AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene62196

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62196.t1 MTR_3g068035 97.143 315 4 3 1 312 1 313 0.0 600
MS.gene62196.t1 MTR_1g044155 64.681 235 80 1 78 309 124 358 3.36e-79 245
MS.gene62196.t1 MTR_5g091630 52.232 224 84 2 80 303 47 247 2.98e-69 216
MS.gene62196.t1 MTR_1g079810 50.373 268 119 3 1 261 1 261 1.66e-68 216
MS.gene62196.t1 MTR_1g073860 76.974 152 35 0 78 229 92 243 2.05e-68 216
MS.gene62196.t1 MTR_5g080580 47.734 331 143 7 1 306 1 326 6.20e-67 213
MS.gene62196.t1 MTR_8g036060 62.136 206 67 2 28 233 45 239 8.29e-66 209
MS.gene62196.t1 MTR_5g011520 61.780 191 61 1 56 234 64 254 5.81e-65 207
MS.gene62196.t1 MTR_7g080980 67.516 157 45 1 89 245 66 216 1.18e-61 197
MS.gene62196.t1 MTR_4g098450 66.480 179 58 1 57 233 51 229 1.67e-60 195
MS.gene62196.t1 MTR_3g100470 40.728 302 135 10 17 312 27 290 1.37e-57 187
MS.gene62196.t1 MTR_5g066020 49.171 181 82 4 89 259 60 240 7.84e-45 153
MS.gene62196.t1 MTR_5g089600 37.423 163 91 2 76 227 73 235 9.39e-30 113
MS.gene62196.t1 MTR_6g061670 43.966 116 62 1 107 219 130 245 7.17e-24 99.8
MS.gene62196.t1 MTR_6g061670 43.966 116 62 1 107 219 125 240 7.65e-24 99.4
MS.gene62196.t1 MTR_5g010330 36.416 173 103 4 80 246 104 275 9.14e-24 99.4
MS.gene62196.t1 MTR_5g089700 36.646 161 92 3 72 223 67 226 2.52e-23 96.3
MS.gene62196.t1 MTR_1g073870 46.053 152 35 4 78 229 50 154 3.54e-23 95.5
MS.gene62196.t1 MTR_4g057487 33.929 168 99 4 72 229 11 176 5.18e-23 95.5
MS.gene62196.t1 MTR_1g061530 42.982 114 61 2 110 219 144 257 1.19e-22 97.1
MS.gene62196.t1 MTR_1g079780 42.857 112 63 1 110 220 112 223 2.17e-22 95.9
MS.gene62196.t1 MTR_1g032910 32.530 166 96 5 76 227 97 260 1.95e-21 92.4
MS.gene62196.t1 MTR_1g053685 32.515 163 101 2 76 229 81 243 1.95e-21 92.4
MS.gene62196.t1 MTR_4g057525 32.927 164 100 3 76 229 32 195 3.56e-21 90.9
MS.gene62196.t1 MTR_5g089090 34.177 158 88 2 80 226 28 180 4.48e-21 89.7
MS.gene62196.t1 MTR_7g077120 38.514 148 85 2 78 219 125 272 7.44e-20 89.0
MS.gene62196.t1 MTR_8g098390 36.496 137 83 3 99 232 158 293 1.03e-18 85.9
MS.gene62196.t1 MTR_1g076710 43.966 116 61 2 107 219 134 248 1.13e-18 85.5
MS.gene62196.t1 MTR_8g036160 36.943 157 83 3 110 262 145 289 1.33e-18 85.5
MS.gene62196.t1 MTR_7g116320 42.982 114 63 2 107 219 143 255 3.21e-18 84.3
MS.gene62196.t1 MTR_4g098460 36.486 148 77 4 80 219 57 195 7.10e-18 82.4
MS.gene62196.t1 MTR_7g116320 42.063 126 67 3 107 227 143 267 1.40e-17 81.3
MS.gene62196.t1 MTR_7g074935 43.363 113 61 1 110 219 130 242 1.89e-17 82.0
MS.gene62196.t1 MTR_8g031100 41.096 146 80 3 80 219 125 270 2.58e-17 81.6
MS.gene62196.t1 MTR_5g011680 35.115 131 71 3 110 233 145 268 9.11e-17 79.3
MS.gene62196.t1 MTR_4g098460 36.667 150 79 4 80 219 57 200 2.18e-16 78.2
MS.gene62196.t1 MTR_4g055813 37.903 124 75 2 110 231 22 145 4.28e-16 77.4
MS.gene62196.t1 MTR_8g078930 35.714 126 71 3 111 227 5 129 5.43e-16 74.3
MS.gene62196.t1 MTR_4g069110 36.634 101 64 0 74 174 28 128 7.44e-16 74.3
MS.gene62196.t1 MTR_4g055803 39.655 116 64 2 110 219 128 243 2.69e-15 76.3
MS.gene62196.t1 MTR_5g011620 31.210 157 92 4 111 265 119 261 3.00e-15 76.6
MS.gene62196.t1 MTR_4g055833 41.176 119 58 3 110 219 125 240 4.15e-15 75.1
MS.gene62196.t1 MTR_4g101990 42.982 114 61 1 110 219 147 260 1.46e-14 73.6
MS.gene62196.t1 MTR_6g060670 40.541 111 64 2 110 220 180 288 1.80e-14 73.2
MS.gene62196.t1 MTR_5g011610 33.333 117 75 2 111 226 62 176 1.98e-14 73.2
MS.gene62196.t1 MTR_5g011610 36.559 93 59 0 106 198 211 303 1.13e-12 67.8
MS.gene62196.t1 MTR_1g071850 33.113 151 73 3 88 238 38 160 2.84e-13 67.8
MS.gene62196.t1 MTR_1g038960 33.333 144 88 4 81 220 125 264 1.48e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene62196.t1 AT3G55560 53.235 340 101 10 1 312 1 310 3.05e-81 249
MS.gene62196.t1 AT4G14465 62.613 222 66 3 80 300 67 272 8.96e-76 234
MS.gene62196.t1 AT1G76500 60.541 185 63 2 56 231 49 232 2.82e-69 218
MS.gene62196.t1 AT3G04570 53.112 241 97 3 1 229 1 237 2.87e-65 208
MS.gene62196.t1 AT2G42940 49.778 225 86 3 79 303 52 249 1.53e-64 204
MS.gene62196.t1 AT2G45430 56.170 235 87 6 80 306 89 315 1.01e-63 204
MS.gene62196.t1 AT3G60870 52.423 227 86 4 80 306 59 263 2.62e-63 201
MS.gene62196.t1 AT2G35270 65.584 154 53 0 80 233 78 231 1.07e-62 200
MS.gene62196.t1 AT4G22810 70.064 157 44 1 80 233 105 261 5.92e-59 192
MS.gene62196.t1 AT4G12050 69.677 155 46 1 80 233 118 272 1.93e-58 191
MS.gene62196.t1 AT4G35390 71.333 150 41 1 80 227 63 212 4.60e-56 184
MS.gene62196.t1 AT5G49700 51.010 198 74 3 80 254 56 253 5.49e-56 183
MS.gene62196.t1 AT4G17800 66.207 145 49 0 89 233 91 235 2.04e-55 182
MS.gene62196.t1 AT1G20900 58.730 189 69 1 50 229 56 244 2.62e-53 177
MS.gene62196.t1 AT1G14490 50.877 171 75 3 70 233 14 182 1.02e-42 147
MS.gene62196.t1 AT1G14490 52.500 160 67 3 81 233 6 163 2.71e-42 145
MS.gene62196.t1 AT2G33620 43.243 148 80 3 75 219 132 278 7.78e-25 102
MS.gene62196.t1 AT2G33620 43.243 148 80 3 75 219 132 278 7.78e-25 102
MS.gene62196.t1 AT2G33620 43.243 148 80 3 75 219 132 278 7.78e-25 102
MS.gene62196.t1 AT2G33620 43.243 148 80 3 75 219 132 278 7.78e-25 102
MS.gene62196.t1 AT4G12080 44.538 119 59 2 110 223 173 289 1.10e-24 102
MS.gene62196.t1 AT4G25320 45.133 113 59 1 110 219 169 281 1.66e-23 100
MS.gene62196.t1 AT4G17950 42.759 145 76 3 80 219 196 338 2.10e-23 100
MS.gene62196.t1 AT5G51590 44.348 115 57 2 110 219 181 293 3.92e-23 99.0
MS.gene62196.t1 AT2G45850 41.096 146 80 3 80 219 132 277 6.01e-23 97.8
MS.gene62196.t1 AT2G45850 41.096 146 80 3 80 219 132 277 6.01e-23 97.8
MS.gene62196.t1 AT5G62260 38.710 124 73 1 107 227 160 283 7.89e-23 96.3
MS.gene62196.t1 AT1G63470 37.415 147 85 3 80 220 147 292 1.82e-22 96.7
MS.gene62196.t1 AT3G04590 42.727 110 63 0 110 219 174 283 3.12e-22 95.1
MS.gene62196.t1 AT3G04590 42.727 110 63 0 110 219 174 283 3.68e-22 96.3
MS.gene62196.t1 AT5G62260 39.655 116 67 1 107 219 160 275 1.02e-21 94.7
MS.gene62196.t1 AT5G62260 39.655 116 67 1 107 219 160 275 1.02e-21 94.7
MS.gene62196.t1 AT2G45850 40.136 147 82 3 80 220 132 278 3.05e-21 91.7
MS.gene62196.t1 AT4G00200 43.103 116 63 1 110 222 126 241 1.01e-17 82.4
MS.gene62196.t1 AT4G00200 43.103 116 63 1 110 222 126 241 1.01e-17 82.4
MS.gene62196.t1 AT4G22770 44.444 117 62 1 110 223 153 269 1.71e-17 82.0
MS.gene62196.t1 AT4G22770 44.444 117 62 1 110 223 153 269 1.71e-17 82.0
MS.gene62196.t1 AT5G46640 40.972 144 80 2 80 220 155 296 1.70e-16 79.7
MS.gene62196.t1 AT1G63480 32.051 156 94 4 70 219 125 274 1.99e-16 79.3
MS.gene62196.t1 AT1G63480 32.051 156 94 4 70 219 125 274 1.99e-16 79.3
MS.gene62196.t1 AT1G63480 32.051 156 94 4 70 219 119 268 2.18e-16 79.0
MS.gene62196.t1 AT1G63480 32.051 156 94 4 70 219 119 268 2.18e-16 79.0
MS.gene62196.t1 AT1G63480 32.051 156 94 4 70 219 119 268 2.18e-16 79.0
MS.gene62196.t1 AT1G63480 32.051 156 94 4 70 219 119 268 2.18e-16 79.0
MS.gene62196.t1 AT1G63480 32.051 156 94 4 70 219 119 268 2.18e-16 79.0
MS.gene62196.t1 AT3G61310 36.986 146 86 2 80 219 134 279 2.18e-16 79.0

Find 101 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCGAGATACTTTCGCTTTC+GGG 0.112808 3.2:-62732729 MS.gene62196:CDS
GCAGGTCCTTTGGTTGCTTC+TGG 0.205503 3.2:-62732615 MS.gene62196:CDS
GTACCATTATGCAACAAATT+TGG 0.220914 3.2:+62732366 None:intergenic
CAGGAGCAGCCGGCTGTCTA+AGG 0.232707 3.2:+62732778 None:intergenic
GTTGTTGGTGGTTCTGTTGC+AGG 0.265821 3.2:-62732633 MS.gene62196:CDS
CCTTCTCCGGCTGGAGCTAC+TGG 0.276244 3.2:-62732690 MS.gene62196:CDS
TTCTTCTCAAGGTTTGGAAT+TGG 0.278560 3.2:-62732442 MS.gene62196:CDS
GGTTATAACAATGGGTGGTT+TGG 0.288461 3.2:+62732944 None:intergenic
TTGCTGTTGCTGCTGTTTGT+TGG 0.296688 3.2:+62733080 None:intergenic
GAAGGAGGAGGACGAGGAGG+TGG 0.303163 3.2:+62732312 None:intergenic
GTTATAACAATGGGTGGTTT+GGG 0.305692 3.2:+62732945 None:intergenic
AAATGCCTCATGATGTGTTT+TGG 0.306421 3.2:-62732341 MS.gene62196:CDS
CCTCCAAATTTGTTGCATAA+TGG 0.306945 3.2:-62732369 MS.gene62196:CDS
TCTTCTCAAGGTTTGGAATT+GGG 0.309314 3.2:-62732441 MS.gene62196:CDS
GGTTGCTTCTGGACCTGTTA+TGG 0.315696 3.2:-62732604 MS.gene62196:CDS
TGTTTATTTAGCTGGTGGTC+AGG 0.322450 3.2:-62732661 MS.gene62196:CDS
GGTGGTCAGGGGCAGGTTGT+TGG 0.325929 3.2:-62732648 MS.gene62196:CDS
GGTGGTGTTATTACTCTTCA+TGG 0.329783 3.2:-62732756 MS.gene62196:CDS
TAGCTCTTCTTCTCAAGGTT+TGG 0.337613 3.2:-62732448 MS.gene62196:CDS
ATTCGAGATACTTTCGCTTT+CGG 0.341376 3.2:-62732730 MS.gene62196:CDS
CTACCTCCGCCTTCTCCGGC+TGG 0.357947 3.2:-62732699 MS.gene62196:CDS
ACATGGCTTCTTAGCGCGTT+GGG 0.359556 3.2:+62732912 None:intergenic
GAAAAGAGAGCAAGAGTTGA+TGG 0.369566 3.2:-62733138 MS.gene62196:CDS
GGATTAACTGTTTATTTAGC+TGG 0.378249 3.2:-62732669 MS.gene62196:CDS
GTAGCAGCAATAACCATAAC+AGG 0.415453 3.2:+62732591 None:intergenic
GTGGGCTGGAAATGTGGCTA+TGG 0.419505 3.2:-62733207 MS.gene62196:CDS
GGAGGAGGACGAGGAGGTGG+AGG 0.420358 3.2:+62732315 None:intergenic
TTTAGCTGGTGGTCAGGGGC+AGG 0.423340 3.2:-62732655 MS.gene62196:CDS
CTTAGACAGCCGGCTGCTCC+TGG 0.434867 3.2:-62732777 MS.gene62196:CDS
TGGTTCTGTTGCAGGTCCTT+TGG 0.443762 3.2:-62732625 MS.gene62196:CDS
GGTCAGGGGCAGGTTGTTGG+TGG 0.444022 3.2:-62732645 MS.gene62196:CDS
CCACTGCTACCTGGCTCAGA+TGG 0.446968 3.2:+62733011 None:intergenic
CTCCGACCACCACTGCTACC+TGG 0.451310 3.2:+62733002 None:intergenic
CGCGCTAAGAAGCCATGTCT+TGG 0.453833 3.2:-62732907 MS.gene62196:CDS
ATGGCAAATCGTTGGTGGGC+TGG 0.456372 3.2:-62733221 MS.gene62196:CDS
GACATGGCTTCTTAGCGCGT+TGG 0.461821 3.2:+62732911 None:intergenic
AGCTAACTCTTTCAGAAAGA+AGG 0.464345 3.2:+62732294 None:intergenic
GTTACTAGCTCTTCTTCTCA+AGG 0.469725 3.2:-62732453 MS.gene62196:CDS
GTTTATTTAGCTGGTGGTCA+GGG 0.471442 3.2:-62732660 MS.gene62196:CDS
CACTGCTACCTGGCTCAGAT+GGG 0.484019 3.2:+62733012 None:intergenic
GTGACGGCGGTTAGCAAAGT+TGG 0.484913 3.2:+62732848 None:intergenic
CAGAAAGAAGGAGGAGGACG+AGG 0.494395 3.2:+62732306 None:intergenic
GCATCATCAACAATTACAAC+AGG 0.495081 3.2:-62732415 MS.gene62196:CDS
ACCACCTTGTTCATCTTCCA+AGG 0.497250 3.2:+62732539 None:intergenic
TTTGGAGGCAAATTATACAT+AGG 0.498673 3.2:+62732384 None:intergenic
GAAATGCAAGTTCAACAATC+TGG 0.502411 3.2:-62732504 MS.gene62196:CDS
TTCAGATGTTCAAGTGATGA+TGG 0.505534 3.2:+62733158 None:intergenic
GATTCTTTGGTTATAACAAT+GGG 0.518538 3.2:+62732936 None:intergenic
GGATTCTTTGGTTATAACAA+TGG 0.520979 3.2:+62732935 None:intergenic
TACTTATGAGAGGTTGCCCT+TGG 0.521945 3.2:-62732556 MS.gene62196:CDS
TGGGCTGGAAATGTGGCTAT+GGG 0.522347 3.2:-62733206 MS.gene62196:CDS
CCGCCGTCACCGTGGTGTGT+CGG 0.526432 3.2:-62732835 MS.gene62196:CDS
GTAGCTCCAGCCGGAGAAGG+CGG 0.528688 3.2:+62732693 None:intergenic
CCAGTAGCTCCAGCCGGAGA+AGG 0.532784 3.2:+62732690 None:intergenic
CACTGCTGATTTCCAAGACA+TGG 0.534335 3.2:+62732895 None:intergenic
GAACAAAACCCATCTGAGCC+AGG 0.536402 3.2:-62733020 MS.gene62196:CDS
AGGCCACGTGGGAGACCACC+AGG 0.537133 3.2:-62732981 MS.gene62196:CDS
GCTCCAGCCGGAGAAGGCGG+AGG 0.543492 3.2:+62732696 None:intergenic
TTAACTGTTTATTTAGCTGG+TGG 0.548179 3.2:-62732666 MS.gene62196:CDS
AAACCGACACACCACGGTGA+CGG 0.553487 3.2:+62732832 None:intergenic
CCATTATGCAACAAATTTGG+AGG 0.558327 3.2:+62732369 None:intergenic
TAATGTAACCCTTAGACAGC+CGG 0.563997 3.2:-62732787 MS.gene62196:CDS
AGATTATGGCAAATCGTTGG+TGG 0.564432 3.2:-62733226 None:intergenic
ATAACACCACCAGGAGCAGC+CGG 0.567921 3.2:+62732768 None:intergenic
TTTATTTAGCTGGTGGTCAG+GGG 0.570136 3.2:-62732659 MS.gene62196:CDS
AGGAGCAGCCGGCTGTCTAA+GGG 0.570747 3.2:+62732779 None:intergenic
CATGGCTTCTTAGCGCGTTG+GGG 0.572748 3.2:+62732913 None:intergenic
TCAAGTGATGATGGTGATGA+AGG 0.576683 3.2:+62733167 None:intergenic
AGAAGATTATGGCAAATCGT+TGG 0.586611 3.2:-62733229 None:intergenic
TTGCTAATGCTACTTATGAG+AGG 0.586891 3.2:-62732566 MS.gene62196:CDS
TCTTTGGTTATAACAATGGG+TGG 0.587176 3.2:+62732939 None:intergenic
CTCTTTCAGAAAGAAGGAGG+AGG 0.588398 3.2:+62732300 None:intergenic
AGCCAGGTAGCAGTGGTGGT+CGG 0.589179 3.2:-62733004 MS.gene62196:CDS
AGTGGTGGTCGGAGGCCACG+TGG 0.591936 3.2:-62732993 MS.gene62196:CDS
GCAAGTTCAACAATCTGGTG+TGG 0.593311 3.2:-62732499 MS.gene62196:CDS
ATGGCTTCTTAGCGCGTTGG+GGG 0.597936 3.2:+62732914 None:intergenic
GATTATGGCAAATCGTTGGT+GGG 0.599720 3.2:-62733225 None:intergenic
TCTGAGCCAGGTAGCAGTGG+TGG 0.601683 3.2:-62733008 MS.gene62196:CDS
CCATCTGAGCCAGGTAGCAG+TGG 0.602057 3.2:-62733011 MS.gene62196:CDS
AGACAGCCGGCTGCTCCTGG+TGG 0.608408 3.2:-62732774 MS.gene62196:CDS
AAAGAAGGAGGAGGACGAGG+AGG 0.613275 3.2:+62732309 None:intergenic
CCGACACACCACGGTGACGG+CGG 0.613394 3.2:+62732835 None:intergenic
CAGGTAGCAGTGGTGGTCGG+AGG 0.616953 3.2:-62733001 MS.gene62196:CDS
GTTAATCCAGTAGCTCCAGC+CGG 0.618525 3.2:+62732684 None:intergenic
ACAAGGTGGTGGTGATCAAG+AGG 0.619032 3.2:-62732526 MS.gene62196:CDS
CCACCTTGTTCATCTTCCAA+GGG 0.619149 3.2:+62732540 None:intergenic
GTGGTGGTCGGAGGCCACGT+GGG 0.622577 3.2:-62732992 MS.gene62196:CDS
TCGTTGGTGGGCTGGAAATG+TGG 0.623949 3.2:-62733213 MS.gene62196:CDS
CAGCAGTGGTAGTGATGTGG+CGG 0.628263 3.2:-62732880 MS.gene62196:CDS
CACTCAAAACCGACACACCA+CGG 0.631096 3.2:+62732826 None:intergenic
TAACTCTTTCAGAAAGAAGG+AGG 0.635542 3.2:+62732297 None:intergenic
TTTGCTAACCGCCGTCACCG+TGG 0.638394 3.2:-62732843 MS.gene62196:CDS
GGACCCCAAAACACATCATG+AGG 0.638475 3.2:+62732336 None:intergenic
TTGGAAGATGAACAAGGTGG+TGG 0.639490 3.2:-62732537 MS.gene62196:CDS
GATCCTGGTGGTCTCCCACG+TGG 0.640272 3.2:+62732978 None:intergenic
TTGCCCTTGGAAGATGAACA+AGG 0.667444 3.2:-62732543 MS.gene62196:CDS
TGAAGAGTAATAACACCACC+AGG 0.674054 3.2:+62732759 None:intergenic
CATGTCTTGGAAATCAGCAG+TGG 0.685401 3.2:-62732894 MS.gene62196:CDS
ACAGGTCCAGAAGCAACCAA+AGG 0.692630 3.2:+62732609 None:intergenic
AATCAGCAGTGGTAGTGATG+TGG 0.709338 3.2:-62732883 MS.gene62196:CDS
CCCTTGGAAGATGAACAAGG+TGG 0.848232 3.2:-62732540 MS.gene62196:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! GATTCTTTGGTTATAACAAT+GGG + chr3.2:62732593-62732612 None:intergenic 25.0%
GGATTAACTGTTTATTTAGC+TGG - chr3.2:62732857-62732876 MS.gene62196:CDS 30.0%
GGATTCTTTGGTTATAACAA+TGG + chr3.2:62732594-62732613 None:intergenic 30.0%
GTACCATTATGCAACAAATT+TGG + chr3.2:62733163-62733182 None:intergenic 30.0%
TTAACTGTTTATTTAGCTGG+TGG - chr3.2:62732860-62732879 MS.gene62196:CDS 30.0%
TTTGGAGGCAAATTATACAT+AGG + chr3.2:62733145-62733164 None:intergenic 30.0%
ATTCGAGATACTTTCGCTTT+CGG - chr3.2:62732796-62732815 MS.gene62196:CDS 35.0%
CCATTATGCAACAAATTTGG+AGG + chr3.2:62733160-62733179 None:intergenic 35.0%
GAAATGCAAGTTCAACAATC+TGG - chr3.2:62733022-62733041 MS.gene62196:CDS 35.0%
GCATCATCAACAATTACAAC+AGG - chr3.2:62733111-62733130 MS.gene62196:CDS 35.0%
GTTATAACAATGGGTGGTTT+GGG + chr3.2:62732584-62732603 None:intergenic 35.0%
TCTTTGGTTATAACAATGGG+TGG + chr3.2:62732590-62732609 None:intergenic 35.0%
TTCAGATGTTCAAGTGATGA+TGG + chr3.2:62732371-62732390 None:intergenic 35.0%
! AAATGCCTCATGATGTGTTT+TGG - chr3.2:62733185-62733204 MS.gene62196:CDS 35.0%
! AATGCCTCATGATGTGTTTT+GGG - chr3.2:62733186-62733205 MS.gene62196:CDS 35.0%
! TCTTCTCAAGGTTTGGAATT+GGG - chr3.2:62733085-62733104 MS.gene62196:CDS 35.0%
! TTCTTCTCAAGGTTTGGAAT+TGG - chr3.2:62733084-62733103 MS.gene62196:CDS 35.0%
! TTGCTAATGCTACTTATGAG+AGG - chr3.2:62732960-62732979 MS.gene62196:CDS 35.0%
!! CCTCCAAATTTGTTGCATAA+TGG - chr3.2:62733157-62733176 MS.gene62196:CDS 35.0%
CAACAACAACAACAACGAAG+AGG - chr3.2:62732466-62732485 MS.gene62196:CDS 40.0%
GAAAAGAGAGCAAGAGTTGA+TGG - chr3.2:62732388-62732407 MS.gene62196:CDS 40.0%
GGTTATAACAATGGGTGGTT+TGG + chr3.2:62732585-62732604 None:intergenic 40.0%
GTAGCAGCAATAACCATAAC+AGG + chr3.2:62732938-62732957 None:intergenic 40.0%
GTTTATTTAGCTGGTGGTCA+GGG - chr3.2:62732866-62732885 MS.gene62196:CDS 40.0%
TAATGTAACCCTTAGACAGC+CGG - chr3.2:62732739-62732758 MS.gene62196:CDS 40.0%
TAGCTCTTCTTCTCAAGGTT+TGG - chr3.2:62733078-62733097 MS.gene62196:CDS 40.0%
TGAAGAGTAATAACACCACC+AGG + chr3.2:62732770-62732789 None:intergenic 40.0%
TGTTTATTTAGCTGGTGGTC+AGG - chr3.2:62732865-62732884 MS.gene62196:CDS 40.0%
TTCGAGATACTTTCGCTTTC+GGG - chr3.2:62732797-62732816 MS.gene62196:CDS 40.0%
TTTATTTAGCTGGTGGTCAG+GGG - chr3.2:62732867-62732886 MS.gene62196:CDS 40.0%
! ATGCCTCATGATGTGTTTTG+GGG - chr3.2:62733187-62733206 MS.gene62196:CDS 40.0%
! GTTACTAGCTCTTCTTCTCA+AGG - chr3.2:62733073-62733092 MS.gene62196:CDS 40.0%
!! GGTGGTGTTATTACTCTTCA+TGG - chr3.2:62732770-62732789 MS.gene62196:CDS 40.0%
!! TCAAGTGATGATGGTGATGA+AGG + chr3.2:62732362-62732381 None:intergenic 40.0%
!!! TTGGGTTTGTTTTTGGATCC+TGG + chr3.2:62732566-62732585 None:intergenic 40.0%
ACCACCTTGTTCATCTTCCA+AGG + chr3.2:62732990-62733009 None:intergenic 45.0%
CATGTCTTGGAAATCAGCAG+TGG - chr3.2:62732632-62732651 MS.gene62196:CDS 45.0%
CCACCTTGTTCATCTTCCAA+GGG + chr3.2:62732989-62733008 None:intergenic 45.0%
GCAAGTTCAACAATCTGGTG+TGG - chr3.2:62733027-62733046 MS.gene62196:CDS 45.0%
TACTTATGAGAGGTTGCCCT+TGG - chr3.2:62732970-62732989 MS.gene62196:CDS 45.0%
TTGCCCTTGGAAGATGAACA+AGG - chr3.2:62732983-62733002 MS.gene62196:CDS 45.0%
TTGGAAGATGAACAAGGTGG+TGG - chr3.2:62732989-62733008 MS.gene62196:CDS 45.0%
! AATCAGCAGTGGTAGTGATG+TGG - chr3.2:62732643-62732662 MS.gene62196:CDS 45.0%
! TTGCTGTTGCTGCTGTTTGT+TGG + chr3.2:62732449-62732468 None:intergenic 45.0%
!! CACTGCTGATTTCCAAGACA+TGG + chr3.2:62732634-62732653 None:intergenic 45.0%
!! GGGTGGTTTGGGTTTGTTTT+TGG + chr3.2:62732573-62732592 None:intergenic 45.0%
!!! GGTTTGTTTTTGGATCCTGG+TGG + chr3.2:62732563-62732582 None:intergenic 45.0%
ACAGGTCCAGAAGCAACCAA+AGG + chr3.2:62732920-62732939 None:intergenic 50.0%
ACATGGCTTCTTAGCGCGTT+GGG + chr3.2:62732617-62732636 None:intergenic 50.0%
CACTCAAAACCGACACACCA+CGG + chr3.2:62732703-62732722 None:intergenic 50.0%
CCCTTGGAAGATGAACAAGG+TGG - chr3.2:62732986-62733005 MS.gene62196:CDS 50.0%
GAACAAAACCCATCTGAGCC+AGG - chr3.2:62732506-62732525 MS.gene62196:CDS 50.0%
GGACCCCAAAACACATCATG+AGG + chr3.2:62733193-62733212 None:intergenic 50.0%
GGTTGCTTCTGGACCTGTTA+TGG - chr3.2:62732922-62732941 MS.gene62196:CDS 50.0%
GTTAATCCAGTAGCTCCAGC+CGG + chr3.2:62732845-62732864 None:intergenic 50.0%
! ACAAGGTGGTGGTGATCAAG+AGG - chr3.2:62733000-62733019 MS.gene62196:CDS 50.0%
! TGGGCTGGAAATGTGGCTAT+GGG - chr3.2:62732320-62732339 MS.gene62196:CDS 50.0%
! TGGTTCTGTTGCAGGTCCTT+TGG - chr3.2:62732901-62732920 MS.gene62196:CDS 50.0%
! TTTTCTACCTCCGCCTTCTC+CGG - chr3.2:62732823-62732842 MS.gene62196:CDS 50.0%
!! GTTGTTGGTGGTTCTGTTGC+AGG - chr3.2:62732893-62732912 MS.gene62196:CDS 50.0%
!!! GTGGTGTGTCGGTTTTGAGT+GGG - chr3.2:62732702-62732721 MS.gene62196:CDS 50.0%
AAACCGACACACCACGGTGA+CGG + chr3.2:62732697-62732716 None:intergenic 55.0%
AAAGAAGGAGGAGGACGAGG+AGG + chr3.2:62733220-62733239 None:intergenic 55.0%
ATAACACCACCAGGAGCAGC+CGG + chr3.2:62732761-62732780 None:intergenic 55.0%
ATGGCTTCTTAGCGCGTTGG+GGG + chr3.2:62732615-62732634 None:intergenic 55.0%
CACTGCTACCTGGCTCAGAT+GGG + chr3.2:62732517-62732536 None:intergenic 55.0%
CAGAAAGAAGGAGGAGGACG+AGG + chr3.2:62733223-62733242 None:intergenic 55.0%
CATGGCTTCTTAGCGCGTTG+GGG + chr3.2:62732616-62732635 None:intergenic 55.0%
CGCGCTAAGAAGCCATGTCT+TGG - chr3.2:62732619-62732638 MS.gene62196:CDS 55.0%
GACATGGCTTCTTAGCGCGT+TGG + chr3.2:62732618-62732637 None:intergenic 55.0%
GCAGGTCCTTTGGTTGCTTC+TGG - chr3.2:62732911-62732930 MS.gene62196:CDS 55.0%
GTGACGGCGGTTAGCAAAGT+TGG + chr3.2:62732681-62732700 None:intergenic 55.0%
TAGCGCGTTGGGGGATTCTT+TGG + chr3.2:62732606-62732625 None:intergenic 55.0%
! ATGGCAAATCGTTGGTGGGC+TGG - chr3.2:62732305-62732324 MS.gene62196:CDS 55.0%
! CAGCAGTGGTAGTGATGTGG+CGG - chr3.2:62732646-62732665 MS.gene62196:CDS 55.0%
!! GTGGGCTGGAAATGTGGCTA+TGG - chr3.2:62732319-62732338 MS.gene62196:CDS 55.0%
!! TCGTTGGTGGGCTGGAAATG+TGG - chr3.2:62732313-62732332 MS.gene62196:CDS 55.0%
!!! CGTGGTGTGTCGGTTTTGAG+TGG - chr3.2:62732701-62732720 MS.gene62196:CDS 55.0%
!!! GTGTCGGTTTTGAGTGGGAG+CGG - chr3.2:62732707-62732726 MS.gene62196:CDS 55.0%
AGCCAGGTAGCAGTGGTGGT+CGG - chr3.2:62732522-62732541 MS.gene62196:CDS 60.0%
AGGAGCAGCCGGCTGTCTAA+GGG + chr3.2:62732750-62732769 None:intergenic 60.0%
CCACTGCTACCTGGCTCAGA+TGG + chr3.2:62732518-62732537 None:intergenic 60.0%
CCATCTGAGCCAGGTAGCAG+TGG - chr3.2:62732515-62732534 MS.gene62196:CDS 60.0%
TCTGAGCCAGGTAGCAGTGG+TGG - chr3.2:62732518-62732537 MS.gene62196:CDS 60.0%
TTTAGCTGGTGGTCAGGGGC+AGG - chr3.2:62732871-62732890 MS.gene62196:CDS 60.0%
TTTGCTAACCGCCGTCACCG+TGG - chr3.2:62732683-62732702 MS.gene62196:CDS 60.0%
CAGGAGCAGCCGGCTGTCTA+AGG + chr3.2:62732751-62732770 None:intergenic 65.0%
CAGGTAGCAGTGGTGGTCGG+AGG - chr3.2:62732525-62732544 MS.gene62196:CDS 65.0%
CCAGTAGCTCCAGCCGGAGA+AGG + chr3.2:62732839-62732858 None:intergenic 65.0%
CCTTCTCCGGCTGGAGCTAC+TGG - chr3.2:62732836-62732855 MS.gene62196:CDS 65.0%
CTCCGACCACCACTGCTACC+TGG + chr3.2:62732527-62732546 None:intergenic 65.0%
CTTAGACAGCCGGCTGCTCC+TGG - chr3.2:62732749-62732768 MS.gene62196:CDS 65.0%
GAAGGAGGAGGACGAGGAGG+TGG + chr3.2:62733217-62733236 None:intergenic 65.0%
GATCCTGGTGGTCTCCCACG+TGG + chr3.2:62732551-62732570 None:intergenic 65.0%
GGTCAGGGGCAGGTTGTTGG+TGG - chr3.2:62732881-62732900 MS.gene62196:CDS 65.0%
GGTGGTCAGGGGCAGGTTGT+TGG - chr3.2:62732878-62732897 MS.gene62196:CDS 65.0%
GTAGCTCCAGCCGGAGAAGG+CGG + chr3.2:62732836-62732855 None:intergenic 65.0%
AGACAGCCGGCTGCTCCTGG+TGG - chr3.2:62732752-62732771 MS.gene62196:CDS 70.0%
AGGCCACGTGGGAGACCACC+AGG - chr3.2:62732545-62732564 MS.gene62196:CDS 70.0%
AGTGGTGGTCGGAGGCCACG+TGG - chr3.2:62732533-62732552 MS.gene62196:CDS 70.0%
CCGCCGTCACCGTGGTGTGT+CGG - chr3.2:62732691-62732710 MS.gene62196:CDS 70.0%
CTACCTCCGCCTTCTCCGGC+TGG - chr3.2:62732827-62732846 MS.gene62196:CDS 70.0%
GGAGGAGGACGAGGAGGTGG+AGG + chr3.2:62733214-62733233 None:intergenic 70.0%
GTGGTGGTCGGAGGCCACGT+GGG - chr3.2:62732534-62732553 MS.gene62196:CDS 70.0%
! CCGACACACCACGGTGACGG+CGG + chr3.2:62732694-62732713 None:intergenic 70.0%
GCTCCAGCCGGAGAAGGCGG+AGG + chr3.2:62732833-62732852 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 62732305 62733243 62732305 ID=MS.gene62196
chr3.2 mRNA 62732305 62733243 62732305 ID=MS.gene62196.t1;Parent=MS.gene62196
chr3.2 exon 62732305 62733243 62732305 ID=MS.gene62196.t1.exon1;Parent=MS.gene62196.t1
chr3.2 CDS 62732305 62733243 62732305 ID=cds.MS.gene62196.t1;Parent=MS.gene62196.t1
Gene Sequence

>MS.gene62196

ATGGCAAATCGTTGGTGGGCTGGAAATGTGGCTATGGGTCACAGAGAATCAATGCCTTCATCACCATCATCACTTGAACATCTGAAAAGAGAGCAAGAGTTGATGGAAAACAACAACAACAACAACAACGCGAACTCAACTCCAACAAACAGCAGCAACAGCAACAACAACAACAACGAAGAGGAAGACAACAACAACGAAGAACAAAACCCATCTGAGCCAGGTAGCAGTGGTGGTCGGAGGCCACGTGGGAGACCACCAGGATCCAAAAACAAACCCAAACCACCCATTGTTATAACCAAAGAATCCCCCAACGCGCTAAGAAGCCATGTCTTGGAAATCAGCAGTGGTAGTGATGTGGCGGAGAGTATTGCCAACTTTGCTAACCGCCGTCACCGTGGTGTGTCGGTTTTGAGTGGGAGCGGCATTGTAGCTAATGTAACCCTTAGACAGCCGGCTGCTCCTGGTGGTGTTATTACTCTTCATGGAAGATTCGAGATACTTTCGCTTTCGGGTGCTTTTCTACCTCCGCCTTCTCCGGCTGGAGCTACTGGATTAACTGTTTATTTAGCTGGTGGTCAGGGGCAGGTTGTTGGTGGTTCTGTTGCAGGTCCTTTGGTTGCTTCTGGACCTGTTATGGTTATTGCTGCTACTTTTGCTAATGCTACTTATGAGAGGTTGCCCTTGGAAGATGAACAAGGTGGTGGTGATCAAGAGGAAATGCAAGTTCAACAATCTGGTGTGGATTCAGCAACTGTTGCAGCTGCAGTTACTAGCTCTTCTTCTCAAGGTTTGGAATTGGGTGAGCATCATCAACAATTACAACAGGTTTCAATGCCTATGTATAATTTGCCTCCAAATTTGTTGCATAATGGTACTCAAATGCCTCATGATGTGTTTTGGGGTCCTCCACCTCCTCGTCCTCCTCCTTCTTTCTGA
Protein sequence

>MS.gene62196.t1

MANRWWAGNVAMGHRESMPSSPSSLEHLKREQELMENNNNNNNANSTPTNSSNSNNNNNEEEDNNNEEQNPSEPGSSGGRRPRGRPPGSKNKPKPPIVITKESPNALRSHVLEISSGSDVAESIANFANRRHRGVSVLSGSGIVANVTLRQPAAPGGVITLHGRFEILSLSGAFLPPPSPAGATGLTVYLAGGQGQVVGGSVAGPLVASGPVMVIAATFANATYERLPLEDEQGGGDQEEMQVQQSGVDSATVAAAVTSSSSQGLELGEHHQQLQQVSMPMYNLPPNLLHNGTQMPHDVFWGPPPPRPPPSF