AlfalfaGEDB | MS.gene69008

M. sativa cultivar XinJiangDaYe / MS.gene69008

KEGG: K02357
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003746 GO:0005488 GO:0005515 GO:0006414 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0034645 GO:0043170 GO:0044237 GO:0044249 GO:0044260 GO:0045182 GO:0071704 GO:0090079 GO:0097159 GO:1901363 GO:1901576
PFAM: PF00889.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69008.t1	XP_003620654.1	77.2	250	55	2	15	263	806	1054	2.40E-90	342

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69008.t1	A2BW76	60.5	205	77	4	59	260	4	207	1.5e-61	237.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69008.t1	G7KPU0	77.2	250	55	2	15	263	806	1054	1.7e-90	342.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050026	MS.gene69008	0.832585	8.69E-56	-1.69E-46
MS.gene050759	MS.gene69008	0.820304	7.36E-53	-1.69E-46
MS.gene050962	MS.gene69008	0.811529	6.69E-51	-1.69E-46
MS.gene051279	MS.gene69008	0.809617	1.73E-50	-1.69E-46
MS.gene052582	MS.gene69008	0.818941	1.51E-52	-1.69E-46
MS.gene052584	MS.gene69008	0.817485	3.22E-52	-1.69E-46
MS.gene054291	MS.gene69008	-0.80599	1.02E-49	-1.69E-46
MS.gene054651	MS.gene69008	0.80957	1.77E-50	-1.69E-46
MS.gene056174	MS.gene69008	0.811888	5.59E-51	-1.69E-46
MS.gene059601	MS.gene69008	0.835312	1.81E-56	-1.69E-46
MS.gene059602	MS.gene69008	0.810911	9.11E-51	-1.69E-46
MS.gene060373	MS.gene69008	0.801315	9.54E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69008.t1	MTR_6g088270	88.208	212	23	2	53	263	844	1054	3.82e-121	370
MS.gene69008.t1	MTR_6g088270	55.805	267	112	4	2	263	549	814	9.80e-88	281
MS.gene69008.t1	MTR_2g039780	68.421	171	37	4	94	263	62	216	1.02e-66	206
MS.gene69008.t1	MTR_6g087300	63.587	184	36	3	53	235	204	357	1.42e-63	203
MS.gene69008.t1	MTR_6g087300	40.000	155	46	4	99	253	50	157	8.07e-24	99.0
MS.gene69008.t1	MTR_6g084360	68.072	166	23	1	89	254	973	1108	1.69e-60	206
MS.gene69008.t1	MTR_6g088240	52.406	187	75	5	75	251	1	183	5.01e-49	160
MS.gene69008.t1	MTR_0428s0020	52.308	195	36	4	53	246	38	176	5.26e-48	159
MS.gene69008.t1	MTR_2g039790	76.786	112	11	3	53	163	15	112	8.31e-48	156
MS.gene69008.t1	MTR_6g488290	50.543	184	34	4	51	233	36	163	2.54e-42	143
MS.gene69008.t1	MTR_6g027070	54.305	151	48	4	59	208	13	143	6.32e-41	138
MS.gene69008.t1	MTR_6g088250	67.568	111	30	2	53	162	1255	1360	7.12e-38	141
MS.gene69008.t1	MTR_6g066260	56.790	81	34	1	101	181	18	97	1.03e-23	92.4
MS.gene69008.t1	MTR_6g087200	50.602	83	30	1	118	200	1033	1104	2.78e-19	87.8
MS.gene69008.t1	MTR_6g087190	76.923	39	9	0	106	144	15	53	1.54e-14	67.0
MS.gene69008.t1	MTR_6g003970	42.574	101	56	2	55	154	23	122	8.35e-14	70.5
MS.gene69008.t1	MTR_6g081090	80.000	40	7	1	52	90	4	43	2.17e-11	60.1
MS.gene69008.t1	MTR_6g488300	37.363	91	57	0	26	116	1235	1325	2.90e-11	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene69008.t1	AT4G29060	77.387	199	43	2	56	253	754	951	1.94e-98	308
MS.gene69008.t1	AT4G29060	63.265	245	83	6	22	261	480	722	5.66e-90	286
MS.gene69008.t1	AT4G29060	64.407	236	72	5	22	253	480	707	3.43e-92	286
MS.gene69008.t1	AT4G11120	47.312	93	45	3	62	153	76	165	4.11e-14	71.6

Find 43 sgRNAs with CRISPR-Local

Find 52 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCCTTCGTCACAATGCTTT+CGG	0.198367	6.2:-86929918	None:intergenic
TCGCTGTCTGTCTCAGTTTC+AGG	0.204785	6.2:-86929370	None:intergenic
GCACTCTCATACTTAATAAA+AGG	0.279197	6.2:-86930055	None:intergenic
TCCTTCGTCACAATGCTTTC+GGG	0.281412	6.2:-86929917	None:intergenic
TCCTTCAGCTGCTAGTCTTC+CGG	0.310289	6.2:-86929726	None:intergenic
GCAAGCATATCTCAGGAAAA+AGG	0.318552	6.2:+86929679	MS.gene69008:CDS
CTTGAATTTCTCACTTCTGC+TGG	0.339585	6.2:-86929819	None:intergenic
ATGTCTGATTCTGTAACAAC+AGG	0.394057	6.2:-86929409	None:intergenic
AACAATTAGCTCAATATTCA+AGG	0.400543	6.2:-86929296	None:intergenic
CTCACTTCTGCTGGCAAAGT+CGG	0.427147	6.2:-86929810	None:intergenic
TGCAAAGAGAACACCTTGCT+TGG	0.434411	6.2:+86929954	MS.gene69008:CDS
GGTAGTGGTCTCAGCCTCTT+TGG	0.434988	6.2:+86929574	MS.gene69008:CDS
ATAAGAGAGAAAATTGTTGC+GGG	0.462857	6.2:+86929989	MS.gene69008:CDS
AGTTGCAGCTGCTTGTCCAC+AGG	0.477353	6.2:+86929868	MS.gene69008:CDS
CAGCAGAAGTGAGAAATTCA+AGG	0.486725	6.2:+86929820	MS.gene69008:CDS
GGAAGAATCTCAAAGAGGTT+AGG	0.488046	6.2:+86930010	MS.gene69008:CDS
AACTGAAGGCAATCTTGAAA+AGG	0.490305	6.2:+86929655	MS.gene69008:CDS
AAAAGGCGCAAGCATATCTC+AGG	0.509392	6.2:+86929672	MS.gene69008:CDS
GATTCTGTAACAACAGGGCT+AGG	0.516573	6.2:-86929403	None:intergenic
AAGCAACTACGAGAAGAAAT+CGG	0.523154	6.2:+86929599	MS.gene69008:CDS
TCATCCGCTGATAAGAAATC+CGG	0.529747	6.2:+86929707	MS.gene69008:CDS
CTACGAGAAGAAATCGGTGC+AGG	0.531943	6.2:+86929605	MS.gene69008:CDS
TGTTATCGGGTTTCCAAGCA+AGG	0.532323	6.2:-86929967	None:intergenic
CTAGCAGCTGAAGGAAGAAT+CGG	0.544679	6.2:+86929734	MS.gene69008:CDS
AAGTGAGAAATTCAAGGAGA+TGG	0.546657	6.2:+86929826	MS.gene69008:CDS
TCGTAGTTGCTTGACCAAAG+AGG	0.566443	6.2:-86929588	None:intergenic
ACATTACATCAAGAAACATA+AGG	0.570939	6.2:+86929428	MS.gene69008:CDS
TTGCAGGAAATCAACGGTAG+TGG	0.572178	6.2:+86929559	MS.gene69008:intron
AGGCAAAGTATAGCTGCGAT+TGG	0.573375	6.2:+86930097	MS.gene69008:CDS
CATAAGAGAGAAAATTGTTG+CGG	0.576010	6.2:+86929988	MS.gene69008:CDS
AAAGCTCTTGCTGAAACTGA+AGG	0.578888	6.2:+86929641	MS.gene69008:CDS
AGAAGAAATCGGTGCAGGGA+TGG	0.582771	6.2:+86929610	MS.gene69008:CDS
TCCCGAAAGCATTGTGACGA+AGG	0.594162	6.2:+86929916	MS.gene69008:CDS
TACATTCATGATGCACGCAT+CGG	0.594447	6.2:+86929761	MS.gene69008:CDS
TCCGGAAGACTAGCAGCTGA+AGG	0.605549	6.2:+86929725	MS.gene69008:CDS
TACGAGAAGAAATCGGTGCA+GGG	0.608538	6.2:+86929606	MS.gene69008:CDS
ATAGATACAAACTGAACCTG+TGG	0.617602	6.2:-86929884	None:intergenic
TCTTCCGGATTTCTTATCAG+CGG	0.628603	6.2:-86929711	None:intergenic
TTGCGGGAAGAATCTCAAAG+AGG	0.662649	6.2:+86930005	MS.gene69008:CDS
CTGTAGTAAATGACTTGGTG+AGG	0.674636	6.2:+86930077	MS.gene69008:CDS
TGTCTGATTCTGTAACAACA+GGG	0.689329	6.2:-86929408	None:intergenic
GAGTGCTGTAGTAAATGACT+TGG	0.689561	6.2:+86930072	MS.gene69008:CDS
GTTGTGTTGCAGGAAATCAA+CGG	0.701558	6.2:+86929553	MS.gene69008:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	AATTTTCTCTCTTATGTTAT+CGG	-	chr6.2:86929984-86930003	None:intergenic	20.0%
!	AACAATTAGCTCAATATTCA+AGG	-	chr6.2:86929299-86929318	None:intergenic	25.0%
!	ACATTACATCAAGAAACATA+AGG	+	chr6.2:86929428-86929447	MS.gene69008:CDS	25.0%
!!	ATTTTCTCTCTTATGTTATC+GGG	-	chr6.2:86929983-86930002	None:intergenic	25.0%
	AAACAATCAAAAAGTGACAG+TGG	+	chr6.2:86929331-86929350	MS.gene69008:CDS	30.0%
	ATAAGAGAGAAAATTGTTGC+GGG	+	chr6.2:86929989-86930008	MS.gene69008:CDS	30.0%
	CATAAGAGAGAAAATTGTTG+CGG	+	chr6.2:86929988-86930007	MS.gene69008:CDS	30.0%
	GCACTCTCATACTTAATAAA+AGG	-	chr6.2:86930058-86930077	None:intergenic	30.0%
!	TCGCATTCGTATTTTACATA+TGG	+	chr6.2:86929488-86929507	MS.gene69008:intron	30.0%
	AACTGAAGGCAATCTTGAAA+AGG	+	chr6.2:86929655-86929674	MS.gene69008:CDS	35.0%
	AAGCAACTACGAGAAGAAAT+CGG	+	chr6.2:86929599-86929618	MS.gene69008:CDS	35.0%
	AAGTGAGAAATTCAAGGAGA+TGG	+	chr6.2:86929826-86929845	MS.gene69008:CDS	35.0%
	ATAGATACAAACTGAACCTG+TGG	-	chr6.2:86929887-86929906	None:intergenic	35.0%
	ATCAAAAAGTGACAGTGGAA+AGG	+	chr6.2:86929336-86929355	MS.gene69008:CDS	35.0%
	ATGTCTGATTCTGTAACAAC+AGG	-	chr6.2:86929412-86929431	None:intergenic	35.0%
	CAAGCATATCTCAGGAAAAA+GGG	+	chr6.2:86929680-86929699	MS.gene69008:CDS	35.0%
	TGTCTGATTCTGTAACAACA+GGG	-	chr6.2:86929411-86929430	None:intergenic	35.0%
!!!	TTAAAGTGCTGCGTTTTGTT+AGG	+	chr6.2:86930128-86930147	MS.gene69008:CDS	35.0%
	AAAGCTCTTGCTGAAACTGA+AGG	+	chr6.2:86929641-86929660	MS.gene69008:CDS	40.0%
	CAGCAGAAGTGAGAAATTCA+AGG	+	chr6.2:86929820-86929839	MS.gene69008:CDS	40.0%
	CTTGAATTTCTCACTTCTGC+TGG	-	chr6.2:86929822-86929841	None:intergenic	40.0%
	CTTGTTCAATGTTGTGTTGC+AGG	+	chr6.2:86929543-86929562	MS.gene69008:intron	40.0%
	GCAAGCATATCTCAGGAAAA+AGG	+	chr6.2:86929679-86929698	MS.gene69008:CDS	40.0%
	GGAAGAATCTCAAAGAGGTT+AGG	+	chr6.2:86930010-86930029	MS.gene69008:CDS	40.0%
	GTTGTGTTGCAGGAAATCAA+CGG	+	chr6.2:86929553-86929572	MS.gene69008:intron	40.0%
	TACATTCATGATGCACGCAT+CGG	+	chr6.2:86929761-86929780	MS.gene69008:CDS	40.0%
	TCATCCGCTGATAAGAAATC+CGG	+	chr6.2:86929707-86929726	MS.gene69008:CDS	40.0%
	TCTTCCGGATTTCTTATCAG+CGG	-	chr6.2:86929714-86929733	None:intergenic	40.0%
	TTCCTTCGTCACAATGCTTT+CGG	-	chr6.2:86929921-86929940	None:intergenic	40.0%
!	GAGTGCTGTAGTAAATGACT+TGG	+	chr6.2:86930072-86930091	MS.gene69008:CDS	40.0%
!!	CGTTTTGTTAGGTTCACTCT+TGG	+	chr6.2:86930139-86930158	MS.gene69008:CDS	40.0%
!!	CTGTAGTAAATGACTTGGTG+AGG	+	chr6.2:86930077-86930096	MS.gene69008:CDS	40.0%
	AGGCAAAGTATAGCTGCGAT+TGG	+	chr6.2:86930097-86930116	MS.gene69008:CDS	45.0%
	CTAGCAGCTGAAGGAAGAAT+CGG	+	chr6.2:86929734-86929753	MS.gene69008:CDS	45.0%
	GATTCTGTAACAACAGGGCT+AGG	-	chr6.2:86929406-86929425	None:intergenic	45.0%
	TACGAGAAGAAATCGGTGCA+GGG	+	chr6.2:86929606-86929625	MS.gene69008:CDS	45.0%
	TCCTTCGTCACAATGCTTTC+GGG	-	chr6.2:86929920-86929939	None:intergenic	45.0%
	TCGTAGTTGCTTGACCAAAG+AGG	-	chr6.2:86929591-86929610	None:intergenic	45.0%
	TGCAAAGAGAACACCTTGCT+TGG	+	chr6.2:86929954-86929973	MS.gene69008:CDS	45.0%
	TGTTATCGGGTTTCCAAGCA+AGG	-	chr6.2:86929970-86929989	None:intergenic	45.0%
	TTGCAGGAAATCAACGGTAG+TGG	+	chr6.2:86929559-86929578	MS.gene69008:intron	45.0%
	TTGCGGGAAGAATCTCAAAG+AGG	+	chr6.2:86930005-86930024	MS.gene69008:CDS	45.0%
!	AAAAGGCGCAAGCATATCTC+AGG	+	chr6.2:86929672-86929691	MS.gene69008:CDS	45.0%
	AGAAGAAATCGGTGCAGGGA+TGG	+	chr6.2:86929610-86929629	MS.gene69008:CDS	50.0%
	CTACGAGAAGAAATCGGTGC+AGG	+	chr6.2:86929605-86929624	MS.gene69008:CDS	50.0%
	CTCACTTCTGCTGGCAAAGT+CGG	-	chr6.2:86929813-86929832	None:intergenic	50.0%
	TCGCTGTCTGTCTCAGTTTC+AGG	-	chr6.2:86929373-86929392	None:intergenic	50.0%
!!	TCCCGAAAGCATTGTGACGA+AGG	+	chr6.2:86929916-86929935	MS.gene69008:CDS	50.0%
!!	TCCTTCAGCTGCTAGTCTTC+CGG	-	chr6.2:86929729-86929748	None:intergenic	50.0%
	AGTTGCAGCTGCTTGTCCAC+AGG	+	chr6.2:86929868-86929887	MS.gene69008:CDS	55.0%
	TCCGGAAGACTAGCAGCTGA+AGG	+	chr6.2:86929725-86929744	MS.gene69008:CDS	55.0%
!	GGTAGTGGTCTCAGCCTCTT+TGG	+	chr6.2:86929574-86929593	MS.gene69008:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.2	gene	86929292	86930198	86929292	ID=MS.gene69008
chr6.2	mRNA	86929292	86930198	86929292	ID=MS.gene69008.t1;Parent=MS.gene69008
chr6.2	exon	86929292	86929449	86929292	ID=MS.gene69008.t1.exon1;Parent=MS.gene69008.t1
chr6.2	CDS	86929292	86929449	86929292	ID=cds.MS.gene69008.t1;Parent=MS.gene69008.t1
chr6.2	exon	86929565	86930198	86929565	ID=MS.gene69008.t1.exon2;Parent=MS.gene69008.t1
chr6.2	CDS	86929565	86930198	86929565	ID=cds.MS.gene69008.t1;Parent=MS.gene69008.t1

Gene Sequence

>MS.gene69008

ATGGCCTTGAATATTGAGCTAATTGTTGCAGAAGATAAAAAACAATCAAAAAGTGACAGTGGAAAGGAAGAAGTTGCACCTGAAACTGAGACAGACAGCGATGCATTTGATCCTAGCCCTGTTGTTACAGAATCAGACATTACATCAAGAAACATAAGGTTCTTGTTTTTTATCTCACACTTTTTCTGCTAATTTATCGCATTCGTATTTTACATATGGAAATACTGATTTTATCGCATTCATATCTCACACTTGTTCAATGTTGTGTTGCAGGAAATCAACGGTAGTGGTCTCAGCCTCTTTGGTCAAGCAACTACGAGAAGAAATCGGTGCAGGGATGGACTGTAAGAAAGCTCTTGCTGAAACTGAAGGCAATCTTGAAAAGGCGCAAGCATATCTCAGGAAAAAGGGTCTCTCATCCGCTGATAAGAAATCCGGAAGACTAGCAGCTGAAGGAAGAATCGGTACATACATTCATGATGCACGCATCGGTGTTCTAATTGAAGTGAACTGTGAAACCGACTTTGCCAGCAGAAGTGAGAAATTCAAGGAGATGGTTGATGATCTTGCAATGCAAGTTGCAGCTGCTTGTCCACAGGTTCAGTTTGTATCTATTGAAGATGTTCCCGAAAGCATTGTGACGAAGGAAAAAGAACTTGAGATGCAAAGAGAACACCTTGCTTGGAAACCCGATAACATAAGAGAGAAAATTGTTGCGGGAAGAATCTCAAAGAGGTTAGGAGAGCTTGCTCTTCTAGAGCAGCCTTTTATTAAGTATGAGAGTGCTGTAGTAAATGACTTGGTGAGGCAAAGTATAGCTGCGATTGGCGAGAACTTTAAAGTGCTGCGTTTTGTTAGGTTCACTCTTGGAGAAACAGATAAAAAAGAAACAGACTTGCCAGCATAG

Protein sequence

>MS.gene69008.t1

MALNIELIVAEDKKQSKSDSGKEEVAPETETDSDAFDPSPVVTESDITSRNIRKSTVVVSASLVKQLREEIGAGMDCKKALAETEGNLEKAQAYLRKKGLSSADKKSGRLAAEGRIGTYIHDARIGVLIEVNCETDFASRSEKFKEMVDDLAMQVAAACPQVQFVSIEDVPESIVTKEKELEMQREHLAWKPDNIREKIVAGRISKRLGELALLEQPFIKYESAVVNDLVRQSIAAIGENFKVLRFVRFTLGETDKKETDLPA