AlfalfaGEDB | MS.gene66452

M. sativa cultivar XinJiangDaYe / MS.gene66452

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF00514.26 PF14664.9

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66452.t1	XP_003589079.2	98.8	344	4	0	1	344	1	344	1.20E-62	250.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66452.t1	A2ZLU6	53.2	77	35	1	51	127	360	435	6.1e-10	66.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66452.t1	G7ICC9	98.8	344	4	0	1	344	1	344	8.9e-63	250.4

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049283	MS.gene66452	0.80159	8.38E-49	-1.69E-46
MS.gene050271	MS.gene66452	0.816075	6.67E-52	-1.69E-46
MS.gene051699	MS.gene66452	0.800903	1.16E-48	-1.69E-46
MS.gene051832	MS.gene66452	0.816775	4.65E-52	-1.69E-46
MS.gene052239	MS.gene66452	-0.801704	7.94E-49	-1.69E-46
MS.gene053188	MS.gene66452	0.804357	2.25E-49	-1.69E-46
MS.gene053189	MS.gene66452	0.834419	3.03E-56	-1.69E-46
MS.gene053298	MS.gene66452	0.805084	1.58E-49	-1.69E-46
MS.gene053575	MS.gene66452	0.804146	2.49E-49	-1.69E-46
MS.gene053963	MS.gene66452	0.839909	1.19E-57	-1.69E-46

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66452.t1	MTR_1g018180	98.837	344	4	0	1	344	1	344	0.0	683
MS.gene66452.t1	MTR_3g103050	75.831	331	73	4	16	340	5	334	6.14e-177	494
MS.gene66452.t1	MTR_1g069845	51.408	284	137	1	61	344	323	605	1.39e-85	270
MS.gene66452.t1	MTR_2g026130	52.448	286	133	1	61	343	175	460	7.81e-85	264
MS.gene66452.t1	MTR_1g069845	51.408	284	137	1	61	344	440	722	3.23e-84	270
MS.gene66452.t1	MTR_1g069845	51.408	284	137	1	61	344	444	726	4.50e-84	269
MS.gene66452.t1	MTR_3g466220	53.957	278	127	1	61	338	532	808	2.52e-81	264
MS.gene66452.t1	MTR_3g466220	53.957	278	127	1	61	338	553	829	2.88e-81	264
MS.gene66452.t1	MTR_7g097020	50.360	278	137	1	61	338	482	758	1.49e-77	253
MS.gene66452.t1	MTR_4g485520	48.188	276	143	0	61	336	205	480	8.94e-74	236

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene66452.t1	AT3G01400	81.250	336	60	2	5	339	10	343	0.0	548
MS.gene66452.t1	AT5G58680	73.669	338	83	4	5	338	6	341	4.17e-177	495
MS.gene66452.t1	AT2G23140	49.329	298	144	2	47	338	334	630	3.50e-85	270
MS.gene66452.t1	AT2G23140	49.329	298	144	2	47	338	334	630	3.50e-85	270
MS.gene66452.t1	AT2G23140	47.840	324	158	4	25	338	500	822	2.95e-83	269
MS.gene66452.t1	AT2G23140	47.840	324	158	4	25	338	497	819	3.02e-83	269
MS.gene66452.t1	AT2G23140	47.840	324	158	4	25	338	497	819	3.02e-83	269
MS.gene66452.t1	AT4G16490	51.579	285	136	1	61	343	185	469	1.01e-82	259
MS.gene66452.t1	AT3G54790	50.714	280	136	2	63	341	440	718	1.41e-78	254
MS.gene66452.t1	AT5G67340	49.822	281	139	2	61	340	423	702	2.12e-78	254

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Find 87 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAACTAATAGCTTCTTC+CGG	0.451795	1.4:+4871503	MS.gene66452:CDS
AACAGAGAGTTACACATACA+TGG	0.531557	1.4:+4871106	MS.gene66452:CDS
AACTAACGGTTTAATAGCTC+CGG	0.461417	1.4:-4871522	None:intergenic
AAGATGAGAGCGGTGAAAGC+GGG	0.475585	1.4:+4871834	MS.gene66452:CDS
AAGGCGGCGATTGGAAGATC+AGG	0.482551	1.4:+4871711	MS.gene66452:CDS
AAGGTTGCGTTGGTGGAAGA+AGG	0.538669	1.4:+4871957	MS.gene66452:CDS
AAGTTGACGGATGAGGTCAT+CGG	0.659006	1.4:-4871270	None:intergenic
AATTTCGTTAGTTACTTCAC+AGG	0.505937	1.4:+4871418	MS.gene66452:CDS
ACAGAGAGTTACACATACAT+GGG	0.672322	1.4:+4871107	MS.gene66452:CDS
ACAGATTTGTGAGGATAGCG+CGG	0.684005	1.4:+4872055	MS.gene66452:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAACTAATAGCTTCTTC+CGG	+	chr1.4:4871503-4871522	MS.gene66452:CDS	30.0%
AATTTCGTTAGTTACTTCAC+AGG	+	chr1.4:4871418-4871437	MS.gene66452:CDS	30.0%
GAAAAGAAGAGTTTCGATTT+AGG	-	chr1.4:4872422-4872441	None:intergenic	30.0%
GAATTTAACGCTCTAACTAA+CGG	-	chr1.4:4871539-4871558	None:intergenic	30.0%
GCCGAGTAAAAACAAAATTT+AGG	-	chr1.4:4872308-4872327	None:intergenic	30.0%
TATTCTGTTACAGATTTGTG+AGG	+	chr1.4:4872046-4872065	MS.gene66452:CDS	30.0%
TTCATTATTGTCCAAACATG+TGG	-	chr1.4:4873009-4873028	None:intergenic	30.0%
TTTCTGTTCTTCGATTGAAT+CGG	-	chr1.4:4871312-4871331	None:intergenic	30.0%
AACAGAGAGTTACACATACA+TGG	+	chr1.4:4871106-4871125	MS.gene66452:CDS	35.0%
AACTAACGGTTTAATAGCTC+CGG	-	chr1.4:4871525-4871544	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	4871101	4873161	4871101	ID=MS.gene66452
chr1.4	mRNA	4871101	4873161	4871101	ID=MS.gene66452.t1;Parent=MS.gene66452
chr1.4	exon	4871101	4871613	4871101	ID=MS.gene66452.t1.exon1;Parent=MS.gene66452.t1
chr1.4	CDS	4871101	4871613	4871101	ID=cds.MS.gene66452.t1;Parent=MS.gene66452.t1
chr1.4	exon	4871699	4872163	4871699	ID=MS.gene66452.t1.exon2;Parent=MS.gene66452.t1
chr1.4	CDS	4871699	4872163	4871699	ID=cds.MS.gene66452.t1;Parent=MS.gene66452.t1
chr1.4	exon	4873105	4873161	4873105	ID=MS.gene66452.t1.exon3;Parent=MS.gene66452.t1
chr1.4	CDS	4873105	4873161	4873105	ID=cds.MS.gene66452.t1;Parent=MS.gene66452.t1

Gene Sequence

>MS.gene66452

ATGGGAACAGAGAGTTACACATACATGGGACGAAGTTTCAAAGAATTCACCATCAACGACGATTCAACTTCCTCCGCTTTCAGTGACTGCAACAGTGACAGATCCGGCGAATTCGCCACCACTTCTTCTACCTCCCGACGACTCTTCCTCGCCTGCGCTTCCGAAAATTCCGATGACCTCATCCGTCAACTTGTCTCCGATCTTCACTCCGATTCAATCGAAGAACAGAAACAAGCTGCCATGGAAATTCGATTGCTCGCAAAGAATAAACCGGAAAATCGAATCAAAATTGCTAAAGCTGGCGCGATTAAACCATTAATTTCGTTAGTTACTTCACAGGACCTGCAACTTCAAGAATACGGTGTTACCGCGATTTTGAATCTCTCTTTGTGTGACGAGAATAAAGAACTAATAGCTTCTTCCGGAGCTATTAAACCGTTAGTTAGAGCGTTAAATTCAGGAACATCAACAGCTAAAGAGAACGCTGCTTGTGCTCTCCTTCGTCTATCTCAGGTTATTAGATGTAATAGTTAGTTATACCGGTTAATTATTAGTTGTTGTCATTGATTATTGAAAGTTCTAACGGTAATGATTACAGGTGGAAGAGAACAAGGCGGCGATTGGAAGATCAGGTGCAATTCCGTTATTGGTGAATCTTCTAGGAAGTGGAGGAATACGCGGGAAGAAGGACGCGTCTACGGCGCTGTATACTCTGTGTTCTGTGAAGGAGAATAAGATGAGAGCGGTGAAAGCGGGGATAATGAAAGTGTTGGTTGAGTTGATGGCGGATTTTGAATCGAATATGGTGGATAAATCGGCTTATGTTTTGAGTGTGTTGGTATCGGTGCCGGAGGCGAAGGTTGCGTTGGTGGAAGAAGGTGGTGTGCCGGTGTTGGTTGAGATTGTGGAGGTTGGATCGCAGAGACAGAAGGAAATTGCGGCGGTTATTCTGTTACAGATTTGTGAGGATAGCGCGGCGGTTCGATCCATGGTGGCTCGTGAAGGTGCCATTCCTCCTTTGGTTGCTTTGACGCAGTCCGGCACAAATCGCGCCAAACAAAAGGTAGAATATTATTATCTTTACTTTTTTTTTATTAATTATATATTGATAATTTGATATAGATGTTAATTATTCAATAGCGTAATTTTTCAAAAAAAAATTATTCAATAATCTAATTTTCGAATTAGATGACTCGAGTCTTTTCCTAAATTTTGTTTTTACTCGGCTAGTTCGATTTAAATTATTGGCTCAAATTTAGTCTTGACTTATCTCTTTCAGCGTAACTAAATGTTCAAATGTCGTTAGTTTGAACTCAGCCCTAAATCGAAACTCTTCTTTTCTATTTGTTTTCAATTTAATTAGAGGTCATCAATTCAAATTTAGTACAATTAATTAACTATATTTCAATGGTTCGAATTCTTCATTGATTGGTTCGAATGTAATTTTGATCTTTTTTTTTAACAAAAATGTAATTTTGATCTTGAAAATGCAGCAGCATAAAAAAAATTACACACATTGCCATTTTAGTTTAAATATTAAATGAATTATTCAGCTTGAATTCAGCATTGCTCCCTCAACTTGCGGTAGCTGACGTTGCATGGTGCCGTTTATTGCTTTGGTGGAAGAACATGTAATGCCACGTCGACTCATTGTTCTTGCAGAATCGACCTCCACCCACCGCAATTTTTGCCCTATTGTCTAATTGTGTCTAATTAAAATGCTCCTCATTGCGTTCATACGACAAACCTGCATTATTATTAATTTATTATTATTATCATGTAGTAATATATTTCGTAAAATCTTGAATATTCTTATTGAATGGGTATAGCATTATTTTAAGAACTATTTATGTCGTGGAAGCTTATCATGCGGCTTGTGTTGTGCTTTTTTTTTCTATAGTAGTGCCACATGTTTGGACAATAATGAAATTTAGAAATCACGTTAATATCTCAATCATTTCATTTTTTTTAATAATAATGTTTTTCTGTTTTTGGTGGGTTCAGGCTGAGAAACTAATTGAGCTTCTGAGGCAACCAAGATCTACACGTACATCAGAATGA

Protein sequence

>MS.gene66452.t1

MGTESYTYMGRSFKEFTINDDSTSSAFSDCNSDRSGEFATTSSTSRRLFLACASENSDDLIRQLVSDLHSDSIEEQKQAAMEIRLLAKNKPENRIKIAKAGAIKPLISLVTSQDLQLQEYGVTAILNLSLCDENKELIASSGAIKPLVRALNSGTSTAKENAACALLRLSQVEENKAAIGRSGAIPLLVNLLGSGGIRGKKDASTALYTLCSVKENKMRAVKAGIMKVLVELMADFESNMVDKSAYVLSVLVSVPEAKVALVEEGGVPVLVEIVEVGSQRQKEIAAVILLQICEDSAAVRSMVAREGAIPPLVALTQSGTNRAKQKAEKLIELLRQPRSTRTSE