Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69619.t1 | XP_024639677.1 | 81.2 | 442 | 72 | 5 | 1 | 439 | 1 | 434 | 2.30E-192 | 681.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69619.t1 | A0A396HN25 | 80.2 | 308 | 57 | 2 | 1 | 307 | 1 | 305 | 4.5e-134 | 487.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052253 | MS.gene69619 | 0.819774 | 9.73E-53 | -1.69E-46 |
| MS.gene053873 | MS.gene69619 | 0.812875 | 3.40E-51 | -1.69E-46 |
| MS.gene054011 | MS.gene69619 | 0.809202 | 2.13E-50 | -1.69E-46 |
| MS.gene054183 | MS.gene69619 | 0.830431 | 2.95E-55 | -1.69E-46 |
| MS.gene056514 | MS.gene69619 | 0.829524 | 4.91E-55 | -1.69E-46 |
| MS.gene05702 | MS.gene69619 | 0.812378 | 4.37E-51 | -1.69E-46 |
| MS.gene057888 | MS.gene69619 | 0.81635 | 5.79E-52 | -1.69E-46 |
| MS.gene05886 | MS.gene69619 | 0.82151 | 3.89E-53 | -1.69E-46 |
| MS.gene059420 | MS.gene69619 | 0.818114 | 2.32E-52 | -1.69E-46 |
| MS.gene059462 | MS.gene69619 | 0.801131 | 1.04E-48 | -1.69E-46 |
| MS.gene061049 | MS.gene69619 | 0.811759 | 5.96E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69619.t1 | MTR_5g028510 | 59.683 | 315 | 97 | 4 | 74 | 362 | 18 | 328 | 2.71e-116 | 345 |
| MS.gene69619.t1 | MTR_5g028930 | 48.137 | 322 | 141 | 10 | 94 | 395 | 2 | 317 | 7.89e-74 | 244 |
| MS.gene69619.t1 | MTR_5g028190 | 51.562 | 192 | 88 | 3 | 174 | 360 | 5 | 196 | 6.22e-51 | 181 |
| MS.gene69619.t1 | MTR_3g058550 | 31.746 | 189 | 108 | 6 | 186 | 360 | 9 | 190 | 9.66e-13 | 67.8 |
| MS.gene69619.t1 | MTR_5g028830 | 55.128 | 78 | 27 | 3 | 334 | 404 | 44 | 120 | 1.72e-12 | 68.2 |
| MS.gene69619.t1 | MTR_2g017930 | 28.934 | 197 | 121 | 7 | 186 | 370 | 37 | 226 | 2.48e-11 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69619.t1 | AT3G18670 | 32.487 | 197 | 116 | 6 | 179 | 367 | 79 | 266 | 8.88e-23 | 102 |
| MS.gene69619.t1 | AT3G18670 | 32.597 | 181 | 105 | 6 | 179 | 351 | 79 | 250 | 7.11e-19 | 90.1 |
| MS.gene69619.t1 | AT5G04690 | 30.270 | 185 | 118 | 6 | 186 | 367 | 111 | 287 | 2.03e-15 | 79.3 |
| MS.gene69619.t1 | AT5G04690 | 31.515 | 165 | 102 | 6 | 186 | 347 | 111 | 267 | 1.93e-12 | 70.1 |
| MS.gene69619.t1 | AT5G04680 | 28.289 | 152 | 101 | 3 | 215 | 366 | 12 | 155 | 5.59e-12 | 68.2 |
| MS.gene69619.t1 | AT5G04680 | 28.289 | 152 | 101 | 3 | 215 | 366 | 135 | 278 | 6.22e-12 | 68.2 |
| MS.gene69619.t1 | AT5G04680 | 28.289 | 152 | 101 | 3 | 215 | 366 | 135 | 278 | 6.67e-12 | 68.2 |
| MS.gene69619.t1 | AT5G04700 | 27.717 | 184 | 122 | 5 | 186 | 366 | 116 | 291 | 7.48e-12 | 67.8 |
| MS.gene69619.t1 | AT5G04700 | 27.717 | 184 | 122 | 5 | 186 | 366 | 116 | 291 | 9.69e-12 | 67.8 |
| MS.gene69619.t1 | AT5G04690 | 28.723 | 188 | 103 | 8 | 186 | 367 | 111 | 273 | 1.65e-11 | 67.0 |
Find 98 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTAAATTACAATAGTAAA+TGG | 0.128853 | 5.3:+17723871 | MS.gene69619:CDS |
| CCCATTTACTATTGTAATTT+AGG | 0.128882 | 5.3:-17723872 | None:intergenic |
| ACTATTGTAATTTAGGAAAA+TGG | 0.168982 | 5.3:-17723865 | None:intergenic |
| AACATACCCAATAGCCTTAA+AGG | 0.222919 | 5.3:-17723310 | None:intergenic |
| GTTGCTGCCTTTAAGGCTAT+TGG | 0.223500 | 5.3:+17723303 | MS.gene69619:CDS |
| AATTGTTGGTTGTAGTTATA+AGG | 0.249333 | 5.3:-17723911 | None:intergenic |
| CTTTGCCATATCAATGTTTC+CGG | 0.257336 | 5.3:-17698302 | None:intergenic |
| TGCATGGTAGAGAGGGAAAA+TGG | 0.264948 | 5.3:+17698325 | MS.gene69619:CDS |
| CTCGCAATGTTCACAATATA+AGG | 0.267362 | 5.3:-17723193 | None:intergenic |
| TTCTGGTATCTAGATATCTT+TGG | 0.293147 | 5.3:+17723453 | MS.gene69619:CDS |
| TTATTGAAATGCAGCTGATT+TGG | 0.297060 | 5.3:+17698033 | MS.gene69619:intron |
| TTGCTGCCTTTAAGGCTATT+GGG | 0.313247 | 5.3:+17723304 | MS.gene69619:CDS |
| TTTCCAAGTGGCAGCCGATT+TGG | 0.319200 | 5.3:+17705890 | MS.gene69619:CDS |
| GGTCTTCATGAGACTTCTTC+CGG | 0.328404 | 5.3:+17723232 | MS.gene69619:CDS |
| TACGAACCTTGCTCAATATC+TGG | 0.332391 | 5.3:-17697469 | None:intergenic |
| TTAGCAGCAGCTATAAGAAT+TGG | 0.340840 | 5.3:-17698394 | None:intergenic |
| ATCATGTGGAAAACGTATTC+AGG | 0.347448 | 5.3:+17697676 | MS.gene69619:CDS |
| TTGTCAGAGGTGAGTACTTC+AGG | 0.349827 | 5.3:-17697301 | None:intergenic |
| AAGTTGAAAGAGAAGTTTCT+TGG | 0.355395 | 5.3:+17697615 | MS.gene69619:CDS |
| ATACACGGCAGAACTCAAAA+AGG | 0.356094 | 5.3:+17697752 | MS.gene69619:CDS |
| GTCGAGAGAGGTGTCTATAT+TGG | 0.381319 | 5.3:+17698097 | MS.gene69619:CDS |
| TACAGATTTAAGTGTAGAAA+AGG | 0.415708 | 5.3:+17723147 | MS.gene69619:intron |
| TTGTACCGGAAACATTGATA+TGG | 0.420604 | 5.3:+17698297 | MS.gene69619:CDS |
| CCTAAATTACAATAGTAAAT+GGG | 0.423080 | 5.3:+17723872 | MS.gene69619:CDS |
| AAAGGTTTGTGGGACTTTGT+TGG | 0.423263 | 5.3:+17697367 | MS.gene69619:CDS |
| TGTATCACAGCTGAAATATT+CGG | 0.431976 | 5.3:+17698514 | MS.gene69619:CDS |
| GTCTTCATGAGACTTCTTCC+GGG | 0.435577 | 5.3:+17723233 | MS.gene69619:CDS |
| GCTCTTGTTGCTCGTTGTAC+CGG | 0.441811 | 5.3:+17698283 | MS.gene69619:CDS |
| AACATTGCGAGGCGGGCTAC+AGG | 0.442779 | 5.3:+17723205 | MS.gene69619:CDS |
| TGTTGGTGAGGCTTCTTCAC+TGG | 0.446866 | 5.3:+17697384 | MS.gene69619:CDS |
| ATCTGATGGGGAAAGGTTTG+TGG | 0.448881 | 5.3:+17697356 | MS.gene69619:CDS |
| CTTCACGTTGCAGTAGTTGC+TGG | 0.451839 | 5.3:+17698175 | MS.gene69619:CDS |
| TAGCAGCAGCTATAAGAATT+GGG | 0.454519 | 5.3:-17698393 | None:intergenic |
| AAATAGTGTGTTAGTTGCAT+TGG | 0.462809 | 5.3:+17705850 | MS.gene69619:CDS |
| TATTGTGAACATTGCGAGGC+GGG | 0.463067 | 5.3:+17723198 | MS.gene69619:CDS |
| ACATACTGACCAACATTTCC+CGG | 0.463530 | 5.3:-17723251 | None:intergenic |
| CTCCTGAATATATGCTTATG+TGG | 0.463666 | 5.3:-17698073 | None:intergenic |
| AACCACATAAGCATATATTC+AGG | 0.470862 | 5.3:+17698071 | MS.gene69619:CDS |
| GCGAGGCGGGCTACAGGCAG+TGG | 0.471951 | 5.3:+17723211 | MS.gene69619:CDS |
| TTTAGGAAAATGGTGTGTAA+TGG | 0.472673 | 5.3:-17723855 | None:intergenic |
| AAGTGTAGAAAAGGAATTTG+CGG | 0.472694 | 5.3:+17723156 | MS.gene69619:CDS |
| TCTGATGGGGAAAGGTTTGT+GGG | 0.474381 | 5.3:+17697357 | MS.gene69619:CDS |
| TTTCAACTTCAGAAACACTT+TGG | 0.479499 | 5.3:-17697601 | None:intergenic |
| AGGGAAACTGTATCCATTGA+AGG | 0.481361 | 5.3:+17697772 | MS.gene69619:CDS |
| ACGTACTTACTGTCCTTCAA+TGG | 0.486233 | 5.3:-17697785 | None:intergenic |
| CAGGTCGACTAATTGCATAC+TGG | 0.488485 | 5.3:+17723418 | MS.gene69619:intron |
| AGTATGTCGCTTCTGCAGAC+TGG | 0.488544 | 5.3:+17723267 | MS.gene69619:CDS |
| ATTGGATCCGTCGAACAGTC+TGG | 0.491750 | 5.3:-17698461 | None:intergenic |
| ATTGTTCTCGAAGTCCAAAT+CGG | 0.492390 | 5.3:-17705904 | None:intergenic |
| GCCACGAGTGTTGCCTCGGT+TGG | 0.497485 | 5.3:+17697271 | MS.gene69619:CDS |
| TTTATGACAAATTATCTGAT+GGG | 0.503147 | 5.3:+17697343 | MS.gene69619:CDS |
| AGTCCAAATCGGCTGCCACT+TGG | 0.504076 | 5.3:-17705893 | None:intergenic |
| ATATATATAAAATCCGCTTC+TGG | 0.516537 | 5.3:+17698142 | MS.gene69619:CDS |
| TTTGTATAGACGTAGCTTTG+TGG | 0.520847 | 5.3:+17705768 | MS.gene69619:intron |
| ACAAATTATCTGATGGGGAA+AGG | 0.521028 | 5.3:+17697349 | MS.gene69619:CDS |
| TGTGGAAAATGTGGAAATGT+TGG | 0.528036 | 5.3:+17698201 | MS.gene69619:CDS |
| TGCTGGAAATGTGGAAAATG+TGG | 0.529311 | 5.3:+17698192 | MS.gene69619:CDS |
| CCTCTGACAACTATGAAAGA+TGG | 0.529550 | 5.3:+17697314 | MS.gene69619:CDS |
| GCATGGTAGAGAGGGAAAAT+GGG | 0.531322 | 5.3:+17698326 | MS.gene69619:CDS |
| GGGAAAATGGGTCTCATGAA+AGG | 0.537848 | 5.3:+17698338 | MS.gene69619:CDS |
| AGACTTCTTCCGGGAAATGT+TGG | 0.537975 | 5.3:+17723242 | MS.gene69619:CDS |
| TCCGTCGAACAGTCTGGTGG+GGG | 0.543506 | 5.3:-17698455 | None:intergenic |
| ATCCGTCGAACAGTCTGGTG+GGG | 0.543713 | 5.3:-17698456 | None:intergenic |
| GGCAAAGTGCATGGTAGAGA+GGG | 0.545786 | 5.3:+17698318 | MS.gene69619:CDS |
| CAATAAACAGATCTTTGTAC+CGG | 0.548063 | 5.3:-17705799 | None:intergenic |
| TTATGACAAATTATCTGATG+GGG | 0.553801 | 5.3:+17697344 | MS.gene69619:CDS |
| GATCCGTCGAACAGTCTGGT+GGG | 0.557916 | 5.3:-17698457 | None:intergenic |
| CATTGATATGGCAAAGTGCA+TGG | 0.563736 | 5.3:+17698309 | MS.gene69619:CDS |
| GCTGATTTGGACGATAGCCT+TGG | 0.565463 | 5.3:+17698046 | MS.gene69619:intron |
| TTAAAACAGCTAGCGAAGCA+TGG | 0.566778 | 5.3:+17697714 | MS.gene69619:CDS |
| GAAAGCCACGAGTGTTGCCT+CGG | 0.571659 | 5.3:+17697267 | MS.gene69619:CDS |
| CATATCATTCAAATATCATG+TGG | 0.575728 | 5.3:+17697662 | MS.gene69619:CDS |
| TTCCTCAAGGTCTCTGAACG+AGG | 0.576794 | 5.3:-17705825 | None:intergenic |
| TTTGTGGGACTTTGTTGGTG+AGG | 0.584144 | 5.3:+17697372 | MS.gene69619:CDS |
| CTTATATTGTGAACATTGCG+AGG | 0.585173 | 5.3:+17723194 | MS.gene69619:CDS |
| ACACACTATTTGATTCCTCA+AGG | 0.592205 | 5.3:-17705838 | None:intergenic |
| AAGTAGCGTCCTACCAGAAG+CGG | 0.593606 | 5.3:-17698155 | None:intergenic |
| TCGACTAATTGCATACTGGT+TGG | 0.596362 | 5.3:+17723422 | MS.gene69619:CDS |
| ATATAAAATCCGCTTCTGGT+AGG | 0.601973 | 5.3:+17698146 | MS.gene69619:CDS |
| TGCAGTAGTTGCTGGAAATG+TGG | 0.602403 | 5.3:+17698183 | MS.gene69619:CDS |
| CCATCTTTCATAGTTGTCAG+AGG | 0.603453 | 5.3:-17697314 | None:intergenic |
| TGGCAAAGTGCATGGTAGAG+AGG | 0.605185 | 5.3:+17698317 | MS.gene69619:CDS |
| AGCCTCGTTCAGAGACCTTG+AGG | 0.605199 | 5.3:+17705823 | MS.gene69619:CDS |
| ACCTTGCTCAATATCTGGAG+TGG | 0.605299 | 5.3:-17697464 | None:intergenic |
| TAGCGAAGCATGGAATCACT+TGG | 0.606123 | 5.3:+17697724 | MS.gene69619:CDS |
| CACATAAGCATATATTCAGG+AGG | 0.606421 | 5.3:+17698074 | MS.gene69619:CDS |
| TAGCTTTGTGGTTACTCCGC+CGG | 0.608955 | 5.3:+17705780 | MS.gene69619:CDS |
| TCCACTCCAGATATTGAGCA+AGG | 0.611860 | 5.3:+17697463 | MS.gene69619:CDS |
| GGATCCGTCGAACAGTCTGG+TGG | 0.615081 | 5.3:-17698458 | None:intergenic |
| AATCACTTGGCTAACATACA+CGG | 0.615201 | 5.3:+17697737 | MS.gene69619:CDS |
| TATGCTTATGTGGTTCGCCA+AGG | 0.616261 | 5.3:-17698063 | None:intergenic |
| TCCAACCGAGGCAACACTCG+TGG | 0.637413 | 5.3:-17697272 | None:intergenic |
| TAAACAGATCTTTGTACCGG+CGG | 0.655358 | 5.3:-17705796 | None:intergenic |
| GTAAACATGCAAGACAATCA+CGG | 0.656052 | 5.3:+17698247 | MS.gene69619:CDS |
| ATATTGTGAACATTGCGAGG+CGG | 0.669136 | 5.3:+17723197 | MS.gene69619:CDS |
| ATATTCAGGAGGGTCGAGAG+AGG | 0.670243 | 5.3:+17698085 | MS.gene69619:CDS |
| ACATAAGCATATATTCAGGA+GGG | 0.674282 | 5.3:+17698075 | MS.gene69619:CDS |
| TTCAGGAACTATTCCAACCG+AGG | 0.695169 | 5.3:-17697284 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.3 | gene | 17697265 | 17723935 | 17697265 | ID=MS.gene69619 |
| chr5.3 | mRNA | 17697265 | 17723935 | 17697265 | ID=MS.gene69619.t1;Parent=MS.gene69619 |
| chr5.3 | exon | 17697265 | 17697484 | 17697265 | ID=MS.gene69619.t1.exon1;Parent=MS.gene69619.t1 |
| chr5.3 | CDS | 17697265 | 17697484 | 17697265 | ID=cds.MS.gene69619.t1;Parent=MS.gene69619.t1 |
| chr5.3 | exon | 17697585 | 17697797 | 17697585 | ID=MS.gene69619.t1.exon2;Parent=MS.gene69619.t1 |
| chr5.3 | CDS | 17697585 | 17697797 | 17697585 | ID=cds.MS.gene69619.t1;Parent=MS.gene69619.t1 |
| chr5.3 | exon | 17698047 | 17698535 | 17698047 | ID=MS.gene69619.t1.exon3;Parent=MS.gene69619.t1 |
| chr5.3 | CDS | 17698047 | 17698535 | 17698047 | ID=cds.MS.gene69619.t1;Parent=MS.gene69619.t1 |
| chr5.3 | exon | 17705777 | 17705938 | 17705777 | ID=MS.gene69619.t1.exon4;Parent=MS.gene69619.t1 |
| chr5.3 | CDS | 17705777 | 17705938 | 17705777 | ID=cds.MS.gene69619.t1;Parent=MS.gene69619.t1 |
| chr5.3 | exon | 17723152 | 17723325 | 17723152 | ID=MS.gene69619.t1.exon5;Parent=MS.gene69619.t1 |
| chr5.3 | CDS | 17723152 | 17723325 | 17723152 | ID=cds.MS.gene69619.t1;Parent=MS.gene69619.t1 |
| chr5.3 | exon | 17723421 | 17723474 | 17723421 | ID=MS.gene69619.t1.exon6;Parent=MS.gene69619.t1 |
| chr5.3 | CDS | 17723421 | 17723474 | 17723421 | ID=cds.MS.gene69619.t1;Parent=MS.gene69619.t1 |
| chr5.3 | exon | 17723838 | 17723935 | 17723838 | ID=MS.gene69619.t1.exon7;Parent=MS.gene69619.t1 |
| chr5.3 | CDS | 17723838 | 17723935 | 17723838 | ID=cds.MS.gene69619.t1;Parent=MS.gene69619.t1 |
| Gene Sequence |
| Protein sequence |
