AlfalfaGEDB | MS.gene70327

M. sativa cultivar XinJiangDaYe / MS.gene70327

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70327.t1	RHN42992.1	91	199	12	2	1	198	1	194	2.60E-89	338.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70327.t1	Q9SJ02	55.4	195	81	3	1	192	1	192	9.1e-43	174.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70327.t1	A0A396GPG0	91.0	199	12	2	1	198	1	194	1.9e-89	338.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050338	MS.gene70327	-0.813499	2.48E-51	-1.69E-46
MS.gene052100	MS.gene70327	0.85179	7.06E-61	-1.69E-46
MS.gene054941	MS.gene70327	-0.831596	1.53E-55	-1.69E-46
MS.gene05589	MS.gene70327	0.809184	2.14E-50	-1.69E-46
MS.gene056266	MS.gene70327	0.803243	3.83E-49	-1.69E-46
MS.gene056283	MS.gene70327	0.832117	1.14E-55	-1.69E-46
MS.gene056387	MS.gene70327	0.847346	1.23E-59	-1.69E-46
MS.gene05652	MS.gene70327	-0.816578	5.14E-52	-1.69E-46
MS.gene056618	MS.gene70327	-0.811049	8.50E-51	-1.69E-46
MS.gene058448	MS.gene70327	0.806026	1.00E-49	-1.69E-46
MS.gene05926	MS.gene70327	-0.813699	2.24E-51	-1.69E-46
MS.gene059717	MS.gene70327	0.883884	3.07E-71	-1.69E-46
MS.gene06000	MS.gene70327	0.806465	8.12E-50	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene70327.t1	AT2G21970	55.385	195	81	3	1	192	1	192	3.04e-62	192

Find 62 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ATGTTTGTGTTCGCGCCTTC+TGG	0.200953	4.1:+9552129	None:intergenic
TTCTTTACGGGTCTTGATTA+TGG	0.222006	4.1:+9552050	None:intergenic
GCCTTCTGGTTCACTCGGTT+TGG	0.276085	4.1:+9552143	None:intergenic
CTTGCATGTCTTCTTCTTTA+CGG	0.282945	4.1:+9552037	None:intergenic
TTGCATGTCTTCTTCTTTAC+GGG	0.300636	4.1:+9552038	None:intergenic
CTTAGAACTCTCGATATATT+CGG	0.302152	4.1:+9551978	None:intergenic
CCTTCTGGTTCACTCGGTTT+GGG	0.307109	4.1:+9552144	None:intergenic
TTGTTTGGGTGCGGTTACTA+TGG	0.311981	4.1:-9551675	MS.gene70327:CDS
GAATTCGGTGTTCAGGAAAA+TGG	0.321728	4.1:-9551729	MS.gene70327:CDS
GAGGCGGGTGGGATTTGTTT+GGG	0.354432	4.1:-9551689	MS.gene70327:CDS
GGAGGCGGGTGGGATTTGTT+TGG	0.366265	4.1:-9551690	MS.gene70327:CDS
TGGTTCACTCGGTTTGGGTT+TGG	0.379173	4.1:+9552149	None:intergenic
GAACGAGACGATGATACTTT+TGG	0.405757	4.1:+9552210	None:intergenic
GGAGTTGGTGACGGGGAATT+CGG	0.411121	4.1:-9551744	MS.gene70327:CDS
TTCAGGAAAATGGATATTGC+AGG	0.423134	4.1:-9551719	MS.gene70327:CDS
TGACGGGGAATTCGGTGTTC+AGG	0.428337	4.1:-9551736	MS.gene70327:CDS
ATCAATTAGCGAATCAATGA+AGG	0.432023	4.1:+9551583	None:intergenic
CGGAGATGATTTCGAAATCC+TGG	0.437279	4.1:+9551950	None:intergenic
CTATGGCGGCAATGTTTGCT+TGG	0.440446	4.1:-9551658	MS.gene70327:CDS
GTGACGGTGGAGTTGGTGAC+GGG	0.448839	4.1:-9551752	MS.gene70327:CDS
CTAATTGATCAAATCGTTGA+CGG	0.458559	4.1:-9551569	MS.gene70327:CDS
GTCTTGATTATGGCGCCGAC+TGG	0.478139	4.1:+9552060	None:intergenic
CGTTCCCGCCCCAGAAAACC+TGG	0.481238	4.1:-9552192	MS.gene70327:CDS
TGTTACCATGGCGAGGCGGC+CGG	0.483455	4.1:+9551930	None:intergenic
CCGCCCCAGAAAACCTGGTC+CGG	0.484254	4.1:-9552187	MS.gene70327:CDS
TTGGTGACGGAAAATTGAAC+CGG	0.499068	4.1:+9552168	None:intergenic
GATTATGGCGCCGACTGGAT+CGG	0.505400	4.1:+9552065	None:intergenic
AATGGATATTGCAGGGATAA+CGG	0.508574	4.1:-9551711	MS.gene70327:CDS
TTCGCGCCTTCTGGTTCACT+CGG	0.509001	4.1:+9552138	None:intergenic
ACTCGGTTTGGGTTTGGTGA+CGG	0.510065	4.1:+9552155	None:intergenic
TTCGGAAAGAGTGGCGAAGA+AGG	0.517357	4.1:+9551996	None:intergenic
TATTGCAGGGATAACGGAGG+CGG	0.525078	4.1:-9551705	MS.gene70327:CDS
ACGGAAAATTGAACCGGACC+AGG	0.525085	4.1:+9552174	None:intergenic
GCAGATGGTGTTTGCAGCGA+CGG	0.525390	4.1:-9551774	MS.gene70327:intron
TTTGGGTGCGGTTACTATGG+CGG	0.548728	4.1:-9551672	MS.gene70327:CDS
CTATGCACTCTCAGATCATA+CGG	0.549836	4.1:-9552090	MS.gene70327:CDS
GGGTGGGATTTGTTTGGGTG+CGG	0.551031	4.1:-9551684	MS.gene70327:CDS
CAGGGATAACGGAGGCGGGT+GGG	0.551178	4.1:-9551700	MS.gene70327:CDS
GCAGGGATAACGGAGGCGGG+TGG	0.556957	4.1:-9551701	MS.gene70327:CDS
CGAGAGTTCTAAGAAAAGCC+AGG	0.559263	4.1:-9551968	MS.gene70327:CDS
GGTGACGGTGGAGTTGGTGA+CGG	0.559321	4.1:-9551753	MS.gene70327:CDS
GTGTGACTAACTGCGAGCTC+CGG	0.567817	4.1:-9552236	MS.gene70327:CDS
ATTGCAGGGATAACGGAGGC+GGG	0.570947	4.1:-9551704	MS.gene70327:CDS
CATCTCCGGCCGCCTCGCCA+TGG	0.570988	4.1:-9551935	MS.gene70327:intron
TGACGGTGGAGTTGGTGACG+GGG	0.573283	4.1:-9551751	MS.gene70327:CDS
CAGGATTTCGAAATCATCTC+CGG	0.574628	4.1:-9551949	MS.gene70327:CDS
AGCGACGGTGACGGTGGAGT+TGG	0.587633	4.1:-9551759	MS.gene70327:CDS
CTCGATATATTCGGAAAGAG+TGG	0.596447	4.1:+9551987	None:intergenic
TGTGAAGATTCGATCCACTC+TGG	0.602920	4.1:+9551619	None:intergenic
GAAGAAGGAGGACACGTCAT+CGG	0.616933	4.1:+9552011	None:intergenic
GGAAAGAGTGGCGAAGAAGG+AGG	0.623174	4.1:+9551999	None:intergenic
GGTGTTTGCAGCGACGGTGA+CGG	0.625726	4.1:-9551768	MS.gene70327:CDS
CTCAAGTGCTCGTACCAGAG+TGG	0.631041	4.1:-9551633	MS.gene70327:CDS
TTGTTCTATGATGATAGCGA+GGG	0.632266	4.1:-9551545	MS.gene70327:CDS
TTTGTTCTATGATGATAGCG+AGG	0.632796	4.1:-9551546	MS.gene70327:CDS
TCAGGAAAATGGATATTGCA+GGG	0.643208	4.1:-9551718	MS.gene70327:CDS
CAAACATCGTTCTACAACCG+CGG	0.645183	4.1:-9552113	MS.gene70327:CDS
GTTTGCAGCGACGGTGACGG+TGG	0.675734	4.1:-9551765	MS.gene70327:CDS
CCCAAACCGAGTGAACCAGA+AGG	0.690718	4.1:-9552144	MS.gene70327:CDS
GGATATTGCAGGGATAACGG+AGG	0.721943	4.1:-9551708	MS.gene70327:CDS
GATCTGAGAGTGCATAGCCG+CGG	0.723172	4.1:+9552096	None:intergenic
TCATACGGTTCCGATCCAGT+CGG	0.759723	4.1:-9552075	MS.gene70327:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CATTATTCTCAGTTTATTAT+TGG	-	chr4.1:9551920-9551939	MS.gene70327:intron	20.0%
!!	TTCCTCTAATATAGTATATA+TGG	+	chr4.1:9551963-9551982	None:intergenic	20.0%
!	AATATAGTATATATGGACAC+AGG	+	chr4.1:9551956-9551975	None:intergenic	25.0%
!	GTCCATATATACTATATTAG+AGG	-	chr4.1:9551958-9551977	MS.gene70327:CDS	25.0%
!!!	ATTGTTGCTTTTTATTGATG+TGG	-	chr4.1:9551986-9552005	MS.gene70327:CDS	25.0%
	ATCAATTAGCGAATCAATGA+AGG	+	chr4.1:9552207-9552226	None:intergenic	30.0%
	CTAATTGATCAAATCGTTGA+CGG	-	chr4.1:9552218-9552237	MS.gene70327:CDS	30.0%
	CTTAGAACTCTCGATATATT+CGG	+	chr4.1:9551812-9551831	None:intergenic	30.0%
	AATGGATATTGCAGGGATAA+CGG	-	chr4.1:9552076-9552095	MS.gene70327:CDS	35.0%
	CTTGCATGTCTTCTTCTTTA+CGG	+	chr4.1:9551753-9551772	None:intergenic	35.0%
	GATCACATGTGTTAGTGTTA+GGG	+	chr4.1:9551886-9551905	None:intergenic	35.0%
	TCAGGAAAATGGATATTGCA+GGG	-	chr4.1:9552069-9552088	MS.gene70327:CDS	35.0%
	TGATCACATGTGTTAGTGTT+AGG	+	chr4.1:9551887-9551906	None:intergenic	35.0%
	TTCAGGAAAATGGATATTGC+AGG	-	chr4.1:9552068-9552087	MS.gene70327:CDS	35.0%
	TTCTTTACGGGTCTTGATTA+TGG	+	chr4.1:9551740-9551759	None:intergenic	35.0%
	TTGCATGTCTTCTTCTTTAC+GGG	+	chr4.1:9551752-9551771	None:intergenic	35.0%
!	TTGTTCTATGATGATAGCGA+GGG	-	chr4.1:9552242-9552261	MS.gene70327:CDS	35.0%
!	TTTGTTCTATGATGATAGCG+AGG	-	chr4.1:9552241-9552260	MS.gene70327:CDS	35.0%
!!!	GTTGCTTTTTATTGATGTGG+TGG	-	chr4.1:9551989-9552008	MS.gene70327:CDS	35.0%
	CAGGATTTCGAAATCATCTC+CGG	-	chr4.1:9551838-9551857	MS.gene70327:intron	40.0%
	CTATGCACTCTCAGATCATA+CGG	-	chr4.1:9551697-9551716	MS.gene70327:CDS	40.0%
	CTCGATATATTCGGAAAGAG+TGG	+	chr4.1:9551803-9551822	None:intergenic	40.0%
!	GAACGAGACGATGATACTTT+TGG	+	chr4.1:9551580-9551599	None:intergenic	40.0%
!!	GAATTCGGTGTTCAGGAAAA+TGG	-	chr4.1:9552058-9552077	MS.gene70327:CDS	40.0%
!!	TTGGTGACGGAAAATTGAAC+CGG	+	chr4.1:9551622-9551641	None:intergenic	40.0%
!!!	GTGTTAGGGTTTTGTTACCA+TGG	+	chr4.1:9551872-9551891	None:intergenic	40.0%
	CAAACATCGTTCTACAACCG+CGG	-	chr4.1:9551674-9551693	MS.gene70327:CDS	45.0%
	CGGAGATGATTTCGAAATCC+TGG	+	chr4.1:9551840-9551859	None:intergenic	45.0%
	TGTGAAGATTCGATCCACTC+TGG	+	chr4.1:9552171-9552190	None:intergenic	45.0%
	TTGTTTGGGTGCGGTTACTA+TGG	-	chr4.1:9552112-9552131	MS.gene70327:CDS	45.0%
!	CGAGACGATGATACTTTTGG+TGG	+	chr4.1:9551577-9551596	None:intergenic	45.0%
!	TATTGATGTGGTGGTGCAGA+TGG	-	chr4.1:9551998-9552017	MS.gene70327:CDS	45.0%
!!	ACGATGATACTTTTGGTGGC+CGG	+	chr4.1:9551573-9551592	None:intergenic	45.0%
!!	CGAGAGTTCTAAGAAAAGCC+AGG	-	chr4.1:9551819-9551838	MS.gene70327:intron	45.0%
	ACGGAAAATTGAACCGGACC+AGG	+	chr4.1:9551616-9551635	None:intergenic	50.0%
	ATGTTTGTGTTCGCGCCTTC+TGG	+	chr4.1:9551661-9551680	None:intergenic	50.0%
	CTATGGCGGCAATGTTTGCT+TGG	-	chr4.1:9552129-9552148	MS.gene70327:CDS	50.0%
	GAAGAAGGAGGACACGTCAT+CGG	+	chr4.1:9551779-9551798	None:intergenic	50.0%
	TCATACGGTTCCGATCCAGT+CGG	-	chr4.1:9551712-9551731	MS.gene70327:CDS	50.0%
	TTCGGAAAGAGTGGCGAAGA+AGG	+	chr4.1:9551794-9551813	None:intergenic	50.0%
	TTTGGGTGCGGTTACTATGG+CGG	-	chr4.1:9552115-9552134	MS.gene70327:CDS	50.0%
!	GGATATTGCAGGGATAACGG+AGG	-	chr4.1:9552079-9552098	MS.gene70327:CDS	50.0%
!	TGAACCGGACCAGGTTTTCT+GGG	+	chr4.1:9551607-9551626	None:intergenic	50.0%
!	TTGAACCGGACCAGGTTTTC+TGG	+	chr4.1:9551608-9551627	None:intergenic	50.0%
!!	ACTCGGTTTGGGTTTGGTGA+CGG	+	chr4.1:9551635-9551654	None:intergenic	50.0%
!!	CCTTCTGGTTCACTCGGTTT+GGG	+	chr4.1:9551646-9551665	None:intergenic	50.0%
!!	GTTTTGTTACCATGGCGAGG+CGG	+	chr4.1:9551864-9551883	None:intergenic	50.0%
!!	TATTGCAGGGATAACGGAGG+CGG	-	chr4.1:9552082-9552101	MS.gene70327:CDS	50.0%
!!	TGGTTCACTCGGTTTGGGTT+TGG	+	chr4.1:9551641-9551660	None:intergenic	50.0%
!!!	AGGGTTTTGTTACCATGGCG+AGG	+	chr4.1:9551867-9551886	None:intergenic	50.0%
	CCCAAACCGAGTGAACCAGA+AGG	-	chr4.1:9551643-9551662	MS.gene70327:CDS	55.0%
	CTCAAGTGCTCGTACCAGAG+TGG	-	chr4.1:9552154-9552173	MS.gene70327:CDS	55.0%
	GCAGATGGTGTTTGCAGCGA+CGG	-	chr4.1:9552013-9552032	MS.gene70327:CDS	55.0%
	GGAAAGAGTGGCGAAGAAGG+AGG	+	chr4.1:9551791-9551810	None:intergenic	55.0%
	GTGTGACTAACTGCGAGCTC+CGG	-	chr4.1:9551551-9551570	MS.gene70327:CDS	55.0%
!	GAACCGGACCAGGTTTTCTG+GGG	+	chr4.1:9551606-9551625	None:intergenic	55.0%
!	GAGGCGGGTGGGATTTGTTT+GGG	-	chr4.1:9552098-9552117	MS.gene70327:CDS	55.0%
!	GATCTGAGAGTGCATAGCCG+CGG	+	chr4.1:9551694-9551713	None:intergenic	55.0%
!	GGGTGGGATTTGTTTGGGTG+CGG	-	chr4.1:9552103-9552122	MS.gene70327:CDS	55.0%
!!	ATTGCAGGGATAACGGAGGC+GGG	-	chr4.1:9552083-9552102	MS.gene70327:CDS	55.0%
!!	GATTATGGCGCCGACTGGAT+CGG	+	chr4.1:9551725-9551744	None:intergenic	55.0%
!!	GCCTTCTGGTTCACTCGGTT+TGG	+	chr4.1:9551647-9551666	None:intergenic	55.0%
!!	GGAGTTGGTGACGGGGAATT+CGG	-	chr4.1:9552043-9552062	MS.gene70327:CDS	55.0%
!!	GTCTTGATTATGGCGCCGAC+TGG	+	chr4.1:9551730-9551749	None:intergenic	55.0%
!!	TGACGGGGAATTCGGTGTTC+AGG	-	chr4.1:9552051-9552070	MS.gene70327:CDS	55.0%
!!	TTCGCGCCTTCTGGTTCACT+CGG	+	chr4.1:9551652-9551671	None:intergenic	55.0%
	GGTGTTTGCAGCGACGGTGA+CGG	-	chr4.1:9552019-9552038	MS.gene70327:CDS	60.0%
!	GGAGGCGGGTGGGATTTGTT+TGG	-	chr4.1:9552097-9552116	MS.gene70327:CDS	60.0%
!!	GGTGACGGTGGAGTTGGTGA+CGG	-	chr4.1:9552034-9552053	MS.gene70327:CDS	60.0%
!!	GTGACGGTGGAGTTGGTGAC+GGG	-	chr4.1:9552035-9552054	MS.gene70327:CDS	60.0%
!!	TGACGGTGGAGTTGGTGACG+GGG	-	chr4.1:9552036-9552055	MS.gene70327:CDS	60.0%
	CCGCCCCAGAAAACCTGGTC+CGG	-	chr4.1:9551600-9551619	MS.gene70327:CDS	65.0%
	CGTTCCCGCCCCAGAAAACC+TGG	-	chr4.1:9551595-9551614	MS.gene70327:CDS	65.0%
	TGTTACCATGGCGAGGCGGC+CGG	+	chr4.1:9551860-9551879	None:intergenic	65.0%
!	AGCGACGGTGACGGTGGAGT+TGG	-	chr4.1:9552028-9552047	MS.gene70327:CDS	65.0%
!	GTTTGCAGCGACGGTGACGG+TGG	-	chr4.1:9552022-9552041	MS.gene70327:CDS	65.0%
!!	CAGGGATAACGGAGGCGGGT+GGG	-	chr4.1:9552087-9552106	MS.gene70327:CDS	65.0%
!!	CCGGACCAGGTTTTCTGGGG+CGG	+	chr4.1:9551603-9551622	None:intergenic	65.0%
!!!	CGGACCAGGTTTTCTGGGGC+GGG	+	chr4.1:9551602-9551621	None:intergenic	65.0%
!!	GCAGGGATAACGGAGGCGGG+TGG	-	chr4.1:9552086-9552105	MS.gene70327:CDS	70.0%
	CATCTCCGGCCGCCTCGCCA+TGG	-	chr4.1:9551852-9551871	MS.gene70327:intron	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	9551535	9552274	9551535	ID=MS.gene70327
chr4.1	mRNA	9551535	9552274	9551535	ID=MS.gene70327.t1;Parent=MS.gene70327
chr4.1	exon	9551936	9552274	9551936	ID=MS.gene70327.t1.exon1;Parent=MS.gene70327.t1
chr4.1	CDS	9551936	9552274	9551936	ID=cds.MS.gene70327.t1;Parent=MS.gene70327.t1
chr4.1	exon	9551535	9551792	9551535	ID=MS.gene70327.t1.exon2;Parent=MS.gene70327.t1
chr4.1	CDS	9551535	9551792	9551535	ID=cds.MS.gene70327.t1;Parent=MS.gene70327.t1

Gene Sequence

>MS.gene70327

ATGGCATCTGCAGCGCGTGTGACTAACTGCGAGCTCCGGCCACCAAAAGTATCATCGTCTCGTTCCCGCCCCAGAAAACCTGGTCCGGTTCAATTTTCCGTCACCAAACCCAAACCGAGTGAACCAGAAGGCGCGAACACAAACATCGTTCTACAACCGCGGCTATGCACTCTCAGATCATACGGTTCCGATCCAGTCGGCGCCATAATCAAGACCCGTAAAGAAGAAGACATGCAAGTTACCGATGACGTGTCCTCCTTCTTCGCCACTCTTTCCGAATATATCGAGAGTTCTAAGAAAAGCCAGGATTTCGAAATCATCTCCGGCCGCCTCGCCATGGTAACAAAACCCTAACACTAACACATGTGATCACATTGAATTATATCATTATTCTCAGTTTATTATTGGTGTCAACGTGCCTGTGTCCATATATACTATATTAGAGGAACTAATTGTTGCTTTTTATTGATGTGGTGGTGCAGATGGTGTTTGCAGCGACGGTGACGGTGGAGTTGGTGACGGGGAATTCGGTGTTCAGGAAAATGGATATTGCAGGGATAACGGAGGCGGGTGGGATTTGTTTGGGTGCGGTTACTATGGCGGCAATGTTTGCTTGGTTCTCAAGTGCTCGTACCAGAGTGGATCGAATCTTCACAGTCAGCTACAACTCCTTCATTGATTCGCTAATTGATCAAATCGTTGACGGTTTGTTCTATGATGATAGCGAGGGTGAACTCTGA

Protein sequence

>MS.gene70327.t1

MASAARVTNCELRPPKVSSSRSRPRKPGPVQFSVTKPKPSEPEGANTNIVLQPRLCTLRSYGSDPVGAIIKTRKEEDMQVTDDVSSFFATLSEYIESSKKSQDFEIISGRLAMMVFAATVTVELVTGNSVFRKMDIAGITEAGGICLGAVTMAAMFAWFSSARTRVDRIFTVSYNSFIDSLIDQIVDGLFYDDSEGEL