AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene60526

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60526.t1 MTR_1g090913 97.394 307 7 1 1 307 1 306 0.0 602
MS.gene60526.t1 MTR_3g465120 46.396 222 118 1 84 305 81 301 8.54e-71 221
MS.gene60526.t1 MTR_4g015990 42.917 240 132 2 70 305 1 239 3.75e-68 213
MS.gene60526.t1 MTR_4g016040 44.976 209 114 1 92 300 27 234 1.54e-62 198
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene60526.t1 AT5G04490 66.667 240 80 0 68 307 65 304 8.44e-117 338
MS.gene60526.t1 AT5G04490 70.787 178 52 0 68 245 65 242 5.45e-91 271
MS.gene60526.t1 AT5G58560 42.793 222 126 1 84 305 87 307 5.13e-66 209

Find 75 sgRNAs with CRISPR-Local

Find 132 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCATAGCAATGCTTATATT+TGG 0.177370 1.2:+68501023 MS.gene60526:CDS
AATGCTTATATTTGGATTCT+TGG 0.197393 1.2:+68501031 MS.gene60526:CDS
TTTGGATTCTTGGTTTCAAT+TGG 0.256377 1.2:+68501041 MS.gene60526:CDS
GGTGCCACTGTTGGTGTTCT+TGG 0.284632 1.2:+68500006 MS.gene60526:CDS
GATATCATTGGTAGAAGATA+TGG 0.303985 1.2:+68500957 MS.gene60526:CDS
AAAGCAATCCTGATAATATA+TGG 0.321784 1.2:-68500325 None:intergenic
GAAATGGGGTACCTGAAAAT+TGG 0.330597 1.2:-68500362 None:intergenic
TTCTGTTCCACTAGTAACTA+TGG 0.342085 1.2:+68501359 MS.gene60526:CDS
TTACAAATCTTTCATGTAAA+CGG 0.355839 1.2:-68499936 None:intergenic
TCCAATTGGGGTTGTTGCCT+TGG 0.359168 1.2:+68500798 MS.gene60526:CDS
TGTTAAGTGCTATTGTGTTC+TGG 0.376561 1.2:+68500767 MS.gene60526:CDS
AAACTGGTCCATATATTATC+AGG 0.380119 1.2:+68500317 MS.gene60526:CDS
TTCTGGCGTGAATCTCCAAT+TGG 0.390436 1.2:+68500784 MS.gene60526:CDS
TTGGATTCTTGGTTTCAATT+GGG 0.404183 1.2:+68501042 MS.gene60526:CDS
ATTAGATCTTGAGCATTTGC+AGG 0.415465 1.2:-68499973 None:intergenic
CTCTCCAAAGTTATTAATGA+TGG 0.428032 1.2:-68501401 None:intergenic
ACAGTGTCGTTTGTTGTTGT+GGG 0.430951 1.2:-68499877 None:intergenic
TTTGGTGAATGGTCTCTCCT+TGG 0.432501 1.2:+68500562 MS.gene60526:CDS
TCTGGCGTGAATCTCCAATT+GGG 0.433764 1.2:+68500785 MS.gene60526:CDS
CGGCGGTGCACGGTGGAGAA+TGG 0.436792 1.2:-68499811 None:intergenic
GACAGTGTCGTTTGTTGTTG+TGG 0.438491 1.2:-68499878 None:intergenic
TCATCGACCACCTCAGTAAT+GGG 0.442023 1.2:-68501330 None:intergenic
GCAGGGTCTGAGCAGAAAAC+TGG 0.450996 1.2:+68500301 MS.gene60526:intron
CTCTCCTTGGTTACTGATCA+AGG 0.451114 1.2:+68500575 MS.gene60526:CDS
GCCAAGGCAACAACCCCAAT+TGG 0.456276 1.2:-68500799 None:intergenic
CACCGTGCACCGCCGCTCAC+CGG 0.464001 1.2:+68499819 MS.gene60526:CDS
GTGGTGGTGGTAGTGGTTGC+CGG 0.465060 1.2:-68499838 None:intergenic
CAGCAAAGTAACGAGCTTCA+AGG 0.476437 1.2:-68500508 None:intergenic
TTAATATCCCAACATAATAG+AGG 0.477271 1.2:-68500744 None:intergenic
TGCCGGTGAGCGGCGGTGCA+CGG 0.481610 1.2:-68499821 None:intergenic
GAGGTCCCCTCTATTATGTT+GGG 0.482902 1.2:+68500737 MS.gene60526:CDS
GACATGTGCAGTTTGATTTG+TGG 0.485754 1.2:+68501250 MS.gene60526:CDS
GTTGTTGTGGGTTTGTTCCA+AGG 0.487703 1.2:-68499865 None:intergenic
CTGTACTACTATTCAGCTCT+AGG 0.488201 1.2:+68501228 MS.gene60526:CDS
GTCATCGACCACCTCAGTAA+TGG 0.501122 1.2:-68501331 None:intergenic
GCCACTGTTGGTGTTCTTGG+CGG 0.506473 1.2:+68500009 MS.gene60526:CDS
CTAGGTGTAGCTGATATCAT+TGG 0.520300 1.2:+68500945 MS.gene60526:intron
AGCAAAGTAACGAGCTTCAA+GGG 0.523609 1.2:-68500507 None:intergenic
AGAGGTCCCCTCTATTATGT+TGG 0.534169 1.2:+68500736 MS.gene60526:CDS
AGGAAAAGTGGTGGTGGTAG+TGG 0.535981 1.2:-68499845 None:intergenic
AAAGACACAAAAGCAACTCT+TGG 0.543465 1.2:-68501282 None:intergenic
ATATCATTGGTAGAAGATAT+GGG 0.547601 1.2:+68500958 MS.gene60526:CDS
ATCAAATCTGTGACTAGGAA+AGG 0.549956 1.2:+68500602 MS.gene60526:CDS
CACAATGTTGGTGCCACTGT+TGG 0.565061 1.2:+68499997 MS.gene60526:CDS
GCCTTGGCAATGATGTGTGG+AGG 0.567586 1.2:+68500814 MS.gene60526:CDS
CCTTGGCAATGATGTGTGGA+GGG 0.571638 1.2:+68500815 MS.gene60526:CDS
TAATATCCCAACATAATAGA+GGG 0.574859 1.2:-68500743 None:intergenic
GCAATGATGTGTGGAGGGGA+TGG 0.575045 1.2:+68500820 MS.gene60526:CDS
TTAGATCTTGAGCATTTGCA+GGG 0.579429 1.2:-68499972 None:intergenic
TGGTAGTGGTTGCCGGTGAG+CGG 0.581305 1.2:-68499831 None:intergenic
CGACCACCTCAGTAATGGGT+AGG 0.581372 1.2:-68501326 None:intergenic
CCCTACAGAGAATTGCTAAG+AGG 0.586996 1.2:+68500718 MS.gene60526:intron
GTCACTGCCATAGTTACTAG+TGG 0.589305 1.2:-68501366 None:intergenic
GTTGCCTTGGCAATGATGTG+TGG 0.591787 1.2:+68500811 MS.gene60526:CDS
TACATGAAAGATTTGTAACA+AGG 0.593372 1.2:+68499941 MS.gene60526:CDS
GGGTTTGTTCCAAGGAAAAG+TGG 0.594094 1.2:-68499857 None:intergenic
CCCTCCACACATCATTGCCA+AGG 0.594888 1.2:-68500815 None:intergenic
CAAGATCTAATCCACAATGT+TGG 0.596614 1.2:+68499985 MS.gene60526:CDS
GTTCCAAGGAAAAGTGGTGG+TGG 0.600381 1.2:-68499851 None:intergenic
CAACAGTGGCACCAACATTG+TGG 0.602793 1.2:-68499996 None:intergenic
GGAGAATGGAAAGAGGTGAG+AGG 0.607112 1.2:-68499797 None:intergenic
GTCCCTACCCATTACTGAGG+TGG 0.610171 1.2:+68501323 MS.gene60526:CDS
GACTCATCAAATCTGTGACT+AGG 0.615495 1.2:+68500597 MS.gene60526:CDS
GACCACCTCAGTAATGGGTA+GGG 0.617402 1.2:-68501325 None:intergenic
GCACGGTGGAGAATGGAAAG+AGG 0.620238 1.2:-68499804 None:intergenic
GCCGCCAAGAACACCAACAG+TGG 0.621865 1.2:-68500010 None:intergenic
CTTGGCAATGATGTGTGGAG+GGG 0.628426 1.2:+68500816 MS.gene60526:CDS
CGGACACTACGATGTACTGA+AGG 0.643227 1.2:-68499916 None:intergenic
TAGTGGTTGCCGGTGAGCGG+CGG 0.650216 1.2:-68499828 None:intergenic
CTGGCGTGAATCTCCAATTG+GGG 0.652571 1.2:+68500786 MS.gene60526:CDS
CGGTGAGCGGCGGTGCACGG+TGG 0.655658 1.2:-68499818 None:intergenic
GGAGTCCCTACCCATTACTG+AGG 0.664036 1.2:+68501320 MS.gene60526:CDS
TTTGTTCCAAGGAAAAGTGG+TGG 0.669852 1.2:-68499854 None:intergenic
GAGTCCTTGATCAGTAACCA+AGG 0.675558 1.2:-68500579 None:intergenic
AATATCCCAACATAATAGAG+GGG 0.697645 1.2:-68500742 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATGTCTTTATTCATTTTTT+TGG + chr1.2:68500644-68500663 MS.gene60526:intron 15.0%
!! ATTTATCTAAATCACAGAAA+TGG - chr1.2:68500381-68500400 None:intergenic 20.0%
!! TTAATCAACATTGAATTTGA+TGG + chr1.2:68500919-68500938 MS.gene60526:intron 20.0%
!! TTACAAATCTTTCATGTAAA+CGG - chr1.2:68499939-68499958 None:intergenic 20.0%
!! TTCCTTATAAGGAAAATTTA+AGG + chr1.2:68500433-68500452 MS.gene60526:intron 20.0%
!! TTTATCTAAATCACAGAAAT+GGG - chr1.2:68500380-68500399 None:intergenic 20.0%
!!! GTCAATTCATATATTTTTCT+TGG - chr1.2:68500170-68500189 None:intergenic 20.0%
!!! TTCATTTTTTTGGCATTATT+TGG + chr1.2:68500654-68500673 MS.gene60526:intron 20.0%
! AAAAAAATTGCAAAGCTTCT+AGG - chr1.2:68500901-68500920 None:intergenic 25.0%
! AAAAAATTGCAAAGCTTCTA+GGG - chr1.2:68500900-68500919 None:intergenic 25.0%
! AAACATCATTGATCAAGTAA+TGG + chr1.2:68500074-68500093 MS.gene60526:intron 25.0%
! AAAGCAATCCTGATAATATA+TGG - chr1.2:68500328-68500347 None:intergenic 25.0%
! AAGACATTTGATACTTACTT+TGG - chr1.2:68500632-68500651 None:intergenic 25.0%
! AATGCTTATATTTGGATTCT+TGG + chr1.2:68501031-68501050 MS.gene60526:CDS 25.0%
! ATAAAGCAATGTGTAAAGAA+AGG - chr1.2:68500853-68500872 None:intergenic 25.0%
! ATATCATTGGTAGAAGATAT+GGG + chr1.2:68500958-68500977 MS.gene60526:CDS 25.0%
! GAACCAAAACAAGTAAAAAA+TGG - chr1.2:68500199-68500218 None:intergenic 25.0%
! TAATATCCCAACATAATAGA+GGG - chr1.2:68500746-68500765 None:intergenic 25.0%
! TACATGAAAGATTTGTAACA+AGG + chr1.2:68499941-68499960 MS.gene60526:CDS 25.0%
! TATTTCTTAAACCTTTCCTA+GGG + chr1.2:68500870-68500889 MS.gene60526:intron 25.0%
! TTAATATCCCAACATAATAG+AGG - chr1.2:68500747-68500766 None:intergenic 25.0%
! TTACAACCAAAAAAAGAGTT+GGG + chr1.2:68500995-68501014 MS.gene60526:CDS 25.0%
! TTATCTAAATCACAGAAATG+GGG - chr1.2:68500379-68500398 None:intergenic 25.0%
!! ATAAGGAAAATTTAAGGCAT+TGG + chr1.2:68500439-68500458 MS.gene60526:intron 25.0%
!! TCTTTGCTTTATTCCTTATA+AGG + chr1.2:68500422-68500441 MS.gene60526:intron 25.0%
!! TGCCTTAAATTTTCCTTATA+AGG - chr1.2:68500438-68500457 None:intergenic 25.0%
!! TTATTTCTTAAACCTTTCCT+AGG + chr1.2:68500869-68500888 MS.gene60526:intron 25.0%
!!! AAAAAGTACTGTTGAAAAAG+TGG - chr1.2:68501089-68501108 None:intergenic 25.0%
!!! AATGTTTTGGTGTTAATGTT+AGG + chr1.2:68500475-68500494 MS.gene60526:intron 25.0%
!!! ATTGCTTTGTTTGAATGTTT+TGG + chr1.2:68500462-68500481 MS.gene60526:intron 25.0%
!!! GATCCATTTTTTACTTGTTT+TGG + chr1.2:68500193-68500212 MS.gene60526:intron 25.0%
AAACTGGTCCATATATTATC+AGG + chr1.2:68500317-68500336 MS.gene60526:CDS 30.0%
AATATCCCAACATAATAGAG+GGG - chr1.2:68500745-68500764 None:intergenic 30.0%
AGCATAGCAATGCTTATATT+TGG + chr1.2:68501023-68501042 MS.gene60526:CDS 30.0%
AGTGTGAAAATCTATCTACT+TGG - chr1.2:68501177-68501196 None:intergenic 30.0%
CTTACAACCAAAAAAAGAGT+TGG + chr1.2:68500994-68501013 MS.gene60526:CDS 30.0%
GATATCATTGGTAGAAGATA+TGG + chr1.2:68500957-68500976 MS.gene60526:CDS 30.0%
TCATTGATCAAGTAATGGTT+TGG + chr1.2:68500079-68500098 MS.gene60526:intron 30.0%
TTGGATTCTTGGTTTCAATT+GGG + chr1.2:68501042-68501061 MS.gene60526:CDS 30.0%
TTTGGATTCTTGGTTTCAAT+TGG + chr1.2:68501041-68501060 MS.gene60526:CDS 30.0%
! CATTGAATTTGATGGTTACT+AGG + chr1.2:68500927-68500946 MS.gene60526:intron 30.0%
!! ATCTGTGTTTGCTTTGAAAT+TGG + chr1.2:68500263-68500282 MS.gene60526:intron 30.0%
!! TTTTTGGCATTATTTGGTCA+TGG + chr1.2:68500660-68500679 MS.gene60526:intron 30.0%
!!! ATTATCAGGATTGCTTTTTC+TGG + chr1.2:68500331-68500350 MS.gene60526:CDS 30.0%
!!! CAACTCTTTTTTTGGTTGTA+AGG - chr1.2:68500996-68501015 None:intergenic 30.0%
AAAGACACAAAAGCAACTCT+TGG - chr1.2:68501285-68501304 None:intergenic 35.0%
ATCAAATCTGTGACTAGGAA+AGG + chr1.2:68500602-68500621 MS.gene60526:CDS 35.0%
ATTAGATCTTGAGCATTTGC+AGG - chr1.2:68499976-68499995 None:intergenic 35.0%
CAAGATCTAATCCACAATGT+TGG + chr1.2:68499985-68500004 MS.gene60526:CDS 35.0%
TAGGGTATGTAAACAGTACA+AGG - chr1.2:68500703-68500722 None:intergenic 35.0%
TGCATGCAAATTGCAAAGTA+AGG - chr1.2:68500131-68500150 None:intergenic 35.0%
TGCTGATATTTATTGCATGC+AGG + chr1.2:68501202-68501221 MS.gene60526:intron 35.0%
TTAGATCTTGAGCATTTGCA+GGG - chr1.2:68499975-68499994 None:intergenic 35.0%
TTCTGTTCCACTAGTAACTA+TGG + chr1.2:68501359-68501378 MS.gene60526:CDS 35.0%
! TGTTAAGTGCTATTGTGTTC+TGG + chr1.2:68500767-68500786 MS.gene60526:CDS 35.0%
!!! AGTTGCTTTTGTGTCTTTTG+TGG + chr1.2:68501287-68501306 MS.gene60526:CDS 35.0%
!!! GATTGCTTTTTCTGGTTTCA+TGG + chr1.2:68500339-68500358 MS.gene60526:CDS 35.0%
!!! GGTGAATTTTTTGAGGCTTT+TGG + chr1.2:68500544-68500563 MS.gene60526:CDS 35.0%
!!! TTCCTCTGGTGAATTTTTTG+AGG + chr1.2:68500537-68500556 MS.gene60526:CDS 35.0%
!!! TTTTTGAGGCTTTTGGTGAA+TGG + chr1.2:68500551-68500570 MS.gene60526:CDS 35.0%
AACCAAAAAAAGAGTTGGGC+TGG + chr1.2:68500999-68501018 MS.gene60526:CDS 40.0%
ACCTCTTAGCAATTCTCTGT+AGG - chr1.2:68500722-68500741 None:intergenic 40.0%
AGCAAAGTAACGAGCTTCAA+GGG - chr1.2:68500510-68500529 None:intergenic 40.0%
AGCCTCAAAAAATTCACCAG+AGG - chr1.2:68500542-68500561 None:intergenic 40.0%
CCTCTTAGCAATTCTCTGTA+GGG - chr1.2:68500721-68500740 None:intergenic 40.0%
CTAGGTGTAGCTGATATCAT+TGG + chr1.2:68500945-68500964 MS.gene60526:intron 40.0%
CTGTACTACTATTCAGCTCT+AGG + chr1.2:68501228-68501247 MS.gene60526:CDS 40.0%
GACTCATCAAATCTGTGACT+AGG + chr1.2:68500597-68500616 MS.gene60526:CDS 40.0%
TGGCATTATTTGGTCATGGA+AGG + chr1.2:68500664-68500683 MS.gene60526:intron 40.0%
TTTGTTCCAAGGAAAAGTGG+TGG - chr1.2:68499857-68499876 None:intergenic 40.0%
! GAAATGGGGTACCTGAAAAT+TGG - chr1.2:68500365-68500384 None:intergenic 40.0%
! TGGTTTCATGGCCAATTTTC+AGG + chr1.2:68500351-68500370 MS.gene60526:CDS 40.0%
!! AAATTGGTGTCACATGGTGA+TGG + chr1.2:68500279-68500298 MS.gene60526:intron 40.0%
!! ACAGTGTCGTTTGTTGTTGT+GGG - chr1.2:68499880-68499899 None:intergenic 40.0%
!! GACATGTGCAGTTTGATTTG+TGG + chr1.2:68501250-68501269 MS.gene60526:CDS 40.0%
!! GCTTTGAAATTGGTGTCACA+TGG + chr1.2:68500273-68500292 MS.gene60526:intron 40.0%
!!! TACCAGCCCAACTCTTTTTT+TGG - chr1.2:68501004-68501023 None:intergenic 40.0%
AGAGGTCCCCTCTATTATGT+TGG + chr1.2:68500736-68500755 MS.gene60526:CDS 45.0%
CAGCAAAGTAACGAGCTTCA+AGG - chr1.2:68500511-68500530 None:intergenic 45.0%
CCCTACAGAGAATTGCTAAG+AGG + chr1.2:68500718-68500737 MS.gene60526:intron 45.0%
CTCTCCTTGGTTACTGATCA+AGG + chr1.2:68500575-68500594 MS.gene60526:CDS 45.0%
GAGGTCCCCTCTATTATGTT+GGG + chr1.2:68500737-68500756 MS.gene60526:CDS 45.0%
GAGTCCTTGATCAGTAACCA+AGG - chr1.2:68500582-68500601 None:intergenic 45.0%
GTCACTGCCATAGTTACTAG+TGG - chr1.2:68501369-68501388 None:intergenic 45.0%
TCATCGACCACCTCAGTAAT+GGG - chr1.2:68501333-68501352 None:intergenic 45.0%
TCTAGGGAAATCCCTAGGAA+AGG - chr1.2:68500884-68500903 None:intergenic 45.0%
TCTGGCGTGAATCTCCAATT+GGG + chr1.2:68500785-68500804 MS.gene60526:CDS 45.0%
TTCTGGCGTGAATCTCCAAT+TGG + chr1.2:68500784-68500803 MS.gene60526:CDS 45.0%
TTTGGTGAATGGTCTCTCCT+TGG + chr1.2:68500562-68500581 MS.gene60526:CDS 45.0%
! AAGCTTCTAGGGAAATCCCT+AGG - chr1.2:68500889-68500908 None:intergenic 45.0%
! GGGTTTGTTCCAAGGAAAAG+TGG - chr1.2:68499860-68499879 None:intergenic 45.0%
! GTTGTTGTGGGTTTGTTCCA+AGG - chr1.2:68499868-68499887 None:intergenic 45.0%
! TGTGTCTTTTGTGGCAACAG+TGG + chr1.2:68501296-68501315 MS.gene60526:CDS 45.0%
!! GACAGTGTCGTTTGTTGTTG+TGG - chr1.2:68499881-68499900 None:intergenic 45.0%
!!! CTTTGCTGCTTTTGTTCCTC+TGG + chr1.2:68500523-68500542 MS.gene60526:CDS 45.0%
!!! TTGTTTATTTTTTTTTTTAA+TGG + chr1.2:68500236-68500255 MS.gene60526:intron 5.0%
CAACAGTGGCACCAACATTG+TGG - chr1.2:68499999-68500018 None:intergenic 50.0%
CCTTGGCAATGATGTGTGGA+GGG + chr1.2:68500815-68500834 MS.gene60526:CDS 50.0%
CGGACACTACGATGTACTGA+AGG - chr1.2:68499919-68499938 None:intergenic 50.0%
CTGGCGTGAATCTCCAATTG+GGG + chr1.2:68500786-68500805 MS.gene60526:CDS 50.0%
CTTGGCAATGATGTGTGGAG+GGG + chr1.2:68500816-68500835 MS.gene60526:CDS 50.0%
GGAGAATGGAAAGAGGTGAG+AGG - chr1.2:68499800-68499819 None:intergenic 50.0%
GTCATCGACCACCTCAGTAA+TGG - chr1.2:68501334-68501353 None:intergenic 50.0%
GTTCCAAGGAAAAGTGGTGG+TGG - chr1.2:68499854-68499873 None:intergenic 50.0%
GTTGCCTTGGCAATGATGTG+TGG + chr1.2:68500811-68500830 MS.gene60526:CDS 50.0%
TCCAATTGGGGTTGTTGCCT+TGG + chr1.2:68500798-68500817 MS.gene60526:CDS 50.0%
! GTCTTTTGTGGCAACAGTGG+TGG + chr1.2:68501299-68501318 MS.gene60526:CDS 50.0%
!! AGGAAAAGTGGTGGTGGTAG+TGG - chr1.2:68499848-68499867 None:intergenic 50.0%
!! CACAATGTTGGTGCCACTGT+TGG + chr1.2:68499997-68500016 MS.gene60526:CDS 50.0%
!! CTACCACCACCACTTTTCCT+TGG + chr1.2:68499848-68499867 MS.gene60526:CDS 50.0%
!! GACCACCTCAGTAATGGGTA+GGG - chr1.2:68501328-68501347 None:intergenic 50.0%
CCCTCCACACATCATTGCCA+AGG - chr1.2:68500818-68500837 None:intergenic 55.0%
GCAATGATGTGTGGAGGGGA+TGG + chr1.2:68500820-68500839 MS.gene60526:CDS 55.0%
GCACGGTGGAGAATGGAAAG+AGG - chr1.2:68499807-68499826 None:intergenic 55.0%
GCAGGGTCTGAGCAGAAAAC+TGG + chr1.2:68500301-68500320 MS.gene60526:intron 55.0%
GCCAAGGCAACAACCCCAAT+TGG - chr1.2:68500802-68500821 None:intergenic 55.0%
GCCTTGGCAATGATGTGTGG+AGG + chr1.2:68500814-68500833 MS.gene60526:CDS 55.0%
GGAGTCCCTACCCATTACTG+AGG + chr1.2:68501320-68501339 MS.gene60526:CDS 55.0%
GTCCCTACCCATTACTGAGG+TGG + chr1.2:68501323-68501342 MS.gene60526:CDS 55.0%
! GCCACTGTTGGTGTTCTTGG+CGG + chr1.2:68500009-68500028 MS.gene60526:CDS 55.0%
!! CGACCACCTCAGTAATGGGT+AGG - chr1.2:68501329-68501348 None:intergenic 55.0%
!! GGTGCCACTGTTGGTGTTCT+TGG + chr1.2:68500006-68500025 MS.gene60526:CDS 55.0%
!! GGTGTCACATGGTGATGGCA+GGG + chr1.2:68500284-68500303 MS.gene60526:intron 55.0%
!! TGGTGTCACATGGTGATGGC+AGG + chr1.2:68500283-68500302 MS.gene60526:intron 55.0%
GCCGCCAAGAACACCAACAG+TGG - chr1.2:68500013-68500032 None:intergenic 60.0%
!! GTGGTGGTGGTAGTGGTTGC+CGG - chr1.2:68499841-68499860 None:intergenic 60.0%
!! TGGTAGTGGTTGCCGGTGAG+CGG - chr1.2:68499834-68499853 None:intergenic 60.0%
TAGTGGTTGCCGGTGAGCGG+CGG - chr1.2:68499831-68499850 None:intergenic 65.0%
CGGCGGTGCACGGTGGAGAA+TGG - chr1.2:68499814-68499833 None:intergenic 70.0%
CACCGTGCACCGCCGCTCAC+CGG + chr1.2:68499819-68499838 MS.gene60526:CDS 75.0%
TGCCGGTGAGCGGCGGTGCA+CGG - chr1.2:68499824-68499843 None:intergenic 75.0%
! CGGTGAGCGGCGGTGCACGG+TGG - chr1.2:68499821-68499840 None:intergenic 80.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 68499784 68501410 68499784 ID=MS.gene60526
chr1.2 mRNA 68499784 68501410 68499784 ID=MS.gene60526.t1;Parent=MS.gene60526
chr1.2 exon 68499784 68500089 68499784 ID=MS.gene60526.t1.exon1;Parent=MS.gene60526.t1
chr1.2 CDS 68499784 68500089 68499784 ID=cds.MS.gene60526.t1;Parent=MS.gene60526.t1
chr1.2 exon 68500305 68500372 68500305 ID=MS.gene60526.t1.exon2;Parent=MS.gene60526.t1
chr1.2 CDS 68500305 68500372 68500305 ID=cds.MS.gene60526.t1;Parent=MS.gene60526.t1
chr1.2 exon 68500502 68500633 68500502 ID=MS.gene60526.t1.exon3;Parent=MS.gene60526.t1
chr1.2 CDS 68500502 68500633 68500502 ID=cds.MS.gene60526.t1;Parent=MS.gene60526.t1
chr1.2 exon 68500726 68500841 68500726 ID=MS.gene60526.t1.exon4;Parent=MS.gene60526.t1
chr1.2 CDS 68500726 68500841 68500726 ID=cds.MS.gene60526.t1;Parent=MS.gene60526.t1
chr1.2 exon 68500949 68501063 68500949 ID=MS.gene60526.t1.exon5;Parent=MS.gene60526.t1
chr1.2 CDS 68500949 68501063 68500949 ID=cds.MS.gene60526.t1;Parent=MS.gene60526.t1
chr1.2 exon 68501224 68501410 68501224 ID=MS.gene60526.t1.exon6;Parent=MS.gene60526.t1
chr1.2 CDS 68501224 68501410 68501224 ID=cds.MS.gene60526.t1;Parent=MS.gene60526.t1
Gene Sequence

>MS.gene60526

ATGACTCTCTTATCCTCTCACCTCTTTCCATTCTCCACCGTGCACCGCCGCTCACCGGCAACCACTACCACCACCACTTTTCCTTGGAACAAACCCACAACAACAAACGACACTGTCAAGTTTTTGTATTCTCCTTCAGTACATCGTAGTGTCCGTTTACATGAAAGATTTGTAACAAGGTTTATTGTCCCTGCAAATGCTCAAGATCTAATCCACAATGTTGGTGCCACTGTTGGTGTTCTTGGCGGCGCTTATGCTCTTGTTTTTTCTTTTGATGATCTAACTAGAAGAAACATCATTGATCAAGTAATGGTTTGGTTTGTATTGTTTTCTTCAATTCATTTCCTTACTTTGCAATTTGCATGCATATATGCACACAATTTCCAAGAAAAATATATGAATTGACATTGATCCATTTTTTACTTGTTTTGGTTCAATTTAAATTTTGAGTTTTGTTTATTTTTTTTTTTAATGGAAAAATCTGTGTTTGCTTTGAAATTGGTGTCACATGGTGATGGCAGGGTCTGAGCAGAAAACTGGTCCATATATTATCAGGATTGCTTTTTCTGGTTTCATGGCCAATTTTCAGGTACCCCATTTCTGTGATTTAGATAAATTTTGATTCTTTTTTATAAATGTCTTTGCTTTATTCCTTATAAGGAAAATTTAAGGCATTGGATTGCTTTGTTTGAATGTTTTGGTGTTAATGTTAGGATAGCAACTCCCTTGAAGCTCGTTACTTTGCTGCTTTTGTTCCTCTGGTGAATTTTTTGAGGCTTTTGGTGAATGGTCTCTCCTTGGTTACTGATCAAGGACTCATCAAATCTGTGACTAGGAAAGGAGATCCAAAGTAAGTATCAAATGTCTTTATTCATTTTTTTGGCATTATTTGGTCATGGAAGGATAAATGTACTAACCTTGTACTGTTTACATACCCTACAGAGAATTGCTAAGAGGTCCCCTCTATTATGTTGGGATATTAATGTTAAGTGCTATTGTGTTCTGGCGTGAATCTCCAATTGGGGTTGTTGCCTTGGCAATGATGTGTGGAGGGGATGGTAATACACCTTTCTTTACACATTGCTTTATTTCTTAAACCTTTCCTAGGGATTTCCCTAGAAGCTTTGCAATTTTTTTAATCAACATTGAATTTGATGGTTACTAGGTGTAGCTGATATCATTGGTAGAAGATATGGGTCAATGAAGATTCCTTACAACCAAAAAAAGAGTTGGGCTGGTAGCATAGCAATGCTTATATTTGGATTCTTGGTTTCAATTGGGTGTGTTACATCACATATTCATCCACTTTTTCAACAGTACTTTTTTTTTTTTTTTACTCATTACGAAATTGACTAAGCTCAGAGAATTAAATGTATTCATATATTTTACTCCAAGTAGATAGATTTTCACACTGAAACTGCTGATATTTATTGCATGCAGGATGCTGTACTACTATTCAGCTCTAGGACATGTGCAGTTTGATTTGTGGAACGTTGTGCCAAGAGTTGCTTTTGTGTCTTTTGTGGCAACAGTGGTGGAGTCCCTACCCATTACTGAGGTGGTCGATGACAACATTTCTGTTCCACTAGTAACTATGGCAGTGACTTTTTTAACTTTCCATCATTAA
Protein sequence

>MS.gene60526.t1

MTLLSSHLFPFSTVHRRSPATTTTTTFPWNKPTTTNDTVKFLYSPSVHRSVRLHERFVTRFIVPANAQDLIHNVGATVGVLGGAYALVFSFDDLTRRNIIDQGLSRKLVHILSGLLFLVSWPIFSNSLEARYFAAFVPLVNFLRLLVNGLSLVTDQGLIKSVTRKGDPKELLRGPLYYVGILMLSAIVFWRESPIGVVALAMMCGGDGVADIIGRRYGSMKIPYNQKKSWAGSIAMLIFGFLVSIGMLYYYSALGHVQFDLWNVVPRVAFVSFVATVVESLPITEVVDDNISVPLVTMAVTFLTFHH