + | chr8.4:9016662-9016681 | MS.gene57572:intron | 35.0% | |
| TTTGCAACCACAAAAATAGG+TGG | + | chr8.4:9016508-9016527 | MS.gene57572:intron | 35.0% | |
| ! | CTACCAGTGTTCATATTTTC+AGG | - | chr8.4:9017185-9017204 | None:intergenic | 35.0% |
| ! | GTTATGTCAGAGAAGCTTTT+GGG | + | chr8.4:9017320-9017339 | MS.gene57572:CDS | 35.0% |
| ! | TGTTATGTCAGAGAAGCTTT+TGG | + | chr8.4:9017319-9017338 | MS.gene57572:CDS | 35.0% |
| ! | TTATGTCAGAGAAGCTTTTG+GGG | + | chr8.4:9017321-9017340 | MS.gene57572:CDS | 35.0% |
| ! | TTCAACATCTCCTCTAAAAG+AGG | - | chr8.4:9016104-9016123 | None:intergenic | 35.0% |
| !! | ATGTTTGGTTCCTCTTTTAG+AGG | + | chr8.4:9016091-9016110 | MS.gene57572:intron | 35.0% |
| !!! | CTCGTGATAGTTTTGTTGTT+TGG | + | chr8.4:9015776-9015795 | MS.gene57572:CDS | 35.0% |
| !!! | CTGGTTTTATTGATGGGATT+AGG | - | chr8.4:9015471-9015490 | None:intergenic | 35.0% |
| !!! | GCTATTTTTCCTCACCAAAA+TGG | + | chr8.4:9016977-9016996 | MS.gene57572:CDS | 35.0% |
| !!! | TTAGTCTCCACCTATTTTTG+TGG | - | chr8.4:9016518-9016537 | None:intergenic | 35.0% |
| AAACTGACCCGATTGTTTCA+TGG | + | chr8.4:9015746-9015765 | MS.gene57572:CDS | 40.0% | |
| AAGCACTCCATGAAACAATC+GGG | - | chr8.4:9015756-9015775 | None:intergenic | 40.0% | |
| ACTCAGGTTTCCTAAACCAA+TGG | + | chr8.4:9015657-9015676 | MS.gene57572:CDS | 40.0% | |
| AGGTTTCCTAAACCAATGGA+AGG | + | chr8.4:9015661-9015680 | MS.gene57572:CDS | 40.0% | |
| CTTCATTTGCAAACTCCCAA+CGG | - | chr8.4:9016710-9016729 | None:intergenic | 40.0% | |
| GAAGAGGTTCTTGAAGAAGA+CGG | + | chr8.4:9015616-9015635 | MS.gene57572:CDS | 40.0% | |
| GCAAATGAAGGATTTCAAGC+AGG | + | chr8.4:9016720-9016739 | MS.gene57572:CDS | 40.0% | |
| GGTGGAGACTAAAAATCAAG+AGG | + | chr8.4:9016526-9016545 | MS.gene57572:intron | 40.0% | |
| GGTTTCCTAAACCAATGGAA+GGG | + | chr8.4:9015662-9015681 | MS.gene57572:CDS | 40.0% | |
| GTACTAACCGAAGAAGAAGA+AGG | + | chr8.4:9015562-9015581 | MS.gene57572:CDS | 40.0% | |
| GTAGCTCTTTACATGACTTG+AGG | + | chr8.4:9017246-9017265 | MS.gene57572:CDS | 40.0% | |
| TATGAATCTGTCACATGCAG+GGG | + | chr8.4:9016664-9016683 | MS.gene57572:intron | 40.0% | |
| TGTAGCACCTTCTTCTTCTT+CGG | - | chr8.4:9015572-9015591 | None:intergenic | 40.0% | |
| ! | CAAGAAGACGTTTGAAATGG+TGG | + | chr8.4:9015714-9015733 | MS.gene57572:CDS | 40.0% |
| ! | CTCCATAAACAATGGCCTTT+TGG | - | chr8.4:9017003-9017022 | None:intergenic | 40.0% |
| ! | GAAGCACTCCATGAAACAAT+CGG | - | chr8.4:9015757-9015776 | None:intergenic | 40.0% |
| ! | TATGTCAGAGAAGCTTTTGG+GGG | + | chr8.4:9017322-9017341 | MS.gene57572:CDS | 40.0% |
| !! | TGTAACCCTTCCATTGGTTT+AGG | - | chr8.4:9015670-9015689 | None:intergenic | 40.0% |
| AACGTCTTCTTGAGAAACGG+TGG | - | chr8.4:9015706-9015725 | None:intergenic | 45.0% | |
| ACCAATGGAAGGGTTACATG+AGG | + | chr8.4:9015672-9015691 | MS.gene57572:CDS | 45.0% | |
| ACCTCATGTAACCCTTCCAT+TGG | - | chr8.4:9015676-9015695 | None:intergenic | 45.0% | |
| ATGGAAGGGTTACATGAGGT+TGG | + | chr8.4:9015676-9015695 | MS.gene57572:CDS | 45.0% | |
| CCTAGATTGCTTGGAACACA+AGG | + | chr8.4:9017086-9017105 | MS.gene57572:CDS | 45.0% | |
| CCTTGTGTTCCAAGCAATCT+AGG | - | chr8.4:9017089-9017108 | None:intergenic | 45.0% | |
| CGTTGGGAGTTTGCAAATGA+AGG | + | chr8.4:9016708-9016727 | MS.gene57572:CDS | 45.0% | |
| CTAACGCTTCATCTTCACTC+AGG | + | chr8.4:9015641-9015660 | MS.gene57572:CDS | 45.0% | |
| CTACACAGTCCATCTTCCAT+GGG | - | chr8.4:9017230-9017249 | None:intergenic | 45.0% | |
| GAAACAATCGGGTCAGTTTC+CGG | - | chr8.4:9015745-9015764 | None:intergenic | 45.0% | |
| GCACTGTCGTTGAATAACTC+TGG | - | chr8.4:9015490-9015509 | None:intergenic | 45.0% | |
| GGCCAAAAGGCCATTGTTTA+TGG | + | chr8.4:9016998-9017017 | MS.gene57572:CDS | 45.0% | |
| GTTTCTACTCCCATGGAAGA+TGG | + | chr8.4:9017218-9017237 | MS.gene57572:CDS | 45.0% | |
| TCCACTTCAGATTCAACGCA+AGG | - | chr8.4:9016825-9016844 | None:intergenic | 45.0% | |
| TCCTCACCAAAATGGCCAAA+AGG | + | chr8.4:9016985-9017004 | MS.gene57572:CDS | 45.0% | |
| TCCTTGCGTTGAATCTGAAG+TGG | + | chr8.4:9016821-9016840 | MS.gene57572:CDS | 45.0% | |
| TCTTCCATGGGAGTAGAAAC+AGG | - | chr8.4:9017218-9017237 | None:intergenic | 45.0% | |
| TTACATGACTTGAGGGCTTG+TGG | + | chr8.4:9017254-9017273 | MS.gene57572:CDS | 45.0% | |
| ! | CAATGGCCTTTTGGCCATTT+TGG | - | chr8.4:9016994-9017013 | None:intergenic | 45.0% |
| ! | GAAGACGTTTGAAATGGTGG+AGG | + | chr8.4:9015717-9015736 | MS.gene57572:CDS | 45.0% |
| ! | GAAGGTTGATTCAGACCGTT+GGG | + | chr8.4:9016692-9016711 | MS.gene57572:CDS | 45.0% |
| ! | GTTTGAAATGGTGGAGGATC+CGG | + | chr8.4:9015723-9015742 | MS.gene57572:CDS | 45.0% |
| !! | GACTTACATAGGTGTTGAGC+TGG | - | chr8.4:9015873-9015892 | None:intergenic | 45.0% |
| AGTAAGCCAACAGATTGGGG+TGG | + | chr8.4:9017416-9017435 | MS.gene57572:CDS | 50.0% | |
| GCTACACAGTCCATCTTCCA+TGG | - | chr8.4:9017231-9017250 | None:intergenic | 50.0% | |
| GGTGGTTACGACGGTGAAAT+CGG | + | chr8.4:9015430-9015449 | MS.gene57572:CDS | 50.0% | |
| GTCTTCTTGAGAAACGGTGG+TGG | - | chr8.4:9015703-9015722 | None:intergenic | 50.0% | |
| TCACATGCAGGGGTTTAGGA+AGG | + | chr8.4:9016674-9016693 | MS.gene57572:intron | 50.0% | |
| TCCATGGGAGTAGAAACAGG+TGG | - | chr8.4:9017215-9017234 | None:intergenic | 50.0% | |
| TCTGTCACATGCAGGGGTTT+AGG | + | chr8.4:9016670-9016689 | MS.gene57572:intron | 50.0% | |
| TTCGGTTGAAACGTCGTCGT+AGG | - | chr8.4:9015530-9015549 | None:intergenic | 50.0% | |
| ! | GGAAGGTTGATTCAGACCGT+TGG | + | chr8.4:9016691-9016710 | MS.gene57572:CDS | 50.0% |
| !! | ACCACCTGTTTCTACTCCCA+TGG | + | chr8.4:9017211-9017230 | MS.gene57572:CDS | 50.0% |
| !! | GCCTTTTGGCCATTTTGGTG+AGG | - | chr8.4:9016989-9017008 | None:intergenic | 50.0% |
| !! | GGATCTGCTAGTGGATTGGT+GGG | + | chr8.4:9017437-9017456 | MS.gene57572:CDS | 50.0% |
| !! | TGGATCTGCTAGTGGATTGG+TGG | + | chr8.4:9017436-9017455 | MS.gene57572:CDS | 50.0% |
| CATGACTTGAGGGCTTGTGG+TGG | + | chr8.4:9017257-9017276 | MS.gene57572:CDS | 55.0% | |
| GATTGGGGTGGATCTGCTAG+TGG | + | chr8.4:9017428-9017447 | MS.gene57572:CDS | 55.0% | |
| GCAGATCCACCCCAATCTGT+TGG | - | chr8.4:9017425-9017444 | None:intergenic | 55.0% | |
| GGTTACGACGGTGAAATCGG+TGG | + | chr8.4:9015433-9015452 | MS.gene57572:CDS | 55.0% | |
| ! | GGGTGGATCTGCTAGTGGAT+TGG | + | chr8.4:9017433-9017452 | MS.gene57572:CDS | 55.0% |
| GTGGCACACGGTGGTTACGA+CGG | + | chr8.4:9015421-9015440 | MS.gene57572:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 9015415 | 9017472 | 9015415 | ID=MS.gene57572 |
| chr8.4 | mRNA | 9015415 | 9017472 | 9015415 | ID=MS.gene57572.t1;Parent=MS.gene57572 |
| chr8.4 | exon | 9015415 | 9015885 | 9015415 | ID=MS.gene57572.t1.exon1;Parent=MS.gene57572.t1 |
| chr8.4 | CDS | 9015415 | 9015885 | 9015415 | ID=cds.MS.gene57572.t1;Parent=MS.gene57572.t1 |
| chr8.4 | exon | 9016684 | 9017472 | 9016684 | ID=MS.gene57572.t1.exon2;Parent=MS.gene57572.t1 |
| chr8.4 | CDS | 9016684 | 9017472 | 9016684 | ID=cds.MS.gene57572.t1;Parent=MS.gene57572.t1 |
| Gene Sequence |
| Protein sequence |
