Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59742.t1 | XP_003628077.1 | 85 | 460 | 63 | 1 | 1 | 454 | 1 | 460 | 3.50E-230 | 807.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59742.t1 | P0C897 | 23.0 | 496 | 257 | 15 | 44 | 451 | 61 | 519 | 8.4e-15 | 83.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59742.t1 | G7LJ67 | 85.0 | 460 | 63 | 1 | 1 | 454 | 1 | 460 | 2.5e-230 | 807.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049142 | MS.gene59742 | 0.801332 | 9.47E-49 | -1.69E-46 |
| MS.gene049159 | MS.gene59742 | 0.807748 | 4.34E-50 | -1.69E-46 |
| MS.gene049435 | MS.gene59742 | 0.802741 | 4.86E-49 | -1.69E-46 |
| MS.gene051719 | MS.gene59742 | 0.801229 | 9.93E-49 | -1.69E-46 |
| MS.gene052519 | MS.gene59742 | 0.801192 | 1.01E-48 | -1.69E-46 |
| MS.gene052863 | MS.gene59742 | 0.801541 | 8.58E-49 | -1.69E-46 |
| MS.gene053008 | MS.gene59742 | 0.80302 | 4.26E-49 | -1.69E-46 |
| MS.gene054775 | MS.gene59742 | 0.800798 | 1.22E-48 | -1.69E-46 |
| MS.gene055818 | MS.gene59742 | 0.82004 | 8.46E-53 | -1.69E-46 |
| MS.gene055926 | MS.gene59742 | 0.810402 | 1.17E-50 | -1.69E-46 |
| MS.gene057444 | MS.gene59742 | 0.810642 | 1.04E-50 | -1.69E-46 |
| MS.gene057693 | MS.gene59742 | 0.806347 | 8.60E-50 | -1.69E-46 |
| MS.gene060803 | MS.gene59742 | 0.803735 | 3.03E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59742.t1 | MTR_8g042170 | 86.304 | 460 | 57 | 1 | 1 | 454 | 1 | 460 | 0.0 | 825 |
| MS.gene59742.t1 | MTR_8g037923 | 74.786 | 468 | 104 | 3 | 1 | 454 | 6 | 473 | 0.0 | 731 |
| MS.gene59742.t1 | MTR_8g037920 | 72.209 | 421 | 89 | 3 | 47 | 454 | 7 | 412 | 0.0 | 622 |
| MS.gene59742.t1 | MTR_8g042400 | 81.948 | 349 | 60 | 2 | 107 | 454 | 1 | 347 | 0.0 | 585 |
| MS.gene59742.t1 | MTR_8g042070 | 83.895 | 267 | 30 | 3 | 202 | 455 | 1 | 267 | 3.04e-150 | 428 |
| MS.gene59742.t1 | MTR_8g042080 | 86.391 | 169 | 23 | 0 | 1 | 169 | 1 | 169 | 4.15e-105 | 310 |
| MS.gene59742.t1 | MTR_7g060320 | 38.446 | 489 | 236 | 13 | 1 | 453 | 7 | 466 | 3.61e-94 | 292 |
| MS.gene59742.t1 | MTR_7g059540 | 38.136 | 472 | 224 | 14 | 22 | 453 | 25 | 468 | 4.08e-94 | 292 |
| MS.gene59742.t1 | MTR_7g059540 | 37.295 | 488 | 216 | 15 | 22 | 453 | 24 | 477 | 1.13e-92 | 289 |
| MS.gene59742.t1 | MTR_7g060840 | 37.924 | 472 | 230 | 14 | 1 | 453 | 7 | 434 | 2.70e-92 | 286 |
| MS.gene59742.t1 | MTR_7g060115 | 37.660 | 470 | 222 | 13 | 22 | 457 | 23 | 455 | 2.72e-89 | 279 |
| MS.gene59742.t1 | MTR_0182s0060 | 35.795 | 528 | 241 | 13 | 8 | 453 | 7 | 518 | 4.98e-89 | 281 |
| MS.gene59742.t1 | MTR_7g446270 | 38.333 | 480 | 216 | 13 | 22 | 447 | 27 | 480 | 1.44e-88 | 278 |
| MS.gene59742.t1 | MTR_7g060020 | 38.950 | 457 | 210 | 15 | 22 | 457 | 27 | 435 | 2.18e-87 | 274 |
| MS.gene59742.t1 | MTR_7g060260 | 38.312 | 462 | 236 | 11 | 1 | 453 | 7 | 428 | 1.32e-86 | 271 |
| MS.gene59742.t1 | MTR_7g059810 | 38.528 | 462 | 211 | 14 | 24 | 453 | 26 | 446 | 5.37e-85 | 268 |
| MS.gene59742.t1 | MTR_7g060330 | 36.768 | 495 | 221 | 11 | 22 | 455 | 28 | 491 | 1.80e-83 | 265 |
| MS.gene59742.t1 | MTR_7g060370 | 37.022 | 497 | 238 | 14 | 1 | 453 | 7 | 472 | 8.68e-83 | 263 |
| MS.gene59742.t1 | MTR_7g060220 | 33.682 | 478 | 246 | 13 | 1 | 453 | 7 | 438 | 4.11e-73 | 237 |
| MS.gene59742.t1 | MTR_7g060160 | 33.547 | 468 | 234 | 13 | 1 | 457 | 6 | 407 | 6.28e-73 | 235 |
| MS.gene59742.t1 | MTR_7g059510 | 33.966 | 474 | 204 | 12 | 15 | 453 | 20 | 419 | 2.68e-72 | 234 |
| MS.gene59742.t1 | MTR_7g059525 | 51.282 | 195 | 80 | 2 | 259 | 453 | 106 | 285 | 2.57e-63 | 207 |
| MS.gene59742.t1 | MTR_8g042090 | 85.047 | 107 | 16 | 0 | 307 | 413 | 2 | 108 | 1.60e-61 | 196 |
| MS.gene59742.t1 | MTR_7g060310 | 45.041 | 242 | 108 | 4 | 212 | 453 | 273 | 489 | 1.08e-58 | 201 |
| MS.gene59742.t1 | MTR_7g060310 | 39.773 | 176 | 86 | 3 | 10 | 176 | 16 | 180 | 4.43e-34 | 134 |
| MS.gene59742.t1 | MTR_7g059480 | 51.795 | 195 | 79 | 3 | 259 | 453 | 596 | 775 | 1.49e-58 | 206 |
| MS.gene59742.t1 | MTR_7g059480 | 39.894 | 188 | 87 | 7 | 1 | 176 | 6 | 179 | 1.53e-30 | 125 |
| MS.gene59742.t1 | MTR_7g060080 | 46.667 | 195 | 89 | 2 | 259 | 453 | 14 | 193 | 1.51e-56 | 186 |
| MS.gene59742.t1 | MTR_7g059520 | 41.538 | 195 | 91 | 3 | 259 | 453 | 3 | 174 | 2.15e-43 | 151 |
| MS.gene59742.t1 | MTR_5g086340 | 28.941 | 425 | 241 | 13 | 45 | 451 | 51 | 432 | 4.65e-39 | 147 |
| MS.gene59742.t1 | MTR_7g059515 | 41.463 | 164 | 76 | 4 | 22 | 176 | 23 | 175 | 2.21e-34 | 128 |
| MS.gene59742.t1 | MTR_5g016570 | 27.273 | 462 | 262 | 15 | 26 | 451 | 103 | 526 | 3.83e-33 | 131 |
| MS.gene59742.t1 | MTR_8g042830 | 25.870 | 460 | 218 | 16 | 26 | 451 | 43 | 413 | 3.09e-28 | 116 |
| MS.gene59742.t1 | MTR_7g060060 | 42.029 | 138 | 63 | 4 | 48 | 176 | 1 | 130 | 1.09e-24 | 102 |
| MS.gene59742.t1 | MTR_5g086260 | 33.486 | 218 | 119 | 5 | 237 | 452 | 116 | 309 | 2.10e-24 | 103 |
| MS.gene59742.t1 | MTR_5g087580 | 30.672 | 238 | 140 | 6 | 214 | 451 | 2 | 214 | 1.21e-23 | 99.0 |
| MS.gene59742.t1 | MTR_2g036320 | 26.116 | 448 | 266 | 17 | 16 | 447 | 62 | 460 | 3.13e-22 | 99.8 |
| MS.gene59742.t1 | MTR_8g005325 | 25.359 | 418 | 265 | 13 | 44 | 443 | 74 | 462 | 4.00e-21 | 96.3 |
| MS.gene59742.t1 | MTR_8g005235 | 25.355 | 422 | 259 | 15 | 44 | 443 | 45 | 432 | 2.38e-20 | 93.6 |
| MS.gene59742.t1 | MTR_6g011050 | 24.122 | 427 | 271 | 14 | 26 | 447 | 26 | 404 | 5.09e-18 | 86.3 |
| MS.gene59742.t1 | MTR_7g059475 | 26.984 | 252 | 129 | 8 | 69 | 278 | 1 | 239 | 6.49e-18 | 83.6 |
| MS.gene59742.t1 | MTR_1g048070 | 32.716 | 162 | 73 | 5 | 21 | 175 | 25 | 157 | 1.41e-16 | 79.3 |
| MS.gene59742.t1 | MTR_2g036310 | 31.176 | 170 | 101 | 5 | 285 | 451 | 2 | 158 | 1.94e-16 | 77.0 |
| MS.gene59742.t1 | MTR_1g040690 | 24.473 | 474 | 280 | 18 | 26 | 451 | 29 | 472 | 2.15e-16 | 81.6 |
| MS.gene59742.t1 | MTR_6g010850 | 29.268 | 205 | 124 | 5 | 249 | 451 | 163 | 348 | 9.74e-16 | 79.0 |
| MS.gene59742.t1 | MTR_6g010810 | 25.113 | 442 | 257 | 22 | 26 | 451 | 24 | 407 | 1.93e-15 | 78.6 |
| MS.gene59742.t1 | MTR_0024s0080 | 25.517 | 435 | 259 | 16 | 26 | 451 | 27 | 405 | 1.65e-14 | 75.5 |
| MS.gene59742.t1 | MTR_6g010920 | 22.581 | 434 | 268 | 17 | 26 | 447 | 27 | 404 | 4.16e-14 | 74.3 |
| MS.gene59742.t1 | MTR_7g011980 | 28.125 | 192 | 117 | 5 | 262 | 451 | 169 | 341 | 2.10e-13 | 71.6 |
| MS.gene59742.t1 | MTR_7g060200 | 36.364 | 110 | 51 | 4 | 69 | 169 | 1 | 100 | 4.83e-13 | 68.2 |
| MS.gene59742.t1 | MTR_6g010890 | 22.637 | 455 | 259 | 20 | 26 | 447 | 27 | 421 | 7.02e-13 | 70.5 |
| MS.gene59742.t1 | MTR_3g079390 | 27.607 | 163 | 102 | 4 | 291 | 451 | 301 | 449 | 2.58e-12 | 68.9 |
| MS.gene59742.t1 | MTR_2g035640 | 24.662 | 296 | 177 | 7 | 157 | 447 | 90 | 344 | 1.00e-11 | 66.6 |
| MS.gene59742.t1 | MTR_8g005295 | 30.719 | 153 | 88 | 5 | 293 | 443 | 364 | 500 | 3.93e-11 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene59742.t1 | AT3G50120 | 27.352 | 457 | 263 | 17 | 26 | 451 | 105 | 523 | 5.61e-38 | 145 |
| MS.gene59742.t1 | AT3G50140 | 27.214 | 463 | 258 | 18 | 26 | 453 | 115 | 533 | 1.91e-35 | 138 |
| MS.gene59742.t1 | AT3G50150 | 26.490 | 453 | 263 | 14 | 26 | 451 | 92 | 501 | 4.62e-35 | 137 |
| MS.gene59742.t1 | AT3G50170 | 27.010 | 485 | 266 | 20 | 8 | 451 | 94 | 531 | 1.21e-33 | 133 |
| MS.gene59742.t1 | AT3G50160 | 27.945 | 433 | 260 | 13 | 26 | 451 | 106 | 493 | 2.04e-33 | 132 |
| MS.gene59742.t1 | AT3G50180 | 25.813 | 461 | 247 | 17 | 26 | 451 | 180 | 580 | 4.85e-31 | 126 |
| MS.gene59742.t1 | AT3G50130 | 28.289 | 456 | 261 | 16 | 26 | 453 | 141 | 558 | 8.82e-31 | 125 |
| MS.gene59742.t1 | AT3G50190 | 26.126 | 444 | 254 | 19 | 26 | 451 | 98 | 485 | 3.41e-28 | 117 |
| MS.gene59742.t1 | AT5G22550 | 25.586 | 469 | 274 | 19 | 26 | 451 | 46 | 482 | 2.69e-25 | 108 |
| MS.gene59742.t1 | AT3G47200 | 25.225 | 444 | 278 | 15 | 26 | 451 | 48 | 455 | 3.83e-24 | 105 |
| MS.gene59742.t1 | AT3G47200 | 25.225 | 444 | 278 | 15 | 26 | 451 | 48 | 455 | 3.83e-24 | 105 |
| MS.gene59742.t1 | AT2G36430 | 27.212 | 452 | 247 | 18 | 26 | 451 | 47 | 442 | 7.18e-24 | 103 |
| MS.gene59742.t1 | AT4G31980 | 23.423 | 444 | 261 | 15 | 26 | 451 | 295 | 677 | 1.80e-22 | 100 |
| MS.gene59742.t1 | AT3G44710 | 23.555 | 467 | 280 | 17 | 35 | 451 | 57 | 496 | 2.55e-22 | 100 |
| MS.gene59742.t1 | AT2G28580 | 26.545 | 437 | 261 | 15 | 26 | 451 | 111 | 498 | 1.96e-21 | 97.4 |
| MS.gene59742.t1 | AT2G28580 | 26.545 | 437 | 261 | 15 | 26 | 451 | 125 | 512 | 2.21e-21 | 97.4 |
| MS.gene59742.t1 | AT3G47250 | 23.502 | 434 | 264 | 18 | 45 | 451 | 80 | 472 | 2.28e-20 | 94.0 |
| MS.gene59742.t1 | AT3G47250 | 23.502 | 434 | 264 | 18 | 45 | 451 | 80 | 472 | 2.28e-20 | 94.0 |
| MS.gene59742.t1 | AT3G47250 | 23.502 | 434 | 264 | 18 | 45 | 451 | 80 | 472 | 2.28e-20 | 94.0 |
| MS.gene59742.t1 | AT5G22550 | 24.190 | 463 | 239 | 18 | 26 | 451 | 46 | 433 | 7.16e-20 | 92.0 |
| MS.gene59742.t1 | AT2G28580 | 25.828 | 453 | 261 | 16 | 26 | 451 | 43 | 447 | 9.73e-20 | 92.0 |
| MS.gene59742.t1 | AT2G28580 | 25.828 | 453 | 261 | 16 | 26 | 451 | 111 | 515 | 1.18e-19 | 92.0 |
| MS.gene59742.t1 | AT2G28580 | 25.828 | 453 | 261 | 16 | 26 | 451 | 125 | 529 | 1.46e-19 | 91.7 |
| MS.gene59742.t1 | AT2G44930 | 25.974 | 462 | 254 | 17 | 26 | 451 | 86 | 495 | 1.75e-17 | 85.1 |
| MS.gene59742.t1 | AT5G22540 | 24.122 | 427 | 250 | 18 | 26 | 429 | 33 | 408 | 1.63e-16 | 82.0 |
| MS.gene59742.t1 | AT5G22560 | 24.082 | 490 | 277 | 18 | 26 | 450 | 49 | 508 | 5.52e-16 | 80.5 |
| MS.gene59742.t1 | AT1G65985 | 24.295 | 461 | 284 | 19 | 15 | 439 | 5 | 436 | 1.66e-13 | 72.8 |
| MS.gene59742.t1 | AT1G65985 | 24.295 | 461 | 284 | 19 | 15 | 439 | 5 | 436 | 1.66e-13 | 72.8 |
Find 70 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACATACATATTTGTTTAAT+TGG | 0.057560 | 8.4:+57255701 | None:intergenic |
| TTGCTCGTTCTTTACCTTTA+TGG | 0.108954 | 8.4:-57256081 | MS.gene59742:CDS |
| ATGTTCTTTGCTGCACTTTA+TGG | 0.118793 | 8.4:-57256684 | MS.gene59742:CDS |
| TCAAAATTTGTTGCTGATTA+TGG | 0.126368 | 8.4:-57256857 | MS.gene59742:CDS |
| GGCAAAACTCTTCAATGATT+TGG | 0.156749 | 8.4:-57255973 | MS.gene59742:CDS |
| AGGAAGAAATTGATTCTAAT+TGG | 0.200457 | 8.4:-57256322 | MS.gene59742:CDS |
| CCCTTTCATTTACTTGATTT+TGG | 0.214130 | 8.4:-57256437 | MS.gene59742:CDS |
| CGAAGAAATGGGTATTGATT+TGG | 0.229534 | 8.4:-57256540 | MS.gene59742:CDS |
| GTTGGTCCAACTTAATGTTT+AGG | 0.243534 | 8.4:+57256633 | None:intergenic |
| GTTTAATTGGAATCTTTCTT+AGG | 0.249029 | 8.4:+57255714 | None:intergenic |
| GGAATCTTTCTTAGGATTTA+CGG | 0.262717 | 8.4:+57255722 | None:intergenic |
| AGCCAGTACTGCGGCTAAAA+AGG | 0.271485 | 8.4:+57255776 | None:intergenic |
| ACATTTCGTTCAAATCTAAA+TGG | 0.273730 | 8.4:-57256217 | MS.gene59742:CDS |
| TGGAATGGTAATAGTCAAAT+TGG | 0.282633 | 8.4:-57256471 | MS.gene59742:CDS |
| GAAGAAATGGGTATTGATTT+GGG | 0.286710 | 8.4:-57256539 | MS.gene59742:CDS |
| GCAAAACTCTTCAATGATTT+GGG | 0.287156 | 8.4:-57255972 | MS.gene59742:CDS |
| CTTTCATTCTTGTGATGAAT+AGG | 0.314368 | 8.4:+57256917 | None:intergenic |
| CTTTACCTTTATGGATTCAT+TGG | 0.318220 | 8.4:-57256072 | MS.gene59742:CDS |
| TTGGTCCAACTTAATGTTTA+GGG | 0.324477 | 8.4:+57256634 | None:intergenic |
| TTCTGATTGTTCTTCAAAGT+TGG | 0.333331 | 8.4:+57256397 | None:intergenic |
| GGACCAAATGATATGATCTT+TGG | 0.349653 | 8.4:+57256938 | None:intergenic |
| GATTTACGGTAAACCAGGTT+TGG | 0.362303 | 8.4:+57255736 | None:intergenic |
| TGAGATTCTATGCGAAGAAA+TGG | 0.363190 | 8.4:-57256552 | MS.gene59742:CDS |
| GAGAGCATTACAAGCTTCTA+TGG | 0.369567 | 8.4:-57256883 | MS.gene59742:CDS |
| TATTACCATTCCAAGTCGTT+TGG | 0.373545 | 8.4:+57256481 | None:intergenic |
| TTCAAATCTAAATGGTTTAG+TGG | 0.378791 | 8.4:-57256209 | MS.gene59742:CDS |
| TCTGATTGTTCTTCAAAGTT+GGG | 0.381344 | 8.4:+57256398 | None:intergenic |
| AATGGAACCCTTTGGATCCT+AGG | 0.389624 | 8.4:+57257001 | None:intergenic |
| GCTTGGAAGCGACGAAGAAT+TGG | 0.396278 | 8.4:-57255994 | MS.gene59742:CDS |
| ATAAGACAGATGTGTGGAAA+TGG | 0.406167 | 8.4:-57256244 | MS.gene59742:CDS |
| AACTTAATGTTTAGGGCATC+CGG | 0.415938 | 8.4:+57256641 | None:intergenic |
| GAGAATATATTTCTTGCTAT+AGG | 0.424105 | 8.4:+57255896 | None:intergenic |
| GTCAACCAATGAATCCATAA+AGG | 0.429644 | 8.4:+57256067 | None:intergenic |
| ATAATCGAATTTCGATTCCT+AGG | 0.434399 | 8.4:-57257018 | MS.gene59742:CDS |
| TCATAGTTGTAATGAACATC+TGG | 0.450347 | 8.4:+57256107 | None:intergenic |
| ACACCAAAGATCATATCATT+TGG | 0.453095 | 8.4:-57256941 | MS.gene59742:CDS |
| GTTTGCTTGGATATTGTTTG+TGG | 0.454526 | 8.4:-57256711 | MS.gene59742:CDS |
| GGTGTATTTCAAAACTTGCT+TGG | 0.456888 | 8.4:-57256011 | MS.gene59742:CDS |
| ACCAAAATCAAGTAAATGAA+AGG | 0.480260 | 8.4:+57256436 | None:intergenic |
| TTCACTGAGGATGCAATTGA+AGG | 0.480560 | 8.4:-57256749 | MS.gene59742:CDS |
| TCAAGTAAATGAAAGGGCTT+AGG | 0.481850 | 8.4:+57256443 | None:intergenic |
| GCTTGGATATTGTTTGTGGA+TGG | 0.481920 | 8.4:-57256707 | MS.gene59742:CDS |
| GTAAAAGAGCTTAGATCAGC+TGG | 0.484091 | 8.4:-57256032 | MS.gene59742:CDS |
| TGGACCAACTGATGCACATA+TGG | 0.485178 | 8.4:-57256619 | MS.gene59742:CDS |
| ACAATGATAACGAGTTTGCT+TGG | 0.498692 | 8.4:-57256724 | MS.gene59742:CDS |
| CTTAGGATTTACGGTAAACC+AGG | 0.504097 | 8.4:+57255731 | None:intergenic |
| AAGAATGAAAGTCTGAAAGA+AGG | 0.520480 | 8.4:-57256905 | MS.gene59742:CDS |
| GAGATTCTATGCGAAGAAAT+GGG | 0.527513 | 8.4:-57256551 | MS.gene59742:CDS |
| TTCTTACTTTCATCCAAACC+TGG | 0.527818 | 8.4:-57255749 | MS.gene59742:CDS |
| GGATGCCCTAAACATTAAGT+TGG | 0.528997 | 8.4:-57256639 | MS.gene59742:CDS |
| CTCTCCATATGTGCATCAGT+TGG | 0.531103 | 8.4:+57256615 | None:intergenic |
| AACTCAAAGAGGCACAAGAA+AGG | 0.544668 | 8.4:-57257051 | MS.gene59742:CDS |
| CCAAAATCAAGTAAATGAAA+GGG | 0.546900 | 8.4:+57256437 | None:intergenic |
| TTCGATTCCTAGGATCCAAA+GGG | 0.548512 | 8.4:-57257008 | MS.gene59742:CDS |
| TTTCGATTCCTAGGATCCAA+AGG | 0.556072 | 8.4:-57257009 | MS.gene59742:CDS |
| TGAATCCAAACGACTTGGAA+TGG | 0.561012 | 8.4:-57256486 | MS.gene59742:CDS |
| AGTTATCTACTATGGATGAA+AGG | 0.569581 | 8.4:-57257081 | None:intergenic |
| AAATAAATGGAAGACATGGT+TGG | 0.569744 | 8.4:-57255844 | MS.gene59742:CDS |
| ATGGGTGAATCCAAACGACT+TGG | 0.575258 | 8.4:-57256491 | MS.gene59742:CDS |
| CACTCATTTCAATACACCAT+GGG | 0.583087 | 8.4:-57255808 | MS.gene59742:CDS |
| ATAGGCTACTGCATTTGTGT+AGG | 0.583955 | 8.4:+57255914 | None:intergenic |
| TGTTTAGGGCATCCGGACGT+TGG | 0.585198 | 8.4:+57256648 | None:intergenic |
| AAATGTTGATGACCAACGTC+CGG | 0.586795 | 8.4:-57256660 | MS.gene59742:CDS |
| AAGGCTTTCGCAACTCAAAG+AGG | 0.638415 | 8.4:-57257062 | MS.gene59742:CDS |
| ACACTCATTTCAATACACCA+TGG | 0.648316 | 8.4:-57255809 | MS.gene59742:CDS |
| AACTTTGAAGAACAATCAGA+AGG | 0.650464 | 8.4:-57256395 | MS.gene59742:CDS |
| TTCATTGGTTGACAATGCAG+AGG | 0.655855 | 8.4:-57256057 | MS.gene59742:CDS |
| TAGCAAATAAGACAGATGTG+TGG | 0.692591 | 8.4:-57256250 | MS.gene59742:CDS |
| AAGAACTAAAGCCAGTACTG+CGG | 0.721960 | 8.4:+57255767 | None:intergenic |
| TGTGAAGAATATGTTCACTG+AGG | 0.803057 | 8.4:-57256762 | MS.gene59742:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAAGCATTACAAAAATAAA+TGG | - | chr8.4:57256929-57256948 | MS.gene59742:CDS | 15.0% |
| !!! | AATTAACAATTCTTTTGATA+TGG | - | chr8.4:57256276-57256295 | MS.gene59742:CDS | 15.0% |
| !!! | ATTAACAATTCTTTTGATAT+GGG | - | chr8.4:57256277-57256296 | MS.gene59742:CDS | 15.0% |
| !! | ACGTCATTGAATAAAAAAAT+AGG | + | chr8.4:57256616-57256635 | None:intergenic | 20.0% |
| !! | ATTAAGTTTCGAAAAACATA+AGG | + | chr8.4:57256643-57256662 | None:intergenic | 20.0% |
| ! | AAACCAAAAAGATAGTAATG+AGG | - | chr8.4:57256444-57256463 | MS.gene59742:CDS | 25.0% |
| ! | ACAAAAATAAATGGAAGACA+TGG | - | chr8.4:57256938-57256957 | MS.gene59742:CDS | 25.0% |
| ! | ACATTTCGTTCAAATCTAAA+TGG | - | chr8.4:57256569-57256588 | MS.gene59742:CDS | 25.0% |
| ! | ACCAAAATCAAGTAAATGAA+AGG | + | chr8.4:57256353-57256372 | None:intergenic | 25.0% |
| ! | CCAAAATCAAGTAAATGAAA+GGG | + | chr8.4:57256352-57256371 | None:intergenic | 25.0% |
| ! | TTCAAATCTAAATGGTTTAG+TGG | - | chr8.4:57256577-57256596 | MS.gene59742:CDS | 25.0% |
| !! | AGAATCTCAAGCATTTTTAT+TGG | + | chr8.4:57256223-57256242 | None:intergenic | 25.0% |
| !! | AGGAAGAAATTGATTCTAAT+TGG | - | chr8.4:57256464-57256483 | MS.gene59742:CDS | 25.0% |
| !! | GAGAATATATTTCTTGCTAT+AGG | + | chr8.4:57256893-57256912 | None:intergenic | 25.0% |
| !! | TTGTTGCAATACATTTTTGT+TGG | + | chr8.4:57256850-57256869 | None:intergenic | 25.0% |
| !!! | TCAAAATTTGTTGCTGATTA+TGG | - | chr8.4:57255929-57255948 | MS.gene59742:CDS | 25.0% |
| !!! | TCATTTTTCCGTAAAAACAA+TGG | + | chr8.4:57255806-57255825 | None:intergenic | 25.0% |
| !!! | TGGTTTTGAGTTGTATTTTT+TGG | + | chr8.4:57256430-57256449 | None:intergenic | 25.0% |
| AAATAAATGGAAGACATGGT+TGG | - | chr8.4:57256942-57256961 | MS.gene59742:CDS | 30.0% | |
| AAGAATGAAAGTCTGAAAGA+AGG | - | chr8.4:57255881-57255900 | MS.gene59742:CDS | 30.0% | |
| ACACCAAAGATCATATCATT+TGG | - | chr8.4:57255845-57255864 | MS.gene59742:CDS | 30.0% | |
| ATAATCGAATTTCGATTCCT+AGG | - | chr8.4:57255768-57255787 | MS.gene59742:CDS | 30.0% | |
| ATACGAGATCTAAAAACAGT+AGG | - | chr8.4:57256502-57256521 | MS.gene59742:CDS | 30.0% | |
| CTTTACCTTTATGGATTCAT+TGG | - | chr8.4:57256714-57256733 | MS.gene59742:CDS | 30.0% | |
| CTTTCATTCTTGTGATGAAT+AGG | + | chr8.4:57255872-57255891 | None:intergenic | 30.0% | |
| GCAAAACTCTTCAATGATTT+GGG | - | chr8.4:57256814-57256833 | MS.gene59742:CDS | 30.0% | |
| GGAATCTTTCTTAGGATTTA+CGG | + | chr8.4:57257067-57257086 | None:intergenic | 30.0% | |
| TCATAGTTGTAATGAACATC+TGG | + | chr8.4:57256682-57256701 | None:intergenic | 30.0% | |
| TCTGATTGTTCTTCAAAGTT+GGG | + | chr8.4:57256391-57256410 | None:intergenic | 30.0% | |
| TGGAATGGTAATAGTCAAAT+TGG | - | chr8.4:57256315-57256334 | MS.gene59742:CDS | 30.0% | |
| TTCTGATTGTTCTTCAAAGT+TGG | + | chr8.4:57256392-57256411 | None:intergenic | 30.0% | |
| TTGGTCCAACTTAATGTTTA+GGG | + | chr8.4:57256155-57256174 | None:intergenic | 30.0% | |
| ! | AACTTTGAAGAACAATCAGA+AGG | - | chr8.4:57256391-57256410 | MS.gene59742:CDS | 30.0% |
| ! | CCCTTTCATTTACTTGATTT+TGG | - | chr8.4:57256349-57256368 | MS.gene59742:CDS | 30.0% |
| ! | CTTCCTCATTACTATCTTTT+TGG | + | chr8.4:57256450-57256469 | None:intergenic | 30.0% |
| !! | GAAGAAATGGGTATTGATTT+GGG | - | chr8.4:57256247-57256266 | MS.gene59742:CDS | 30.0% |
| !!! | AAAGGGTTCCATTGTTTTTA+CGG | - | chr8.4:57255795-57255814 | MS.gene59742:CDS | 30.0% |
| !!! | ATGGAGAGATACTTTTTTGT+TGG | - | chr8.4:57256186-57256205 | MS.gene59742:CDS | 30.0% |
| AAAAACAGTAGGAATTCGAG+TGG | - | chr8.4:57256513-57256532 | MS.gene59742:CDS | 35.0% | |
| ACACTCATTTCAATACACCA+TGG | - | chr8.4:57256977-57256996 | MS.gene59742:CDS | 35.0% | |
| ATAAGACAGATGTGTGGAAA+TGG | - | chr8.4:57256542-57256561 | MS.gene59742:CDS | 35.0% | |
| ATGTTCTTTGCTGCACTTTA+TGG | - | chr8.4:57256102-57256121 | MS.gene59742:CDS | 35.0% | |
| CACTCATTTCAATACACCAT+GGG | - | chr8.4:57256978-57256997 | MS.gene59742:CDS | 35.0% | |
| GAGATTCTATGCGAAGAAAT+GGG | - | chr8.4:57256235-57256254 | MS.gene59742:CDS | 35.0% | |
| GGACCAAATGATATGATCTT+TGG | + | chr8.4:57255851-57255870 | None:intergenic | 35.0% | |
| GGCAAAACTCTTCAATGATT+TGG | - | chr8.4:57256813-57256832 | MS.gene59742:CDS | 35.0% | |
| GTAAAAACAATGGAACCCTT+TGG | + | chr8.4:57255796-57255815 | None:intergenic | 35.0% | |
| GTCAACCAATGAATCCATAA+AGG | + | chr8.4:57256722-57256741 | None:intergenic | 35.0% | |
| GTTGGTCCAACTTAATGTTT+AGG | + | chr8.4:57256156-57256175 | None:intergenic | 35.0% | |
| GTTTGCTTGGATATTGTTTG+TGG | - | chr8.4:57256075-57256094 | MS.gene59742:CDS | 35.0% | |
| TAGCAAATAAGACAGATGTG+TGG | - | chr8.4:57256536-57256555 | MS.gene59742:CDS | 35.0% | |
| TATTACCATTCCAAGTCGTT+TGG | + | chr8.4:57256308-57256327 | None:intergenic | 35.0% | |
| TCAAGTAAATGAAAGGGCTT+AGG | + | chr8.4:57256346-57256365 | None:intergenic | 35.0% | |
| TGAGATTCTATGCGAAGAAA+TGG | - | chr8.4:57256234-57256253 | MS.gene59742:CDS | 35.0% | |
| TGTGAAGAATATGTTCACTG+AGG | - | chr8.4:57256024-57256043 | MS.gene59742:CDS | 35.0% | |
| TTCTTACTTTCATCCAAACC+TGG | - | chr8.4:57257037-57257056 | MS.gene59742:CDS | 35.0% | |
| TTGCTCGTTCTTTACCTTTA+TGG | - | chr8.4:57256705-57256724 | MS.gene59742:CDS | 35.0% | |
| ! | AACTTAATGTTTAGGGCATC+CGG | + | chr8.4:57256148-57256167 | None:intergenic | 35.0% |
| ! | ACAATGATAACGAGTTTGCT+TGG | - | chr8.4:57256062-57256081 | MS.gene59742:CDS | 35.0% |
| ! | GGTGTATTTCAAAACTTGCT+TGG | - | chr8.4:57256775-57256794 | MS.gene59742:CDS | 35.0% |
| !! | CGAAGAAATGGGTATTGATT+TGG | - | chr8.4:57256246-57256265 | MS.gene59742:CDS | 35.0% |
| AAAAAGGCAATCATAGCCCA+TGG | + | chr8.4:57256997-57257016 | None:intergenic | 40.0% | |
| AAGAACTAAAGCCAGTACTG+CGG | + | chr8.4:57257022-57257041 | None:intergenic | 40.0% | |
| ATAGGCTACTGCATTTGTGT+AGG | + | chr8.4:57256875-57256894 | None:intergenic | 40.0% | |
| CTTAGGATTTACGGTAAACC+AGG | + | chr8.4:57257058-57257077 | None:intergenic | 40.0% | |
| GATTTACGGTAAACCAGGTT+TGG | + | chr8.4:57257053-57257072 | None:intergenic | 40.0% | |
| GCTTGGATATTGTTTGTGGA+TGG | - | chr8.4:57256079-57256098 | MS.gene59742:CDS | 40.0% | |
| GGATGCCCTAAACATTAAGT+TGG | - | chr8.4:57256147-57256166 | MS.gene59742:CDS | 40.0% | |
| GTAAAAGAGCTTAGATCAGC+TGG | - | chr8.4:57256754-57256773 | MS.gene59742:CDS | 40.0% | |
| TTCACTGAGGATGCAATTGA+AGG | - | chr8.4:57256037-57256056 | MS.gene59742:CDS | 40.0% | |
| TTCGATTCCTAGGATCCAAA+GGG | - | chr8.4:57255778-57255797 | MS.gene59742:CDS | 40.0% | |
| TTTCGATTCCTAGGATCCAA+AGG | - | chr8.4:57255777-57255796 | MS.gene59742:CDS | 40.0% | |
| ! | AACTCAAAGAGGCACAAGAA+AGG | - | chr8.4:57255735-57255754 | MS.gene59742:CDS | 40.0% |
| ! | TGAATCCAAACGACTTGGAA+TGG | - | chr8.4:57256300-57256319 | MS.gene59742:CDS | 40.0% |
| ! | TTCATTGGTTGACAATGCAG+AGG | - | chr8.4:57256729-57256748 | MS.gene59742:CDS | 40.0% |
| !! | AAATGTTGATGACCAACGTC+CGG | - | chr8.4:57256126-57256145 | MS.gene59742:CDS | 40.0% |
| !! | GAGAGCATTACAAGCTTCTA+TGG | - | chr8.4:57255903-57255922 | MS.gene59742:CDS | 40.0% |
| AAGGCTTTCGCAACTCAAAG+AGG | - | chr8.4:57255724-57255743 | MS.gene59742:CDS | 45.0% | |
| AATGGAACCCTTTGGATCCT+AGG | + | chr8.4:57255788-57255807 | None:intergenic | 45.0% | |
| ATGGGTGAATCCAAACGACT+TGG | - | chr8.4:57256295-57256314 | MS.gene59742:CDS | 45.0% | |
| CTCTCCATATGTGCATCAGT+TGG | + | chr8.4:57256174-57256193 | None:intergenic | 45.0% | |
| TGGACCAACTGATGCACATA+TGG | - | chr8.4:57256167-57256186 | MS.gene59742:CDS | 45.0% | |
| AGCCAGTACTGCGGCTAAAA+AGG | + | chr8.4:57257013-57257032 | None:intergenic | 50.0% | |
| GCTTGGAAGCGACGAAGAAT+TGG | - | chr8.4:57256792-57256811 | MS.gene59742:CDS | 50.0% | |
| !! | TGCCTTTTTAGCCGCAGTAC+TGG | - | chr8.4:57257008-57257027 | MS.gene59742:CDS | 50.0% |
| ! | TGTTTAGGGCATCCGGACGT+TGG | + | chr8.4:57256141-57256160 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 57255716 | 57257092 | 57255716 | ID=MS.gene59742 |
| chr8.4 | mRNA | 57255716 | 57257092 | 57255716 | ID=MS.gene59742.t1;Parent=MS.gene59742 |
| chr8.4 | exon | 57255716 | 57257092 | 57255716 | ID=MS.gene59742.t1.exon1;Parent=MS.gene59742.t1 |
| chr8.4 | CDS | 57255716 | 57257092 | 57255716 | ID=cds.MS.gene59742.t1;Parent=MS.gene59742.t1 |
| Gene Sequence |
| Protein sequence |
