AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66452

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66452.t1 MTR_1g018180 98.837 344 4 0 1 344 1 344 0.0 683
MS.gene66452.t1 MTR_3g103050 75.831 331 73 4 16 340 5 334 6.14e-177 494
MS.gene66452.t1 MTR_1g069845 51.408 284 137 1 61 344 323 605 1.39e-85 270
MS.gene66452.t1 MTR_2g026130 52.448 286 133 1 61 343 175 460 7.81e-85 264
MS.gene66452.t1 MTR_1g069845 51.408 284 137 1 61 344 440 722 3.23e-84 270
MS.gene66452.t1 MTR_1g069845 51.408 284 137 1 61 344 444 726 4.50e-84 269
MS.gene66452.t1 MTR_3g466220 53.957 278 127 1 61 338 532 808 2.52e-81 264
MS.gene66452.t1 MTR_3g466220 53.957 278 127 1 61 338 553 829 2.88e-81 264
MS.gene66452.t1 MTR_7g097020 50.360 278 137 1 61 338 482 758 1.49e-77 253
MS.gene66452.t1 MTR_4g485520 48.188 276 143 0 61 336 205 480 8.94e-74 236
MS.gene66452.t1 MTR_4g485520 48.188 276 143 0 61 336 373 648 1.01e-72 238
MS.gene66452.t1 MTR_4g063800 44.366 284 158 0 61 344 353 636 2.04e-67 224
MS.gene66452.t1 MTR_1g094215 44.203 276 154 0 61 336 162 437 7.32e-63 207
MS.gene66452.t1 MTR_7g059405 42.652 279 154 2 56 334 360 632 9.23e-62 208
MS.gene66452.t1 MTR_1g094215 44.203 276 154 0 61 336 345 620 4.03e-61 206
MS.gene66452.t1 MTR_7g078330 40.283 283 168 1 54 336 361 642 6.79e-61 206
MS.gene66452.t1 MTR_5g083030 41.034 290 154 6 64 340 408 693 6.17e-51 180
MS.gene66452.t1 MTR_7g116600 38.078 281 163 6 72 343 182 460 1.90e-39 145
MS.gene66452.t1 MTR_5g069340 35.540 287 179 5 61 341 34 320 1.31e-37 138
MS.gene66452.t1 MTR_7g053260 34.909 275 168 7 70 336 180 451 8.15e-36 135
MS.gene66452.t1 MTR_2g018010 36.918 279 168 6 64 336 399 675 3.32e-34 133
MS.gene66452.t1 MTR_5g079550 33.916 286 182 5 55 335 14 297 1.05e-29 117
MS.gene66452.t1 MTR_3g078340 28.727 275 191 2 64 335 214 486 1.98e-28 115
MS.gene66452.t1 MTR_3g078160 33.692 279 171 9 74 342 390 664 8.51e-28 114
MS.gene66452.t1 MTR_3g078160 33.692 279 171 9 74 342 390 664 1.21e-27 114
MS.gene66452.t1 MTR_8g080280 30.938 320 199 9 30 338 404 712 1.23e-27 114
MS.gene66452.t1 MTR_4g107010 29.480 346 225 10 7 343 337 672 4.61e-25 106
MS.gene66452.t1 MTR_3g106140 29.492 295 194 6 61 343 110 402 1.05e-24 104
MS.gene66452.t1 MTR_1g079450 33.564 289 181 6 63 341 156 443 1.97e-23 100
MS.gene66452.t1 MTR_3g085610 33.083 266 166 8 82 338 448 710 4.13e-23 100
MS.gene66452.t1 MTR_3g067685 34.146 287 179 6 61 339 41 325 5.57e-23 99.0
MS.gene66452.t1 MTR_1g014420 29.617 287 185 9 63 335 130 413 4.68e-22 97.4
MS.gene66452.t1 MTR_4g091880 33.171 205 130 5 142 343 147 347 3.18e-19 87.8
MS.gene66452.t1 MTR_4g091880 32.536 209 134 5 138 343 171 375 3.96e-19 87.8
MS.gene66452.t1 MTR_6g004790 30.035 283 150 6 64 339 44 285 7.03e-19 86.7
MS.gene66452.t1 MTR_8g032570 29.237 236 155 6 101 331 261 489 7.17e-19 88.2
MS.gene66452.t1 MTR_6g008170 24.723 271 199 2 64 331 241 509 8.88e-19 87.8
MS.gene66452.t1 MTR_8g032570 29.237 236 155 6 101 331 238 466 9.41e-19 87.8
MS.gene66452.t1 MTR_4g028960 32.143 280 165 8 72 336 396 665 7.02e-18 85.1
MS.gene66452.t1 MTR_2g011140 29.787 282 186 6 60 335 104 379 2.94e-17 82.4
MS.gene66452.t1 MTR_3g078160 38.750 160 92 5 74 230 390 546 2.18e-16 80.5
MS.gene66452.t1 MTR_7g094910 28.079 203 138 2 61 263 269 463 2.12e-14 74.7
MS.gene66452.t1 MTR_7g094910 28.079 203 138 2 61 263 269 463 2.23e-14 74.7
MS.gene66452.t1 MTR_7g094900 28.079 203 138 2 61 263 288 482 2.31e-14 74.7
MS.gene66452.t1 MTR_1g104870 26.400 250 176 3 74 323 295 536 4.62e-14 73.6
MS.gene66452.t1 MTR_5g034440 26.042 288 205 4 64 343 386 673 1.22e-13 72.4
MS.gene66452.t1 MTR_7g076220 23.620 326 205 7 61 344 156 479 2.07e-13 71.6
MS.gene66452.t1 MTR_5g005940 26.400 250 176 3 74 323 292 533 9.44e-13 69.7
MS.gene66452.t1 MTR_5g005940 26.400 250 176 3 74 323 292 533 1.41e-12 68.9
MS.gene66452.t1 MTR_7g007780 33.793 145 93 1 99 240 597 741 1.02e-11 66.6
MS.gene66452.t1 MTR_4g085720 35.000 140 89 2 198 335 251 390 1.15e-11 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66452.t1 AT3G01400 81.250 336 60 2 5 339 10 343 0.0 548
MS.gene66452.t1 AT5G58680 73.669 338 83 4 5 338 6 341 4.17e-177 495
MS.gene66452.t1 AT2G23140 49.329 298 144 2 47 338 334 630 3.50e-85 270
MS.gene66452.t1 AT2G23140 49.329 298 144 2 47 338 334 630 3.50e-85 270
MS.gene66452.t1 AT2G23140 47.840 324 158 4 25 338 500 822 2.95e-83 269
MS.gene66452.t1 AT2G23140 47.840 324 158 4 25 338 497 819 3.02e-83 269
MS.gene66452.t1 AT2G23140 47.840 324 158 4 25 338 497 819 3.02e-83 269
MS.gene66452.t1 AT4G16490 51.579 285 136 1 61 343 185 469 1.01e-82 259
MS.gene66452.t1 AT3G54790 50.714 280 136 2 63 341 440 718 1.41e-78 254
MS.gene66452.t1 AT5G67340 49.822 281 139 2 61 340 423 702 2.12e-78 254
MS.gene66452.t1 AT5G67340 33.125 160 104 3 61 219 548 705 9.11e-11 63.5
MS.gene66452.t1 AT5G67340 49.822 281 139 2 61 340 423 702 2.12e-78 254
MS.gene66452.t1 AT5G67340 33.125 160 104 3 61 219 548 705 9.11e-11 63.5
MS.gene66452.t1 AT3G54790 50.714 280 136 2 63 341 476 754 5.25e-78 254
MS.gene66452.t1 AT3G54790 50.714 280 136 2 63 341 476 754 5.25e-78 254
MS.gene66452.t1 AT1G23030 45.704 291 155 2 49 336 319 609 1.52e-71 234
MS.gene66452.t1 AT1G71020 47.482 278 144 2 61 336 195 472 5.28e-69 224
MS.gene66452.t1 AT1G71020 47.482 278 144 2 61 336 343 620 7.81e-68 224
MS.gene66452.t1 AT3G46510 44.118 272 152 0 61 332 354 625 7.79e-66 219
MS.gene66452.t1 AT2G28830 44.322 273 150 2 61 331 285 557 1.27e-64 214
MS.gene66452.t1 AT3G54850 46.403 278 147 2 60 336 346 622 9.22e-61 206
MS.gene66452.t1 AT5G42340 41.667 276 161 0 59 334 379 654 1.37e-53 187
MS.gene66452.t1 AT3G07360 34.146 287 177 7 59 336 34 317 3.01e-36 134
MS.gene66452.t1 AT3G07360 34.146 287 177 7 59 336 34 317 3.01e-36 134
MS.gene66452.t1 AT3G07360 34.146 287 177 7 59 336 34 317 3.01e-36 134
MS.gene66452.t1 AT3G07360 34.146 287 177 7 59 336 34 317 3.01e-36 134
MS.gene66452.t1 AT3G07360 34.146 287 177 7 59 336 34 317 3.01e-36 134
MS.gene66452.t1 AT3G07360 34.146 287 177 7 59 336 34 317 3.01e-36 134
MS.gene66452.t1 AT3G07360 34.146 287 177 7 59 336 34 317 3.01e-36 134
MS.gene66452.t1 AT3G07360 34.146 287 177 7 59 336 34 317 3.01e-36 134
MS.gene66452.t1 AT3G07360 34.146 287 177 7 59 336 34 317 3.01e-36 134
MS.gene66452.t1 AT3G07360 34.146 287 177 7 59 336 169 452 3.14e-35 134
MS.gene66452.t1 AT4G12710 35.587 281 170 8 68 338 55 334 4.62e-32 124
MS.gene66452.t1 AT3G03440 32.872 289 185 6 61 342 66 352 6.00e-30 118
MS.gene66452.t1 AT1G29340 38.489 278 165 6 64 336 411 687 2.13e-29 119
MS.gene66452.t1 AT1G67530 32.975 279 177 6 71 341 436 712 6.40e-28 115
MS.gene66452.t1 AT1G67530 32.975 279 177 6 71 341 436 712 6.40e-28 115
MS.gene66452.t1 AT1G67530 32.975 279 177 6 71 341 436 712 6.40e-28 115
MS.gene66452.t1 AT5G40140 31.047 277 183 4 64 335 233 506 2.71e-27 112
MS.gene66452.t1 AT5G62560 30.216 278 187 4 63 335 238 513 2.88e-27 112
MS.gene66452.t1 AT5G14510 34.050 279 173 7 64 335 5 279 2.79e-25 104
MS.gene66452.t1 AT1G27910 31.126 302 192 7 51 338 407 706 1.01e-24 105
MS.gene66452.t1 AT1G24330 31.939 263 170 6 83 338 448 708 3.13e-23 101
MS.gene66452.t1 AT1G24330 31.939 263 170 6 83 338 448 708 3.13e-23 101
MS.gene66452.t1 AT4G31890 31.970 269 168 8 79 335 163 428 1.54e-22 99.0
MS.gene66452.t1 AT4G31890 31.970 269 168 8 79 335 163 428 1.54e-22 99.0
MS.gene66452.t1 AT4G31890 31.970 269 168 8 79 335 163 428 1.54e-22 99.0
MS.gene66452.t1 AT5G18320 28.713 303 190 9 57 336 149 448 9.76e-21 93.2
MS.gene66452.t1 AT5G65200 26.573 286 193 4 64 336 229 510 2.38e-20 92.4
MS.gene66452.t1 AT4G21350 32.042 284 185 5 55 334 88 367 3.93e-20 90.5
MS.gene66452.t1 AT3G47820 30.545 275 176 7 71 335 218 487 7.81e-20 90.9
MS.gene66452.t1 AT1G10560 28.621 290 195 7 64 342 393 681 1.21e-19 90.5
MS.gene66452.t1 AT2G25130 29.699 266 173 7 82 335 132 395 2.85e-19 89.0
MS.gene66452.t1 AT5G01830 30.657 274 173 8 72 336 380 645 9.23e-19 87.8
MS.gene66452.t1 AT1G60190 27.208 283 191 9 74 342 389 670 3.91e-17 83.2
MS.gene66452.t1 AT5G13060 25.941 239 155 3 61 292 311 534 5.31e-14 73.6
MS.gene66452.t1 AT5G50900 28.679 265 180 5 62 320 147 408 1.75e-13 71.6
MS.gene66452.t1 AT2G27430 26.689 296 202 6 55 341 73 362 2.84e-13 70.9
MS.gene66452.t1 AT3G19380 27.863 262 171 7 72 318 121 379 2.23e-12 68.2
MS.gene66452.t1 AT1G77460 29.317 249 156 7 97 335 467 705 2.62e-12 68.6
MS.gene66452.t1 AT1G77460 29.317 249 156 7 97 335 467 705 2.62e-12 68.6
MS.gene66452.t1 AT1G77460 29.317 249 156 7 97 335 467 705 2.62e-12 68.6
MS.gene66452.t1 AT1G77460 29.317 249 156 7 97 335 467 705 2.62e-12 68.6
MS.gene66452.t1 AT1G01830 27.755 245 171 5 48 287 155 398 2.96e-12 68.2
MS.gene66452.t1 AT1G01830 27.755 245 171 5 48 287 155 398 2.96e-12 68.2
MS.gene66452.t1 AT1G01830 27.755 245 171 5 48 287 155 398 2.96e-12 68.2
MS.gene66452.t1 AT2G44900 33.103 145 94 1 99 240 608 752 7.42e-12 67.0
MS.gene66452.t1 AT2G44900 33.103 145 94 1 99 240 608 752 7.42e-12 67.0
MS.gene66452.t1 AT3G60350 34.932 146 90 2 99 240 599 743 3.67e-11 65.1
MS.gene66452.t1 AT3G60350 34.932 146 90 2 99 240 599 743 3.67e-11 65.1
MS.gene66452.t1 AT1G61350 23.404 282 163 9 58 330 143 380 6.69e-11 63.9
MS.gene66452.t1 AT5G18330 27.778 270 169 8 57 306 142 405 8.26e-11 63.2

Find 87 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCGTTATTGGTGAATCTTCT+AGG 0.217498 1.4:+4871741 MS.gene66452:CDS
GAAGTAACTAACGAAATTAA+TGG 0.228085 1.4:-4871413 None:intergenic
ATCAGGTGCAATTCCGTTAT+TGG 0.234218 1.4:+4871728 MS.gene66452:CDS
TGAAGGTGCCATTCCTCCTT+TGG 0.322339 1.4:+4872100 MS.gene66452:CDS
TTAGTTAGAGCGTTAAATTC+AGG 0.337381 1.4:+4871539 MS.gene66452:CDS
TTGGTTGAGATTGTGGAGGT+TGG 0.376021 1.4:+4871993 MS.gene66452:CDS
CACCGTATTCTTGAAGTTGC+AGG 0.379035 1.4:-4871442 None:intergenic
GAAAGTGTTGGTTGAGTTGA+TGG 0.395341 1.4:+4871863 MS.gene66452:CDS
ATCGAATATGGTGGATAAAT+CGG 0.413047 1.4:+4871896 MS.gene66452:CDS
TTTCTGTTCTTCGATTGAAT+CGG 0.431111 1.4:-4871309 None:intergenic
GGAAGTTGAATCGTCGTTGA+TGG 0.431808 1.4:-4871150 None:intergenic
TGCTCTCCTTCGTCTATCTC+AGG 0.438091 1.4:+4871592 MS.gene66452:CDS
CGAATCAAAATTGCTAAAGC+TGG 0.447290 1.4:+4871380 MS.gene66452:CDS
TGGAGGAATACGCGGGAAGA+AGG 0.449541 1.4:+4871767 MS.gene66452:CDS
AAAGAACTAATAGCTTCTTC+CGG 0.451795 1.4:+4871503 MS.gene66452:CDS
CACAAATCGCGCCAAACAAA+AGG 0.454708 1.4:+4872142 MS.gene66452:CDS
AACTAACGGTTTAATAGCTC+CGG 0.461417 1.4:-4871522 None:intergenic
TTGGTTGCTTTGACGCAGTC+CGG 0.462970 1.4:+4872119 MS.gene66452:CDS
CGCAGGCGAGGAAGAGTCGT+CGG 0.463723 1.4:-4871235 None:intergenic
GGAGGCGAAGGTTGCGTTGG+TGG 0.465398 1.4:+4871950 MS.gene66452:CDS
TTGTTTGGCGCGATTTGTGC+CGG 0.465975 1.4:-4872138 None:intergenic
GAGTGTGTTGGTATCGGTGC+CGG 0.474240 1.4:+4871929 MS.gene66452:CDS
TTGGTGAATCTTCTAGGAAG+TGG 0.474246 1.4:+4871747 MS.gene66452:CDS
CCTAGAAGATTCACCAATAA+CGG 0.474649 1.4:-4871741 None:intergenic
AAGATGAGAGCGGTGAAAGC+GGG 0.475585 1.4:+4871834 MS.gene66452:CDS
GAAGAGAACAAGGCGGCGAT+TGG 0.476176 1.4:+4871702 MS.gene66452:CDS
AAGGCGGCGATTGGAAGATC+AGG 0.482551 1.4:+4871711 MS.gene66452:CDS
ACCAACGCAACCTTCGCCTC+CGG 0.483680 1.4:-4871948 None:intergenic
GGTATCGGTGCCGGAGGCGA+AGG 0.495124 1.4:+4871938 MS.gene66452:CDS
AATTTCGTTAGTTACTTCAC+AGG 0.505937 1.4:+4871418 MS.gene66452:CDS
GCAGGCGAGGAAGAGTCGTC+GGG 0.507232 1.4:-4871234 None:intergenic
GCCGGTGTTGGTTGAGATTG+TGG 0.507527 1.4:+4871986 MS.gene66452:CDS
TAGCGCGGCGGTTCGATCCA+TGG 0.519060 1.4:+4872070 MS.gene66452:CDS
TCTGATGTACGTGTAGATCT+TGG 0.520804 1.4:-4873135 None:intergenic
CGGAGACAAGTTGACGGATG+AGG 0.526478 1.4:-4871277 None:intergenic
CGGGAAGAAGGACGCGTCTA+CGG 0.528830 1.4:+4871779 MS.gene66452:CDS
GACTGCAACAGTGACAGATC+CGG 0.529495 1.4:+4871185 MS.gene66452:CDS
CAAAGCAACCAAAGGAGGAA+TGG 0.530629 1.4:-4872108 None:intergenic
AACAGAGAGTTACACATACA+TGG 0.531557 1.4:+4871106 MS.gene66452:CDS
AGAAGTGGTGGCGAATTCGC+CGG 0.533976 1.4:-4871204 None:intergenic
CGATCCATGGTGGCTCGTGA+AGG 0.535543 1.4:+4872083 MS.gene66452:CDS
ATTGCTCGCAAAGAATAAAC+CGG 0.537284 1.4:+4871352 MS.gene66452:CDS
GATTGAATCGGAGTGAAGAT+CGG 0.537748 1.4:-4871297 None:intergenic
AAGGTTGCGTTGGTGGAAGA+AGG 0.538669 1.4:+4871957 MS.gene66452:CDS
GCCGGAGGCGAAGGTTGCGT+TGG 0.541900 1.4:+4871947 MS.gene66452:CDS
TGCGAGCAATCGAATTTCCA+TGG 0.550132 1.4:-4871339 None:intergenic
GAAGATCGGAGACAAGTTGA+CGG 0.556676 1.4:-4871283 None:intergenic
GACCTGCAACTTCAAGAATA+CGG 0.556927 1.4:+4871440 MS.gene66452:CDS
AGAAGGTGGTGTGCCGGTGT+TGG 0.561302 1.4:+4871974 MS.gene66452:CDS
AGAACAGAAACAAGCTGCCA+TGG 0.562865 1.4:+4871322 MS.gene66452:CDS
GAATTTAACGCTCTAACTAA+CGG 0.564280 1.4:-4871536 None:intergenic
GGTGGAAGAAGGTGGTGTGC+CGG 0.565802 1.4:+4871968 MS.gene66452:CDS
TCGTCGGGAGGTAGAAGAAG+TGG 0.568427 1.4:-4871219 None:intergenic
AGAAACTAATTGAGCTTCTG+AGG 0.580867 1.4:+4873109 MS.gene66452:CDS
TCCACAATCTCAACCAACAC+CGG 0.586132 1.4:-4871987 None:intergenic
GTATACTCTGTGTTCTGTGA+AGG 0.596489 1.4:+4871806 MS.gene66452:CDS
CTAGGAAGTGGAGGAATACG+CGG 0.602566 1.4:+4871759 MS.gene66452:CDS
TAAGATGAGAGCGGTGAAAG+CGG 0.603828 1.4:+4871833 MS.gene66452:CDS
GGTTGGATCGCAGAGACAGA+AGG 0.604932 1.4:+4872010 MS.gene66452:CDS
GGCACCTTCACGAGCCACCA+TGG 0.605176 1.4:-4872087 None:intergenic
TATTCTGTTACAGATTTGTG+AGG 0.606208 1.4:+4872046 MS.gene66452:CDS
GTTGCGTTGGTGGAAGAAGG+TGG 0.609924 1.4:+4871960 MS.gene66452:CDS
ATTACAGGTGGAAGAGAACA+AGG 0.611067 1.4:+4871692 MS.gene66452:intron
AGTGTTGGTTGAGTTGATGG+CGG 0.612937 1.4:+4871866 MS.gene66452:CDS
TGCGTCAAAGCAACCAAAGG+AGG 0.612998 1.4:-4872113 None:intergenic
ACTGTTGCAGTCACTGAAAG+CGG 0.614710 1.4:-4871174 None:intergenic
GGCGAGGAAGAGTCGTCGGG+AGG 0.616350 1.4:-4871231 None:intergenic
GTGAATCTTCTAGGAAGTGG+AGG 0.623068 1.4:+4871750 MS.gene66452:CDS
AGATGAGAGCGGTGAAAGCG+GGG 0.626464 1.4:+4871835 MS.gene66452:CDS
GACTGCGTCAAAGCAACCAA+AGG 0.631800 1.4:-4872116 None:intergenic
AGCGGGGATAATGAAAGTGT+TGG 0.635236 1.4:+4871851 MS.gene66452:CDS
TAGGAAGTGGAGGAATACGC+GGG 0.637423 1.4:+4871760 MS.gene66452:CDS
TGTGTTGGTATCGGTGCCGG+AGG 0.656005 1.4:+4871932 MS.gene66452:CDS
AAGTTGACGGATGAGGTCAT+CGG 0.659006 1.4:-4871270 None:intergenic
TCGGGAGGTAGAAGAAGTGG+TGG 0.663074 1.4:-4871216 None:intergenic
GCAGAGACAGAAGGAAATTG+CGG 0.663880 1.4:+4872019 MS.gene66452:CDS
ACAGAGAGTTACACATACAT+GGG 0.672322 1.4:+4871107 MS.gene66452:CDS
TAATAACCTGAGATAGACGA+AGG 0.681280 1.4:-4871598 None:intergenic
ACAGATTTGTGAGGATAGCG+CGG 0.684005 1.4:+4872055 MS.gene66452:CDS
GTTGCAGTCACTGAAAGCGG+AGG 0.687507 1.4:-4871171 None:intergenic
CGCGGCGGTTCGATCCATGG+TGG 0.687911 1.4:+4872073 MS.gene66452:CDS
CAAAGAGAGATTCAAAATCG+CGG 0.688714 1.4:-4871468 None:intergenic
GGTGTTGGTTGAGATTGTGG+AGG 0.688844 1.4:+4871989 MS.gene66452:CDS
GAAGGAGAATAAGATGAGAG+CGG 0.703303 1.4:+4871824 MS.gene66452:CDS
GAGACAGAAGGAAATTGCGG+CGG 0.714686 1.4:+4872022 MS.gene66452:CDS
GATTTGTGAGGATAGCGCGG+CGG 0.801712 1.4:+4872058 MS.gene66452:CDS
ACAGGTGGAAGAGAACAAGG+CGG 0.815311 1.4:+4871695 MS.gene66452:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAATTAACTATATTTCAA+TGG + chr1.4:4872489-4872508 MS.gene66452:intron 10.0%
!! TAATAAATTAATAATAATGC+AGG - chr1.4:4872850-4872869 None:intergenic 10.0%
!!! TAATAATGTTTTTCTGTTTT+TGG + chr1.4:4873073-4873092 MS.gene66452:intron 15.0%
!!! TTTGTTTTCAATTTAATTAG+AGG + chr1.4:4872444-4872463 MS.gene66452:intron 15.0%
!! CTTGAATATTCTTATTGAAT+GGG + chr1.4:4872902-4872921 MS.gene66452:intron 20.0%
!! TCTTGAATATTCTTATTGAA+TGG + chr1.4:4872901-4872920 MS.gene66452:intron 20.0%
!!! ATAATATTCTACCTTTTGTT+TGG - chr1.4:4872156-4872175 None:intergenic 20.0%
!!! TCCTAAATTTTGTTTTTACT+CGG + chr1.4:4872304-4872323 MS.gene66452:intron 20.0%
!!! TTGATTATTGAAAGTTCTAA+CGG + chr1.4:4871664-4871683 MS.gene66452:intron 20.0%
! AAAAGAAGAGTTTCGATTTA+GGG - chr1.4:4872421-4872440 None:intergenic 25.0%
! ATTAGACACAATTAGACAAT+AGG - chr1.4:4872795-4872814 None:intergenic 25.0%
! GAAGTAACTAACGAAATTAA+TGG - chr1.4:4871416-4871435 None:intergenic 25.0%
! GACAACAACTAATAATTAAC+CGG - chr1.4:4871643-4871662 None:intergenic 25.0%
! GATGTAATAGTTAGTTATAC+CGG + chr1.4:4871621-4871640 MS.gene66452:intron 25.0%
! GCTAGTTCGATTTAAATTAT+TGG + chr1.4:4872326-4872345 MS.gene66452:intron 25.0%
! TTAGACACAATTAGACAATA+GGG - chr1.4:4872794-4872813 None:intergenic 25.0%
! TTTAAGAACTATTTATGTCG+TGG + chr1.4:4872936-4872955 MS.gene66452:intron 25.0%
!!! AATGTTTTTCTGTTTTTGGT+GGG + chr1.4:4873077-4873096 MS.gene66452:intron 25.0%
!!! TAATGTTTTTCTGTTTTTGG+TGG + chr1.4:4873076-4873095 MS.gene66452:intron 25.0%
AAAGAACTAATAGCTTCTTC+CGG + chr1.4:4871503-4871522 MS.gene66452:CDS 30.0%
AATTTCGTTAGTTACTTCAC+AGG + chr1.4:4871418-4871437 MS.gene66452:CDS 30.0%
GAAAAGAAGAGTTTCGATTT+AGG - chr1.4:4872422-4872441 None:intergenic 30.0%
GAATTTAACGCTCTAACTAA+CGG - chr1.4:4871539-4871558 None:intergenic 30.0%
GCCGAGTAAAAACAAAATTT+AGG - chr1.4:4872308-4872327 None:intergenic 30.0%
TATTCTGTTACAGATTTGTG+AGG + chr1.4:4872046-4872065 MS.gene66452:CDS 30.0%
TTCATTATTGTCCAAACATG+TGG - chr1.4:4873009-4873028 None:intergenic 30.0%
TTTCTGTTCTTCGATTGAAT+CGG - chr1.4:4871312-4871331 None:intergenic 30.0%
! TTAGTTAGAGCGTTAAATTC+AGG + chr1.4:4871539-4871558 MS.gene66452:CDS 30.0%
!! ATCGAATATGGTGGATAAAT+CGG + chr1.4:4871896-4871915 MS.gene66452:CDS 30.0%
!!! GCAATTTTGATTCGATTTTC+CGG - chr1.4:4871374-4871393 None:intergenic 30.0%
AACAGAGAGTTACACATACA+TGG + chr1.4:4871106-4871125 MS.gene66452:CDS 35.0%
AACTAACGGTTTAATAGCTC+CGG - chr1.4:4871525-4871544 None:intergenic 35.0%
ACAGAGAGTTACACATACAT+GGG + chr1.4:4871107-4871126 MS.gene66452:CDS 35.0%
AGAAACTAATTGAGCTTCTG+AGG + chr1.4:4873109-4873128 MS.gene66452:CDS 35.0%
AGACAATAGGGCAAAAATTG+CGG - chr1.4:4872782-4872801 None:intergenic 35.0%
ATTGCTCGCAAAGAATAAAC+CGG + chr1.4:4871352-4871371 MS.gene66452:CDS 35.0%
CAAAGAGAGATTCAAAATCG+CGG - chr1.4:4871471-4871490 None:intergenic 35.0%
CCTAGAAGATTCACCAATAA+CGG - chr1.4:4871744-4871763 None:intergenic 35.0%
CGAATCAAAATTGCTAAAGC+TGG + chr1.4:4871380-4871399 MS.gene66452:CDS 35.0%
GGTTCGAATTCTTCATTGAT+TGG + chr1.4:4872510-4872529 MS.gene66452:intron 35.0%
GTTCTAACGGTAATGATTAC+AGG + chr1.4:4871677-4871696 MS.gene66452:intron 35.0%
TAATAACCTGAGATAGACGA+AGG - chr1.4:4871601-4871620 None:intergenic 35.0%
TCTTCCACCAAAGCAATAAA+CGG - chr1.4:4872708-4872727 None:intergenic 35.0%
! GGATTTTGAATCGAATATGG+TGG + chr1.4:4871887-4871906 MS.gene66452:CDS 35.0%
!! TGTTTTGAGTGTGTTGGTAT+CGG + chr1.4:4871923-4871942 MS.gene66452:CDS 35.0%
AATAGGGCAAAAATTGCGGT+GGG - chr1.4:4872778-4872797 None:intergenic 40.0%
ATCAGGTGCAATTCCGTTAT+TGG + chr1.4:4871728-4871747 MS.gene66452:CDS 40.0%
ATTACAGGTGGAAGAGAACA+AGG + chr1.4:4871692-4871711 MS.gene66452:intron 40.0%
CCGTTATTGGTGAATCTTCT+AGG + chr1.4:4871741-4871760 MS.gene66452:CDS 40.0%
CTAACGGTAATGATTACAGG+TGG + chr1.4:4871680-4871699 MS.gene66452:intron 40.0%
CTATAGTAGTGCCACATGTT+TGG + chr1.4:4872995-4873014 MS.gene66452:intron 40.0%
GAAGGAGAATAAGATGAGAG+CGG + chr1.4:4871824-4871843 MS.gene66452:CDS 40.0%
GACCTGCAACTTCAAGAATA+CGG + chr1.4:4871440-4871459 MS.gene66452:CDS 40.0%
GATTGAATCGGAGTGAAGAT+CGG - chr1.4:4871300-4871319 None:intergenic 40.0%
GTATACTCTGTGTTCTGTGA+AGG + chr1.4:4871806-4871825 MS.gene66452:CDS 40.0%
TCTGATGTACGTGTAGATCT+TGG - chr1.4:4873138-4873157 None:intergenic 40.0%
TTTGTCGTATGAACGCAATG+AGG - chr1.4:4872827-4872846 None:intergenic 40.0%
! GATGAGGTCATCGGAATTTT+CGG - chr1.4:4871264-4871283 None:intergenic 40.0%
! TTGGTGAATCTTCTAGGAAG+TGG + chr1.4:4871747-4871766 MS.gene66452:CDS 40.0%
!! GAAAGTGTTGGTTGAGTTGA+TGG + chr1.4:4871863-4871882 MS.gene66452:CDS 40.0%
!! GGCGGATTTTGAATCGAATA+TGG + chr1.4:4871884-4871903 MS.gene66452:CDS 40.0%
!! GGCTTATGTTTTGAGTGTGT+TGG + chr1.4:4871917-4871936 MS.gene66452:CDS 40.0%
!!! TTTCTGTTTTTGGTGGGTTC+AGG + chr1.4:4873083-4873102 MS.gene66452:intron 40.0%
ACAGATTTGTGAGGATAGCG+CGG + chr1.4:4872055-4872074 MS.gene66452:CDS 45.0%
ACTGTTGCAGTCACTGAAAG+CGG - chr1.4:4871177-4871196 None:intergenic 45.0%
AGAACAGAAACAAGCTGCCA+TGG + chr1.4:4871322-4871341 MS.gene66452:CDS 45.0%
CAAAGCAACCAAAGGAGGAA+TGG - chr1.4:4872111-4872130 None:intergenic 45.0%
CAATAGGGCAAAAATTGCGG+TGG - chr1.4:4872779-4872798 None:intergenic 45.0%
CACAAATCGCGCCAAACAAA+AGG + chr1.4:4872142-4872161 MS.gene66452:CDS 45.0%
CACCGTATTCTTGAAGTTGC+AGG - chr1.4:4871445-4871464 None:intergenic 45.0%
GCAGAGACAGAAGGAAATTG+CGG + chr1.4:4872019-4872038 MS.gene66452:CDS 45.0%
GGAAGTTGAATCGTCGTTGA+TGG - chr1.4:4871153-4871172 None:intergenic 45.0%
TAAGATGAGAGCGGTGAAAG+CGG + chr1.4:4871833-4871852 MS.gene66452:CDS 45.0%
TCCACAATCTCAACCAACAC+CGG - chr1.4:4871990-4872009 None:intergenic 45.0%
TGCGAGCAATCGAATTTCCA+TGG - chr1.4:4871342-4871361 None:intergenic 45.0%
TGTCGTGGAAGCTTATCATG+CGG + chr1.4:4872951-4872970 MS.gene66452:intron 45.0%
TTGGTTGAGATTGTGGAGGT+TGG + chr1.4:4871993-4872012 MS.gene66452:CDS 45.0%
! AAGTTGACGGATGAGGTCAT+CGG - chr1.4:4871273-4871292 None:intergenic 45.0%
! AGCGGGGATAATGAAAGTGT+TGG + chr1.4:4871851-4871870 MS.gene66452:CDS 45.0%
! GAAGATCGGAGACAAGTTGA+CGG - chr1.4:4871286-4871305 None:intergenic 45.0%
! GTGAATCTTCTAGGAAGTGG+AGG + chr1.4:4871750-4871769 MS.gene66452:CDS 45.0%
!! AGTGTTGGTTGAGTTGATGG+CGG + chr1.4:4871866-4871885 MS.gene66452:CDS 45.0%
!! CATGGTGCCGTTTATTGCTT+TGG + chr1.4:4872698-4872717 MS.gene66452:intron 45.0%
!! ATTTAATATTTAAACTAAAA+TGG - chr1.4:4872623-4872642 None:intergenic 5.0%
AAGATGAGAGCGGTGAAAGC+GGG + chr1.4:4871834-4871853 MS.gene66452:CDS 50.0%
ACAGGTGGAAGAGAACAAGG+CGG + chr1.4:4871695-4871714 MS.gene66452:intron 50.0%
AGCATTGCTCCCTCAACTTG+CGG + chr1.4:4872663-4872682 MS.gene66452:intron 50.0%
CTAGGAAGTGGAGGAATACG+CGG + chr1.4:4871759-4871778 MS.gene66452:CDS 50.0%
GACTGCAACAGTGACAGATC+CGG + chr1.4:4871185-4871204 MS.gene66452:CDS 50.0%
GACTGCGTCAAAGCAACCAA+AGG - chr1.4:4872119-4872138 None:intergenic 50.0%
GAGACAGAAGGAAATTGCGG+CGG + chr1.4:4872022-4872041 MS.gene66452:CDS 50.0%
GCAAGAACAATGAGTCGACG+TGG - chr1.4:4872741-4872760 None:intergenic 50.0%
TAGGAAGTGGAGGAATACGC+GGG + chr1.4:4871760-4871779 MS.gene66452:CDS 50.0%
TGCGTCAAAGCAACCAAAGG+AGG - chr1.4:4872116-4872135 None:intergenic 50.0%
TGCTCTCCTTCGTCTATCTC+AGG + chr1.4:4871592-4871611 MS.gene66452:CDS 50.0%
TTGTTTGGCGCGATTTGTGC+CGG - chr1.4:4872141-4872160 None:intergenic 50.0%
! AAGGTTGCGTTGGTGGAAGA+AGG + chr1.4:4871957-4871976 MS.gene66452:CDS 50.0%
! TTGGTTGCTTTGACGCAGTC+CGG + chr1.4:4872119-4872138 MS.gene66452:CDS 50.0%
!! ATCGGAATTTTCGGAAGCGC+AGG - chr1.4:4871255-4871274 None:intergenic 50.0%
!! GGTGCCGTTTATTGCTTTGG+TGG + chr1.4:4872701-4872720 MS.gene66452:intron 50.0%
!! GGTGTTGGTTGAGATTGTGG+AGG + chr1.4:4871989-4872008 MS.gene66452:CDS 50.0%
!! TGAAGGTGCCATTCCTCCTT+TGG + chr1.4:4872100-4872119 MS.gene66452:CDS 50.0%
AAGGCGGCGATTGGAAGATC+AGG + chr1.4:4871711-4871730 MS.gene66452:CDS 55.0%
ACGTCAGCTACCGCAAGTTG+AGG - chr1.4:4872676-4872695 None:intergenic 55.0%
AGATGAGAGCGGTGAAAGCG+GGG + chr1.4:4871835-4871854 MS.gene66452:CDS 55.0%
AGGGCAAAAATTGCGGTGGG+TGG - chr1.4:4872775-4872794 None:intergenic 55.0%
GAAGAGAACAAGGCGGCGAT+TGG + chr1.4:4871702-4871721 MS.gene66452:CDS 55.0%
GATTTGTGAGGATAGCGCGG+CGG + chr1.4:4872058-4872077 MS.gene66452:CDS 55.0%
GCAAAAATTGCGGTGGGTGG+AGG - chr1.4:4872772-4872791 None:intergenic 55.0%
GGTTGGATCGCAGAGACAGA+AGG + chr1.4:4872010-4872029 MS.gene66452:CDS 55.0%
GTTGCAGTCACTGAAAGCGG+AGG - chr1.4:4871174-4871193 None:intergenic 55.0%
TCGGGAGGTAGAAGAAGTGG+TGG - chr1.4:4871219-4871238 None:intergenic 55.0%
TCGTCGGGAGGTAGAAGAAG+TGG - chr1.4:4871222-4871241 None:intergenic 55.0%
TGGAGGAATACGCGGGAAGA+AGG + chr1.4:4871767-4871786 MS.gene66452:CDS 55.0%
TTGCGGTAGCTGACGTTGCA+TGG + chr1.4:4872680-4872699 MS.gene66452:intron 55.0%
! AGAAGTGGTGGCGAATTCGC+CGG - chr1.4:4871207-4871226 None:intergenic 55.0%
! CGGAGACAAGTTGACGGATG+AGG - chr1.4:4871280-4871299 None:intergenic 55.0%
! CGTCAGCTACCGCAAGTTGA+GGG - chr1.4:4872675-4872694 None:intergenic 55.0%
! GTTGCGTTGGTGGAAGAAGG+TGG + chr1.4:4871960-4871979 MS.gene66452:CDS 55.0%
!! AATTTTCGGAAGCGCAGGCG+AGG - chr1.4:4871250-4871269 None:intergenic 55.0%
!! GAGTGTGTTGGTATCGGTGC+CGG + chr1.4:4871929-4871948 MS.gene66452:CDS 55.0%
!! GCCGGTGTTGGTTGAGATTG+TGG + chr1.4:4871986-4872005 MS.gene66452:CDS 55.0%
ACCAACGCAACCTTCGCCTC+CGG - chr1.4:4871951-4871970 None:intergenic 60.0%
CGATCCATGGTGGCTCGTGA+AGG + chr1.4:4872083-4872102 MS.gene66452:CDS 60.0%
CGGGAAGAAGGACGCGTCTA+CGG + chr1.4:4871779-4871798 MS.gene66452:CDS 60.0%
GGTGGAAGAAGGTGGTGTGC+CGG + chr1.4:4871968-4871987 MS.gene66452:CDS 60.0%
! AGAAGGTGGTGTGCCGGTGT+TGG + chr1.4:4871974-4871993 MS.gene66452:CDS 60.0%
!! TGTGTTGGTATCGGTGCCGG+AGG + chr1.4:4871932-4871951 MS.gene66452:CDS 60.0%
CGCAGGCGAGGAAGAGTCGT+CGG - chr1.4:4871238-4871257 None:intergenic 65.0%
GCAGGCGAGGAAGAGTCGTC+GGG - chr1.4:4871237-4871256 None:intergenic 65.0%
GGAGGCGAAGGTTGCGTTGG+TGG + chr1.4:4871950-4871969 MS.gene66452:CDS 65.0%
GGCACCTTCACGAGCCACCA+TGG - chr1.4:4872090-4872109 None:intergenic 65.0%
TAGCGCGGCGGTTCGATCCA+TGG + chr1.4:4872070-4872089 MS.gene66452:CDS 65.0%
CGCGGCGGTTCGATCCATGG+TGG + chr1.4:4872073-4872092 MS.gene66452:CDS 70.0%
GCCGGAGGCGAAGGTTGCGT+TGG + chr1.4:4871947-4871966 MS.gene66452:CDS 70.0%
GGCGAGGAAGAGTCGTCGGG+AGG - chr1.4:4871234-4871253 None:intergenic 70.0%
!! GGTATCGGTGCCGGAGGCGA+AGG + chr1.4:4871938-4871957 MS.gene66452:CDS 70.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 4871101 4873161 4871101 ID=MS.gene66452
chr1.4 mRNA 4871101 4873161 4871101 ID=MS.gene66452.t1;Parent=MS.gene66452
chr1.4 exon 4871101 4871613 4871101 ID=MS.gene66452.t1.exon1;Parent=MS.gene66452.t1
chr1.4 CDS 4871101 4871613 4871101 ID=cds.MS.gene66452.t1;Parent=MS.gene66452.t1
chr1.4 exon 4871699 4872163 4871699 ID=MS.gene66452.t1.exon2;Parent=MS.gene66452.t1
chr1.4 CDS 4871699 4872163 4871699 ID=cds.MS.gene66452.t1;Parent=MS.gene66452.t1
chr1.4 exon 4873105 4873161 4873105 ID=MS.gene66452.t1.exon3;Parent=MS.gene66452.t1
chr1.4 CDS 4873105 4873161 4873105 ID=cds.MS.gene66452.t1;Parent=MS.gene66452.t1
Gene Sequence

>MS.gene66452

ATGGGAACAGAGAGTTACACATACATGGGACGAAGTTTCAAAGAATTCACCATCAACGACGATTCAACTTCCTCCGCTTTCAGTGACTGCAACAGTGACAGATCCGGCGAATTCGCCACCACTTCTTCTACCTCCCGACGACTCTTCCTCGCCTGCGCTTCCGAAAATTCCGATGACCTCATCCGTCAACTTGTCTCCGATCTTCACTCCGATTCAATCGAAGAACAGAAACAAGCTGCCATGGAAATTCGATTGCTCGCAAAGAATAAACCGGAAAATCGAATCAAAATTGCTAAAGCTGGCGCGATTAAACCATTAATTTCGTTAGTTACTTCACAGGACCTGCAACTTCAAGAATACGGTGTTACCGCGATTTTGAATCTCTCTTTGTGTGACGAGAATAAAGAACTAATAGCTTCTTCCGGAGCTATTAAACCGTTAGTTAGAGCGTTAAATTCAGGAACATCAACAGCTAAAGAGAACGCTGCTTGTGCTCTCCTTCGTCTATCTCAGGTTATTAGATGTAATAGTTAGTTATACCGGTTAATTATTAGTTGTTGTCATTGATTATTGAAAGTTCTAACGGTAATGATTACAGGTGGAAGAGAACAAGGCGGCGATTGGAAGATCAGGTGCAATTCCGTTATTGGTGAATCTTCTAGGAAGTGGAGGAATACGCGGGAAGAAGGACGCGTCTACGGCGCTGTATACTCTGTGTTCTGTGAAGGAGAATAAGATGAGAGCGGTGAAAGCGGGGATAATGAAAGTGTTGGTTGAGTTGATGGCGGATTTTGAATCGAATATGGTGGATAAATCGGCTTATGTTTTGAGTGTGTTGGTATCGGTGCCGGAGGCGAAGGTTGCGTTGGTGGAAGAAGGTGGTGTGCCGGTGTTGGTTGAGATTGTGGAGGTTGGATCGCAGAGACAGAAGGAAATTGCGGCGGTTATTCTGTTACAGATTTGTGAGGATAGCGCGGCGGTTCGATCCATGGTGGCTCGTGAAGGTGCCATTCCTCCTTTGGTTGCTTTGACGCAGTCCGGCACAAATCGCGCCAAACAAAAGGTAGAATATTATTATCTTTACTTTTTTTTTATTAATTATATATTGATAATTTGATATAGATGTTAATTATTCAATAGCGTAATTTTTCAAAAAAAAATTATTCAATAATCTAATTTTCGAATTAGATGACTCGAGTCTTTTCCTAAATTTTGTTTTTACTCGGCTAGTTCGATTTAAATTATTGGCTCAAATTTAGTCTTGACTTATCTCTTTCAGCGTAACTAAATGTTCAAATGTCGTTAGTTTGAACTCAGCCCTAAATCGAAACTCTTCTTTTCTATTTGTTTTCAATTTAATTAGAGGTCATCAATTCAAATTTAGTACAATTAATTAACTATATTTCAATGGTTCGAATTCTTCATTGATTGGTTCGAATGTAATTTTGATCTTTTTTTTTAACAAAAATGTAATTTTGATCTTGAAAATGCAGCAGCATAAAAAAAATTACACACATTGCCATTTTAGTTTAAATATTAAATGAATTATTCAGCTTGAATTCAGCATTGCTCCCTCAACTTGCGGTAGCTGACGTTGCATGGTGCCGTTTATTGCTTTGGTGGAAGAACATGTAATGCCACGTCGACTCATTGTTCTTGCAGAATCGACCTCCACCCACCGCAATTTTTGCCCTATTGTCTAATTGTGTCTAATTAAAATGCTCCTCATTGCGTTCATACGACAAACCTGCATTATTATTAATTTATTATTATTATCATGTAGTAATATATTTCGTAAAATCTTGAATATTCTTATTGAATGGGTATAGCATTATTTTAAGAACTATTTATGTCGTGGAAGCTTATCATGCGGCTTGTGTTGTGCTTTTTTTTTCTATAGTAGTGCCACATGTTTGGACAATAATGAAATTTAGAAATCACGTTAATATCTCAATCATTTCATTTTTTTTAATAATAATGTTTTTCTGTTTTTGGTGGGTTCAGGCTGAGAAACTAATTGAGCTTCTGAGGCAACCAAGATCTACACGTACATCAGAATGA
Protein sequence

>MS.gene66452.t1

MGTESYTYMGRSFKEFTINDDSTSSAFSDCNSDRSGEFATTSSTSRRLFLACASENSDDLIRQLVSDLHSDSIEEQKQAAMEIRLLAKNKPENRIKIAKAGAIKPLISLVTSQDLQLQEYGVTAILNLSLCDENKELIASSGAIKPLVRALNSGTSTAKENAACALLRLSQVEENKAAIGRSGAIPLLVNLLGSGGIRGKKDASTALYTLCSVKENKMRAVKAGIMKVLVELMADFESNMVDKSAYVLSVLVSVPEAKVALVEEGGVPVLVEIVEVGSQRQKEIAAVILLQICEDSAAVRSMVAREGAIPPLVALTQSGTNRAKQKAEKLIELLRQPRSTRTSE