Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65127.t1 | XP_024627009.1 | 98.7 | 393 | 4 | 1 | 1 | 392 | 1 | 393 | 1.80E-222 | 781.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65127.t1 | A0A072U0Z3 | 98.7 | 393 | 4 | 1 | 1 | 392 | 1 | 393 | 1.3e-222 | 781.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051147 | MS.gene65127 | 0.800754 | 1.24E-48 | -1.69E-46 |
| MS.gene051800 | MS.gene65127 | 0.808043 | 3.76E-50 | -1.69E-46 |
| MS.gene052674 | MS.gene65127 | 0.82734 | 1.65E-54 | -1.69E-46 |
| MS.gene052675 | MS.gene65127 | 0.823857 | 1.11E-53 | -1.69E-46 |
| MS.gene052677 | MS.gene65127 | 0.833933 | 4.01E-56 | -1.69E-46 |
| MS.gene05350 | MS.gene65127 | 0.816317 | 5.89E-52 | -1.69E-46 |
| MS.gene054479 | MS.gene65127 | 0.802443 | 5.60E-49 | -1.69E-46 |
| MS.gene056174 | MS.gene65127 | 0.804714 | 1.89E-49 | -1.69E-46 |
| MS.gene056480 | MS.gene65127 | 0.824881 | 6.35E-54 | -1.69E-46 |
| MS.gene057915 | MS.gene65127 | 0.805114 | 1.56E-49 | -1.69E-46 |
| MS.gene058081 | MS.gene65127 | 0.860367 | 2.17E-63 | -1.69E-46 |
| MS.gene05897 | MS.gene65127 | 0.831686 | 1.45E-55 | -1.69E-46 |
| MS.gene059440 | MS.gene65127 | 0.803441 | 3.48E-49 | -1.69E-46 |
| MS.gene059952 | MS.gene65127 | 0.822532 | 2.25E-53 | -1.69E-46 |
| MS.gene06052 | MS.gene65127 | 0.821328 | 4.28E-53 | -1.69E-46 |
| MS.gene060850 | MS.gene65127 | 0.80307 | 4.15E-49 | -1.69E-46 |
| MS.gene060852 | MS.gene65127 | 0.803204 | 3.90E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65127.t1 | MTR_7g059110 | 98.728 | 393 | 4 | 1 | 1 | 392 | 1 | 393 | 0.0 | 795 |
| MS.gene65127.t1 | MTR_7g059110 | 89.567 | 393 | 3 | 2 | 1 | 392 | 1 | 356 | 0.0 | 656 |
| MS.gene65127.t1 | MTR_4g113730 | 38.545 | 275 | 122 | 7 | 19 | 291 | 43 | 272 | 5.43e-58 | 193 |
| MS.gene65127.t1 | MTR_3g073240 | 61.165 | 103 | 40 | 0 | 180 | 282 | 128 | 230 | 7.49e-38 | 139 |
| MS.gene65127.t1 | MTR_3g073240 | 46.591 | 88 | 41 | 2 | 24 | 111 | 48 | 129 | 3.73e-16 | 79.0 |
| MS.gene65127.t1 | MTR_4g094420 | 46.528 | 144 | 72 | 2 | 141 | 279 | 90 | 233 | 4.17e-36 | 135 |
| MS.gene65127.t1 | MTR_4g094420 | 70.000 | 50 | 15 | 0 | 19 | 68 | 46 | 95 | 6.97e-19 | 87.8 |
| MS.gene65127.t1 | MTR_1g009790 | 46.479 | 142 | 71 | 2 | 174 | 315 | 94 | 230 | 3.81e-33 | 126 |
| MS.gene65127.t1 | MTR_1g009790 | 62.069 | 58 | 22 | 0 | 10 | 67 | 12 | 69 | 3.06e-16 | 79.3 |
| MS.gene65127.t1 | MTR_1g009790 | 46.479 | 142 | 71 | 2 | 174 | 315 | 94 | 230 | 4.89e-33 | 126 |
| MS.gene65127.t1 | MTR_1g009790 | 62.069 | 58 | 22 | 0 | 10 | 67 | 12 | 69 | 4.75e-16 | 79.0 |
| MS.gene65127.t1 | MTR_8g085870 | 51.724 | 87 | 42 | 0 | 180 | 266 | 134 | 220 | 4.91e-25 | 106 |
| MS.gene65127.t1 | MTR_8g085870 | 52.113 | 71 | 34 | 0 | 19 | 89 | 41 | 111 | 1.46e-17 | 84.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene65127.t1 | AT3G24740 | 51.058 | 378 | 124 | 9 | 1 | 367 | 1 | 328 | 5.55e-113 | 334 |
| MS.gene65127.t1 | AT3G24740 | 51.058 | 378 | 124 | 9 | 1 | 367 | 1 | 328 | 5.55e-113 | 334 |
| MS.gene65127.t1 | AT3G24740 | 51.058 | 378 | 124 | 9 | 1 | 367 | 1 | 328 | 5.55e-113 | 334 |
| MS.gene65127.t1 | AT3G24740 | 51.058 | 378 | 124 | 9 | 1 | 367 | 1 | 328 | 5.55e-113 | 334 |
| MS.gene65127.t1 | AT3G24740 | 51.058 | 378 | 124 | 9 | 1 | 367 | 1 | 328 | 5.55e-113 | 334 |
| MS.gene65127.t1 | AT3G24740 | 51.058 | 378 | 124 | 9 | 1 | 367 | 1 | 328 | 5.55e-113 | 334 |
| MS.gene65127.t1 | AT3G25910 | 37.984 | 258 | 135 | 6 | 19 | 275 | 36 | 269 | 2.57e-50 | 173 |
| MS.gene65127.t1 | AT4G31410 | 48.148 | 135 | 65 | 1 | 182 | 316 | 102 | 231 | 4.61e-35 | 131 |
| MS.gene65127.t1 | AT4G31410 | 55.357 | 56 | 23 | 1 | 22 | 77 | 30 | 83 | 1.89e-14 | 73.9 |
| MS.gene65127.t1 | AT4G31410 | 48.148 | 135 | 65 | 1 | 182 | 316 | 102 | 231 | 4.61e-35 | 131 |
| MS.gene65127.t1 | AT4G31410 | 55.357 | 56 | 23 | 1 | 22 | 77 | 30 | 83 | 1.89e-14 | 73.9 |
| MS.gene65127.t1 | AT4G31410 | 48.148 | 135 | 65 | 1 | 182 | 316 | 102 | 231 | 4.61e-35 | 131 |
| MS.gene65127.t1 | AT4G31410 | 55.357 | 56 | 23 | 1 | 22 | 77 | 30 | 83 | 1.89e-14 | 73.9 |
| MS.gene65127.t1 | AT4G08460 | 48.624 | 109 | 56 | 0 | 175 | 283 | 100 | 208 | 8.53e-33 | 124 |
| MS.gene65127.t1 | AT4G08460 | 51.786 | 56 | 25 | 1 | 24 | 77 | 47 | 102 | 5.09e-14 | 72.4 |
| MS.gene65127.t1 | AT4G08460 | 48.624 | 109 | 56 | 0 | 175 | 283 | 100 | 208 | 8.53e-33 | 124 |
| MS.gene65127.t1 | AT4G08460 | 51.786 | 56 | 25 | 1 | 24 | 77 | 47 | 102 | 5.09e-14 | 72.4 |
| MS.gene65127.t1 | AT4G08460 | 48.624 | 109 | 56 | 0 | 175 | 283 | 100 | 208 | 8.53e-33 | 124 |
| MS.gene65127.t1 | AT4G08460 | 51.786 | 56 | 25 | 1 | 24 | 77 | 47 | 102 | 5.09e-14 | 72.4 |
| MS.gene65127.t1 | AT4G08460 | 48.624 | 109 | 56 | 0 | 175 | 283 | 100 | 208 | 8.53e-33 | 124 |
| MS.gene65127.t1 | AT4G08460 | 51.786 | 56 | 25 | 1 | 24 | 77 | 47 | 102 | 5.09e-14 | 72.4 |
| MS.gene65127.t1 | AT1G68140 | 47.115 | 104 | 55 | 0 | 180 | 283 | 118 | 221 | 8.15e-30 | 117 |
| MS.gene65127.t1 | AT1G68140 | 68.000 | 50 | 16 | 0 | 19 | 68 | 48 | 97 | 1.29e-17 | 83.2 |
| MS.gene65127.t1 | AT1G68140 | 47.115 | 104 | 55 | 0 | 180 | 283 | 118 | 221 | 1.28e-29 | 117 |
| MS.gene65127.t1 | AT1G68140 | 68.000 | 50 | 16 | 0 | 19 | 68 | 48 | 97 | 1.44e-17 | 83.6 |
| MS.gene65127.t1 | AT1G68140 | 47.115 | 104 | 55 | 0 | 180 | 283 | 118 | 221 | 1.28e-29 | 117 |
| MS.gene65127.t1 | AT1G68140 | 68.000 | 50 | 16 | 0 | 19 | 68 | 48 | 97 | 1.44e-17 | 83.6 |
| MS.gene65127.t1 | AT1G68140 | 47.115 | 104 | 55 | 0 | 180 | 283 | 118 | 221 | 1.28e-29 | 117 |
| MS.gene65127.t1 | AT1G68140 | 68.000 | 50 | 16 | 0 | 19 | 68 | 48 | 97 | 1.44e-17 | 83.6 |
| MS.gene65127.t1 | AT1G77770 | 50.000 | 100 | 50 | 0 | 180 | 279 | 85 | 184 | 1.29e-27 | 110 |
| MS.gene65127.t1 | AT1G77770 | 50.000 | 66 | 32 | 1 | 19 | 83 | 24 | 89 | 2.65e-14 | 72.8 |
| MS.gene65127.t1 | AT1G77770 | 50.000 | 100 | 50 | 0 | 180 | 279 | 85 | 184 | 1.30e-27 | 110 |
| MS.gene65127.t1 | AT1G77770 | 50.000 | 66 | 32 | 1 | 19 | 83 | 24 | 89 | 2.99e-14 | 72.8 |
| MS.gene65127.t1 | AT1G77770 | 50.000 | 100 | 50 | 0 | 180 | 279 | 85 | 184 | 1.30e-27 | 110 |
| MS.gene65127.t1 | AT1G77770 | 50.000 | 66 | 32 | 1 | 19 | 83 | 24 | 89 | 2.99e-14 | 72.8 |
| MS.gene65127.t1 | AT2G26050 | 42.353 | 85 | 48 | 1 | 179 | 262 | 71 | 155 | 9.16e-17 | 79.0 |
| MS.gene65127.t1 | AT1G15430 | 39.535 | 86 | 52 | 0 | 179 | 264 | 106 | 191 | 1.09e-16 | 79.7 |
| MS.gene65127.t1 | AT1G15430 | 63.830 | 47 | 17 | 0 | 20 | 66 | 47 | 93 | 6.21e-14 | 71.6 |
| MS.gene65127.t1 | AT1G15430 | 39.535 | 86 | 52 | 0 | 179 | 264 | 106 | 191 | 1.09e-16 | 79.7 |
| MS.gene65127.t1 | AT1G15430 | 63.830 | 47 | 17 | 0 | 20 | 66 | 47 | 93 | 6.21e-14 | 71.6 |
| MS.gene65127.t1 | AT1G80220 | 39.806 | 103 | 59 | 2 | 179 | 280 | 100 | 200 | 2.22e-16 | 78.6 |
| MS.gene65127.t1 | AT1G80220 | 53.571 | 56 | 22 | 1 | 22 | 73 | 43 | 98 | 9.22e-12 | 65.1 |
Find 105 sgRNAs with CRISPR-Local
Find 104 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTAAAGAACTTTGTAAATT+TGG | 0.086986 | 7.1:-42434398 | None:intergenic |
| CTCATTTGCTGGAGATTATC+TGG | 0.236537 | 7.1:+42434811 | MS.gene65127:CDS |
| CTGACCGCCGTCGCTATCTT+TGG | 0.243082 | 7.1:+42435185 | MS.gene65127:CDS |
| GCAGGACAGGGCTGTTCTTC+AGG | 0.263206 | 7.1:+42434640 | MS.gene65127:CDS |
| GGGGATTACGTTCTCGAAAA+TGG | 0.268380 | 7.1:+42434989 | MS.gene65127:CDS |
| ACATTTGTGATACAAGTTAT+AGG | 0.274353 | 7.1:+42434321 | MS.gene65127:CDS |
| TTAAAGAACTTTGTAAATTT+GGG | 0.285619 | 7.1:-42434397 | None:intergenic |
| ATAGAGTATTAATTTCATTT+TGG | 0.288715 | 7.1:-42434508 | None:intergenic |
| CTATGCAGGGGCACAGTATT+AGG | 0.290784 | 7.1:+42434719 | MS.gene65127:CDS |
| CAATGGTGTTATCCATCATT+TGG | 0.297444 | 7.1:-42435102 | None:intergenic |
| TGACCGCCGTCGCTATCTTT+GGG | 0.311516 | 7.1:+42435186 | MS.gene65127:CDS |
| CAATATCACCATATTCTCTT+TGG | 0.322903 | 7.1:-42434925 | None:intergenic |
| TGCCACGCTTGTTCTCTTGT+CGG | 0.342123 | 7.1:-42434890 | None:intergenic |
| AGGGATTCATGCTCATTTGC+TGG | 0.342371 | 7.1:+42434800 | MS.gene65127:CDS |
| TACCAGGAGCTGTGGTAGTT+GGG | 0.361459 | 7.1:+42434969 | MS.gene65127:CDS |
| CTAAATGTCTACTTGGATCT+TGG | 0.362438 | 7.1:-42434571 | None:intergenic |
| GTTTGCATTGTCCTCTATGC+AGG | 0.369372 | 7.1:+42434705 | MS.gene65127:CDS |
| TACCAGGTGGTGGTAGGGAG+GGG | 0.376072 | 7.1:+42435029 | MS.gene65127:CDS |
| CTTTGGGGTGAGAATTTGTT+GGG | 0.387062 | 7.1:+42435202 | MS.gene65127:CDS |
| TTTGAGCGCCAAAGAGAATA+TGG | 0.388978 | 7.1:+42434917 | MS.gene65127:CDS |
| TCTACGAATATTTAATGAAC+TGG | 0.389467 | 7.1:+42435252 | MS.gene65127:CDS |
| GTTAATATCAAAACTATTAC+CGG | 0.394163 | 7.1:-42434438 | None:intergenic |
| GCATGCTCTTCACAGAGAAT+TGG | 0.394274 | 7.1:+42434211 | MS.gene65127:CDS |
| TTCAAACGACGGCGCCTCCT+TGG | 0.409832 | 7.1:-42435295 | None:intergenic |
| TTATAGGCATTCAAATTGTC+TGG | 0.411863 | 7.1:+42434337 | MS.gene65127:CDS |
| TGCTGATTCTGAGAACTTGC+AGG | 0.412339 | 7.1:+42434622 | MS.gene65127:CDS |
| ATAAACACTAACAACTCTTC+CGG | 0.422488 | 7.1:+42434419 | MS.gene65127:CDS |
| ACTTTGCAGCTCTCATGATA+AGG | 0.425611 | 7.1:+42434286 | MS.gene65127:CDS |
| ATTAATACTCTATTGTCTGT+TGG | 0.425972 | 7.1:+42434518 | MS.gene65127:CDS |
| TCTTTGGGGTGAGAATTTGT+TGG | 0.432599 | 7.1:+42435201 | MS.gene65127:CDS |
| ATTCTGAGAACTTGCAGGAC+AGG | 0.434425 | 7.1:+42434627 | MS.gene65127:CDS |
| TAAATGTCTACTTGGATCTT+GGG | 0.439889 | 7.1:-42434570 | None:intergenic |
| TGGTTCTCTAACAATTTCAA+TGG | 0.446626 | 7.1:-42435119 | None:intergenic |
| CACCATCTTGTTCCAAATGA+TGG | 0.449976 | 7.1:+42435090 | MS.gene65127:CDS |
| TCAAACGACGGCGCCTCCTT+GGG | 0.458119 | 7.1:-42435294 | None:intergenic |
| CTTTGCAGCTCTCATGATAA+GGG | 0.464712 | 7.1:+42434287 | MS.gene65127:CDS |
| GGAGGCGCCGTCGTTTGAAT+AGG | 0.466130 | 7.1:+42435299 | MS.gene65127:CDS |
| CCATTCGATGAACGAGCTCT+TGG | 0.466363 | 7.1:-42435139 | None:intergenic |
| GTTACCAGGTGGTGGTAGGG+AGG | 0.472492 | 7.1:+42435027 | MS.gene65127:CDS |
| CTCGAAAATGGAGATGGTAT+TGG | 0.491652 | 7.1:+42435001 | MS.gene65127:CDS |
| TTGGAACAAGATGGTGGTAG+TGG | 0.494227 | 7.1:-42435083 | None:intergenic |
| GATGGTATTGGTAGGTTACC+AGG | 0.501983 | 7.1:+42435013 | MS.gene65127:CDS |
| TGTGGATGGTCCATGCAGAT+AGG | 0.502449 | 7.1:-42434248 | None:intergenic |
| AATTCCACAAGGATCGAGAC+AGG | 0.502538 | 7.1:+42434541 | MS.gene65127:CDS |
| GTAGGTTACCAGGTGGTGGT+AGG | 0.505182 | 7.1:+42435023 | MS.gene65127:CDS |
| ATACCAGGAGCTGTGGTAGT+TGG | 0.506423 | 7.1:+42434968 | MS.gene65127:CDS |
| GCAGGGGCACAGTATTAGGC+TGG | 0.506826 | 7.1:+42434723 | MS.gene65127:CDS |
| TGGAATTGCGTAGGCATGCT+AGG | 0.511583 | 7.1:+42434831 | MS.gene65127:CDS |
| TAGGTTACCAGGTGGTGGTA+GGG | 0.512795 | 7.1:+42435024 | MS.gene65127:CDS |
| GCTATTCAGTCTGCCATACC+AGG | 0.516031 | 7.1:+42434953 | MS.gene65127:CDS |
| TGTTGGTCTAAATGTCTACT+TGG | 0.518204 | 7.1:-42434578 | None:intergenic |
| CAAACTCGACTTTGAGTCCT+CGG | 0.522532 | 7.1:-42434687 | None:intergenic |
| GACCGCCGTCGCTATCTTTG+GGG | 0.523480 | 7.1:+42435187 | MS.gene65127:CDS |
| GGTGGTAGGGAGGGGAGCAA+TGG | 0.523498 | 7.1:+42435037 | MS.gene65127:CDS |
| CTAAAATGCCTTCATCATGT+TGG | 0.523887 | 7.1:-42434595 | None:intergenic |
| ACATCAGAGGGACGAGAAGT+AGG | 0.527291 | 7.1:-42434863 | None:intergenic |
| TATCATGAGAGCTGCAAAGT+AGG | 0.528215 | 7.1:-42434283 | None:intergenic |
| TTTGCATTGTCCTCTATGCA+GGG | 0.529859 | 7.1:+42434706 | MS.gene65127:CDS |
| CAATCATGAATGATCTTCGT+TGG | 0.532620 | 7.1:-42435329 | None:intergenic |
| TTCTGAGAACTTGCAGGACA+GGG | 0.533978 | 7.1:+42434628 | MS.gene65127:CDS |
| ATTGGTAGGTTACCAGGTGG+TGG | 0.534392 | 7.1:+42435019 | MS.gene65127:CDS |
| TTACCAGGTGGTGGTAGGGA+GGG | 0.536579 | 7.1:+42435028 | MS.gene65127:CDS |
| CTCCCCTCCCTACCACCACC+TGG | 0.537599 | 7.1:-42435031 | None:intergenic |
| ATCCATCATTTGGAACAAGA+TGG | 0.540246 | 7.1:-42435092 | None:intergenic |
| AACTACCACAGCTCCTGGTA+TGG | 0.541534 | 7.1:-42434966 | None:intergenic |
| TCCCCAACTACCACAGCTCC+TGG | 0.541696 | 7.1:-42434971 | None:intergenic |
| ACCAGGAGCTGTGGTAGTTG+GGG | 0.553461 | 7.1:+42434970 | MS.gene65127:CDS |
| CCAAGAGCTCGTTCATCGAA+TGG | 0.555087 | 7.1:+42435139 | MS.gene65127:CDS |
| CACGGTGCGAGGAAGATGGC+TGG | 0.561637 | 7.1:+42434155 | None:intergenic |
| CATTTAGACCAACATGATGA+AGG | 0.566826 | 7.1:+42434587 | MS.gene65127:CDS |
| AATACTGTGCCCCTGCATAG+AGG | 0.571635 | 7.1:-42434716 | None:intergenic |
| AGTAGGAGAACAGCATTATG+TGG | 0.571923 | 7.1:-42434266 | None:intergenic |
| AGGGAGGGGAGCAATGGCAA+TGG | 0.578428 | 7.1:+42435043 | MS.gene65127:CDS |
| GAGCTCGTTCATCGAATGGA+TGG | 0.583468 | 7.1:+42435143 | MS.gene65127:CDS |
| TCACCCTGTCTCGATCCTTG+TGG | 0.586932 | 7.1:-42434545 | None:intergenic |
| GTCTGCCATACCAGGAGCTG+TGG | 0.587043 | 7.1:+42434961 | MS.gene65127:CDS |
| ATAAGAAGAGAAGTTGCTCA+AGG | 0.588030 | 7.1:+42434780 | MS.gene65127:CDS |
| GGAGAACAGCATTATGTGGA+TGG | 0.588463 | 7.1:-42434262 | None:intergenic |
| TTGCATTGTCCTCTATGCAG+GGG | 0.590896 | 7.1:+42434707 | MS.gene65127:CDS |
| TACGTTCTCGAAAATGGAGA+TGG | 0.597289 | 7.1:+42434995 | MS.gene65127:CDS |
| GAGATTATCTGGAATTGCGT+AGG | 0.608619 | 7.1:+42434822 | MS.gene65127:CDS |
| ATGGCAATGGTAATGTACCT+TGG | 0.614535 | 7.1:+42435056 | MS.gene65127:CDS |
| AGTTTCATGTCCTATCTGCA+TGG | 0.615030 | 7.1:+42434238 | MS.gene65127:CDS |
| TAAGAAGAGAAGTTGCTCAA+GGG | 0.617758 | 7.1:+42434781 | MS.gene65127:CDS |
| GTTCATCGAATGGATGGTCA+AGG | 0.630634 | 7.1:+42435149 | MS.gene65127:CDS |
| AAAATGGAGATGGTATTGGT+AGG | 0.633136 | 7.1:+42435005 | MS.gene65127:CDS |
| AAAATTGAGAGACAACTCTA+CGG | 0.637192 | 7.1:+42434370 | MS.gene65127:CDS |
| AAGATGCATCTCATGTCCCA+AGG | 0.639920 | 7.1:+42435278 | MS.gene65127:CDS |
| CAGGGGCACAGTATTAGGCT+GGG | 0.647019 | 7.1:+42434724 | MS.gene65127:CDS |
| GGACAGGGCTGTTCTTCAGG+AGG | 0.650346 | 7.1:+42434643 | MS.gene65127:CDS |
| TCTTGTCGGATCAACATCAG+AGG | 0.652568 | 7.1:-42434876 | None:intergenic |
| CATCATTTGGAACAAGATGG+TGG | 0.659005 | 7.1:-42435089 | None:intergenic |
| ACCTCGTCCTATTCAAACGA+CGG | 0.662069 | 7.1:-42435306 | None:intergenic |
| TTGTCTGTTGGAATTCCACA+AGG | 0.662938 | 7.1:+42434530 | MS.gene65127:CDS |
| ATTCCACAAGGATCGAGACA+GGG | 0.664648 | 7.1:+42434542 | MS.gene65127:CDS |
| CTTGTCGGATCAACATCAGA+GGG | 0.668547 | 7.1:-42434875 | None:intergenic |
| ATCCGACAAGAGAACAAGCG+TGG | 0.676885 | 7.1:+42434888 | MS.gene65127:CDS |
| AGATGTAGATAATTCATCCG+AGG | 0.680649 | 7.1:+42434670 | MS.gene65127:CDS |
| CACCCCAAAGATAGCGACGG+CGG | 0.684204 | 7.1:-42435189 | None:intergenic |
| TCTCACCCCAAAGATAGCGA+CGG | 0.686564 | 7.1:-42435192 | None:intergenic |
| GCCGTCGTTTGAATAGGACG+AGG | 0.693738 | 7.1:+42435305 | MS.gene65127:CDS |
| GGGCACAGTATTAGGCTGGG+AGG | 0.718146 | 7.1:+42434727 | MS.gene65127:CDS |
| ATGCATCTCATGTCCCAAGG+AGG | 0.718218 | 7.1:+42435281 | MS.gene65127:CDS |
| ATGGTGGTAGTGGTTAACCA+AGG | 0.736997 | 7.1:-42435073 | None:intergenic |
| GGTATTGGTAGGTTACCAGG+TGG | 0.762448 | 7.1:+42435016 | MS.gene65127:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTAAAGAACTTTGTAAATT+TGG | - | chr7.1:42434401-42434420 | None:intergenic | 15.0% |
| !! | TTAAAGAACTTTGTAAATTT+GGG | - | chr7.1:42434400-42434419 | None:intergenic | 15.0% |
| !!! | ATAGAGTATTAATTTCATTT+TGG | - | chr7.1:42434511-42434530 | None:intergenic | 15.0% |
| !! | GTTAATATCAAAACTATTAC+CGG | - | chr7.1:42434441-42434460 | None:intergenic | 20.0% |
| !!! | TGTCTCTCAATTTTTTAAAT+CGG | - | chr7.1:42434364-42434383 | None:intergenic | 20.0% |
| ! | ACATTTGTGATACAAGTTAT+AGG | + | chr7.1:42434321-42434340 | MS.gene65127:CDS | 25.0% |
| ! | ATTAATACTCTATTGTCTGT+TGG | + | chr7.1:42434518-42434537 | MS.gene65127:CDS | 25.0% |
| ! | TCTACGAATATTTAATGAAC+TGG | + | chr7.1:42435252-42435271 | MS.gene65127:CDS | 25.0% |
| AAAATTGAGAGACAACTCTA+CGG | + | chr7.1:42434370-42434389 | MS.gene65127:CDS | 30.0% | |
| ATAAACACTAACAACTCTTC+CGG | + | chr7.1:42434419-42434438 | MS.gene65127:CDS | 30.0% | |
| CAATATCACCATATTCTCTT+TGG | - | chr7.1:42434928-42434947 | None:intergenic | 30.0% | |
| TAAATGTCTACTTGGATCTT+GGG | - | chr7.1:42434573-42434592 | None:intergenic | 30.0% | |
| TGGTTCTCTAACAATTTCAA+TGG | - | chr7.1:42435122-42435141 | None:intergenic | 30.0% | |
| TTATAGGCATTCAAATTGTC+TGG | + | chr7.1:42434337-42434356 | MS.gene65127:CDS | 30.0% | |
| AGATGTAGATAATTCATCCG+AGG | + | chr7.1:42434670-42434689 | MS.gene65127:CDS | 35.0% | |
| ATAAGAAGAGAAGTTGCTCA+AGG | + | chr7.1:42434780-42434799 | MS.gene65127:CDS | 35.0% | |
| CATTTAGACCAACATGATGA+AGG | + | chr7.1:42434587-42434606 | MS.gene65127:CDS | 35.0% | |
| CTAAAATGCCTTCATCATGT+TGG | - | chr7.1:42434598-42434617 | None:intergenic | 35.0% | |
| CTAAATGTCTACTTGGATCT+TGG | - | chr7.1:42434574-42434593 | None:intergenic | 35.0% | |
| TAAGAAGAGAAGTTGCTCAA+GGG | + | chr7.1:42434781-42434800 | MS.gene65127:CDS | 35.0% | |
| ! | ATCCATCATTTGGAACAAGA+TGG | - | chr7.1:42435095-42435114 | None:intergenic | 35.0% |
| ! | TGTTGGTCTAAATGTCTACT+TGG | - | chr7.1:42434581-42434600 | None:intergenic | 35.0% |
| !! | AAAATGGAGATGGTATTGGT+AGG | + | chr7.1:42435005-42435024 | MS.gene65127:CDS | 35.0% |
| !! | CAATGGTGTTATCCATCATT+TGG | - | chr7.1:42435105-42435124 | None:intergenic | 35.0% |
| ACTTTGCAGCTCTCATGATA+AGG | + | chr7.1:42434286-42434305 | MS.gene65127:CDS | 40.0% | |
| AGTAGGAGAACAGCATTATG+TGG | - | chr7.1:42434269-42434288 | None:intergenic | 40.0% | |
| AGTTTCATGTCCTATCTGCA+TGG | + | chr7.1:42434238-42434257 | MS.gene65127:CDS | 40.0% | |
| CACCATCTTGTTCCAAATGA+TGG | + | chr7.1:42435090-42435109 | MS.gene65127:CDS | 40.0% | |
| CTCGAAAATGGAGATGGTAT+TGG | + | chr7.1:42435001-42435020 | MS.gene65127:CDS | 40.0% | |
| CTTTGCAGCTCTCATGATAA+GGG | + | chr7.1:42434287-42434306 | MS.gene65127:CDS | 40.0% | |
| TACGTTCTCGAAAATGGAGA+TGG | + | chr7.1:42434995-42435014 | MS.gene65127:CDS | 40.0% | |
| TATCATGAGAGCTGCAAAGT+AGG | - | chr7.1:42434286-42434305 | None:intergenic | 40.0% | |
| TTGTCTGTTGGAATTCCACA+AGG | + | chr7.1:42434530-42434549 | MS.gene65127:CDS | 40.0% | |
| TTTGAGCGCCAAAGAGAATA+TGG | + | chr7.1:42434917-42434936 | MS.gene65127:CDS | 40.0% | |
| TTTGCATTGTCCTCTATGCA+GGG | + | chr7.1:42434706-42434725 | MS.gene65127:CDS | 40.0% | |
| ! | ATGGCAATGGTAATGTACCT+TGG | + | chr7.1:42435056-42435075 | MS.gene65127:CDS | 40.0% |
| ! | CATCATTTGGAACAAGATGG+TGG | - | chr7.1:42435092-42435111 | None:intergenic | 40.0% |
| ! | CTCATTTGCTGGAGATTATC+TGG | + | chr7.1:42434811-42434830 | MS.gene65127:CDS | 40.0% |
| ! | GAGATTATCTGGAATTGCGT+AGG | + | chr7.1:42434822-42434841 | MS.gene65127:CDS | 40.0% |
| !! | CTTTGGGGTGAGAATTTGTT+GGG | + | chr7.1:42435202-42435221 | MS.gene65127:CDS | 40.0% |
| !! | TCTTTGGGGTGAGAATTTGT+TGG | + | chr7.1:42435201-42435220 | MS.gene65127:CDS | 40.0% |
| AAGATGCATCTCATGTCCCA+AGG | + | chr7.1:42435278-42435297 | MS.gene65127:CDS | 45.0% | |
| AATTCCACAAGGATCGAGAC+AGG | + | chr7.1:42434541-42434560 | MS.gene65127:CDS | 45.0% | |
| ACCTCGTCCTATTCAAACGA+CGG | - | chr7.1:42435309-42435328 | None:intergenic | 45.0% | |
| AGGGATTCATGCTCATTTGC+TGG | + | chr7.1:42434800-42434819 | MS.gene65127:CDS | 45.0% | |
| ATTCCACAAGGATCGAGACA+GGG | + | chr7.1:42434542-42434561 | MS.gene65127:CDS | 45.0% | |
| CAAACTCGACTTTGAGTCCT+CGG | - | chr7.1:42434690-42434709 | None:intergenic | 45.0% | |
| CTTGTCGGATCAACATCAGA+GGG | - | chr7.1:42434878-42434897 | None:intergenic | 45.0% | |
| GCATGCTCTTCACAGAGAAT+TGG | + | chr7.1:42434211-42434230 | MS.gene65127:CDS | 45.0% | |
| GGAGAACAGCATTATGTGGA+TGG | - | chr7.1:42434265-42434284 | None:intergenic | 45.0% | |
| GGGGATTACGTTCTCGAAAA+TGG | + | chr7.1:42434989-42435008 | MS.gene65127:CDS | 45.0% | |
| GTTCATCGAATGGATGGTCA+AGG | + | chr7.1:42435149-42435168 | MS.gene65127:CDS | 45.0% | |
| GTTTGCATTGTCCTCTATGC+AGG | + | chr7.1:42434705-42434724 | MS.gene65127:CDS | 45.0% | |
| TCTTGTCGGATCAACATCAG+AGG | - | chr7.1:42434879-42434898 | None:intergenic | 45.0% | |
| TTGCATTGTCCTCTATGCAG+GGG | + | chr7.1:42434707-42434726 | MS.gene65127:CDS | 45.0% | |
| ! | ATTCTGAGAACTTGCAGGAC+AGG | + | chr7.1:42434627-42434646 | MS.gene65127:CDS | 45.0% |
| ! | TTCTGAGAACTTGCAGGACA+GGG | + | chr7.1:42434628-42434647 | MS.gene65127:CDS | 45.0% |
| ! | TTGGAACAAGATGGTGGTAG+TGG | - | chr7.1:42435086-42435105 | None:intergenic | 45.0% |
| !! | ATGGTGGTAGTGGTTAACCA+AGG | - | chr7.1:42435076-42435095 | None:intergenic | 45.0% |
| !! | GATGGTATTGGTAGGTTACC+AGG | + | chr7.1:42435013-42435032 | MS.gene65127:CDS | 45.0% |
| !! | TGCTGATTCTGAGAACTTGC+AGG | + | chr7.1:42434622-42434641 | MS.gene65127:CDS | 45.0% |
| AACTACCACAGCTCCTGGTA+TGG | - | chr7.1:42434969-42434988 | None:intergenic | 50.0% | |
| AATACTGTGCCCCTGCATAG+AGG | - | chr7.1:42434719-42434738 | None:intergenic | 50.0% | |
| ACATCAGAGGGACGAGAAGT+AGG | - | chr7.1:42434866-42434885 | None:intergenic | 50.0% | |
| ATACCAGGAGCTGTGGTAGT+TGG | + | chr7.1:42434968-42434987 | MS.gene65127:CDS | 50.0% | |
| ATCCGACAAGAGAACAAGCG+TGG | + | chr7.1:42434888-42434907 | MS.gene65127:CDS | 50.0% | |
| ATGCATCTCATGTCCCAAGG+AGG | + | chr7.1:42435281-42435300 | MS.gene65127:CDS | 50.0% | |
| ATTGGTAGGTTACCAGGTGG+TGG | + | chr7.1:42435019-42435038 | MS.gene65127:CDS | 50.0% | |
| CCAAGAGCTCGTTCATCGAA+TGG | + | chr7.1:42435139-42435158 | MS.gene65127:CDS | 50.0% | |
| CCATTCGATGAACGAGCTCT+TGG | - | chr7.1:42435142-42435161 | None:intergenic | 50.0% | |
| GAGCTCGTTCATCGAATGGA+TGG | + | chr7.1:42435143-42435162 | MS.gene65127:CDS | 50.0% | |
| GCTATTCAGTCTGCCATACC+AGG | + | chr7.1:42434953-42434972 | MS.gene65127:CDS | 50.0% | |
| TACCAGGAGCTGTGGTAGTT+GGG | + | chr7.1:42434969-42434988 | MS.gene65127:CDS | 50.0% | |
| TCTCACCCCAAAGATAGCGA+CGG | - | chr7.1:42435195-42435214 | None:intergenic | 50.0% | |
| TGCCACGCTTGTTCTCTTGT+CGG | - | chr7.1:42434893-42434912 | None:intergenic | 50.0% | |
| TGGAATTGCGTAGGCATGCT+AGG | + | chr7.1:42434831-42434850 | MS.gene65127:CDS | 50.0% | |
| TGTGGATGGTCCATGCAGAT+AGG | - | chr7.1:42434251-42434270 | None:intergenic | 50.0% | |
| ! | CTATGCAGGGGCACAGTATT+AGG | + | chr7.1:42434719-42434738 | MS.gene65127:CDS | 50.0% |
| ! | TAGGTTACCAGGTGGTGGTA+GGG | + | chr7.1:42435024-42435043 | MS.gene65127:CDS | 50.0% |
| !! | GGTATTGGTAGGTTACCAGG+TGG | + | chr7.1:42435016-42435035 | MS.gene65127:CDS | 50.0% |
| ACCAGGAGCTGTGGTAGTTG+GGG | + | chr7.1:42434970-42434989 | MS.gene65127:CDS | 55.0% | |
| GCCGTCGTTTGAATAGGACG+AGG | + | chr7.1:42435305-42435324 | MS.gene65127:CDS | 55.0% | |
| TCACCCTGTCTCGATCCTTG+TGG | - | chr7.1:42434548-42434567 | None:intergenic | 55.0% | |
| TGACCGCCGTCGCTATCTTT+GGG | + | chr7.1:42435186-42435205 | MS.gene65127:CDS | 55.0% | |
| ! | CAGGGGCACAGTATTAGGCT+GGG | + | chr7.1:42434724-42434743 | MS.gene65127:CDS | 55.0% |
| ! | GTAGGTTACCAGGTGGTGGT+AGG | + | chr7.1:42435023-42435042 | MS.gene65127:CDS | 55.0% |
| ! | TTACCAGGTGGTGGTAGGGA+GGG | + | chr7.1:42435028-42435047 | MS.gene65127:CDS | 55.0% |
| AGGGAGGGGAGCAATGGCAA+TGG | + | chr7.1:42435043-42435062 | MS.gene65127:CDS | 60.0% | |
| CACCCCAAAGATAGCGACGG+CGG | - | chr7.1:42435192-42435211 | None:intergenic | 60.0% | |
| CTGACCGCCGTCGCTATCTT+TGG | + | chr7.1:42435185-42435204 | MS.gene65127:CDS | 60.0% | |
| GACCGCCGTCGCTATCTTTG+GGG | + | chr7.1:42435187-42435206 | MS.gene65127:CDS | 60.0% | |
| GCAGGACAGGGCTGTTCTTC+AGG | + | chr7.1:42434640-42434659 | MS.gene65127:CDS | 60.0% | |
| GGACAGGGCTGTTCTTCAGG+AGG | + | chr7.1:42434643-42434662 | MS.gene65127:CDS | 60.0% | |
| GGGCACAGTATTAGGCTGGG+AGG | + | chr7.1:42434727-42434746 | MS.gene65127:CDS | 60.0% | |
| GTCTGCCATACCAGGAGCTG+TGG | + | chr7.1:42434961-42434980 | MS.gene65127:CDS | 60.0% | |
| TCAAACGACGGCGCCTCCTT+GGG | - | chr7.1:42435297-42435316 | None:intergenic | 60.0% | |
| TCCCCAACTACCACAGCTCC+TGG | - | chr7.1:42434974-42434993 | None:intergenic | 60.0% | |
| TTCAAACGACGGCGCCTCCT+TGG | - | chr7.1:42435298-42435317 | None:intergenic | 60.0% | |
| ! | GCAGGGGCACAGTATTAGGC+TGG | + | chr7.1:42434723-42434742 | MS.gene65127:CDS | 60.0% |
| ! | GTTACCAGGTGGTGGTAGGG+AGG | + | chr7.1:42435027-42435046 | MS.gene65127:CDS | 60.0% |
| ! | TACCAGGTGGTGGTAGGGAG+GGG | + | chr7.1:42435029-42435048 | MS.gene65127:CDS | 60.0% |
| !! | GGAGGCGCCGTCGTTTGAAT+AGG | + | chr7.1:42435299-42435318 | MS.gene65127:CDS | 60.0% |
| GGTGGTAGGGAGGGGAGCAA+TGG | + | chr7.1:42435037-42435056 | MS.gene65127:CDS | 65.0% | |
| CTCCCCTCCCTACCACCACC+TGG | - | chr7.1:42435034-42435053 | None:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 42434170 | 42435348 | 42434170 | ID=MS.gene65127 |
| chr7.1 | mRNA | 42434170 | 42435348 | 42434170 | ID=MS.gene65127.t1;Parent=MS.gene65127 |
| chr7.1 | exon | 42434170 | 42435348 | 42434170 | ID=MS.gene65127.t1.exon1;Parent=MS.gene65127.t1 |
| chr7.1 | CDS | 42434170 | 42435348 | 42434170 | ID=cds.MS.gene65127.t1;Parent=MS.gene65127.t1 |
| Gene Sequence |
| Protein sequence |
