AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65114


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65114.t1 MTR_7g058640 93.846 195 12 0 1 195 1 195 3.26e-138 385
MS.gene65114.t1 MTR_7g058640 95.930 172 7 0 1 172 1 172 1.19e-123 348
MS.gene65114.t1 MTR_4g022570 74.872 195 49 0 1 195 1 195 2.67e-111 318
MS.gene65114.t1 MTR_1g047720 66.197 213 72 0 1 213 1 213 4.68e-111 317
MS.gene65114.t1 MTR_7g058900 70.769 195 57 0 1 195 1 195 2.72e-104 300
MS.gene65114.t1 MTR_4g083490 67.358 193 63 0 3 195 5 197 5.98e-101 291
MS.gene65114.t1 MTR_2g436870 61.033 213 83 0 1 213 1 213 1.52e-100 290
MS.gene65114.t1 MTR_2g028790 65.803 193 66 0 3 195 7 199 2.25e-97 282
MS.gene65114.t1 MTR_1g111890 47.619 231 102 2 1 212 1 231 3.58e-80 239
MS.gene65114.t1 MTR_7g058640 94.017 117 7 0 79 195 1 117 6.84e-77 227
MS.gene65114.t1 MTR_1g047710 60.902 133 51 1 56 187 3 135 3.37e-57 177
MS.gene65114.t1 MTR_4g022470 71.028 107 31 0 64 170 10 116 1.50e-52 165
MS.gene65114.t1 MTR_5g089370 36.649 191 118 3 5 194 2 190 2.82e-38 132
MS.gene65114.t1 MTR_4g052550 32.461 191 126 3 5 194 2 190 1.45e-36 127
MS.gene65114.t1 MTR_4g115390 29.524 210 141 4 6 210 3 210 3.16e-29 108
MS.gene65114.t1 MTR_4g022550 71.053 38 11 0 158 195 10 47 1.82e-12 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65114.t1 AT2G33120 72.821 195 53 0 1 195 1 195 7.80e-108 309
MS.gene65114.t1 AT1G04760 69.000 200 62 0 1 200 1 200 9.28e-108 309
MS.gene65114.t1 AT1G04760 69.000 200 62 0 1 200 1 200 9.28e-108 309
MS.gene65114.t1 AT1G04750 72.308 195 54 0 1 195 1 195 2.18e-107 308
MS.gene65114.t1 AT2G33120 69.458 203 54 1 1 195 1 203 1.24e-104 301
MS.gene65114.t1 AT2G32670 69.744 195 59 0 1 195 66 260 2.28e-104 303
MS.gene65114.t1 AT4G15780 61.202 183 70 1 1 182 1 183 2.99e-83 245
MS.gene65114.t1 AT2G33110 60.513 195 73 1 1 195 1 191 5.21e-83 246
MS.gene65114.t1 AT3G54300 48.069 233 98 2 1 212 1 231 2.73e-79 237
MS.gene65114.t1 AT3G54300 48.069 233 98 2 1 212 1 231 2.73e-79 237
MS.gene65114.t1 AT3G54300 48.069 233 98 2 1 212 1 231 2.73e-79 237
MS.gene65114.t1 AT2G33110 63.095 168 62 0 1 168 1 168 2.28e-76 228
MS.gene65114.t1 AT1G04750 70.940 117 34 0 79 195 1 117 1.39e-58 181
MS.gene65114.t1 AT1G04750 70.940 117 34 0 79 195 1 117 1.39e-58 181
MS.gene65114.t1 AT1G04750 70.940 117 34 0 79 195 1 117 1.39e-58 181
MS.gene65114.t1 AT5G11150 33.649 211 133 4 5 213 2 207 3.66e-39 134
MS.gene65114.t1 AT5G22360 32.536 209 136 4 5 211 2 207 4.25e-39 134
MS.gene65114.t1 AT5G11150 33.649 211 133 4 5 213 2 207 6.16e-39 134
MS.gene65114.t1 AT4G32150 34.211 190 122 3 5 193 2 189 8.70e-35 123
MS.gene65114.t1 AT2G25340 32.105 190 126 3 5 193 2 189 3.52e-32 116
MS.gene65114.t1 AT2G33120 67.742 62 20 0 134 195 52 113 4.65e-22 88.2
MS.gene65114.t1 AT3G24890 45.745 94 45 2 113 200 14 107 2.77e-18 77.8

Find 41 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGCTTGAATAAAGAATT+TGG 0.138378 7.1:-42775882 MS.gene65114:CDS
CTTTGCAGGCACAAGATTTA+AGG 0.164312 7.1:-42775188 MS.gene65114:intron
GCAAGTTTGCTAACCTCTTC+TGG 0.229459 7.1:+42775715 None:intergenic
CAATCTTCACTTGACGATTA+AGG 0.242055 7.1:+42775540 None:intergenic
AGCTATTTCAGCAAAGTTTC+CGG 0.255031 7.1:+42776387 None:intergenic
TGCATATGCTCCTTCAATTT+CGG 0.281591 7.1:+42775754 None:intergenic
GGCGCGTGGCACGGTGATTC+TGG 0.320729 7.1:-42776428 MS.gene65114:CDS
TGGTTGCTGGGAGTCTTTGC+AGG 0.323885 7.1:+42776356 None:intergenic
AAAGCTTGAATAAAGAATTT+GGG 0.394970 7.1:-42775881 MS.gene65114:intron
TCCGGTGAAGTTGGTGTGCT+CGG 0.407070 7.1:+42776405 None:intergenic
AACTGTCAACCTTCGTGATC+AGG 0.421098 7.1:-42775501 MS.gene65114:intron
TTTGCAGGCACAAGATTTAA+GGG 0.423640 7.1:-42775187 MS.gene65114:intron
CAAGAAAGGCCATAGCAATA+TGG 0.430285 7.1:+42775967 None:intergenic
ATCACTTATATACAGCTTTG+TGG 0.443785 7.1:-42776449 MS.gene65114:CDS
TATGTTGGAAAATATTGATC+AGG 0.458760 7.1:-42775656 MS.gene65114:intron
CTCCTTGATGTGATCAAGAA+AGG 0.487927 7.1:+42775953 None:intergenic
AGCTTACTGCCTTGTTGCTG+TGG 0.489435 7.1:-42776003 MS.gene65114:CDS
TCAAGTTCAAGATGTTATGT+TGG 0.490196 7.1:-42775671 MS.gene65114:CDS
AGCAAAGTTTCCGGTGAAGT+TGG 0.500993 7.1:+42776396 None:intergenic
CAGCTTTGTGGCGCGTGGCA+CGG 0.506415 7.1:-42776437 MS.gene65114:CDS
GTAATTTAGACCGAAATTGA+AGG 0.508592 7.1:-42775764 MS.gene65114:intron
GGCAAGTACTGAATCCATCA+TGG 0.518155 7.1:+42776284 None:intergenic
GCCGAGCACACCAACTTCAC+CGG 0.518367 7.1:-42776406 MS.gene65114:CDS
GGCCTTTCTTGATCACATCA+AGG 0.528346 7.1:-42775955 MS.gene65114:CDS
TTCAACTACCTCGCCCATGA+TGG 0.533919 7.1:-42776298 MS.gene65114:CDS
ATCATGGGCGAGGTAGTTGA+AGG 0.542753 7.1:+42776300 None:intergenic
ACTTGCGAAAGTAAAAGCTC+AGG 0.550493 7.1:-42775698 MS.gene65114:CDS
TACTGAATCCATCATGGGCG+AGG 0.571090 7.1:+42776290 None:intergenic
ATATACAGCTTTGTGGCGCG+TGG 0.589934 7.1:-42776442 MS.gene65114:CDS
TTTACCAAAAGATATGGTGG+AGG 0.594903 7.1:-42775927 MS.gene65114:CDS
TGCAGGCACAAGATTTAAGG+GGG 0.606935 7.1:-42775185 MS.gene65114:intron
TTGCAGGCACAAGATTTAAG+GGG 0.610788 7.1:-42775186 MS.gene65114:intron
GGCAATTAAGGAGAAAGATG+TGG 0.621585 7.1:-42775155 MS.gene65114:CDS
CAAAAGACTCCACAGCAACA+AGG 0.623781 7.1:+42775994 None:intergenic
GTAGTTGAAGGTATGACCAT+CGG 0.631667 7.1:+42776312 None:intergenic
GCAAGTACTGAATCCATCAT+GGG 0.632742 7.1:+42776285 None:intergenic
TTGTGTGGAACATCCAGAAG+AGG 0.637264 7.1:-42775728 MS.gene65114:CDS
AAATTCACTTACAACACCGA+TGG 0.653817 7.1:-42776328 MS.gene65114:CDS
CTTTCTTGATCACATCAAGG+AGG 0.668827 7.1:-42775952 MS.gene65114:CDS
GGAGCATATGCAATATTGTG+TGG 0.672159 7.1:-42775743 MS.gene65114:CDS
ATAAAAGGACCTGATCACGA+AGG 0.753478 7.1:+42775492 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATGTAATTTAGTTATTTA+TGG + chr7.1:42776242-42776261 None:intergenic 15.0%
!!! TTTTTTTTATTGTATATGCA+TGG - chr7.1:42775748-42775767 MS.gene65114:CDS 15.0%
!! AAAAGCTTGAATAAAGAATT+TGG - chr7.1:42775652-42775671 MS.gene65114:intron 20.0%
!! AAAGCTTGAATAAAGAATTT+GGG - chr7.1:42775653-42775672 MS.gene65114:intron 20.0%
!! AGGTATATATATGTCTATAT+TGG - chr7.1:42775898-42775917 MS.gene65114:CDS 20.0%
!! TAAAAGAAAAAGATAAAACG+AGG + chr7.1:42775274-42775293 None:intergenic 20.0%
!! TTATAGTTATGTAACTGATA+TGG - chr7.1:42776312-42776331 MS.gene65114:CDS 20.0%
!! TTTATGGATGAACAAATAAA+AGG + chr7.1:42776060-42776079 None:intergenic 20.0%
!!! TCTTTTACATAATCTAATTG+TGG - chr7.1:42775287-42775306 MS.gene65114:intron 20.0%
! CAAATCATAAATTTCCTTAG+AGG + chr7.1:42775440-42775459 None:intergenic 25.0%
! TATGTTGGAAAATATTGATC+AGG - chr7.1:42775878-42775897 MS.gene65114:intron 25.0%
!! ATTTTTACACACACATGAAT+AGG - chr7.1:42776196-42776215 MS.gene65114:intron 25.0%
!! TTTTTACACACACATGAATA+GGG - chr7.1:42776197-42776216 MS.gene65114:intron 25.0%
!!! ATTTTATTTGTTTTGTCTGC+AGG - chr7.1:42775970-42775989 MS.gene65114:CDS 25.0%
ATCACTTATATACAGCTTTG+TGG - chr7.1:42775085-42775104 MS.gene65114:CDS 30.0%
GTAATTTAGACCGAAATTGA+AGG - chr7.1:42775770-42775789 MS.gene65114:intron 30.0%
TAACTGATATGGTTTGAATG+TGG - chr7.1:42776323-42776342 MS.gene65114:CDS 30.0%
TCAAATGCATTTACATGTAC+TGG - chr7.1:42775393-42775412 MS.gene65114:intron 30.0%
TCAAGTTCAAGATGTTATGT+TGG - chr7.1:42775863-42775882 MS.gene65114:intron 30.0%
! GAGGATTTTACCAAAAGATA+TGG - chr7.1:42775601-42775620 MS.gene65114:intron 30.0%
! GATTTTACCAAAAGATATGG+TGG - chr7.1:42775604-42775623 MS.gene65114:intron 30.0%
! GTTGTAAGTGAATTTTGTGT+TGG + chr7.1:42775201-42775220 None:intergenic 30.0%
! TTTTACACACACATGAATAG+GGG - chr7.1:42776198-42776217 MS.gene65114:intron 30.0%
!! AGTGAAGATTGATGTTTTGA+TGG - chr7.1:42776006-42776025 MS.gene65114:intron 30.0%
AAATTCACTTACAACACCGA+TGG - chr7.1:42775206-42775225 MS.gene65114:intron 35.0%
CAATCTTCACTTGACGATTA+AGG + chr7.1:42775997-42776016 None:intergenic 35.0%
TAGCTATGACACATGGTTTA+TGG + chr7.1:42776076-42776095 None:intergenic 35.0%
TATGTCTATATTGGATCCTC+TGG - chr7.1:42775907-42775926 MS.gene65114:CDS 35.0%
TGCATATGCTCCTTCAATTT+CGG + chr7.1:42775783-42775802 None:intergenic 35.0%
TTTACCAAAAGATATGGTGG+AGG - chr7.1:42775607-42775626 MS.gene65114:intron 35.0%
TTTGCAGGCACAAGATTTAA+GGG - chr7.1:42776347-42776366 MS.gene65114:CDS 35.0%
! AGCTATTTCAGCAAAGTTTC+CGG + chr7.1:42775150-42775169 None:intergenic 35.0%
! TTTTCCTCCACCATATCTTT+TGG + chr7.1:42775614-42775633 None:intergenic 35.0%
! TTTTGATCGCCATATTGCTA+TGG - chr7.1:42775558-42775577 MS.gene65114:intron 35.0%
!! TACATGTACTGGTTTCATGT+CGG - chr7.1:42775404-42775423 MS.gene65114:intron 35.0%
!!! TGAATTTTGTGTTGGTTGCT+GGG + chr7.1:42775193-42775212 None:intergenic 35.0%
AACGATCTGTGCTTCAAAGT+CGG + chr7.1:42775935-42775954 None:intergenic 40.0%
AAGATTTAAGGGGGAATGCA+GGG - chr7.1:42776358-42776377 MS.gene65114:CDS 40.0%
ACTTGCGAAAGTAAAAGCTC+AGG - chr7.1:42775836-42775855 MS.gene65114:intron 40.0%
ATAAAAGGACCTGATCACGA+AGG + chr7.1:42776045-42776064 None:intergenic 40.0%
CAAGAAAGGCCATAGCAATA+TGG + chr7.1:42775570-42775589 None:intergenic 40.0%
CTCCTTGATGTGATCAAGAA+AGG + chr7.1:42775584-42775603 None:intergenic 40.0%
CTTTCTTGATCACATCAAGG+AGG - chr7.1:42775582-42775601 MS.gene65114:intron 40.0%
CTTTGCAGGCACAAGATTTA+AGG - chr7.1:42776346-42776365 MS.gene65114:CDS 40.0%
GGAGCATATGCAATATTGTG+TGG - chr7.1:42775791-42775810 MS.gene65114:intron 40.0%
GGCAATTAAGGAGAAAGATG+TGG - chr7.1:42776379-42776398 MS.gene65114:CDS 40.0%
TCATCACTAGCTATGACACA+TGG + chr7.1:42776083-42776102 None:intergenic 40.0%
TCGCAGAAAACATAGTACTG+TGG - chr7.1:42776127-42776146 MS.gene65114:intron 40.0%
TTGCAGGCACAAGATTTAAG+GGG - chr7.1:42776348-42776367 MS.gene65114:CDS 40.0%
! CAGTACTATGTTTTCTGCGA+TGG + chr7.1:42776127-42776146 None:intergenic 40.0%
! GTAGTTGAAGGTATGACCAT+CGG + chr7.1:42775225-42775244 None:intergenic 40.0%
!! GCAAGTACTGAATCCATCAT+GGG + chr7.1:42775252-42775271 None:intergenic 40.0%
!!! GTGAATTTTGTGTTGGTTGC+TGG + chr7.1:42775194-42775213 None:intergenic 40.0%
AACTGTCAACCTTCGTGATC+AGG - chr7.1:42776033-42776052 MS.gene65114:intron 45.0%
AGCAAAGTTTCCGGTGAAGT+TGG + chr7.1:42775141-42775160 None:intergenic 45.0%
CAAAAGACTCCACAGCAACA+AGG + chr7.1:42775543-42775562 None:intergenic 45.0%
CAAGATTTAAGGGGGAATGC+AGG - chr7.1:42776357-42776376 MS.gene65114:CDS 45.0%
GGCCTTTCTTGATCACATCA+AGG - chr7.1:42775579-42775598 MS.gene65114:intron 45.0%
TCGGACATTGAACACCTCTA+AGG - chr7.1:42775423-42775442 MS.gene65114:intron 45.0%
TGCAGGCACAAGATTTAAGG+GGG - chr7.1:42776349-42776368 MS.gene65114:CDS 45.0%
TTGTGTGGAACATCCAGAAG+AGG - chr7.1:42775806-42775825 MS.gene65114:intron 45.0%
! TGGTTTGAATGTGGCTTTGC+AGG - chr7.1:42776332-42776351 MS.gene65114:CDS 45.0%
!! GCAAGTTTGCTAACCTCTTC+TGG + chr7.1:42775822-42775841 None:intergenic 45.0%
!! GGCAAGTACTGAATCCATCA+TGG + chr7.1:42775253-42775272 None:intergenic 45.0%
ATCATGGGCGAGGTAGTTGA+AGG + chr7.1:42775237-42775256 None:intergenic 50.0%
TACTGAATCCATCATGGGCG+AGG + chr7.1:42775247-42775266 None:intergenic 50.0%
TTCAACTACCTCGCCCATGA+TGG - chr7.1:42775236-42775255 MS.gene65114:intron 50.0%
! AGCTTACTGCCTTGTTGCTG+TGG - chr7.1:42775531-42775550 MS.gene65114:CDS 50.0%
! ATATACAGCTTTGTGGCGCG+TGG - chr7.1:42775092-42775111 MS.gene65114:CDS 50.0%
GGGGGAATGCAGGGCAATTA+AGG - chr7.1:42776367-42776386 MS.gene65114:CDS 55.0%
TGGTTGCTGGGAGTCTTTGC+AGG + chr7.1:42775181-42775200 None:intergenic 55.0%
! TGCTTCAAAGTCGGCTCCAG+AGG + chr7.1:42775926-42775945 None:intergenic 55.0%
!! TCCGGTGAAGTTGGTGTGCT+CGG + chr7.1:42775132-42775151 None:intergenic 55.0%
GCCGAGCACACCAACTTCAC+CGG - chr7.1:42775128-42775147 MS.gene65114:CDS 60.0%
! CAGCTTTGTGGCGCGTGGCA+CGG - chr7.1:42775097-42775116 MS.gene65114:CDS 65.0%
GGCGCGTGGCACGGTGATTC+TGG - chr7.1:42775106-42775125 MS.gene65114:CDS 70.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 42775074 42776482 42775074 ID=MS.gene65114
chr7.1 mRNA 42775074 42776482 42775074 ID=MS.gene65114.t1;Parent=MS.gene65114
chr7.1 exon 42776293 42776482 42776293 ID=MS.gene65114.t1.exon1;Parent=MS.gene65114.t1
chr7.1 CDS 42776293 42776482 42776293 ID=cds.MS.gene65114.t1;Parent=MS.gene65114.t1
chr7.1 exon 42775882 42776023 42775882 ID=MS.gene65114.t1.exon2;Parent=MS.gene65114.t1
chr7.1 CDS 42775882 42776023 42775882 ID=cds.MS.gene65114.t1;Parent=MS.gene65114.t1
chr7.1 exon 42775657 42775777 42775657 ID=MS.gene65114.t1.exon3;Parent=MS.gene65114.t1
chr7.1 CDS 42775657 42775777 42775657 ID=cds.MS.gene65114.t1;Parent=MS.gene65114.t1
chr7.1 exon 42775502 42775564 42775502 ID=MS.gene65114.t1.exon4;Parent=MS.gene65114.t1
chr7.1 CDS 42775502 42775564 42775502 ID=cds.MS.gene65114.t1;Parent=MS.gene65114.t1
chr7.1 exon 42775074 42775202 42775074 ID=MS.gene65114.t1.exon5;Parent=MS.gene65114.t1
chr7.1 CDS 42775074 42775202 42775074 ID=cds.MS.gene65114.t1;Parent=MS.gene65114.t1
Gene Sequence

>MS.gene65114

ATGGCGCAACAATCACTTATATACAGCTTTGTGGCGCGTGGCACGGTGATTCTGGCCGAGCACACCAACTTCACCGGAAACTTTGCTGAAATAGCTTTGCAATGCCTGCAAAGACTCCCAGCAACCAACACAAAATTCACTTACAACACCGATGGTCATACCTTCAACTACCTCGCCCATGATGGATTCAGTACTTGCCTCGTTTTATCTTTTTCTTTTACATAATCTAATTGTGGAATAGCATATCAATGCAATGCGATATATGAGACACAGACACTAGTGTTTAGATCTTGATAATAATTTAAGAAAATATAATTGATCAAATGCATTTACATGTACTGGTTTCATGTCGGACATTGAACACCTCTAAGGAAATTTATGATTTGATTTATATATTGCTTTCTTTTTCTTTCTAAAATTCTACTATATTATTAGACTTACATGATCTATTATCTTTAGCTTACTGCCTTGTTGCTGTGGAGTCTTTTGATCGCCATATTGCTATGGCCTTTCTTGATCACATCAAGGAGGATTTTACCAAAAGATATGGTGGAGGAAAAGCTGCAACAGCAACATCTAAAAGCTTGAATAAAGAATTTGGGTACTCAAGTTTATAACAATTTTGTGTATAGAAAATGTGTCACAAAAGTTGAATTGCATTAATTAACATGAAGTTTTTTTTATTGTATATGCATGGTAATTTAGACCGAAATTGAAGGAGCATATGCAATATTGTGTGGAACATCCAGAAGAGGTTAGCAAACTTGCGAAAGTAAAAGCTCAGGTTTCTCAAGTTCAAGATGTTATGTTGGAAAATATTGATCAGGTATATATATGTCTATATTGGATCCTCTGGAGCCGACTTTGAAGCACAGATCGTTAGATTAAGAGAGAAAATTTTATTTGTTTTGTCTGCAGGTCCTTAATCGTCAAGTGAAGATTGATGTTTTGATGGACAAAACTGTCAACCTTCGTGATCAGGTCCTTTTATTTGTTCATCCATAAACCATGTGTCATAGCTAGTGATGAAATTTTCACAAAATCACAAGACCATCGCAGAAAACATAGTACTGTGGTTATTTATGACTGTTCTAGTTCATGAATTACTTACTATATATTTATATTTTTACACACACATGAATAGGGGTCAAGTATAATCTAGTAACCATAAATAACTAAATTACATTGTCAAATTATCTTAACAGATATATACACATAATTTTAAATAAATTAGACATTTATAGTTATGTAACTGATATGGTTTGAATGTGGCTTTGCAGGCACAAGATTTAAGGGGGAATGCAGGGCAATTAAGGAGAAAGATGTGGTTTCAGAACATGAAGATAAAACTGATAGTTCTTGCTATCATAATTGTCATCATTCTAATAATTGTTCTTGTTCTTACATAA

Protein sequence

>MS.gene65114.t1

MAQQSLIYSFVARGTVILAEHTNFTGNFAEIALQCLQRLPATNTKFTYNTDGHTFNYLAHDGFTYCLVAVESFDRHIAMAFLDHIKEDFTKRYGGGKAATATSKSLNKEFGPKLKEHMQYCVEHPEEVSKLAKVKAQVSQVQDVMLENIDQVLNRQVKIDVLMDKTVNLRDQAQDLRGNAGQLRRKMWFQNMKIKLIVLAIIIVIILIIVLVLT