AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene65682


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65682.t1 MTR_5g056480 92.453 318 20 1 1 318 1 314 0.0 541
MS.gene65682.t1 MTR_1g110550 40.484 289 112 9 1 280 4 241 6.55e-52 171
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene65682.t1 AT1G68440 33.129 326 179 9 1 309 1 304 1.62e-36 133
MS.gene65682.t1 AT1G25400 36.486 148 78 5 155 299 144 278 2.56e-14 72.4
MS.gene65682.t1 AT1G25400 36.486 148 78 5 155 299 144 278 2.56e-14 72.4

Find 75 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATCTTACGGGAAACATTAA+TGG 0.287710 5.2:+48464797 MS.gene65682:CDS
TCGTTGAGTCTATCGATTAA+TGG 0.309567 5.2:-48463869 None:intergenic
GTAATCTCGCAGTCGAGATA+TGG 0.314723 5.2:+48464542 MS.gene65682:CDS
ACGAAGGTGGACGATGAAAA+TGG 0.314900 5.2:-48464062 None:intergenic
CTTAACAACGTTATCACTAT+TGG 0.316917 5.2:-48464362 None:intergenic
GTGATAACGTTGTTAAGCTT+TGG 0.325270 5.2:+48464368 MS.gene65682:CDS
CTTTGGGACAGTATAGGATT+TGG 0.332824 5.2:+48464385 MS.gene65682:CDS
TTGCTTTCAATTTCTGCAAA+TGG 0.333771 5.2:+48463984 MS.gene65682:CDS
AACACGCTGCGCGTGGAATC+TGG 0.336904 5.2:+48464625 MS.gene65682:CDS
TGCTTTCAATTTCTGCAAAT+GGG 0.348348 5.2:+48463985 MS.gene65682:CDS
TGATAACGTTGTTAAGCTTT+GGG 0.361870 5.2:+48464369 MS.gene65682:CDS
GACAGTATAGGATTTGGTTT+AGG 0.399313 5.2:+48464391 MS.gene65682:CDS
TTGTTACGGATGAATCTTAC+GGG 0.415731 5.2:+48464785 MS.gene65682:CDS
CCTGCGGAGACGATCACATC+TGG 0.432622 5.2:-48464505 None:intergenic
CGAGTTACAGGAAACAGTTA+TGG 0.432779 5.2:+48464250 MS.gene65682:CDS
ATCACAACCACTTCCTTCAA+TGG 0.443517 5.2:+48463944 MS.gene65682:CDS
TGCTGATGCTGTTATTGTTA+CGG 0.444662 5.2:+48464771 MS.gene65682:CDS
GTTAAGCTTTGGGACAGTAT+AGG 0.445462 5.2:+48464379 MS.gene65682:CDS
AACGGATTGGATTCCATTGA+AGG 0.457478 5.2:-48463957 None:intergenic
CTTGATGAAGATGACATAGA+TGG 0.461410 5.2:-48464211 None:intergenic
TGAAAGCAATGGAAACGGAT+TGG 0.472954 5.2:-48463970 None:intergenic
TCGTTGTCGAAGGTGATTGA+TGG 0.476471 5.2:-48464103 None:intergenic
GTGATTTGTGAAGGGAAAGA+TGG 0.477961 5.2:-48463926 None:intergenic
TGATTTGTGAAGGGAAAGAT+GGG 0.488143 5.2:-48463925 None:intergenic
AATCTGACGCGGATAATACT+TGG 0.488206 5.2:+48464743 MS.gene65682:CDS
AGACTCAACGATTTCCAAGC+AGG 0.489396 5.2:+48463881 MS.gene65682:CDS
GATTTCGATGATTACGAGGA+TGG 0.489828 5.2:+48464418 MS.gene65682:CDS
TTGGATTCCATTGAAGGAAG+TGG 0.497956 5.2:-48463951 None:intergenic
TTTGCAGAAATTGAAAGCAA+TGG 0.502375 5.2:-48463981 None:intergenic
ATTGTTACGGATGAATCTTA+CGG 0.506144 5.2:+48464784 MS.gene65682:CDS
AAAGTTTATGTTAGAGATGA+CGG 0.508455 5.2:+48464658 MS.gene65682:CDS
GCCGTCGGTCGTAGCCGAGT+GGG 0.511064 5.2:+48464588 MS.gene65682:CDS
TAATCTCGCAGTCGAGATAT+GGG 0.515982 5.2:+48464543 MS.gene65682:CDS
CGTTAGTTTATCTTCGTCAT+CGG 0.519809 5.2:+48464171 MS.gene65682:CDS
TTCGTTATCTTCGTCATCGG+AGG 0.521784 5.2:-48464137 None:intergenic
CATAGACGACAACGTAGTAT+TGG 0.523774 5.2:+48464313 MS.gene65682:CDS
GGACGTCAACGTTTGACAGT+TGG 0.524989 5.2:+48464679 MS.gene65682:CDS
GATGCGGCCGCAGAAGTTGA+AGG 0.525538 5.2:-48464481 None:intergenic
GAAATCATCGTCGTTGTCGA+AGG 0.525729 5.2:-48464113 None:intergenic
GAAATTGAAAGCAATGGAAA+CGG 0.527809 5.2:-48463975 None:intergenic
AGAAAAGTCAGTGTTCCGTT+AGG 0.537364 5.2:+48464709 MS.gene65682:CDS
CGACCGACGGCTTCCGTCGT+CGG 0.539535 5.2:-48464576 None:intergenic
CCGTCGGTCGTAGCCGAGTG+GGG 0.540475 5.2:+48464589 MS.gene65682:CDS
GGTGACTTTGTTGATGATGC+TGG 0.548195 5.2:-48464041 None:intergenic
CCAGATGTGATCGTCTCCGC+AGG 0.548921 5.2:+48464505 MS.gene65682:CDS
GTGATCGTCTCCGCAGGAGA+AGG 0.560004 5.2:+48464511 MS.gene65682:CDS
GCTTTCAATTTCTGCAAATG+GGG 0.560368 5.2:+48463986 MS.gene65682:CDS
GGTGGGGAACACGCTGCGCG+TGG 0.563432 5.2:+48464618 MS.gene65682:CDS
GTCGAAGGTGATTGATGGAA+GGG 0.564506 5.2:-48464098 None:intergenic
GGGACACTCGTCTCCGACGA+CGG 0.568741 5.2:+48464563 MS.gene65682:CDS
GCAATGAATTGAAACCTGCT+TGG 0.577118 5.2:-48463895 None:intergenic
CCCCACTCGGCTACGACCGA+CGG 0.583274 5.2:-48464589 None:intergenic
CGCAGCGTGTTCCCCACCGT+GGG 0.586011 5.2:-48464613 None:intergenic
GAAGTGGTTGTGATTTGTGA+AGG 0.591968 5.2:-48463935 None:intergenic
TGTCGAAGGTGATTGATGGA+AGG 0.594870 5.2:-48464099 None:intergenic
GCAGAAGTTGAAGGCGCATG+TGG 0.595970 5.2:-48464472 None:intergenic
CTGACGCGGATAATACTTGG+TGG 0.603373 5.2:+48464746 MS.gene65682:CDS
AACGTGTTCGAAACTCAAAA+CGG 0.603538 5.2:+48464286 MS.gene65682:CDS
ACCACGTGCGCCTTCTCCTG+CGG 0.604226 5.2:-48464521 None:intergenic
TCTCCGACGACGGAAGCCGT+CGG 0.605600 5.2:+48464573 MS.gene65682:CDS
CGTCGGTCGTAGCCGAGTGG+GGG 0.611250 5.2:+48464590 MS.gene65682:CDS
GACGATCACATCTGGTGATG+CGG 0.617092 5.2:-48464497 None:intergenic
AGCCGTCGGTCGTAGCCGAG+TGG 0.624990 5.2:+48464587 MS.gene65682:CDS
TCCGCAGGAGAAGGCGCACG+TGG 0.637444 5.2:+48464520 MS.gene65682:CDS
TGACGCGGATAATACTTGGT+GGG 0.643095 5.2:+48464747 MS.gene65682:CDS
TTTGTTTATCATGTCATACG+TGG 0.648765 5.2:-48464449 None:intergenic
ACGCTGCGCGTGGAATCTGG+CGG 0.651618 5.2:+48464628 MS.gene65682:CDS
AAGTGGTTGTGATTTGTGAA+GGG 0.653510 5.2:-48463934 None:intergenic
GCGCAGCGTGTTCCCCACCG+TGG 0.659108 5.2:-48464614 None:intergenic
GATTCCGTTACGTTACCTAA+CGG 0.659410 5.2:-48464724 None:intergenic
TTTAGATTTCGATGATTACG+AGG 0.676604 5.2:+48464414 MS.gene65682:CDS
GTGTTCGAAACTCAAAACGG+TGG 0.697096 5.2:+48464289 MS.gene65682:CDS
TAACGTAACGGAATCTGACG+CGG 0.701362 5.2:+48464732 MS.gene65682:CDS
TGTTCCGTTAGGTAACGTAA+CGG 0.710318 5.2:+48464720 MS.gene65682:CDS
ACATGCGCCTTCAACTTCTG+CGG 0.735945 5.2:+48464474 MS.gene65682:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAGTTTATGTTAGAGATGA+CGG + chr5.2:48464658-48464677 MS.gene65682:CDS 25.0%
!!! AAAGATTTTTTTCGAGTTAC+AGG + chr5.2:48464238-48464257 MS.gene65682:CDS 25.0%
ATTGTTACGGATGAATCTTA+CGG + chr5.2:48464784-48464803 MS.gene65682:CDS 30.0%
CTTAACAACGTTATCACTAT+TGG - chr5.2:48464365-48464384 None:intergenic 30.0%
GAAATTGAAAGCAATGGAAA+CGG - chr5.2:48463978-48463997 None:intergenic 30.0%
TGCTTTCAATTTCTGCAAAT+GGG + chr5.2:48463985-48464004 MS.gene65682:CDS 30.0%
TTGCTTTCAATTTCTGCAAA+TGG + chr5.2:48463984-48464003 MS.gene65682:CDS 30.0%
TTTAGATTTCGATGATTACG+AGG + chr5.2:48464414-48464433 MS.gene65682:CDS 30.0%
TTTGCAGAAATTGAAAGCAA+TGG - chr5.2:48463984-48464003 None:intergenic 30.0%
TTTGTTTATCATGTCATACG+TGG - chr5.2:48464452-48464471 None:intergenic 30.0%
! TGATAACGTTGTTAAGCTTT+GGG + chr5.2:48464369-48464388 MS.gene65682:CDS 30.0%
AACGTGTTCGAAACTCAAAA+CGG + chr5.2:48464286-48464305 MS.gene65682:CDS 35.0%
CTTGATGAAGATGACATAGA+TGG - chr5.2:48464214-48464233 None:intergenic 35.0%
GCTTTCAATTTCTGCAAATG+GGG + chr5.2:48463986-48464005 MS.gene65682:CDS 35.0%
TGCTGATGCTGTTATTGTTA+CGG + chr5.2:48464771-48464790 MS.gene65682:CDS 35.0%
TTGTTACGGATGAATCTTAC+GGG + chr5.2:48464785-48464804 MS.gene65682:CDS 35.0%
! AAGTGGTTGTGATTTGTGAA+GGG - chr5.2:48463937-48463956 None:intergenic 35.0%
! CGTTAGTTTATCTTCGTCAT+CGG + chr5.2:48464171-48464190 MS.gene65682:CDS 35.0%
! GACAGTATAGGATTTGGTTT+AGG + chr5.2:48464391-48464410 MS.gene65682:CDS 35.0%
! GTGATAACGTTGTTAAGCTT+TGG + chr5.2:48464368-48464387 MS.gene65682:CDS 35.0%
! GTTTTCGTTATCTTCGTCAT+CGG - chr5.2:48464143-48464162 None:intergenic 35.0%
! TCGTTGAGTCTATCGATTAA+TGG - chr5.2:48463872-48463891 None:intergenic 35.0%
! TGATTTGTGAAGGGAAAGAT+GGG - chr5.2:48463928-48463947 None:intergenic 35.0%
!!! AAGAAGCTTTTTTACGAAGG+TGG - chr5.2:48464078-48464097 None:intergenic 35.0%
AATCTGACGCGGATAATACT+TGG + chr5.2:48464743-48464762 MS.gene65682:CDS 40.0%
AGAAAAGTCAGTGTTCCGTT+AGG + chr5.2:48464709-48464728 MS.gene65682:CDS 40.0%
ATCACAACCACTTCCTTCAA+TGG + chr5.2:48463944-48463963 MS.gene65682:CDS 40.0%
CATAGACGACAACGTAGTAT+TGG + chr5.2:48464313-48464332 MS.gene65682:CDS 40.0%
CGAGTTACAGGAAACAGTTA+TGG + chr5.2:48464250-48464269 MS.gene65682:CDS 40.0%
GATTCCGTTACGTTACCTAA+CGG - chr5.2:48464727-48464746 None:intergenic 40.0%
GATTTCGATGATTACGAGGA+TGG + chr5.2:48464418-48464437 MS.gene65682:CDS 40.0%
GCAATGAATTGAAACCTGCT+TGG - chr5.2:48463898-48463917 None:intergenic 40.0%
TAATCTCGCAGTCGAGATAT+GGG + chr5.2:48464543-48464562 MS.gene65682:CDS 40.0%
TGAAAGCAATGGAAACGGAT+TGG - chr5.2:48463973-48463992 None:intergenic 40.0%
TGTTCCGTTAGGTAACGTAA+CGG + chr5.2:48464720-48464739 MS.gene65682:CDS 40.0%
TTGGATTCCATTGAAGGAAG+TGG - chr5.2:48463954-48463973 None:intergenic 40.0%
! GAAGTGGTTGTGATTTGTGA+AGG - chr5.2:48463938-48463957 None:intergenic 40.0%
! GTGATTTGTGAAGGGAAAGA+TGG - chr5.2:48463929-48463948 None:intergenic 40.0%
!! AACGGATTGGATTCCATTGA+AGG - chr5.2:48463960-48463979 None:intergenic 40.0%
!! CTTTGGGACAGTATAGGATT+TGG + chr5.2:48464385-48464404 MS.gene65682:CDS 40.0%
!! GTTAAGCTTTGGGACAGTAT+AGG + chr5.2:48464379-48464398 MS.gene65682:CDS 40.0%
!!! GGGAAGAAGCTTTTTTACGA+AGG - chr5.2:48464081-48464100 None:intergenic 40.0%
ACGAAGGTGGACGATGAAAA+TGG - chr5.2:48464065-48464084 None:intergenic 45.0%
AGACTCAACGATTTCCAAGC+AGG + chr5.2:48463881-48463900 MS.gene65682:CDS 45.0%
GAAATCATCGTCGTTGTCGA+AGG - chr5.2:48464116-48464135 None:intergenic 45.0%
GTAATCTCGCAGTCGAGATA+TGG + chr5.2:48464542-48464561 MS.gene65682:CDS 45.0%
GTCGAAGGTGATTGATGGAA+GGG - chr5.2:48464101-48464120 None:intergenic 45.0%
GTGTTCGAAACTCAAAACGG+TGG + chr5.2:48464289-48464308 MS.gene65682:CDS 45.0%
TCGTTGTCGAAGGTGATTGA+TGG - chr5.2:48464106-48464125 None:intergenic 45.0%
TGACGCGGATAATACTTGGT+GGG + chr5.2:48464747-48464766 MS.gene65682:CDS 45.0%
TGTCGAAGGTGATTGATGGA+AGG - chr5.2:48464102-48464121 None:intergenic 45.0%
TTCGTTATCTTCGTCATCGG+AGG - chr5.2:48464140-48464159 None:intergenic 45.0%
! TAACGTAACGGAATCTGACG+CGG + chr5.2:48464732-48464751 MS.gene65682:CDS 45.0%
!! GGTGACTTTGTTGATGATGC+TGG - chr5.2:48464044-48464063 None:intergenic 45.0%
ACATGCGCCTTCAACTTCTG+CGG + chr5.2:48464474-48464493 MS.gene65682:CDS 50.0%
CTGACGCGGATAATACTTGG+TGG + chr5.2:48464746-48464765 MS.gene65682:CDS 50.0%
GGACGTCAACGTTTGACAGT+TGG + chr5.2:48464679-48464698 MS.gene65682:CDS 50.0%
! GACGATCACATCTGGTGATG+CGG - chr5.2:48464500-48464519 None:intergenic 50.0%
! GCAGAAGTTGAAGGCGCATG+TGG - chr5.2:48464475-48464494 None:intergenic 55.0%
AACACGCTGCGCGTGGAATC+TGG + chr5.2:48464625-48464644 MS.gene65682:CDS 60.0%
CCAGATGTGATCGTCTCCGC+AGG + chr5.2:48464505-48464524 MS.gene65682:CDS 60.0%
CCTGCGGAGACGATCACATC+TGG - chr5.2:48464508-48464527 None:intergenic 60.0%
GATGCGGCCGCAGAAGTTGA+AGG - chr5.2:48464484-48464503 None:intergenic 60.0%
GTGATCGTCTCCGCAGGAGA+AGG + chr5.2:48464511-48464530 MS.gene65682:CDS 60.0%
ACCACGTGCGCCTTCTCCTG+CGG - chr5.2:48464524-48464543 None:intergenic 65.0%
ACGCTGCGCGTGGAATCTGG+CGG + chr5.2:48464628-48464647 MS.gene65682:CDS 65.0%
GGGACACTCGTCTCCGACGA+CGG + chr5.2:48464563-48464582 MS.gene65682:CDS 65.0%
TCTCCGACGACGGAAGCCGT+CGG + chr5.2:48464573-48464592 MS.gene65682:CDS 65.0%
AGCCGTCGGTCGTAGCCGAG+TGG + chr5.2:48464587-48464606 MS.gene65682:CDS 70.0%
CCCCACTCGGCTACGACCGA+CGG - chr5.2:48464592-48464611 None:intergenic 70.0%
CCGTCGGTCGTAGCCGAGTG+GGG + chr5.2:48464589-48464608 MS.gene65682:CDS 70.0%
CGACCGACGGCTTCCGTCGT+CGG - chr5.2:48464579-48464598 None:intergenic 70.0%
CGCAGCGTGTTCCCCACCGT+GGG - chr5.2:48464616-48464635 None:intergenic 70.0%
CGTCGGTCGTAGCCGAGTGG+GGG + chr5.2:48464590-48464609 MS.gene65682:CDS 70.0%
GCCGTCGGTCGTAGCCGAGT+GGG + chr5.2:48464588-48464607 MS.gene65682:CDS 70.0%
! TCCGCAGGAGAAGGCGCACG+TGG + chr5.2:48464520-48464539 MS.gene65682:CDS 70.0%
CGTAGCCGAGTGGGGGCCCA+CGG + chr5.2:48464597-48464616 MS.gene65682:CDS 75.0%
GCGCAGCGTGTTCCCCACCG+TGG - chr5.2:48464617-48464636 None:intergenic 75.0%
GGTGGGGAACACGCTGCGCG+TGG + chr5.2:48464618-48464637 MS.gene65682:CDS 75.0%
! AGCCGAGTGGGGGCCCACGG+TGG + chr5.2:48464600-48464619 MS.gene65682:CDS 80.0%
! CCCCACCGTGGGCCCCCACT+CGG - chr5.2:48464605-48464624 None:intergenic 80.0%
! CCGAGTGGGGGCCCACGGTG+GGG + chr5.2:48464602-48464621 MS.gene65682:CDS 80.0%
! GCCGAGTGGGGGCCCACGGT+GGG + chr5.2:48464601-48464620 MS.gene65682:CDS 80.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 48463860 48464816 48463860 ID=MS.gene65682
chr5.2 mRNA 48463860 48464816 48463860 ID=MS.gene65682.t1;Parent=MS.gene65682
chr5.2 exon 48463860 48464816 48463860 ID=MS.gene65682.t1.exon1;Parent=MS.gene65682.t1
chr5.2 CDS 48463860 48464816 48463860 ID=cds.MS.gene65682.t1;Parent=MS.gene65682.t1
Gene Sequence

>MS.gene65682

ATGCAAGTTCCATTAATCGATAGACTCAACGATTTCCAAGCAGGTTTCAATTCATTGCAACAAAACCCATCTTTCCCTTCACAAATCACAACCACTTCCTTCAATGGAATCCAATCCGTTTCCATTGCTTTCAATTTCTGCAAATGGGGTGCTGTCATTCTTGCTTTAGTCGCAACTTTCACCAGCATCATCAACAAAGTCACCATTTTCATCGTCCACCTTCGTAAAAAAGCTTCTTCCCTTCCATCAATCACCTTCGACAACGACGATGATTTCACCTCCGATGACGAAGATAACGAAAACGATGACATCGTTAGTTTATCTTCGTCATCGGAATTCGAAGACGATGAACCATCTATGTCATCTTCATCAAGCTTCAAAGATTTTTTTCGAGTTACAGGAAACAGTTATGGTTACGATGTAAACAACGTGTTCGAAACTCAAAACGGTGGACATAGACGACAACGTAGTATTGGTGATTTTTTTTCTCTTTTAGAATTAGCCAATAGTGATAACGTTGTTAAGCTTTGGGACAGTATAGGATTTGGTTTAGGTTTAGATTTCGATGATTACGAGGATGGAGTTGTATCCACGTATGACATGATAAACAAACCACATGCGCCTTCAACTTCTGCGGCCGCATCACCAGATGTGATCGTCTCCGCAGGAGAAGGCGCACGTGGTAATCTCGCAGTCGAGATATGGGACACTCGTCTCCGACGACGGAAGCCGTCGGTCGTAGCCGAGTGGGGGCCCACGGTGGGGAACACGCTGCGCGTGGAATCTGGCGGCGTTCAGAAAGTTTATGTTAGAGATGACGGACGTCAACGTTTGACAGTTGGTGATATGAGAAAAGTCAGTGTTCCGTTAGGTAACGTAACGGAATCTGACGCGGATAATACTTGGTGGGATGCTGATGCTGTTATTGTTACGGATGAATCTTACGGGAAACATTAA

Protein sequence

>MS.gene65682.t1

MQVPLIDRLNDFQAGFNSLQQNPSFPSQITTTSFNGIQSVSIAFNFCKWGAVILALVATFTSIINKVTIFIVHLRKKASSLPSITFDNDDDFTSDDEDNENDDIVSLSSSSEFEDDEPSMSSSSSFKDFFRVTGNSYGYDVNNVFETQNGGHRRQRSIGDFFSLLELANSDNVVKLWDSIGFGLGLDFDDYEDGVVSTYDMINKPHAPSTSAAASPDVIVSAGEGARGNLAVEIWDTRLRRRKPSVVAEWGPTVGNTLRVESGGVQKVYVRDDGRQRLTVGDMRKVSVPLGNVTESDADNTWWDADAVIVTDESYGKH