AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66974

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66974.t1 MTR_6g032970 95.298 638 15 3 1 636 18 642 0.0 1231
MS.gene66974.t1 MTR_7g085500 60.185 648 213 15 1 616 15 649 0.0 703
MS.gene66974.t1 MTR_8g088390 45.950 605 267 22 1 570 15 594 1.51e-149 448
MS.gene66974.t1 MTR_8g088390 49.516 517 228 17 71 570 29 529 4.09e-147 439
MS.gene66974.t1 MTR_4g005110 38.129 556 246 19 50 567 70 565 4.40e-96 308
MS.gene66974.t1 MTR_2g032660 51.765 255 108 4 313 563 293 536 1.18e-79 266
MS.gene66974.t1 MTR_2g063460 52.846 246 86 4 331 566 322 547 2.77e-79 263
MS.gene66974.t1 MTR_2g063460 52.846 246 86 4 331 566 320 545 3.12e-79 262
MS.gene66974.t1 MTR_4g123630 50.360 278 109 5 295 563 245 502 2.61e-78 262
MS.gene66974.t1 MTR_2g005840 55.376 186 79 1 382 563 236 421 8.08e-68 229
MS.gene66974.t1 MTR_2g005840 55.376 186 79 1 382 563 267 452 2.50e-67 229
MS.gene66974.t1 MTR_2g029540 26.901 171 111 3 397 563 259 419 1.83e-12 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66974.t1 AT3G13460 43.918 633 284 20 1 580 15 629 7.77e-144 433
MS.gene66974.t1 AT3G13460 43.918 633 284 20 1 580 14 628 8.04e-144 433
MS.gene66974.t1 AT3G13460 43.918 633 284 20 1 580 12 626 1.34e-143 432
MS.gene66974.t1 AT1G55500 41.385 592 284 20 1 559 15 576 4.38e-124 380
MS.gene66974.t1 AT1G55500 41.385 592 284 20 1 559 15 576 5.88e-124 380
MS.gene66974.t1 AT1G55500 41.385 592 284 20 1 559 15 576 9.06e-124 379
MS.gene66974.t1 AT1G55500 43.580 514 242 16 68 559 46 533 8.80e-120 368
MS.gene66974.t1 AT1G55500 40.301 598 269 19 1 559 15 563 3.35e-115 357
MS.gene66974.t1 AT1G55500 41.758 546 249 16 35 559 23 520 9.03e-111 344
MS.gene66974.t1 AT5G61020 52.113 355 152 8 258 606 123 465 6.62e-109 337
MS.gene66974.t1 AT5G61020 52.113 355 152 8 258 606 125 467 8.07e-109 337
MS.gene66974.t1 AT3G03950 51.515 330 125 6 244 565 107 409 2.12e-102 318
MS.gene66974.t1 AT3G03950 51.515 330 125 6 244 565 108 410 2.24e-102 318
MS.gene66974.t1 AT3G03950 51.515 330 125 6 244 565 111 413 2.35e-102 318
MS.gene66974.t1 AT3G13060 48.949 333 142 7 260 567 217 546 1.31e-89 291
MS.gene66974.t1 AT3G13060 48.949 333 142 7 260 567 217 546 1.31e-89 291
MS.gene66974.t1 AT3G13060 48.949 333 142 7 260 567 239 568 1.78e-89 291
MS.gene66974.t1 AT3G13060 48.949 333 142 7 260 567 229 558 1.82e-89 291
MS.gene66974.t1 AT1G27960 50.758 264 104 3 315 578 200 437 2.80e-82 267
MS.gene66974.t1 AT3G17330 59.091 198 75 2 370 563 143 338 9.73e-82 266
MS.gene66974.t1 AT1G27960 50.758 264 104 3 315 578 274 511 1.05e-81 268
MS.gene66974.t1 AT3G17330 59.091 198 75 2 370 563 245 440 7.16e-81 267
MS.gene66974.t1 AT3G17330 59.091 198 75 2 370 563 245 440 7.16e-81 267
MS.gene66974.t1 AT1G48110 61.413 184 67 1 384 563 308 491 3.57e-80 266
MS.gene66974.t1 AT1G48110 61.413 184 67 1 384 563 308 491 3.57e-80 266
MS.gene66974.t1 AT1G79270 63.492 189 65 1 379 563 288 476 4.63e-80 263
MS.gene66974.t1 AT1G79270 63.492 189 65 1 379 563 288 476 4.63e-80 263
MS.gene66974.t1 AT1G79270 63.492 189 65 1 379 563 288 476 4.63e-80 263
MS.gene66974.t1 AT1G79270 63.492 189 65 1 379 563 303 491 6.81e-80 263
MS.gene66974.t1 AT5G58190 54.455 202 81 1 380 570 308 509 3.74e-75 250
MS.gene66974.t1 AT5G58190 54.455 202 81 1 380 570 307 508 3.84e-75 250
MS.gene66974.t1 AT1G09810 55.440 193 82 1 382 570 174 366 5.03e-71 236
MS.gene66974.t1 AT1G09810 55.440 193 82 1 382 570 197 389 1.07e-70 236
MS.gene66974.t1 AT1G09810 55.440 193 82 1 382 570 216 408 1.19e-70 236
MS.gene66974.t1 AT3G13060 46.875 288 125 7 260 522 239 523 2.76e-67 230
MS.gene66974.t1 AT3G13460 36.800 500 249 18 1 451 15 496 1.42e-66 227
MS.gene66974.t1 AT3G13060 38.356 219 107 7 260 453 239 454 4.82e-26 112
MS.gene66974.t1 AT1G27960 43.056 144 56 3 315 458 274 391 2.11e-25 109
MS.gene66974.t1 AT4G11970 29.940 167 100 4 397 553 72 231 6.10e-16 80.5
MS.gene66974.t1 AT4G11970 30.120 166 99 4 398 553 73 231 1.20e-15 79.3
MS.gene66974.t1 AT4G11970 30.120 166 99 4 398 553 73 231 2.47e-15 79.0
MS.gene66974.t1 AT4G11970 30.719 153 94 3 398 545 73 218 2.94e-14 75.9
MS.gene66974.t1 AT4G11970 30.719 153 94 3 398 545 73 218 2.94e-14 75.9

Find 189 sgRNAs with CRISPR-Local

Find 279 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGTGTCTCAACACATTATT+TGG 0.099152 6.4:-3993865 None:intergenic
ATGAACTAACAGGGTGTTTA+TGG 0.166348 6.4:+3992142 MS.gene66974:intron
CATTAGCATTCACATAATTT+TGG 0.172062 6.4:-3991735 None:intergenic
CCTTCTATTTCATACTATTA+TGG 0.211520 6.4:+3991560 MS.gene66974:CDS
TCAGGATATGCTGGTACTTA+TGG 0.214404 6.4:+3992520 MS.gene66974:CDS
TCAATCCTCACCTTCTTTGC+AGG 0.241170 6.4:-3991390 None:intergenic
TCTGGATTGGGACCGTATTC+TGG 0.250558 6.4:+3993083 MS.gene66974:CDS
CCCTTCGTCAGTTATGAATA+AGG 0.260504 6.4:+3992402 MS.gene66974:CDS
CTCTGTATGTGTTTCCATAT+TGG 0.272710 6.4:-3993145 None:intergenic
CCACATGATGATAAGCTCTA+TGG 0.283321 6.4:+3992250 MS.gene66974:CDS
TCTAACTATGGATCTACTTA+TGG 0.286333 6.4:+3993173 MS.gene66974:CDS
TCTAGCTTCTTGTTGCCATT+AGG 0.298283 6.4:-3993551 None:intergenic
CTAGCTTCTTGTTGCCATTA+GGG 0.298390 6.4:-3993550 None:intergenic
CTCTGGAACTGCGATGGTCC+TGG 0.298594 6.4:-3991367 None:intergenic
CTCACCTTCTTTGCAGGCTC+TGG 0.306715 6.4:-3991384 None:intergenic
CAAAATTATGTGAATGCTAA+TGG 0.314548 6.4:+3991736 MS.gene66974:CDS
TGTGAAGTGGCGTATCATTA+AGG 0.316567 6.4:+3993837 MS.gene66974:CDS
CCAGTGTACTGTTGCTTGTC+AGG 0.322359 6.4:-3993422 None:intergenic
AACTTGCATGGTTATTCATC+TGG 0.334371 6.4:+3993065 MS.gene66974:CDS
CCATAATAGTATGAAATAGA+AGG 0.335740 6.4:-3991560 None:intergenic
TACATGTACCCTCAAGGTTA+TGG 0.342289 6.4:+3992178 MS.gene66974:CDS
GAAGCTAGATGCGGCATATC+AGG 0.346860 6.4:+3993565 MS.gene66974:CDS
CCGTATTCTGGATATCCTAA+TGG 0.351387 6.4:+3993095 MS.gene66974:CDS
GTGTCCCTACTGTTTGTAAC+AGG 0.352618 6.4:-3993913 None:intergenic
CATGGTTATTCATCTGGATT+GGG 0.357797 6.4:+3993071 MS.gene66974:CDS
GGGACATGTTCAGCAGTAAC+AGG 0.361006 6.4:-3992382 None:intergenic
CAGGATATGCTGGTACTTAT+GGG 0.362313 6.4:+3992521 MS.gene66974:CDS
TTGGGAACATAGCTAGCATT+TGG 0.365013 6.4:-3991530 None:intergenic
TCCAACAGTTGCCGAATCAT+TGG 0.369473 6.4:-3994318 None:intergenic
TTATCATCATCACTCTTGAT+AGG 0.378740 6.4:-3993383 None:intergenic
TCTCAGCTAAACCAACAAAT+TGG 0.380241 6.4:-3993744 None:intergenic
TGGGAGGGATGGAGATGTGC+TGG 0.383201 6.4:-3992230 None:intergenic
AACACGATGTACAGCCAATA+TGG 0.389273 6.4:+3993131 MS.gene66974:CDS
AAGGTTCCATCCTGAGATTT+CGG 0.391126 6.4:-3994353 None:intergenic
GGATCTATACTTGTTATCAT+AGG 0.391295 6.4:-3993210 None:intergenic
GCATGGTTATTCATCTGGAT+TGG 0.395835 6.4:+3993070 MS.gene66974:CDS
TACTGTTGCTTGTCAGGAAC+TGG 0.401170 6.4:-3993416 None:intergenic
GCATTTGGATTAAATGTCTT+CGG 0.405167 6.4:-3991515 None:intergenic
TTGCTTGGGTACATCCCATT+AGG 0.407428 6.4:-3993110 None:intergenic
CCGAGTATTGCGGAAGTGGC+TGG 0.408284 6.4:-3992672 None:intergenic
TCTACCACTGCAGGCTATGA+TGG 0.409830 6.4:+3991682 MS.gene66974:intron
CACGGCTGGCGTAGAATATC+CGG 0.425172 6.4:-3992608 None:intergenic
AAATCTTTGCGAGGCACGGC+TGG 0.427358 6.4:-3992622 None:intergenic
AATACTAGTGGCCAATTTGT+TGG 0.428966 6.4:+3993733 MS.gene66974:CDS
TACAGAGGTAGGTCTAACTA+TGG 0.433563 6.4:+3993161 MS.gene66974:CDS
CCTGCGTATGCTCCCGTGTC+AGG 0.438298 6.4:+3992502 MS.gene66974:CDS
GCTGATAATAAGAAGGATAC+TGG 0.440767 6.4:-3992285 None:intergenic
TCTGGAACTGCGATGGTCCT+GGG 0.442296 6.4:-3991366 None:intergenic
CGTATTCTGGATATCCTAAT+GGG 0.442464 6.4:+3993096 MS.gene66974:CDS
GGCAAGGCGATTGGAACGAT+TGG 0.444785 6.4:+3991704 MS.gene66974:CDS
TGTGTCTCAACACATTATTT+GGG 0.447474 6.4:-3993864 None:intergenic
GCTTCTGCCAATGGGACATA+TGG 0.454185 6.4:+3992313 MS.gene66974:CDS
TTTAATGCAGAATTTGTCCT+TGG 0.454824 6.4:+3991332 None:intergenic
GGTTATGGTTATGCACCATA+TGG 0.457194 6.4:+3992193 MS.gene66974:CDS
CCTTCGTCAGTTATGAATAA+GGG 0.463151 6.4:+3992403 MS.gene66974:CDS
GAAATAGAAGGGTGTCCATT+GGG 0.463306 6.4:-3991548 None:intergenic
TGCAAAATCATCCAGGATGC+AGG 0.464522 6.4:-3994084 None:intergenic
CCTTGGATTCTGCTGAGCCC+AGG 0.465058 6.4:+3991349 MS.gene66974:CDS
GGCTCGCGAAAAGACAATTC+AGG 0.466070 6.4:+3994115 MS.gene66974:CDS
CAGCCTTCAGCTTCTGCCAA+TGG 0.466914 6.4:+3992304 MS.gene66974:CDS
TGGCTGTACATCGTGTTGCT+TGG 0.469761 6.4:-3993125 None:intergenic
AAAAGAAATGTGGATGGCTT+TGG 0.470712 6.4:+3993263 MS.gene66974:CDS
TCGAGTATTGGCAGCAGGAC+AGG 0.470894 6.4:+3993797 MS.gene66974:CDS
TGATGTATTGCCGAAATCTC+AGG 0.471132 6.4:+3994343 MS.gene66974:CDS
TTGCGGAAGTGGCTGGGAAA+GGG 0.474062 6.4:-3992665 None:intergenic
GGCTGTACATCGTGTTGCTT+GGG 0.474945 6.4:-3993124 None:intergenic
TTCCAGATGCTGCAGGGAAG+GGG 0.485928 6.4:+3994393 MS.gene66974:CDS
TGGCAGCAGGACAGGTGGAC+TGG 0.486046 6.4:+3993805 MS.gene66974:CDS
TCAGTTGTTCCAGATGCTGC+AGG 0.486275 6.4:+3994386 MS.gene66974:CDS
TTCAAGCAAAACCTGCATCC+TGG 0.486649 6.4:+3994073 MS.gene66974:CDS
ACGGTCCTGTCAAAATCAAC+CGG 0.486730 6.4:-3993775 None:intergenic
GCTATGATGGGCAAGGCGAT+TGG 0.487023 6.4:+3991695 MS.gene66974:CDS
TCTACTTATGGATCAAGAAC+AGG 0.487738 6.4:+3993185 MS.gene66974:CDS
AGCCTTCAGCTTCTGCCAAT+GGG 0.488015 6.4:+3992305 MS.gene66974:CDS
CATACCGAGTATTGCGGAAG+TGG 0.490888 6.4:-3992676 None:intergenic
AGTTCCAGAGCCTGCAAAGA+AGG 0.493031 6.4:+3991380 MS.gene66974:CDS
GTCAAATGATTCTTATCAAA+GGG 0.493738 6.4:+3992471 MS.gene66974:CDS
GTGTGGACAAGTACCCCTAA+TGG 0.497541 6.4:+3993536 MS.gene66974:CDS
TTGACAGGACCGTCGAGTAT+TGG 0.499265 6.4:+3993785 MS.gene66974:CDS
CGACTGCTTGACGCTGTCAC+TGG 0.499341 6.4:-3994486 None:intergenic
GTGCAGGTCAAGTTTGAGAA+AGG 0.503701 6.4:+3994020 MS.gene66974:intron
CCATTAGGATATCCAGAATA+CGG 0.504485 6.4:-3993095 None:intergenic
TTCTATGCTCAGGCTGGACA+TGG 0.504827 6.4:+3991476 MS.gene66974:intron
GTTCCAGATGCTGCAGGGAA+GGG 0.505741 6.4:+3994392 MS.gene66974:CDS
CCCTTGAGTAATAATGTGAC+AGG 0.506554 6.4:-3993350 None:intergenic
GGTCAACAAGTCCAATGATT+CGG 0.507129 6.4:+3994307 MS.gene66974:intron
GTGCATAACCATAACCTTGA+GGG 0.508988 6.4:-3992186 None:intergenic
TCCAATGATTCGGCAACTGT+TGG 0.509616 6.4:+3994317 MS.gene66974:CDS
CCGGGGTTGCAGAACTTGCA+TGG 0.509749 6.4:+3993053 MS.gene66974:intron
ACAGTCAGCAGACTTCTCCT+TGG 0.511251 6.4:-3993591 None:intergenic
CCAGCCACTTCCGCAATACT+CGG 0.512463 6.4:+3992672 MS.gene66974:CDS
TGTTCCAGATGCTGCAGGGA+AGG 0.513943 6.4:+3994391 MS.gene66974:CDS
TGTCAAATGATTCTTATCAA+AGG 0.516149 6.4:+3992470 MS.gene66974:CDS
ATTTGTTGGTTTAGCTGAGA+TGG 0.517129 6.4:+3993747 MS.gene66974:CDS
AATGATTCTTATCAAAGGGC+TGG 0.517352 6.4:+3992475 MS.gene66974:CDS
CGAGTATTGCGGAAGTGGCT+GGG 0.519876 6.4:-3992671 None:intergenic
GAAAATAGCAAGGGATCTGA+AGG 0.521253 6.4:-3992559 None:intergenic
ACCTGTCACATTATTACTCA+AGG 0.521502 6.4:+3993349 MS.gene66974:CDS
TAGCTTCTTGTTGCCATTAG+GGG 0.524738 6.4:-3993549 None:intergenic
CACATGATGATAAGCTCTAT+GGG 0.526173 6.4:+3992251 MS.gene66974:CDS
GCGCCACCACTATGACCAAT+GGG 0.531026 6.4:+3992428 MS.gene66974:CDS
CACATTATTACTCAAGGGAC+AGG 0.531212 6.4:+3993355 MS.gene66974:CDS
GGGAGGGATGGAGATGTGCT+GGG 0.532000 6.4:-3992229 None:intergenic
GATGGAGATGTGCTGGGTGA+CGG 0.533067 6.4:-3992223 None:intergenic
AAGTATAGATCCACAAGCTA+TGG 0.534547 6.4:+3993221 MS.gene66974:CDS
CATCATCACTCTTGATAGGA+AGG 0.537515 6.4:-3993379 None:intergenic
ATTGCGGAAGTGGCTGGGAA+AGG 0.537708 6.4:-3992666 None:intergenic
TGAAATAGAAGGGTGTCCAT+TGG 0.537864 6.4:-3991549 None:intergenic
CGTAAAATGCAAAATCATCC+AGG 0.538163 6.4:-3994091 None:intergenic
CTGACAAGCAACAGTACACT+GGG 0.540879 6.4:+3993423 MS.gene66974:CDS
TTTAGCTGAGATGGTGAGTC+CGG 0.541233 6.4:+3993756 MS.gene66974:CDS
AAGGGATCTGAAGGGTATGA+TGG 0.544914 6.4:-3992550 None:intergenic
GACCGTCGAGTATTGGCAGC+AGG 0.549819 6.4:+3993792 MS.gene66974:CDS
AAAACCACGGCCATAGCTTG+TGG 0.550708 6.4:-3993231 None:intergenic
CCTGACAAGCAACAGTACAC+TGG 0.550865 6.4:+3993422 MS.gene66974:CDS
TGCAGGCTCTGGAACTGCGA+TGG 0.552031 6.4:-3991373 None:intergenic
GTTATTTGAAGAATACCCAT+TGG 0.552812 6.4:-3992443 None:intergenic
CTACCACTGCAGGCTATGAT+GGG 0.556798 6.4:+3991683 MS.gene66974:intron
TCCAGATGCTGCAGGGAAGG+GGG 0.562548 6.4:+3994394 MS.gene66974:CDS
TTGTTACAGGTCAATACTAG+TGG 0.563135 6.4:+3993721 MS.gene66974:intron
GCTGAAGGCTGATAATAAGA+AGG 0.564163 6.4:-3992292 None:intergenic
AGAATACCCATTGGTCATAG+TGG 0.567619 6.4:-3992434 None:intergenic
GCTCCCGTGTCAGGATATGC+TGG 0.569566 6.4:+3992511 MS.gene66974:CDS
CATAATAGTATGAAATAGAA+GGG 0.572704 6.4:-3991559 None:intergenic
TCTATTTCATACTATTATGG+AGG 0.576840 6.4:+3991563 MS.gene66974:CDS
TGCTAATGGAGGAATGGCAC+AGG 0.576951 6.4:+3991750 MS.gene66974:CDS
CCTGGGCTCAGCAGAATCCA+AGG 0.578442 6.4:-3991349 None:intergenic
GAGAAGCTTGTAGTAGCGAA+TGG 0.579748 6.4:+3994416 MS.gene66974:CDS
ACAAACCTGTTACAAACAGT+AGG 0.580782 6.4:+3993908 MS.gene66974:CDS
AGTAGCACCATATGTCCCAT+TGG 0.583684 6.4:-3992320 None:intergenic
AGCGCCACCACTATGACCAA+TGG 0.583741 6.4:+3992427 MS.gene66974:CDS
CCATAGAGCTTATCATCATG+TGG 0.585124 6.4:-3992250 None:intergenic
CCCTTATTCATAACTGACGA+AGG 0.585525 6.4:-3992403 None:intergenic
GAAATCTCAGGATGGAACCT+TGG 0.585908 6.4:+3994355 MS.gene66974:CDS
TTATCATCATGTGGGAGGGA+TGG 0.589449 6.4:-3992241 None:intergenic
ATGGAAACACATACAGAGGT+AGG 0.589922 6.4:+3993150 MS.gene66974:CDS
GGAACAACTGTCAAAAGAGA+TGG 0.592068 6.4:+3994151 MS.gene66974:CDS
GGACTTATCAGAAAATAGCA+AGG 0.592605 6.4:-3992569 None:intergenic
AAGGGATGTATTCCTTTGAG+AGG 0.593242 6.4:-3992647 None:intergenic
CAAACCTGTTACAAACAGTA+GGG 0.595379 6.4:+3993909 MS.gene66974:CDS
GGTGACGGATACGATCCATA+TGG 0.596602 6.4:-3992208 None:intergenic
GTCCCATTGGCAGAAGCTGA+AGG 0.601718 6.4:-3992307 None:intergenic
GGTTCTTACATGTACCCTCA+AGG 0.603079 6.4:+3992172 MS.gene66974:CDS
TGTGAATGCTAATGGAGGAA+TGG 0.603286 6.4:+3991744 MS.gene66974:CDS
CAGCTGTTCGAAGAGTCTGC+AGG 0.603437 6.4:-3994458 None:intergenic
CCTTATTCATAACTGACGAA+GGG 0.607312 6.4:-3992402 None:intergenic
GGACATGTTCAGCAGTAACA+GGG 0.609563 6.4:-3992381 None:intergenic
TGGCTTTGGTGAGCTGAACA+AGG 0.609686 6.4:+3993277 MS.gene66974:CDS
CCTGACACGGGAGCATACGC+AGG 0.610725 6.4:-3992502 None:intergenic
GCTAACAAGACCAATGTCCA+TGG 0.611195 6.4:+3992343 MS.gene66974:CDS
TGTCATCAAATCTTATAGCG+AGG 0.611587 6.4:+3993484 MS.gene66974:CDS
GTCCTGCTGCCAATACTCGA+CGG 0.611660 6.4:-3993794 None:intergenic
AGAGTATGAAATATAATGTG+TGG 0.612407 6.4:+3993519 MS.gene66974:CDS
AATGCTAGCTATGTTCCCAA+TGG 0.615673 6.4:+3991533 MS.gene66974:CDS
TGACCATGTCAAAAGAAATG+TGG 0.616421 6.4:+3993253 MS.gene66974:CDS
TCAGAACTATTGGAACCTCT+CGG 0.617068 6.4:-3993302 None:intergenic
GAGCTTATCATCATGTGGGA+GGG 0.621502 6.4:-3992245 None:intergenic
TGCGGCATATCAGGAAGCCA+AGG 0.621638 6.4:+3993574 MS.gene66974:CDS
GCAGGCGGTTCAGTAGAAGT+AGG 0.622553 6.4:-3994440 None:intergenic
CAGGGTGTTTATGGAGACAG+TGG 0.623670 6.4:+3992151 MS.gene66974:intron
AGATCCACAAGCTATGGCCG+TGG 0.631317 6.4:+3993227 MS.gene66974:CDS
AATTATGTGAATGCTAATGG+AGG 0.632378 6.4:+3991739 MS.gene66974:CDS
ACTGCAGGCTATGATGGGCA+AGG 0.633650 6.4:+3991688 MS.gene66974:intron
AGAGCTTATCATCATGTGGG+AGG 0.634564 6.4:-3992246 None:intergenic
GACTTATCAGAAAATAGCAA+GGG 0.638118 6.4:-3992568 None:intergenic
CAGTTGTTCCAGATGCTGCA+GGG 0.638776 6.4:+3994387 MS.gene66974:CDS
AAACAGTAGGGACACTCAAG+AGG 0.639267 6.4:+3993921 MS.gene66974:CDS
ATACCCATTGGTCATAGTGG+TGG 0.639761 6.4:-3992431 None:intergenic
AGTATTGGCAGCAGGACAGG+TGG 0.642857 6.4:+3993800 MS.gene66974:CDS
ACAAGACCAATGTCCATGGA+GGG 0.644910 6.4:+3992347 MS.gene66974:CDS
CCTGTCACATTATTACTCAA+GGG 0.645940 6.4:+3993350 MS.gene66974:CDS
GGTGCATAACCATAACCTTG+AGG 0.647507 6.4:-3992187 None:intergenic
TGGACATGGTCAAGCTAACG+AGG 0.651952 6.4:+3991490 MS.gene66974:CDS
CATAGAGCTTATCATCATGT+GGG 0.652659 6.4:-3992249 None:intergenic
GGCTTTGGTGAGCTGAACAA+GGG 0.653383 6.4:+3993278 MS.gene66974:CDS
CTGTTCGAAGAGTCTGCAGG+CGG 0.658211 6.4:-3994455 None:intergenic
AAAATAGCAAGGGATCTGAA+GGG 0.667653 6.4:-3992558 None:intergenic
AGTGGACATACCGAGTATTG+CGG 0.671258 6.4:-3992682 None:intergenic
AACAACTGATTCATTCACCA+AGG 0.671415 6.4:-3994372 None:intergenic
AGTACCAGCATATCCTGACA+CGG 0.678276 6.4:-3992515 None:intergenic
AACAAGACCAATGTCCATGG+AGG 0.679154 6.4:+3992346 MS.gene66974:CDS
CATGTCAAAAGAAATGTGGA+TGG 0.682176 6.4:+3993257 MS.gene66974:CDS
GACATGTTCAGCAGTAACAG+GGG 0.687021 6.4:-3992380 None:intergenic
CAATATGGAAACACATACAG+AGG 0.690497 6.4:+3993146 MS.gene66974:CDS
GTACCAGCATATCCTGACAC+GGG 0.691496 6.4:-3992514 None:intergenic
GAGCTGAACAAGGGACCGAG+AGG 0.693881 6.4:+3993287 MS.gene66974:CDS
TGGCAACAAGAAGCTAGATG+CGG 0.702216 6.4:+3993556 MS.gene66974:CDS
GTATTGCCGAAATCTCAGGA+TGG 0.724057 6.4:+3994347 MS.gene66974:CDS
TGACAAGCAACAGTACACTG+GGG 0.826136 6.4:+3993424 MS.gene66974:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATTCATTGTATAAAAA+CGG - chr6.4:3992073-3992092 None:intergenic 10.0%
!! AGAAAAAATGATGTATTTAT+GGG + chr6.4:3991655-3991674 MS.gene66974:intron 15.0%
!! TAGAAAAAATGATGTATTTA+TGG + chr6.4:3991654-3991673 MS.gene66974:intron 15.0%
!! AATGAAATGAGTAATTGTAA+AGG - chr6.4:3994183-3994202 None:intergenic 20.0%
!! ACTAGATTACTAAAAAGTTA+AGG - chr6.4:3992027-3992046 None:intergenic 20.0%
!! CATAATAGTATGAAATAGAA+GGG - chr6.4:3991562-3991581 None:intergenic 20.0%
!! CTTCAAAAAGTAATAAATCA+TGG - chr6.4:3991921-3991940 None:intergenic 20.0%
!! TTAGTTCATTAAACAAAACA+TGG - chr6.4:3992131-3992150 None:intergenic 20.0%
!! TTCATATATAAATAGAGAGA+TGG - chr6.4:3992860-3992879 None:intergenic 20.0%
!!! ATTTTAACACATCTTAATTG+AGG + chr6.4:3994204-3994223 MS.gene66974:intron 20.0%
!!! ATTTTCATGATATTTGATTC+TGG + chr6.4:3992880-3992899 MS.gene66974:intron 20.0%
!!! ATTTTTATCATCAGAACTAT+TGG - chr6.4:3993315-3993334 None:intergenic 20.0%
!!! CATGATTTATTACTTTTTGA+AGG + chr6.4:3991919-3991938 MS.gene66974:intron 20.0%
!!! TGAAATTCTCAAAATTTTCA+AGG + chr6.4:3994046-3994065 MS.gene66974:CDS 20.0%
!!! TTTTGTTTAATGAACTAACA+GGG + chr6.4:3992133-3992152 MS.gene66974:intron 20.0%
! ACAAACATTTATGCTTCTAT+TGG + chr6.4:3992996-3993015 MS.gene66974:intron 25.0%
! ACCGAAAATAGCAAAAATAT+GGG - chr6.4:3993617-3993636 None:intergenic 25.0%
! AGAGTATGAAATATAATGTG+TGG + chr6.4:3993519-3993538 MS.gene66974:CDS 25.0%
! CAAAATTATGTGAATGCTAA+TGG + chr6.4:3991736-3991755 MS.gene66974:CDS 25.0%
! CCATAATAGTATGAAATAGA+AGG - chr6.4:3991563-3991582 None:intergenic 25.0%
! CCTTCTATTTCATACTATTA+TGG + chr6.4:3991560-3991579 MS.gene66974:CDS 25.0%
! GATAAAAATGACAAAAGTCT+TGG + chr6.4:3993326-3993345 MS.gene66974:CDS 25.0%
! GTCAAATGATTCTTATCAAA+GGG + chr6.4:3992471-3992490 MS.gene66974:CDS 25.0%
! TCTATTTCATACTATTATGG+AGG + chr6.4:3991563-3991582 MS.gene66974:CDS 25.0%
! TGGTAAAACAGAGAAAATAT+AGG - chr6.4:3993676-3993695 None:intergenic 25.0%
! TGTCAAATGATTCTTATCAA+AGG + chr6.4:3992470-3992489 MS.gene66974:CDS 25.0%
!! CATTAGCATTCACATAATTT+TGG - chr6.4:3991738-3991757 None:intergenic 25.0%
!! TATATTTTCTCTGTTTTACC+AGG + chr6.4:3993675-3993694 MS.gene66974:intron 25.0%
!!! ACTTTAAAATCTGATGATGT+GGG + chr6.4:3991875-3991894 MS.gene66974:intron 25.0%
!!! GTTTTGTTTAATGAACTAAC+AGG + chr6.4:3992132-3992151 MS.gene66974:intron 25.0%
!!! TCCCATATTTTTGCTATTTT+CGG + chr6.4:3993613-3993632 MS.gene66974:CDS 25.0%
AACATGGTTAAAATTGCTCA+AGG - chr6.4:3992115-3992134 None:intergenic 30.0%
AATTATGTGAATGCTAATGG+AGG + chr6.4:3991739-3991758 MS.gene66974:CDS 30.0%
AATTCAGGAGAAAAAAGCTA+AGG + chr6.4:3994130-3994149 MS.gene66974:CDS 30.0%
ATGTGTCTCAACACATTATT+TGG - chr6.4:3993868-3993887 None:intergenic 30.0%
CAAATACCTTGTTCAATTAC+AGG + chr6.4:3994286-3994305 MS.gene66974:intron 30.0%
CACCGAAAATAGCAAAAATA+TGG - chr6.4:3993618-3993637 None:intergenic 30.0%
GACTTATCAGAAAATAGCAA+GGG - chr6.4:3992571-3992590 None:intergenic 30.0%
GCATTTGGATTAAATGTCTT+CGG - chr6.4:3991518-3991537 None:intergenic 30.0%
GTTATTTGAAGAATACCCAT+TGG - chr6.4:3992446-3992465 None:intergenic 30.0%
TATGACTTGAGTGGATAATT+TGG + chr6.4:3991599-3991618 MS.gene66974:intron 30.0%
TCTAACTATGGATCTACTTA+TGG + chr6.4:3993173-3993192 MS.gene66974:CDS 30.0%
TGTGTCTCAACACATTATTT+GGG - chr6.4:3993867-3993886 None:intergenic 30.0%
TTATCATCATCACTCTTGAT+AGG - chr6.4:3993386-3993405 None:intergenic 30.0%
TTCAATTGTGAGCTTAAGAT+AGG - chr6.4:3992823-3992842 None:intergenic 30.0%
TTCATACTATTATGGAGGTA+AGG + chr6.4:3991568-3991587 MS.gene66974:intron 30.0%
! CTGTAATTGAACAAGGTATT+TGG - chr6.4:3994288-3994307 None:intergenic 30.0%
! GTTAAGCATTGTTTGTTGAA+AGG - chr6.4:3993989-3994008 None:intergenic 30.0%
!! GACTTTAAAATCTGATGATG+TGG + chr6.4:3991874-3991893 MS.gene66974:intron 30.0%
!! GGATCTATACTTGTTATCAT+AGG - chr6.4:3993213-3993232 None:intergenic 30.0%
!! TATAATCTGTTTCTATGCTC+AGG + chr6.4:3991466-3991485 MS.gene66974:intron 30.0%
AAAAGAAATGTGGATGGCTT+TGG + chr6.4:3993263-3993282 MS.gene66974:CDS 35.0%
AAAATAGCAAGGGATCTGAA+GGG - chr6.4:3992561-3992580 None:intergenic 35.0%
AAATCATGAGGCAAAAAGTG+AGG - chr6.4:3993701-3993720 None:intergenic 35.0%
AACTTGCATGGTTATTCATC+TGG + chr6.4:3993065-3993084 MS.gene66974:CDS 35.0%
AAGTATAGATCCACAAGCTA+TGG + chr6.4:3993221-3993240 MS.gene66974:CDS 35.0%
AATACTAGTGGCCAATTTGT+TGG + chr6.4:3993733-3993752 MS.gene66974:CDS 35.0%
AATGATTCTTATCAAAGGGC+TGG + chr6.4:3992475-3992494 MS.gene66974:CDS 35.0%
AATGCTTAGATCCAGTCTAA+AGG - chr6.4:3992728-3992747 None:intergenic 35.0%
ACAAACCTGTTACAAACAGT+AGG + chr6.4:3993908-3993927 MS.gene66974:CDS 35.0%
ACCTGTCACATTATTACTCA+AGG + chr6.4:3993349-3993368 MS.gene66974:CDS 35.0%
ACTATTATGGAGGTAAGGTA+AGG + chr6.4:3991573-3991592 MS.gene66974:intron 35.0%
ACTCACCTTAACTATTGTTC+CGG + chr6.4:3993034-3993053 MS.gene66974:intron 35.0%
CAAACCTGTTACAAACAGTA+GGG + chr6.4:3993909-3993928 MS.gene66974:CDS 35.0%
CAATATGGAAACACATACAG+AGG + chr6.4:3993146-3993165 MS.gene66974:CDS 35.0%
CAATCAAGCTGATGTTGTAA+AGG - chr6.4:3992769-3992788 None:intergenic 35.0%
CACATGATGATAAGCTCTAT+GGG + chr6.4:3992251-3992270 MS.gene66974:CDS 35.0%
CAGTCTAAAGGTTAGAATCA+AGG - chr6.4:3992716-3992735 None:intergenic 35.0%
CATAGAGCTTATCATCATGT+GGG - chr6.4:3992252-3992271 None:intergenic 35.0%
CATGGTTATTCATCTGGATT+GGG + chr6.4:3993071-3993090 MS.gene66974:CDS 35.0%
CATGTCAAAAGAAATGTGGA+TGG + chr6.4:3993257-3993276 MS.gene66974:CDS 35.0%
CCATTAGGATATCCAGAATA+CGG - chr6.4:3993098-3993117 None:intergenic 35.0%
CCTGTCACATTATTACTCAA+GGG + chr6.4:3993350-3993369 MS.gene66974:CDS 35.0%
CCTTATTCATAACTGACGAA+GGG - chr6.4:3992405-3992424 None:intergenic 35.0%
CCTTCGTCAGTTATGAATAA+GGG + chr6.4:3992403-3992422 MS.gene66974:CDS 35.0%
CGTAAAATGCAAAATCATCC+AGG - chr6.4:3994094-3994113 None:intergenic 35.0%
CGTATTCTGGATATCCTAAT+GGG + chr6.4:3993096-3993115 MS.gene66974:CDS 35.0%
CTCTGTATGTGTTTCCATAT+TGG - chr6.4:3993148-3993167 None:intergenic 35.0%
CTTGATTCTAACCTTTAGAC+TGG + chr6.4:3992714-3992733 MS.gene66974:intron 35.0%
GACCTGTAACAAAAATCATG+AGG - chr6.4:3993713-3993732 None:intergenic 35.0%
GCTGATAATAAGAAGGATAC+TGG - chr6.4:3992288-3992307 None:intergenic 35.0%
GGACTTATCAGAAAATAGCA+AGG - chr6.4:3992572-3992591 None:intergenic 35.0%
TCTACTTATGGATCAAGAAC+AGG + chr6.4:3993185-3993204 MS.gene66974:CDS 35.0%
TCTCAGCTAAACCAACAAAT+TGG - chr6.4:3993747-3993766 None:intergenic 35.0%
TGACCATGTCAAAAGAAATG+TGG + chr6.4:3993253-3993272 MS.gene66974:CDS 35.0%
TGTCATCAAATCTTATAGCG+AGG + chr6.4:3993484-3993503 MS.gene66974:CDS 35.0%
TGTTGACCTGTAATTGAACA+AGG - chr6.4:3994295-3994314 None:intergenic 35.0%
TTAACTATATGCTCTTGTGC+AGG + chr6.4:3994004-3994023 MS.gene66974:intron 35.0%
TTGTTACAGGTCAATACTAG+TGG + chr6.4:3993721-3993740 MS.gene66974:intron 35.0%
! AACAACTGATTCATTCACCA+AGG - chr6.4:3994375-3994394 None:intergenic 35.0%
! ATGAACTAACAGGGTGTTTA+TGG + chr6.4:3992142-3992161 MS.gene66974:intron 35.0%
! ATTGTTTGTTGAAAGGAGTG+TGG - chr6.4:3993982-3994001 None:intergenic 35.0%
! CATCCACATTTCTTTTGACA+TGG - chr6.4:3993259-3993278 None:intergenic 35.0%
! GCAAAGATTTTTCCTCTCAA+AGG + chr6.4:3992635-3992654 MS.gene66974:CDS 35.0%
! GGATGATTTTGCATTTTACG+AGG + chr6.4:3994094-3994113 MS.gene66974:CDS 35.0%
! TCTCGATCCAGTATTTTGTT+GGG + chr6.4:3993646-3993665 MS.gene66974:intron 35.0%
! TGCCTCATGATTTTTGTTAC+AGG + chr6.4:3993708-3993727 MS.gene66974:intron 35.0%
! TTGTTTGTTGAAAGGAGTGT+GGG - chr6.4:3993981-3994000 None:intergenic 35.0%
! TTTTCTGATAAGTCCAAGCA+TGG + chr6.4:3992577-3992596 MS.gene66974:CDS 35.0%
! TTTTGACATGGTCAAAACCA+CGG - chr6.4:3993247-3993266 None:intergenic 35.0%
!! ATTTGTTGGTTTAGCTGAGA+TGG + chr6.4:3993747-3993766 MS.gene66974:CDS 35.0%
AACACGATGTACAGCCAATA+TGG + chr6.4:3993131-3993150 MS.gene66974:CDS 40.0%
AAGGGATGTATTCCTTTGAG+AGG - chr6.4:3992650-3992669 None:intergenic 40.0%
AAGGTTCCATCCTGAGATTT+CGG - chr6.4:3994356-3994375 None:intergenic 40.0%
AATAGCACATTGTTCCCAAC+AGG - chr6.4:3991826-3991845 None:intergenic 40.0%
AATGCTAGCTATGTTCCCAA+TGG + chr6.4:3991533-3991552 MS.gene66974:CDS 40.0%
AGAATACCCATTGGTCATAG+TGG - chr6.4:3992437-3992456 None:intergenic 40.0%
AGGAATGCCCAACAAAATAC+TGG - chr6.4:3993656-3993675 None:intergenic 40.0%
AGGTTAGAATCAAGGAGTGA+CGG - chr6.4:3992708-3992727 None:intergenic 40.0%
ATGGAAACACATACAGAGGT+AGG + chr6.4:3993150-3993169 MS.gene66974:CDS 40.0%
CACATTATTACTCAAGGGAC+AGG + chr6.4:3993355-3993374 MS.gene66974:CDS 40.0%
CAGGATATGCTGGTACTTAT+GGG + chr6.4:3992521-3992540 MS.gene66974:CDS 40.0%
CATCATCACTCTTGATAGGA+AGG - chr6.4:3993382-3993401 None:intergenic 40.0%
CCACATGATGATAAGCTCTA+TGG + chr6.4:3992250-3992269 MS.gene66974:CDS 40.0%
CCATAGAGCTTATCATCATG+TGG - chr6.4:3992253-3992272 None:intergenic 40.0%
CCCTTATTCATAACTGACGA+AGG - chr6.4:3992406-3992425 None:intergenic 40.0%
CCCTTCGTCAGTTATGAATA+AGG + chr6.4:3992402-3992421 MS.gene66974:CDS 40.0%
CCCTTGAGTAATAATGTGAC+AGG - chr6.4:3993353-3993372 None:intergenic 40.0%
CCGTATTCTGGATATCCTAA+TGG + chr6.4:3993095-3993114 MS.gene66974:CDS 40.0%
CTAGCTTCTTGTTGCCATTA+GGG - chr6.4:3993553-3993572 None:intergenic 40.0%
CTCACCTTAACTATTGTTCC+GGG + chr6.4:3993035-3993054 MS.gene66974:intron 40.0%
GAAAATAGCAAGGGATCTGA+AGG - chr6.4:3992562-3992581 None:intergenic 40.0%
GACTTGAGTGGATAATTTGG+AGG + chr6.4:3991602-3991621 MS.gene66974:intron 40.0%
GCATGGTTATTCATCTGGAT+TGG + chr6.4:3993070-3993089 MS.gene66974:CDS 40.0%
GCTGAAGGCTGATAATAAGA+AGG - chr6.4:3992295-3992314 None:intergenic 40.0%
GGAACAACTGTCAAAAGAGA+TGG + chr6.4:3994151-3994170 MS.gene66974:CDS 40.0%
GGTCAACAAGTCCAATGATT+CGG + chr6.4:3994307-3994326 MS.gene66974:intron 40.0%
GGTTATGGTTATGCACCATA+TGG + chr6.4:3992193-3992212 MS.gene66974:CDS 40.0%
GTGCATAACCATAACCTTGA+GGG - chr6.4:3992189-3992208 None:intergenic 40.0%
TACAGAGGTAGGTCTAACTA+TGG + chr6.4:3993161-3993180 MS.gene66974:CDS 40.0%
TACATGTACCCTCAAGGTTA+TGG + chr6.4:3992178-3992197 MS.gene66974:CDS 40.0%
TAGCTTCTTGTTGCCATTAG+GGG - chr6.4:3993552-3993571 None:intergenic 40.0%
TCACCTTAACTATTGTTCCG+GGG + chr6.4:3993036-3993055 MS.gene66974:intron 40.0%
TCAGAACTATTGGAACCTCT+CGG - chr6.4:3993305-3993324 None:intergenic 40.0%
TCAGGATATGCTGGTACTTA+TGG + chr6.4:3992520-3992539 MS.gene66974:CDS 40.0%
TCCAAGCATGGATCAAAAAC+CGG + chr6.4:3992589-3992608 MS.gene66974:CDS 40.0%
TCTGGCTATGACTATTGTTG+TGG + chr6.4:3992898-3992917 MS.gene66974:intron 40.0%
TGAGAGGAAAAATCTTTGCG+AGG - chr6.4:3992634-3992653 None:intergenic 40.0%
TGATGTATTGCCGAAATCTC+AGG + chr6.4:3994343-3994362 MS.gene66974:CDS 40.0%
TGTGAAGTGGCGTATCATTA+AGG + chr6.4:3993837-3993856 MS.gene66974:CDS 40.0%
TGTGAATGCTAATGGAGGAA+TGG + chr6.4:3991744-3991763 MS.gene66974:CDS 40.0%
TGTGTGCATATAAGCCTGTT+GGG + chr6.4:3991809-3991828 MS.gene66974:intron 40.0%
TGTTGGGAACAATGTGCTAT+TGG + chr6.4:3991825-3991844 MS.gene66974:intron 40.0%
TTACCTAATTGACGTAGAGC+AGG + chr6.4:3992952-3992971 MS.gene66974:intron 40.0%
TTGGGAACATAGCTAGCATT+TGG - chr6.4:3991533-3991552 None:intergenic 40.0%
TTGTGTGCATATAAGCCTGT+TGG + chr6.4:3991808-3991827 MS.gene66974:intron 40.0%
! AACAATGTGCTATTGGGTGT+TGG + chr6.4:3991832-3991851 MS.gene66974:intron 40.0%
! ACAATGTGCTATTGGGTGTT+GGG + chr6.4:3991833-3991852 MS.gene66974:intron 40.0%
! GTAAGGTGTTATGACTTGAG+TGG + chr6.4:3991590-3991609 MS.gene66974:intron 40.0%
! GTCTCGATCCAGTATTTTGT+TGG + chr6.4:3993645-3993664 MS.gene66974:intron 40.0%
! GTTGGGAACAATGTGCTATT+GGG + chr6.4:3991826-3991845 MS.gene66974:intron 40.0%
! TCTAGCTTCTTGTTGCCATT+AGG - chr6.4:3993554-3993573 None:intergenic 40.0%
! TGTTTGTTGAAAGGAGTGTG+GGG - chr6.4:3993980-3993999 None:intergenic 40.0%
!! GAAATAGAAGGGTGTCCATT+GGG - chr6.4:3991551-3991570 None:intergenic 40.0%
!! TGAAATAGAAGGGTGTCCAT+TGG - chr6.4:3991552-3991571 None:intergenic 40.0%
AAACAGTAGGGACACTCAAG+AGG + chr6.4:3993921-3993940 MS.gene66974:CDS 45.0%
AACAAGACCAATGTCCATGG+AGG + chr6.4:3992346-3992365 MS.gene66974:CDS 45.0%
ACAAGACCAATGTCCATGGA+GGG + chr6.4:3992347-3992366 MS.gene66974:CDS 45.0%
ACGGTCCTGTCAAAATCAAC+CGG - chr6.4:3993778-3993797 None:intergenic 45.0%
AGAGCTTATCATCATGTGGG+AGG - chr6.4:3992249-3992268 None:intergenic 45.0%
AGTACCAGCATATCCTGACA+CGG - chr6.4:3992518-3992537 None:intergenic 45.0%
AGTAGCACCATATGTCCCAT+TGG - chr6.4:3992323-3992342 None:intergenic 45.0%
AGTGTGGGGTTAATGGGAAT+CGG - chr6.4:3993966-3993985 None:intergenic 45.0%
ATACCCATTGGTCATAGTGG+TGG - chr6.4:3992434-3992453 None:intergenic 45.0%
CAACCCCGGAACAATAGTTA+AGG - chr6.4:3993042-3993061 None:intergenic 45.0%
CTGACAAGCAACAGTACACT+GGG + chr6.4:3993423-3993442 MS.gene66974:CDS 45.0%
CTTCCTGCTCTACGTCAATT+AGG - chr6.4:3992958-3992977 None:intergenic 45.0%
GAAATCTCAGGATGGAACCT+TGG + chr6.4:3994355-3994374 MS.gene66974:CDS 45.0%
GACATGTTCAGCAGTAACAG+GGG - chr6.4:3992383-3992402 None:intergenic 45.0%
GAGAAGCTTGTAGTAGCGAA+TGG + chr6.4:3994416-3994435 MS.gene66974:CDS 45.0%
GAGCTTATCATCATGTGGGA+GGG - chr6.4:3992248-3992267 None:intergenic 45.0%
GCTAACAAGACCAATGTCCA+TGG + chr6.4:3992343-3992362 MS.gene66974:CDS 45.0%
GGAAAAATCTTTGCGAGGCA+CGG - chr6.4:3992629-3992648 None:intergenic 45.0%
GGACATGTTCAGCAGTAACA+GGG - chr6.4:3992384-3992403 None:intergenic 45.0%
GGTGCATAACCATAACCTTG+AGG - chr6.4:3992190-3992209 None:intergenic 45.0%
GGTTCTTACATGTACCCTCA+AGG + chr6.4:3992172-3992191 MS.gene66974:CDS 45.0%
GTATTGCCGAAATCTCAGGA+TGG + chr6.4:3994347-3994366 MS.gene66974:CDS 45.0%
GTGCAGGTCAAGTTTGAGAA+AGG + chr6.4:3994020-3994039 MS.gene66974:intron 45.0%
GTGTCCCTACTGTTTGTAAC+AGG - chr6.4:3993916-3993935 None:intergenic 45.0%
TCAATCCTCACCTTCTTTGC+AGG - chr6.4:3991393-3991412 None:intergenic 45.0%
TCCAACAGTTGCCGAATCAT+TGG - chr6.4:3994321-3994340 None:intergenic 45.0%
TGACAAGCAACAGTACACTG+GGG + chr6.4:3993424-3993443 MS.gene66974:CDS 45.0%
TGCAAAATCATCCAGGATGC+AGG - chr6.4:3994087-3994106 None:intergenic 45.0%
TGGCAACAAGAAGCTAGATG+CGG + chr6.4:3993556-3993575 MS.gene66974:CDS 45.0%
TTATCATCATGTGGGAGGGA+TGG - chr6.4:3992244-3992263 None:intergenic 45.0%
TTCAAGCAAAACCTGCATCC+TGG + chr6.4:3994073-3994092 MS.gene66974:CDS 45.0%
TTGCTTGGGTACATCCCATT+AGG - chr6.4:3993113-3993132 None:intergenic 45.0%
TTTAGCTGAGATGGTGAGTC+CGG + chr6.4:3993756-3993775 MS.gene66974:CDS 45.0%
! AAGGGATCTGAAGGGTATGA+TGG - chr6.4:3992553-3992572 None:intergenic 45.0%
! AGTGGACATACCGAGTATTG+CGG - chr6.4:3992685-3992704 None:intergenic 45.0%
! AGTTTTCCCTCCATGGACAT+TGG - chr6.4:3992356-3992375 None:intergenic 45.0%
! GAAAGGAGTGTGGGGTTAAT+GGG - chr6.4:3993972-3993991 None:intergenic 45.0%
! TACTGTTGCTTGTCAGGAAC+TGG - chr6.4:3993419-3993438 None:intergenic 45.0%
! TCCAATGATTCGGCAACTGT+TGG + chr6.4:3994317-3994336 MS.gene66974:CDS 45.0%
! TCCGGTTTTTGATCCATGCT+TGG - chr6.4:3992593-3992612 None:intergenic 45.0%
! TGAAAGGAGTGTGGGGTTAA+TGG - chr6.4:3993973-3993992 None:intergenic 45.0%
!! ATCTGTTTCTATGCTCAGGC+TGG + chr6.4:3991470-3991489 MS.gene66974:intron 45.0%
!! CACTCAAGAGGTATTCACTC+AGG + chr6.4:3993933-3993952 MS.gene66974:intron 45.0%
!! TGAGTCCGGTTGATTTTGAC+AGG + chr6.4:3993770-3993789 MS.gene66974:CDS 45.0%
!!! CTGGTTGCTTTTCTGTGAAG+TGG + chr6.4:3993824-3993843 MS.gene66974:CDS 45.0%
AAAACCACGGCCATAGCTTG+TGG - chr6.4:3993234-3993253 None:intergenic 50.0%
ACAGTCAGCAGACTTCTCCT+TGG - chr6.4:3993594-3993613 None:intergenic 50.0%
AGCCTTCAGCTTCTGCCAAT+GGG + chr6.4:3992305-3992324 MS.gene66974:CDS 50.0%
AGTTCCAGAGCCTGCAAAGA+AGG + chr6.4:3991380-3991399 MS.gene66974:CDS 50.0%
ATGAGGCAAAAAGTGAGGCC+TGG - chr6.4:3993696-3993715 None:intergenic 50.0%
ATGGGAATGTCTACCACTGC+AGG + chr6.4:3991673-3991692 MS.gene66974:intron 50.0%
CAGTTGTTCCAGATGCTGCA+GGG + chr6.4:3994387-3994406 MS.gene66974:CDS 50.0%
CCTGACAAGCAACAGTACAC+TGG + chr6.4:3993422-3993441 MS.gene66974:CDS 50.0%
CTACCACTGCAGGCTATGAT+GGG + chr6.4:3991683-3991702 MS.gene66974:intron 50.0%
GAAGCTAGATGCGGCATATC+AGG + chr6.4:3993565-3993584 MS.gene66974:CDS 50.0%
GCTTCTGCCAATGGGACATA+TGG + chr6.4:3992313-3992332 MS.gene66974:CDS 50.0%
GGCTCGCGAAAAGACAATTC+AGG + chr6.4:3994115-3994134 MS.gene66974:CDS 50.0%
GGGACATGTTCAGCAGTAAC+AGG - chr6.4:3992385-3992404 None:intergenic 50.0%
GTACCAGCATATCCTGACAC+GGG - chr6.4:3992517-3992536 None:intergenic 50.0%
GTGTGGACAAGTACCCCTAA+TGG + chr6.4:3993536-3993555 MS.gene66974:CDS 50.0%
TCAGTTGTTCCAGATGCTGC+AGG + chr6.4:3994386-3994405 MS.gene66974:CDS 50.0%
TCTACCACTGCAGGCTATGA+TGG + chr6.4:3991682-3991701 MS.gene66974:intron 50.0%
TGCTAATGGAGGAATGGCAC+AGG + chr6.4:3991750-3991769 MS.gene66974:CDS 50.0%
TGGACATGGTCAAGCTAACG+AGG + chr6.4:3991490-3991509 MS.gene66974:CDS 50.0%
TTCTATGCTCAGGCTGGACA+TGG + chr6.4:3991476-3991495 MS.gene66974:intron 50.0%
TTGACAGGACCGTCGAGTAT+TGG + chr6.4:3993785-3993804 MS.gene66974:CDS 50.0%
! AGAATCAAGGAGTGACGGAG+TGG - chr6.4:3992703-3992722 None:intergenic 50.0%
! CAGGGTGTTTATGGAGACAG+TGG + chr6.4:3992151-3992170 MS.gene66974:intron 50.0%
! CATACCGAGTATTGCGGAAG+TGG - chr6.4:3992679-3992698 None:intergenic 50.0%
! CCAGTGTACTGTTGCTTGTC+AGG - chr6.4:3993425-3993444 None:intergenic 50.0%
! GGCTTTGGTGAGCTGAACAA+GGG + chr6.4:3993278-3993297 MS.gene66974:CDS 50.0%
! GGGTTGAAGTTTTCCCTCCA+TGG - chr6.4:3992363-3992382 None:intergenic 50.0%
! TCTGGATTGGGACCGTATTC+TGG + chr6.4:3993083-3993102 MS.gene66974:CDS 50.0%
! TGGCTGTACATCGTGTTGCT+TGG - chr6.4:3993128-3993147 None:intergenic 50.0%
!! GGCTGTACATCGTGTTGCTT+GGG - chr6.4:3993127-3993146 None:intergenic 50.0%
!! GGTGACGGATACGATCCATA+TGG - chr6.4:3992211-3992230 None:intergenic 50.0%
!! TGGCTTTGGTGAGCTGAACA+AGG + chr6.4:3993277-3993296 MS.gene66974:CDS 50.0%
AAATCTTTGCGAGGCACGGC+TGG - chr6.4:3992625-3992644 None:intergenic 55.0%
AGATCCACAAGCTATGGCCG+TGG + chr6.4:3993227-3993246 MS.gene66974:CDS 55.0%
AGCGCCACCACTATGACCAA+TGG + chr6.4:3992427-3992446 MS.gene66974:CDS 55.0%
ATTGCGGAAGTGGCTGGGAA+AGG - chr6.4:3992669-3992688 None:intergenic 55.0%
CACGGCTGGCGTAGAATATC+CGG - chr6.4:3992611-3992630 None:intergenic 55.0%
CAGAGCCTGCAAAGAAGGTG+AGG + chr6.4:3991385-3991404 MS.gene66974:intron 55.0%
CAGCCTTCAGCTTCTGCCAA+TGG + chr6.4:3992304-3992323 MS.gene66974:CDS 55.0%
CAGCTGTTCGAAGAGTCTGC+AGG - chr6.4:3994461-3994480 None:intergenic 55.0%
CCAGCCACTTCCGCAATACT+CGG + chr6.4:3992672-3992691 MS.gene66974:CDS 55.0%
CCATGCAAGTTCTGCAACCC+CGG - chr6.4:3993056-3993075 None:intergenic 55.0%
CTCACCTTCTTTGCAGGCTC+TGG - chr6.4:3991387-3991406 None:intergenic 55.0%
CTGTTCGAAGAGTCTGCAGG+CGG - chr6.4:3994458-3994477 None:intergenic 55.0%
GATGGAGATGTGCTGGGTGA+CGG - chr6.4:3992226-3992245 None:intergenic 55.0%
GCAGGCGGTTCAGTAGAAGT+AGG - chr6.4:3994443-3994462 None:intergenic 55.0%
GCGCCACCACTATGACCAAT+GGG + chr6.4:3992428-3992447 MS.gene66974:CDS 55.0%
GGCAAGGCGATTGGAACGAT+TGG + chr6.4:3991704-3991723 MS.gene66974:CDS 55.0%
GTCCCATTGGCAGAAGCTGA+AGG - chr6.4:3992310-3992329 None:intergenic 55.0%
GTCCTGCTGCCAATACTCGA+CGG - chr6.4:3993797-3993816 None:intergenic 55.0%
GTTCCAGATGCTGCAGGGAA+GGG + chr6.4:3994392-3994411 MS.gene66974:CDS 55.0%
TGCGGCATATCAGGAAGCCA+AGG + chr6.4:3993574-3993593 MS.gene66974:CDS 55.0%
TGTTCCAGATGCTGCAGGGA+AGG + chr6.4:3994391-3994410 MS.gene66974:CDS 55.0%
TTCCAGATGCTGCAGGGAAG+GGG + chr6.4:3994393-3994412 MS.gene66974:CDS 55.0%
TTGCCCATCATAGCCTGCAG+TGG - chr6.4:3991689-3991708 None:intergenic 55.0%
TTGCGGAAGTGGCTGGGAAA+GGG - chr6.4:3992668-3992687 None:intergenic 55.0%
! CGAGTATTGCGGAAGTGGCT+GGG - chr6.4:3992674-3992693 None:intergenic 55.0%
! TCTGGAACTGCGATGGTCCT+GGG - chr6.4:3991369-3991388 None:intergenic 55.0%
!! ACTGCAGGCTATGATGGGCA+AGG + chr6.4:3991688-3991707 MS.gene66974:intron 55.0%
!! AGTATTGGCAGCAGGACAGG+TGG + chr6.4:3993800-3993819 MS.gene66974:CDS 55.0%
!! GCTATGATGGGCAAGGCGAT+TGG + chr6.4:3991695-3991714 MS.gene66974:CDS 55.0%
!! TCGAGTATTGGCAGCAGGAC+AGG + chr6.4:3993797-3993816 MS.gene66974:CDS 55.0%
CCGGGGTTGCAGAACTTGCA+TGG + chr6.4:3993053-3993072 MS.gene66974:intron 60.0%
CCTGGGCTCAGCAGAATCCA+AGG - chr6.4:3991352-3991371 None:intergenic 60.0%
CCTTGGATTCTGCTGAGCCC+AGG + chr6.4:3991349-3991368 MS.gene66974:CDS 60.0%
CGACTGCTTGACGCTGTCAC+TGG - chr6.4:3994489-3994508 None:intergenic 60.0%
GAGCTGAACAAGGGACCGAG+AGG + chr6.4:3993287-3993306 MS.gene66974:CDS 60.0%
GCTCCCGTGTCAGGATATGC+TGG + chr6.4:3992511-3992530 MS.gene66974:CDS 60.0%
GGGAGGGATGGAGATGTGCT+GGG - chr6.4:3992232-3992251 None:intergenic 60.0%
TCCAGATGCTGCAGGGAAGG+GGG + chr6.4:3994394-3994413 MS.gene66974:CDS 60.0%
TGGGAGGGATGGAGATGTGC+TGG - chr6.4:3992233-3992252 None:intergenic 60.0%
! CCGAGTATTGCGGAAGTGGC+TGG - chr6.4:3992675-3992694 None:intergenic 60.0%
!! CTCTGGAACTGCGATGGTCC+TGG - chr6.4:3991370-3991389 None:intergenic 60.0%
!! GACCGTCGAGTATTGGCAGC+AGG + chr6.4:3993792-3993811 MS.gene66974:CDS 60.0%
!! TGCAGGCTCTGGAACTGCGA+TGG - chr6.4:3991376-3991395 None:intergenic 60.0%
CCTGCGTATGCTCCCGTGTC+AGG + chr6.4:3992502-3992521 MS.gene66974:CDS 65.0%
TCCCCCTTCCCTGCAGCATC+TGG - chr6.4:3994398-3994417 None:intergenic 65.0%
TGGCAGCAGGACAGGTGGAC+TGG + chr6.4:3993805-3993824 MS.gene66974:CDS 65.0%
! CCTGACACGGGAGCATACGC+AGG - chr6.4:3992505-3992524 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 3991336 3994517 3991336 ID=MS.gene66974
chr6.4 mRNA 3991336 3994517 3991336 ID=MS.gene66974.t1;Parent=MS.gene66974
chr6.4 exon 3991336 3991401 3991336 ID=MS.gene66974.t1.exon1;Parent=MS.gene66974.t1
chr6.4 CDS 3991336 3991401 3991336 ID=cds.MS.gene66974.t1;Parent=MS.gene66974.t1
chr6.4 exon 3991488 3991584 3991488 ID=MS.gene66974.t1.exon2;Parent=MS.gene66974.t1
chr6.4 CDS 3991488 3991584 3991488 ID=cds.MS.gene66974.t1;Parent=MS.gene66974.t1
chr6.4 exon 3991695 3991771 3991695 ID=MS.gene66974.t1.exon3;Parent=MS.gene66974.t1
chr6.4 CDS 3991695 3991771 3991695 ID=cds.MS.gene66974.t1;Parent=MS.gene66974.t1
chr6.4 exon 3992154 3992693 3992154 ID=MS.gene66974.t1.exon4;Parent=MS.gene66974.t1
chr6.4 CDS 3992154 3992693 3992154 ID=cds.MS.gene66974.t1;Parent=MS.gene66974.t1
chr6.4 exon 3993065 3993634 3993065 ID=MS.gene66974.t1.exon5;Parent=MS.gene66974.t1
chr6.4 CDS 3993065 3993634 3993065 ID=cds.MS.gene66974.t1;Parent=MS.gene66974.t1
chr6.4 exon 3993730 3993942 3993730 ID=MS.gene66974.t1.exon6;Parent=MS.gene66974.t1
chr6.4 CDS 3993730 3993942 3993730 ID=cds.MS.gene66974.t1;Parent=MS.gene66974.t1
chr6.4 exon 3994026 3994172 3994026 ID=MS.gene66974.t1.exon7;Parent=MS.gene66974.t1
chr6.4 CDS 3994026 3994172 3994026 ID=cds.MS.gene66974.t1;Parent=MS.gene66974.t1
chr6.4 exon 3994308 3994517 3994308 ID=MS.gene66974.t1.exon8;Parent=MS.gene66974.t1
chr6.4 CDS 3994308 3994517 3994308 ID=cds.MS.gene66974.t1;Parent=MS.gene66974.t1
Gene Sequence

>MS.gene66974

ATGCAGAATTTGTCCTTGGATTCTGCTGAGCCCAGGACCATCGCAGTTCCAGAGCCTGCAAAGAAGGTGAGGATTGAGAAGTGCATTATAGCATTGATGTATATGAAGTTGTCGTCTTTTAATTGACTCTTATAATCTGTTTCTATGCTCAGGCTGGACATGGTCAAGCTAACGAGGTGCCGAAGACATTTAATCCAAATGCTAGCTATGTTCCCAATGGACACCCTTCTATTTCATACTATTATGGAGGTAAGGTAAGGTGTTATGACTTGAGTGGATAATTTGGAGGTTGTTTATTGAAGATATAAGTATTTCTTCTAGAAAAAATGATGTATTTATGGGAATGTCTACCACTGCAGGCTATGATGGGCAAGGCGATTGGAACGATTGGAATGTTTACCAAAATTATGTGAATGCTAATGGAGGAATGGCACAGGTATGTACTTATTCTAGTTACTAGACTTCATGATAATTGTGTGCATATAAGCCTGTTGGGAACAATGTGCTATTGGGTGTTGGGTAATTTCTGTTATATATTGACTTTAAAATCTGATGATGTGGGTTGTTGCTTACTACTAAAAACCATGATTTATTACTTTTTGAAGGTTCAATTTTGTCTCTTTCATAAATGTGAACATTTAAATTGCATTGCATTATTTGTGATTTCTTTTTCTCGATGATCACTAATCCTTAACTTTTTAGTAATCTAGTTTCAGTTTGATCATTTTTGCTTGCCGTTTTTATACAATGAATTATACTTGACAGAATTGTTGAATCCTTGAGCAATTTTAACCATGTTTTGTTTAATGAACTAACAGGGTGTTTATGGAGACAGTGGTTCTTACATGTACCCTCAAGGTTATGGTTATGCACCATATGGATCGTATCCGTCACCCAGCACATCTCCATCCCTCCCACATGATGATAAGCTCTATGGGCTGCAGCAGTACCAGTATCCTTCTTATTATCAGCCTTCAGCTTCTGCCAATGGGACATATGGTGCTACTGCTAACAAGACCAATGTCCATGGAGGGAAAACTTCAACCCCTGTTACTGCTGAACATGTCCCTTCGTCAGTTATGAATAAGGGAAGCGCCACCACTATGACCAATGGGTATTCTTCAAATAACAGTGTGTCAAATGATTCTTATCAAAGGGCTGGCTATCCTGCGTATGCTCCCGTGTCAGGATATGCTGGTACTTATGGGACTCAACCATCATACCCTTCAGATCCCTTGCTATTTTCTGATAAGTCCAAGCATGGATCAAAAACCGGATATTCTACGCCAGCCGTGCCTCGCAAAGATTTTTCCTCTCAAAGGAATACATCCCTTTCCCAGCCACTTCCGCAATACTCGGTATGTCCACTCCGTCACTCCTTGATTCTAACCTTTAGACTGGATCTAAGCATTTGCTTACATTTGTTTTGTCCTTTACAACATCAGCTTGATTGCATGACGACTTTTCTTTTGTATGTATAGTTTCCTATCTTAAGCTCACAATTGAATCTATTATACTCAACCATCTCTCTATTTATATATGAAATTTTCATGATATTTGATTCTGGCTATGACTATTGTTGTGGTGATACACGTTTTGATAGTTCGTTGTATTTGTTACCTAATTGACGTAGAGCAGGAAGTTCTATTTTTGTTTCTGCACAAACATTTATGCTTCTATTGGTAGAATGTATTCAAAACTCACCTTAACTATTGTTCCGGGGTTGCAGAACTTGCATGGTTATTCATCTGGATTGGGACCGTATTCTGGATATCCTAATGGGATGTACCCAAGCAACACGATGTACAGCCAATATGGAAACACATACAGAGGTAGGTCTAACTATGGATCTACTTATGGATCAAGAACAGGTTCCTATGATAACAAGTATAGATCCACAAGCTATGGCCGTGGTTTTGACCATGTCAAAAGAAATGTGGATGGCTTTGGTGAGCTGAACAAGGGACCGAGAGGTTCCAATAGTTCTGATGATAAAAATGACAAAAGTCTTGGACCTGTCACATTATTACTCAAGGGACAGGACCTTCCTATCAAGAGTGATGATGATAAAGAAGTGCTTCCAGTTCCTGACAAGCAACAGTACACTGGGGAAGATTTATCTGAGAATTATTCTGATGCAAAGTTTTTTGTCATCAAATCTTATAGCGAGGATGATATTCACAAGAGTATGAAATATAATGTGTGGACAAGTACCCCTAATGGCAACAAGAAGCTAGATGCGGCATATCAGGAAGCCAAGGAGAAGTCTGCTGACTGTCCCATATTTTTGCTATTTTCGGTGAGTTAGCGTCTCGATCCAGTATTTTGTTGGGCATTCCTATATTTTCTCTGTTTTACCAGGCCTCACTTTTTGCCTCATGATTTTTGTTACAGGTCAATACTAGTGGCCAATTTGTTGGTTTAGCTGAGATGGTGAGTCCGGTTGATTTTGACAGGACCGTCGAGTATTGGCAGCAGGACAGGTGGACTGGTTGCTTTTCTGTGAAGTGGCGTATCATTAAGGATATCCCAAATAATGTGTTGAGACACATAACTCTAGAGAACAATGAAAACAAACCTGTTACAAACAGTAGGGACACTCAAGAGGTATTCACTCAGGCTTTTTTCCGATTCCCATTAACCCCACACTCCTTTCAACAAACAATGCTTAACTATATGCTCTTGTGCAGGTCAAGTTTGAGAAAGGTATTGAAATTCTCAAAATTTTCAAGGAGCATTCAAGCAAAACCTGCATCCTGGATGATTTTGCATTTTACGAGGCTCGCGAAAAGACAATTCAGGAGAAAAAAGCTAAGGAACAACTGTCAAAAGAGATGGTATGCACCTTTACAATTACTCATTTCATTTATTTTAACACATCTTAATTGAGGATTACTAAGACACAGTGAAGAACACGCAATTTGATTAACATCATACTGATTAGACATTCCAAATACCTTGTTCAATTACAGGTCAACAAGTCCAATGATTCGGCAACTGTTGGAACTGATGTATTGCCGAAATCTCAGGATGGAACCTTGGTGAATGAATCAGTTGTTCCAGATGCTGCAGGGAAGGGGGAGAAGCTTGTAGTAGCGAATGGACCTACTTCTACTGAACCGCCTGCAGACTCTTCGAACAGCTGCTTAACCAGTGACAGCGTCAAGCAGTCGAGTGCTTAA
Protein sequence

>MS.gene66974.t1

MQNLSLDSAEPRTIAVPEPAKKAGHGQANEVPKTFNPNASYVPNGHPSISYYYGGYDGQGDWNDWNVYQNYVNANGGMAQGVYGDSGSYMYPQGYGYAPYGSYPSPSTSPSLPHDDKLYGLQQYQYPSYYQPSASANGTYGATANKTNVHGGKTSTPVTAEHVPSSVMNKGSATTMTNGYSSNNSVSNDSYQRAGYPAYAPVSGYAGTYGTQPSYPSDPLLFSDKSKHGSKTGYSTPAVPRKDFSSQRNTSLSQPLPQYSNLHGYSSGLGPYSGYPNGMYPSNTMYSQYGNTYRGRSNYGSTYGSRTGSYDNKYRSTSYGRGFDHVKRNVDGFGELNKGPRGSNSSDDKNDKSLGPVTLLLKGQDLPIKSDDDKEVLPVPDKQQYTGEDLSENYSDAKFFVIKSYSEDDIHKSMKYNVWTSTPNGNKKLDAAYQEAKEKSADCPIFLLFSVNTSGQFVGLAEMVSPVDFDRTVEYWQQDRWTGCFSVKWRIIKDIPNNVLRHITLENNENKPVTNSRDTQEVKFEKGIEILKIFKEHSSKTCILDDFAFYEAREKTIQEKKAKEQLSKEMVNKSNDSATVGTDVLPKSQDGTLVNESVVPDAAGKGEKLVVANGPTSTEPPADSSNSCLTSDSVKQSSA