AlfalfaGEDB | MS.gene059602

M. sativa cultivar XinJiangDaYe / MS.gene059602

KEGG: K02357
GO: GO:0003674 GO:0003676 GO:0003723 GO:0003746 GO:0005488 GO:0006414 GO:0008135 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009987 GO:0034645 GO:0043170 GO:0044237 GO:0044249 GO:0044260 GO:0045182 GO:0071704 GO:0090079 GO:0097159 GO:1901363 GO:1901576
PFAM: PF00889.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059602.t1	XP_003620654.1	92.1	89	7	0	1	89	945	1033	5.80E-34	153.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059602.t1	A2BW76	66.3	89	30	0	1	89	100	188	1.9e-25	116.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059602.t1	G7KPU0	92.1	89	7	0	1	89	945	1033	4.2e-34	153.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052519	MS.gene059602	0.807747	4.35E-50	-1.69E-46
MS.gene054236	MS.gene059602	0.806763	7.02E-50	-1.69E-46
MS.gene054775	MS.gene059602	0.829096	6.23E-55	-1.69E-46
MS.gene058625	MS.gene059602	0.803486	3.41E-49	-1.69E-46
MS.gene059272	MS.gene059602	0.811799	5.84E-51	-1.69E-46
MS.gene059602	MS.gene061477	0.832006	1.21E-55	-1.69E-46
MS.gene059602	MS.gene061485	0.906092	2.15E-80	-1.69E-46
MS.gene059602	MS.gene062576	0.80364	3.17E-49	-1.69E-46
MS.gene059602	MS.gene064321	0.801987	6.95E-49	-1.69E-46
MS.gene059602	MS.gene066335	0.834335	3.18E-56	-1.69E-46
MS.gene059602	MS.gene066782	0.83343	5.36E-56	-1.69E-46
MS.gene059602	MS.gene066784	0.834085	3.67E-56	-1.69E-46
MS.gene059602	MS.gene073139	0.804133	2.50E-49	-1.69E-46
MS.gene059602	MS.gene07876	0.80433	2.28E-49	-1.69E-46
MS.gene059602	MS.gene09233	0.805349	1.39E-49	-1.69E-46
MS.gene059602	MS.gene21517	0.801449	8.96E-49	-1.69E-46
MS.gene059602	MS.gene22707	0.811339	7.36E-51	-1.69E-46
MS.gene059602	MS.gene26380	0.800437	1.44E-48	-1.69E-46
MS.gene059602	MS.gene26659	0.803749	3.01E-49	-1.69E-46
MS.gene059602	MS.gene27421	0.814423	1.55E-51	-1.69E-46
MS.gene059602	MS.gene31433	0.810532	1.10E-50	-1.69E-46
MS.gene059602	MS.gene48919	0.802778	4.77E-49	-1.69E-46
MS.gene059602	MS.gene53321	0.805899	1.07E-49	-1.69E-46
MS.gene059602	MS.gene55406	0.807013	6.22E-50	-1.69E-46
MS.gene059602	MS.gene60171	0.801057	1.08E-48	-1.69E-46
MS.gene059602	MS.gene67042	0.910532	1.69E-82	-1.69E-46
MS.gene059602	MS.gene68999	0.847123	1.41E-59	-1.69E-46
MS.gene059602	MS.gene69008	0.810911	9.11E-51	-1.69E-46
MS.gene059602	MS.gene69016	0.825005	5.93E-54	-1.69E-46
MS.gene059602	MS.gene71440	0.807752	4.33E-50	-1.69E-46
MS.gene059602	MS.gene72400	0.805964	1.04E-49	-1.69E-46
MS.gene059602	MS.gene80853	0.80922	2.11E-50	-1.69E-46
MS.gene059602	MS.gene83529	0.801181	1.02E-48	-1.69E-46
MS.gene059602	MS.gene90605	0.808789	2.61E-50	-1.69E-46
MS.gene059602	MS.gene93654	0.802482	5.49E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059602.t1	MTR_6g088270	91.111	90	8	0	1	90	945	1034	1.18e-47	163
MS.gene059602.t1	MTR_6g088270	59.091	110	44	1	1	110	704	812	7.60e-36	129
MS.gene059602.t1	MTR_6g088240	88.235	85	10	0	5	89	90	174	2.35e-45	145
MS.gene059602.t1	MTR_2g039780	86.207	87	11	1	1	86	107	193	2.26e-43	141
MS.gene059602.t1	MTR_6g087300	83.750	80	13	0	3	82	278	357	9.78e-37	128
MS.gene059602.t1	MTR_6g084360	88.333	60	7	0	30	89	1037	1096	1.10e-26	103
MS.gene059602.t1	MTR_6g027070	65.455	55	19	0	1	55	89	143	7.55e-16	69.3

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene059602.t1	AT4G29060	75.556	90	22	1	90	852	941	1.08e-37	135
MS.gene059602.t1	AT4G29060	71.111	90	26	1	90	614	703	1.96e-34	125
MS.gene059602.t1	AT4G29060	77.108	83	19	8	90	615	697	8.36e-35	126

Find 23 sgRNAs with CRISPR-Local

Find 26 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGGTCTTCTCTTTGCATTTC+AGG	0.133913	6.4:+60973802	None:intergenic
TTCCTTCGTCACAATAATTT+CGG	0.214145	6.4:+60973831	None:intergenic
TATAGAGCAGCCTTTCATTA+AGG	0.293654	6.4:-60973704	MS.gene059602:CDS
TCCTTCGTCACAATAATTTC+GGG	0.355650	6.4:+60973832	None:intergenic
GCAAGTTGCTGCTTGTCCTC+AGG	0.414009	6.4:-60973881	MS.gene059602:CDS
GGAAGAATCTCAAAGAGGTT+AGG	0.441763	6.4:-60973739	MS.gene059602:CDS
ATAAGAGAGAAAATTGTTGA+GGG	0.490179	6.4:-60973760	MS.gene059602:CDS
GCATTTAATGTTCTCTCCAA+TGG	0.498936	6.4:+60973636	None:intergenic
TCAAATGAGCTGTGCTGCTG+TGG	0.512981	6.4:+60973574	None:intergenic
CATAAGAGAGAAAATTGTTG+AGG	0.519674	6.4:-60973761	MS.gene059602:CDS
TGTTCTCGGGTTTCAAAGCA+AGG	0.540905	6.4:+60973782	None:intergenic
ATATCTTCAATAGATACAAA+CGG	0.551658	6.4:+60973856	None:intergenic
AGGCAAAGTATAGCTGCCAT+TGG	0.552004	6.4:-60973652	MS.gene059602:CDS
GCATAAGGCGAACTATATGT+TGG	0.559489	6.4:-60973546	MS.gene059602:CDS
TCCCGAAATTATTGTGACGA+AGG	0.563159	6.4:-60973833	MS.gene059602:CDS
GCTCATTTGACAGAAGCATA+AGG	0.569196	6.4:-60973561	MS.gene059602:CDS
ACACTGTCATCCTTAATGAA+AGG	0.591158	6.4:+60973694	None:intergenic
TTTCAGTGAAAGACTTGGTG+AGG	0.596572	6.4:-60973672	MS.gene059602:CDS
TTGAGGGAAGAATCTCAAAG+AGG	0.636699	6.4:-60973744	MS.gene059602:CDS
CAGTGTTTCAGTGAAAGACT+TGG	0.652429	6.4:-60973677	MS.gene059602:CDS
ATAGATACAAACGGAACCTG+AGG	0.668908	6.4:+60973865	None:intergenic
GGTTCACTCTCTGTAAAACA+TGG	0.671192	6.4:-60973601	MS.gene059602:CDS
TCACTCTCTGTAAAACATGG+TGG	0.691839	6.4:-60973598	MS.gene059602:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATATCTTCAATAGATACAAA+CGG	+	chr6.4:60973555-60973574	None:intergenic	20.0%
!	ATAAGAGAGAAAATTGTTGA+GGG	-	chr6.4:60973648-60973667	MS.gene059602:CDS	25.0%
!!	AATTTTCTCTCTTATGTTCT+CGG	+	chr6.4:60973643-60973662	None:intergenic	25.0%
	CATAAGAGAGAAAATTGTTG+AGG	-	chr6.4:60973647-60973666	MS.gene059602:CDS	30.0%
	TTCCTTCGTCACAATAATTT+CGG	+	chr6.4:60973580-60973599	None:intergenic	30.0%
!	ATTTTCTCTCTTATGTTCTC+GGG	+	chr6.4:60973642-60973661	None:intergenic	30.0%
!!	ATTAAATGCGTCGTTTTGTT+CGG	-	chr6.4:60973786-60973805	MS.gene059602:CDS	30.0%
	ACACTGTCATCCTTAATGAA+AGG	+	chr6.4:60973717-60973736	None:intergenic	35.0%
	GCATTTAATGTTCTCTCCAA+TGG	+	chr6.4:60973775-60973794	None:intergenic	35.0%
	TATAGAGCAGCCTTTCATTA+AGG	-	chr6.4:60973704-60973723	MS.gene059602:CDS	35.0%
	TCCTTCGTCACAATAATTTC+GGG	+	chr6.4:60973579-60973598	None:intergenic	35.0%
	AGGTCTTCTCTTTGCATTTC+AGG	+	chr6.4:60973609-60973628	None:intergenic	40.0%
	ATAGATACAAACGGAACCTG+AGG	+	chr6.4:60973546-60973565	None:intergenic	40.0%
	CAGTGTTTCAGTGAAAGACT+TGG	-	chr6.4:60973731-60973750	MS.gene059602:CDS	40.0%
	GCATAAGGCGAACTATATGT+TGG	-	chr6.4:60973862-60973881	MS.gene059602:CDS	40.0%
	GGAAGAATCTCAAAGAGGTT+AGG	-	chr6.4:60973669-60973688	MS.gene059602:CDS	40.0%
	GGTTCACTCTCTGTAAAACA+TGG	-	chr6.4:60973807-60973826	MS.gene059602:CDS	40.0%
	TCACTCTCTGTAAAACATGG+TGG	-	chr6.4:60973810-60973829	MS.gene059602:CDS	40.0%
	TCCCGAAATTATTGTGACGA+AGG	-	chr6.4:60973575-60973594	MS.gene059602:CDS	40.0%
	TTGAGGGAAGAATCTCAAAG+AGG	-	chr6.4:60973664-60973683	MS.gene059602:CDS	40.0%
!	GCTCATTTGACAGAAGCATA+AGG	-	chr6.4:60973847-60973866	MS.gene059602:CDS	40.0%
!!	TTTCAGTGAAAGACTTGGTG+AGG	-	chr6.4:60973736-60973755	MS.gene059602:CDS	40.0%
	AGGCAAAGTATAGCTGCCAT+TGG	-	chr6.4:60973756-60973775	MS.gene059602:CDS	45.0%
	TGTTCTCGGGTTTCAAAGCA+AGG	+	chr6.4:60973629-60973648	None:intergenic	45.0%
!	TCAAATGAGCTGTGCTGCTG+TGG	+	chr6.4:60973837-60973856	None:intergenic	50.0%
!	GCAAGTTGCTGCTTGTCCTC+AGG	-	chr6.4:60973527-60973546	MS.gene059602:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.4	gene	60973525	60973905	60973525	ID=MS.gene059602
chr6.4	mRNA	60973525	60973905	60973525	ID=MS.gene059602.t1;Parent=MS.gene059602
chr6.4	exon	60973525	60973905	60973525	ID=MS.gene059602.t1.exon1;Parent=MS.gene059602.t1
chr6.4	CDS	60973525	60973905	60973525	ID=cds.MS.gene059602.t1;Parent=MS.gene059602.t1

Gene Sequence

>MS.gene059602

ATGCAAGTTGCTGCTTGTCCTCAGGTTCCGTTTGTATCTATTGAAGATATTCCCGAAATTATTGTGACGAAGGAAAAAGAACCTGAAATGCAAAGAGAAGACCTTGCTTTGAAACCCGAGAACATAAGAGAGAAAATTGTTGAGGGAAGAATCTCAAAGAGGTTAGGAGATCTTGCTCTTATAGAGCAGCCTTTCATTAAGGATGACAGTGTTTCAGTGAAAGACTTGGTGAGGCAAAGTATAGCTGCCATTGGAGAGAACATTAAATGCGTCGTTTTGTTCGGTTCACTCTCTGTAAAACATGGTGGACCACAGCAGCACAGCTCATTTGACAGAAGCATAAGGCGAACTATATGTTGGTTTTGTTCTATGTCAAGTTAA

Protein sequence

>MS.gene059602.t1

MQVAACPQVPFVSIEDIPEIIVTKEKEPEMQREDLALKPENIREKIVEGRISKRLGDLALIEQPFIKDDSVSVKDLVRQSIAAIGENIKCVVLFGSLSVKHGGPQQHSSFDRSIRRTICWFCSMSS