Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053189.t1 | ACJ83936.1 | 86.2 | 159 | 8 | 3 | 1 | 145 | 1 | 159 | 2.20E-69 | 271.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053189.t1 | Q9SAG8 | 39.1 | 133 | 67 | 3 | 23 | 144 | 33 | 162 | 8.2e-17 | 88.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053189.t1 | B7FGQ7 | 86.2 | 159 | 8 | 3 | 1 | 145 | 1 | 159 | 1.6e-69 | 271.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050288 | MS.gene053189 | 0.802968 | 4.36E-49 | -1.69E-46 |
| MS.gene053045 | MS.gene053189 | 0.811161 | 8.04E-51 | -1.69E-46 |
| MS.gene053188 | MS.gene053189 | 0.800647 | 1.31E-48 | -1.69E-46 |
| MS.gene053189 | MS.gene054222 | 0.803729 | 3.03E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene054536 | 0.887276 | 1.64E-72 | -1.69E-46 |
| MS.gene053189 | MS.gene05469 | 0.834404 | 3.06E-56 | -1.69E-46 |
| MS.gene053189 | MS.gene05795 | 0.80522 | 1.48E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene060840 | 0.802967 | 4.36E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene062501 | 0.800542 | 1.37E-48 | -1.69E-46 |
| MS.gene053189 | MS.gene063245 | 0.810657 | 1.03E-50 | -1.69E-46 |
| MS.gene053189 | MS.gene063247 | 0.805363 | 1.38E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene06441 | 0.812395 | 4.33E-51 | -1.69E-46 |
| MS.gene053189 | MS.gene066718 | 0.804548 | 2.05E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene067981 | 0.808081 | 3.69E-50 | -1.69E-46 |
| MS.gene053189 | MS.gene068565 | 0.836298 | 1.02E-56 | -1.69E-46 |
| MS.gene053189 | MS.gene071975 | 0.800541 | 1.37E-48 | -1.69E-46 |
| MS.gene053189 | MS.gene072970 | 0.811531 | 6.68E-51 | -1.69E-46 |
| MS.gene053189 | MS.gene09850 | 0.819026 | 1.44E-52 | -1.69E-46 |
| MS.gene053189 | MS.gene20064 | 0.843801 | 1.12E-58 | -1.69E-46 |
| MS.gene053189 | MS.gene21659 | 0.806644 | 7.44E-50 | -1.69E-46 |
| MS.gene053189 | MS.gene22314 | 0.815998 | 6.94E-52 | -1.69E-46 |
| MS.gene053189 | MS.gene22433 | 0.846305 | 2.36E-59 | -1.69E-46 |
| MS.gene053189 | MS.gene24413 | 0.805499 | 1.30E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene28491 | 0.802345 | 5.86E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene29794 | 0.809044 | 2.30E-50 | -1.69E-46 |
| MS.gene053189 | MS.gene34444 | 0.810972 | 8.84E-51 | -1.69E-46 |
| MS.gene053189 | MS.gene36037 | 0.802062 | 6.71E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene39190 | 0.835975 | 1.23E-56 | -1.69E-46 |
| MS.gene053189 | MS.gene40908 | 0.803305 | 3.71E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene41775 | 0.804734 | 1.87E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene46301 | 0.808528 | 2.96E-50 | -1.69E-46 |
| MS.gene053189 | MS.gene46355 | 0.80434 | 2.26E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene46390 | 0.840813 | 6.94E-58 | -1.69E-46 |
| MS.gene053189 | MS.gene50407 | 0.805066 | 1.60E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene58467 | 0.814171 | 1.76E-51 | -1.69E-46 |
| MS.gene053189 | MS.gene59540 | 0.805017 | 1.63E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene60300 | 0.801908 | 7.21E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene60384 | 0.802742 | 4.86E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene61162 | 0.811514 | 6.74E-51 | -1.69E-46 |
| MS.gene053189 | MS.gene61254 | 0.839355 | 1.66E-57 | -1.69E-46 |
| MS.gene053189 | MS.gene62676 | 0.804731 | 1.88E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene66408 | 0.881993 | 1.51E-70 | -1.69E-46 |
| MS.gene053189 | MS.gene66452 | 0.834419 | 3.03E-56 | -1.69E-46 |
| MS.gene053189 | MS.gene66960 | 0.813192 | 2.90E-51 | -1.69E-46 |
| MS.gene053189 | MS.gene67136 | 0.831793 | 1.36E-55 | -1.69E-46 |
| MS.gene053189 | MS.gene72379 | 0.828754 | 7.54E-55 | -1.69E-46 |
| MS.gene053189 | MS.gene72620 | 0.808192 | 3.49E-50 | -1.69E-46 |
| MS.gene053189 | MS.gene73270 | 0.825836 | 3.77E-54 | -1.69E-46 |
| MS.gene053189 | MS.gene74530 | 0.803844 | 2.87E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene74972 | 0.801407 | 9.14E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene74982 | 0.81708 | 3.97E-52 | -1.69E-46 |
| MS.gene053189 | MS.gene74985 | 0.802633 | 5.11E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene78773 | 0.809241 | 2.09E-50 | -1.69E-46 |
| MS.gene053189 | MS.gene88351 | 0.801603 | 8.33E-49 | -1.69E-46 |
| MS.gene053189 | MS.gene99848 | 0.800426 | 1.45E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053189.t1 | MTR_4g007300 | 85.714 | 161 | 7 | 3 | 1 | 145 | 1 | 161 | 9.91e-95 | 270 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053189.t1 | AT1G80920 | 55.455 | 110 | 37 | 2 | 46 | 144 | 54 | 162 | 4.28e-38 | 127 |
Find 37 sgRNAs with CRISPR-Local
Find 66 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTACTGCTGTTGGTGTTAT+TGG | 0.156544 | 4.2:+92291371 | MS.gene053189:CDS |
| ATGATCCAGATTGGGGCTAT+TGG | 0.284504 | 4.2:+92292401 | MS.gene053189:CDS |
| TGAATTCTCAAAGTCTTATA+AGG | 0.285846 | 4.2:-92291503 | None:intergenic |
| AAAATAAGATGAATAAAGTT+AGG | 0.311546 | 4.2:+92291441 | MS.gene053189:CDS |
| CATAAACTTTATTAAATGTA+AGG | 0.334186 | 4.2:-92292481 | None:intergenic |
| GTAATGGATCTTGTGTTTCA+TGG | 0.354259 | 4.2:+92291396 | MS.gene053189:CDS |
| TCTCTTCCTTTGCACACATC+TGG | 0.358215 | 4.2:-92291744 | None:intergenic |
| TGATCCAGATTGGGGCTATT+GGG | 0.375755 | 4.2:+92292402 | MS.gene053189:CDS |
| GTTGGTGTTATTGGTGGTAA+TGG | 0.380315 | 4.2:+92291380 | MS.gene053189:CDS |
| GACAATGATGAGTCATTTAG+AGG | 0.388181 | 4.2:+92292373 | MS.gene053189:CDS |
| TGTTTCATGGATTGGAAGAA+AGG | 0.400791 | 4.2:+92291409 | MS.gene053189:CDS |
| TCTTCCCAATAGCCCCAATC+TGG | 0.418268 | 4.2:-92292406 | None:intergenic |
| GGGCTATTGGGAAGAATGGA+TGG | 0.423463 | 4.2:+92292414 | MS.gene053189:CDS |
| GAGGAATGAATGATCCAGAT+TGG | 0.426354 | 4.2:+92292392 | MS.gene053189:CDS |
| AGGAATGAATGATCCAGATT+GGG | 0.438090 | 4.2:+92292393 | MS.gene053189:CDS |
| ATCATTGTCTTCATTATATG+TGG | 0.460660 | 4.2:-92292359 | None:intergenic |
| GGATCTTGTGTTTCATGGAT+TGG | 0.460943 | 4.2:+92291401 | MS.gene053189:CDS |
| ATTGGGGCTATTGGGAAGAA+TGG | 0.464008 | 4.2:+92292410 | MS.gene053189:CDS |
| AAATAAGATGAATAAAGTTA+GGG | 0.476392 | 4.2:+92291442 | MS.gene053189:CDS |
| ATCTAAATCTTCTTCTGTGA+TGG | 0.478827 | 4.2:+92291478 | MS.gene053189:CDS |
| TTGGGAAGAATGGATGGGTT+GGG | 0.486704 | 4.2:+92292420 | MS.gene053189:CDS |
| ATTGGGAAGAATGGATGGGT+TGG | 0.503999 | 4.2:+92292419 | MS.gene053189:CDS |
| TTCCTTTGCACACATCTGGA+TGG | 0.515608 | 4.2:-92291740 | None:intergenic |
| ACTGCTGTTGGTGTTATTGG+TGG | 0.520448 | 4.2:+92291374 | MS.gene053189:CDS |
| GGCTATTGGGAAGAATGGAT+GGG | 0.528910 | 4.2:+92292415 | MS.gene053189:CDS |
| GTGTGCAAAGGAAGAGATTG+TGG | 0.529500 | 4.2:+92291750 | MS.gene053189:CDS |
| GAAGAATGGATGGGTTGGGA+AGG | 0.548816 | 4.2:+92292424 | MS.gene053189:CDS |
| TGGATGGGTTGGGAAGGAGC+AGG | 0.559751 | 4.2:+92292430 | MS.gene053189:CDS |
| TGTGCAAAGGAAGAGATTGT+GGG | 0.575497 | 4.2:+92291751 | MS.gene053189:CDS |
| GCAGTTTCACTTAATCAATG+AGG | 0.585631 | 4.2:+92291776 | MS.gene053189:CDS |
| ATGGCTGCTACTACTGCTGT+TGG | 0.598684 | 4.2:+92291362 | MS.gene053189:CDS |
| TACCATCCAGATGTGTGCAA+AGG | 0.620033 | 4.2:+92291738 | MS.gene053189:CDS |
| TAATATGAGAAGAGTAGTCA+CGG | 0.620194 | 4.2:-92292456 | None:intergenic |
| GGAATGAATGATCCAGATTG+GGG | 0.639140 | 4.2:+92292394 | MS.gene053189:CDS |
| ACATCAGATTCAGAAGCACC+AGG | 0.654245 | 4.2:-92291527 | None:intergenic |
| AAGACTTTGAGAATTCAACC+TGG | 0.677099 | 4.2:+92291509 | MS.gene053189:CDS |
| GTGCAAAGGAAGAGATTGTG+GGG | 0.740567 | 4.2:+92291752 | MS.gene053189:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTTATTGTTATAATTGTT+TGG | + | chr4.2:92291860-92291879 | MS.gene053189:intron | 10.0% |
| !! | ATGTATTAATATTGAATATT+TGG | + | chr4.2:92291696-92291715 | MS.gene053189:intron | 10.0% |
| !! | ATTTATTGTTATAATTGTTT+GGG | + | chr4.2:92291861-92291880 | MS.gene053189:intron | 10.0% |
| !! | TGTATTAATATTGAATATTT+GGG | + | chr4.2:92291697-92291716 | MS.gene053189:intron | 10.0% |
| !!! | TTTTAGATCATAAAAATATA+TGG | + | chr4.2:92291895-92291914 | MS.gene053189:intron | 10.0% |
| !! | AAAATAAGATGAATAAAGTT+AGG | + | chr4.2:92291441-92291460 | MS.gene053189:CDS | 15.0% |
| !! | AAACAAATCATTAAACTATA+TGG | - | chr4.2:92292224-92292243 | None:intergenic | 15.0% |
| !! | AAATAAGATGAATAAAGTTA+GGG | + | chr4.2:92291442-92291461 | MS.gene053189:CDS | 15.0% |
| !! | CATAAATTTCAATTAGATAA+TGG | - | chr4.2:92292266-92292285 | None:intergenic | 15.0% |
| !!! | AATTTGTTTTTATCTTCTTA+TGG | + | chr4.2:92292196-92292215 | MS.gene053189:intron | 15.0% |
| !!! | ATTTGTTTTTATCTTCTTAT+GGG | + | chr4.2:92292197-92292216 | MS.gene053189:intron | 15.0% |
| !!! | TTTTTTATTGTTTATGGTTT+TGG | + | chr4.2:92292063-92292082 | MS.gene053189:intron | 15.0% |
| !! | AACTATGCAATGAAAATTAA+AGG | - | chr4.2:92291840-92291859 | None:intergenic | 20.0% |
| !! | ACAATAAAAAAAACTGGATT+TGG | - | chr4.2:92292054-92292073 | None:intergenic | 20.0% |
| !! | CCATAAACAATAAAAAAAAC+TGG | - | chr4.2:92292060-92292079 | None:intergenic | 20.0% |
| !! | GTTTATCAAGTTATAAATCT+TGG | - | chr4.2:92292100-92292119 | None:intergenic | 20.0% |
| !!! | AATTTATGTGTTGCTTATAT+TGG | + | chr4.2:92292278-92292297 | MS.gene053189:intron | 20.0% |
| !!! | ACTAATTTGATTGATGATTT+TGG | + | chr4.2:92291607-92291626 | MS.gene053189:intron | 20.0% |
| !!! | CCAGTTTTTTTTATTGTTTA+TGG | + | chr4.2:92292057-92292076 | MS.gene053189:intron | 20.0% |
| !!! | GTTGTTTTATTTCAAATCAA+TGG | - | chr4.2:92291996-92292015 | None:intergenic | 20.0% |
| !!! | TTTTACATTTTCTCTCAAAT+TGG | - | chr4.2:92292323-92292342 | None:intergenic | 20.0% |
| ! | ATCATTGTCTTCATTATATG+TGG | - | chr4.2:92292362-92292381 | None:intergenic | 25.0% |
| ! | ATTGGAGATATAAGATCAAT+GGG | + | chr4.2:92291930-92291949 | MS.gene053189:intron | 25.0% |
| ! | CATTATACATGAAGAAAGAA+AGG | - | chr4.2:92291679-92291698 | None:intergenic | 25.0% |
| !! | ATATGGATATTGATTGACAT+TGG | + | chr4.2:92291912-92291931 | MS.gene053189:intron | 25.0% |
| !! | TGAATTCTCAAAGTCTTATA+AGG | - | chr4.2:92291506-92291525 | None:intergenic | 25.0% |
| !! | TGGATTTGTAATCTTGATTT+TGG | + | chr4.2:92291627-92291646 | MS.gene053189:intron | 25.0% |
| ATCTAAATCTTCTTCTGTGA+TGG | + | chr4.2:92291478-92291497 | MS.gene053189:CDS | 30.0% | |
| CATTGGAGATATAAGATCAA+TGG | + | chr4.2:92291929-92291948 | MS.gene053189:intron | 30.0% | |
| TAATATGAGAAGAGTAGTCA+CGG | - | chr4.2:92292459-92292478 | None:intergenic | 30.0% | |
| TGTGACACAATTATTGATCT+TGG | - | chr4.2:92292018-92292037 | None:intergenic | 30.0% | |
| TTCTGAATCTGATGTTAGAA+AGG | + | chr4.2:92291535-92291554 | MS.gene053189:intron | 30.0% | |
| ! | GGATAGCTTAGAGAAATTTT+AGG | - | chr4.2:92291819-92291838 | None:intergenic | 30.0% |
| AGGAATGAATGATCCAGATT+GGG | + | chr4.2:92292393-92292412 | MS.gene053189:CDS | 35.0% | |
| CTTCTCATCTATGGTTTCAT+TGG | - | chr4.2:92292166-92292185 | None:intergenic | 35.0% | |
| GACAATGATGAGTCATTTAG+AGG | + | chr4.2:92292373-92292392 | MS.gene053189:CDS | 35.0% | |
| GCAGTTTCACTTAATCAATG+AGG | + | chr4.2:92291776-92291795 | MS.gene053189:CDS | 35.0% | |
| ! | AAGACTTTGAGAATTCAACC+TGG | + | chr4.2:92291509-92291528 | MS.gene053189:CDS | 35.0% |
| !! | CTTTTGTTGCTTCTCATCTA+TGG | - | chr4.2:92292175-92292194 | None:intergenic | 35.0% |
| !! | GTAATGGATCTTGTGTTTCA+TGG | + | chr4.2:92291396-92291415 | MS.gene053189:CDS | 35.0% |
| !! | TGTTTCATGGATTGGAAGAA+AGG | + | chr4.2:92291409-92291428 | MS.gene053189:CDS | 35.0% |
| !! | TTTTAGACAACTTGCTTTGC+AGG | + | chr4.2:92291559-92291578 | MS.gene053189:intron | 35.0% |
| GAGGAATGAATGATCCAGAT+TGG | + | chr4.2:92292392-92292411 | MS.gene053189:CDS | 40.0% | |
| GGAATGAATGATCCAGATTG+GGG | + | chr4.2:92292394-92292413 | MS.gene053189:CDS | 40.0% | |
| GGATCTTGTGTTTCATGGAT+TGG | + | chr4.2:92291401-92291420 | MS.gene053189:CDS | 40.0% | |
| TGTGCAAAGGAAGAGATTGT+GGG | + | chr4.2:92291751-92291770 | MS.gene053189:CDS | 40.0% | |
| !! | ACTACTGCTGTTGGTGTTAT+TGG | + | chr4.2:92291371-92291390 | MS.gene053189:CDS | 40.0% |
| !! | GTTGGTGTTATTGGTGGTAA+TGG | + | chr4.2:92291380-92291399 | MS.gene053189:CDS | 40.0% |
| ATGATCCAGATTGGGGCTAT+TGG | + | chr4.2:92292401-92292420 | MS.gene053189:CDS | 45.0% | |
| ATTGGGAAGAATGGATGGGT+TGG | + | chr4.2:92292419-92292438 | MS.gene053189:CDS | 45.0% | |
| ATTGGGGCTATTGGGAAGAA+TGG | + | chr4.2:92292410-92292429 | MS.gene053189:CDS | 45.0% | |
| GGCTATTGGGAAGAATGGAT+GGG | + | chr4.2:92292415-92292434 | MS.gene053189:CDS | 45.0% | |
| GTGCAAAGGAAGAGATTGTG+GGG | + | chr4.2:92291752-92291771 | MS.gene053189:CDS | 45.0% | |
| GTGTGCAAAGGAAGAGATTG+TGG | + | chr4.2:92291750-92291769 | MS.gene053189:CDS | 45.0% | |
| TACCATCCAGATGTGTGCAA+AGG | + | chr4.2:92291738-92291757 | MS.gene053189:CDS | 45.0% | |
| TCTCTTCCTTTGCACACATC+TGG | - | chr4.2:92291747-92291766 | None:intergenic | 45.0% | |
| TGATCCAGATTGGGGCTATT+GGG | + | chr4.2:92292402-92292421 | MS.gene053189:CDS | 45.0% | |
| TTCCTTTGCACACATCTGGA+TGG | - | chr4.2:92291743-92291762 | None:intergenic | 45.0% | |
| ! | TTGGGAAGAATGGATGGGTT+GGG | + | chr4.2:92292420-92292439 | MS.gene053189:CDS | 45.0% |
| !! | ACATCAGATTCAGAAGCACC+AGG | - | chr4.2:92291530-92291549 | None:intergenic | 45.0% |
| !! | ACTGCTGTTGGTGTTATTGG+TGG | + | chr4.2:92291374-92291393 | MS.gene053189:CDS | 45.0% |
| ATGGCTGCTACTACTGCTGT+TGG | + | chr4.2:92291362-92291381 | MS.gene053189:CDS | 50.0% | |
| GGGCTATTGGGAAGAATGGA+TGG | + | chr4.2:92292414-92292433 | MS.gene053189:CDS | 50.0% | |
| TCTTCCCAATAGCCCCAATC+TGG | - | chr4.2:92292409-92292428 | None:intergenic | 50.0% | |
| !! | GAAGAATGGATGGGTTGGGA+AGG | + | chr4.2:92292424-92292443 | MS.gene053189:CDS | 50.0% |
| !! | TGGATGGGTTGGGAAGGAGC+AGG | + | chr4.2:92292430-92292449 | MS.gene053189:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 92291362 | 92292492 | 92291362 | ID=MS.gene053189 |
| chr4.2 | mRNA | 92291362 | 92292492 | 92291362 | ID=MS.gene053189.t1;Parent=MS.gene053189 |
| chr4.2 | exon | 92291362 | 92291547 | 92291362 | ID=MS.gene053189.t1.exon1;Parent=MS.gene053189.t1 |
| chr4.2 | CDS | 92291362 | 92291547 | 92291362 | ID=cds.MS.gene053189.t1;Parent=MS.gene053189.t1 |
| chr4.2 | exon | 92291738 | 92291806 | 92291738 | ID=MS.gene053189.t1.exon2;Parent=MS.gene053189.t1 |
| chr4.2 | CDS | 92291738 | 92291806 | 92291738 | ID=cds.MS.gene053189.t1;Parent=MS.gene053189.t1 |
| chr4.2 | exon | 92292310 | 92292492 | 92292310 | ID=MS.gene053189.t1.exon3;Parent=MS.gene053189.t1 |
| chr4.2 | CDS | 92292310 | 92292492 | 92292310 | ID=cds.MS.gene053189.t1;Parent=MS.gene053189.t1 |
| Gene Sequence |
| Protein sequence |
