Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63928.t1 | AES68729.1 | 100 | 77 | 0 | 0 | 1 | 77 | 1 | 77 | 9.00E-14 | 85.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63928.t1 | G7J0G8 | 100.0 | 77 | 0 | 0 | 1 | 77 | 1 | 77 | 6.5e-14 | 85.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050381 | MS.gene63928 | 0.801793 | 7.62E-49 | -1.69E-46 |
| MS.gene050479 | MS.gene63928 | 0.805045 | 1.61E-49 | -1.69E-46 |
| MS.gene050547 | MS.gene63928 | 0.80562 | 1.22E-49 | -1.69E-46 |
| MS.gene050937 | MS.gene63928 | 0.805676 | 1.19E-49 | -1.69E-46 |
| MS.gene051946 | MS.gene63928 | 0.807599 | 4.67E-50 | -1.69E-46 |
| MS.gene051973 | MS.gene63928 | 0.811752 | 5.98E-51 | -1.69E-46 |
| MS.gene052015 | MS.gene63928 | 0.829543 | 4.85E-55 | -1.69E-46 |
| MS.gene05234 | MS.gene63928 | 0.830012 | 3.73E-55 | -1.69E-46 |
| MS.gene052825 | MS.gene63928 | -0.800617 | 1.33E-48 | -1.69E-46 |
| MS.gene056988 | MS.gene63928 | 0.81735 | 3.45E-52 | -1.69E-46 |
| MS.gene058157 | MS.gene63928 | -0.802438 | 5.61E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63928.t1 | MTR_3g014180 | 98.701 | 77 | 1 | 0 | 1 | 77 | 1 | 77 | 1.20e-45 | 140 |
| MS.gene63928.t1 | MTR_1g037690 | 97.403 | 77 | 1 | 1 | 1 | 77 | 1 | 76 | 3.51e-43 | 134 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGTTTATTCTTCTTCTTCTT+CGG | 0.278960 | 3.4:+10175819 | MS.gene63928:CDS |
| CATCGCACTTGCAGCGTCTA+TGG | 0.298343 | 3.4:+10175669 | MS.gene63928:CDS |
| TTTCTTCCCCGTCGGAATCA+TGG | 0.349115 | 3.4:+10175744 | MS.gene63928:CDS |
| ATGGTTCGTTGTTTCAGCTT+AGG | 0.415115 | 3.4:+10175631 | MS.gene63928:CDS |
| TGTTCTCGCTTTCTCGCCTT+CGG | 0.415823 | 3.4:+10175777 | MS.gene63928:CDS |
| AAAAGAAGAGCCAACGGCGG+TGG | 0.459419 | 3.4:-10175724 | None:intergenic |
| GCCAACGGCGGTGGAGGTAG+CGG | 0.473788 | 3.4:-10175715 | None:intergenic |
| AGAAGAGCCAACGGCGGTGG+AGG | 0.503798 | 3.4:-10175721 | None:intergenic |
| GCCGCTACCTCCACCGCCGT+TGG | 0.524262 | 3.4:+10175714 | MS.gene63928:CDS |
| AGCGCCGCCATGATTCCGAC+GGG | 0.539435 | 3.4:-10175751 | None:intergenic |
| CGGGGATCGCGAATTCGAAA+CGG | 0.579706 | 3.4:+10175839 | MS.gene63928:CDS |
| AAGTGCGATGAGGATCAAAA+CGG | 0.585253 | 3.4:-10175656 | None:intergenic |
| GGTAGCGGCGGAAGTATCAA+AGG | 0.588877 | 3.4:-10175700 | None:intergenic |
| AAGCGCCGCCATGATTCCGA+CGG | 0.596904 | 3.4:-10175752 | None:intergenic |
| AACGGCGGTGGAGGTAGCGG+CGG | 0.615025 | 3.4:-10175712 | None:intergenic |
| GGGAAGAAAAGAAGAGCCAA+CGG | 0.632187 | 3.4:-10175730 | None:intergenic |
| CTTCCCCGTCGGAATCATGG+CGG | 0.655059 | 3.4:+10175747 | MS.gene63928:CDS |
| TAGACGCTGCAAGTGCGATG+AGG | 0.674020 | 3.4:-10175666 | None:intergenic |
| AAGAAAAGAAGAGCCAACGG+CGG | 0.744971 | 3.4:-10175727 | None:intergenic |
| GCGCCGCCATGATTCCGACG+GGG | 0.755896 | 3.4:-10175750 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| CGTTTATTCTTCTTCTTCTT+CGG | + | chr3.4:10175819-10175838 | MS.gene63928:CDS | 30.0% | |
| GTTTATTCTTCTTCTTCTTC+GGG | + | chr3.4:10175820-10175839 | MS.gene63928:CDS | 30.0% | |
| TTTATTCTTCTTCTTCTTCG+GGG | + | chr3.4:10175821-10175840 | MS.gene63928:CDS | 30.0% | |
| ! | ACGTTTTTAACATTCTCCGA+AGG | - | chr3.4:10175796-10175815 | None:intergenic | 35.0% |
| AAGTGCGATGAGGATCAAAA+CGG | - | chr3.4:10175659-10175678 | None:intergenic | 40.0% | |
| ! | ATGGTTCGTTGTTTCAGCTT+AGG | + | chr3.4:10175631-10175650 | MS.gene63928:CDS | 40.0% |
| AAGAAAAGAAGAGCCAACGG+CGG | - | chr3.4:10175730-10175749 | None:intergenic | 45.0% | |
| GGGAAGAAAAGAAGAGCCAA+CGG | - | chr3.4:10175733-10175752 | None:intergenic | 45.0% | |
| TGTTCTCGCTTTCTCGCCTT+CGG | + | chr3.4:10175777-10175796 | MS.gene63928:CDS | 50.0% | |
| TTTCTTCCCCGTCGGAATCA+TGG | + | chr3.4:10175744-10175763 | MS.gene63928:CDS | 50.0% | |
| ! | GCTCTTCTTTTCTTCCCCGT+CGG | + | chr3.4:10175736-10175755 | MS.gene63928:CDS | 50.0% |
| AAAAGAAGAGCCAACGGCGG+TGG | - | chr3.4:10175727-10175746 | None:intergenic | 55.0% | |
| CATCGCACTTGCAGCGTCTA+TGG | + | chr3.4:10175669-10175688 | MS.gene63928:CDS | 55.0% | |
| CGGGGATCGCGAATTCGAAA+CGG | + | chr3.4:10175839-10175858 | MS.gene63928:CDS | 55.0% | |
| TAGACGCTGCAAGTGCGATG+AGG | - | chr3.4:10175669-10175688 | None:intergenic | 55.0% | |
| !! | GGTAGCGGCGGAAGTATCAA+AGG | - | chr3.4:10175703-10175722 | None:intergenic | 55.0% |
| CTTCCCCGTCGGAATCATGG+CGG | + | chr3.4:10175747-10175766 | MS.gene63928:CDS | 60.0% | |
| ! | AAGCGCCGCCATGATTCCGA+CGG | - | chr3.4:10175755-10175774 | None:intergenic | 60.0% |
| AGAAGAGCCAACGGCGGTGG+AGG | - | chr3.4:10175724-10175743 | None:intergenic | 65.0% | |
| AGCGCCGCCATGATTCCGAC+GGG | - | chr3.4:10175754-10175773 | None:intergenic | 65.0% | |
| GCCAACGGCGGTGGAGGTAG+CGG | - | chr3.4:10175718-10175737 | None:intergenic | 70.0% | |
| GCGCCGCCATGATTCCGACG+GGG | - | chr3.4:10175753-10175772 | None:intergenic | 70.0% | |
| ! | AACGGCGGTGGAGGTAGCGG+CGG | - | chr3.4:10175715-10175734 | None:intergenic | 70.0% |
| GCCGCTACCTCCACCGCCGT+TGG | + | chr3.4:10175714-10175733 | MS.gene63928:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 10175631 | 10175864 | 10175631 | ID=MS.gene63928 |
| chr3.4 | mRNA | 10175631 | 10175864 | 10175631 | ID=MS.gene63928.t1;Parent=MS.gene63928 |
| chr3.4 | exon | 10175631 | 10175864 | 10175631 | ID=MS.gene63928.t1.exon1;Parent=MS.gene63928.t1 |
| chr3.4 | CDS | 10175631 | 10175864 | 10175631 | ID=cds.MS.gene63928.t1;Parent=MS.gene63928.t1 |
| Gene Sequence |
| Protein sequence |
