Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69958.t1 | KEH35118.1 | 83.2 | 667 | 62 | 2 | 3 | 619 | 777 | 1443 | 1.80E-306 | 1061.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69958.t1 | Q9SZ66 | 43.2 | 118 | 61 | 4 | 1 | 116 | 824 | 937 | 1.6e-08 | 62.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69958.t1 | A0A072V165 | 83.2 | 667 | 62 | 2 | 3 | 619 | 777 | 1443 | 1.3e-306 | 1061.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049993 | MS.gene69958 | 0.864837 | 9.12E-65 | -1.69E-46 |
| MS.gene050168 | MS.gene69958 | 0.802429 | 5.63E-49 | -1.69E-46 |
| MS.gene051860 | MS.gene69958 | 0.802704 | 4.95E-49 | -1.69E-46 |
| MS.gene051983 | MS.gene69958 | 0.810979 | 8.80E-51 | -1.69E-46 |
| MS.gene052011 | MS.gene69958 | -0.826183 | 3.12E-54 | -1.69E-46 |
| MS.gene054183 | MS.gene69958 | 0.856176 | 3.84E-62 | -1.69E-46 |
| MS.gene055358 | MS.gene69958 | 0.838244 | 3.23E-57 | -1.69E-46 |
| MS.gene055604 | MS.gene69958 | 0.801113 | 1.05E-48 | -1.69E-46 |
| MS.gene055701 | MS.gene69958 | 0.817941 | 2.54E-52 | -1.69E-46 |
| MS.gene056723 | MS.gene69958 | 0.804859 | 1.76E-49 | -1.69E-46 |
| MS.gene056979 | MS.gene69958 | 0.801585 | 8.40E-49 | -1.69E-46 |
| MS.gene059609 | MS.gene69958 | 0.803047 | 4.20E-49 | -1.69E-46 |
| MS.gene061114 | MS.gene69958 | -0.844831 | 5.93E-59 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69958.t1 | MTR_3g479510 | 82.960 | 669 | 64 | 2 | 1 | 619 | 775 | 1443 | 0.0 | 1085 |
| MS.gene69958.t1 | MTR_3g058880 | 34.091 | 352 | 157 | 14 | 2 | 326 | 779 | 1082 | 1.76e-34 | 140 |
| MS.gene69958.t1 | MTR_3g058880 | 34.091 | 352 | 157 | 14 | 2 | 326 | 779 | 1082 | 1.77e-34 | 140 |
| MS.gene69958.t1 | MTR_3g058670 | 36.610 | 295 | 129 | 12 | 2 | 284 | 702 | 950 | 8.34e-34 | 138 |
| MS.gene69958.t1 | MTR_3g058870 | 32.615 | 371 | 165 | 16 | 2 | 340 | 780 | 1097 | 1.68e-32 | 134 |
| MS.gene69958.t1 | MTR_3g058840 | 34.000 | 300 | 131 | 12 | 2 | 284 | 275 | 524 | 3.81e-32 | 132 |
| MS.gene69958.t1 | MTR_3g058840 | 34.000 | 300 | 131 | 12 | 2 | 284 | 576 | 825 | 3.88e-32 | 133 |
| MS.gene69958.t1 | MTR_3g058840 | 34.000 | 300 | 131 | 12 | 2 | 284 | 356 | 605 | 4.26e-32 | 132 |
| MS.gene69958.t1 | MTR_3g058710 | 34.247 | 292 | 129 | 12 | 5 | 281 | 50 | 293 | 2.43e-31 | 125 |
| MS.gene69958.t1 | MTR_4g036080 | 33.058 | 363 | 166 | 16 | 2 | 334 | 788 | 1103 | 1.19e-30 | 129 |
| MS.gene69958.t1 | MTR_3g058740 | 35.172 | 290 | 128 | 12 | 2 | 280 | 741 | 981 | 1.74e-28 | 122 |
| MS.gene69958.t1 | MTR_3g058740 | 35.172 | 290 | 128 | 12 | 2 | 280 | 781 | 1021 | 2.12e-28 | 121 |
| MS.gene69958.t1 | MTR_3g058740 | 35.172 | 290 | 128 | 12 | 2 | 280 | 574 | 814 | 3.42e-28 | 120 |
| MS.gene69958.t1 | MTR_3g058740 | 35.172 | 290 | 128 | 12 | 2 | 280 | 545 | 785 | 3.55e-28 | 120 |
| MS.gene69958.t1 | MTR_3g079780 | 30.599 | 317 | 160 | 13 | 11 | 310 | 797 | 1070 | 1.57e-26 | 115 |
| MS.gene69958.t1 | MTR_8g018130 | 30.357 | 336 | 161 | 11 | 3 | 281 | 762 | 1081 | 9.77e-21 | 97.8 |
| MS.gene69958.t1 | MTR_3g079790 | 30.033 | 303 | 134 | 10 | 9 | 284 | 848 | 1099 | 1.21e-20 | 97.4 |
| MS.gene69958.t1 | MTR_2g099920 | 28.824 | 340 | 171 | 12 | 3 | 284 | 767 | 1093 | 4.05e-20 | 95.9 |
| MS.gene69958.t1 | MTR_2g099920 | 28.824 | 340 | 171 | 12 | 3 | 284 | 699 | 1025 | 4.40e-20 | 95.5 |
| MS.gene69958.t1 | MTR_2g099920 | 28.824 | 340 | 171 | 12 | 3 | 284 | 588 | 914 | 4.97e-20 | 95.1 |
| MS.gene69958.t1 | MTR_0005s0110 | 31.085 | 341 | 167 | 13 | 2 | 281 | 766 | 1099 | 7.51e-19 | 91.7 |
| MS.gene69958.t1 | MTR_4g043630 | 31.085 | 341 | 167 | 13 | 2 | 281 | 766 | 1099 | 1.10e-18 | 91.3 |
| MS.gene69958.t1 | MTR_4g043630 | 31.085 | 341 | 167 | 13 | 2 | 281 | 766 | 1099 | 1.13e-18 | 91.3 |
| MS.gene69958.t1 | MTR_4g043630 | 29.545 | 396 | 191 | 15 | 2 | 316 | 766 | 1154 | 1.17e-18 | 91.3 |
| MS.gene69958.t1 | MTR_2g037140 | 29.538 | 325 | 175 | 13 | 2 | 316 | 873 | 1153 | 1.47e-18 | 90.9 |
| MS.gene69958.t1 | MTR_2g037690 | 29.568 | 301 | 150 | 11 | 2 | 284 | 776 | 1032 | 1.97e-18 | 90.5 |
| MS.gene69958.t1 | MTR_3g058780 | 30.034 | 293 | 134 | 10 | 2 | 281 | 766 | 1000 | 6.21e-18 | 88.6 |
| MS.gene69958.t1 | MTR_8g017800 | 27.941 | 340 | 152 | 12 | 2 | 284 | 762 | 1065 | 2.39e-17 | 87.0 |
| MS.gene69958.t1 | MTR_8g017800 | 27.941 | 340 | 152 | 12 | 2 | 284 | 1510 | 1813 | 2.39e-17 | 87.0 |
| MS.gene69958.t1 | MTR_8g018060 | 27.114 | 343 | 165 | 14 | 3 | 284 | 752 | 1070 | 2.93e-16 | 83.2 |
| MS.gene69958.t1 | MTR_5g086690 | 27.622 | 286 | 130 | 11 | 2 | 226 | 758 | 1027 | 2.69e-15 | 80.5 |
| MS.gene69958.t1 | MTR_5g086690 | 29.461 | 241 | 157 | 4 | 387 | 619 | 1248 | 1483 | 1.23e-13 | 75.1 |
| MS.gene69958.t1 | MTR_3g062090 | 26.557 | 305 | 197 | 5 | 312 | 614 | 1658 | 1937 | 3.37e-15 | 80.1 |
| MS.gene69958.t1 | MTR_8g018280 | 29.555 | 247 | 154 | 7 | 382 | 617 | 1230 | 1467 | 1.89e-14 | 77.8 |
| MS.gene69958.t1 | MTR_8g018280 | 28.520 | 277 | 139 | 10 | 2 | 226 | 743 | 1012 | 4.81e-13 | 73.2 |
| MS.gene69958.t1 | MTR_8g018270 | 26.506 | 249 | 167 | 5 | 378 | 617 | 987 | 1228 | 5.08e-13 | 72.8 |
| MS.gene69958.t1 | MTR_8g018170 | 26.266 | 316 | 132 | 15 | 39 | 284 | 793 | 1077 | 6.51e-13 | 72.8 |
| MS.gene69958.t1 | MTR_3g058790 | 42.373 | 118 | 42 | 3 | 2 | 119 | 773 | 864 | 8.90e-13 | 72.0 |
| MS.gene69958.t1 | MTR_0350s0040 | 30.932 | 236 | 103 | 7 | 2 | 188 | 207 | 431 | 1.92e-12 | 70.1 |
| MS.gene69958.t1 | MTR_8g018260 | 24.265 | 408 | 206 | 18 | 39 | 371 | 793 | 1172 | 2.02e-12 | 71.2 |
| MS.gene69958.t1 | MTR_8g105820 | 28.512 | 242 | 115 | 8 | 33 | 225 | 735 | 967 | 1.14e-11 | 68.6 |
| MS.gene69958.t1 | MTR_8g017945 | 45.614 | 114 | 59 | 2 | 2 | 115 | 235 | 345 | 1.17e-11 | 68.2 |
| MS.gene69958.t1 | MTR_3g012470 | 29.412 | 238 | 143 | 9 | 5 | 234 | 758 | 978 | 4.78e-11 | 66.6 |
| MS.gene69958.t1 | MTR_3g012470 | 29.412 | 238 | 143 | 9 | 5 | 234 | 758 | 978 | 7.40e-11 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 113 sgRNAs with CRISPR-Local
Find 156 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTTAATACTCTGGTCCTTTC+TGG | 0.017541 | 3.4:-75638338 | MS.gene69958:CDS |
| ATCTTCAACTTTCTTCTTAT+TGG | 0.040976 | 3.4:+75636068 | None:intergenic |
| CCCCTATGTCTTTGGAATTT+TGG | 0.093982 | 3.4:+75637757 | None:intergenic |
| GAGATATTTCAGAAATAATT+TGG | 0.131185 | 3.4:+75636313 | None:intergenic |
| ATTCTTCTCATCATAGTTAT+TGG | 0.135204 | 3.4:+75637497 | None:intergenic |
| AAACTTTCTAGTTCGGAAAT+TGG | 0.188771 | 3.4:+75636699 | None:intergenic |
| ATAGCATGATGAAGCTTAAT+AGG | 0.194421 | 3.4:+75636474 | None:intergenic |
| CCAATCAACTTTAACCATAT+TGG | 0.228631 | 3.4:-75638267 | MS.gene69958:CDS |
| GATAATAAACCAATGCTATC+AGG | 0.249057 | 3.4:+75638212 | None:intergenic |
| CTTCCAGAATCTGGTTTATA+CGG | 0.251338 | 3.4:-75637846 | MS.gene69958:CDS |
| GTCACTTATATCAAAGAAAT+TGG | 0.251927 | 3.4:-75636358 | MS.gene69958:CDS |
| TGAAAATTTAGAAACCCTTT+TGG | 0.272716 | 3.4:-75636589 | MS.gene69958:CDS |
| TTAGGGATCATGTGTTTCTT+TGG | 0.288974 | 3.4:-75637701 | MS.gene69958:CDS |
| AGAAAATTGTTGCAACTTAG+AGG | 0.306793 | 3.4:-75638240 | MS.gene69958:CDS |
| TGGGTAATTTACATTTGGTT+AGG | 0.308253 | 3.4:-75637719 | MS.gene69958:CDS |
| TTAATACTCTGGTCCTTTCT+GGG | 0.310777 | 3.4:-75638337 | MS.gene69958:CDS |
| CTCTTTGGGTAATTTACATT+TGG | 0.312348 | 3.4:-75637724 | MS.gene69958:CDS |
| GTTGGGAATCTTCCAGAATC+TGG | 0.317794 | 3.4:-75637855 | MS.gene69958:CDS |
| AACTTTCTTCTTATTGGATA+AGG | 0.321389 | 3.4:+75636074 | None:intergenic |
| GATGAAGCTTAATAGGGATT+TGG | 0.323065 | 3.4:+75636481 | None:intergenic |
| TGTCTAACCTTAGTTCTTTG+AGG | 0.329934 | 3.4:+75638117 | None:intergenic |
| TCTTCGTCTCACATCTTTGT+AGG | 0.342298 | 3.4:-75637354 | MS.gene69958:intron |
| CTTGATGCAATTTCTTAGTC+TGG | 0.342860 | 3.4:+75637427 | None:intergenic |
| TCAAGAATAGCTGCAACATT+TGG | 0.348618 | 3.4:+75636609 | None:intergenic |
| TGAATGGGTTGATCTTAAAA+AGG | 0.349990 | 3.4:-75636046 | MS.gene69958:CDS |
| GAGTTGCAAGTCACAGAAAT+TGG | 0.350910 | 3.4:-75637474 | MS.gene69958:CDS |
| AGAAATAATTTGGGATTCTC+TGG | 0.351183 | 3.4:+75636323 | None:intergenic |
| AATTTGGGATTCTCTGGCTT+TGG | 0.351977 | 3.4:+75636329 | None:intergenic |
| AAACCGTATAAACCAGATTC+TGG | 0.353356 | 3.4:+75637843 | None:intergenic |
| CCAATATGGTTAAAGTTGAT+TGG | 0.356623 | 3.4:+75638267 | None:intergenic |
| CTTTGGTATCTTGACATTAG+AGG | 0.364402 | 3.4:-75637684 | MS.gene69958:CDS |
| TTTAAGACAGTCTTACATTC+AGG | 0.365125 | 3.4:+75636132 | None:intergenic |
| TAGCTGCAACATTTGGATCT+TGG | 0.376069 | 3.4:+75636616 | None:intergenic |
| TGGTCAACAGAGAGGATAAT+AGG | 0.376338 | 3.4:-75637555 | MS.gene69958:CDS |
| TATCATCATTGCAACGCTTA+AGG | 0.377504 | 3.4:-75638193 | MS.gene69958:CDS |
| CTGACACCACTTCAATAGTC+TGG | 0.389948 | 3.4:+75636820 | None:intergenic |
| CGCTTAGGAACTTGAGATCC+AGG | 0.397868 | 3.4:+75637933 | None:intergenic |
| AGATATTTCAGAAATAATTT+GGG | 0.403733 | 3.4:+75636314 | None:intergenic |
| AGAAGAAAGTTGAAGATGAA+TGG | 0.405088 | 3.4:-75636062 | MS.gene69958:CDS |
| AATTGAAAGGACATGTTGTA+AGG | 0.406787 | 3.4:+75637603 | None:intergenic |
| CGCACCTTGAATCAGACTCT+TGG | 0.410192 | 3.4:-75636725 | MS.gene69958:CDS |
| ATGTAACAAACTTTCTAGTT+CGG | 0.410472 | 3.4:+75636692 | None:intergenic |
| GTTGTGCAACCTCTGTCTTA+AGG | 0.418073 | 3.4:+75636163 | None:intergenic |
| GGGTAATTTACATTTGGTTA+GGG | 0.419517 | 3.4:-75637718 | MS.gene69958:CDS |
| CAACATCACTTGCTCATTGT+TGG | 0.429003 | 3.4:+75636782 | None:intergenic |
| TTTCAAGTGGTGTCTCCAAA+AGG | 0.431609 | 3.4:+75636574 | None:intergenic |
| AAGAACACAGACTGAGGATA+TGG | 0.438714 | 3.4:+75637957 | None:intergenic |
| AGACTGTCTTAAAGAATTTG+AGG | 0.444655 | 3.4:-75636122 | MS.gene69958:CDS |
| CCTCAGTCTGTGTTCTTCCC+TGG | 0.447143 | 3.4:-75637951 | MS.gene69958:CDS |
| TAGCATGATGAAGCTTAATA+GGG | 0.448115 | 3.4:+75636475 | None:intergenic |
| GAAGAAAGTTGAAGATGAAT+GGG | 0.452327 | 3.4:-75636061 | MS.gene69958:CDS |
| ACCAAAATTCCAAAGACATA+GGG | 0.466009 | 3.4:-75637758 | MS.gene69958:CDS |
| TTCAAGTGGTGTCTCCAAAA+GGG | 0.466203 | 3.4:+75636575 | None:intergenic |
| CAACTTCTGCAGTAACAGTT+GGG | 0.478176 | 3.4:-75637872 | MS.gene69958:CDS |
| AGTTAGTTCTGTTAATACTC+TGG | 0.481386 | 3.4:-75638348 | MS.gene69958:CDS |
| GCACCTTGAATCAGACTCTT+GGG | 0.482241 | 3.4:-75636724 | MS.gene69958:CDS |
| GCTTCACACATCTCTTTGCA+TGG | 0.494158 | 3.4:-75637657 | MS.gene69958:CDS |
| AAGTTGATTGGCATGCTTTG+AGG | 0.494694 | 3.4:+75638279 | None:intergenic |
| AAGTTAAGAACTTTCATTGA+AGG | 0.500069 | 3.4:-75636447 | MS.gene69958:CDS |
| GCTTAGAGACAAGGTGAAGA+GGG | 0.500547 | 3.4:-75636217 | MS.gene69958:CDS |
| GAACGAAAACTAAATCGCTT+AGG | 0.503757 | 3.4:+75637918 | None:intergenic |
| GAGGCACTCCCTGATAGCAT+TGG | 0.504824 | 3.4:-75638221 | MS.gene69958:CDS |
| TGTTACTGACCTTACTACAT+TGG | 0.505042 | 3.4:-75638012 | MS.gene69958:CDS |
| AGGTTTCGATCTATGTTCAA+AGG | 0.505111 | 3.4:+75637983 | None:intergenic |
| CAGAGAGGATAATAGGTTGT+GGG | 0.506101 | 3.4:-75637548 | MS.gene69958:CDS |
| TTAACAACCTCAAAGAACTA+AGG | 0.506611 | 3.4:-75638124 | MS.gene69958:CDS |
| TCAACTTCTGCAGTAACAGT+TGG | 0.509851 | 3.4:-75637873 | MS.gene69958:CDS |
| TAAGTTGTTTGCACCCAGAA+AGG | 0.510236 | 3.4:+75638324 | None:intergenic |
| GACTGTCTTAAAGAATTTGA+GGG | 0.512530 | 3.4:-75636121 | MS.gene69958:CDS |
| AGTCTGGTTTGAGTCCTTGT+TGG | 0.513474 | 3.4:+75636836 | None:intergenic |
| GATTCTTCTAAGTGATAAAG+TGG | 0.515425 | 3.4:+75637519 | None:intergenic |
| GAGTTAGAGCAAAAGTTAAG+TGG | 0.519212 | 3.4:-75636249 | MS.gene69958:CDS |
| AGTTAAGAACTTTCATTGAA+GGG | 0.520236 | 3.4:-75636446 | MS.gene69958:CDS |
| ATGACCAACTGCTTCAATCA+AGG | 0.521862 | 3.4:-75636380 | MS.gene69958:CDS |
| GACCAAAATTCCAAAGACAT+AGG | 0.522607 | 3.4:-75637759 | MS.gene69958:CDS |
| AGACAAGGTGAAGAGGGAGT+TGG | 0.525083 | 3.4:-75636211 | MS.gene69958:CDS |
| TTGAAAACGAGGAGGGAGAA+TGG | 0.526247 | 3.4:-75637575 | MS.gene69958:CDS |
| GGTTTCGATCTATGTTCAAA+GGG | 0.526373 | 3.4:+75637984 | None:intergenic |
| AGGGTATGTCCAATGTAGTA+AGG | 0.526392 | 3.4:+75638003 | None:intergenic |
| CATTTGATTTAGAAAGAACC+AGG | 0.527864 | 3.4:+75637811 | None:intergenic |
| AGCTTAGAGACAAGGTGAAG+AGG | 0.531645 | 3.4:-75636218 | MS.gene69958:CDS |
| ACAGAGAGGATAATAGGTTG+TGG | 0.532392 | 3.4:-75637549 | MS.gene69958:CDS |
| AGACAGAGGTTGCACAACAT+TGG | 0.540693 | 3.4:-75636158 | MS.gene69958:CDS |
| ATAATAAACCAATGCTATCA+GGG | 0.542656 | 3.4:+75638213 | None:intergenic |
| CCAAAATTCCAAAGACATAG+GGG | 0.543509 | 3.4:-75637757 | MS.gene69958:CDS |
| GTTAAGAACTTTCATTGAAG+GGG | 0.548370 | 3.4:-75636445 | MS.gene69958:CDS |
| ACAGACTGAGGATATGGTTG+AGG | 0.554525 | 3.4:+75637963 | None:intergenic |
| GATCCCAAGAGTCTGATTCA+AGG | 0.562385 | 3.4:+75636721 | None:intergenic |
| CAACAATGAGCAAGTGATGT+TGG | 0.576162 | 3.4:-75636781 | MS.gene69958:CDS |
| CAAATGTAAATTACCCAAAG+AGG | 0.578852 | 3.4:+75637725 | None:intergenic |
| GTTCTGAAGAAAATTATCAT+CGG | 0.595167 | 3.4:-75637899 | MS.gene69958:CDS |
| GTGACCTTGATTGAAGCAGT+TGG | 0.600403 | 3.4:+75636376 | None:intergenic |
| TTGCAACGCTTAAGGCTAAG+TGG | 0.604635 | 3.4:-75638185 | MS.gene69958:CDS |
| TTGATTGGCATGCTTTGAGG+TGG | 0.605996 | 3.4:+75638282 | None:intergenic |
| ATCTTATCATATAAATGAAG+AGG | 0.608747 | 3.4:-75637451 | MS.gene69958:CDS |
| ATTATTTCTGAAATATCTCG+AGG | 0.613950 | 3.4:-75636309 | MS.gene69958:CDS |
| CTCAAACCAGACTATTGAAG+TGG | 0.617434 | 3.4:-75636826 | MS.gene69958:CDS |
| CAAGAGTCTGATTCAAGGTG+CGG | 0.618203 | 3.4:+75636726 | None:intergenic |
| TCTGAAGAGATAATTTCAAG+TGG | 0.620346 | 3.4:+75636561 | None:intergenic |
| CTAAACAATCGCAGTGACGG+TGG | 0.622593 | 3.4:+75638056 | None:intergenic |
| GCACTAAACAATCGCAGTGA+CGG | 0.632920 | 3.4:+75638053 | None:intergenic |
| TCTTGTTAAGCTTAGAGACA+AGG | 0.633307 | 3.4:-75636226 | MS.gene69958:CDS |
| ATTGTTGAGTCAGCGCACAT+AGG | 0.636287 | 3.4:+75636651 | None:intergenic |
| TGAAATTATCTCTTCAGACA+AGG | 0.637650 | 3.4:-75636556 | MS.gene69958:CDS |
| CACTGTAATAGCTTCCAACA+AGG | 0.638332 | 3.4:-75636850 | MS.gene69958:intron |
| AGGGAGAATGGTCAACAGAG+AGG | 0.643123 | 3.4:-75637563 | MS.gene69958:CDS |
| GCTTAGGAACTTGAGATCCA+GGG | 0.647588 | 3.4:+75637934 | None:intergenic |
| AGAGAGGATAATAGGTTGTG+GGG | 0.647987 | 3.4:-75637547 | MS.gene69958:CDS |
| CCAGGGAAGAACACAGACTG+AGG | 0.657885 | 3.4:+75637951 | None:intergenic |
| AAATAGAAACCTTAAGACAG+AGG | 0.675361 | 3.4:-75636172 | MS.gene69958:CDS |
| TGGAAGCTCAGACACAGACA+CGG | 0.683675 | 3.4:+75638076 | None:intergenic |
| CAAAATTCCAAAGACATAGG+GGG | 0.687986 | 3.4:-75637756 | MS.gene69958:CDS |
| AAATCAAATGACAAGTACAG+AGG | 0.693240 | 3.4:-75637798 | MS.gene69958:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGATATTTCAGAAATAATTT+GGG | + | chr3.4:75638065-75638084 | None:intergenic | 15.0% |
| !!! | TTGTTAAGATTTTTTATGTT+TGG | + | chr3.4:75636239-75636258 | None:intergenic | 15.0% |
| !! | AAGTTCTGTCAAAAAATTAA+AGG | - | chr3.4:75637238-75637257 | MS.gene69958:intron | 20.0% |
| !! | ATCTTATCATATAAATGAAG+AGG | - | chr3.4:75636925-75636944 | MS.gene69958:intron | 20.0% |
| !! | ATTACATATCCTATAATTAG+AGG | + | chr3.4:75637463-75637482 | None:intergenic | 20.0% |
| !! | GAGATATTTCAGAAATAATT+TGG | + | chr3.4:75638066-75638085 | None:intergenic | 20.0% |
| !! | GATATAAAATTTCAATCAAG+TGG | + | chr3.4:75637423-75637442 | None:intergenic | 20.0% |
| !! | GATATGTAATAAAATTTCCT+TGG | - | chr3.4:75637473-75637492 | MS.gene69958:CDS | 20.0% |
| !! | TTACATATCCTATAATTAGA+GGG | + | chr3.4:75637462-75637481 | None:intergenic | 20.0% |
| !!! | ACAATTTTCTAAATCCAATA+TGG | + | chr3.4:75636126-75636145 | None:intergenic | 20.0% |
| !!! | GAAAAAGTTCCATAAAATTT+TGG | - | chr3.4:75637850-75637869 | MS.gene69958:CDS | 20.0% |
| ! | AAAAGATTCAATAAAGACCA+AGG | + | chr3.4:75637493-75637512 | None:intergenic | 25.0% |
| ! | AACTTTCTTCTTATTGGATA+AGG | + | chr3.4:75638305-75638324 | None:intergenic | 25.0% |
| ! | AAGTTAAGAACTTTCATTGA+AGG | - | chr3.4:75637929-75637948 | MS.gene69958:CDS | 25.0% |
| ! | AGTTAAGAACTTTCATTGAA+GGG | - | chr3.4:75637930-75637949 | MS.gene69958:CDS | 25.0% |
| ! | ATAATAAACCAATGCTATCA+GGG | + | chr3.4:75636166-75636185 | None:intergenic | 25.0% |
| ! | ATCTTCAACTTTCTTCTTAT+TGG | + | chr3.4:75638311-75638330 | None:intergenic | 25.0% |
| ! | ATGCAATTTAGATGAAAATG+AGG | + | chr3.4:75637328-75637347 | None:intergenic | 25.0% |
| ! | ATTCTTCTCATCATAGTTAT+TGG | + | chr3.4:75636882-75636901 | None:intergenic | 25.0% |
| ! | ATTTAGATGAAAATGAGGAA+TGG | + | chr3.4:75637323-75637342 | None:intergenic | 25.0% |
| ! | GTCACTTATATCAAAGAAAT+TGG | - | chr3.4:75638018-75638037 | MS.gene69958:CDS | 25.0% |
| ! | TTGCATTAAAGATGAAAAAC+TGG | - | chr3.4:75636985-75637004 | MS.gene69958:intron | 25.0% |
| !! | CAAGTTGTTCCAAAATTTTA+TGG | + | chr3.4:75637862-75637881 | None:intergenic | 25.0% |
| !! | TGAAAATTTAGAAACCCTTT+TGG | - | chr3.4:75637787-75637806 | MS.gene69958:CDS | 25.0% |
| !!! | ATGTAACAAACTTTCTAGTT+CGG | + | chr3.4:75637687-75637706 | None:intergenic | 25.0% |
| !!! | ATTATTTCTGAAATATCTCG+AGG | - | chr3.4:75638067-75638086 | MS.gene69958:CDS | 25.0% |
| !!! | CATAAAATTTTGGAACAACT+TGG | - | chr3.4:75637860-75637879 | MS.gene69958:CDS | 25.0% |
| !!! | GTTCTGAAGAAAATTATCAT+CGG | - | chr3.4:75636477-75636496 | MS.gene69958:CDS | 25.0% |
| !!! | GTTTTCAAGTGAAAATTGAA+AGG | + | chr3.4:75636789-75636808 | None:intergenic | 25.0% |
| !!! | TTTTTTCTAGAGCAATCAAA+AGG | - | chr3.4:75637095-75637114 | MS.gene69958:intron | 25.0% |
| AAATAGAAACCTTAAGACAG+AGG | - | chr3.4:75638204-75638223 | MS.gene69958:CDS | 30.0% | |
| AAATCAAATGACAAGTACAG+AGG | - | chr3.4:75636578-75636597 | MS.gene69958:CDS | 30.0% | |
| AATTGAAAGGACATGTTGTA+AGG | + | chr3.4:75636776-75636795 | None:intergenic | 30.0% | |
| ACCAAAATTCCAAAGACATA+GGG | - | chr3.4:75636618-75636637 | MS.gene69958:CDS | 30.0% | |
| AGAAAATTGTTGCAACTTAG+AGG | - | chr3.4:75636136-75636155 | MS.gene69958:CDS | 30.0% | |
| AGAAGAAAGTTGAAGATGAA+TGG | - | chr3.4:75638314-75638333 | MS.gene69958:CDS | 30.0% | |
| AGACTGTCTTAAAGAATTTG+AGG | - | chr3.4:75638254-75638273 | MS.gene69958:CDS | 30.0% | |
| ATAGCATGATGAAGCTTAAT+AGG | + | chr3.4:75637905-75637924 | None:intergenic | 30.0% | |
| ATATCCTATAATTAGAGGGA+GGG | + | chr3.4:75637458-75637477 | None:intergenic | 30.0% | |
| CAAATGTAAATTACCCAAAG+AGG | + | chr3.4:75636654-75636673 | None:intergenic | 30.0% | |
| CCAATCAACTTTAACCATAT+TGG | - | chr3.4:75636109-75636128 | MS.gene69958:CDS | 30.0% | |
| CTCTTTGGGTAATTTACATT+TGG | - | chr3.4:75636652-75636671 | MS.gene69958:CDS | 30.0% | |
| GAAGAAAGTTGAAGATGAAT+GGG | - | chr3.4:75638315-75638334 | MS.gene69958:CDS | 30.0% | |
| GACTGTCTTAAAGAATTTGA+GGG | - | chr3.4:75638255-75638274 | MS.gene69958:CDS | 30.0% | |
| GATAATAAACCAATGCTATC+AGG | + | chr3.4:75636167-75636186 | None:intergenic | 30.0% | |
| GATTCTTCTAAGTGATAAAG+TGG | + | chr3.4:75636860-75636879 | None:intergenic | 30.0% | |
| GGGTAATTTACATTTGGTTA+GGG | - | chr3.4:75636658-75636677 | MS.gene69958:CDS | 30.0% | |
| GTTAAGAACTTTCATTGAAG+GGG | - | chr3.4:75637931-75637950 | MS.gene69958:CDS | 30.0% | |
| TAGCATGATGAAGCTTAATA+GGG | + | chr3.4:75637904-75637923 | None:intergenic | 30.0% | |
| TCTGAAGAGATAATTTCAAG+TGG | + | chr3.4:75637818-75637837 | None:intergenic | 30.0% | |
| TGAAATTATCTCTTCAGACA+AGG | - | chr3.4:75637820-75637839 | MS.gene69958:CDS | 30.0% | |
| TGGGTAATTTACATTTGGTT+AGG | - | chr3.4:75636657-75636676 | MS.gene69958:CDS | 30.0% | |
| TTAACAACCTCAAAGAACTA+AGG | - | chr3.4:75636252-75636271 | MS.gene69958:CDS | 30.0% | |
| ! | AGAAATAATTTGGGATTCTC+TGG | + | chr3.4:75638056-75638075 | None:intergenic | 30.0% |
| ! | CATTTGATTTAGAAAGAACC+AGG | + | chr3.4:75636568-75636587 | None:intergenic | 30.0% |
| ! | CCAATATGGTTAAAGTTGAT+TGG | + | chr3.4:75636112-75636131 | None:intergenic | 30.0% |
| ! | TCAATTTTCACTTGAAAACG+AGG | - | chr3.4:75636790-75636809 | MS.gene69958:CDS | 30.0% |
| ! | TTTAAGACAGTCTTACATTC+AGG | + | chr3.4:75638247-75638266 | None:intergenic | 30.0% |
| ! | TTTCAATCAAGTGGTCATTT+TGG | + | chr3.4:75637414-75637433 | None:intergenic | 30.0% |
| !! | AAACTTTCTAGTTCGGAAAT+TGG | + | chr3.4:75637680-75637699 | None:intergenic | 30.0% |
| !! | AGTTAGTTCTGTTAATACTC+TGG | - | chr3.4:75636028-75636047 | MS.gene69958:CDS | 30.0% |
| !! | TGAATGGGTTGATCTTAAAA+AGG | - | chr3.4:75638330-75638349 | MS.gene69958:CDS | 30.0% |
| !!! | TAGGGATTTGGTTTTCAAAT+TGG | + | chr3.4:75637886-75637905 | None:intergenic | 30.0% |
| AAACCGTATAAACCAGATTC+TGG | + | chr3.4:75636536-75636555 | None:intergenic | 35.0% | |
| AAATACAAAAACGTGGAGGA+TGG | - | chr3.4:75636744-75636763 | MS.gene69958:CDS | 35.0% | |
| AATACAAAAACGTGGAGGAT+GGG | - | chr3.4:75636745-75636764 | MS.gene69958:CDS | 35.0% | |
| AGGTTTCGATCTATGTTCAA+AGG | + | chr3.4:75636396-75636415 | None:intergenic | 35.0% | |
| CAAAATTCCAAAGACATAGG+GGG | - | chr3.4:75636620-75636639 | MS.gene69958:CDS | 35.0% | |
| CATATCCTATAATTAGAGGG+AGG | + | chr3.4:75637459-75637478 | None:intergenic | 35.0% | |
| CCAAAATTCCAAAGACATAG+GGG | - | chr3.4:75636619-75636638 | MS.gene69958:CDS | 35.0% | |
| CTTGATGCAATTTCTTAGTC+TGG | + | chr3.4:75636952-75636971 | None:intergenic | 35.0% | |
| CTTTGGTATCTTGACATTAG+AGG | - | chr3.4:75636692-75636711 | MS.gene69958:CDS | 35.0% | |
| GAACGAAAACTAAATCGCTT+AGG | + | chr3.4:75636461-75636480 | None:intergenic | 35.0% | |
| GACCAAAATTCCAAAGACAT+AGG | - | chr3.4:75636617-75636636 | MS.gene69958:CDS | 35.0% | |
| GAGTTAGAGCAAAAGTTAAG+TGG | - | chr3.4:75638127-75638146 | MS.gene69958:CDS | 35.0% | |
| GATGAAGCTTAATAGGGATT+TGG | + | chr3.4:75637898-75637917 | None:intergenic | 35.0% | |
| GGTTTCGATCTATGTTCAAA+GGG | + | chr3.4:75636395-75636414 | None:intergenic | 35.0% | |
| TATCATCATTGCAACGCTTA+AGG | - | chr3.4:75636183-75636202 | MS.gene69958:CDS | 35.0% | |
| TATCCTATAATTAGAGGGAG+GGG | + | chr3.4:75637457-75637476 | None:intergenic | 35.0% | |
| TCAAGAATAGCTGCAACATT+TGG | + | chr3.4:75637770-75637789 | None:intergenic | 35.0% | |
| TCTTGTTAAGCTTAGAGACA+AGG | - | chr3.4:75638150-75638169 | MS.gene69958:CDS | 35.0% | |
| TGTTACTGACCTTACTACAT+TGG | - | chr3.4:75636364-75636383 | MS.gene69958:CDS | 35.0% | |
| TTAATACTCTGGTCCTTTCT+GGG | - | chr3.4:75636039-75636058 | MS.gene69958:CDS | 35.0% | |
| TTAGGGATCATGTGTTTCTT+TGG | - | chr3.4:75636675-75636694 | MS.gene69958:CDS | 35.0% | |
| ! | ATCCTCCACGTTTTTGTATT+TGG | + | chr3.4:75636745-75636764 | None:intergenic | 35.0% |
| ! | ATTTTCACTTGAAAACGAGG+AGG | - | chr3.4:75636793-75636812 | MS.gene69958:CDS | 35.0% |
| ! | CTTCCAGAATCTGGTTTATA+CGG | - | chr3.4:75636530-75636549 | MS.gene69958:CDS | 35.0% |
| ! | GTGAGACGAAGATGTTTTAT+TGG | + | chr3.4:75637014-75637033 | None:intergenic | 35.0% |
| ! | TGTCTAACCTTAGTTCTTTG+AGG | + | chr3.4:75636262-75636281 | None:intergenic | 35.0% |
| ! | TTTTCACTTGAAAACGAGGA+GGG | - | chr3.4:75636794-75636813 | MS.gene69958:CDS | 35.0% |
| AAGAACACAGACTGAGGATA+TGG | + | chr3.4:75636422-75636441 | None:intergenic | 40.0% | |
| ACAGAGAGGATAATAGGTTG+TGG | - | chr3.4:75636827-75636846 | MS.gene69958:CDS | 40.0% | |
| AGAGAGGATAATAGGTTGTG+GGG | - | chr3.4:75636829-75636848 | MS.gene69958:CDS | 40.0% | |
| AGGGTATGTCCAATGTAGTA+AGG | + | chr3.4:75636376-75636395 | None:intergenic | 40.0% | |
| ATCCTATAATTAGAGGGAGG+GGG | + | chr3.4:75637456-75637475 | None:intergenic | 40.0% | |
| ATGACCAACTGCTTCAATCA+AGG | - | chr3.4:75637996-75638015 | MS.gene69958:CDS | 40.0% | |
| CAACAATGAGCAAGTGATGT+TGG | - | chr3.4:75637595-75637614 | MS.gene69958:CDS | 40.0% | |
| CAACATCACTTGCTCATTGT+TGG | + | chr3.4:75637597-75637616 | None:intergenic | 40.0% | |
| CAACTTCTGCAGTAACAGTT+GGG | - | chr3.4:75636504-75636523 | MS.gene69958:CDS | 40.0% | |
| CACTGTAATAGCTTCCAACA+AGG | - | chr3.4:75637526-75637545 | MS.gene69958:CDS | 40.0% | |
| CAGAGAGGATAATAGGTTGT+GGG | - | chr3.4:75636828-75636847 | MS.gene69958:CDS | 40.0% | |
| CATGGCCAAATACAAAAACG+TGG | - | chr3.4:75636737-75636756 | MS.gene69958:CDS | 40.0% | |
| CCAACAGCCACAAATACAAA+TGG | - | chr3.4:75637170-75637189 | MS.gene69958:intron | 40.0% | |
| CCATTTGTATTTGTGGCTGT+TGG | + | chr3.4:75637173-75637192 | None:intergenic | 40.0% | |
| CGTGCAACCATTTGTATTTG+TGG | + | chr3.4:75637180-75637199 | None:intergenic | 40.0% | |
| CTCAAACCAGACTATTGAAG+TGG | - | chr3.4:75637550-75637569 | MS.gene69958:CDS | 40.0% | |
| GAGAAAACCACAAAGTTTCG+AGG | - | chr3.4:75637199-75637218 | MS.gene69958:intron | 40.0% | |
| GAGTTGCAAGTCACAGAAAT+TGG | - | chr3.4:75636902-75636921 | MS.gene69958:intron | 40.0% | |
| GTTAATACTCTGGTCCTTTC+TGG | - | chr3.4:75636038-75636057 | MS.gene69958:CDS | 40.0% | |
| TAAGTTGTTTGCACCCAGAA+AGG | + | chr3.4:75636055-75636074 | None:intergenic | 40.0% | |
| TAGCTGCAACATTTGGATCT+TGG | + | chr3.4:75637763-75637782 | None:intergenic | 40.0% | |
| TCAACTTCTGCAGTAACAGT+TGG | - | chr3.4:75636503-75636522 | MS.gene69958:CDS | 40.0% | |
| TCTTCGTCTCACATCTTTGT+AGG | - | chr3.4:75637022-75637041 | MS.gene69958:intron | 40.0% | |
| TGGTCAACAGAGAGGATAAT+AGG | - | chr3.4:75636821-75636840 | MS.gene69958:CDS | 40.0% | |
| TTCAAGTGGTGTCTCCAAAA+GGG | + | chr3.4:75637804-75637823 | None:intergenic | 40.0% | |
| ! | AAGTTGATTGGCATGCTTTG+AGG | + | chr3.4:75636100-75636119 | None:intergenic | 40.0% |
| ! | AATTTGGGATTCTCTGGCTT+TGG | + | chr3.4:75638050-75638069 | None:intergenic | 40.0% |
| ! | TTTCAAGTGGTGTCTCCAAA+AGG | + | chr3.4:75637805-75637824 | None:intergenic | 40.0% |
| !! | ATACGGTTTTGTGTTTTGCC+TGG | - | chr3.4:75636547-75636566 | MS.gene69958:CDS | 40.0% |
| !!! | CCCCTATGTCTTTGGAATTT+TGG | + | chr3.4:75636622-75636641 | None:intergenic | 40.0% |
| AACCCCCTCCCTCTAATTAT+AGG | - | chr3.4:75637451-75637470 | MS.gene69958:CDS | 45.0% | |
| ACAGACTGAGGATATGGTTG+AGG | + | chr3.4:75636416-75636435 | None:intergenic | 45.0% | |
| ACCACAAAGTTTCGAGGAAG+AGG | - | chr3.4:75637205-75637224 | MS.gene69958:intron | 45.0% | |
| AGACAGAGGTTGCACAACAT+TGG | - | chr3.4:75638218-75638237 | MS.gene69958:CDS | 45.0% | |
| AGCTTAGAGACAAGGTGAAG+AGG | - | chr3.4:75638158-75638177 | MS.gene69958:CDS | 45.0% | |
| ATTGTTGAGTCAGCGCACAT+AGG | + | chr3.4:75637728-75637747 | None:intergenic | 45.0% | |
| CAAGAGTCTGATTCAAGGTG+CGG | + | chr3.4:75637653-75637672 | None:intergenic | 45.0% | |
| CTGACACCACTTCAATAGTC+TGG | + | chr3.4:75637559-75637578 | None:intergenic | 45.0% | |
| GATCCCAAGAGTCTGATTCA+AGG | + | chr3.4:75637658-75637677 | None:intergenic | 45.0% | |
| GCACCTTGAATCAGACTCTT+GGG | - | chr3.4:75637652-75637671 | MS.gene69958:CDS | 45.0% | |
| GCACTAAACAATCGCAGTGA+CGG | + | chr3.4:75636326-75636345 | None:intergenic | 45.0% | |
| GCTTAGAGACAAGGTGAAGA+GGG | - | chr3.4:75638159-75638178 | MS.gene69958:CDS | 45.0% | |
| GCTTAGGAACTTGAGATCCA+GGG | + | chr3.4:75636445-75636464 | None:intergenic | 45.0% | |
| GCTTCACACATCTCTTTGCA+TGG | - | chr3.4:75636719-75636738 | MS.gene69958:CDS | 45.0% | |
| GGCCAAATACAAAAACGTGG+AGG | - | chr3.4:75636740-75636759 | MS.gene69958:CDS | 45.0% | |
| GTATTTGTGGCTGTTGGAAC+TGG | + | chr3.4:75637167-75637186 | None:intergenic | 45.0% | |
| GTGACCTTGATTGAAGCAGT+TGG | + | chr3.4:75638003-75638022 | None:intergenic | 45.0% | |
| GTTGGGAATCTTCCAGAATC+TGG | - | chr3.4:75636521-75636540 | MS.gene69958:CDS | 45.0% | |
| GTTGTGCAACCTCTGTCTTA+AGG | + | chr3.4:75638216-75638235 | None:intergenic | 45.0% | |
| TCCTCTTCCTCGAAACTTTG+TGG | + | chr3.4:75637209-75637228 | None:intergenic | 45.0% | |
| TTGAAAACGAGGAGGGAGAA+TGG | - | chr3.4:75636801-75636820 | MS.gene69958:CDS | 45.0% | |
| TTGCAACGCTTAAGGCTAAG+TGG | - | chr3.4:75636191-75636210 | MS.gene69958:CDS | 45.0% | |
| ! | AGTCTGGTTTGAGTCCTTGT+TGG | + | chr3.4:75637543-75637562 | None:intergenic | 45.0% |
| !! | TTGATTGGCATGCTTTGAGG+TGG | + | chr3.4:75636097-75636116 | None:intergenic | 45.0% |
| AGACAAGGTGAAGAGGGAGT+TGG | - | chr3.4:75638165-75638184 | MS.gene69958:CDS | 50.0% | |
| AGCTCTTCCCCCTATGTCTT+TGG | + | chr3.4:75636630-75636649 | None:intergenic | 50.0% | |
| AGGGAGAATGGTCAACAGAG+AGG | - | chr3.4:75636813-75636832 | MS.gene69958:CDS | 50.0% | |
| CGCACCTTGAATCAGACTCT+TGG | - | chr3.4:75637651-75637670 | MS.gene69958:CDS | 50.0% | |
| CGCTTAGGAACTTGAGATCC+AGG | + | chr3.4:75636446-75636465 | None:intergenic | 50.0% | |
| CTAAACAATCGCAGTGACGG+TGG | + | chr3.4:75636323-75636342 | None:intergenic | 50.0% | |
| TGGAAGCTCAGACACAGACA+CGG | + | chr3.4:75636303-75636322 | None:intergenic | 50.0% | |
| AGGGGGAAGAGCTACCTCTT+TGG | - | chr3.4:75636637-75636656 | MS.gene69958:CDS | 55.0% | |
| CCAGGGAAGAACACAGACTG+AGG | + | chr3.4:75636428-75636447 | None:intergenic | 55.0% | |
| CCTCAGTCTGTGTTCTTCCC+TGG | - | chr3.4:75636425-75636444 | MS.gene69958:CDS | 55.0% | |
| GGGGGAAGAGCTACCTCTTT+GGG | - | chr3.4:75636638-75636657 | MS.gene69958:CDS | 55.0% | |
| ! | GAGGCACTCCCTGATAGCAT+TGG | - | chr3.4:75636155-75636174 | MS.gene69958:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 75636023 | 75638375 | 75636023 | ID=MS.gene69958 |
| chr3.4 | mRNA | 75636023 | 75638375 | 75636023 | ID=MS.gene69958.t1;Parent=MS.gene69958 |
| chr3.4 | exon | 75637355 | 75638375 | 75637355 | ID=MS.gene69958.t1.exon1;Parent=MS.gene69958.t1 |
| chr3.4 | CDS | 75637355 | 75638375 | 75637355 | ID=cds.MS.gene69958.t1;Parent=MS.gene69958.t1 |
| chr3.4 | exon | 75636023 | 75636861 | 75636023 | ID=MS.gene69958.t1.exon2;Parent=MS.gene69958.t1 |
| chr3.4 | CDS | 75636023 | 75636861 | 75636023 | ID=cds.MS.gene69958.t1;Parent=MS.gene69958.t1 |
| Gene Sequence |
| Protein sequence |
