AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055331


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055331.t1 MTR_1g073320 100.000 172 0 0 1 172 1 172 1.31e-122 352
MS.gene055331.t1 MTR_1g073320 49.045 157 41 3 87 240 191 311 3.20e-19 85.5
MS.gene055331.t1 MTR_1g044580 52.397 292 73 5 1 228 1 290 3.15e-80 254
MS.gene055331.t1 MTR_7g098410 45.408 196 96 4 11 200 10 200 2.49e-46 155
MS.gene055331.t1 MTR_1g080350 40.351 171 90 6 12 172 10 178 5.14e-32 119
MS.gene055331.t1 MTR_5g078030 35.915 142 88 2 9 148 4 144 1.78e-21 90.5
MS.gene055331.t1 MTR_3g065370 31.724 145 90 3 6 148 5 142 1.86e-17 79.7
MS.gene055331.t1 MTR_8g104990 27.815 151 106 2 26 174 18 167 3.63e-12 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055331.t1 AT1G04520 62.500 184 65 1 8 191 10 189 2.69e-74 228
MS.gene055331.t1 AT1G04520 29.949 197 99 5 33 227 144 303 1.40e-12 66.2
MS.gene055331.t1 AT2G33330 62.778 180 62 3 1 175 1 180 7.87e-73 224
MS.gene055331.t1 AT2G33330 30.612 196 94 4 33 227 146 300 1.41e-11 63.5
MS.gene055331.t1 AT5G43980 47.097 155 75 2 25 172 20 174 2.91e-38 135
MS.gene055331.t1 AT3G04370 33.880 183 90 7 24 175 25 207 3.12e-22 93.6
MS.gene055331.t1 AT3G04370 31.868 182 94 5 24 175 25 206 4.63e-22 92.8
MS.gene055331.t1 AT5G37660 31.522 184 122 3 11 192 15 196 6.92e-22 92.0
MS.gene055331.t1 AT5G37660 31.522 184 122 3 11 192 15 196 6.98e-22 92.0
MS.gene055331.t1 AT3G60720 25.794 252 127 5 34 226 30 280 6.01e-17 78.6
MS.gene055331.t1 AT3G60720 25.498 251 127 5 33 224 29 278 6.13e-16 75.5
MS.gene055331.t1 AT3G60720 26.570 207 130 6 33 221 29 231 6.51e-15 72.4
MS.gene055331.t1 AT1G70690 32.168 143 91 4 10 148 9 149 8.81e-15 72.4
MS.gene055331.t1 AT2G01660 30.978 184 110 6 26 201 20 194 1.03e-13 68.9
MS.gene055331.t1 AT2G01660 30.978 184 110 6 26 201 20 194 1.78e-13 68.9
MS.gene055331.t1 AT2G01660 30.978 184 110 6 26 201 20 194 1.96e-13 68.6

Find 40 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGCAAGAGTTCAGCTTTC+AGG 0.163538 1.3:-52809333 MS.gene055331:CDS
TGAAGAACAAAAGAAGAAAA+AGG 0.259401 1.3:+52809661 None:intergenic
TCTGGTTACTCTTCATCTTT+TGG 0.262866 1.3:-52808919 MS.gene055331:CDS
AAGACTGTGGCAATAATATT+AGG 0.324267 1.3:-52808410 MS.gene055331:CDS
AGATACCTGTGATGCTGTTT+TGG 0.333639 1.3:+52809448 None:intergenic
GGTGGTGGATCATCCTCTTC+TGG 0.358061 1.3:-52808937 MS.gene055331:CDS
TAGACCAAAGTTGTGTAATC+AGG 0.371990 1.3:+52809600 None:intergenic
AAGATGATGATTTAGAGAAA+TGG 0.375671 1.3:+52809625 None:intergenic
GATTGTGAATATGCTCCATT+TGG 0.394933 1.3:+52809546 None:intergenic
AGATGATGATTTAGAGAAAT+GGG 0.405156 1.3:+52809626 None:intergenic
AAGGGTGGTCACTCTTCATC+TGG 0.416746 1.3:-52808958 MS.gene055331:CDS
TTCTCCTGATTACACAACTT+TGG 0.429549 1.3:-52809604 MS.gene055331:CDS
AGGTTCTTCAAAACAACAAC+TGG 0.461798 1.3:-52809477 MS.gene055331:CDS
GGTAGGTGTTGTGTTGTACA+TGG 0.467781 1.3:-52808317 MS.gene055331:CDS
ACAACAAATGCTGCTGGAAG+AGG 0.468669 1.3:-52809237 MS.gene055331:CDS
TACATGCTGTACGAGGTTGC+TGG 0.468867 1.3:-52809306 MS.gene055331:CDS
AAGCTGAACTCTTGCAGAGA+TGG 0.484956 1.3:+52809338 None:intergenic
CAGATTCTCTTCAAGACTTG+TGG 0.485266 1.3:-52809261 MS.gene055331:CDS
GGTCACTCTTCATCTGGTGG+TGG 0.489663 1.3:-52808952 MS.gene055331:CDS
TTCAAAACAACAACTGGTAA+TGG 0.498720 1.3:-52809471 MS.gene055331:CDS
CATAGTTTGTCTGAGAGAAC+TGG 0.504524 1.3:+52809369 None:intergenic
GAAACATTCACAGATCCAAA+TGG 0.507372 1.3:-52809561 MS.gene055331:CDS
TGTGGAACAACAAATGCTGC+TGG 0.508236 1.3:-52809243 MS.gene055331:CDS
TTGTGAGATCTCCTCTGCAT+TGG 0.514398 1.3:+52809424 None:intergenic
AATGGCCAAAACAGCATCAC+AGG 0.526879 1.3:-52809453 MS.gene055331:CDS
GCTATGACAGGACAAAATAC+AGG 0.532912 1.3:-52808434 MS.gene055331:intron
ATCTGCATCCCTGAAACTTG+TGG 0.533086 1.3:+52809279 None:intergenic
TTATATGCTTGCTATTTGCA+AGG 0.544026 1.3:-52808361 MS.gene055331:CDS
AGATGAAGAGTAACCAGAAG+AGG 0.557396 1.3:+52808924 None:intergenic
TACACAACTTTGGTCTACAA+AGG 0.569043 1.3:-52809594 MS.gene055331:CDS
GGTGGTCACTCTTCATCTGG+TGG 0.573580 1.3:-52808955 MS.gene055331:CDS
CAACAAATGCTGCTGGAAGA+GGG 0.576463 1.3:-52809236 MS.gene055331:CDS
ATTAGGAGGTATAGCTGCTG+TGG 0.619016 1.3:-52808393 MS.gene055331:CDS
ACAGGTATCTTCCAATGCAG+AGG 0.638244 1.3:-52809435 MS.gene055331:CDS
ACTGTGGCAATAATATTAGG+AGG 0.641949 1.3:-52808407 MS.gene055331:CDS
TGATGAAGAAACGTGATGGT+AGG 0.645756 1.3:-52808334 MS.gene055331:CDS
AGGTTGTTACATGCTGTACG+AGG 0.659964 1.3:-52809313 MS.gene055331:CDS
AATTTGATGAAGAAACGTGA+TGG 0.662013 1.3:-52808338 MS.gene055331:CDS
GTTCTCTCAGACAAACTATG+TGG 0.679766 1.3:-52809366 MS.gene055331:CDS
ACAAAATACAGGAAAGACTG+TGG 0.709113 1.3:-52808423 MS.gene055331:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTTCATACATATTAGTAAA+TGG + chr1.3:52809489-52809508 None:intergenic 20.0%
!! TTATAACACATGAACAATTA+AGG + chr1.3:52809435-52809454 None:intergenic 20.0%
!!! AGTTTTAGTTATTATCCTAA+TGG - chr1.3:52808991-52809010 MS.gene055331:intron 20.0%
!!! CAATTTTCTCAAATTATGAT+CGG + chr1.3:52809248-52809267 None:intergenic 20.0%
!!! GTTTTAGTTATTATCCTAAT+GGG - chr1.3:52808992-52809011 MS.gene055331:intron 20.0%
!!! TTTTACTTTTATGTTGAATC+TGG - chr1.3:52809166-52809185 MS.gene055331:intron 20.0%
!!! TTTTAGTTATTATCCTAATG+GGG - chr1.3:52808993-52809012 MS.gene055331:intron 20.0%
! AAGAACAAAAGAAGAAAAAG+GGG + chr1.3:52808319-52808338 None:intergenic 25.0%
! AAGATGATGATTTAGAGAAA+TGG + chr1.3:52808357-52808376 None:intergenic 25.0%
! AGATGATGATTTAGAGAAAT+GGG + chr1.3:52808356-52808375 None:intergenic 25.0%
! GAAGAACAAAAGAAGAAAAA+GGG + chr1.3:52808320-52808339 None:intergenic 25.0%
! TCAGAATCAATAGATAAGAT+TGG - chr1.3:52809107-52809126 MS.gene055331:intron 25.0%
! TGAAGAACAAAAGAAGAAAA+AGG + chr1.3:52808321-52808340 None:intergenic 25.0%
! TTTGAGAAAATTGAATCGAA+TGG - chr1.3:52809255-52809274 MS.gene055331:CDS 25.0%
!!! ATAAGATTGGTTTCAATTCT+GGG - chr1.3:52809120-52809139 MS.gene055331:intron 25.0%
!!! TATCTATTGATTCTGAATGA+TGG + chr1.3:52809103-52809122 None:intergenic 25.0%
!!! TTGTACATGGTTTTACTATA+TGG - chr1.3:52809675-52809694 MS.gene055331:CDS 25.0%
AAGACTGTGGCAATAATATT+AGG - chr1.3:52809569-52809588 MS.gene055331:CDS 30.0%
AATTTGATGAAGAAACGTGA+TGG - chr1.3:52809641-52809660 MS.gene055331:CDS 30.0%
TTCAAAACAACAACTGGTAA+TGG - chr1.3:52808508-52808527 MS.gene055331:intron 30.0%
TTTCACAATCCACAAAAACA+AGG - chr1.3:52808482-52808501 MS.gene055331:intron 30.0%
! ATTAATCATTGAGTTGTGTG+TGG - chr1.3:52809513-52809532 MS.gene055331:CDS 30.0%
! TATCAAGCTTTGTATGTGAT+GGG - chr1.3:52808844-52808863 MS.gene055331:intron 30.0%
! TTATCAAGCTTTGTATGTGA+TGG - chr1.3:52808843-52808862 MS.gene055331:intron 30.0%
!! GATAAGATTGGTTTCAATTC+TGG - chr1.3:52809119-52809138 MS.gene055331:intron 30.0%
!! TTATATGCTTGCTATTTGCA+AGG - chr1.3:52809618-52809637 MS.gene055331:CDS 30.0%
!! TTTTGTGGATTGTGAAACTA+AGG + chr1.3:52808479-52808498 None:intergenic 30.0%
!!! AGAAGAAAAAGGGGTTTTTT+TGG + chr1.3:52808310-52808329 None:intergenic 30.0%
!!! TTTGAAGAACCTTGTTTTTG+TGG + chr1.3:52808494-52808513 None:intergenic 30.0%
ACAAAATACAGGAAAGACTG+TGG - chr1.3:52809556-52809575 MS.gene055331:CDS 35.0%
ACCTTAACACTTCAGATTGA+AGG + chr1.3:52809361-52809380 None:intergenic 35.0%
ACTGTGGCAATAATATTAGG+AGG - chr1.3:52809572-52809591 MS.gene055331:CDS 35.0%
AGGTTCTTCAAAACAACAAC+TGG - chr1.3:52808502-52808521 MS.gene055331:intron 35.0%
GAAACATTCACAGATCCAAA+TGG - chr1.3:52808418-52808437 MS.gene055331:CDS 35.0%
GAAGTAGCTCAATTTAGCAA+TGG - chr1.3:52809399-52809418 MS.gene055331:CDS 35.0%
GAATGTGGAAGTTCTATTTC+AGG - chr1.3:52808940-52808959 MS.gene055331:CDS 35.0%
GATTGTGAATATGCTCCATT+TGG + chr1.3:52808436-52808455 None:intergenic 35.0%
TACACAACTTTGGTCTACAA+AGG - chr1.3:52808385-52808404 MS.gene055331:CDS 35.0%
TAGACCAAAGTTGTGTAATC+AGG + chr1.3:52808382-52808401 None:intergenic 35.0%
TTCTCCTGATTACACAACTT+TGG - chr1.3:52808375-52808394 MS.gene055331:CDS 35.0%
! ACCTTCAATCTGAAGTGTTA+AGG - chr1.3:52809357-52809376 MS.gene055331:CDS 35.0%
! CAATCTCTTTCAGCACTTTT+TGG - chr1.3:52808451-52808470 MS.gene055331:intron 35.0%
! TCTGGTTACTCTTCATCTTT+TGG - chr1.3:52809060-52809079 MS.gene055331:intron 35.0%
!! AATGGTGTTGTGAGTAATCA+TGG - chr1.3:52808805-52808824 MS.gene055331:intron 35.0%
!! ATCGGTGTCAATGTTATGTT+AGG + chr1.3:52809230-52809249 None:intergenic 35.0%
!! GCTTTTTCTGTGATGGAAAA+TGG - chr1.3:52808787-52808806 MS.gene055331:intron 35.0%
AGATGAAGAGTAACCAGAAG+AGG + chr1.3:52809058-52809077 None:intergenic 40.0%
CAGATTCTCTTCAAGACTTG+TGG - chr1.3:52808718-52808737 MS.gene055331:intron 40.0%
GCTATGACAGGACAAAATAC+AGG - chr1.3:52809545-52809564 MS.gene055331:CDS 40.0%
GTTCTCTCAGACAAACTATG+TGG - chr1.3:52808613-52808632 MS.gene055331:intron 40.0%
TATCCTAATGGGGTTCCAAA+GGG - chr1.3:52809003-52809022 MS.gene055331:intron 40.0%
TGATGAAGAAACGTGATGGT+AGG - chr1.3:52809645-52809664 MS.gene055331:CDS 40.0%
TGGCTAAATGATGCTATGAC+AGG - chr1.3:52809533-52809552 MS.gene055331:CDS 40.0%
TTATCCTAATGGGGTTCCAA+AGG - chr1.3:52809002-52809021 MS.gene055331:intron 40.0%
! CATAGTTTGTCTGAGAGAAC+TGG + chr1.3:52808613-52808632 None:intergenic 40.0%
! CTTCAGATTGAAGGTGTGTT+CGG + chr1.3:52809352-52809371 None:intergenic 40.0%
! GGTTACTCTTCATCTTTTGG+CGG - chr1.3:52809063-52809082 MS.gene055331:intron 40.0%
! GTCAAGTTGTTGTGTTGTGT+AGG - chr1.3:52809294-52809313 MS.gene055331:CDS 40.0%
!! AGATACCTGTGATGCTGTTT+TGG + chr1.3:52808534-52808553 None:intergenic 40.0%
!! CTGGTTTTCCACAAGTTTCA+GGG - chr1.3:52808692-52808711 MS.gene055331:intron 40.0%
!! GATGTTGGTGATTCTGATTG+TGG - chr1.3:52808880-52808899 MS.gene055331:intron 40.0%
!!! AGACACTGCTTTTTCTGTGA+TGG - chr1.3:52808780-52808799 MS.gene055331:intron 40.0%
AAGCTGAACTCTTGCAGAGA+TGG + chr1.3:52808644-52808663 None:intergenic 45.0%
AATGGCCAAAACAGCATCAC+AGG - chr1.3:52808526-52808545 MS.gene055331:intron 45.0%
ACAACAAATGCTGCTGGAAG+AGG - chr1.3:52808742-52808761 MS.gene055331:intron 45.0%
ACAGGTATCTTCCAATGCAG+AGG - chr1.3:52808544-52808563 MS.gene055331:intron 45.0%
AGGTTGTTACATGCTGTACG+AGG - chr1.3:52808666-52808685 MS.gene055331:intron 45.0%
ATCTGCATCCCTGAAACTTG+TGG + chr1.3:52808703-52808722 None:intergenic 45.0%
CAAAGAGCTCAAGTCGAATG+TGG - chr1.3:52808925-52808944 MS.gene055331:CDS 45.0%
CAACAAATGCTGCTGGAAGA+GGG - chr1.3:52808743-52808762 MS.gene055331:intron 45.0%
CAACTTGACAGTGATGCATG+TGG + chr1.3:52809283-52809302 None:intergenic 45.0%
TCTGCAAGAGTTCAGCTTTC+AGG - chr1.3:52808646-52808665 MS.gene055331:intron 45.0%
TGTGGAACAACAAATGCTGC+TGG - chr1.3:52808736-52808755 MS.gene055331:intron 45.0%
TTGTGAGATCTCCTCTGCAT+TGG + chr1.3:52808558-52808577 None:intergenic 45.0%
! ATTAGGAGGTATAGCTGCTG+TGG - chr1.3:52809586-52809605 MS.gene055331:CDS 45.0%
! GGTAGGTGTTGTGTTGTACA+TGG - chr1.3:52809662-52809681 MS.gene055331:CDS 45.0%
! TATAGCTGCTGTGGCGTTTT+TGG - chr1.3:52809595-52809614 MS.gene055331:CDS 45.0%
!! TATGTGATGGGTCAGTGTGA+GGG - chr1.3:52808856-52808875 MS.gene055331:intron 45.0%
!!! GCTGGTTTTCCACAAGTTTC+AGG - chr1.3:52808691-52808710 MS.gene055331:intron 45.0%
AAGGGTGGTCACTCTTCATC+TGG - chr1.3:52809021-52809040 MS.gene055331:intron 50.0%
GATGAAGAGTGACCACCCTT+TGG + chr1.3:52809021-52809040 None:intergenic 50.0%
TACATGCTGTACGAGGTTGC+TGG - chr1.3:52808673-52808692 MS.gene055331:intron 50.0%
TGCATGTATCCGACATGCGT+TGG + chr1.3:52809329-52809348 None:intergenic 50.0%
TGTCTGACACCAACGCATGT+CGG - chr1.3:52809317-52809336 MS.gene055331:CDS 50.0%
!! GTATGTGATGGGTCAGTGTG+AGG - chr1.3:52808855-52808874 MS.gene055331:intron 50.0%
CCTAATGGGGTTCCAAAGGG+TGG - chr1.3:52809006-52809025 MS.gene055331:intron 55.0%
GGTCACTCTTCATCTGGTGG+TGG - chr1.3:52809027-52809046 MS.gene055331:intron 55.0%
GGTCAGTGTGAGGGTGATGT+TGG - chr1.3:52808865-52808884 MS.gene055331:intron 55.0%
GGTGGTCACTCTTCATCTGG+TGG - chr1.3:52809024-52809043 MS.gene055331:intron 55.0%
!! CCACCCTTTGGAACCCCATT+AGG + chr1.3:52809009-52809028 None:intergenic 55.0%
!! GGTGGTGGATCATCCTCTTC+TGG - chr1.3:52809042-52809061 MS.gene055331:intron 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 52808298 52809703 52808298 ID=MS.gene055331
chr1.3 mRNA 52808298 52809703 52808298 ID=MS.gene055331.t1;Parent=MS.gene055331
chr1.3 exon 52809188 52809703 52809188 ID=MS.gene055331.t1.exon1;Parent=MS.gene055331.t1
chr1.3 CDS 52809188 52809703 52809188 ID=cds.MS.gene055331.t1;Parent=MS.gene055331.t1
chr1.3 exon 52808917 52808977 52808917 ID=MS.gene055331.t1.exon2;Parent=MS.gene055331.t1
chr1.3 CDS 52808917 52808977 52808917 ID=cds.MS.gene055331.t1;Parent=MS.gene055331.t1
chr1.3 exon 52808298 52808446 52808298 ID=MS.gene055331.t1.exon3;Parent=MS.gene055331.t1
chr1.3 CDS 52808298 52808446 52808298 ID=cds.MS.gene055331.t1;Parent=MS.gene055331.t1
Gene Sequence

>MS.gene055331

ATGGGTTTTCCAAAAAAACCCCTTTTTCTTCTTTTGTTCTTCATTTTCTTCACAACCCATTTCTCTAAATCATCATCTTCTCCTGATTACACAACTTTGGTCTACAAAGGTTGTTCAAAAGAAACATTCACAGATCCAAATGGAGCATATTCACAATCTCTTTCAGCACTTTTTGGTTCCTTAGTTTCACAATCCACAAAAACAAGGTTCTTCAAAACAACAACTGGTAATGGCCAAAACAGCATCACAGGTATCTTCCAATGCAGAGGAGATCTCACAAACTCAGATTGCTACAGCTGTGTTAGCAAGCTACCAGTTCTCTCAGACAAACTATGTGGCAAAACCATCTCTGCAAGAGTTCAGCTTTCAGGTTGTTACATGCTGTACGAGGTTGCTGGTTTTCCACAAGTTTCAGGGATGCAGATTCTCTTCAAGACTTGTGGAACAACAAATGCTGCTGGAAGAGGGTTTGAAGAGAGAAGAGACACTGCTTTTTCTGTGATGGAAAATGGTGTTGTGAGTAATCATGGTTTTTATGTTACTACTTATCAAGCTTTGTATGTGATGGGTCAGTGTGAGGGTGATGTTGGTGATTCTGATTGTGGTGAGTGTGTTAAATCTGCTGTTCAAAGAGCTCAAGTCGAATGTGGAAGTTCTATTTCAGGACAAATTTATTTGCATAAGTGCTTTATTAGTTTTAGTTATTATCCTAATGGGGTTCCAAAGGGTGGTCACTCTTCATCTGGTGGTGGATCATCCTCTTCTGGTTACTCTTCATCTTTTGGCGGTAAGTACTAATACACCATCATTCAGAATCAATAGATAAGATTGGTTTCAATTCTGGGTCATTATAAAGTTTTCATTTTTTTTTTACTTTTATGTTGAATCTGGTTTTGTGTTTGTTCATTTGACATCAATGAAGCAAGACACCTAACATAACATTGACACCGATCATAATTTGAGAAAATTGAATCGAATGGAACCACATGCATCACTGTCAAGTTGTTGTGTTGTGTAGGTGTCTGACACCAACGCATGTCGGATACATGCACCGAACACACCTTCAATCTGAAGTGTTAAGGTTATATATAGTTTCACACAGAAGTAGCTCAATTTAGCAATGGCAATACAATTCCTTAATTGTTCATGTGTTATAATTTTAAAGCAATTGTGCTACTTTGTTAAATACCATTTACTAATATGTATGAAACTTTAATTAATCATTGAGTTGTGTGTGGCTAAATGATGCTATGACAGGACAAAATACAGGAAAGACTGTGGCAATAATATTAGGAGGTATAGCTGCTGTGGCGTTTTTGGTTATATGCTTGCTATTTGCAAGGAATTTGATGAAGAAACGTGATGGTAGGTGTTGTGTTGTACATGGTTTTACTATATGGAATTGA

Protein sequence

>MS.gene055331.t1

MGFPKKPLFLLLFFIFFTTHFSKSSSSPDYTTLVYKGCSKETFTDPNGAYSQSLSALFGSLVSQSTKTRFFKTTTGNGQNSITGIFQCRGDLTNSDCYSCVSKLPVLSDKLCGKTISARVQLSGCYMLYEVAGFPQVSGMQILFKTCGTTNAAGRGFEERRDTAFSVMENGVGGHSSSGGGSSSSGYSSSFGGQNTGKTVAIILGGIAAVAFLVICLLFARNLMKKRDGRCCVVHGFTIWN