AlfalfaGEDB | MS.gene60408

M. sativa cultivar XinJiangDaYe / MS.gene60408

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515 GO:0046983
PFAM: PF00010.29

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60408.t1	KEH16287.1	83.2	339	27	4	2	334	7	321	7.90E-139	503.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60408.t1	Q1PF16	40.6	187	99	4	152	327	108	293	6.3e-28	126.3

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60408.t1	A0A072TFY6	83.2	339	27	4	2	334	7	321	5.7e-139	503.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene60408.t1	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene04921	MS.gene60408	0.802353	5.84E-49	-1.69E-46
MS.gene052606	MS.gene60408	0.803944	2.74E-49	-1.69E-46
MS.gene05589	MS.gene60408	-0.81278	3.57E-51	-1.69E-46
MS.gene056590	MS.gene60408	0.80988	1.52E-50	-1.69E-46
MS.gene056591	MS.gene60408	0.812614	3.88E-51	-1.69E-46
MS.gene056694	MS.gene60408	0.824984	6.00E-54	-1.69E-46
MS.gene056697	MS.gene60408	0.814591	1.43E-51	-1.69E-46
MS.gene061044	MS.gene60408	0.822389	2.43E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60408.t1	MTR_4g098035	83.186	339	27	4	2	334	28	342	0.0	552
MS.gene60408.t1	MTR_0246s0050	83.186	339	27	4	2	334	7	321	0.0	551
MS.gene60408.t1	MTR_0246s0020	52.316	367	125	13	2	334	29	379	2.60e-99	298
MS.gene60408.t1	MTR_6g047570	49.858	353	113	15	2	334	28	336	6.18e-87	265
MS.gene60408.t1	MTR_0246s0020	49.304	359	130	14	2	326	29	369	2.87e-84	259
MS.gene60408.t1	MTR_0011s0210	47.025	353	147	13	2	334	28	360	3.77e-84	258
MS.gene60408.t1	MTR_0011s0260	47.159	352	146	13	2	333	28	359	5.41e-83	256
MS.gene60408.t1	MTR_4g066380	47.605	334	137	8	2	333	28	325	6.02e-82	252
MS.gene60408.t1	MTR_4g066460	48.665	337	133	12	2	334	29	329	9.74e-82	251
MS.gene60408.t1	MTR_4g067010	47.619	336	126	14	2	332	29	319	5.80e-79	244
MS.gene60408.t1	MTR_0250s0040	50.185	271	94	10	74	334	26	265	1.48e-69	218
MS.gene60408.t1	MTR_5g014520	42.262	336	152	8	5	333	33	333	6.02e-59	193
MS.gene60408.t1	MTR_5g014560	50.980	204	85	5	143	333	106	307	1.63e-54	181
MS.gene60408.t1	MTR_6g047550	48.760	242	86	12	2	232	28	242	5.08e-48	163
MS.gene60408.t1	MTR_2g010450	36.275	306	160	8	50	332	60	353	6.55e-45	157
MS.gene60408.t1	MTR_5g014600	38.077	260	125	4	97	329	84	334	1.48e-44	155
MS.gene60408.t1	MTR_4g009540	38.281	256	129	7	93	330	87	331	1.32e-40	145
MS.gene60408.t1	MTR_4g097940	40.984	183	100	3	148	324	140	320	1.96e-38	139
MS.gene60408.t1	MTR_4g097920	40.984	183	100	3	148	324	125	305	6.54e-38	137
MS.gene60408.t1	MTR_4g097920	40.984	183	100	3	148	324	140	320	7.95e-38	137
MS.gene60408.t1	MTR_4g092700	39.691	194	107	2	147	332	142	333	2.86e-36	134
MS.gene60408.t1	MTR_5g014640	31.633	294	180	6	54	329	33	323	5.44e-35	130
MS.gene60408.t1	MTR_8g009020	34.483	261	130	8	84	325	80	318	8.06e-35	130
MS.gene60408.t1	MTR_4g097950	40.625	192	93	2	157	331	64	251	9.80e-35	127
MS.gene60408.t1	MTR_2g104560	37.912	182	105	2	156	331	47	226	3.02e-34	125
MS.gene60408.t1	MTR_4g009110	40.394	203	102	5	145	330	150	350	8.45e-34	127
MS.gene60408.t1	MTR_4g009540	37.872	235	117	7	93	309	87	310	9.19e-34	126
MS.gene60408.t1	MTR_2g104490	37.500	216	115	8	119	324	97	302	1.96e-32	123
MS.gene60408.t1	MTR_2g104550	32.296	257	145	6	89	333	76	315	2.59e-32	122
MS.gene60408.t1	MTR_2g104500	35.780	218	117	6	128	330	63	272	4.58e-29	113
MS.gene60408.t1	MTR_7g080780	28.125	288	191	6	47	330	41	316	9.85e-29	113
MS.gene60408.t1	MTR_7g080780	27.972	286	190	5	49	330	56	329	1.24e-28	113
MS.gene60408.t1	MTR_2g104650	35.616	219	126	7	120	328	54	267	1.54e-27	109
MS.gene60408.t1	MTR_4g009510	38.411	151	89	2	177	327	56	202	5.84e-27	105
MS.gene60408.t1	MTR_8g009010	30.888	259	128	7	76	325	51	267	8.61e-27	108
MS.gene60408.t1	MTR_2g104530	32.701	211	126	4	128	325	62	269	2.37e-26	106
MS.gene60408.t1	MTR_2g104490	36.318	201	108	8	119	309	97	287	5.84e-26	105
MS.gene60408.t1	MTR_4g009420	40.288	139	71	2	189	327	68	194	3.46e-25	100
MS.gene60408.t1	MTR_7g451650	38.849	139	81	2	189	327	60	194	1.25e-24	99.4
MS.gene60408.t1	MTR_8g069740	36.145	166	96	4	152	308	101	265	4.45e-24	99.8
MS.gene60408.t1	MTR_7g050350	37.778	135	80	2	193	327	2	132	2.78e-23	94.0
MS.gene60408.t1	MTR_7g451650	37.778	135	80	2	193	327	2	132	2.78e-23	94.0
MS.gene60408.t1	MTR_2g104590	32.461	191	89	5	152	328	58	222	4.31e-21	90.5
MS.gene60408.t1	MTR_5g014550	43.902	123	59	3	193	307	54	174	1.97e-19	85.1
MS.gene60408.t1	MTR_4g009420	37.903	124	65	2	186	309	65	176	6.06e-18	81.3
MS.gene60408.t1	MTR_7g451650	37.190	121	72	2	189	309	60	176	1.05e-17	80.5
MS.gene60408.t1	MTR_7g117670	31.373	153	96	4	163	311	402	549	5.38e-15	76.3
MS.gene60408.t1	MTR_1g072320	31.690	142	83	2	163	290	470	611	9.23e-14	72.4
MS.gene60408.t1	MTR_8g067280	32.168	143	80	3	163	288	462	604	9.60e-14	72.4
MS.gene60408.t1	MTR_8g027495	30.061	163	103	5	163	321	458	613	1.39e-12	68.9
MS.gene60408.t1	MTR_5g030430	27.684	177	95	5	139	288	465	635	1.69e-11	65.5
MS.gene60408.t1	MTR_8g024790	30.882	136	79	6	163	286	291	423	2.08e-11	65.1
MS.gene60408.t1	MTR_3g498695	27.083	192	120	5	138	312	274	462	6.10e-11	63.5
MS.gene60408.t1	MTR_3g498695	26.042	192	123	4	138	312	274	463	8.38e-11	63.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60408.t1	AT2G22760	40.314	191	100	5	151	329	107	295	1.31e-37	136
MS.gene60408.t1	AT2G22770	33.473	239	131	3	111	327	85	317	8.79e-37	135
MS.gene60408.t1	AT4G37850	35.743	249	140	6	96	332	88	328	4.09e-36	133
MS.gene60408.t1	AT4G37850	35.743	249	140	6	96	332	88	328	4.09e-36	133
MS.gene60408.t1	AT2G22760	40.588	170	88	4	151	309	107	274	1.37e-32	122
MS.gene60408.t1	AT2G22750	38.974	195	106	5	149	331	111	304	1.37e-30	118
MS.gene60408.t1	AT2G22750	38.974	195	106	5	149	331	135	328	1.47e-30	118
MS.gene60408.t1	AT2G22750	38.974	195	106	5	149	331	139	332	1.54e-30	118
MS.gene60408.t1	AT2G22750	38.974	195	105	6	149	331	111	303	1.80e-29	115
MS.gene60408.t1	AT2G22750	38.974	195	105	6	149	331	139	331	2.35e-29	115
MS.gene60408.t1	AT5G46760	30.899	178	96	7	163	313	415	592	7.15e-15	75.9
MS.gene60408.t1	AT2G46510	37.634	93	53	2	163	252	395	485	1.07e-13	72.4
MS.gene60408.t1	AT4G17880	33.103	145	84	3	163	294	416	560	1.46e-13	72.0
MS.gene60408.t1	AT4G09820	33.043	115	63	2	164	278	364	464	9.37e-13	69.3
MS.gene60408.t1	AT1G32640	26.007	273	171	9	43	288	316	584	1.26e-12	68.9
MS.gene60408.t1	AT1G01260	28.571	126	74	2	142	267	412	521	2.79e-12	68.2
MS.gene60408.t1	AT1G01260	28.571	126	74	2	142	267	412	521	2.79e-12	68.2
MS.gene60408.t1	AT1G01260	28.571	126	74	2	142	267	412	521	2.79e-12	68.2
MS.gene60408.t1	AT1G63650	28.378	148	82	2	163	286	405	552	6.40e-11	63.9
MS.gene60408.t1	AT1G63650	28.378	148	82	2	163	286	405	552	6.40e-11	63.9
MS.gene60408.t1	AT1G63650	28.378	148	82	2	163	286	405	552	6.40e-11	63.9
MS.gene60408.t1	AT4G16430	30.968	155	90	5	73	219	231	376	6.51e-11	63.5
MS.gene60408.t1	AT5G41315	28.105	153	81	4	163	286	446	598	7.39e-11	63.5
MS.gene60408.t1	AT5G41315	28.105	153	81	4	163	286	441	593	7.43e-11	63.5
MS.gene60408.t1	AT5G41315	28.105	153	81	4	163	286	449	601	7.89e-11	63.5
MS.gene60408.t1	AT3G06120	28.141	199	86	8	163	320	2	184	8.86e-11	61.2
MS.gene60408.t1	AT1G12860	28.966	145	91	4	164	296	268	412	9.58e-11	63.2
MS.gene60408.t1	AT3G61950	29.197	137	87	4	163	290	128	263	9.90e-11	62.4

Find 53 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCAGTGTCATTGCCCAATT+TGG	0.159846	8.3:+22904889	MS.gene60408:CDS
AAAACGTGTGTTATCCAAAT+TGG	0.202416	8.3:-22904903	None:intergenic
CCAAACCCAACAGATTTATA+CGG	0.283498	8.3:+22904839	MS.gene60408:CDS
CAAGAAGTCCATCATGCTTT+TGG	0.296888	8.3:-22905702	None:intergenic
CGTATAAATCTGTTGGGTTT+GGG	0.304768	8.3:-22904838	None:intergenic
AGTTACTTCTTACCTTCTTT+AGG	0.329290	8.3:-22905090	None:intergenic
ACAATAGTGGTCTGGTTTGT+TGG	0.341236	8.3:-22905596	None:intergenic
CCGTATAAATCTGTTGGGTT+TGG	0.355612	8.3:-22904839	None:intergenic
AATTTGTTGTGAAAAGCTTT+TGG	0.355869	8.3:-22904765	None:intergenic
GGCTAGTGCCATTGCCTTTG+AGG	0.361619	8.3:+22904601	MS.gene60408:CDS
ATGCTGCCATGCGTAGATTC+TGG	0.364951	8.3:-22907282	None:intergenic
AACGCTCTTTAAGCTCTTTG+AGG	0.388426	8.3:-22905513	None:intergenic
CTTCTTACCTTCTTTAGGTC+AGG	0.391987	8.3:-22905085	None:intergenic
TCATGTAAAAGATCACATTA+TGG	0.398549	8.3:+22905003	MS.gene60408:CDS
TTGGTGATGCTACTGCTATC+AGG	0.398588	8.3:+22905632	MS.gene60408:CDS
CACTTTCAGTTTCACAATAG+TGG	0.411282	8.3:-22905609	None:intergenic
AGGGTAGAAGCAAAAGTGTC+AGG	0.446583	8.3:+22905652	MS.gene60408:CDS
TCAGTTTCACAATAGTGGTC+TGG	0.454097	8.3:-22905604	None:intergenic
AAACGTGTGTTATCCAAATT+GGG	0.461557	8.3:-22904902	None:intergenic
TCAAACCCGTATAAATCTGT+TGG	0.488589	8.3:-22904845	None:intergenic
CAAACCCGTATAAATCTGTT+GGG	0.491045	8.3:-22904844	None:intergenic
GAGTTCTGCAAGAGCAATGA+AGG	0.495896	8.3:-22905059	None:intergenic
ATCACAATCTAAGAGGTCTT+CGG	0.516297	8.3:+22904979	MS.gene60408:CDS
CAAACCCAACAGATTTATAC+GGG	0.531352	8.3:+22904840	MS.gene60408:CDS
CTAAAACATCACAATCTAAG+AGG	0.533656	8.3:+22904972	MS.gene60408:CDS
AATCTCCAAAGGCTAAAATA+CGG	0.534934	8.3:-22905774	None:intergenic
GGGTTTGGGTTGTCAAAAGA+CGG	0.535552	8.3:-22904824	None:intergenic
ACTCATTCCTGACCTAAAGA+AGG	0.552914	8.3:+22905078	MS.gene60408:CDS
GAATGTTTCGCTTAGAGTGG+TGG	0.560814	8.3:-22904654	None:intergenic
AGTCTCTCTTTGTTCCTCAA+AGG	0.562733	8.3:-22904615	None:intergenic
TGAGATGACTGAAAAGAGGA+TGG	0.564225	8.3:-22904797	None:intergenic
TCTTTGTTCCTCAAAGGCAA+TGG	0.566921	8.3:-22904609	None:intergenic
TGGTGATGCTACTGCTATCA+GGG	0.568737	8.3:+22905633	MS.gene60408:CDS
ATTGAATGTTTCGCTTAGAG+TGG	0.570806	8.3:-22904657	None:intergenic
ATCCACTGCCAAAAGCATGA+TGG	0.574310	8.3:+22905694	MS.gene60408:CDS
AATCTGAGATGACTGAAAAG+AGG	0.576642	8.3:-22904801	None:intergenic
ACTCTAAGCGAAACATTCAA+TGG	0.582474	8.3:+22904659	MS.gene60408:CDS
CAGCAATGATTGTAATGTCG+TGG	0.584079	8.3:-22905801	None:intergenic
GGGTAGAAGCAAAAGTGTCA+GGG	0.600504	8.3:+22905653	MS.gene60408:CDS
ATTGGGCAATGACACTGAAG+TGG	0.602756	8.3:-22904885	None:intergenic
AGAGTTTCTCTCACGTGACA+TGG	0.613188	8.3:+22904580	MS.gene60408:CDS
TGAATACATGTTATTTGACA+AGG	0.622791	8.3:+22904520	None:intergenic
TTGGGCAATGACACTGAAGT+GGG	0.628306	8.3:-22904884	None:intergenic
ATATGTTAATTAGAAGGACA+AGG	0.637697	8.3:+22905462	MS.gene60408:intron
CTGAAAAGAGGATGGAGATG+CGG	0.641434	8.3:-22904789	None:intergenic
CACAAAGGAAGATCAATCGA+TGG	0.644880	8.3:+22905567	MS.gene60408:CDS
GAACTTGCTTGATGCTGACG+TGG	0.647274	8.3:+22904553	MS.gene60408:CDS
CGACATTACAATCATTGCTG+AGG	0.658041	8.3:+22905804	MS.gene60408:CDS
GTCGTGGAAAGAATCTCCAA+AGG	0.662563	8.3:-22905785	None:intergenic
TATTGTGAAACTGAAAGTGT+TGG	0.666038	8.3:+22905613	MS.gene60408:CDS
AGAAAACGAAGAGAGAAACT+CGG	0.671233	8.3:+22905031	MS.gene60408:CDS
ACTCGTCCAGAATCTACGCA+TGG	0.674291	8.3:+22907276	MS.gene60408:CDS
AAAGGAAGATCAATCGATGG+TGG	0.686344	8.3:+22905570	MS.gene60408:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATAATTTAAGAAAATTAAAG+TGG	-	chr8.3:22905406-22905425	None:intergenic	10.0%
!!	CTATTATATATAAAAACAAA+TGG	-	chr8.3:22905278-22905297	None:intergenic	10.0%
!!	TATATAATAGTAAAAGAATA+TGG	+	chr8.3:22905288-22905307	MS.gene60408:intron	10.0%
!!	ACATCAAAATATTTAATGAT+TGG	-	chr8.3:22906195-22906214	None:intergenic	15.0%
!!	TCTTAATAAATAAATCACTA+TGG	+	chr8.3:22906942-22906961	MS.gene60408:intron	15.0%
!!	TGTAAAATATGTTAATTAGA+AGG	+	chr8.3:22905456-22905475	MS.gene60408:intron	15.0%
!!	TTATTAGATGAAATTCATAT+AGG	-	chr8.3:22906870-22906889	None:intergenic	15.0%
!!!	CACTCATTTTATAAAAAATT+AGG	+	chr8.3:22906805-22906824	MS.gene60408:intron	15.0%
!!!	CTTCTTTTTTATCTTTTTAT+TGG	+	chr8.3:22906082-22906101	MS.gene60408:intron	15.0%
!!!	GTCTAGAAATTTTTATATAT+GGG	-	chr8.3:22906969-22906988	None:intergenic	15.0%
!!!	TCTAGAAATTTTTATATATG+GGG	-	chr8.3:22906968-22906987	None:intergenic	15.0%
!!	ACAAAATAAACTCTATGATT+TGG	-	chr8.3:22905957-22905976	None:intergenic	20.0%
!!	ATTATTCTCTTTGTCAAAAA+AGG	+	chr8.3:22906384-22906403	MS.gene60408:intron	20.0%
!!	TAAAAAGTAATCAAGCTTAT+GGG	-	chr8.3:22906171-22906190	None:intergenic	20.0%
!!	TAACAATCAATACAAATAAG+TGG	-	chr8.3:22907204-22907223	None:intergenic	20.0%
!!	TAATACATGGTTAAAGATAT+AGG	-	chr8.3:22905192-22905211	None:intergenic	20.0%
!!	TAGAATCTATTCCTAAAATA+TGG	-	chr8.3:22906832-22906851	None:intergenic	20.0%
!!	TATCATGAACTTAAATTGTA+GGG	+	chr8.3:22906490-22906509	MS.gene60408:intron	20.0%
!!	TTAAAAAGTAATCAAGCTTA+TGG	-	chr8.3:22906172-22906191	None:intergenic	20.0%
!!	TTATTCTCTTTGTCAAAAAA+GGG	+	chr8.3:22906385-22906404	MS.gene60408:intron	20.0%
!!!	AAAAATTAGGTCCATATTTT+AGG	+	chr8.3:22906818-22906837	MS.gene60408:intron	20.0%
!!!	CAATTTTAGAAGAAGAAAAA+GGG	+	chr8.3:22905539-22905558	MS.gene60408:CDS	20.0%
!!!	CATTCTCTTTAAAAAAACAA+TGG	-	chr8.3:22906692-22906711	None:intergenic	20.0%
!!!	CTTAAATAATGAAAACGTTT+TGG	-	chr8.3:22906352-22906371	None:intergenic	20.0%
!!!	GGTCTAGAAATTTTTATATA+TGG	-	chr8.3:22906970-22906989	None:intergenic	20.0%
!!!	TCAATTTTAGAAGAAGAAAA+AGG	+	chr8.3:22905538-22905557	MS.gene60408:CDS	20.0%
!!!	TCTATTATTGTTTTACAGAT+TGG	+	chr8.3:22907226-22907245	MS.gene60408:intron	20.0%
!!!	TGAACTTTTCATAGTTTATA+TGG	+	chr8.3:22906561-22906580	MS.gene60408:intron	20.0%
!!!	TTATAAGTTTTGCTAATACA+TGG	-	chr8.3:22905205-22905224	None:intergenic	20.0%
!!!	TTTTTTGACAAAGAGAATAA+TGG	-	chr8.3:22906385-22906404	None:intergenic	20.0%
!	AAATATAAATGAGAGAACAG+TGG	-	chr8.3:22906763-22906782	None:intergenic	25.0%
!	AAGATGATGTAGAGAATAAA+TGG	-	chr8.3:22905879-22905898	None:intergenic	25.0%
!	ATATGTTAATTAGAAGGACA+AGG	+	chr8.3:22905462-22905481	MS.gene60408:intron	25.0%
!	ATCAAAAAATTTCAGCTTCT+TGG	-	chr8.3:22904729-22904748	None:intergenic	25.0%
!	ATGTTTAAGCGATAAAATGA+TGG	-	chr8.3:22906718-22906737	None:intergenic	25.0%
!	GTATCATGAACTTAAATTGT+AGG	+	chr8.3:22906489-22906508	MS.gene60408:intron	25.0%
!	TATAGGTTAAAGATGTGTAT+TGG	-	chr8.3:22905175-22905194	None:intergenic	25.0%
!	TCATGTAAAAGATCACATTA+TGG	+	chr8.3:22905003-22905022	MS.gene60408:CDS	25.0%
!	TGAATGAGAAAGTTGTTTAA+TGG	+	chr8.3:22907154-22907173	MS.gene60408:intron	25.0%
!!	ATAACTTTTCTCTTTTTGTG+TGG	+	chr8.3:22906916-22906935	MS.gene60408:intron	25.0%
!!	TAATGTTAGATTTGTTTCGA+CGG	-	chr8.3:22905250-22905269	None:intergenic	25.0%
!!!	AATTTGTTGTGAAAAGCTTT+TGG	-	chr8.3:22904768-22904787	None:intergenic	25.0%
!!!	ATTTTTTGAAGACTCAATTG+AGG	-	chr8.3:22904939-22904958	None:intergenic	25.0%
!!!	TTTTCATAAGTTGAGATTGT+GGG	-	chr8.3:22906288-22906307	None:intergenic	25.0%
!!!	TTTTTTGAAGACTCAATTGA+GGG	-	chr8.3:22904938-22904957	None:intergenic	25.0%
	AAAAATTTCAGCTTCTTGGT+TGG	-	chr8.3:22904725-22904744	None:intergenic	30.0%
	AAAACGTGTGTTATCCAAAT+TGG	-	chr8.3:22904906-22904925	None:intergenic	30.0%
	AAACGTGTGTTATCCAAATT+GGG	-	chr8.3:22904905-22904924	None:intergenic	30.0%
	AATCTCCAAAGGCTAAAATA+CGG	-	chr8.3:22905777-22905796	None:intergenic	30.0%
	AGGGCTTAATACAAGTAAAA+AGG	+	chr8.3:22906509-22906528	MS.gene60408:intron	30.0%
	AGTTACTTCTTACCTTCTTT+AGG	-	chr8.3:22905093-22905112	None:intergenic	30.0%
	ATGGTTGTATAATTTCTCTC+TGG	+	chr8.3:22907173-22907192	MS.gene60408:intron	30.0%
	CTAAAACATCACAATCTAAG+AGG	+	chr8.3:22904972-22904991	MS.gene60408:CDS	30.0%
	GGGCTTAATACAAGTAAAAA+GGG	+	chr8.3:22906510-22906529	MS.gene60408:intron	30.0%
	GTCTTCAAAAAATCAGAACT+TGG	+	chr8.3:22904946-22904965	MS.gene60408:CDS	30.0%
!	AAAGCTTGCAGTTTGTATTT+TGG	-	chr8.3:22906454-22906473	None:intergenic	30.0%
!	AAGCTTGCAGTTTGTATTTT+GGG	-	chr8.3:22906453-22906472	None:intergenic	30.0%
!	AATAGAGAGAAGTATGAACA+TGG	+	chr8.3:22906113-22906132	MS.gene60408:intron	30.0%
!	ATAGAGAGAAGTATGAACAT+GGG	+	chr8.3:22906114-22906133	MS.gene60408:intron	30.0%
!	TATTGTGAAACTGAAAGTGT+TGG	+	chr8.3:22905613-22905632	MS.gene60408:CDS	30.0%
!	TTTCATAAGTTGAGATTGTG+GGG	-	chr8.3:22906287-22906306	None:intergenic	30.0%
!!	ATTGTTTTACAGATTGGTGA+AGG	+	chr8.3:22907232-22907251	MS.gene60408:intron	30.0%
!!	ATTTTGATGTTTTGCTCTAG+AGG	+	chr8.3:22906205-22906224	MS.gene60408:intron	30.0%
!!	GTTTTCATAAGTTGAGATTG+TGG	-	chr8.3:22906289-22906308	None:intergenic	30.0%
!!!	AATAACCGTATTTTAGCCTT+TGG	+	chr8.3:22905769-22905788	MS.gene60408:CDS	30.0%
!!!	CGTTTGTTTTTACAAAAGTG+CGG	-	chr8.3:22907111-22907130	None:intergenic	30.0%
	ACTCTAAGCGAAACATTCAA+TGG	+	chr8.3:22904659-22904678	MS.gene60408:CDS	35.0%
	AGAAAAAGGGAAGAACACAA+AGG	+	chr8.3:22905552-22905571	MS.gene60408:CDS	35.0%
	AGAAAACGAAGAGAGAAACT+CGG	+	chr8.3:22905031-22905050	MS.gene60408:CDS	35.0%
	ATATCACGTCTAACATGCAT+TGG	-	chr8.3:22907043-22907062	None:intergenic	35.0%
	ATCACAATCTAAGAGGTCTT+CGG	+	chr8.3:22904979-22904998	MS.gene60408:CDS	35.0%
	ATGCATTGGGAAGTGAAATT+TGG	-	chr8.3:22907029-22907048	None:intergenic	35.0%
	ATTGAATGTTTCGCTTAGAG+TGG	-	chr8.3:22904660-22904679	None:intergenic	35.0%
	CAAACCCAACAGATTTATAC+GGG	+	chr8.3:22904840-22904859	MS.gene60408:CDS	35.0%
	CAAACCCGTATAAATCTGTT+GGG	-	chr8.3:22904847-22904866	None:intergenic	35.0%
	CACTTTCAGTTTCACAATAG+TGG	-	chr8.3:22905612-22905631	None:intergenic	35.0%
	CCAAACCCAACAGATTTATA+CGG	+	chr8.3:22904839-22904858	MS.gene60408:CDS	35.0%
	CGTATAAATCTGTTGGGTTT+GGG	-	chr8.3:22904841-22904860	None:intergenic	35.0%
	GACTAGTAAGACATAGTCAT+AGG	-	chr8.3:22905433-22905452	None:intergenic	35.0%
	GGCAACACAATATTTGAAAG+GGG	-	chr8.3:22906265-22906284	None:intergenic	35.0%
	GGGCAACACAATATTTGAAA+GGG	-	chr8.3:22906266-22906285	None:intergenic	35.0%
	TACAATCATTGCTGAGGTAA+AGG	+	chr8.3:22905810-22905829	MS.gene60408:intron	35.0%
	TATCACGTCTAACATGCATT+GGG	-	chr8.3:22907042-22907061	None:intergenic	35.0%
	TCAAACCCGTATAAATCTGT+TGG	-	chr8.3:22904848-22904867	None:intergenic	35.0%
	TCCAAACATGTCATTAGTGA+CGG	-	chr8.3:22906992-22907011	None:intergenic	35.0%
	TGACAAAGAGAATAATGGCA+TGG	-	chr8.3:22906380-22906399	None:intergenic	35.0%
!	AATCTGAGATGACTGAAAAG+AGG	-	chr8.3:22904804-22904823	None:intergenic	35.0%
!	ATTCTGGACGAGTTCTTTTA+AGG	-	chr8.3:22907269-22907288	None:intergenic	35.0%
!	CAGGGAAAAAGATGTTGATT+AGG	+	chr8.3:22905671-22905690	MS.gene60408:CDS	35.0%
!	TTCATAAGTTGAGATTGTGG+GGG	-	chr8.3:22906286-22906305	None:intergenic	35.0%
	AAAGGAAGATCAATCGATGG+TGG	+	chr8.3:22905570-22905589	MS.gene60408:CDS	40.0%
	AACGCTCTTTAAGCTCTTTG+AGG	-	chr8.3:22905516-22905535	None:intergenic	40.0%
	ACTCATTCCTGACCTAAAGA+AGG	+	chr8.3:22905078-22905097	MS.gene60408:CDS	40.0%
	AGTCTCTCTTTGTTCCTCAA+AGG	-	chr8.3:22904618-22904637	None:intergenic	40.0%
	ATCTGCTATAGTGACTGTGA+CGG	-	chr8.3:22906237-22906256	None:intergenic	40.0%
	CACAAAGGAAGATCAATCGA+TGG	+	chr8.3:22905567-22905586	MS.gene60408:CDS	40.0%
	CAGCAATGATTGTAATGTCG+TGG	-	chr8.3:22905804-22905823	None:intergenic	40.0%
	CCAAACATGTCATTAGTGAC+GGG	-	chr8.3:22906991-22907010	None:intergenic	40.0%
	CCGTATAAATCTGTTGGGTT+TGG	-	chr8.3:22904842-22904861	None:intergenic	40.0%
	CGACATTACAATCATTGCTG+AGG	+	chr8.3:22905804-22905823	MS.gene60408:CDS	40.0%
	CTTCTTACCTTCTTTAGGTC+AGG	-	chr8.3:22905088-22905107	None:intergenic	40.0%
	CTTTAGATCGAAGACATACC+AGG	+	chr8.3:22905342-22905361	MS.gene60408:intron	40.0%
	GGGGCAACACAATATTTGAA+AGG	-	chr8.3:22906267-22906286	None:intergenic	40.0%
	TCAGTTTCACAATAGTGGTC+TGG	-	chr8.3:22905607-22905626	None:intergenic	40.0%
	TCTTTGTTCCTCAAAGGCAA+TGG	-	chr8.3:22904612-22904631	None:intergenic	40.0%
	TGAGATGACTGAAAAGAGGA+TGG	-	chr8.3:22904800-22904819	None:intergenic	40.0%
!	ACAATAGTGGTCTGGTTTGT+TGG	-	chr8.3:22905599-22905618	None:intergenic	40.0%
!	ATGTAGCTTTTGCTTTGCCA+TGG	+	chr8.3:22907078-22907097	MS.gene60408:intron	40.0%
!	CAAGAAGTCCATCATGCTTT+TGG	-	chr8.3:22905705-22905724	None:intergenic	40.0%
!	TGTAGCTTTTGCTTTGCCAT+GGG	+	chr8.3:22907079-22907098	MS.gene60408:intron	40.0%
!	TTCAGTGTCATTGCCCAATT+TGG	+	chr8.3:22904889-22904908	MS.gene60408:CDS	40.0%
!!	ATTTTGGGTGTGGTAGTATG+TGG	-	chr8.3:22906438-22906457	None:intergenic	40.0%
!!	TGCAGTTTGTATTTTGGGTG+TGG	-	chr8.3:22906448-22906467	None:intergenic	40.0%
	AAGCTATAACCATGCCAGCT+CGG	+	chr8.3:22906323-22906342	MS.gene60408:intron	45.0%
	AGAGTTTCTCTCACGTGACA+TGG	+	chr8.3:22904580-22904599	MS.gene60408:CDS	45.0%
	ATATATTCGACCGAGACACC+TGG	-	chr8.3:22905363-22905382	None:intergenic	45.0%
	ATTGGGCAATGACACTGAAG+TGG	-	chr8.3:22904888-22904907	None:intergenic	45.0%
	CCCGTCACTAATGACATGTT+TGG	+	chr8.3:22906988-22907007	MS.gene60408:intron	45.0%
	CTGAAAAGAGGATGGAGATG+CGG	-	chr8.3:22904792-22904811	None:intergenic	45.0%
	GAATGTTTCGCTTAGAGTGG+TGG	-	chr8.3:22904657-22904676	None:intergenic	45.0%
	GAGTTCTGCAAGAGCAATGA+AGG	-	chr8.3:22905062-22905081	None:intergenic	45.0%
	GTCGTGGAAAGAATCTCCAA+AGG	-	chr8.3:22905788-22905807	None:intergenic	45.0%
	TCTGCTATAGTGACTGTGAC+GGG	-	chr8.3:22906236-22906255	None:intergenic	45.0%
	TGACATGTTTGGATCAGCGT+TGG	+	chr8.3:22906999-22907018	MS.gene60408:intron	45.0%
	TTGGGCAATGACACTGAAGT+GGG	-	chr8.3:22904887-22904906	None:intergenic	45.0%
!	AAAAGTGCGGTGAATTCCCA+TGG	-	chr8.3:22907098-22907117	None:intergenic	45.0%
!	ATCCACTGCCAAAAGCATGA+TGG	+	chr8.3:22905694-22905713	MS.gene60408:CDS	45.0%
!	GAGAGAAGTATGAACATGGG+CGG	+	chr8.3:22906117-22906136	MS.gene60408:intron	45.0%
!	TGGTGATGCTACTGCTATCA+GGG	+	chr8.3:22905633-22905652	MS.gene60408:CDS	45.0%
!!	AGGGTAGAAGCAAAAGTGTC+AGG	+	chr8.3:22905652-22905671	MS.gene60408:CDS	45.0%
!!	GGGTAGAAGCAAAAGTGTCA+GGG	+	chr8.3:22905653-22905672	MS.gene60408:CDS	45.0%
!!	GGGTTTGGGTTGTCAAAAGA+CGG	-	chr8.3:22904827-22904846	None:intergenic	45.0%
!!	TTGGTGATGCTACTGCTATC+AGG	+	chr8.3:22905632-22905651	MS.gene60408:CDS	45.0%
!!!	AAACGTTTTGGTTACCGAGC+TGG	-	chr8.3:22906340-22906359	None:intergenic	45.0%
	ACTCGTCCAGAATCTACGCA+TGG	+	chr8.3:22907276-22907295	MS.gene60408:CDS	50.0%
	ATGCTGCCATGCGTAGATTC+TGG	-	chr8.3:22907285-22907304	None:intergenic	50.0%
	CGAAGACATACCAGGTGTCT+CGG	+	chr8.3:22905350-22905369	MS.gene60408:intron	50.0%
	TATGAACATGGGCGGATCCA+CGG	+	chr8.3:22906125-22906144	MS.gene60408:intron	50.0%
!	GAACTTGCTTGATGCTGACG+TGG	+	chr8.3:22904553-22904572	MS.gene60408:CDS	50.0%
!	GTCCATCATGCTTTTGGCAG+TGG	-	chr8.3:22905699-22905718	None:intergenic	50.0%
!!	TTTTGGTTACCGAGCTGGCA+TGG	-	chr8.3:22906335-22906354	None:intergenic	50.0%
	ATGAACATGGGCGGATCCAC+GGG	+	chr8.3:22906126-22906145	MS.gene60408:intron	55.0%
	CGGATCCACGGGTTCAATTG+GGG	+	chr8.3:22906137-22906156	MS.gene60408:intron	55.0%
	GCGGATCCACGGGTTCAATT+GGG	+	chr8.3:22906136-22906155	MS.gene60408:intron	55.0%
!	GGCTAGTGCCATTGCCTTTG+AGG	+	chr8.3:22904601-22904620	MS.gene60408:CDS	55.0%
	GCTGTCCCCAATTGAACCCG+TGG	-	chr8.3:22906145-22906164	None:intergenic	60.0%
	GGCGGATCCACGGGTTCAAT+TGG	+	chr8.3:22906135-22906154	MS.gene60408:intron	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	22904527	22907324	22904527	ID=MS.gene60408
chr8.3	mRNA	22904527	22907324	22904527	ID=MS.gene60408.t1;Parent=MS.gene60408
chr8.3	exon	22904527	22905099	22904527	ID=MS.gene60408.t1.exon1;Parent=MS.gene60408.t1
chr8.3	CDS	22904527	22905099	22904527	ID=cds.MS.gene60408.t1;Parent=MS.gene60408.t1
chr8.3	exon	22905475	22905825	22905475	ID=MS.gene60408.t1.exon2;Parent=MS.gene60408.t1
chr8.3	CDS	22905475	22905825	22905475	ID=cds.MS.gene60408.t1;Parent=MS.gene60408.t1
chr8.3	exon	22907244	22907324	22907244	ID=MS.gene60408.t1.exon3;Parent=MS.gene60408.t1
chr8.3	CDS	22907244	22907324	22907244	ID=cds.MS.gene60408.t1;Parent=MS.gene60408.t1

Gene Sequence

>MS.gene60408

ATGTTATTTGACAAGGAATGCTACTTGAACTTGCTTGATGCTGACGTGGTAGAAGAGTTTCTCTCACGTGACATGGCTAGTGCCATTGCCTTTGAGGAACAAAGAGAGACTCTACAACAATGTATTTCCACCACTCTAAGCGAAACATTCAATGGTGAAACAAGTTTTGAGTCTTTTGACTTTGATTTTGAGAATCCAACCAAGAAGCTGAAATTTTTTGATCAAAGTAACAACATAACCAAAAGCTTTTCACAACAAATTTCCGCATCTCCATCCTCTTTTCAGTCATCTCAGATTCCGTCTTTTGACAACCCAAACCCAACAGATTTATACGGGTTTGATCAAAAACAGAAAGAACCCACTTCAGTGTCATTGCCCAATTTGGATAACACACGTTTTTCAGCACAACCCTCAATTGAGTCTTCAAAAAATCAGAACTTGGAAACTAAAACATCACAATCTAAGAGGTCTTCGGCTCATGTAAAAGATCACATTATGGTTGAGAGAAAACGAAGAGAGAAACTCGGTCAAGCCTTCATTGCTCTTGCAGAACTCATTCCTGACCTAAAGAAGGTAAGAAGTAACTGTTAGATAAATGTAGATCTAGTTTCAAAAGAAGTGACATTGTTGATTGTTGCAGACATGCCAATACACATCTTTAACCTATATCTTTAACCATGTATTAGCAAAACTTATAAATATTTGATATTTTGAATATATCCGTCGAAACAAATCTAACATTATACTACCATTTGTTTTTATATATAATAGTAAAAGAATATGGTCAAAGTATCTTATTCTTATAACGACTCACACTTTAGATCGAAGACATACCAGGTGTCTCGGTCGAATATATGTTAATGCAGTTAAATTTGACCACTTTAATTTTCTTAAATTATATAACCTATGACTATGTCTTACTAGTCACGTGTAAAATATGTTAATTAGAAGGACAAGGCTTCAGTATTAGCAGATACAATCAAATACCTCAAAGAGCTTAAAGAGCGTTTGTCAATTTTAGAAGAAGAAAAAGGGAAGAACACAAAGGAAGATCAATCGATGGTGGTTTCCAACAAACCAGACCACTATTGTGAAACTGAAAGTGTTGGTGATGCTACTGCTATCAGGGTAGAAGCAAAAGTGTCAGGGAAAAAGATGTTGATTAGGATCCACTGCCAAAAGCATGATGGACTTCTTGTTAAAGTCATCACTGAGATTCAAAGTTTTCAACTATTAGTTGTCAATAACCGTATTTTAGCCTTTGGAGATTCTTTCCACGACATTACAATCATTGCTGAGGTAAAGGACATTCTCTTTTAATAAAAGACTCAGATTTTTTTTCTTTCTTTCCATTTATTCTCTACATCATCTTTTAAAAAAAAAATATTAATACTCTTTTTATATATCATTTTTTACTAATAAAATATCCAAATCATAGAGTTTATTTTGTTGTGTATTTTTGACTTTGTTTATTTTGTTGTGTATTTTTCACTTTTGTTGTTGAGTAGTGATATTTATAGAACTATTTGATAACATCGACAACTGTTGTGACAATCTTCTTTTTTATCTTTTTATTGGTAAAAAATAATAGAGAGAAGTATGAACATGGGCGGATCCACGGGTTCAATTGGGGACAGCTGACCCATAAGCTTGATTACTTTTTAACCAATCATTAAATATTTTGATGTTTTGCTCTAGAGGACTCTCCCGTCACAGTCACTATAGCAGATTTGCTCCCCTTTCAAATATTGTGTTGCCCCCACAATCTCAACTTATGAAAACATACGATTGAAAAAAAGCTATAACCATGCCAGCTCGGTAACCAAAACGTTTTCATTATTTAAGAGAAGCCATGCCATTATTCTCTTTGTCAAAAAAGGGATGCTAATAAATAAATAAAATACTTCTCCACATACTACCACACCCAAAATACAAACTGCAAGCTTTTTACTCGTATTTTTTGTATCATGAACTTAAATTGTAGGGCTTAATACAAGTAAAAAGGGTTTTCATAGTAATAAATTTTGTGAAATATGAACTTTTCATAGTTTATATGGAGATATTTTTGAATGATTGTCTAGAGTAGTAATATTTGATAGTGTTGAAAGATAGTTATCTAATTTTTTTTTTTTTTTATATGTAATGCTATAATTTTTTTATCTCCATTGTTTTTTTAAAGAGAATGATACCATCATTTTATCGCTTAAACATTTATATAATTTTTGTATGAAAACCACTGTTCTCTCATTTATATTTGAGAATCTATTTCTCAAATATTCACTCATTTTATAAAAAATTAGGTCCATATTTTAGGAATAGATTCTACGTAAAGTCGTGAAACCTATATGAATTTCATCTAATAAAAGAGTGAATATTTGAGAAAATGTTCATAACTTTTCTCTTTTTGTGTGGATTTCTTAATAAATAAATCACTATGGCCCCATATATAAAAATTTCTAGACCCGTCACTAATGACATGTTTGGATCAGCGTTGGCATGCCAAATTTCACTTCCCAATGCATGTTAGACGTGATATTTATGAAGCTACAATATGTAGCTTTTGCTTTGCCATGGGAATTCACCGCACTTTTGTAAAAACAAACGTGTATCTAAACACTATCTAAGTATGAATGAGAAAGTTGTTTAATGGTTGTATAATTTCTCTCTGGCGTCACCACTTATTTGTATTGATTGTTAATTCTATTATTGTTTTACAGATTGGTGAAGGCTACAACTTGACCTTAAAAGAACTCGTCCAGAATCTACGCATGGCAGCATTGAAGTTTATGTCATCGTAA

Protein sequence

>MS.gene60408.t1

MLFDKECYLNLLDADVVEEFLSRDMASAIAFEEQRETLQQCISTTLSETFNGETSFESFDFDFENPTKKLKFFDQSNNITKSFSQQISASPSSFQSSQIPSFDNPNPTDLYGFDQKQKEPTSVSLPNLDNTRFSAQPSIESSKNQNLETKTSQSKRSSAHVKDHIMVERKRREKLGQAFIALAELIPDLKKKDKASVLADTIKYLKELKERLSILEEEKGKNTKEDQSMVVSNKPDHYCETESVGDATAIRVEAKVSGKKMLIRIHCQKHDGLLVKVITEIQSFQLLVVNNRILAFGDSFHDITIIAEIGEGYNLTLKELVQNLRMAALKFMSS