AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene64394

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64394.t1 MTR_2g054940 99.217 511 4 0 1 511 1 511 0.0 1054
MS.gene64394.t1 MTR_7g081695 95.644 505 10 1 1 505 1 493 0.0 989
MS.gene64394.t1 MTR_4g113280 87.550 498 51 1 14 511 3 489 0.0 910
MS.gene64394.t1 MTR_1g076430 75.692 506 123 0 6 511 7 512 0.0 823
MS.gene64394.t1 MTR_1g041085 77.688 493 110 0 19 511 26 518 0.0 818
MS.gene64394.t1 MTR_4g070270 67.262 504 161 2 2 505 3 502 0.0 713
MS.gene64394.t1 MTR_7g118170 65.737 502 157 6 17 506 31 529 0.0 666
MS.gene64394.t1 MTR_1g110930 63.136 491 180 1 18 508 4 493 0.0 638
MS.gene64394.t1 MTR_3g105550 57.884 501 199 4 17 505 36 536 0.0 625
MS.gene64394.t1 MTR_5g056510 60.081 491 195 1 18 508 4 493 0.0 617
MS.gene64394.t1 MTR_6g074880 60.699 458 174 2 56 507 13 470 0.0 576
MS.gene64394.t1 MTR_4g054630 59.477 459 179 3 53 505 9 466 0.0 565
MS.gene64394.t1 MTR_4g054415 62.919 418 154 1 89 505 8 425 0.0 560
MS.gene64394.t1 MTR_6g032965 61.818 385 147 0 122 506 1 385 0.0 513
MS.gene64394.t1 MTR_2g013740 53.734 482 209 5 19 497 33 503 1.76e-171 493
MS.gene64394.t1 MTR_7g118160 55.198 404 137 3 102 503 56 417 2.37e-159 459
MS.gene64394.t1 MTR_2g436480 49.890 455 210 7 63 505 3 451 3.17e-157 455
MS.gene64394.t1 MTR_2g007020 52.683 410 191 3 98 506 45 452 6.10e-157 454
MS.gene64394.t1 MTR_1g102290 47.059 408 215 1 97 503 77 484 3.26e-135 400
MS.gene64394.t1 MTR_3g072980 46.472 411 218 2 94 503 74 483 1.49e-130 388
MS.gene64394.t1 MTR_3g072940 46.472 411 218 2 94 503 74 483 2.06e-130 388
MS.gene64394.t1 MTR_6g051440 46.287 404 215 2 101 503 80 482 7.06e-120 361
MS.gene64394.t1 MTR_1g115560 39.954 433 227 7 98 504 28 453 8.87e-109 332
MS.gene64394.t1 MTR_4g062270 39.817 437 245 6 76 502 5 433 7.34e-107 327
MS.gene64394.t1 MTR_4g062190 38.170 448 250 5 72 502 5 442 4.85e-106 325
MS.gene64394.t1 MTR_2g096210 39.813 427 226 6 98 502 27 444 2.32e-104 321
MS.gene64394.t1 MTR_4g062170 38.514 444 253 5 72 502 5 441 2.88e-102 315
MS.gene64394.t1 MTR_4g054260 58.203 256 89 2 251 505 87 325 1.79e-99 303
MS.gene64394.t1 MTR_4g053905 67.296 159 52 0 347 505 1 159 1.21e-73 230
MS.gene64394.t1 MTR_4g054265 50.893 112 55 0 87 198 6 117 3.49e-34 125
MS.gene64394.t1 MTR_7g086260 59.615 52 21 0 345 396 25 76 1.43e-13 66.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene64394.t1 AT2G26640 82.515 509 88 1 3 511 2 509 0.0 898
MS.gene64394.t1 AT1G04220 71.881 505 133 5 15 511 18 521 0.0 762
MS.gene64394.t1 AT5G43760 74.037 493 122 3 25 511 34 526 0.0 734
MS.gene64394.t1 AT1G19440 68.712 489 152 1 15 503 25 512 0.0 714
MS.gene64394.t1 AT2G16280 67.894 489 156 1 17 505 23 510 0.0 704
MS.gene64394.t1 AT1G01120 62.828 495 178 3 17 505 32 526 0.0 659
MS.gene64394.t1 AT1G68530 61.837 490 186 1 19 508 6 494 0.0 643
MS.gene64394.t1 AT4G34510 63.312 477 168 3 30 505 15 485 0.0 637
MS.gene64394.t1 AT1G25450 61.224 490 189 1 19 508 1 489 0.0 628
MS.gene64394.t1 AT2G26250 55.048 525 203 5 8 503 28 548 0.0 599
MS.gene64394.t1 AT4G34250 57.851 484 197 3 28 505 5 487 0.0 572
MS.gene64394.t1 AT4G34520 57.819 486 193 3 31 505 6 490 0.0 571
MS.gene64394.t1 AT2G15090 58.511 470 184 5 45 505 12 479 0.0 557
MS.gene64394.t1 AT2G46720 60.142 424 164 2 87 505 42 465 0.0 552
MS.gene64394.t1 AT3G10280 58.962 424 162 3 87 505 42 458 0.0 533
MS.gene64394.t1 AT1G71160 50.943 424 204 4 82 503 25 446 3.47e-158 458
MS.gene64394.t1 AT1G68530 57.849 344 145 0 19 362 6 349 1.14e-145 423
MS.gene64394.t1 AT5G49070 49.412 425 207 4 87 506 35 456 1.47e-144 423
MS.gene64394.t1 AT3G52160 44.969 487 197 5 20 494 22 449 2.16e-141 415
MS.gene64394.t1 AT3G52160 50.959 365 148 2 130 494 3 336 6.22e-131 384
MS.gene64394.t1 AT2G28630 40.515 427 225 7 98 502 27 446 1.13e-106 327
MS.gene64394.t1 AT2G28630 40.515 427 225 7 98 502 27 446 1.13e-106 327
MS.gene64394.t1 AT5G04530 39.810 422 243 5 89 502 19 437 1.16e-106 326
MS.gene64394.t1 AT1G07720 40.758 422 232 4 98 502 27 447 1.79e-105 324
MS.gene64394.t1 AT1G07720 40.758 422 232 4 98 502 70 490 2.74e-105 325
MS.gene64394.t1 AT5G49070 44.444 279 154 1 87 364 35 313 5.93e-80 252
MS.gene64394.t1 AT4G34850 25.483 259 149 10 236 482 91 317 4.04e-12 67.8
MS.gene64394.t1 AT4G34850 25.483 259 149 10 236 482 164 390 7.79e-12 67.4

Find 135 sgRNAs with CRISPR-Local

Find 139 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAATGATATCTATGATATC+TGG 0.226248 2.4:-35369143 MS.gene64394:CDS
ATGCACTAAGAGGATATTTA+TGG 0.261715 2.4:-35368980 MS.gene64394:CDS
TACTTTATCAAAAGATCTTA+TGG 0.267099 2.4:-35368311 MS.gene64394:CDS
AGAATGACACTTTATCGTTT+TGG 0.268393 2.4:-35368043 MS.gene64394:CDS
GGTGGGATGGGATGCAGTGC+TGG 0.280642 2.4:-35368631 MS.gene64394:CDS
CGATGAGATCCACCAGTTTC+CGG 0.286351 2.4:-35367846 MS.gene64394:CDS
TTCCTAGCTTCTTTCATTGA+AGG 0.317480 2.4:+35368832 None:intergenic
ATTTAGATGGCAGAAATTCT+TGG 0.321018 2.4:+35367815 None:intergenic
ATCAGAATTCCAATGTCTTT+AGG 0.328553 2.4:+35368748 None:intergenic
AGCTTGAAATCCGGAATATA+AGG 0.330746 2.4:+35368163 None:intergenic
GGTGTGGACCAATTGGTATT+TGG 0.335798 2.4:+35368414 None:intergenic
TGGCAAATAGCATTTGGTTC+TGG 0.340846 2.4:-35367941 MS.gene64394:CDS
AAAACAAACATTACCACGTT+GGG 0.342115 2.4:-35368268 MS.gene64394:CDS
GTGCATTTGCGAGACTCTTC+CGG 0.354359 2.4:+35368997 None:intergenic
TCCTTTCTTGATCCTTCCCT+TGG 0.368172 2.4:+35367973 None:intergenic
CTGCAATTCTACTTTCCAAC+AGG 0.368912 2.4:-35368456 MS.gene64394:CDS
AAAGATCTTATGGCCGTTGC+AGG 0.375775 2.4:-35368301 MS.gene64394:CDS
TTCGAATTGTTTGTTCCGTA+TGG 0.380907 2.4:-35368485 MS.gene64394:CDS
AGCTTCTTTCATTGAAGGAT+TGG 0.386247 2.4:+35368837 None:intergenic
TTTAAACTACATCATTTAGA+TGG 0.399579 2.4:+35367802 None:intergenic
ACAACCAACGCATACGAGTT+AGG 0.410167 2.4:+35368565 None:intergenic
AAGCTTGGATCGATCGTTCC+CGG 0.410332 2.4:+35368510 None:intergenic
AGGACGAGTCATAAAGTAAA+AGG 0.410912 2.4:+35369056 None:intergenic
GCTTCTTTCATTGAAGGATT+GGG 0.411634 2.4:+35368838 None:intergenic
CTGTTCTTCGGTACGTTAGT+TGG 0.413011 2.4:-35368214 MS.gene64394:CDS
TTGGAACATCGACCGGAAAC+TGG 0.414672 2.4:+35367834 None:intergenic
GAAGATAAAGCCTTATATTC+CGG 0.417524 2.4:-35368173 MS.gene64394:CDS
GCGCAGTATGGAAGGCTCTA+AGG 0.417809 2.4:-35367904 MS.gene64394:CDS
AGAACATGGCAAATAGCATT+TGG 0.419756 2.4:-35367947 MS.gene64394:CDS
GAAGCGGAGGCAGTTATGTT+TGG 0.419896 2.4:-35368808 MS.gene64394:CDS
TCAAGTGCAACAGCGCAGTA+TGG 0.422431 2.4:-35367916 MS.gene64394:CDS
CGATAAAGTGTCATTCTTGA+CGG 0.427252 2.4:+35368049 None:intergenic
GGAATGCTTAGAACAGCTTC+TGG 0.428265 2.4:+35368862 None:intergenic
TGGAGAACATCACGTTGAAT+TGG 0.436866 2.4:-35368537 MS.gene64394:CDS
ATCCTTGAGAGATCTGGTCT+TGG 0.439851 2.4:-35368901 MS.gene64394:CDS
CGAAAGATCCTTGAGAGATC+TGG 0.441571 2.4:-35368907 MS.gene64394:CDS
TGCGAACGGTGTGGACCAAT+TGG 0.448066 2.4:+35368407 None:intergenic
GAAGCTGTCTCCATGGCATA+TGG 0.449190 2.4:-35368074 MS.gene64394:CDS
GGTACATGCCATGAGTGATT+AGG 0.451469 2.4:+35369220 None:intergenic
CAGTTCGCTTTGGTACGAAT+TGG 0.452777 2.4:-35368008 MS.gene64394:CDS
GTGGGATGGGATGCAGTGCT+GGG 0.466651 2.4:-35368630 MS.gene64394:CDS
CCTCTTTCTTTCACCACTTG+TGG 0.470663 2.4:-35369199 MS.gene64394:CDS
TCCGAACAGCTGCTGTTCTT+CGG 0.473876 2.4:-35368226 MS.gene64394:CDS
CAATGATATCTATGATATCT+GGG 0.474517 2.4:-35369142 MS.gene64394:CDS
CGACGACGATGGCGCTAACA+AGG 0.474648 2.4:+35369305 None:intergenic
GAGAAGATCCAAATACCAAT+TGG 0.476259 2.4:-35368422 MS.gene64394:CDS
ACACCTCTAGCAGTTCGCTT+TGG 0.480585 2.4:-35368018 MS.gene64394:CDS
TCGAATTGTTTGTTCCGTAT+GGG 0.486272 2.4:-35368484 MS.gene64394:CDS
ATCTCTCAAGGATCTTTCGT+TGG 0.488205 2.4:+35368911 None:intergenic
TTTACTTTATGACTCGTCCT+AGG 0.488605 2.4:-35369053 MS.gene64394:CDS
ACCGAAGAACAGCAGCTGTT+CGG 0.501657 2.4:+35368225 None:intergenic
GAAGACGACAATGGCAAAGT+TGG 0.504336 2.4:-35368337 MS.gene64394:CDS
AACATAATGAGTTACAATCT+TGG 0.513615 2.4:-35368652 MS.gene64394:CDS
ATTGTTTGTTCCGTATGGGA+GGG 0.513671 2.4:-35368480 MS.gene64394:CDS
TCAAAAGCTAGCTTGAAATC+CGG 0.514010 2.4:+35368154 None:intergenic
ATCTAAAACAGCTCTTCCAC+CGG 0.515874 2.4:+35368114 None:intergenic
GCCAAGGGAAGGATCAAGAA+AGG 0.516116 2.4:-35367974 MS.gene64394:CDS
GAGTTACAATCTTGGTGGGA+TGG 0.516163 2.4:-35368644 MS.gene64394:CDS
GAAATTCTTGGAACATCGAC+CGG 0.516259 2.4:+35367827 None:intergenic
ATCACGTTGAATTGGTATCC+CGG 0.518078 2.4:-35368529 MS.gene64394:CDS
AGCTTGGATCGATCGTTCCC+GGG 0.518481 2.4:+35368511 None:intergenic
TTGTTTGTTCCGTATGGGAG+GGG 0.520242 2.4:-35368479 MS.gene64394:CDS
CAAACAATTCGAAACAAGCT+TGG 0.524897 2.4:+35368495 None:intergenic
CTCCAAGACCAGATCTCTCA+AGG 0.527098 2.4:+35368899 None:intergenic
GTGCAACAGCGCAGTATGGA+AGG 0.529765 2.4:-35367912 MS.gene64394:CDS
CCACACCGTTCGCACTAACA+AGG 0.531499 2.4:-35368398 MS.gene64394:CDS
TCTCCTATCCGATCCCCTGT+TGG 0.533862 2.4:+35368441 None:intergenic
CTACAAAAGAGAAGAACCCT+TGG 0.534529 2.4:-35367871 MS.gene64394:CDS
GAGGGCGAGTGGTTTCTTCA+CGG 0.536357 2.4:-35368950 MS.gene64394:CDS
AGTCTCGCAAATGCACTAAG+AGG 0.536367 2.4:-35368990 MS.gene64394:CDS
ATTGCAGAGAGTGACGGAGT+TGG 0.537030 2.4:+35368703 None:intergenic
TCTACTTTCCAACAGGGGAT+CGG 0.537137 2.4:-35368449 MS.gene64394:CDS
AATTGTTTGTTCCGTATGGG+AGG 0.537893 2.4:-35368481 MS.gene64394:CDS
TCTTGCTAAAGAGCTTCTCC+AGG 0.541294 2.4:-35368593 MS.gene64394:CDS
GTATGCGTTGGTTGTTAGCA+TGG 0.541573 2.4:-35368557 MS.gene64394:CDS
GATATCATAGATATCATTGA+GGG 0.541632 2.4:+35369146 None:intergenic
GCAGCTGTTCGGACGTTGGA+AGG 0.548127 2.4:+35368236 None:intergenic
TTTCAAGGCATCACCTGCAA+CGG 0.548387 2.4:+35368288 None:intergenic
CCACAAGTGGTGAAAGAAAG+AGG 0.552043 2.4:+35369199 None:intergenic
GAACATTTCTGCATTCATGC+CGG 0.553989 2.4:-35368133 MS.gene64394:CDS
GAGAGTCGAGCAAATAATAA+CGG 0.554120 2.4:+35369095 None:intergenic
AGTTACAATCTTGGTGGGAT+GGG 0.556990 2.4:-35368643 MS.gene64394:CDS
AGCACCCTTGTTAGTGCGAA+CGG 0.557174 2.4:+35368393 None:intergenic
TGGTGGATCTCATCGATCCA+AGG 0.558878 2.4:+35367854 None:intergenic
TTAACAAGGTAGACTGGCCT+AGG 0.559143 2.4:+35369036 None:intergenic
TCATCCTAACTCGTATGCGT+TGG 0.563252 2.4:-35368569 MS.gene64394:CDS
AACAGCAGCTGTTCGGACGT+TGG 0.563575 2.4:+35368232 None:intergenic
ATTGGCTTACACGGAAGCCA+AGG 0.568482 2.4:-35367990 MS.gene64394:CDS
AAAACTTGAAGCTGTCTCCA+TGG 0.569815 2.4:-35368081 MS.gene64394:CDS
TTTCCAACAGGGGATCGGAT+AGG 0.573244 2.4:-35368444 MS.gene64394:CDS
AAATTGAAGTATGTGAAGCT+TGG 0.574482 2.4:-35369252 MS.gene64394:CDS
GAATTGCAGCCCCTCCCATA+CGG 0.574490 2.4:+35368470 None:intergenic
GAAAACAAACATTACCACGT+TGG 0.574589 2.4:-35368269 MS.gene64394:CDS
ACGGTAAAGCCTAAAGACAT+TGG 0.576657 2.4:-35368757 MS.gene64394:CDS
GAGAAATTAACAAGGTAGAC+TGG 0.577191 2.4:+35369030 None:intergenic
CATACGAGTTAGGATGAACC+TGG 0.577273 2.4:+35368575 None:intergenic
TTGGTACGAATTGGCTTACA+CGG 0.578204 2.4:-35367999 MS.gene64394:CDS
TCACGTTGAATTGGTATCCC+GGG 0.579569 2.4:-35368528 MS.gene64394:CDS
TAATTTCTCGTGTTACAAGC+CGG 0.580512 2.4:-35369016 MS.gene64394:CDS
TATCATTACCTAATCACTCA+TGG 0.585224 2.4:-35369228 MS.gene64394:CDS
GGAAGGATCCGCGACGACGA+TGG 0.585504 2.4:+35369294 None:intergenic
TCTTTCATTGAAGGATTGGG+AGG 0.589318 2.4:+35368841 None:intergenic
CATTTCTGCATTCATGCCGG+TGG 0.590314 2.4:-35368130 MS.gene64394:CDS
ATCCTTCAATGAAAGAAGCT+AGG 0.592042 2.4:-35368834 MS.gene64394:CDS
ATGGATCACTCGAGGGCGAG+TGG 0.595528 2.4:-35368961 MS.gene64394:CDS
ATGATCATTGCAGAGAGTGA+CGG 0.597131 2.4:+35368697 None:intergenic
GAACATCGACCGGAAACTGG+TGG 0.598498 2.4:+35367837 None:intergenic
ATAATGAGTTACAATCTTGG+TGG 0.599058 2.4:-35368649 MS.gene64394:CDS
TAATGAGTTACAATCTTGGT+GGG 0.599305 2.4:-35368648 MS.gene64394:CDS
GGTGGATCTCATCGATCCAA+GGG 0.617008 2.4:+35367855 None:intergenic
GTACCAAAGCGAACTGCTAG+AGG 0.619086 2.4:+35368015 None:intergenic
AGATATCATAGATATCATTG+AGG 0.623017 2.4:+35369145 None:intergenic
GCTGCAATTAACACCACAAG+TGG 0.623028 2.4:+35369186 None:intergenic
TGCTTAGAACAGCTTCTGGT+AGG 0.624810 2.4:+35368866 None:intergenic
GGATATTTATGGATCACTCG+AGG 0.626023 2.4:-35368969 MS.gene64394:CDS
CCTTGTTAGTGCGAACGGTG+TGG 0.627883 2.4:+35368398 None:intergenic
TCAAGAAAGGAGATAGAACA+TGG 0.636177 2.4:-35367961 MS.gene64394:CDS
AAACAAACATTACCACGTTG+GGG 0.638025 2.4:-35368267 MS.gene64394:CDS
AGAAGCTAGGAAAGAAGCGG+AGG 0.638732 2.4:-35368821 MS.gene64394:CDS
CTTGACGGCTCCATATGCCA+TGG 0.639071 2.4:+35368064 None:intergenic
TCGGACGTTGGAAGGACAAG+TGG 0.640146 2.4:+35368244 None:intergenic
GACGACGATGGCGCTAACAA+GGG 0.641367 2.4:+35369306 None:intergenic
TGCAATTCTACTTTCCAACA+GGG 0.643744 2.4:-35368455 MS.gene64394:CDS
GATATTTATGGATCACTCGA+GGG 0.648472 2.4:-35368968 MS.gene64394:CDS
TTGGCTTACACGGAAGCCAA+GGG 0.648617 2.4:-35367989 MS.gene64394:CDS
CTTACACGGAAGCCAAGGGA+AGG 0.652154 2.4:-35367985 MS.gene64394:CDS
GAAAGAAGCTAGGAAAGAAG+CGG 0.654182 2.4:-35368824 MS.gene64394:CDS
ATCAATCACTATAAGCTGAG+AGG 0.664913 2.4:-35368676 MS.gene64394:CDS
GCAATTCTACTTTCCAACAG+GGG 0.671034 2.4:-35368454 MS.gene64394:CDS
TGTTAGCGCCATCGTCGTCG+CGG 0.673997 2.4:-35369302 MS.gene64394:CDS
CACACCGTTCGCACTAACAA+GGG 0.677053 2.4:-35368397 MS.gene64394:CDS
ACACAAGAAGAAGACGACAA+TGG 0.692603 2.4:-35368346 MS.gene64394:CDS
GGCGAGTGGTTTCTTCACGG+AGG 0.715615 2.4:-35368947 MS.gene64394:CDS
TGTAACACGAGAAATTAACA+AGG 0.752828 2.4:+35369022 None:intergenic
AAGGACAAGTGGCCCCAACG+TGG 0.761089 2.4:+35368255 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTTTATCAAAAGATCTTA+TGG - chr2.4:35368836-35368855 MS.gene64394:CDS 20.0%
! AACATAATGAGTTACAATCT+TGG - chr2.4:35368495-35368514 MS.gene64394:CDS 25.0%
! AGATATCATAGATATCATTG+AGG + chr2.4:35368005-35368024 None:intergenic 25.0%
! CAATGATATCTATGATATCT+GGG - chr2.4:35368005-35368024 MS.gene64394:CDS 25.0%
! GATATCATAGATATCATTGA+GGG + chr2.4:35368004-35368023 None:intergenic 25.0%
! TCAATGATATCTATGATATC+TGG - chr2.4:35368004-35368023 MS.gene64394:CDS 25.0%
!!! TTCACTGATTTTTTGAAATC+TGG + chr2.4:35367877-35367896 None:intergenic 25.0%
AAAACAAACATTACCACGTT+GGG - chr2.4:35368879-35368898 MS.gene64394:CDS 30.0%
AAAAGGTCGATAAAAACACA+AGG + chr2.4:35368077-35368096 None:intergenic 30.0%
AAATTGAAGTATGTGAAGCT+TGG - chr2.4:35367895-35367914 MS.gene64394:CDS 30.0%
ATAATGAGTTACAATCTTGG+TGG - chr2.4:35368498-35368517 MS.gene64394:CDS 30.0%
ATCAGAATTCCAATGTCTTT+AGG + chr2.4:35368402-35368421 None:intergenic 30.0%
ATGCACTAAGAGGATATTTA+TGG - chr2.4:35368167-35368186 MS.gene64394:CDS 30.0%
TAATGAGTTACAATCTTGGT+GGG - chr2.4:35368499-35368518 MS.gene64394:CDS 30.0%
TATCATTACCTAATCACTCA+TGG - chr2.4:35367919-35367938 MS.gene64394:CDS 30.0%
TGTAACACGAGAAATTAACA+AGG + chr2.4:35368128-35368147 None:intergenic 30.0%
! AGAATGACACTTTATCGTTT+TGG - chr2.4:35369104-35369123 MS.gene64394:CDS 30.0%
! GAAGATAAAGCCTTATATTC+CGG - chr2.4:35368974-35368993 MS.gene64394:CDS 30.0%
!!! CTGATTTTTTGAAATCTGGA+AGG + chr2.4:35367873-35367892 None:intergenic 30.0%
AAACAAACATTACCACGTTG+GGG - chr2.4:35368880-35368899 MS.gene64394:CDS 35.0%
AGAACATGGCAAATAGCATT+TGG - chr2.4:35369200-35369219 MS.gene64394:CDS 35.0%
AGCTTCTTTCATTGAAGGAT+TGG + chr2.4:35368313-35368332 None:intergenic 35.0%
AGCTTGAAATCCGGAATATA+AGG + chr2.4:35368987-35369006 None:intergenic 35.0%
AGGACGAGTCATAAAGTAAA+AGG + chr2.4:35368094-35368113 None:intergenic 35.0%
ATCAATCACTATAAGCTGAG+AGG - chr2.4:35368471-35368490 MS.gene64394:CDS 35.0%
ATCCTTCAATGAAAGAAGCT+AGG - chr2.4:35368313-35368332 MS.gene64394:CDS 35.0%
CAAACAATTCGAAACAAGCT+TGG + chr2.4:35368655-35368674 None:intergenic 35.0%
GAAAACAAACATTACCACGT+TGG - chr2.4:35368878-35368897 MS.gene64394:CDS 35.0%
GAGAAATTAACAAGGTAGAC+TGG + chr2.4:35368120-35368139 None:intergenic 35.0%
GAGAAGATCCAAATACCAAT+TGG - chr2.4:35368725-35368744 MS.gene64394:CDS 35.0%
GAGAGTCGAGCAAATAATAA+CGG + chr2.4:35368055-35368074 None:intergenic 35.0%
GATATTTATGGATCACTCGA+GGG - chr2.4:35368179-35368198 MS.gene64394:CDS 35.0%
GCTTCTTTCATTGAAGGATT+GGG + chr2.4:35368312-35368331 None:intergenic 35.0%
TAATTTCTCGTGTTACAAGC+CGG - chr2.4:35368131-35368150 MS.gene64394:CDS 35.0%
TCAAGAAAGGAGATAGAACA+TGG - chr2.4:35369186-35369205 MS.gene64394:CDS 35.0%
TCGAATTGTTTGTTCCGTAT+GGG - chr2.4:35368663-35368682 MS.gene64394:CDS 35.0%
TGCAATTCTACTTTCCAACA+GGG - chr2.4:35368692-35368711 MS.gene64394:CDS 35.0%
TTCCTAGCTTCTTTCATTGA+AGG + chr2.4:35368318-35368337 None:intergenic 35.0%
TTCGAATTGTTTGTTCCGTA+TGG - chr2.4:35368662-35368681 MS.gene64394:CDS 35.0%
TTTACTTTATGACTCGTCCT+AGG - chr2.4:35368094-35368113 MS.gene64394:CDS 35.0%
! CGATAAAGTGTCATTCTTGA+CGG + chr2.4:35369101-35369120 None:intergenic 35.0%
! TCAAAAGCTAGCTTGAAATC+CGG + chr2.4:35368996-35369015 None:intergenic 35.0%
!! CGTGGTAATGTTTGTTTTCA+AGG + chr2.4:35368877-35368896 None:intergenic 35.0%
!! TGAGCTGTTTTCAAAGACAA+CGG - chr2.4:35368371-35368390 MS.gene64394:CDS 35.0%
AAAACTTGAAGCTGTCTCCA+TGG - chr2.4:35369066-35369085 MS.gene64394:CDS 40.0%
AATTGTTTGTTCCGTATGGG+AGG - chr2.4:35368666-35368685 MS.gene64394:CDS 40.0%
ACACAAGAAGAAGACGACAA+TGG - chr2.4:35368801-35368820 MS.gene64394:CDS 40.0%
ACGGTAAAGCCTAAAGACAT+TGG - chr2.4:35368390-35368409 MS.gene64394:CDS 40.0%
AGTTACAATCTTGGTGGGAT+GGG - chr2.4:35368504-35368523 MS.gene64394:CDS 40.0%
ATCACGTTGAATTGGTATCC+CGG - chr2.4:35368618-35368637 MS.gene64394:CDS 40.0%
ATCTCTCAAGGATCTTTCGT+TGG + chr2.4:35368239-35368258 None:intergenic 40.0%
ATGATCATTGCAGAGAGTGA+CGG + chr2.4:35368453-35368472 None:intergenic 40.0%
ATTGTTTGTTCCGTATGGGA+GGG - chr2.4:35368667-35368686 MS.gene64394:CDS 40.0%
CTACAAAAGAGAAGAACCCT+TGG - chr2.4:35369276-35369295 MS.gene64394:CDS 40.0%
CTGCAATTCTACTTTCCAAC+AGG - chr2.4:35368691-35368710 MS.gene64394:CDS 40.0%
GAAAGAAGCTAGGAAAGAAG+CGG - chr2.4:35368323-35368342 MS.gene64394:CDS 40.0%
GAAATTCTTGGAACATCGAC+CGG + chr2.4:35369323-35369342 None:intergenic 40.0%
GAACATTTCTGCATTCATGC+CGG - chr2.4:35369014-35369033 MS.gene64394:CDS 40.0%
GCAATTCTACTTTCCAACAG+GGG - chr2.4:35368693-35368712 MS.gene64394:CDS 40.0%
GGATATTTATGGATCACTCG+AGG - chr2.4:35368178-35368197 MS.gene64394:CDS 40.0%
TCTTTCATTGAAGGATTGGG+AGG + chr2.4:35368309-35368328 None:intergenic 40.0%
TGGAGAACATCACGTTGAAT+TGG - chr2.4:35368610-35368629 MS.gene64394:CDS 40.0%
! ATCTAAAACAGCTCTTCCAC+CGG + chr2.4:35369036-35369055 None:intergenic 40.0%
! CAAGGGTTCTTCTCTTTTGT+AGG + chr2.4:35369278-35369297 None:intergenic 40.0%
! TGGCAAATAGCATTTGGTTC+TGG - chr2.4:35369206-35369225 MS.gene64394:CDS 40.0%
! TTGGTACGAATTGGCTTACA+CGG - chr2.4:35369148-35369167 MS.gene64394:CDS 40.0%
AAAGATCTTATGGCCGTTGC+AGG - chr2.4:35368846-35368865 MS.gene64394:CDS 45.0%
ACAACCAACGCATACGAGTT+AGG + chr2.4:35368585-35368604 None:intergenic 45.0%
AGTCTCGCAAATGCACTAAG+AGG - chr2.4:35368157-35368176 MS.gene64394:CDS 45.0%
ATCCTTGAGAGATCTGGTCT+TGG - chr2.4:35368246-35368265 MS.gene64394:CDS 45.0%
CAGTTCGCTTTGGTACGAAT+TGG - chr2.4:35369139-35369158 MS.gene64394:CDS 45.0%
CATACGAGTTAGGATGAACC+TGG + chr2.4:35368575-35368594 None:intergenic 45.0%
CCACAAGTGGTGAAAGAAAG+AGG + chr2.4:35367951-35367970 None:intergenic 45.0%
CCTCTTTCTTTCACCACTTG+TGG - chr2.4:35367948-35367967 MS.gene64394:CDS 45.0%
CGAAAGATCCTTGAGAGATC+TGG - chr2.4:35368240-35368259 MS.gene64394:CDS 45.0%
CTGTTCTTCGGTACGTTAGT+TGG - chr2.4:35368933-35368952 MS.gene64394:CDS 45.0%
GAAGACGACAATGGCAAAGT+TGG - chr2.4:35368810-35368829 MS.gene64394:CDS 45.0%
GAGTTACAATCTTGGTGGGA+TGG - chr2.4:35368503-35368522 MS.gene64394:CDS 45.0%
GCTGCAATTAACACCACAAG+TGG + chr2.4:35367964-35367983 None:intergenic 45.0%
GGAATGCTTAGAACAGCTTC+TGG + chr2.4:35368288-35368307 None:intergenic 45.0%
GGTACATGCCATGAGTGATT+AGG + chr2.4:35367930-35367949 None:intergenic 45.0%
GGTGTGGACCAATTGGTATT+TGG + chr2.4:35368736-35368755 None:intergenic 45.0%
TCACGTTGAATTGGTATCCC+GGG - chr2.4:35368619-35368638 MS.gene64394:CDS 45.0%
TCATCCTAACTCGTATGCGT+TGG - chr2.4:35368578-35368597 MS.gene64394:CDS 45.0%
TCCTTTCTTGATCCTTCCCT+TGG + chr2.4:35369177-35369196 None:intergenic 45.0%
TCTACTTTCCAACAGGGGAT+CGG - chr2.4:35368698-35368717 MS.gene64394:CDS 45.0%
TCTTGCTAAAGAGCTTCTCC+AGG - chr2.4:35368554-35368573 MS.gene64394:CDS 45.0%
TGCTTAGAACAGCTTCTGGT+AGG + chr2.4:35368284-35368303 None:intergenic 45.0%
TTGTTTGTTCCGTATGGGAG+GGG - chr2.4:35368668-35368687 MS.gene64394:CDS 45.0%
! TTTCAAGGCATCACCTGCAA+CGG + chr2.4:35368862-35368881 None:intergenic 45.0%
!! GTATGCGTTGGTTGTTAGCA+TGG - chr2.4:35368590-35368609 MS.gene64394:CDS 45.0%
!! TTAACAAGGTAGACTGGCCT+AGG + chr2.4:35368114-35368133 None:intergenic 45.0%
AAGCTTGGATCGATCGTTCC+CGG + chr2.4:35368640-35368659 None:intergenic 50.0%
ACACCTCTAGCAGTTCGCTT+TGG - chr2.4:35369129-35369148 MS.gene64394:CDS 50.0%
ACCGAAGAACAGCAGCTGTT+CGG + chr2.4:35368925-35368944 None:intergenic 50.0%
AGAAGCTAGGAAAGAAGCGG+AGG - chr2.4:35368326-35368345 MS.gene64394:CDS 50.0%
AGCACCCTTGTTAGTGCGAA+CGG + chr2.4:35368757-35368776 None:intergenic 50.0%
CACACCGTTCGCACTAACAA+GGG - chr2.4:35368750-35368769 MS.gene64394:CDS 50.0%
CATTTCTGCATTCATGCCGG+TGG - chr2.4:35369017-35369036 MS.gene64394:CDS 50.0%
CGATGAGATCCACCAGTTTC+CGG - chr2.4:35369301-35369320 MS.gene64394:CDS 50.0%
CTCCAAGACCAGATCTCTCA+AGG + chr2.4:35368251-35368270 None:intergenic 50.0%
GAAGCGGAGGCAGTTATGTT+TGG - chr2.4:35368339-35368358 MS.gene64394:CDS 50.0%
GAAGCTGTCTCCATGGCATA+TGG - chr2.4:35369073-35369092 MS.gene64394:CDS 50.0%
GCCAAGGGAAGGATCAAGAA+AGG - chr2.4:35369173-35369192 MS.gene64394:CDS 50.0%
GGTGGATCTCATCGATCCAA+GGG + chr2.4:35369295-35369314 None:intergenic 50.0%
GTACCAAAGCGAACTGCTAG+AGG + chr2.4:35369135-35369154 None:intergenic 50.0%
GTGCATTTGCGAGACTCTTC+CGG + chr2.4:35368153-35368172 None:intergenic 50.0%
TCAAGTGCAACAGCGCAGTA+TGG - chr2.4:35369231-35369250 MS.gene64394:CDS 50.0%
TCCGAACAGCTGCTGTTCTT+CGG - chr2.4:35368921-35368940 MS.gene64394:CDS 50.0%
TTGGAACATCGACCGGAAAC+TGG + chr2.4:35369316-35369335 None:intergenic 50.0%
TTGGCTTACACGGAAGCCAA+GGG - chr2.4:35369158-35369177 MS.gene64394:CDS 50.0%
TTTCCAACAGGGGATCGGAT+AGG - chr2.4:35368703-35368722 MS.gene64394:CDS 50.0%
! ATTGCAGAGAGTGACGGAGT+TGG + chr2.4:35368447-35368466 None:intergenic 50.0%
! ATTGGCTTACACGGAAGCCA+AGG - chr2.4:35369157-35369176 MS.gene64394:CDS 50.0%
! TGGTGGATCTCATCGATCCA+AGG + chr2.4:35369296-35369315 None:intergenic 50.0%
AACAGCAGCTGTTCGGACGT+TGG + chr2.4:35368918-35368937 None:intergenic 55.0%
AGCTTGGATCGATCGTTCCC+GGG + chr2.4:35368639-35368658 None:intergenic 55.0%
CCACACCGTTCGCACTAACA+AGG - chr2.4:35368749-35368768 MS.gene64394:CDS 55.0%
CCTTGTTAGTGCGAACGGTG+TGG + chr2.4:35368752-35368771 None:intergenic 55.0%
CTTACACGGAAGCCAAGGGA+AGG - chr2.4:35369162-35369181 MS.gene64394:CDS 55.0%
CTTGACGGCTCCATATGCCA+TGG + chr2.4:35369086-35369105 None:intergenic 55.0%
GAACATCGACCGGAAACTGG+TGG + chr2.4:35369313-35369332 None:intergenic 55.0%
GAATTGCAGCCCCTCCCATA+CGG + chr2.4:35368680-35368699 None:intergenic 55.0%
GACGACGATGGCGCTAACAA+GGG + chr2.4:35367844-35367863 None:intergenic 55.0%
GAGGGCGAGTGGTTTCTTCA+CGG - chr2.4:35368197-35368216 MS.gene64394:CDS 55.0%
GCGCAGTATGGAAGGCTCTA+AGG - chr2.4:35369243-35369262 MS.gene64394:CDS 55.0%
GTGCAACAGCGCAGTATGGA+AGG - chr2.4:35369235-35369254 MS.gene64394:CDS 55.0%
TCGGACGTTGGAAGGACAAG+TGG + chr2.4:35368906-35368925 None:intergenic 55.0%
TCTCCTATCCGATCCCCTGT+TGG + chr2.4:35368709-35368728 None:intergenic 55.0%
TGCGAACGGTGTGGACCAAT+TGG + chr2.4:35368743-35368762 None:intergenic 55.0%
AAGGACAAGTGGCCCCAACG+TGG + chr2.4:35368895-35368914 None:intergenic 60.0%
ATGGATCACTCGAGGGCGAG+TGG - chr2.4:35368186-35368205 MS.gene64394:CDS 60.0%
CGACGACGATGGCGCTAACA+AGG + chr2.4:35367845-35367864 None:intergenic 60.0%
GCAGCTGTTCGGACGTTGGA+AGG + chr2.4:35368914-35368933 None:intergenic 60.0%
GGCGAGTGGTTTCTTCACGG+AGG - chr2.4:35368200-35368219 MS.gene64394:CDS 60.0%
GTGGGATGGGATGCAGTGCT+GGG - chr2.4:35368517-35368536 MS.gene64394:CDS 60.0%
TGTTAGCGCCATCGTCGTCG+CGG - chr2.4:35367845-35367864 MS.gene64394:CDS 60.0%
GGAAGGATCCGCGACGACGA+TGG + chr2.4:35367856-35367875 None:intergenic 65.0%
GGTGGGATGGGATGCAGTGC+TGG - chr2.4:35368516-35368535 MS.gene64394:CDS 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 35367817 35369352 35367817 ID=MS.gene64394
chr2.4 mRNA 35367817 35369352 35367817 ID=MS.gene64394.t1;Parent=MS.gene64394
chr2.4 exon 35367817 35369352 35367817 ID=MS.gene64394.t1.exon1;Parent=MS.gene64394.t1
chr2.4 CDS 35367817 35369352 35367817 ID=cds.MS.gene64394.t1;Parent=MS.gene64394.t1
Gene Sequence

>MS.gene64394

ATGACAGAATCAAAACAACATACACCCTTGTTAGCGCCATCGTCGTCGCGGATCCTTCCAGATTTCAAAAAATCAGTGAAATTGAAGTATGTGAAGCTTGGTTATCATTACCTAATCACTCATGGCATGTACCTCTTTCTTTCACCACTTGTGGTGTTAATTGCAGCTCAGTTATCAACTTTCTCCCTCAATGATATCTATGATATCTGGGAAAATCTGCAGTACAATCTCGTATCCGTTATTATTTGCTCGACTCTCCTTGTGTTTTTATCGACCTTTTACTTTATGACTCGTCCTAGGCCAGTCTACCTTGTTAATTTCTCGTGTTACAAGCCGGAAGAGTCTCGCAAATGCACTAAGAGGATATTTATGGATCACTCGAGGGCGAGTGGTTTCTTCACGGAGGAGAATCTTGATTTCCAACGAAAGATCCTTGAGAGATCTGGTCTTGGAGAAAACACTTACCTACCAGAAGCTGTTCTAAGCATTCCTCCCAATCCTTCAATGAAAGAAGCTAGGAAAGAAGCGGAGGCAGTTATGTTTGGTGCTATCGATGAGCTGTTTTCAAAGACAACGGTAAAGCCTAAAGACATTGGAATTCTGATTGTGAATTGTAGTCTTTTCTGTCCAACTCCGTCACTCTCTGCAATGATCATCAATCACTATAAGCTGAGAGGTAACATAATGAGTTACAATCTTGGTGGGATGGGATGCAGTGCTGGGATTATTTCAATTGATCTTGCTAAAGAGCTTCTCCAGGTTCATCCTAACTCGTATGCGTTGGTTGTTAGCATGGAGAACATCACGTTGAATTGGTATCCCGGGAACGATCGATCCAAGCTTGTTTCGAATTGTTTGTTCCGTATGGGAGGGGCTGCAATTCTACTTTCCAACAGGGGATCGGATAGGAGAAGATCCAAATACCAATTGGTCCACACCGTTCGCACTAACAAGGGTGCTGATGACAAGTGCTTCAGCTGCGTTACACAAGAAGAAGACGACAATGGCAAAGTTGGTGTTACTTTATCAAAAGATCTTATGGCCGTTGCAGGTGATGCCTTGAAAACAAACATTACCACGTTGGGGCCACTTGTCCTTCCAACGTCCGAACAGCTGCTGTTCTTCGGTACGTTAGTTGGAAAGAAACTCTTCAAGATGAAGATAAAGCCTTATATTCCGGATTTCAAGCTAGCTTTTGAACATTTCTGCATTCATGCCGGTGGAAGAGCTGTTTTAGATGAGCTAGAGAAAAACTTGAAGCTGTCTCCATGGCATATGGAGCCGTCAAGAATGACACTTTATCGTTTTGGAAACACCTCTAGCAGTTCGCTTTGGTACGAATTGGCTTACACGGAAGCCAAGGGAAGGATCAAGAAAGGAGATAGAACATGGCAAATAGCATTTGGTTCTGGATTCAAGTGCAACAGCGCAGTATGGAAGGCTCTAAGGACAATCAATCCTACAAAAGAGAAGAACCCTTGGATCGATGAGATCCACCAGTTTCCGGTCGATGTTCCAAGAATTTCTGCCATCTAA
Protein sequence

>MS.gene64394.t1

MTESKQHTPLLAPSSSRILPDFKKSVKLKYVKLGYHYLITHGMYLFLSPLVVLIAAQLSTFSLNDIYDIWENLQYNLVSVIICSTLLVFLSTFYFMTRPRPVYLVNFSCYKPEESRKCTKRIFMDHSRASGFFTEENLDFQRKILERSGLGENTYLPEAVLSIPPNPSMKEARKEAEAVMFGAIDELFSKTTVKPKDIGILIVNCSLFCPTPSLSAMIINHYKLRGNIMSYNLGGMGCSAGIISIDLAKELLQVHPNSYALVVSMENITLNWYPGNDRSKLVSNCLFRMGGAAILLSNRGSDRRRSKYQLVHTVRTNKGADDKCFSCVTQEEDDNGKVGVTLSKDLMAVAGDALKTNITTLGPLVLPTSEQLLFFGTLVGKKLFKMKIKPYIPDFKLAFEHFCIHAGGRAVLDELEKNLKLSPWHMEPSRMTLYRFGNTSSSSLWYELAYTEAKGRIKKGDRTWQIAFGSGFKCNSAVWKALRTINPTKEKNPWIDEIHQFPVDVPRISAI