Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68949.t1 | XP_003610465.2 | 97.9 | 233 | 5 | 0 | 1 | 233 | 102 | 334 | 6.70E-137 | 496.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68949.t1 | A0A0C3X8T8 | 97.9 | 233 | 5 | 0 | 1 | 233 | 102 | 334 | 4.8e-137 | 496.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049418 | MS.gene68949 | 0.822949 | 1.80E-53 | -1.69E-46 |
| MS.gene049711 | MS.gene68949 | 0.801546 | 8.55E-49 | -1.69E-46 |
| MS.gene051018 | MS.gene68949 | 0.83857 | 2.66E-57 | -1.69E-46 |
| MS.gene051478 | MS.gene68949 | 0.810091 | 1.37E-50 | -1.69E-46 |
| MS.gene051720 | MS.gene68949 | 0.816251 | 6.09E-52 | -1.69E-46 |
| MS.gene053144 | MS.gene68949 | 0.828946 | 6.77E-55 | -1.69E-46 |
| MS.gene053538 | MS.gene68949 | 0.802652 | 5.07E-49 | -1.69E-46 |
| MS.gene054584 | MS.gene68949 | 0.832097 | 1.15E-55 | -1.69E-46 |
| MS.gene055465 | MS.gene68949 | 0.80501 | 1.64E-49 | -1.69E-46 |
| MS.gene055466 | MS.gene68949 | 0.805975 | 1.03E-49 | -1.69E-46 |
| MS.gene055537 | MS.gene68949 | 0.825329 | 4.97E-54 | -1.69E-46 |
| MS.gene056169 | MS.gene68949 | 0.804142 | 2.49E-49 | -1.69E-46 |
| MS.gene058909 | MS.gene68949 | 0.803964 | 2.71E-49 | -1.69E-46 |
| MS.gene058912 | MS.gene68949 | 0.80005 | 1.73E-48 | -1.69E-46 |
| MS.gene060519 | MS.gene68949 | 0.81616 | 6.38E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68949.t1 | MTR_4g132450 | 97.854 | 233 | 5 | 0 | 1 | 233 | 102 | 334 | 7.54e-175 | 484 |
| MS.gene68949.t1 | MTR_2g023970 | 78.112 | 233 | 50 | 1 | 1 | 233 | 89 | 320 | 7.71e-133 | 377 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene68949.t1 | AT1G11915 | 64.831 | 236 | 78 | 5 | 1 | 233 | 96 | 329 | 4.30e-95 | 281 |
Find 39 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTACTGCTATTTCAGCTTT+TGG | 0.228291 | 8.2:-1671144 | MS.gene68949:CDS |
| TTGGAATTGGAGGTGGTATT+TGG | 0.228294 | 8.2:-1671116 | MS.gene68949:CDS |
| CAAGTTATGCTAGTGTTTAT+TGG | 0.239276 | 8.2:-1671854 | MS.gene68949:CDS |
| TATGATCAAGTTCCTCAATA+TGG | 0.240179 | 8.2:-1671807 | MS.gene68949:CDS |
| CTTTGAACTCCACATGTTAC+TGG | 0.265726 | 8.2:+1671066 | None:intergenic |
| CTCAAAGATTCAGTAGCTTT+AGG | 0.294315 | 8.2:+1671897 | None:intergenic |
| ACAAGTTCCACCACCACCTT+TGG | 0.367403 | 8.2:+1671725 | None:intergenic |
| TGTTCACAAAACACAGGCTT+TGG | 0.373458 | 8.2:+1672026 | None:intergenic |
| CAAGATGAATCACAGTGTTC+AGG | 0.400798 | 8.2:+1671999 | None:intergenic |
| GTGCTTGTGTCTAAACTAAA+TGG | 0.403773 | 8.2:+1672119 | None:intergenic |
| AGGAATGTCCATGTAATTGC+AGG | 0.403998 | 8.2:-1671296 | MS.gene68949:intron |
| CTAAAGCTACTGAATCTTTG+AGG | 0.411355 | 8.2:-1671896 | MS.gene68949:CDS |
| ATGATCAAGTTCCTCAATAT+GGG | 0.412810 | 8.2:-1671806 | MS.gene68949:CDS |
| TTCAATCACCTGCAATTACA+TGG | 0.419145 | 8.2:+1671288 | None:intergenic |
| TGAAGACACCGCGTCGTCAC+TGG | 0.426939 | 8.2:+1671762 | None:intergenic |
| GAGTTTCTTGTGTTTATGTA+AGG | 0.427897 | 8.2:-1671682 | MS.gene68949:CDS |
| TATTGAGGAACTTGATCATA+AGG | 0.446946 | 8.2:+1671810 | None:intergenic |
| TTTGGAGCACTTGGAATTGG+AGG | 0.470180 | 8.2:-1671126 | MS.gene68949:CDS |
| AATTTCACTACTCATTGTAA+TGG | 0.476094 | 8.2:-1671654 | MS.gene68949:intron |
| TCTAAACTAAATGGTCTTGT+TGG | 0.485018 | 8.2:+1672128 | None:intergenic |
| GACGCGGTGTCTTCAATGTC+AGG | 0.485220 | 8.2:-1671754 | MS.gene68949:CDS |
| GAGTTCAAAGCAATGAGAAC+AGG | 0.485646 | 8.2:-1671053 | MS.gene68949:CDS |
| AGTGTTTATTGGAGAAATGT+TGG | 0.491654 | 8.2:-1671843 | MS.gene68949:CDS |
| TTCTTGTGTTTATGTAAGGA+AGG | 0.498390 | 8.2:-1671678 | MS.gene68949:CDS |
| GATCAATCCAAAGGTGGTGG+TGG | 0.501256 | 8.2:-1671732 | MS.gene68949:CDS |
| ATGATAATGCAAGGCACAGT+AGG | 0.505376 | 8.2:-1671316 | MS.gene68949:CDS |
| GGAGCACTTGGAATTGGAGG+TGG | 0.523403 | 8.2:-1671123 | MS.gene68949:CDS |
| AAAGGTGGTGGTGGAACTTG+TGG | 0.581771 | 8.2:-1671723 | MS.gene68949:CDS |
| CAGGATCAATCCAAAGGTGG+TGG | 0.583668 | 8.2:-1671735 | MS.gene68949:CDS |
| TCAACTCTAATCCCATATTG+AGG | 0.585217 | 8.2:+1671795 | None:intergenic |
| GGAAATTGTTCACAAAACAC+AGG | 0.597733 | 8.2:+1672020 | None:intergenic |
| TTGATATTCCAGTGACGACG+CGG | 0.620201 | 8.2:-1671770 | MS.gene68949:CDS |
| TGGCAGATCATGATAATGCA+AGG | 0.621886 | 8.2:-1671325 | MS.gene68949:intron |
| GGAACTTGATCATAAGGCTG+AGG | 0.624162 | 8.2:+1671816 | None:intergenic |
| GCTAAAGAACCAGTAACATG+TGG | 0.630947 | 8.2:-1671075 | MS.gene68949:CDS |
| CTCATAGCAAAAGAACACTC+TGG | 0.643337 | 8.2:+1671945 | None:intergenic |
| CAATGTCAGGATCAATCCAA+AGG | 0.643953 | 8.2:-1671741 | MS.gene68949:CDS |
| TTCATGTGGAACTGTGAAGA+CGG | 0.666043 | 8.2:-1672164 | MS.gene68949:CDS |
| TGTCAGGATCAATCCAAAGG+TGG | 0.681857 | 8.2:-1671738 | MS.gene68949:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGATTTTGAATTAATTTAAT+TGG | - | chr8.2:1671843-1671862 | MS.gene68949:CDS | 10.0% |
| !! | AATTGGATTTATTATTTGTT+TGG | - | chr8.2:1671860-1671879 | MS.gene68949:CDS | 15.0% |
| !!! | TACTTTTTTAATCATGTTAT+TGG | - | chr8.2:1671639-1671658 | MS.gene68949:intron | 15.0% |
| !!! | AACTATTGAGTAACAATTTT+TGG | - | chr8.2:1671819-1671838 | MS.gene68949:CDS | 20.0% |
| ! | AATTAACATTCACAAAGTCA+GGG | - | chr8.2:1671963-1671982 | MS.gene68949:CDS | 25.0% |
| ! | AATTTCACTACTCATTGTAA+TGG | - | chr8.2:1671551-1671570 | MS.gene68949:intron | 25.0% |
| ! | TAATTAACATTCACAAAGTC+AGG | - | chr8.2:1671962-1671981 | MS.gene68949:CDS | 25.0% |
| ! | TCACAATCACAAGATAAAAT+AGG | + | chr8.2:1671598-1671617 | None:intergenic | 25.0% |
| !!! | GGTTTTTGAAGTTGAAAATT+AGG | - | chr8.2:1671984-1672003 | MS.gene68949:CDS | 25.0% |
| AGTGTTTATTGGAGAAATGT+TGG | - | chr8.2:1671362-1671381 | MS.gene68949:intron | 30.0% | |
| ATGATCAAGTTCCTCAATAT+GGG | - | chr8.2:1671399-1671418 | MS.gene68949:intron | 30.0% | |
| CAAGTTATGCTAGTGTTTAT+TGG | - | chr8.2:1671351-1671370 | MS.gene68949:intron | 30.0% | |
| GAAAATTAGGCTTATGTTTG+TGG | - | chr8.2:1671997-1672016 | MS.gene68949:CDS | 30.0% | |
| GAGTTTCTTGTGTTTATGTA+AGG | - | chr8.2:1671523-1671542 | MS.gene68949:intron | 30.0% | |
| TATGATCAAGTTCCTCAATA+TGG | - | chr8.2:1671398-1671417 | MS.gene68949:intron | 30.0% | |
| TATTGAGGAACTTGATCATA+AGG | + | chr8.2:1671398-1671417 | None:intergenic | 30.0% | |
| TTATCTTGTGATTGTGACTT+AGG | - | chr8.2:1671601-1671620 | MS.gene68949:intron | 30.0% | |
| TTCTTGTGTTTATGTAAGGA+AGG | - | chr8.2:1671527-1671546 | MS.gene68949:intron | 30.0% | |
| !! | TCTAAACTAAATGGTCTTGT+TGG | + | chr8.2:1671080-1671099 | None:intergenic | 30.0% |
| ACTTGGATGATTACCTTTAG+TGG | - | chr8.2:1671678-1671697 | MS.gene68949:CDS | 35.0% | |
| CACTCGTACTAACATAGTAT+TGG | - | chr8.2:1671779-1671798 | MS.gene68949:CDS | 35.0% | |
| CTAAAGCTACTGAATCTTTG+AGG | - | chr8.2:1671309-1671328 | MS.gene68949:CDS | 35.0% | |
| CTCAAAGATTCAGTAGCTTT+AGG | + | chr8.2:1671311-1671330 | None:intergenic | 35.0% | |
| CTTGGATGATTACCTTTAGT+GGG | - | chr8.2:1671679-1671698 | MS.gene68949:CDS | 35.0% | |
| GGAAATTGTTCACAAAACAC+AGG | + | chr8.2:1671188-1671207 | None:intergenic | 35.0% | |
| GTGCTTGTGTCTAAACTAAA+TGG | + | chr8.2:1671089-1671108 | None:intergenic | 35.0% | |
| TATGTTAGTACGAGTGAAAC+TGG | + | chr8.2:1671775-1671794 | None:intergenic | 35.0% | |
| TCAACTCTAATCCCATATTG+AGG | + | chr8.2:1671413-1671432 | None:intergenic | 35.0% | |
| TTCAATCACCTGCAATTACA+TGG | + | chr8.2:1671920-1671939 | None:intergenic | 35.0% | |
| TTGGATGATTACCTTTAGTG+GGG | - | chr8.2:1671680-1671699 | MS.gene68949:CDS | 35.0% | |
| ! | GTTTTGCTTTGTGTTGGATT+TGG | - | chr8.2:1672019-1672038 | MS.gene68949:CDS | 35.0% |
| ! | TTGTGTTGGATTTGGAATTC+TGG | - | chr8.2:1672027-1672046 | MS.gene68949:CDS | 35.0% |
| !! | CGTACTAACATAGTATTGGT+AGG | - | chr8.2:1671783-1671802 | MS.gene68949:CDS | 35.0% |
| !! | TTTGTGGTTTTGCTTTGTGT+TGG | - | chr8.2:1672013-1672032 | MS.gene68949:CDS | 35.0% |
| !!! | GTTACTGCTATTTCAGCTTT+TGG | - | chr8.2:1672061-1672080 | MS.gene68949:CDS | 35.0% |
| AAGTTCCAAAACCCCACTAA+AGG | + | chr8.2:1671694-1671713 | None:intergenic | 40.0% | |
| AGGAATGTCCATGTAATTGC+AGG | - | chr8.2:1671909-1671928 | MS.gene68949:CDS | 40.0% | |
| CAAGATGAATCACAGTGTTC+AGG | + | chr8.2:1671209-1671228 | None:intergenic | 40.0% | |
| CAATGTCAGGATCAATCCAA+AGG | - | chr8.2:1671464-1671483 | MS.gene68949:intron | 40.0% | |
| CTCATAGCAAAAGAACACTC+TGG | + | chr8.2:1671263-1671282 | None:intergenic | 40.0% | |
| CTTTGAACTCCACATGTTAC+TGG | + | chr8.2:1672142-1672161 | None:intergenic | 40.0% | |
| GAACCTCAGATCTTGCTTAA+GGG | - | chr8.2:1671741-1671760 | MS.gene68949:CDS | 40.0% | |
| GAGTTCAAAGCAATGAGAAC+AGG | - | chr8.2:1672152-1672171 | MS.gene68949:CDS | 40.0% | |
| GCTAAAGAACCAGTAACATG+TGG | - | chr8.2:1672130-1672149 | MS.gene68949:CDS | 40.0% | |
| TGTTCACAAAACACAGGCTT+TGG | + | chr8.2:1671182-1671201 | None:intergenic | 40.0% | |
| TTGGAATTGGAGGTGGTATT+TGG | - | chr8.2:1672089-1672108 | MS.gene68949:CDS | 40.0% | |
| ! | ATGATAATGCAAGGCACAGT+AGG | - | chr8.2:1671889-1671908 | MS.gene68949:CDS | 40.0% |
| ! | GATTACCTTTAGTGGGGTTT+TGG | - | chr8.2:1671686-1671705 | MS.gene68949:CDS | 40.0% |
| ! | GTGCAAAGTATCTATCGACT+TGG | - | chr8.2:1671661-1671680 | MS.gene68949:CDS | 40.0% |
| ! | TGGCAGATCATGATAATGCA+AGG | - | chr8.2:1671880-1671899 | MS.gene68949:CDS | 40.0% |
| !! | GAGTGTTCTTTTGCTATGAG+TGG | - | chr8.2:1671263-1671282 | MS.gene68949:intron | 40.0% |
| !!! | ATTTCAGCTTTTGGAGCACT+TGG | - | chr8.2:1672070-1672089 | MS.gene68949:CDS | 40.0% |
| CGAACCTCAGATCTTGCTTA+AGG | - | chr8.2:1671740-1671759 | MS.gene68949:CDS | 45.0% | |
| GATCCCTTAAGCAAGATCTG+AGG | + | chr8.2:1671747-1671766 | None:intergenic | 45.0% | |
| GGAACTTGATCATAAGGCTG+AGG | + | chr8.2:1671392-1671411 | None:intergenic | 45.0% | |
| TGAGGTTCGAACTTTGTCGA+TGG | + | chr8.2:1671729-1671748 | None:intergenic | 45.0% | |
| TGTCAGGATCAATCCAAAGG+TGG | - | chr8.2:1671467-1671486 | MS.gene68949:intron | 45.0% | |
| TTGATATTCCAGTGACGACG+CGG | - | chr8.2:1671435-1671454 | MS.gene68949:intron | 45.0% | |
| ! | GATTTGGAATTCTGGCTGCA+GGG | - | chr8.2:1672035-1672054 | MS.gene68949:CDS | 45.0% |
| ! | TTTGGAGCACTTGGAATTGG+AGG | - | chr8.2:1672079-1672098 | MS.gene68949:CDS | 45.0% |
| !!! | GCTTTTGGAGCACTTGGAAT+TGG | - | chr8.2:1672076-1672095 | MS.gene68949:CDS | 45.0% |
| ACAAGTTCCACCACCACCTT+TGG | + | chr8.2:1671483-1671502 | None:intergenic | 50.0% | |
| CAGGATCAATCCAAAGGTGG+TGG | - | chr8.2:1671470-1671489 | MS.gene68949:intron | 50.0% | |
| ! | GATCAATCCAAAGGTGGTGG+TGG | - | chr8.2:1671473-1671492 | MS.gene68949:intron | 50.0% |
| ! | GGATTTGGAATTCTGGCTGC+AGG | - | chr8.2:1672034-1672053 | MS.gene68949:CDS | 50.0% |
| !! | AAAGGTGGTGGTGGAACTTG+TGG | - | chr8.2:1671482-1671501 | MS.gene68949:intron | 50.0% |
| GACGCGGTGTCTTCAATGTC+AGG | - | chr8.2:1671451-1671470 | MS.gene68949:intron | 55.0% | |
| ! | GGAGCACTTGGAATTGGAGG+TGG | - | chr8.2:1672082-1672101 | MS.gene68949:CDS | 55.0% |
| TGAAGACACCGCGTCGTCAC+TGG | + | chr8.2:1671446-1671465 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.2 | gene | 1671044 | 1672183 | 1671044 | ID=MS.gene68949 |
| chr8.2 | mRNA | 1671044 | 1672183 | 1671044 | ID=MS.gene68949.t1;Parent=MS.gene68949 |
| chr8.2 | exon | 1671655 | 1672183 | 1671655 | ID=MS.gene68949.t1.exon1;Parent=MS.gene68949.t1 |
| chr8.2 | CDS | 1671655 | 1672183 | 1671655 | ID=cds.MS.gene68949.t1;Parent=MS.gene68949.t1 |
| chr8.2 | exon | 1671297 | 1671341 | 1671297 | ID=MS.gene68949.t1.exon2;Parent=MS.gene68949.t1 |
| chr8.2 | CDS | 1671297 | 1671341 | 1671297 | ID=cds.MS.gene68949.t1;Parent=MS.gene68949.t1 |
| chr8.2 | exon | 1671044 | 1671171 | 1671044 | ID=MS.gene68949.t1.exon3;Parent=MS.gene68949.t1 |
| chr8.2 | CDS | 1671044 | 1671171 | 1671044 | ID=cds.MS.gene68949.t1;Parent=MS.gene68949.t1 |
| Gene Sequence |
| Protein sequence |
