Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69978.t1 | RHN41825.1 | 86.2 | 586 | 81 | 0 | 1 | 586 | 3 | 588 | 7.50E-286 | 992.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69978.t1 | Q9FKY5 | 65.8 | 485 | 148 | 4 | 57 | 535 | 55 | 527 | 3.9e-182 | 639.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69978.t1 | A0A396GSS4 | 86.2 | 586 | 81 | 0 | 1 | 586 | 3 | 588 | 5.4e-286 | 992.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene04920 | MS.gene69978 | 0.809824 | 1.56E-50 | -1.69E-46 |
| MS.gene049283 | MS.gene69978 | 0.800944 | 1.14E-48 | -1.69E-46 |
| MS.gene050414 | MS.gene69978 | 0.810675 | 1.02E-50 | -1.69E-46 |
| MS.gene051043 | MS.gene69978 | 0.807579 | 4.72E-50 | -1.69E-46 |
| MS.gene052016 | MS.gene69978 | 0.825941 | 3.56E-54 | -1.69E-46 |
| MS.gene052296 | MS.gene69978 | 0.842384 | 2.67E-58 | -1.69E-46 |
| MS.gene053298 | MS.gene69978 | 0.801272 | 9.74E-49 | -1.69E-46 |
| MS.gene054892 | MS.gene69978 | 0.811862 | 5.66E-51 | -1.69E-46 |
| MS.gene054893 | MS.gene69978 | 0.812901 | 3.36E-51 | -1.69E-46 |
| MS.gene05539 | MS.gene69978 | 0.803752 | 3.00E-49 | -1.69E-46 |
| MS.gene056082 | MS.gene69978 | 0.802044 | 6.76E-49 | -1.69E-46 |
| MS.gene05985 | MS.gene69978 | -0.804024 | 2.63E-49 | -1.69E-46 |
| MS.gene061100 | MS.gene69978 | 0.803082 | 4.13E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69978.t1 | MTR_8g073130 | 92.662 | 586 | 43 | 0 | 1 | 586 | 3 | 588 | 0.0 | 1118 |
| MS.gene69978.t1 | MTR_8g073130 | 91.241 | 411 | 36 | 0 | 1 | 411 | 3 | 413 | 0.0 | 789 |
| MS.gene69978.t1 | MTR_5g024700 | 69.464 | 560 | 149 | 10 | 6 | 558 | 3 | 547 | 0.0 | 778 |
| MS.gene69978.t1 | MTR_8g073130 | 92.112 | 393 | 31 | 0 | 1 | 393 | 3 | 395 | 0.0 | 764 |
| MS.gene69978.t1 | MTR_3g115960 | 59.756 | 492 | 172 | 2 | 34 | 500 | 9 | 499 | 0.0 | 580 |
| MS.gene69978.t1 | MTR_3g115940 | 48.571 | 525 | 248 | 7 | 31 | 540 | 4 | 521 | 3.35e-173 | 502 |
| MS.gene69978.t1 | MTR_6g033330 | 47.710 | 524 | 248 | 8 | 36 | 538 | 9 | 527 | 9.19e-168 | 489 |
| MS.gene69978.t1 | MTR_3g115940 | 48.842 | 475 | 221 | 7 | 81 | 540 | 3 | 470 | 1.49e-159 | 466 |
| MS.gene69978.t1 | MTR_2g093750 | 46.063 | 508 | 250 | 6 | 31 | 523 | 3 | 501 | 5.16e-154 | 455 |
| MS.gene69978.t1 | MTR_3g115940 | 44.000 | 525 | 229 | 8 | 31 | 540 | 4 | 478 | 1.19e-144 | 428 |
| MS.gene69978.t1 | MTR_6g033330 | 48.293 | 410 | 202 | 3 | 29 | 428 | 2 | 411 | 5.85e-138 | 407 |
| MS.gene69978.t1 | MTR_3g115940 | 51.554 | 386 | 174 | 4 | 31 | 407 | 4 | 385 | 9.88e-136 | 400 |
| MS.gene69978.t1 | MTR_8g010530 | 44.589 | 462 | 235 | 4 | 36 | 476 | 12 | 473 | 3.23e-133 | 399 |
| MS.gene69978.t1 | MTR_3g118190 | 43.875 | 449 | 230 | 6 | 38 | 469 | 14 | 457 | 7.57e-128 | 385 |
| MS.gene69978.t1 | MTR_4g079140 | 38.306 | 543 | 305 | 8 | 33 | 567 | 4 | 524 | 4.31e-122 | 372 |
| MS.gene69978.t1 | MTR_2g079500 | 39.212 | 533 | 285 | 14 | 29 | 543 | 2 | 513 | 1.07e-117 | 360 |
| MS.gene69978.t1 | MTR_8g010500 | 39.958 | 473 | 247 | 6 | 54 | 500 | 28 | 489 | 2.38e-116 | 355 |
| MS.gene69978.t1 | MTR_2g028470 | 41.401 | 471 | 258 | 10 | 35 | 498 | 7 | 466 | 4.19e-113 | 346 |
| MS.gene69978.t1 | MTR_2g006280 | 36.044 | 455 | 265 | 8 | 36 | 476 | 9 | 451 | 1.97e-88 | 284 |
| MS.gene69978.t1 | MTR_5g022400 | 33.333 | 531 | 319 | 10 | 30 | 547 | 4 | 512 | 4.63e-83 | 270 |
| MS.gene69978.t1 | MTR_5g022400 | 34.625 | 387 | 235 | 7 | 30 | 403 | 4 | 385 | 7.90e-62 | 209 |
| MS.gene69978.t1 | MTR_2g030020 | 38.650 | 163 | 64 | 7 | 371 | 527 | 8 | 140 | 2.83e-21 | 91.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene69978.t1 | AT2G17480 | 62.478 | 565 | 186 | 8 | 36 | 586 | 39 | 591 | 0.0 | 705 |
| MS.gene69978.t1 | AT5G65970 | 63.551 | 535 | 178 | 5 | 7 | 535 | 4 | 527 | 0.0 | 668 |
| MS.gene69978.t1 | AT2G17430 | 61.024 | 508 | 188 | 3 | 26 | 529 | 21 | 522 | 0.0 | 640 |
| MS.gene69978.t1 | AT2G33670 | 61.991 | 442 | 161 | 4 | 36 | 476 | 15 | 450 | 0.0 | 575 |
| MS.gene69978.t1 | AT1G42560 | 60.314 | 446 | 172 | 3 | 36 | 477 | 14 | 458 | 0.0 | 547 |
| MS.gene69978.t1 | AT5G65970 | 65.160 | 376 | 119 | 3 | 7 | 381 | 4 | 368 | 0.0 | 522 |
| MS.gene69978.t1 | AT1G61560 | 50.099 | 503 | 230 | 6 | 36 | 521 | 8 | 506 | 1.67e-171 | 499 |
| MS.gene69978.t1 | AT1G61560 | 48.708 | 503 | 237 | 6 | 36 | 521 | 8 | 506 | 2.03e-162 | 476 |
| MS.gene69978.t1 | AT2G39200 | 43.694 | 563 | 281 | 7 | 36 | 582 | 6 | 548 | 1.37e-161 | 474 |
| MS.gene69978.t1 | AT1G11310 | 48.297 | 499 | 229 | 7 | 36 | 510 | 8 | 501 | 3.22e-160 | 470 |
| MS.gene69978.t1 | AT1G61560 | 52.765 | 434 | 186 | 5 | 35 | 451 | 7 | 438 | 4.48e-157 | 457 |
| MS.gene69978.t1 | AT1G42560 | 58.424 | 368 | 148 | 3 | 36 | 399 | 14 | 380 | 1.70e-145 | 425 |
| MS.gene69978.t1 | AT1G61560 | 51.582 | 411 | 180 | 5 | 35 | 428 | 7 | 415 | 1.99e-144 | 424 |
| MS.gene69978.t1 | AT1G61560 | 51.582 | 411 | 180 | 5 | 35 | 428 | 7 | 415 | 1.99e-144 | 424 |
| MS.gene69978.t1 | AT1G61560 | 48.214 | 448 | 211 | 6 | 91 | 521 | 1 | 444 | 6.83e-144 | 426 |
| MS.gene69978.t1 | AT1G61560 | 48.214 | 448 | 211 | 6 | 91 | 521 | 1 | 444 | 6.83e-144 | 426 |
| MS.gene69978.t1 | AT3G45290 | 42.151 | 465 | 258 | 2 | 26 | 490 | 7 | 460 | 1.58e-139 | 415 |
| MS.gene69978.t1 | AT1G61560 | 52.332 | 386 | 165 | 5 | 35 | 403 | 7 | 390 | 1.75e-136 | 403 |
| MS.gene69978.t1 | AT1G61560 | 52.332 | 386 | 165 | 5 | 35 | 403 | 7 | 390 | 1.75e-136 | 403 |
| MS.gene69978.t1 | AT1G61560 | 52.332 | 386 | 165 | 5 | 35 | 403 | 7 | 390 | 1.75e-136 | 403 |
| MS.gene69978.t1 | AT1G11310 | 49.153 | 413 | 188 | 5 | 36 | 428 | 8 | 418 | 2.23e-134 | 398 |
| MS.gene69978.t1 | AT1G11310 | 45.946 | 444 | 211 | 7 | 91 | 510 | 1 | 439 | 2.23e-131 | 394 |
| MS.gene69978.t1 | AT4G02600 | 42.585 | 472 | 250 | 5 | 32 | 485 | 4 | 472 | 2.83e-129 | 389 |
| MS.gene69978.t1 | AT4G02600 | 42.585 | 472 | 250 | 5 | 32 | 485 | 4 | 472 | 2.83e-129 | 389 |
| MS.gene69978.t1 | AT2G44110 | 40.000 | 500 | 288 | 6 | 32 | 528 | 2 | 492 | 8.82e-125 | 377 |
| MS.gene69978.t1 | AT2G44110 | 40.159 | 503 | 284 | 8 | 32 | 528 | 2 | 493 | 4.32e-123 | 372 |
| MS.gene69978.t1 | AT5G53760 | 38.506 | 522 | 306 | 9 | 27 | 538 | 7 | 523 | 1.46e-119 | 366 |
| MS.gene69978.t1 | AT5G53760 | 38.506 | 522 | 306 | 9 | 27 | 538 | 7 | 523 | 1.46e-119 | 366 |
| MS.gene69978.t1 | AT4G24250 | 40.726 | 496 | 248 | 10 | 25 | 504 | 1 | 466 | 1.45e-114 | 350 |
| MS.gene69978.t1 | AT1G26700 | 39.030 | 474 | 276 | 8 | 30 | 492 | 4 | 475 | 1.13e-111 | 345 |
| MS.gene69978.t1 | AT1G26700 | 39.030 | 474 | 276 | 8 | 30 | 492 | 4 | 475 | 1.13e-111 | 345 |
| MS.gene69978.t1 | AT4G24250 | 40.476 | 420 | 217 | 9 | 25 | 428 | 1 | 403 | 3.15e-94 | 295 |
| MS.gene69978.t1 | AT1G11000 | 32.150 | 535 | 325 | 8 | 35 | 542 | 8 | 531 | 3.89e-86 | 279 |
| MS.gene69978.t1 | AT1G11000 | 32.150 | 535 | 325 | 8 | 35 | 542 | 8 | 531 | 3.89e-86 | 279 |
| MS.gene69978.t1 | AT1G11000 | 34.343 | 396 | 237 | 6 | 35 | 418 | 8 | 392 | 2.15e-70 | 233 |
Find 117 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTTAATTCGCGCTTCTTTC+CGG | 0.129139 | 8.1:-32632045 | None:intergenic |
| TAACATATTTCTTGTGGATA+TGG | 0.159043 | 8.1:+32630671 | MS.gene69978:CDS |
| GATGCTTACCTTCAACATTT+AGG | 0.213554 | 8.1:-32629514 | None:intergenic |
| CAGGATGGCATGCTATGTTT+TGG | 0.214599 | 8.1:+32629619 | MS.gene69978:intron |
| GCAATAGTCAAAGTAGCTTT+AGG | 0.234184 | 8.1:+32630828 | MS.gene69978:CDS |
| TAATTGGTTGCAGATAATTT+TGG | 0.241241 | 8.1:+32630074 | MS.gene69978:intron |
| CATAATTTGCAGTATTCTTT+CGG | 0.257506 | 8.1:+32630771 | MS.gene69978:intron |
| ATAATTTGCAGTATTCTTTC+GGG | 0.277858 | 8.1:+32630772 | MS.gene69978:intron |
| TGAAAGTTAAATTTACTTCC+TGG | 0.310672 | 8.1:-32629300 | None:intergenic |
| ACTTACAATGACAGGAATTA+AGG | 0.337050 | 8.1:-32629646 | None:intergenic |
| ACAAAGGTTGTTTCTGTGTC+TGG | 0.345099 | 8.1:+32623037 | MS.gene69978:CDS |
| GCATCGCGGGAAGCTCTATA+TGG | 0.345136 | 8.1:-32624000 | None:intergenic |
| GTTACCTCCTTGCATTTGTA+AGG | 0.348157 | 8.1:-32624096 | None:intergenic |
| GCTTCTTTAGACAGTTCTAT+AGG | 0.358464 | 8.1:+32629144 | MS.gene69978:CDS |
| CAATAGTCAAAGTAGCTTTA+GGG | 0.359293 | 8.1:+32630829 | MS.gene69978:CDS |
| GAGGGCTTTGCTTGAAGCTC+TGG | 0.377031 | 8.1:+32623495 | MS.gene69978:CDS |
| ATACCTGGAACAAGGCAAAA+TGG | 0.385469 | 8.1:-32630242 | None:intergenic |
| AACATATTTCTTGTGGATAT+GGG | 0.385485 | 8.1:+32630672 | MS.gene69978:CDS |
| TTCCTTCTCCTAAATGTTGA+AGG | 0.388494 | 8.1:+32629506 | MS.gene69978:CDS |
| CAAGGGATTCCCCTTGTTCA+AGG | 0.397944 | 8.1:+32630168 | MS.gene69978:CDS |
| ATCGGTAGTGCTTCCGTCCC+TGG | 0.398088 | 8.1:-32631767 | None:intergenic |
| AGTGCTGTCACAATGCTTCT+TGG | 0.400774 | 8.1:+32624686 | MS.gene69978:CDS |
| TGTTGACAGTCCTGTCCTTT+GGG | 0.405413 | 8.1:+32629469 | MS.gene69978:intron |
| TTGTTGACAGTCCTGTCCTT+TGG | 0.407756 | 8.1:+32629468 | MS.gene69978:intron |
| AACTGTTTGTTGTTGTTCAA+TGG | 0.409950 | 8.1:-32631989 | None:intergenic |
| CAGCAGTTTGTCTGCCGCAT+TGG | 0.418700 | 8.1:-32623971 | None:intergenic |
| GCGCTTCTTTCCGGATGATC+AGG | 0.418756 | 8.1:-32632036 | None:intergenic |
| ATGTAGATCCTTCAAGATTC+CGG | 0.429249 | 8.1:+32628942 | MS.gene69978:intron |
| AGAATATGAGGATGTGTAAC+TGG | 0.429823 | 8.1:-32624637 | None:intergenic |
| TGTGTCTGGTTTCATTTGCA+TGG | 0.430249 | 8.1:+32623051 | MS.gene69978:CDS |
| GTGCTGTCACAATGCTTCTT+GGG | 0.431222 | 8.1:+32624687 | MS.gene69978:CDS |
| TTCAAATAACATATTTCTTG+TGG | 0.435052 | 8.1:+32630665 | MS.gene69978:CDS |
| TGAGTAACAAGAGCATATAA+TGG | 0.437137 | 8.1:-32631248 | None:intergenic |
| ATCCTTGGAGGATGACTTCA+AGG | 0.437300 | 8.1:+32629338 | MS.gene69978:CDS |
| TAAATCCGTTCCGCAGAGTC+AGG | 0.442186 | 8.1:-32629187 | None:intergenic |
| TCAAGGTGGTTGTCGGAGTC+AGG | 0.443231 | 8.1:+32629355 | MS.gene69978:CDS |
| CTCCTTGCATTTGTAAGGGG+TGG | 0.449172 | 8.1:-32624091 | None:intergenic |
| TATCAGATTGGTAATCATCT+TGG | 0.458388 | 8.1:-32631864 | None:intergenic |
| GAAAGTCGAGCGTGCGAACC+AGG | 0.460926 | 8.1:+32631749 | MS.gene69978:CDS |
| AGTTTGTCTGCCGCATTGGC+AGG | 0.461254 | 8.1:-32623967 | None:intergenic |
| ATTTGTCAAGCCTGATCATC+CGG | 0.461617 | 8.1:+32632026 | MS.gene69978:CDS |
| AAAATATATCAAAAGATCCT+TGG | 0.464347 | 8.1:+32629323 | MS.gene69978:CDS |
| ATTATATGCTCTTGTTACTC+AGG | 0.468536 | 8.1:+32631250 | MS.gene69978:CDS |
| GTACAATGCTAGTGTGGCTA+TGG | 0.475563 | 8.1:+32623076 | MS.gene69978:CDS |
| AATGCTTCTTGGGAGACTAA+AGG | 0.478573 | 8.1:+32624697 | MS.gene69978:CDS |
| CTTACTATATAGAAGAAGAT+TGG | 0.478608 | 8.1:-32629019 | None:intergenic |
| TACCTTCAACATTTAGGAGA+AGG | 0.491813 | 8.1:-32629508 | None:intergenic |
| ATGATGAAGAGGAACATCAT+AGG | 0.491993 | 8.1:+32624028 | MS.gene69978:CDS |
| AATAGAGTGCACTGTTGAAT+CGG | 0.495992 | 8.1:-32631785 | None:intergenic |
| AACAGAATACTTACAATGAC+AGG | 0.501258 | 8.1:-32629654 | None:intergenic |
| TTTGTCGGACCCTTGAACAA+GGG | 0.507441 | 8.1:-32630178 | None:intergenic |
| TGTGTAAGCCGGAATCTTGA+AGG | 0.507985 | 8.1:-32628950 | None:intergenic |
| CAATGGTTTCTACGATGGCT+TGG | 0.509809 | 8.1:-32631972 | None:intergenic |
| GTGCAGGTCCACCTCTCACC+AGG | 0.511637 | 8.1:+32629282 | MS.gene69978:intron |
| GAGAGACATGCTGTGGTCCA+AGG | 0.518835 | 8.1:+32630150 | MS.gene69978:CDS |
| GATGACTTCAAGGTGGTTGT+CGG | 0.519919 | 8.1:+32629348 | MS.gene69978:CDS |
| AAGGGATTCCCCTTGTTCAA+GGG | 0.522129 | 8.1:+32630169 | MS.gene69978:CDS |
| TTACCTCCTTGCATTTGTAA+GGG | 0.525913 | 8.1:-32624095 | None:intergenic |
| TTGTTCAATGGTTTCTACGA+TGG | 0.528756 | 8.1:-32631977 | None:intergenic |
| AAGAAGCATGGAGTGAAACT+AGG | 0.529232 | 8.1:+32631727 | MS.gene69978:CDS |
| TCAAGCCACATTGTCAAAGA+TGG | 0.535349 | 8.1:+32630113 | MS.gene69978:CDS |
| GAGCCACTTATATCTGTCAA+TGG | 0.535785 | 8.1:+32624608 | MS.gene69978:CDS |
| CTTGAACAAGGGGAATCCCT+TGG | 0.536127 | 8.1:-32630167 | None:intergenic |
| TAACAAGAGCATATAATGGA+AGG | 0.538059 | 8.1:-32631244 | None:intergenic |
| TACCTCCTTGCATTTGTAAG+GGG | 0.540174 | 8.1:-32624094 | None:intergenic |
| AAGTTTGACGTTGGTGATGT+CGG | 0.540688 | 8.1:-32631898 | None:intergenic |
| CCCATGTTGGTGTCTCATCA+AGG | 0.547305 | 8.1:-32623133 | None:intergenic |
| ATGATGTTTCATGTGTAAGC+CGG | 0.550936 | 8.1:-32628961 | None:intergenic |
| ACGAATGATGCCCAAAGGAC+AGG | 0.551759 | 8.1:-32629479 | None:intergenic |
| TATGTAGATACGAGGATGGA+AGG | 0.556059 | 8.1:+32625553 | MS.gene69978:intron |
| TAAATTTACTTCCTGGTGAG+AGG | 0.558314 | 8.1:-32629293 | None:intergenic |
| TCGAGCGTGCGAACCAGGGA+CGG | 0.562166 | 8.1:+32631754 | MS.gene69978:CDS |
| AGATACGAGGATGGAAGGCA+TGG | 0.566527 | 8.1:+32625558 | MS.gene69978:intron |
| ATTTGTCGGACCCTTGAACA+AGG | 0.569217 | 8.1:-32630179 | None:intergenic |
| GCTTCTTCAAGTGAAGGAAG+TGG | 0.571595 | 8.1:+32623098 | MS.gene69978:CDS |
| ATATATCAAAAGATCCTTGG+AGG | 0.572516 | 8.1:+32629326 | MS.gene69978:CDS |
| ATTTACTTCCTGGTGAGAGG+TGG | 0.573609 | 8.1:-32629290 | None:intergenic |
| CTACACCGTTACAAAACTAC+CGG | 0.575503 | 8.1:+32631817 | MS.gene69978:CDS |
| AATAGCCATCTTTGACAATG+TGG | 0.578173 | 8.1:-32630118 | None:intergenic |
| TTGTCGGACCCTTGAACAAG+GGG | 0.578428 | 8.1:-32630177 | None:intergenic |
| AATCCATTGACAGATATAAG+TGG | 0.579163 | 8.1:-32624611 | None:intergenic |
| ATTTGATGATCAAACATCAA+AGG | 0.581307 | 8.1:+32631675 | MS.gene69978:CDS |
| AGAGACATGCTGTGGTCCAA+GGG | 0.583506 | 8.1:+32630151 | MS.gene69978:CDS |
| GGCTGGGAGATAGGCACAAG+AGG | 0.584845 | 8.1:+32623476 | MS.gene69978:CDS |
| CACGGATATGAGTTCGCCAA+TGG | 0.585068 | 8.1:+32625599 | MS.gene69978:CDS |
| CGGGAAGCTCTATATGGACA+TGG | 0.587037 | 8.1:-32623994 | None:intergenic |
| GAGTCAATTCAATATCAGAT+TGG | 0.589879 | 8.1:-32631876 | None:intergenic |
| TTGCAAGCTTGTAATCAGCA+TGG | 0.596652 | 8.1:-32630809 | None:intergenic |
| GAAGGGTCATGTAGCTGCAG+AGG | 0.603722 | 8.1:-32631226 | None:intergenic |
| AACAAGAGCATATAATGGAA+GGG | 0.604802 | 8.1:-32631243 | None:intergenic |
| ATCGTAGCGAGTTGAGTGAC+CGG | 0.608887 | 8.1:-32631836 | None:intergenic |
| CACCTTGAAGTCATCCTCCA+AGG | 0.612962 | 8.1:-32629340 | None:intergenic |
| TGCCACCCCTTACAAATGCA+AGG | 0.613748 | 8.1:+32624089 | MS.gene69978:CDS |
| GCTATGGCTTCTTCAAGTGA+AGG | 0.625236 | 8.1:+32623092 | MS.gene69978:CDS |
| GACTACCTGACTCTGCGGAA+CGG | 0.625975 | 8.1:+32629182 | MS.gene69978:CDS |
| AAAGTCGAGCGTGCGAACCA+GGG | 0.634289 | 8.1:+32631750 | MS.gene69978:CDS |
| ACTGCTAGCAAGTTTGACGT+TGG | 0.635051 | 8.1:-32631907 | None:intergenic |
| ACGAGGATGGAAGGCATGGG+AGG | 0.635904 | 8.1:+32625562 | MS.gene69978:CDS |
| GATACGAGGATGGAAGGCAT+GGG | 0.637824 | 8.1:+32625559 | MS.gene69978:intron |
| TTATGTCGAAGTACAAACAA+AGG | 0.646971 | 8.1:+32623021 | None:intergenic |
| ACCTTGATGAGACACCAACA+TGG | 0.651649 | 8.1:+32623132 | MS.gene69978:CDS |
| AAAATTGAATACCTGGAACA+AGG | 0.654833 | 8.1:-32630250 | None:intergenic |
| ATGAGATGAAGAAGCAACTG+AGG | 0.659339 | 8.1:-32630219 | None:intergenic |
| TCTGCGGAACGGATTTATTG+CGG | 0.660034 | 8.1:+32629193 | MS.gene69978:CDS |
| GAGGCAGAGACATCGTCTCA+CGG | 0.661631 | 8.1:+32625581 | MS.gene69978:CDS |
| ATGGCTGTACAATGCTAGTG+TGG | 0.665019 | 8.1:+32623070 | MS.gene69978:CDS |
| ACACAAGCAACAGCCCATGT+TGG | 0.665589 | 8.1:-32623146 | None:intergenic |
| TACAATGACAGGAATTAAGG+AGG | 0.671511 | 8.1:-32629643 | None:intergenic |
| CCTTGATGAGACACCAACAT+GGG | 0.673354 | 8.1:+32623133 | MS.gene69978:CDS |
| CAGCACTGTATATCACGTGA+AGG | 0.678665 | 8.1:-32624670 | None:intergenic |
| GCTGGGAGATAGGCACAAGA+GGG | 0.683674 | 8.1:+32623477 | MS.gene69978:CDS |
| AAACAACGAATGATGCCCAA+AGG | 0.683770 | 8.1:-32629484 | None:intergenic |
| AATATGCATTCCTGCCAATG+CGG | 0.698892 | 8.1:+32623957 | MS.gene69978:CDS |
| AAGCTGACTACCTGACTCTG+CGG | 0.706617 | 8.1:+32629177 | MS.gene69978:CDS |
| CTTGGAGGATGACTTCAAGG+TGG | 0.722110 | 8.1:+32629341 | MS.gene69978:CDS |
| AATCACAGAGAGACATGCTG+TGG | 0.723027 | 8.1:+32630143 | MS.gene69978:CDS |
| CACCCCTTACAAATGCAAGG+AGG | 0.759926 | 8.1:+32624092 | MS.gene69978:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 32623023 | 32632065 | 32623023 | ID=MS.gene69978 |
| chr8.1 | mRNA | 32623023 | 32632065 | 32623023 | ID=MS.gene69978.t1;Parent=MS.gene69978 |
| chr8.1 | exon | 32623023 | 32623229 | 32623023 | ID=MS.gene69978.t1.exon1;Parent=MS.gene69978.t1 |
| chr8.1 | CDS | 32623023 | 32623229 | 32623023 | ID=cds.MS.gene69978.t1;Parent=MS.gene69978.t1 |
| chr8.1 | exon | 32623475 | 32623532 | 32623475 | ID=MS.gene69978.t1.exon2;Parent=MS.gene69978.t1 |
| chr8.1 | CDS | 32623475 | 32623532 | 32623475 | ID=cds.MS.gene69978.t1;Parent=MS.gene69978.t1 |
| chr8.1 | exon | 32623896 | 32624113 | 32623896 | ID=MS.gene69978.t1.exon3;Parent=MS.gene69978.t1 |
| chr8.1 | CDS | 32623896 | 32624113 | 32623896 | ID=cds.MS.gene69978.t1;Parent=MS.gene69978.t1 |
| chr8.1 | exon | 32624602 | 32624718 | 32624602 | ID=MS.gene69978.t1.exon4;Parent=MS.gene69978.t1 |
| chr8.1 | CDS | 32624602 | 32624718 | 32624602 | ID=cds.MS.gene69978.t1;Parent=MS.gene69978.t1 |
| chr8.1 | exon | 32625560 | 32625620 | 32625560 | ID=MS.gene69978.t1.exon5;Parent=MS.gene69978.t1 |
| chr8.1 | CDS | 32625560 | 32625620 | 32625560 | ID=cds.MS.gene69978.t1;Parent=MS.gene69978.t1 |
| chr8.1 | exon | 32628948 | 32629036 | 32628948 | ID=MS.gene69978.t1.exon6;Parent=MS.gene69978.t1 |
| chr8.1 | CDS | 32628948 | 32629036 | 32628948 | ID=cds.MS.gene69978.t1;Parent=MS.gene69978.t1 |
| chr8.1 | exon | 32629140 | 32629214 | 32629140 | ID=MS.gene69978.t1.exon7;Parent=MS.gene69978.t1 |
| chr8.1 | CDS | 32629140 | 32629214 | 32629140 | ID=cds.MS.gene69978.t1;Parent=MS.gene69978.t1 |
| chr8.1 | exon | 32629288 | 32629376 | 32629288 | ID=MS.gene69978.t1.exon8;Parent=MS.gene69978.t1 |
| chr8.1 | CDS | 32629288 | 32629376 | 32629288 | ID=cds.MS.gene69978.t1;Parent=MS.gene69978.t1 |
| chr8.1 | exon | 32629478 | 32629527 | 32629478 | ID=MS.gene69978.t1.exon9;Parent=MS.gene69978.t1 |
| chr8.1 | CDS | 32629478 | 32629527 | 32629478 | ID=cds.MS.gene69978.t1;Parent=MS.gene69978.t1 |
| chr8.1 | exon | 32629622 | 32629662 | 32629622 | ID=MS.gene69978.t1.exon10;Parent=MS.gene69978.t1 |
| chr8.1 | CDS | 32629622 | 32629662 | 32629622 | ID=cds.MS.gene69978.t1;Parent=MS.gene69978.t1 |
| chr8.1 | exon | 32630087 | 32630260 | 32630087 | ID=MS.gene69978.t1.exon11;Parent=MS.gene69978.t1 |
| chr8.1 | CDS | 32630087 | 32630260 | 32630087 | ID=cds.MS.gene69978.t1;Parent=MS.gene69978.t1 |
| chr8.1 | exon | 32630658 | 32630693 | 32630658 | ID=MS.gene69978.t1.exon12;Parent=MS.gene69978.t1 |
| chr8.1 | CDS | 32630658 | 32630693 | 32630658 | ID=cds.MS.gene69978.t1;Parent=MS.gene69978.t1 |
| chr8.1 | exon | 32630783 | 32630850 | 32630783 | ID=MS.gene69978.t1.exon13;Parent=MS.gene69978.t1 |
| chr8.1 | CDS | 32630783 | 32630850 | 32630783 | ID=cds.MS.gene69978.t1;Parent=MS.gene69978.t1 |
| chr8.1 | exon | 32631211 | 32631271 | 32631211 | ID=MS.gene69978.t1.exon14;Parent=MS.gene69978.t1 |
| chr8.1 | CDS | 32631211 | 32631271 | 32631211 | ID=cds.MS.gene69978.t1;Parent=MS.gene69978.t1 |
| chr8.1 | exon | 32631649 | 32632065 | 32631649 | ID=MS.gene69978.t1.exon15;Parent=MS.gene69978.t1 |
| chr8.1 | CDS | 32631649 | 32632065 | 32631649 | ID=cds.MS.gene69978.t1;Parent=MS.gene69978.t1 |
| Gene Sequence |
| Protein sequence |
