AlfalfaGEDB | MS.gene61422

M. sativa cultivar XinJiangDaYe / MS.gene61422

KEGG: K03627
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF01381.25 PF08523.13

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61422.t1	AES76734.2	97.3	146	4	0	1	146	4	149	2.60E-70	274.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61422.t1	Q9LV58	71.6	148	40	1	1	146	1	148	8.2e-49	194.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61422.t1	G7KNH9	97.3	146	4	0	1	146	4	149	1.9e-70	274.6

TFs/TRs:

Gene ID	Type	Classification
MS.gene61422.t1	TR	MBF1

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene006697	MS.gene61422	PPI
MS.gene94585	MS.gene61422	PPI
MS.gene045483	MS.gene61422	PPI
MS.gene61422	MS.gene73865	PPI
MS.gene61422	MS.gene35285	PPI
MS.gene35285	MS.gene61422	PPI
MS.gene91241	MS.gene61422	PPI
MS.gene73865	MS.gene61422	PPI
MS.gene073154	MS.gene61422	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61422.t1	MTR_6g086280	97.260	146	4	0	1	146	4	149	1.78e-100	285
MS.gene61422.t1	MTR_6g086280	97.222	144	4	0	1	144	4	147	4.12e-99	281
MS.gene61422.t1	MTR_2g084220	50.000	138	69	0	6	143	2	139	4.64e-43	139
MS.gene61422.t1	MTR_4g080090	50.725	138	68	0	6	143	4	141	9.39e-42	136
MS.gene61422.t1	MTR_6g018330	42.105	133	61	1	11	143	26	142	1.70e-26	97.4

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61422.t1	AT3G24500	71.622	148	40	1	1	146	1	148	9.93e-69	204
MS.gene61422.t1	AT3G58680	52.174	138	66	0	6	143	4	141	4.19e-44	142
MS.gene61422.t1	AT2G42680	50.725	138	68	0	6	143	4	141	7.40e-43	139
MS.gene61422.t1	AT3G24500	58.947	95	37	1	1	93	1	95	8.16e-33	112

Find 43 sgRNAs with CRISPR-Local

Find 59 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTTCGGAGGGCTTGGTTTA+TGG	0.142766	6.3:-79722258	None:intergenic
GGGGAAAATGGAGAAGGTTT+TGG	0.237217	6.3:+79722547	MS.gene61422:CDS
TGTTATTAATCAAGGTGTTT+TGG	0.256794	6.3:+79722526	MS.gene61422:CDS
GTTAAACTTAGGGGTAAAAT+TGG	0.277746	6.3:+79722572	MS.gene61422:CDS
CTGCTCCGGTTCGGAGGGCT+TGG	0.299889	6.3:-79722265	None:intergenic
AGGGCTTGGTTTATGGCCTT+TGG	0.306456	6.3:-79722251	None:intergenic
TTACGAAGGTCTTGTGCTTT+GGG	0.313592	6.3:-79722227	None:intergenic
TGAAAGGGTTCAGGTTGTTC+AGG	0.318932	6.3:+79722484	MS.gene61422:CDS
GTTACGAAGGTCTTGTGCTT+TGG	0.324998	6.3:-79722228	None:intergenic
CGCGATACAGAAAGCGCGTT+TGG	0.332684	6.3:+79722421	MS.gene61422:CDS
ATTGATTAATGAAAGGGTTC+AGG	0.341931	6.3:+79722475	MS.gene61422:CDS
GAAGATGAGTCAGGGTGATT+TGG	0.359012	6.3:+79722448	MS.gene61422:CDS
AAGGTCTTGTGCTTTGGGTT+TGG	0.379299	6.3:-79722222	None:intergenic
AGCTTCCTCGCGTTTAAAAC+CGG	0.385062	6.3:-79722344	None:intergenic
GGGCTTGGTTTATGGCCTTT+GGG	0.397588	6.3:-79722250	None:intergenic
GGCGAAATTGATTAATGAAA+GGG	0.407053	6.3:+79722469	MS.gene61422:CDS
CTGAACCAGCAGCGTTGGAG+CGG	0.424927	6.3:+79722378	MS.gene61422:CDS
TAGGAACAATAAAACAAGAC+TGG	0.459948	6.3:+79722177	MS.gene61422:CDS
ATAAACCAAGCCCTCCGAAC+CGG	0.471149	6.3:+79722260	MS.gene61422:CDS
GCAGCGTTGGAGCGGGTTGG+TGG	0.474323	6.3:+79722386	MS.gene61422:CDS
TGGCGAAATTGATTAATGAA+AGG	0.476549	6.3:+79722468	MS.gene61422:CDS
TTTGGATAAGAAGATGAGTC+AGG	0.478923	6.3:+79722439	MS.gene61422:CDS
GGTAAAGCTGTTATTAATCA+AGG	0.485331	6.3:+79722518	MS.gene61422:CDS
AGCGGCTGAACCAGCAGCGT+TGG	0.495442	6.3:+79722373	MS.gene61422:CDS
GACTGTTAAGACCTCTGCTC+CGG	0.499262	6.3:-79722279	None:intergenic
TTAAGACCTCTGCTCCGGTT+CGG	0.505245	6.3:-79722274	None:intergenic
CCAACCCGCTCCAACGCTGC+TGG	0.509376	6.3:-79722383	None:intergenic
GACCTCTGCTCCGGTTCGGA+GGG	0.511130	6.3:-79722270	None:intergenic
TGAACCAGCAGCGTTGGAGC+GGG	0.514144	6.3:+79722379	MS.gene61422:CDS
ATTGTTCCTACTGTTCGTGT+AGG	0.519285	6.3:-79722164	None:intergenic
AGACCTCTGCTCCGGTTCGG+AGG	0.519624	6.3:-79722271	None:intergenic
TTTAAAACCGGTCCGCTCGT+CGG	0.530698	6.3:-79722332	None:intergenic
CGCGAGGAAGCTAGATGAAG+CGG	0.564479	6.3:+79722355	MS.gene61422:CDS
GTTGTTCAGGAGTATGAGAA+TGG	0.565499	6.3:+79722497	MS.gene61422:CDS
AGCCCTCCGAACCGGAGCAG+AGG	0.582656	6.3:+79722268	MS.gene61422:CDS
CCAGCAGCGTTGGAGCGGGT+TGG	0.583304	6.3:+79722383	MS.gene61422:CDS
TTATGGCCTTTGGGTTACGA+AGG	0.594530	6.3:-79722241	None:intergenic
AGGAACAATAAAACAAGACT+GGG	0.609753	6.3:+79722178	MS.gene61422:CDS
AGGATGCCTACACGAACAGT+AGG	0.625281	6.3:+79722158	None:intergenic
AAAACAAGACTGGGAACCAG+TGG	0.626445	6.3:+79722187	MS.gene61422:CDS
ACAGTCAAGAAACCAACAGC+CGG	0.641212	6.3:+79722296	MS.gene61422:CDS
TTGGATAAGAAGATGAGTCA+GGG	0.654305	6.3:+79722440	MS.gene61422:CDS
ACAAGACCTTCGTAACCCAA+AGG	0.679598	6.3:+79722235	MS.gene61422:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	GTTATTAATCAAGGTGTTTT+GGG	+	chr6.3:79722527-79722546	MS.gene61422:CDS	25.0%
!!!	TGTTATTAATCAAGGTGTTT+TGG	+	chr6.3:79722526-79722545	MS.gene61422:CDS	25.0%
!!!	TTATTAATCAAGGTGTTTTG+GGG	+	chr6.3:79722528-79722547	MS.gene61422:CDS	25.0%
	AGGAACAATAAAACAAGACT+GGG	+	chr6.3:79722178-79722197	MS.gene61422:CDS	30.0%
	ATTGATTAATGAAAGGGTTC+AGG	+	chr6.3:79722475-79722494	MS.gene61422:CDS	30.0%
	GGCGAAATTGATTAATGAAA+GGG	+	chr6.3:79722469-79722488	MS.gene61422:CDS	30.0%
	GGTAAAGCTGTTATTAATCA+AGG	+	chr6.3:79722518-79722537	MS.gene61422:CDS	30.0%
	GTTAAACTTAGGGGTAAAAT+TGG	+	chr6.3:79722572-79722591	MS.gene61422:CDS	30.0%
	TAGGAACAATAAAACAAGAC+TGG	+	chr6.3:79722177-79722196	MS.gene61422:CDS	30.0%
	TGGCGAAATTGATTAATGAA+AGG	+	chr6.3:79722468-79722487	MS.gene61422:CDS	30.0%
	TTGGATAAGAAGATGAGTCA+GGG	+	chr6.3:79722440-79722459	MS.gene61422:CDS	35.0%
	TTTGGATAAGAAGATGAGTC+AGG	+	chr6.3:79722439-79722458	MS.gene61422:CDS	35.0%
	ATTGTTCCTACTGTTCGTGT+AGG	-	chr6.3:79722167-79722186	None:intergenic	40.0%
!	GGAAAATGGAGAAGGTTTTG+GGG	+	chr6.3:79722549-79722568	MS.gene61422:CDS	40.0%
!	GGGAAAATGGAGAAGGTTTT+GGG	+	chr6.3:79722548-79722567	MS.gene61422:CDS	40.0%
!	GTTGTTCAGGAGTATGAGAA+TGG	+	chr6.3:79722497-79722516	MS.gene61422:CDS	40.0%
!	TTACGAAGGTCTTGTGCTTT+GGG	-	chr6.3:79722230-79722249	None:intergenic	40.0%
!!	GAAAATGGAGAAGGTTTTGG+GGG	+	chr6.3:79722550-79722569	MS.gene61422:CDS	40.0%
!!!	AGGTTTTGGGGGTTAAACTT+AGG	+	chr6.3:79722561-79722580	MS.gene61422:CDS	40.0%
!!!	GGTTTTGGGGGTTAAACTTA+GGG	+	chr6.3:79722562-79722581	MS.gene61422:CDS	40.0%
!!!	GTTTTGGGGGTTAAACTTAG+GGG	+	chr6.3:79722563-79722582	MS.gene61422:CDS	40.0%
!!!	TCAAGGTGTTTTGGGGAAAA+TGG	+	chr6.3:79722535-79722554	MS.gene61422:CDS	40.0%
!!!	TGTTTTGGGGAAAATGGAGA+AGG	+	chr6.3:79722541-79722560	MS.gene61422:CDS	40.0%
	AAAACAAGACTGGGAACCAG+TGG	+	chr6.3:79722187-79722206	MS.gene61422:CDS	45.0%
	ACAAGACCTTCGTAACCCAA+AGG	+	chr6.3:79722235-79722254	MS.gene61422:CDS	45.0%
	ACAGTCAAGAAACCAACAGC+CGG	+	chr6.3:79722296-79722315	MS.gene61422:CDS	45.0%
	GAAGATGAGTCAGGGTGATT+TGG	+	chr6.3:79722448-79722467	MS.gene61422:CDS	45.0%
	TCGAACAAAAAACCGACGAG+CGG	+	chr6.3:79722320-79722339	MS.gene61422:CDS	45.0%
!	AAGGTCTTGTGCTTTGGGTT+TGG	-	chr6.3:79722225-79722244	None:intergenic	45.0%
!	AGCTTCCTCGCGTTTAAAAC+CGG	-	chr6.3:79722347-79722366	None:intergenic	45.0%
!	GGGGAAAATGGAGAAGGTTT+TGG	+	chr6.3:79722547-79722566	MS.gene61422:CDS	45.0%
!	GTTACGAAGGTCTTGTGCTT+TGG	-	chr6.3:79722231-79722250	None:intergenic	45.0%
!	TTATGGCCTTTGGGTTACGA+AGG	-	chr6.3:79722244-79722263	None:intergenic	45.0%
!	TTTTTGTTCGAACCGGCTGT+TGG	-	chr6.3:79722311-79722330	None:intergenic	45.0%
!!	TGAAAGGGTTCAGGTTGTTC+AGG	+	chr6.3:79722484-79722503	MS.gene61422:CDS	45.0%
!!!	CGTCGGTTTTTTGTTCGAAC+CGG	-	chr6.3:79722318-79722337	None:intergenic	45.0%
!!!	GTGCTTTGGGTTTGGTTTTG+TGG	-	chr6.3:79722217-79722236	None:intergenic	45.0%
!!!	TTGGTTTTGTGGAGAACCAC+TGG	-	chr6.3:79722206-79722225	None:intergenic	45.0%
	ATAAACCAAGCCCTCCGAAC+CGG	+	chr6.3:79722260-79722279	MS.gene61422:CDS	50.0%
	GACTGTTAAGACCTCTGCTC+CGG	-	chr6.3:79722282-79722301	None:intergenic	50.0%
	TTAAGACCTCTGCTCCGGTT+CGG	-	chr6.3:79722277-79722296	None:intergenic	50.0%
!	AGGGCTTGGTTTATGGCCTT+TGG	-	chr6.3:79722254-79722273	None:intergenic	50.0%
!	GGGCTTGGTTTATGGCCTTT+GGG	-	chr6.3:79722253-79722272	None:intergenic	50.0%
!	TTTAAAACCGGTCCGCTCGT+CGG	-	chr6.3:79722335-79722354	None:intergenic	50.0%
	CAAAAAACCGACGAGCGGAC+CGG	+	chr6.3:79722325-79722344	MS.gene61422:CDS	55.0%
	CGCGAGGAAGCTAGATGAAG+CGG	+	chr6.3:79722355-79722374	MS.gene61422:CDS	55.0%
!	CGCGATACAGAAAGCGCGTT+TGG	+	chr6.3:79722421-79722440	MS.gene61422:CDS	55.0%
!	GGTTCGGAGGGCTTGGTTTA+TGG	-	chr6.3:79722261-79722280	None:intergenic	55.0%
!	CTGAACCAGCAGCGTTGGAG+CGG	+	chr6.3:79722378-79722397	MS.gene61422:CDS	60.0%
!	TGAACCAGCAGCGTTGGAGC+GGG	+	chr6.3:79722379-79722398	MS.gene61422:CDS	60.0%
!!!	GCGGACCGGTTTTAAACGCG+AGG	+	chr6.3:79722339-79722358	MS.gene61422:CDS	60.0%
	AGCGGCTGAACCAGCAGCGT+TGG	+	chr6.3:79722373-79722392	MS.gene61422:CDS	65.0%
!!	AGACCTCTGCTCCGGTTCGG+AGG	-	chr6.3:79722274-79722293	None:intergenic	65.0%
!!	GACCTCTGCTCCGGTTCGGA+GGG	-	chr6.3:79722273-79722292	None:intergenic	65.0%
	AGCCCTCCGAACCGGAGCAG+AGG	+	chr6.3:79722268-79722287	MS.gene61422:CDS	70.0%
	CCAACCCGCTCCAACGCTGC+TGG	-	chr6.3:79722386-79722405	None:intergenic	70.0%
!	CCAGCAGCGTTGGAGCGGGT+TGG	+	chr6.3:79722383-79722402	MS.gene61422:CDS	70.0%
!!	CTGCTCCGGTTCGGAGGGCT+TGG	-	chr6.3:79722268-79722287	None:intergenic	70.0%
!!	GCAGCGTTGGAGCGGGTTGG+TGG	+	chr6.3:79722386-79722405	MS.gene61422:CDS	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	79722161	79722601	79722161	ID=MS.gene61422
chr6.3	mRNA	79722161	79722601	79722161	ID=MS.gene61422.t1;Parent=MS.gene61422
chr6.3	exon	79722161	79722601	79722161	ID=MS.gene61422.t1.exon1;Parent=MS.gene61422.t1
chr6.3	CDS	79722161	79722601	79722161	ID=cds.MS.gene61422.t1;Parent=MS.gene61422.t1

Gene Sequence

>MS.gene61422

ATGCCTACACGAACAGTAGGAACAATAAAACAAGACTGGGAACCAGTGGTTCTCCACAAAACCAAACCCAAAGCACAAGACCTTCGTAACCCAAAGGCCATAAACCAAGCCCTCCGAACCGGAGCAGAGGTCTTAACAGTCAAGAAACCAACAGCCGGTTCGAACAAAAAACCGACGAGCGGACCGGTTTTAAACGCGAGGAAGCTAGATGAAGCGGCTGAACCAGCAGCGTTGGAGCGGGTTGGTGGAGAAGTGAGACACGCGATACAGAAAGCGCGTTTGGATAAGAAGATGAGTCAGGGTGATTTGGCGAAATTGATTAATGAAAGGGTTCAGGTTGTTCAGGAGTATGAGAATGGTAAAGCTGTTATTAATCAAGGTGTTTTGGGGAAAATGGAGAAGGTTTTGGGGGTTAAACTTAGGGGTAAAATTGGAAAATAA

Protein sequence

>MS.gene61422.t1

MPTRTVGTIKQDWEPVVLHKTKPKAQDLRNPKAINQALRTGAEVLTVKKPTAGSNKKPTSGPVLNARKLDEAAEPAALERVGGEVRHAIQKARLDKKMSQGDLAKLINERVQVVQEYENGKAVINQGVLGKMEKVLGVKLRGKIGK