Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67991.t1 | XP_028762943.1 | 62.8 | 94 | 29 | 1 | 12 | 99 | 255 | 348 | 7.70E-26 | 126.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67991.t1 | A0A392P9H0 | 63.3 | 90 | 32 | 1 | 2 | 91 | 107 | 195 | 1.4e-24 | 121.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene67991 | 0.808035 | 3.77E-50 | -1.69E-46 |
| MS.gene050221 | MS.gene67991 | 0.803187 | 3.93E-49 | -1.69E-46 |
| MS.gene051744 | MS.gene67991 | 0.801338 | 9.44E-49 | -1.69E-46 |
| MS.gene053974 | MS.gene67991 | 0.811101 | 8.28E-51 | -1.69E-46 |
| MS.gene054236 | MS.gene67991 | 0.812536 | 4.04E-51 | -1.69E-46 |
| MS.gene054651 | MS.gene67991 | 0.808102 | 3.65E-50 | -1.69E-46 |
| MS.gene056050 | MS.gene67991 | 0.807983 | 3.87E-50 | -1.69E-46 |
| MS.gene056854 | MS.gene67991 | 0.809556 | 1.79E-50 | -1.69E-46 |
| MS.gene058037 | MS.gene67991 | 0.808675 | 2.76E-50 | -1.69E-46 |
| MS.gene058515 | MS.gene67991 | 0.81636 | 5.76E-52 | -1.69E-46 |
| MS.gene059272 | MS.gene67991 | 0.843137 | 1.69E-58 | -1.69E-46 |
| MS.gene059276 | MS.gene67991 | 0.811739 | 6.02E-51 | -1.69E-46 |
| MS.gene059584 | MS.gene67991 | 0.803932 | 2.75E-49 | -1.69E-46 |
| MS.gene060167 | MS.gene67991 | 0.812645 | 3.82E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene67991.t1 | MTR_1g036690 | 57.317 | 82 | 28 | 3 | 19 | 99 | 5 | 80 | 1.00e-24 | 89.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACTTCAAAATAAGGATAAA+AGG | 0.234592 | 6.4:-11303569 | MS.gene67991:CDS |
| CCAACAATCACTTCAAAATA+AGG | 0.244294 | 6.4:-11303577 | MS.gene67991:CDS |
| TATAGGTGGGGTTCTATTCC+TGG | 0.331534 | 6.4:+11303415 | None:intergenic |
| TAAAGATCAACAAAATGTTT+TGG | 0.334214 | 6.4:+11303600 | None:intergenic |
| TGAAGATGATTATGGTAATA+TGG | 0.338642 | 6.4:-11303517 | MS.gene67991:CDS |
| GAAAATGATGAAGATGATTA+TGG | 0.391347 | 6.4:-11303525 | MS.gene67991:CDS |
| TGCTATCGTTGTGGAGGAAA+AGG | 0.414829 | 6.4:-11303648 | MS.gene67991:CDS |
| AGAAATCAGCAGCATCTAAG+TGG | 0.464577 | 6.4:+11303487 | None:intergenic |
| AATGTTGTAAAGAATGATAT+TGG | 0.471241 | 6.4:-11303699 | MS.gene67991:CDS |
| ACTTCAAAATAAGGATAAAA+GGG | 0.487291 | 6.4:-11303568 | MS.gene67991:CDS |
| GTTGTGGAGGAAAAGGTCAT+TGG | 0.504176 | 6.4:-11303641 | MS.gene67991:CDS |
| CATTAGAGATGAAAATATCC+AGG | 0.532865 | 6.4:-11303433 | MS.gene67991:CDS |
| GAGAGTATTTGCTATCGTTG+TGG | 0.583351 | 6.4:-11303657 | MS.gene67991:CDS |
| AGTATTTGCTATCGTTGTGG+AGG | 0.599695 | 6.4:-11303654 | MS.gene67991:CDS |
| GAAATCAGCAGCATCTAAGT+GGG | 0.621669 | 6.4:+11303488 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGTTGTAAAGAATGATAT+TGG | - | chr6.4:11303434-11303453 | MS.gene67991:CDS | 20.0% |
| !! | ACTTCAAAATAAGGATAAAA+GGG | - | chr6.4:11303565-11303584 | MS.gene67991:CDS | 20.0% |
| !!! | TAAAGATCAACAAAATGTTT+TGG | + | chr6.4:11303536-11303555 | None:intergenic | 20.0% |
| ! | CACTTCAAAATAAGGATAAA+AGG | - | chr6.4:11303564-11303583 | MS.gene67991:CDS | 25.0% |
| ! | GAAAATGATGAAGATGATTA+TGG | - | chr6.4:11303608-11303627 | MS.gene67991:CDS | 25.0% |
| ! | TGAAGATGATTATGGTAATA+TGG | - | chr6.4:11303616-11303635 | MS.gene67991:CDS | 25.0% |
| !! | TATTTTCATCTCTAATGAGA+TGG | + | chr6.4:11303697-11303716 | None:intergenic | 25.0% |
| CATTAGAGATGAAAATATCC+AGG | - | chr6.4:11303700-11303719 | MS.gene67991:CDS | 30.0% | |
| CCAACAATCACTTCAAAATA+AGG | - | chr6.4:11303556-11303575 | MS.gene67991:CDS | 30.0% | |
| !!! | CCTTATTTTGAAGTGATTGT+TGG | + | chr6.4:11303559-11303578 | None:intergenic | 30.0% |
| ! | AGATGGTCAATCTTTTCATC+AGG | + | chr6.4:11303680-11303699 | None:intergenic | 35.0% |
| AGAAATCAGCAGCATCTAAG+TGG | + | chr6.4:11303649-11303668 | None:intergenic | 40.0% | |
| GAAATCAGCAGCATCTAAGT+GGG | + | chr6.4:11303648-11303667 | None:intergenic | 40.0% | |
| !! | AGTATTTGCTATCGTTGTGG+AGG | - | chr6.4:11303479-11303498 | MS.gene67991:CDS | 40.0% |
| !! | GAGAGTATTTGCTATCGTTG+TGG | - | chr6.4:11303476-11303495 | MS.gene67991:CDS | 40.0% |
| !!! | AAAATGTTTTGGCGTACGAC+AGG | + | chr6.4:11303525-11303544 | None:intergenic | 40.0% |
| GTTGTGGAGGAAAAGGTCAT+TGG | - | chr6.4:11303492-11303511 | MS.gene67991:CDS | 45.0% | |
| TGCTATCGTTGTGGAGGAAA+AGG | - | chr6.4:11303485-11303504 | MS.gene67991:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 11303428 | 11303727 | 11303428 | ID=MS.gene67991 |
| chr6.4 | mRNA | 11303428 | 11303727 | 11303428 | ID=MS.gene67991.t1;Parent=MS.gene67991 |
| chr6.4 | exon | 11303428 | 11303727 | 11303428 | ID=MS.gene67991.t1.exon1;Parent=MS.gene67991.t1 |
| chr6.4 | CDS | 11303428 | 11303727 | 11303428 | ID=cds.MS.gene67991.t1;Parent=MS.gene67991.t1 |
| Gene Sequence |
| Protein sequence |
