Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68532.t1 | XP_013459971.1 | 65.1 | 126 | 30 | 1 | 1 | 112 | 77 | 202 | 1.00E-32 | 149.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68532.t1 | A0A072UWM6 | 65.1 | 126 | 30 | 1 | 1 | 112 | 77 | 202 | 7.5e-33 | 149.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049159 | MS.gene68532 | 0.846468 | 2.13E-59 | -1.69E-46 |
| MS.gene050807 | MS.gene68532 | 0.800927 | 1.15E-48 | -1.69E-46 |
| MS.gene051180 | MS.gene68532 | 0.811375 | 7.22E-51 | -1.69E-46 |
| MS.gene051198 | MS.gene68532 | 0.810967 | 8.86E-51 | -1.69E-46 |
| MS.gene052516 | MS.gene68532 | 0.808498 | 3.01E-50 | -1.69E-46 |
| MS.gene052665 | MS.gene68532 | 0.801496 | 8.76E-49 | -1.69E-46 |
| MS.gene053008 | MS.gene68532 | 0.836629 | 8.37E-57 | -1.69E-46 |
| MS.gene057446 | MS.gene68532 | 0.806141 | 9.50E-50 | -1.69E-46 |
| MS.gene057869 | MS.gene68532 | 0.812129 | 4.95E-51 | -1.69E-46 |
| MS.gene057915 | MS.gene68532 | 0.806164 | 9.40E-50 | -1.69E-46 |
| MS.gene058010 | MS.gene68532 | 0.8089 | 2.47E-50 | -1.69E-46 |
| MS.gene059713 | MS.gene68532 | 0.801264 | 9.77E-49 | -1.69E-46 |
| MS.gene059725 | MS.gene68532 | 0.805159 | 1.53E-49 | -1.69E-46 |
| MS.gene059842 | MS.gene68532 | 0.812254 | 4.65E-51 | -1.69E-46 |
| MS.gene059937 | MS.gene68532 | 0.802878 | 4.55E-49 | -1.69E-46 |
| MS.gene060803 | MS.gene68532 | 0.816448 | 5.50E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68532.t1 | MTR_3g056730 | 62.698 | 126 | 33 | 1 | 1 | 112 | 77 | 202 | 1.25e-39 | 139 |
| MS.gene68532.t1 | MTR_3g056730 | 68.182 | 44 | 14 | 0 | 69 | 112 | 248 | 291 | 2.94e-12 | 62.4 |
| MS.gene68532.t1 | MTR_3g057330 | 51.948 | 77 | 24 | 2 | 4 | 69 | 85 | 159 | 6.83e-12 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 39 sgRNAs with CRISPR-Local
Find 88 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATGGAGTAATGGGGAATT+AGG | 0.175752 | 3.4:-54228082 | MS.gene68532:CDS |
| AAAGATGGTGTAGGTGCTTC+AGG | 0.318345 | 3.4:-54227953 | MS.gene68532:intron |
| TTCAGGAAGAATGGAGTAAT+GGG | 0.327280 | 3.4:-54228090 | MS.gene68532:CDS |
| AGGTGAAATTCAAAAGTTAA+AGG | 0.347607 | 3.4:-54226912 | None:intergenic |
| AATGGAGTAATGGGGAATTA+GGG | 0.360804 | 3.4:-54228081 | MS.gene68532:CDS |
| GAATGGTCAATTCACCAATT+GGG | 0.388247 | 3.4:-54227624 | MS.gene68532:CDS |
| TCGACGAGACGCTAGTGGTT+AGG | 0.389742 | 3.4:-54227697 | MS.gene68532:CDS |
| CGACGAGACGCTAGTGGTTA+GGG | 0.409049 | 3.4:-54227696 | MS.gene68532:CDS |
| AGTAATGGGGAATTAGGGTT+TGG | 0.420812 | 3.4:-54228076 | MS.gene68532:CDS |
| AGAATGGTCAATTCACCAAT+TGG | 0.432671 | 3.4:-54227625 | MS.gene68532:CDS |
| GAAATTTGATATGTTGATGT+TGG | 0.433409 | 3.4:-54228146 | None:intergenic |
| AATGGAGCTGAAGAGAGTTC+AGG | 0.434303 | 3.4:-54228107 | MS.gene68532:CDS |
| CCATTCCCTCACCTTCTCTT+CGG | 0.443552 | 3.4:+54227652 | None:intergenic |
| AAAATTGAGACTTCCCCAAT+TGG | 0.449906 | 3.4:+54227610 | None:intergenic |
| AGAAAGAAGTTCAAGAGCTT+AGG | 0.460855 | 3.4:-54226937 | MS.gene68532:intron |
| GGAAAAGGTTAGTGCTGAAA+TGG | 0.469665 | 3.4:-54228125 | MS.gene68532:CDS |
| TGAAGAGAGTTCAGGAAGAA+TGG | 0.480654 | 3.4:-54228099 | MS.gene68532:CDS |
| TGATATGTTGATGTTGGAAA+AGG | 0.480946 | 3.4:-54228140 | None:intergenic |
| ATGAAAGAAAAGAGAGGGGT+TGG | 0.485623 | 3.4:-54227989 | MS.gene68532:CDS |
| GTTCAGGAAGAATGGAGTAA+TGG | 0.487063 | 3.4:-54228091 | MS.gene68532:CDS |
| TTAGGGTTTGGAAGGTGTAG+TGG | 0.505005 | 3.4:-54228064 | MS.gene68532:CDS |
| CAGCGGCCGAAGAGAAGGTG+AGG | 0.533662 | 3.4:-54227658 | MS.gene68532:CDS |
| GTGAGGGAATGGCAAGAGAA+TGG | 0.536093 | 3.4:-54227641 | MS.gene68532:CDS |
| GAAGGCAGCGGCCGAAGAGA+AGG | 0.550222 | 3.4:-54227663 | MS.gene68532:CDS |
| ATGGGGAATTAGGGTTTGGA+AGG | 0.550708 | 3.4:-54228072 | MS.gene68532:CDS |
| GGTTAGGGAAGCAATGTTGA+AGG | 0.552460 | 3.4:-54227681 | MS.gene68532:CDS |
| CCGAAGAGAAGGTGAGGGAA+TGG | 0.555436 | 3.4:-54227652 | MS.gene68532:CDS |
| GGAAGCAATGTTGAAGGCAG+CGG | 0.562483 | 3.4:-54227675 | MS.gene68532:CDS |
| TTTGATGAAAGAAAAGAGAG+GGG | 0.576044 | 3.4:-54227993 | MS.gene68532:CDS |
| GTTTGGAAGGTGTAGTGGAG+AGG | 0.583748 | 3.4:-54228059 | MS.gene68532:CDS |
| AATGGTCAATTCACCAATTG+GGG | 0.591237 | 3.4:-54227623 | MS.gene68532:CDS |
| AGCGGCCGAAGAGAAGGTGA+GGG | 0.599801 | 3.4:-54227657 | MS.gene68532:CDS |
| CCACTAGCGTCTCGTCGACA+CGG | 0.606487 | 3.4:+54227702 | None:intergenic |
| TCAGGAAGAATGGAGTAATG+GGG | 0.611310 | 3.4:-54228089 | MS.gene68532:CDS |
| GGTGAACAAAGCAAGAAAGA+TGG | 0.617411 | 3.4:-54227968 | MS.gene68532:CDS |
| CAAAGCAAGAAAGATGGTGT+AGG | 0.629742 | 3.4:-54227962 | MS.gene68532:CDS |
| GAAGTTCAAGAGCTTAGGAG+AGG | 0.636482 | 3.4:-54226932 | MS.gene68532:intron |
| CCGTGTCGACGAGACGCTAG+TGG | 0.641306 | 3.4:-54227702 | MS.gene68532:CDS |
| AGAGGTGCTGAAGAAGACAA+AGG | 0.713146 | 3.4:-54228041 | MS.gene68532:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATTTTTAGTGTTTTAAA+AGG | - | chr3.4:54227798-54227817 | MS.gene68532:intron | 10.0% |
| !!! | ATATTTTTAGTGTTTTAAAA+GGG | - | chr3.4:54227799-54227818 | MS.gene68532:intron | 10.0% |
| !! | ATTAGCAGTTAAATTATATT+TGG | - | chr3.4:54227272-54227291 | MS.gene68532:intron | 15.0% |
| !! | TAAATAAGATTAAACCTTAA+TGG | + | chr3.4:54227957-54227976 | None:intergenic | 15.0% |
| !!! | AAAATAATTTTAGCGAAAAA+CGG | + | chr3.4:54227624-54227643 | None:intergenic | 15.0% |
| !!! | TAAAATAGTCATTTTGTTAA+AGG | - | chr3.4:54227773-54227792 | MS.gene68532:intron | 15.0% |
| !!! | TATTCAGAAATAGTTAATTT+TGG | - | chr3.4:54227687-54227706 | MS.gene68532:CDS | 15.0% |
| !!! | TTTTTAATGTTTAAACCAAA+TGG | - | chr3.4:54227302-54227321 | MS.gene68532:intron | 15.0% |
| !!! | TTTTTTTTTTTTCTCTTAAG+AGG | + | chr3.4:54228007-54228026 | None:intergenic | 15.0% |
| !! | ATAACAATTGTCATAAGAAT+AGG | - | chr3.4:54228049-54228068 | MS.gene68532:CDS | 20.0% |
| !! | ATTACTAAAATACCTTTCTT+AGG | + | chr3.4:54227713-54227732 | None:intergenic | 20.0% |
| !! | TAACAATTGTCATAAGAATA+GGG | - | chr3.4:54228050-54228069 | MS.gene68532:CDS | 20.0% |
| !! | TATTATTGAGTATGTATGTT+TGG | - | chr3.4:54227173-54227192 | MS.gene68532:intron | 20.0% |
| !!! | AAATAATTTTAGCGAAAAAC+GGG | + | chr3.4:54227623-54227642 | None:intergenic | 20.0% |
| !!! | ATTTTAGTAATTTTAGACTC+AGG | - | chr3.4:54227722-54227741 | MS.gene68532:intron | 20.0% |
| !!! | TTTTAGTAATTTTAGACTCA+GGG | - | chr3.4:54227723-54227742 | MS.gene68532:intron | 20.0% |
| !!! | TTTTATATACACCACATATT+GGG | + | chr3.4:54227981-54228000 | None:intergenic | 20.0% |
| ! | GTTTAAACCAAATGGAATTA+CGG | - | chr3.4:54227310-54227329 | MS.gene68532:intron | 25.0% |
| ! | TTCTAATTTACTAACGAGTT+GGG | - | chr3.4:54227839-54227858 | MS.gene68532:intron | 25.0% |
| ! | TTTCTAATTTACTAACGAGT+TGG | - | chr3.4:54227838-54227857 | MS.gene68532:intron | 25.0% |
| !! | GTTTTATATACACCACATAT+TGG | + | chr3.4:54227982-54228001 | None:intergenic | 25.0% |
| !! | TTTAGTAATTTTAGACTCAG+GGG | - | chr3.4:54227724-54227743 | MS.gene68532:intron | 25.0% |
| !! | TTTTGATGAAAGAAAAGAGA+GGG | - | chr3.4:54227057-54227076 | MS.gene68532:intron | 25.0% |
| !!! | TTTAGTGACATAAGTTTTAG+AGG | - | chr3.4:54227931-54227950 | MS.gene68532:intron | 25.0% |
| TCTAATTTACTAACGAGTTG+GGG | - | chr3.4:54227840-54227859 | MS.gene68532:intron | 30.0% | |
| TGGGGTAATTATTTAAGAGA+CGG | - | chr3.4:54227858-54227877 | MS.gene68532:intron | 30.0% | |
| TTCAGAAAACTCAACTTGTA+AGG | + | chr3.4:54227560-54227579 | None:intergenic | 30.0% | |
| TTTGATGAAAGAAAAGAGAG+GGG | - | chr3.4:54227058-54227077 | MS.gene68532:intron | 30.0% | |
| ! | ATCTTATTTAGCCCAATATG+TGG | - | chr3.4:54227967-54227986 | MS.gene68532:CDS | 30.0% |
| !! | AGTTAATTTTGGCCTAAGAA+AGG | - | chr3.4:54227698-54227717 | MS.gene68532:CDS | 30.0% |
| !! | GTAATTATCCGATGTTTTGA+CGG | - | chr3.4:54227880-54227899 | MS.gene68532:intron | 30.0% |
| !! | GTTTTGATGAAAGAAAAGAG+AGG | - | chr3.4:54227056-54227075 | MS.gene68532:intron | 30.0% |
| !!! | ACAAAGGTTGTTGATTTTGA+AGG | - | chr3.4:54227026-54227045 | MS.gene68532:intron | 30.0% |
| !!! | ATAAGTTTTAGAGGCCATTA+AGG | - | chr3.4:54227940-54227959 | MS.gene68532:intron | 30.0% |
| AAAATTGAGACTTCCCCAAT+TGG | + | chr3.4:54227444-54227463 | None:intergenic | 35.0% | |
| AATGGTCAATTCACCAATTG+GGG | - | chr3.4:54227428-54227447 | MS.gene68532:intron | 35.0% | |
| AGAAAGAAGTTCAAGAGCTT+AGG | - | chr3.4:54228114-54228133 | MS.gene68532:CDS | 35.0% | |
| AGAATGGTCAATTCACCAAT+TGG | - | chr3.4:54227426-54227445 | MS.gene68532:intron | 35.0% | |
| ATTGGTCATTTGATGTGCAA+AGG | - | chr3.4:54227750-54227769 | MS.gene68532:intron | 35.0% | |
| CAATTGTCATAAGAATAGGG+AGG | - | chr3.4:54228053-54228072 | MS.gene68532:CDS | 35.0% | |
| CACTTAAACCGTCAAAACAT+CGG | + | chr3.4:54227891-54227910 | None:intergenic | 35.0% | |
| CATTCGACAATGAGAAAGTT+CGG | + | chr3.4:54227594-54227613 | None:intergenic | 35.0% | |
| CTCTTGAACTTCTTTCTTCT+AGG | + | chr3.4:54228111-54228130 | None:intergenic | 35.0% | |
| GAATGGTCAATTCACCAATT+GGG | - | chr3.4:54227427-54227446 | MS.gene68532:intron | 35.0% | |
| TTCAGGAAGAATGGAGTAAT+GGG | - | chr3.4:54226961-54226980 | MS.gene68532:intron | 35.0% | |
| ! | AATGGAGTAATGGGGAATTA+GGG | - | chr3.4:54226970-54226989 | MS.gene68532:intron | 35.0% |
| ! | TTCTAGAAATGACAGTTGCT+TGG | - | chr3.4:54227490-54227509 | MS.gene68532:intron | 35.0% |
| !! | GTTGAGTTTTCTGAAAGTTG+TGG | - | chr3.4:54227565-54227584 | MS.gene68532:intron | 35.0% |
| !! | TTTTAGACTCAGGGGTATAT+TGG | - | chr3.4:54227732-54227751 | MS.gene68532:intron | 35.0% |
| AAACCAAATGGAATTACGGC+AGG | - | chr3.4:54227314-54227333 | MS.gene68532:intron | 40.0% | |
| AACCAAATGGAATTACGGCA+GGG | - | chr3.4:54227315-54227334 | MS.gene68532:intron | 40.0% | |
| ATGAAAGAAAAGAGAGGGGT+TGG | - | chr3.4:54227062-54227081 | MS.gene68532:intron | 40.0% | |
| CAAAGCAAGAAAGATGGTGT+AGG | - | chr3.4:54227089-54227108 | MS.gene68532:intron | 40.0% | |
| GGTGAACAAAGCAAGAAAGA+TGG | - | chr3.4:54227083-54227102 | MS.gene68532:intron | 40.0% | |
| GTTCAGGAAGAATGGAGTAA+TGG | - | chr3.4:54226960-54226979 | MS.gene68532:intron | 40.0% | |
| TCAGGAAGAATGGAGTAATG+GGG | - | chr3.4:54226962-54226981 | MS.gene68532:intron | 40.0% | |
| TGAAGAGAGTTCAGGAAGAA+TGG | - | chr3.4:54226952-54226971 | MS.gene68532:intron | 40.0% | |
| ! | AGTAATGGGGAATTAGGGTT+TGG | - | chr3.4:54226975-54226994 | MS.gene68532:intron | 40.0% |
| ! | GAATGGAGTAATGGGGAATT+AGG | - | chr3.4:54226969-54226988 | MS.gene68532:intron | 40.0% |
| ! | GGAAAAGGTTAGTGCTGAAA+TGG | - | chr3.4:54226926-54226945 | MS.gene68532:intron | 40.0% |
| ! | TTGGGGAAGTCTCAATTTTG+AGG | - | chr3.4:54227445-54227464 | MS.gene68532:intron | 40.0% |
| AAAGATGGTGTAGGTGCTTC+AGG | - | chr3.4:54227098-54227117 | MS.gene68532:intron | 45.0% | |
| AAGAATAGGGAGGTAGAGAG+AGG | - | chr3.4:54228063-54228082 | MS.gene68532:CDS | 45.0% | |
| AATGGAGCTGAAGAGAGTTC+AGG | - | chr3.4:54226944-54226963 | MS.gene68532:intron | 45.0% | |
| ACCAAATGGAATTACGGCAG+GGG | - | chr3.4:54227316-54227335 | MS.gene68532:intron | 45.0% | |
| AGAATAGGGAGGTAGAGAGA+GGG | - | chr3.4:54228064-54228083 | MS.gene68532:CDS | 45.0% | |
| ATGGGGAATTAGGGTTTGGA+AGG | - | chr3.4:54226979-54226998 | MS.gene68532:intron | 45.0% | |
| GAAGTTCAAGAGCTTAGGAG+AGG | - | chr3.4:54228119-54228138 | MS.gene68532:CDS | 45.0% | |
| GGTTAGGGAAGCAATGTTGA+AGG | - | chr3.4:54227370-54227389 | MS.gene68532:intron | 45.0% | |
| ! | TTAGGGTTTGGAAGGTGTAG+TGG | - | chr3.4:54226987-54227006 | MS.gene68532:intron | 45.0% |
| !! | AGAGGTGCTGAAGAAGACAA+AGG | - | chr3.4:54227010-54227029 | MS.gene68532:intron | 45.0% |
| !! | TAAAATTTAATAACAAAAAT+TGG | - | chr3.4:54227527-54227546 | MS.gene68532:intron | 5.0% |
| AGAGGGAGAGTAGGAACATG+AGG | - | chr3.4:54228081-54228100 | MS.gene68532:CDS | 50.0% | |
| CCATTCCCTCACCTTCTCTT+CGG | + | chr3.4:54227402-54227421 | None:intergenic | 50.0% | |
| GGAAGCAATGTTGAAGGCAG+CGG | - | chr3.4:54227376-54227395 | MS.gene68532:intron | 50.0% | |
| GTGAGGGAATGGCAAGAGAA+TGG | - | chr3.4:54227410-54227429 | MS.gene68532:intron | 50.0% | |
| TCCCCTGCCGTAATTCCATT+TGG | + | chr3.4:54227320-54227339 | None:intergenic | 50.0% | |
| TGGTGTAGGTGCTTCAGGTA+TGG | - | chr3.4:54227103-54227122 | MS.gene68532:intron | 50.0% | |
| ! | GTTTGGAAGGTGTAGTGGAG+AGG | - | chr3.4:54226992-54227011 | MS.gene68532:intron | 50.0% |
| CCGAAGAGAAGGTGAGGGAA+TGG | - | chr3.4:54227399-54227418 | MS.gene68532:intron | 55.0% | |
| CGACGAGACGCTAGTGGTTA+GGG | - | chr3.4:54227355-54227374 | MS.gene68532:intron | 55.0% | |
| GAGGTAGAGAGAGGGAGAGT+AGG | - | chr3.4:54228072-54228091 | MS.gene68532:CDS | 55.0% | |
| TCGACGAGACGCTAGTGGTT+AGG | - | chr3.4:54227354-54227373 | MS.gene68532:intron | 55.0% | |
| AGCGGCCGAAGAGAAGGTGA+GGG | - | chr3.4:54227394-54227413 | MS.gene68532:intron | 60.0% | |
| CCACTAGCGTCTCGTCGACA+CGG | + | chr3.4:54227352-54227371 | None:intergenic | 60.0% | |
| CAGCGGCCGAAGAGAAGGTG+AGG | - | chr3.4:54227393-54227412 | MS.gene68532:intron | 65.0% | |
| GAAGGCAGCGGCCGAAGAGA+AGG | - | chr3.4:54227388-54227407 | MS.gene68532:intron | 65.0% | |
| ! | CCGTGTCGACGAGACGCTAG+TGG | - | chr3.4:54227349-54227368 | MS.gene68532:intron | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 54226915 | 54228158 | 54226915 | ID=MS.gene68532 |
| chr3.4 | mRNA | 54226915 | 54228158 | 54226915 | ID=MS.gene68532.t1;Parent=MS.gene68532 |
| chr3.4 | exon | 54227954 | 54228158 | 54227954 | ID=MS.gene68532.t1.exon1;Parent=MS.gene68532.t1 |
| chr3.4 | CDS | 54227954 | 54228158 | 54227954 | ID=cds.MS.gene68532.t1;Parent=MS.gene68532.t1 |
| chr3.4 | exon | 54227607 | 54227737 | 54227607 | ID=MS.gene68532.t1.exon2;Parent=MS.gene68532.t1 |
| chr3.4 | CDS | 54227607 | 54227737 | 54227607 | ID=cds.MS.gene68532.t1;Parent=MS.gene68532.t1 |
| chr3.4 | exon | 54226915 | 54226944 | 54226915 | ID=MS.gene68532.t1.exon3;Parent=MS.gene68532.t1 |
| chr3.4 | CDS | 54226915 | 54226944 | 54226915 | ID=cds.MS.gene68532.t1;Parent=MS.gene68532.t1 |
| Gene Sequence |
| Protein sequence |
