AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene61947

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61947.t1 MTR_5g067350 97.983 347 7 0 1 347 1 347 0.0 697
MS.gene61947.t1 MTR_5g067350 97.059 272 8 0 1 272 1 272 0.0 535
MS.gene61947.t1 MTR_8g067530 63.551 321 102 9 32 347 46 356 9.08e-133 383
MS.gene61947.t1 MTR_5g030710 62.883 326 99 9 33 347 21 335 1.46e-128 371
MS.gene61947.t1 MTR_2g101500 49.284 349 123 11 29 347 2 326 1.04e-91 277
MS.gene61947.t1 MTR_6g488150 53.220 295 87 9 57 347 7 254 3.71e-88 266
MS.gene61947.t1 MTR_8g103030 47.273 330 104 9 50 347 2 293 6.73e-84 256
MS.gene61947.t1 MTR_1g080860 65.854 164 51 3 188 346 89 252 1.45e-69 218
MS.gene61947.t1 MTR_7g096090 65.823 158 52 2 191 347 94 250 1.89e-68 215
MS.gene61947.t1 MTR_1g070520 64.596 161 52 4 191 346 84 244 9.02e-67 211
MS.gene61947.t1 MTR_1g093240 62.264 159 58 2 191 347 78 236 1.42e-65 207
MS.gene61947.t1 MTR_1g080860 64.286 154 50 3 188 336 89 242 7.23e-63 201
MS.gene61947.t1 MTR_1g093240 61.905 147 54 2 191 335 78 224 8.08e-59 190
MS.gene61947.t1 MTR_1g070830 55.357 168 47 5 175 336 57 202 4.27e-50 166
MS.gene61947.t1 MTR_1g040675 51.515 165 56 4 172 336 43 183 1.36e-47 159
MS.gene61947.t1 MTR_1g093240 60.630 127 48 2 191 315 78 204 2.35e-47 159
MS.gene61947.t1 MTR_1g076270 53.503 157 46 6 180 335 48 178 8.59e-47 157
MS.gene61947.t1 MTR_1g093350 53.503 157 46 6 180 335 48 178 8.59e-47 157
MS.gene61947.t1 MTR_6g488150 45.161 217 71 8 57 272 7 176 2.25e-41 143
MS.gene61947.t1 MTR_1g040675 51.064 141 45 4 175 315 46 162 1.12e-37 132
MS.gene61947.t1 MTR_1g069495 43.478 184 69 5 195 343 81 264 4.24e-36 132
MS.gene61947.t1 MTR_4g011880 49.324 148 46 4 194 339 52 172 1.87e-35 127
MS.gene61947.t1 MTR_2g100780 40.217 184 78 6 182 338 85 263 1.49e-31 120
MS.gene61947.t1 MTR_2g100780 40.571 175 74 5 182 331 85 254 2.37e-31 119
MS.gene61947.t1 MTR_2g102490 54.902 102 37 3 232 330 88 183 2.43e-31 117
MS.gene61947.t1 MTR_8g014520 41.279 172 83 4 178 331 89 260 3.63e-31 119
MS.gene61947.t1 MTR_8g014520 41.279 172 83 4 178 331 89 260 3.92e-31 119
MS.gene61947.t1 MTR_4g124300 33.987 153 71 6 186 332 56 184 4.81e-19 84.0
MS.gene61947.t1 MTR_1g085750 40.741 108 48 3 230 335 83 176 2.95e-17 79.0
MS.gene61947.t1 MTR_5g057080 55.128 78 12 1 114 191 22 76 6.07e-17 75.9
MS.gene61947.t1 MTR_3g106850 31.469 143 72 5 196 332 88 210 2.58e-16 77.0
MS.gene61947.t1 MTR_5g043705 70.000 50 15 0 154 203 17 66 1.42e-15 71.6
MS.gene61947.t1 MTR_7g110790 38.384 99 50 2 230 328 68 155 1.48e-15 73.9
MS.gene61947.t1 MTR_1g109510 31.690 142 80 3 187 328 44 168 2.24e-15 73.6
MS.gene61947.t1 MTR_4g115075 31.343 134 71 4 197 329 93 206 1.72e-14 72.0
MS.gene61947.t1 MTR_1g085750 40.909 88 37 2 230 315 83 157 7.37e-12 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene61947.t1 AT2G22670 57.099 324 105 11 32 346 20 318 1.08e-109 323
MS.gene61947.t1 AT2G22670 57.099 324 105 11 32 346 20 318 1.08e-109 323
MS.gene61947.t1 AT5G65670 57.500 320 115 9 32 346 32 335 1.65e-109 323
MS.gene61947.t1 AT2G22670 57.099 324 105 11 32 346 37 335 1.72e-109 323
MS.gene61947.t1 AT5G65670 57.143 322 115 10 32 346 32 337 1.40e-107 318
MS.gene61947.t1 AT2G22670 56.481 324 105 12 32 346 20 316 2.16e-106 314
MS.gene61947.t1 AT2G22670 56.481 324 105 12 32 346 20 316 2.16e-106 314
MS.gene61947.t1 AT4G29080 48.348 333 119 9 33 347 8 305 3.36e-87 265
MS.gene61947.t1 AT4G14550 45.070 284 99 8 65 347 1 228 2.77e-66 209
MS.gene61947.t1 AT1G04250 42.160 287 96 6 65 346 7 228 8.62e-65 205
MS.gene61947.t1 AT3G04730 43.098 297 91 8 65 346 2 235 5.78e-63 201
MS.gene61947.t1 AT3G23050 43.902 287 97 9 65 342 6 237 1.33e-61 197
MS.gene61947.t1 AT4G14550 43.956 273 96 8 65 336 1 217 4.41e-60 193
MS.gene61947.t1 AT1G04240 53.548 155 54 4 182 336 50 186 3.31e-49 164
MS.gene61947.t1 AT1G04240 53.548 155 54 4 182 336 150 286 2.39e-48 164
MS.gene61947.t1 AT4G14550 42.231 251 88 8 65 314 1 195 1.14e-47 160
MS.gene61947.t1 AT5G43700 51.299 154 55 3 183 336 49 182 6.57e-46 155
MS.gene61947.t1 AT3G23050 41.154 260 89 9 65 315 6 210 1.97e-44 152
MS.gene61947.t1 AT3G23030 48.980 147 46 2 189 335 53 170 3.91e-41 142
MS.gene61947.t1 AT3G15540 49.359 156 54 5 190 342 64 197 1.78e-40 141
MS.gene61947.t1 AT3G23030 48.980 147 46 2 189 335 140 257 2.43e-40 143
MS.gene61947.t1 AT1G52830 45.333 150 57 4 187 336 61 185 1.17e-38 136
MS.gene61947.t1 AT2G33310 44.624 186 67 6 182 341 62 237 1.85e-38 137
MS.gene61947.t1 AT2G33310 44.624 186 66 7 182 341 62 236 7.39e-37 133
MS.gene61947.t1 AT2G33310 44.624 186 66 7 182 341 62 236 7.39e-37 133
MS.gene61947.t1 AT4G28640 46.914 162 66 4 192 336 84 242 1.42e-36 132
MS.gene61947.t1 AT4G28640 46.875 160 65 4 192 334 84 240 2.60e-36 132
MS.gene61947.t1 AT1G04550 42.857 196 80 6 175 347 50 236 4.56e-36 131
MS.gene61947.t1 AT1G15580 39.665 179 72 4 158 330 11 159 1.07e-35 127
MS.gene61947.t1 AT4G28640 46.875 160 65 4 192 334 84 240 1.77e-35 131
MS.gene61947.t1 AT4G14560 49.655 145 46 3 188 332 47 164 1.42e-34 125
MS.gene61947.t1 AT1G04100 39.884 173 71 5 193 335 90 259 4.20e-31 118
MS.gene61947.t1 AT3G16500 39.205 176 77 5 185 331 78 252 1.94e-30 117
MS.gene61947.t1 AT3G16500 39.205 176 77 5 185 331 78 252 2.05e-30 117
MS.gene61947.t1 AT1G51950 37.647 170 85 4 182 331 82 250 1.98e-29 114
MS.gene61947.t1 AT5G25890 36.686 169 87 3 170 338 22 170 2.27e-27 106
MS.gene61947.t1 AT4G14550 37.198 207 74 6 65 271 1 151 7.42e-27 105
MS.gene61947.t1 AT3G17600 39.375 160 68 8 175 328 18 154 4.82e-24 97.1
MS.gene61947.t1 AT3G23050 36.744 215 73 8 65 271 6 165 1.74e-23 95.5
MS.gene61947.t1 AT2G46990 40.385 104 50 1 227 330 81 172 1.57e-19 85.1
MS.gene61947.t1 AT3G62100 39.604 101 49 1 229 329 81 169 2.43e-19 84.7
MS.gene61947.t1 AT3G62100 39.080 87 41 1 229 315 81 155 4.50e-14 69.3
MS.gene61947.t1 AT1G15050 37.255 102 51 1 232 333 62 150 7.13e-14 68.9
MS.gene61947.t1 AT2G46990 39.080 87 41 1 229 315 83 157 7.19e-14 68.9
MS.gene61947.t1 AT1G15050 37.255 102 51 1 232 333 94 182 1.52e-13 68.9
MS.gene61947.t1 AT2G01200 34.906 106 56 1 228 333 96 188 8.28e-13 66.6
MS.gene61947.t1 AT2G01200 34.906 106 56 1 228 333 96 188 8.28e-13 66.6
MS.gene61947.t1 AT4G32280 32.653 98 49 2 231 328 118 198 2.60e-12 65.9
MS.gene61947.t1 AT4G32280 24.848 165 76 4 195 328 93 240 4.09e-12 65.9

Find 63 sgRNAs with CRISPR-Local

Find 174 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGAGGTTGATGGAAAAT+TGG 0.178401 5.2:+57326853 MS.gene61947:CDS
GGCAACATTCTCAAGCATTT+TGG 0.180467 5.2:-57326244 None:intergenic
AAATCTGATGAATCTCTTTC+AGG 0.213261 5.2:-57325847 None:intergenic
TTTAAGATTCAAATTACTTT+TGG 0.274939 5.2:-57325785 None:intergenic
GAAAGCAAACCAAGTCTTAA+TGG 0.296319 5.2:+57326336 MS.gene61947:CDS
TGCTTGTGGGTGATGTTCCC+TGG 0.320651 5.2:+57327227 MS.gene61947:CDS
AGCTCTGATGCCATTGGTTT+AGG 0.336962 5.2:+57328235 MS.gene61947:CDS
TAAAGCTACTGAACTTAGAT+TGG 0.345821 5.2:+57325804 MS.gene61947:CDS
GAACTTAGATTGGGACTTCC+AGG 0.346391 5.2:+57325814 MS.gene61947:CDS
TTGCTTTCACCAACACCATT+TGG 0.366646 5.2:-57326321 None:intergenic
CCCATGTAGTTAAATTCCTT+TGG 0.370283 5.2:-57325694 None:intergenic
CTTAATGGTTCTGCAACTAA+TGG 0.371208 5.2:+57326351 MS.gene61947:CDS
ATCCACTGATGAACAATTAG+AGG 0.372614 5.2:-57325725 None:intergenic
CTTTCAGGAGAAAGTGATCC+TGG 0.402526 5.2:-57325832 None:intergenic
GAAGAGGTTGATGGAAAATT+GGG 0.409009 5.2:+57326854 MS.gene61947:CDS
TGTGGCAGGGCACAGGTTGT+TGG 0.411315 5.2:+57326773 MS.gene61947:intron
AGAATATGTTATTACTTATG+AGG 0.411394 5.2:+57327186 MS.gene61947:CDS
TTCATTGTTCTTTGAAGCAG+TGG 0.422775 5.2:-57326834 None:intergenic
AAAATGCTTGAGAATGTTGC+CGG 0.428584 5.2:+57326246 MS.gene61947:CDS
AAGCTGAGGGATCTGCTTCA+TGG 0.432732 5.2:+57327160 MS.gene61947:CDS
GGCCTCCAATAAGATCGTTT+AGG 0.457342 5.2:+57326798 MS.gene61947:CDS
AAGAACAATGAAGAGGTTGA+TGG 0.459868 5.2:+57326845 MS.gene61947:CDS
TCATCACCTATTCCTAGCCT+TGG 0.471158 5.2:-57325613 None:intergenic
GAAATAGCAACAGTTAAGAT+TGG 0.473936 5.2:+57326288 MS.gene61947:CDS
GCAGGGCACAGGTTGTTGGT+TGG 0.475629 5.2:+57326777 MS.gene61947:intron
TCTCTACCAAGGCTAGGAAT+AGG 0.484039 5.2:+57325607 MS.gene61947:CDS
TTCCTAAACGATCTTATTGG+AGG 0.485580 5.2:-57326800 None:intergenic
GTTGATGGAAAATTGGGATC+AGG 0.490761 5.2:+57326860 MS.gene61947:CDS
AAAGCTACTGAACTTAGATT+GGG 0.491159 5.2:+57325805 MS.gene61947:CDS
ATTACTTATGAGGATAAAGA+TGG 0.497598 5.2:+57327196 MS.gene61947:CDS
GCTTGTGGGTGATGTTCCCT+GGG 0.507108 5.2:+57327228 MS.gene61947:CDS
ATTGGTCTTGAGAGGCCAAA+TGG 0.514099 5.2:+57326306 MS.gene61947:CDS
ATCAGCTTTCACTTTCTGCC+CGG 0.514822 5.2:-57326265 None:intergenic
ACCAAAGGAATTTAACTACA+TGG 0.515662 5.2:+57325693 MS.gene61947:CDS
ATGAGGATAAAGATGGTGAT+TGG 0.518002 5.2:+57327203 MS.gene61947:CDS
ATGAAGAGCTCTGATGCCAT+TGG 0.522246 5.2:+57328229 MS.gene61947:CDS
ATGTTCAGCTGTTTCACCAT+AGG 0.526626 5.2:+57326989 MS.gene61947:CDS
AATCTTGTGTGGCAGGGCAC+AGG 0.532182 5.2:+57326766 MS.gene61947:intron
CACCCTACTTACTTGCTAGC+AGG 0.532961 5.2:-57326387 None:intergenic
GATGGTGATTGGATGCTTGT+GGG 0.535428 5.2:+57327214 MS.gene61947:CDS
TTTGTGAAGGTCAGCATGGA+TGG 0.538161 5.2:+57326893 MS.gene61947:CDS
TTTGAATGGTTCAAAACCAA+AGG 0.539779 5.2:+57325678 MS.gene61947:CDS
GGATGTTTATTGATACATGC+AGG 0.544403 5.2:+57328194 MS.gene61947:intron
TCAGGTCAATGTGAATCTCA+TGG 0.548741 5.2:+57327115 MS.gene61947:intron
CCAAAGGAATTTAACTACAT+GGG 0.552535 5.2:+57325694 MS.gene61947:CDS
CAGTTAAGATTGGTCTTGAG+AGG 0.563647 5.2:+57326298 MS.gene61947:CDS
GTTGCAGAACCATTAAGACT+TGG 0.566322 5.2:-57326345 None:intergenic
ATGAAGCAGATCCCTCAGCT+TGG 0.567500 5.2:-57327158 None:intergenic
AGATGGTGATTGGATGCTTG+TGG 0.576179 5.2:+57327213 MS.gene61947:CDS
ATCCTCTAATTGTTCATCAG+TGG 0.580106 5.2:+57325723 MS.gene61947:CDS
GTGATGTCTCTACCAAGGCT+AGG 0.589347 5.2:+57325601 None:intergenic
AAATGCTTGAGAATGTTGCC+GGG 0.605417 5.2:+57326247 MS.gene61947:CDS
GGAATACTAACCTAAACCAA+TGG 0.612277 5.2:-57328245 None:intergenic
CTTGAGAGGCCAAATGGTGT+TGG 0.613950 5.2:+57326312 MS.gene61947:CDS
TGCTTCAAAGAACAATGAAG+AGG 0.621204 5.2:+57326838 MS.gene61947:CDS
TGCTGAATGAAACCAAGCTG+AGG 0.622855 5.2:+57327146 MS.gene61947:CDS
GCTGAATGAAACCAAGCTGA+GGG 0.626284 5.2:+57327147 MS.gene61947:CDS
AGTACATAAACTTACTCCCA+GGG 0.630905 5.2:-57327244 None:intergenic
GATGGAAAATTGGGATCAGG+AGG 0.633939 5.2:+57326863 MS.gene61947:CDS
AGGAGGTGCTGTGTTTGTGA+AGG 0.651647 5.2:+57326880 MS.gene61947:CDS
TCATTGTGATGTCTCTACCA+AGG 0.666331 5.2:+57325596 None:intergenic
TGTGTTTGTGAAGGTCAGCA+TGG 0.667221 5.2:+57326889 MS.gene61947:CDS
TGTTTATTGATACATGCAGG+AGG 0.676885 5.2:+57328197 MS.gene61947:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTACTTTTTTATAAAATAA+TGG + chr5.2:57326157-57326176 MS.gene61947:intron 10.0%
!!! TTTTTATCAAAAATAGAAAT+TGG - chr5.2:57328396-57328415 None:intergenic 10.0%
!! AAATTGTTAATTCAATCAAT+AGG + chr5.2:57326014-57326033 MS.gene61947:intron 15.0%
!! AATTGTTAATTCAATCAATA+GGG + chr5.2:57326015-57326034 MS.gene61947:intron 15.0%
!!! TCTTTAAAAGATTGAATTTT+TGG - chr5.2:57325760-57325779 None:intergenic 15.0%
!!! TTTAAGATTCAAATTACTTT+TGG - chr5.2:57325788-57325807 None:intergenic 15.0%
!! AAATCACAAAAATTGAAATG+CGG - chr5.2:57326580-57326599 None:intergenic 20.0%
!! AATGTGACTTCAAATTAAAA+TGG + chr5.2:57327754-57327773 MS.gene61947:intron 20.0%
!! ACTAAATGAATATGTTACAT+GGG + chr5.2:57327938-57327957 MS.gene61947:intron 20.0%
!! AGAATATGTTATTACTTATG+AGG + chr5.2:57327186-57327205 MS.gene61947:CDS 20.0%
!! ATGTAAGGTATATTAATAGA+AGG + chr5.2:57327636-57327655 MS.gene61947:intron 20.0%
!! TTCTATTAATATACCTTACA+TGG - chr5.2:57327637-57327656 None:intergenic 20.0%
!!! AAAGTAAATTAAGTTGAAGT+AGG - chr5.2:57328372-57328391 None:intergenic 20.0%
!!! CACATTTTATTATCTTATCA+CGG + chr5.2:57327466-57327485 MS.gene61947:intron 20.0%
!!! TAAAAACAATGTTACTTTGT+TGG + chr5.2:57325642-57325661 MS.gene61947:CDS 20.0%
!!! TTGATACGATTTTATTGAAA+GGG + chr5.2:57328042-57328061 MS.gene61947:intron 20.0%
!!! TTTGATACGATTTTATTGAA+AGG + chr5.2:57328041-57328060 MS.gene61947:intron 20.0%
! AAATCAAAGTGAAGTAAACA+AGG - chr5.2:57328119-57328138 None:intergenic 25.0%
! ATTACTTATGAGGATAAAGA+TGG + chr5.2:57327196-57327215 MS.gene61947:CDS 25.0%
! CACTAAATGAATATGTTACA+TGG + chr5.2:57327937-57327956 MS.gene61947:intron 25.0%
! CTAAATGAATATGTTACATG+GGG + chr5.2:57327939-57327958 MS.gene61947:intron 25.0%
! CTTAACAATGAAAATGAACT+CGG + chr5.2:57326729-57326748 MS.gene61947:intron 25.0%
! GATTGATTGTGTTAAATGAA+TGG - chr5.2:57328269-57328288 None:intergenic 25.0%
!! AAAAGATGATCATCTTTTTG+AGG - chr5.2:57325946-57325927 None:intergenic 25.0%
!! AAAGATGATCATCTTTTTGA+GGG - chr5.2:57325947-57325928 None:intergenic 25.0%
!! CACATAATTTGTACTTGAAT+TGG - chr5.2:57328452-57328471 None:intergenic 25.0%
!! TTTGAATTGGCTTAAATTGT+TGG - chr5.2:57327781-57327800 None:intergenic 25.0%
!! TTTTTCCTAAACGATCTTAT+TGG - chr5.2:57326806-57326825 None:intergenic 25.0%
!!! AAGTCTACTTTTCTCAAATA+CGG - chr5.2:57326923-57326942 None:intergenic 25.0%
!!! TACTGCAAGGTTTTAAATTT+AGG + chr5.2:57326431-57326450 MS.gene61947:intron 25.0%
!!! TTGTGGTTTTAGTTTGAAAT+AGG + chr5.2:57327607-57327626 MS.gene61947:intron 25.0%
AAAGCTACTGAACTTAGATT+GGG + chr5.2:57325805-57325824 MS.gene61947:CDS 30.0%
AAATCGTATCAAATTCGCTA+TGG - chr5.2:57328034-57328053 None:intergenic 30.0%
AAATCTGATGAATCTCTTTC+AGG - chr5.2:57325850-57325869 None:intergenic 30.0%
AATATATCTCAACTCTTTGC+AGG + chr5.2:57328173-57328192 MS.gene61947:intron 30.0%
ACCAAAGGAATTTAACTACA+TGG + chr5.2:57325693-57325712 MS.gene61947:CDS 30.0%
ACCATTATTTCGTCTAACTA+CGG - chr5.2:57327826-57327845 None:intergenic 30.0%
AGTTGAGATATATTAGGTGT+TGG - chr5.2:57328167-57328186 None:intergenic 30.0%
ATCACAGTTACTTACAAATG+CGG + chr5.2:57326505-57326524 MS.gene61947:intron 30.0%
ATGAGGAAAATTCTTAGAGA+GGG + chr5.2:57328065-57328084 MS.gene61947:intron 30.0%
ATGTTTGGAAATTGGAATCA+TGG - chr5.2:57327908-57327927 None:intergenic 30.0%
ATTCAATCAATAGGGTATGA+TGG + chr5.2:57326023-57326042 MS.gene61947:intron 30.0%
CAAGTACAAATTATGTGTTG+CGG + chr5.2:57328455-57328474 MS.gene61947:intron 30.0%
CAGTTGAAAAAAGTAAAAGC+AGG + chr5.2:57328499-57328518 MS.gene61947:CDS 30.0%
CCAAAGGAATTTAACTACAT+GGG + chr5.2:57325694-57325713 MS.gene61947:CDS 30.0%
GAAATAGCAACAGTTAAGAT+TGG + chr5.2:57326288-57326307 MS.gene61947:CDS 30.0%
GCAAAGAGTTGAGATATATT+AGG - chr5.2:57328173-57328192 None:intergenic 30.0%
GTCTTCAATAATGCCAAAAA+TGG - chr5.2:57327672-57327691 None:intergenic 30.0%
TAAAGCTACTGAACTTAGAT+TGG + chr5.2:57325804-57325823 MS.gene61947:CDS 30.0%
TGAAACGCAAAATTGAAAAC+AGG - chr5.2:57327982-57328001 None:intergenic 30.0%
TTTGAATGGTTCAAAACCAA+AGG + chr5.2:57325678-57325697 MS.gene61947:CDS 30.0%
TTTGTAAGTAACTGTGATTC+AGG - chr5.2:57326503-57326522 None:intergenic 30.0%
! ATACAAATTTTGCAGCATTG+TGG + chr5.2:57327590-57327609 MS.gene61947:intron 30.0%
! GAAAAAAGTGTGCATTTGAA+TGG + chr5.2:57325664-57325683 MS.gene61947:CDS 30.0%
! TACCTCTGAAAAAACATTCA+TGG - chr5.2:57325995-57326014 None:intergenic 30.0%
!! CTGTTTTAGGTAACAAAAGA+GGG + chr5.2:57325959-57325978 MS.gene61947:CDS 30.0%
!!! CTTTTTTTAGAAGCTTGGTT+TGG + chr5.2:57326706-57326725 MS.gene61947:intron 30.0%
!!! TGGTTTTAGTTTGAAATAGG+TGG + chr5.2:57327610-57327629 MS.gene61947:intron 30.0%
!!! TTACTTTTTTCAACTGCTCT+GGG - chr5.2:57328495-57328514 None:intergenic 30.0%
!!! TTTACTTTTTTCAACTGCTC+TGG - chr5.2:57328496-57328515 None:intergenic 30.0%
AAAATGCTTGAGAATGTTGC+CGG + chr5.2:57326246-57326265 MS.gene61947:CDS 35.0%
AAATTGCCTCAAATCGTTGA+AGG - chr5.2:57327056-57327075 None:intergenic 35.0%
AAGAACAATGAAGAGGTTGA+TGG + chr5.2:57326845-57326864 MS.gene61947:CDS 35.0%
AAGTACATAAACTTACTCCC+AGG - chr5.2:57327248-57327267 None:intergenic 35.0%
AATAGTCCTTCAACGATTTG+AGG + chr5.2:57327047-57327066 MS.gene61947:intron 35.0%
AGTACATAAACTTACTCCCA+GGG - chr5.2:57327247-57327266 None:intergenic 35.0%
ATCCACTGATGAACAATTAG+AGG - chr5.2:57325728-57325747 None:intergenic 35.0%
ATCCTCTAATTGTTCATCAG+TGG + chr5.2:57325723-57325742 MS.gene61947:CDS 35.0%
ATGACTGCATGTTTGGAAAT+TGG - chr5.2:57327916-57327935 None:intergenic 35.0%
ATGAGGATAAAGATGGTGAT+TGG + chr5.2:57327203-57327222 MS.gene61947:CDS 35.0%
CCCATGTAGTTAAATTCCTT+TGG - chr5.2:57325697-57325716 None:intergenic 35.0%
CTTGAATCACGATTGTATCA+CGG + chr5.2:57326632-57326651 MS.gene61947:intron 35.0%
GAAAGCAAACCAAGTCTTAA+TGG + chr5.2:57326336-57326355 MS.gene61947:CDS 35.0%
GATGAGGAAAATTCTTAGAG+AGG + chr5.2:57328064-57328083 MS.gene61947:intron 35.0%
GATGATTGATGAATCTTGTG+TGG + chr5.2:57326755-57326774 MS.gene61947:intron 35.0%
GCTTATGTTGATGTGATTTC+AGG + chr5.2:57327097-57327116 MS.gene61947:intron 35.0%
GGAATACTAACCTAAACCAA+TGG - chr5.2:57328248-57328267 None:intergenic 35.0%
GGATGTTTATTGATACATGC+AGG + chr5.2:57328194-57328213 MS.gene61947:intron 35.0%
TGCTTCAAAGAACAATGAAG+AGG + chr5.2:57326838-57326857 MS.gene61947:CDS 35.0%
TGGGAGTAAGTTTATGTACT+TGG + chr5.2:57327247-57327266 MS.gene61947:intron 35.0%
TGTTTATTGATACATGCAGG+AGG + chr5.2:57328197-57328216 MS.gene61947:CDS 35.0%
TTAGTGTATGACTGCATGTT+TGG - chr5.2:57327923-57327942 None:intergenic 35.0%
TTCCTAAACGATCTTATTGG+AGG - chr5.2:57326803-57326822 None:intergenic 35.0%
! CGATTTTATTGAAAGGGATG+AGG + chr5.2:57328048-57328067 MS.gene61947:intron 35.0%
! CTTAATGGTTCTGCAACTAA+TGG + chr5.2:57326351-57326370 MS.gene61947:CDS 35.0%
! CTTTTGTTACCTAAAACAGC+AGG - chr5.2:57325958-57325977 None:intergenic 35.0%
! TACAGCTCACTATTAAGTTG+CGG + chr5.2:57326106-57326125 MS.gene61947:intron 35.0%
!! GAAGAGGTTGATGGAAAATT+GGG + chr5.2:57326854-57326873 MS.gene61947:CDS 35.0%
!! GCTGTTTTAGGTAACAAAAG+AGG + chr5.2:57325958-57325977 MS.gene61947:CDS 35.0%
!! TGAAGAGGTTGATGGAAAAT+TGG + chr5.2:57326853-57326872 MS.gene61947:CDS 35.0%
!! TTCATTGTTCTTTGAAGCAG+TGG - chr5.2:57326837-57326856 None:intergenic 35.0%
!! TTTGAGGCAATTTTTGTGTC+TGG + chr5.2:57327063-57327082 MS.gene61947:intron 35.0%
!!! ACAAGCGCTAAGTTTTGAAT+TGG - chr5.2:57327794-57327813 None:intergenic 35.0%
!!! AGGTAAGAAACTGCCATTTT+TGG + chr5.2:57327656-57327675 MS.gene61947:intron 35.0%
!!! GCGAACTTTTTTTAGAAGCT+TGG + chr5.2:57326701-57326720 MS.gene61947:intron 35.0%
!!! GGTATGATGGTTTTAAACTG+CGG + chr5.2:57326036-57326055 MS.gene61947:intron 35.0%
!!! TACTTTTTTCAACTGCTCTG+GGG - chr5.2:57328494-57328513 None:intergenic 35.0%
!!! TGCCATGAATGTTTTTTCAG+AGG + chr5.2:57325990-57326009 MS.gene61947:CDS 35.0%
AAATGCTTGAGAATGTTGCC+GGG + chr5.2:57326247-57326266 MS.gene61947:CDS 40.0%
AACCTATTCCTACCTACCTA+TGG - chr5.2:57327008-57327027 None:intergenic 40.0%
AAGCTTGAATTGTACTGCGT+TGG + chr5.2:57327708-57327727 MS.gene61947:intron 40.0%
ATTGATGAATCTTGTGTGGC+AGG + chr5.2:57326759-57326778 MS.gene61947:intron 40.0%
CCCTCTGAATTGATTATGCA+GGG + chr5.2:57326204-57326223 MS.gene61947:intron 40.0%
CCCTGCATAATCAATTCAGA+GGG - chr5.2:57326207-57326226 None:intergenic 40.0%
CCTCTGAATTGATTATGCAG+GGG + chr5.2:57326205-57326224 MS.gene61947:intron 40.0%
CGATTAATGAAGCACAGACA+CGG + chr5.2:57327282-57327301 MS.gene61947:intron 40.0%
GAATATGTTACATGGGGCTT+AGG + chr5.2:57327945-57327964 MS.gene61947:intron 40.0%
GCCGTAGTTAGACGAAATAA+TGG + chr5.2:57327822-57327841 MS.gene61947:intron 40.0%
GTTGCAGAACCATTAAGACT+TGG - chr5.2:57326348-57326367 None:intergenic 40.0%
TCAGGTCAATGTGAATCTCA+TGG + chr5.2:57327115-57327134 MS.gene61947:intron 40.0%
TCCCTCTGAATTGATTATGC+AGG + chr5.2:57326203-57326222 MS.gene61947:intron 40.0%
TGAAATAGGTGGTCCATGTA+AGG + chr5.2:57327621-57327640 MS.gene61947:intron 40.0%
TGGCATTAGAGTTTACTGCA+AGG + chr5.2:57326418-57326437 MS.gene61947:intron 40.0%
TTGAATGTAACCACGTGTGT+TGG + chr5.2:57327356-57327375 MS.gene61947:intron 40.0%
TTGATGAATCTTGTGTGGCA+GGG + chr5.2:57326760-57326779 MS.gene61947:intron 40.0%
TTGCTTTCACCAACACCATT+TGG - chr5.2:57326324-57326343 None:intergenic 40.0%
! ATGTTCAGCTGTTTCACCAT+AGG + chr5.2:57326989-57327008 MS.gene61947:CDS 40.0%
! GACATCGTGGTTTTTGATGT+TGG - chr5.2:57326663-57326682 None:intergenic 40.0%
! GGCAACATTCTCAAGCATTT+TGG - chr5.2:57326247-57326266 None:intergenic 40.0%
! GTTGATGGAAAATTGGGATC+AGG + chr5.2:57326860-57326879 MS.gene61947:CDS 40.0%
!! AGGTGTTGGAATTAGTTTGC+AGG - chr5.2:57328153-57328172 None:intergenic 40.0%
!! CAGTTAAGATTGGTCTTGAG+AGG + chr5.2:57326298-57326317 MS.gene61947:CDS 40.0%
!! GGTGTTGGAATTAGTTTGCA+GGG - chr5.2:57328152-57328171 None:intergenic 40.0%
!! TCATCTTTTTGAGGGTGCAA+AGG - chr5.2:57325916-57325935 None:intergenic 40.0%
!!! ACTTTTTTCAACTGCTCTGG+GGG - chr5.2:57328493-57328512 None:intergenic 40.0%
!!! GAATCAAAGCCTGCTGTTTT+AGG + chr5.2:57325946-57325965 MS.gene61947:CDS 40.0%
!!! TTCTCAAGCATTTTGGAGCT+AGG - chr5.2:57326240-57326259 None:intergenic 40.0%
AAAAGTTCGCAACCGAAGTC+TGG - chr5.2:57326691-57326710 None:intergenic 45.0%
AAAGTTCGCAACCGAAGTCT+GGG - chr5.2:57326690-57326709 None:intergenic 45.0%
AGATGGTGATTGGATGCTTG+TGG + chr5.2:57327213-57327232 MS.gene61947:CDS 45.0%
ATCAGCTTTCACTTTCTGCC+CGG - chr5.2:57326268-57326287 None:intergenic 45.0%
CACCATAGGTAGGTAGGAAT+AGG + chr5.2:57327003-57327022 MS.gene61947:intron 45.0%
CATCAAAAACCACGATGTCG+CGG + chr5.2:57326664-57326683 MS.gene61947:intron 45.0%
CCCCTGCATAATCAATTCAG+AGG - chr5.2:57326208-57326227 None:intergenic 45.0%
CTCAGATTGCGGTCAAATAG+TGG + chr5.2:57326554-57326573 MS.gene61947:intron 45.0%
CTCCTGCTAGCAAGTAAGTA+GGG + chr5.2:57326385-57326404 MS.gene61947:intron 45.0%
CTGTTTCACCATAGGTAGGT+AGG + chr5.2:57326997-57327016 MS.gene61947:intron 45.0%
CTTTCAGGAGAAAGTGATCC+TGG - chr5.2:57325835-57325854 None:intergenic 45.0%
GAACTTAGATTGGGACTTCC+AGG + chr5.2:57325814-57325833 MS.gene61947:CDS 45.0%
GATGGAAAATTGGGATCAGG+AGG + chr5.2:57326863-57326882 MS.gene61947:CDS 45.0%
GATGGTGATTGGATGCTTGT+GGG + chr5.2:57327214-57327233 MS.gene61947:CDS 45.0%
GCTGAATGAAACCAAGCTGA+GGG + chr5.2:57327147-57327166 MS.gene61947:CDS 45.0%
GGCCTCCAATAAGATCGTTT+AGG + chr5.2:57326798-57326817 MS.gene61947:CDS 45.0%
GTAAGTAGGGTGGTGTGAAT+TGG + chr5.2:57326398-57326417 MS.gene61947:intron 45.0%
TCATCACCTATTCCTAGCCT+TGG - chr5.2:57325616-57325635 None:intergenic 45.0%
TCTCTACCAAGGCTAGGAAT+AGG + chr5.2:57325607-57325626 MS.gene61947:CDS 45.0%
TGCTGAATGAAACCAAGCTG+AGG + chr5.2:57327146-57327165 MS.gene61947:CDS 45.0%
TGTGTTTGTGAAGGTCAGCA+TGG + chr5.2:57326889-57326908 MS.gene61947:CDS 45.0%
TTTGTGAAGGTCAGCATGGA+TGG + chr5.2:57326893-57326912 MS.gene61947:CDS 45.0%
! ATGAAGAGCTCTGATGCCAT+TGG + chr5.2:57328229-57328248 MS.gene61947:CDS 45.0%
! TCAGCTGTTTCACCATAGGT+AGG + chr5.2:57326993-57327012 MS.gene61947:intron 45.0%
! TGAGGGTGCAAAGGAAAAAG+AGG - chr5.2:57325907-57325926 None:intergenic 45.0%
!! AGCTCTGATGCCATTGGTTT+AGG + chr5.2:57328235-57328254 MS.gene61947:CDS 45.0%
!! ATTGGTCTTGAGAGGCCAAA+TGG + chr5.2:57326306-57326325 MS.gene61947:CDS 45.0%
AAGCTGAGGGATCTGCTTCA+TGG + chr5.2:57327160-57327179 MS.gene61947:CDS 50.0%
ATGAAGCAGATCCCTCAGCT+TGG - chr5.2:57327161-57327180 None:intergenic 50.0%
CAATCTGAGCTGCGACATCA+TGG - chr5.2:57326543-57326562 None:intergenic 50.0%
CTGCTAGCAAGTAAGTAGGG+TGG + chr5.2:57326388-57326407 MS.gene61947:intron 50.0%
CTTGAGAGGCCAAATGGTGT+TGG + chr5.2:57326312-57326331 MS.gene61947:CDS 50.0%
GACACTGACATCGTCGACAT+TGG + chr5.2:57327309-57327328 MS.gene61947:intron 50.0%
GCTCCTGCTAGCAAGTAAGT+AGG + chr5.2:57326384-57326403 MS.gene61947:intron 50.0%
TGATGTCGCAGCTCAGATTG+CGG + chr5.2:57326543-57326562 MS.gene61947:intron 50.0%
! CACCCTACTTACTTGCTAGC+AGG - chr5.2:57326390-57326409 None:intergenic 50.0%
!! AGGAGGTGCTGTGTTTGTGA+AGG + chr5.2:57326880-57326899 MS.gene61947:CDS 50.0%
!! CACGTGTGTTGGTGTTGTGT+CGG + chr5.2:57327367-57327386 MS.gene61947:intron 50.0%
AATCTTGTGTGGCAGGGCAC+AGG + chr5.2:57326766-57326785 MS.gene61947:intron 55.0%
CGACACAACACCAACACACG+TGG - chr5.2:57327369-57327388 None:intergenic 55.0%
GCTTGTGGGTGATGTTCCCT+GGG + chr5.2:57327228-57327247 MS.gene61947:CDS 55.0%
TGCTTGTGGGTGATGTTCCC+TGG + chr5.2:57327227-57327246 MS.gene61947:CDS 55.0%
GCAGGGCACAGGTTGTTGGT+TGG + chr5.2:57326777-57326796 MS.gene61947:intron 60.0%
TGTGGCAGGGCACAGGTTGT+TGG + chr5.2:57326773-57326792 MS.gene61947:intron 60.0%
!! CTGCTCTGGGGGCTGCAAAA+TGG - chr5.2:57328482-57328501 None:intergenic 60.0%
AAGTCTGGGCCGCGACATCG+TGG - chr5.2:57326676-57326695 None:intergenic 65.0%
CGATGTCGCGGCCCAGACTT+CGG + chr5.2:57326676-57326695 MS.gene61947:intron 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 57325604 57328527 57325604 ID=MS.gene61947
chr5.2 mRNA 57325604 57328527 57325604 ID=MS.gene61947.t1;Parent=MS.gene61947
chr5.2 exon 57325604 57326011 57325604 ID=MS.gene61947.t1.exon1;Parent=MS.gene61947.t1
chr5.2 CDS 57325604 57326011 57325604 ID=cds.MS.gene61947.t1;Parent=MS.gene61947.t1
chr5.2 exon 57326225 57326397 57326225 ID=MS.gene61947.t1.exon2;Parent=MS.gene61947.t1
chr5.2 CDS 57326225 57326397 57326225 ID=cds.MS.gene61947.t1;Parent=MS.gene61947.t1
chr5.2 exon 57326781 57327010 57326781 ID=MS.gene61947.t1.exon3;Parent=MS.gene61947.t1
chr5.2 CDS 57326781 57327010 57326781 ID=cds.MS.gene61947.t1;Parent=MS.gene61947.t1
chr5.2 exon 57327119 57327251 57327119 ID=MS.gene61947.t1.exon4;Parent=MS.gene61947.t1
chr5.2 CDS 57327119 57327251 57327119 ID=cds.MS.gene61947.t1;Parent=MS.gene61947.t1
chr5.2 exon 57328195 57328256 57328195 ID=MS.gene61947.t1.exon5;Parent=MS.gene61947.t1
chr5.2 CDS 57328195 57328256 57328195 ID=cds.MS.gene61947.t1;Parent=MS.gene61947.t1
chr5.2 exon 57328490 57328527 57328490 ID=MS.gene61947.t1.exon6;Parent=MS.gene61947.t1
chr5.2 CDS 57328490 57328527 57328490 ID=cds.MS.gene61947.t1;Parent=MS.gene61947.t1
Gene Sequence

>MS.gene61947

ATGTCTCTACCAAGGCTAGGAATAGGTGATGAAGAAAGTAAAAACAATGTTACTTTGTTGGAAAAAAGTGTGCATTTGAATGGTTCAAAACCAAAGGAATTTAACTACATGGGATTATCATCCTCTAATTGTTCATCAGTGGATAGTTCAGTTCCAAAAATTCAATCTTTTAAAGATGAGACCAAAAGTAATTTGAATCTTAAAGCTACTGAACTTAGATTGGGACTTCCAGGATCACTTTCTCCTGAAAGAGATTCATCAGATTTTTGTTTGAGAAGTTCAAAGCAGTTTGATGAAAAGCCTCTTTTTCCTTTGCACCCTCAAAAAGATGATCATCTTTTTGAATCAAAGCCTGCTGTTTTAGGTAACAAAAGAGGGTTTTCTGATGCCATGAATGTTTTTTCAGAGGTAAATTGTTAATTCAATCAATAGGGTATGATGGTTTTAAACTGCGGTTGAAGCTATGCTGCGAACTTTTATATTGCAGTAAAAATGCTGTTGATACAGCTCACTATTAAGTTGCGGACTGCAATTTAGAACTATCTATGATCTATCTACTTTTTTATAAAATAATGGAAGATCGTACTAAGTATTAAAGTTCCCTCTGAATTGATTATGCAGGGGAAATTGAAGCCTAGCTCCAAAATGCTTGAGAATGTTGCCGGGCAGAAAGTGAAAGCTGATGAAATAGCAACAGTTAAGATTGGTCTTGAGAGGCCAAATGGTGTTGGTGAAAGCAAACCAAGTCTTAATGGTTCTGCAACTAATGGAAATAGCACTGCTCCTGCTAGCAAGTAAGTAGGGTGGTGTGAATTGGCATTAGAGTTTACTGCAAGGTTTTAAATTTAGGACATGTTACGTTTTTTGATACTGCGAATCAAATGCTGTTGATGCAGCCTGAATCACAGTTACTTACAAATGCGGCGACATCAAAAGCCATGATGTCGCAGCTCAGATTGCGGTCAAATAGTGGCCGCATTTCAATTTTTGTGATTTTTGAGAGTCATAGTCAAATGCAGCTGATGCAGCTTGAATCACGATTGTATCACGGTCATGCCAACATCAAAAACCACGATGTCGCGGCCCAGACTTCGGTTGCGAACTTTTTTTAGAAGCTTGGTTTGGCTTAACAATGAAAATGAACTCGGAATGATGATTGATGAATCTTGTGTGGCAGGGCACAGGTTGTTGGTTGGCCTCCAATAAGATCGTTTAGGAAAAATTCATTGACCACTGCTTCAAAGAACAATGAAGAGGTTGATGGAAAATTGGGATCAGGAGGTGCTGTGTTTGTGAAGGTCAGCATGGATGGTGCTCCGTATTTGAGAAAAGTAGACTTGAAGAATTACACTGCATATTCACAACTATCTTCTTCTCTTGAGAAGATGTTCAGCTGTTTCACCATAGGTAGGTAGGAATAGGTTCATTTTTGAATAAAAGAAAAATAGTCCTTCAACGATTTGAGGCAATTTTTGTGTCTGGATCAAGTTACTGCTTATGTTGATGTGATTTCAGGTCAATGTGAATCTCATGGAAATCAGATGCTGAATGAAACCAAGCTGAGGGATCTGCTTCATGGTTCAGAATATGTTATTACTTATGAGGATAAAGATGGTGATTGGATGCTTGTGGGTGATGTTCCCTGGGAGTAAGTTTATGTACTTGGTTTTCTTTCATGCGATTAATGAAGCACAGACACGGATATGACACTGACATCGTCGACATTGGTAGTATGTTTTGAAAATAGAATAATTGAATGTAACCACGTGTGTTGGTGTTGTGTCGGCACACATTCAATCTACATCTACATAAGTATACATCATCAACATTCTACGTAGATAAGTTTTAGACAAACGATTAATCACATTTTATTATCTTATCACGGCGTAAACACATAATGAAGTAGTTAAACTTATGTGTAAAATTTATTTATACAGTCTGATTATGCTGATAAAAATAATGAGTTGTTTTAAAATAATTAGAGAAATACAAATTTTGCAGCATTGTGGTTTTAGTTTGAAATAGGTGGTCCATGTAAGGTATATTAATAGAAGGTAAGAAACTGCCATTTTTGGCATTATTGAAGACTGTACAAAAGCAATAAAAGCTTGAATTGTACTGCGTTGGTGACATGTGAGTATAATCAGAACAATGTGACTTCAAATTAAAATGGACCAACAATTTAAGCCAATTCAAAACTTAGCGCTTGTTTAGATTAGCCGTAGTTAGACGAAATAATGGTGTAGCACGATAATTTTGACATAAGTTACACTTTAGAGCTTAAACAAAATCGCAGTGCTACCATGATTCCAATTTCCAAACATGCAGTCATACACTAAATGAATATGTTACATGGGGCTTAGGATTTTGATTATCCTGTTTTCAATTTTGCGTTTCATAGTATTTATTGCTTATTTCGTAAATAATCCATAGCGAATTTGATACGATTTTATTGAAAGGGATGAGGAAAATTCTTAGAGAGGGTCTATGTTTATAAACAAAGTTTTACTAACCTTGTTTACTTCACTTTGATTTTATATCTGCACCCTGCAAACTAATTCCAACACCTAATATATCTCAACTCTTTGCAGGATGTTTATTGATACATGCAGGAGGCTAAGAATCATGAAGAGCTCTGATGCCATTGGTTTAGGTTAGTATTCCATTCATTTAACACAATCAATCTTAAAAATTGTATTGCATATATATACTAAATGATCTTAAAAAAAAGAGATATTTGTTCACTTATCACAAAATAACAGAATCCTACTTCAACTTAATTTACTTTGCCAATTTCTATTTTTGATAAAAAAAATTATATATATTATACTATATGTTAGCATTTCCAATTCAAGTACAAATTATGTGTTGCGGTCCATTTTGCAGCCCCCAGAGCAGTTGAAAAAAGTAAAAGCAGGAACTAA
Protein sequence

>MS.gene61947.t1

MSLPRLGIGDEESKNNVTLLEKSVHLNGSKPKEFNYMGLSSSNCSSVDSSVPKIQSFKDETKSNLNLKATELRLGLPGSLSPERDSSDFCLRSSKQFDEKPLFPLHPQKDDHLFESKPAVLGNKRGFSDAMNVFSEGKLKPSSKMLENVAGQKVKADEIATVKIGLERPNGVGESKPSLNGSATNGNSTAPASKAQVVGWPPIRSFRKNSLTTASKNNEEVDGKLGSGGAVFVKVSMDGAPYLRKVDLKNYTAYSQLSSSLEKMFSCFTIGQCESHGNQMLNETKLRDLLHGSEYVITYEDKDGDWMLVGDVPWEMFIDTCRRLRIMKSSDAIGLAPRAVEKSKSRN