Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70367.t1 | XP_013457187.1 | 96.9 | 1029 | 32 | 0 | 1 | 1029 | 1 | 1029 | 0.00E+00 | 1960.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70367.t1 | Q6NPP4 | 47.4 | 1084 | 481 | 20 | 1 | 1029 | 1 | 1050 | 1.4e-240 | 834.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70367.t1 | A0A072UQ84 | 96.9 | 1029 | 32 | 0 | 1 | 1029 | 1 | 1029 | 0.0e+00 | 1960.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene70367.t1 | TF | CAMTA |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050961 | MS.gene70367 | 0.806158 | 9.42E-50 | -1.69E-46 |
| MS.gene058095 | MS.gene70367 | 0.833433 | 5.35E-56 | -1.69E-46 |
| MS.gene060522 | MS.gene70367 | 0.825613 | 4.26E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene01170 | MS.gene70367 | PPI |
| MS.gene28071 | MS.gene70367 | PPI |
| MS.gene047423 | MS.gene70367 | PPI |
| MS.gene70367 | MS.gene028443 | PPI |
| MS.gene030815 | MS.gene70367 | PPI |
| MS.gene061784 | MS.gene70367 | PPI |
| MS.gene35018 | MS.gene70367 | PPI |
| MS.gene002202 | MS.gene70367 | PPI |
| MS.gene90181 | MS.gene70367 | PPI |
| MS.gene57296 | MS.gene70367 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70367.t1 | MTR_4g094215 | 97.862 | 1029 | 22 | 0 | 1 | 1029 | 1 | 1029 | 0.0 | 2101 |
| MS.gene70367.t1 | MTR_4g094215 | 97.767 | 1030 | 22 | 1 | 1 | 1029 | 1 | 1030 | 0.0 | 2097 |
| MS.gene70367.t1 | MTR_4g094215 | 97.740 | 885 | 20 | 0 | 1 | 885 | 1 | 885 | 0.0 | 1803 |
| MS.gene70367.t1 | MTR_2g008840 | 62.593 | 1080 | 313 | 12 | 1 | 1029 | 1 | 1040 | 0.0 | 1307 |
| MS.gene70367.t1 | MTR_2g008840 | 62.500 | 1040 | 299 | 12 | 41 | 1029 | 1 | 1000 | 0.0 | 1255 |
| MS.gene70367.t1 | MTR_2g008840 | 60.791 | 936 | 278 | 10 | 1 | 885 | 1 | 898 | 0.0 | 1083 |
| MS.gene70367.t1 | MTR_8g090205 | 40.302 | 1124 | 515 | 28 | 12 | 1027 | 8 | 1083 | 0.0 | 704 |
| MS.gene70367.t1 | MTR_8g090205 | 40.284 | 1127 | 517 | 28 | 9 | 1027 | 7 | 1085 | 0.0 | 704 |
| MS.gene70367.t1 | MTR_8g090205 | 40.439 | 1093 | 497 | 26 | 9 | 994 | 7 | 1052 | 0.0 | 691 |
| MS.gene70367.t1 | MTR_8g090205 | 40.459 | 1090 | 495 | 26 | 12 | 994 | 8 | 1050 | 0.0 | 691 |
| MS.gene70367.t1 | MTR_8g090205 | 51.042 | 672 | 281 | 13 | 382 | 1027 | 277 | 926 | 0.0 | 632 |
| MS.gene70367.t1 | MTR_8g090205 | 51.881 | 638 | 261 | 11 | 382 | 994 | 277 | 893 | 0.0 | 620 |
| MS.gene70367.t1 | MTR_8g090205 | 37.563 | 985 | 461 | 26 | 9 | 886 | 7 | 944 | 6.81e-177 | 541 |
| MS.gene70367.t1 | MTR_8g080800 | 38.486 | 621 | 310 | 17 | 426 | 1023 | 389 | 960 | 4.95e-119 | 389 |
| MS.gene70367.t1 | MTR_8g080800 | 50.641 | 156 | 70 | 1 | 14 | 169 | 8 | 156 | 1.02e-42 | 169 |
| MS.gene70367.t1 | MTR_3g085050 | 39.261 | 568 | 305 | 13 | 426 | 984 | 375 | 911 | 1.81e-100 | 337 |
| MS.gene70367.t1 | MTR_3g085050 | 47.445 | 137 | 72 | 0 | 10 | 146 | 4 | 140 | 7.84e-37 | 150 |
| MS.gene70367.t1 | MTR_3g085050 | 39.261 | 568 | 305 | 13 | 426 | 984 | 375 | 911 | 4.78e-100 | 337 |
| MS.gene70367.t1 | MTR_3g085050 | 47.445 | 137 | 72 | 0 | 10 | 146 | 4 | 140 | 8.79e-37 | 150 |
| MS.gene70367.t1 | MTR_2g034650 | 32.432 | 629 | 350 | 18 | 384 | 981 | 301 | 885 | 2.97e-79 | 278 |
| MS.gene70367.t1 | MTR_2g034650 | 55.645 | 124 | 55 | 0 | 13 | 136 | 24 | 147 | 2.96e-37 | 152 |
| MS.gene70367.t1 | MTR_4g121840 | 32.039 | 618 | 366 | 15 | 384 | 981 | 284 | 867 | 4.71e-77 | 272 |
| MS.gene70367.t1 | MTR_4g121840 | 60.241 | 83 | 33 | 0 | 54 | 136 | 36 | 118 | 1.19e-19 | 95.5 |
| MS.gene70367.t1 | MTR_4g121840 | 32.039 | 618 | 366 | 15 | 384 | 981 | 314 | 897 | 5.09e-77 | 272 |
| MS.gene70367.t1 | MTR_4g121840 | 54.839 | 124 | 56 | 0 | 13 | 136 | 25 | 148 | 1.96e-30 | 130 |
| MS.gene70367.t1 | MTR_8g090200 | 27.679 | 448 | 255 | 13 | 115 | 537 | 10 | 413 | 2.35e-37 | 146 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene70367.t1 | AT5G64220 | 50.282 | 1064 | 440 | 20 | 1 | 1010 | 1 | 1029 | 0.0 | 979 |
| MS.gene70367.t1 | AT5G64220 | 50.282 | 1064 | 440 | 20 | 1 | 1010 | 1 | 1029 | 0.0 | 979 |
| MS.gene70367.t1 | AT5G09410 | 51.243 | 1046 | 421 | 26 | 11 | 1024 | 14 | 1002 | 0.0 | 966 |
| MS.gene70367.t1 | AT5G09410 | 50.382 | 1046 | 412 | 26 | 11 | 1024 | 14 | 984 | 0.0 | 945 |
| MS.gene70367.t1 | AT5G09410 | 49.248 | 1064 | 415 | 27 | 11 | 1024 | 73 | 1061 | 0.0 | 911 |
| MS.gene70367.t1 | AT5G09410 | 48.986 | 937 | 389 | 26 | 120 | 1024 | 2 | 881 | 0.0 | 795 |
| MS.gene70367.t1 | AT2G22300 | 42.747 | 1034 | 483 | 21 | 13 | 993 | 13 | 990 | 0.0 | 748 |
| MS.gene70367.t1 | AT2G22300 | 42.747 | 1034 | 483 | 21 | 13 | 993 | 13 | 990 | 0.0 | 748 |
| MS.gene70367.t1 | AT2G22300 | 42.111 | 995 | 453 | 25 | 59 | 993 | 1 | 932 | 0.0 | 686 |
| MS.gene70367.t1 | AT1G67310 | 37.520 | 621 | 332 | 15 | 420 | 1015 | 419 | 1008 | 3.82e-101 | 342 |
| MS.gene70367.t1 | AT1G67310 | 48.000 | 125 | 65 | 0 | 11 | 135 | 34 | 158 | 2.37e-35 | 146 |
| MS.gene70367.t1 | AT3G16940 | 31.475 | 556 | 343 | 10 | 436 | 981 | 274 | 801 | 1.89e-73 | 260 |
| MS.gene70367.t1 | AT3G16940 | 53.906 | 128 | 59 | 0 | 21 | 148 | 4 | 131 | 7.01e-36 | 147 |
| MS.gene70367.t1 | AT3G16940 | 31.475 | 556 | 343 | 10 | 436 | 981 | 301 | 828 | 3.10e-73 | 260 |
| MS.gene70367.t1 | AT3G16940 | 52.206 | 136 | 65 | 0 | 13 | 148 | 23 | 158 | 1.12e-36 | 150 |
| MS.gene70367.t1 | AT3G16940 | 29.847 | 459 | 292 | 9 | 436 | 884 | 301 | 739 | 5.19e-48 | 184 |
| MS.gene70367.t1 | AT3G16940 | 52.206 | 136 | 65 | 0 | 13 | 148 | 23 | 158 | 6.56e-37 | 150 |
Find 266 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAACCTGAAATTCCTTTC+TGG | 0.183571 | 4.3:-20318135 | None:intergenic |
| ACCATACAGGTGTTGATTAT+TGG | 0.185292 | 4.3:+20316875 | MS.gene70367:intron |
| CCATGGACTTAACCCTTAAA+AGG | 0.206382 | 4.3:-20319042 | None:intergenic |
| GGAAGGCTGGTTGGTTGGTT+GGG | 0.230610 | 4.3:-20318950 | None:intergenic |
| CTGTATTGGGCCCGAGCTTC+TGG | 0.238171 | 4.3:-20319073 | None:intergenic |
| GTCTCCATGGGTGCAGCTGC+TGG | 0.249458 | 4.3:+20318043 | MS.gene70367:CDS |
| TGATGCACAGACTGCTCTTT+TGG | 0.251171 | 4.3:-20316250 | None:intergenic |
| AGGTTGACAGCTTCTCTAAA+TGG | 0.265479 | 4.3:+20316323 | MS.gene70367:CDS |
| AATAGGTCAGGATCAATTTC+TGG | 0.268880 | 4.3:-20316211 | None:intergenic |
| TTGCTGCTCTTGGTTATGAT+TGG | 0.275206 | 4.3:+20317461 | MS.gene70367:CDS |
| TTATGTGAAGATGCTGATTC+AGG | 0.276888 | 4.3:+20314126 | MS.gene70367:CDS |
| GAAGAGGATGATGAGGAGTT+TGG | 0.278551 | 4.3:+20318421 | MS.gene70367:CDS |
| GACTGAGTAAAGCTCCATTT+TGG | 0.278754 | 4.3:-20316115 | None:intergenic |
| TAACATGCGTTCAAGAAATA+TGG | 0.284948 | 4.3:+20314071 | MS.gene70367:CDS |
| ACCTAATCGAAGAGCCATTT+CGG | 0.289822 | 4.3:-20310281 | None:intergenic |
| ATGACATAGAACTGCCCTTT+TGG | 0.289844 | 4.3:-20314026 | None:intergenic |
| GAATCAGCATAAGCCCATTT+AGG | 0.291998 | 4.3:-20316496 | None:intergenic |
| ATTTCCAGAGGCGAAGTTAC+TGG | 0.301349 | 4.3:+20312463 | MS.gene70367:CDS |
| GATGGCACAAAAGAAACTTT+AGG | 0.301408 | 4.3:+20318217 | MS.gene70367:CDS |
| TTGGTTGGGGACCTTATTAA+TGG | 0.314186 | 4.3:-20318936 | None:intergenic |
| ATGCGGGCGGATTTCCAAAA+TGG | 0.320508 | 4.3:+20316101 | MS.gene70367:CDS |
| TCCGTCTGTGGACCATTTCC+CGG | 0.320563 | 4.3:-20314871 | None:intergenic |
| GATGGCCAGAAAGGAATTTC+AGG | 0.321998 | 4.3:+20318130 | MS.gene70367:CDS |
| TTCCGCTGAACTACTGGGTT+TGG | 0.324534 | 4.3:+20316165 | MS.gene70367:CDS |
| AGCTGATGGTGTGGAGGATT+TGG | 0.325559 | 4.3:+20319264 | MS.gene70367:CDS |
| CACAGTCTCTGCAATCAAAT+TGG | 0.329502 | 4.3:+20315275 | MS.gene70367:CDS |
| ATTTGCTTGTAGTGAAGTTC+AGG | 0.330996 | 4.3:+20317060 | MS.gene70367:CDS |
| CCTAATCGAAGAGCCATTTC+GGG | 0.334256 | 4.3:-20310280 | None:intergenic |
| CAGCTACACGTTGTCACTTC+TGG | 0.337235 | 4.3:-20316917 | None:intergenic |
| TAACCCTTAAAAGGGCCTTT+TGG | 0.340909 | 4.3:-20319033 | None:intergenic |
| ACCCTCCCCATGTCCAGATT+TGG | 0.343404 | 4.3:-20318488 | None:intergenic |
| TAACTATTCCGCTGAACTAC+TGG | 0.345530 | 4.3:+20316159 | MS.gene70367:CDS |
| CTTCACAAAGTAACTGAAAC+TGG | 0.348831 | 4.3:+20317388 | MS.gene70367:CDS |
| AAGACAGACATTGAACTTGA+TGG | 0.351590 | 4.3:-20315235 | None:intergenic |
| GAAGGCTGGTTGGTTGGTTG+GGG | 0.353127 | 4.3:-20318949 | None:intergenic |
| TGCTGCTCTTGGTTATGATT+GGG | 0.363320 | 4.3:+20317462 | MS.gene70367:CDS |
| TCGATCAGATGCCATTAATA+AGG | 0.366625 | 4.3:+20318925 | MS.gene70367:CDS |
| TTACCTGGTGACCTCTTACA+TGG | 0.367157 | 4.3:-20318811 | None:intergenic |
| GGAAAAGAATTGGATGATAC+AGG | 0.367538 | 4.3:-20315214 | None:intergenic |
| TGAACTTGATGGAAAAGAAT+TGG | 0.370030 | 4.3:-20315224 | None:intergenic |
| GTCCTGTATGTTTGGGGAAG+TGG | 0.371591 | 4.3:+20316940 | MS.gene70367:CDS |
| AAAGAAGTTAAAGTGCTTGT+TGG | 0.371798 | 4.3:-20314105 | None:intergenic |
| TTACGCCCTGCAGAGATATT+AGG | 0.372239 | 4.3:+20311547 | MS.gene70367:CDS |
| GCTCAAAATCAAAAGAAAAT+AGG | 0.374943 | 4.3:-20316228 | None:intergenic |
| CATTTAGCAGACGCCTGTAT+TGG | 0.375253 | 4.3:-20319087 | None:intergenic |
| AGTCTTCCCCTGATATTTCA+TGG | 0.377076 | 4.3:+20316383 | MS.gene70367:CDS |
| TCCGCTGAACTACTGGGTTT+GGG | 0.377091 | 4.3:+20316166 | MS.gene70367:CDS |
| TGTAGCTGGTCCTGTATGTT+TGG | 0.377603 | 4.3:+20316932 | MS.gene70367:CDS |
| CCCGAAATGGCTCTTCGATT+AGG | 0.378336 | 4.3:+20310280 | MS.gene70367:CDS |
| CTGGGTTTGGGTAAAGAAAC+TGG | 0.378745 | 4.3:+20316178 | MS.gene70367:CDS |
| TGTCCACTACTTGGATGTTA+AGG | 0.380969 | 4.3:+20312809 | MS.gene70367:CDS |
| CTTTACTAGAAATGTAGATT+TGG | 0.382097 | 4.3:+20317102 | MS.gene70367:CDS |
| TCTGGCTTTCCTGCAAATTA+CGG | 0.383525 | 4.3:+20314048 | MS.gene70367:CDS |
| CCATGTCCAGCATTCCCTTC+AGG | 0.383542 | 4.3:+20318079 | MS.gene70367:CDS |
| CATTTGGTCCGTGGGAATCC+TGG | 0.387080 | 4.3:+20318853 | MS.gene70367:CDS |
| CGCATGTTACCGTAATTTGC+AGG | 0.387863 | 4.3:-20314057 | None:intergenic |
| GTAGCTGGTCCTGTATGTTT+GGG | 0.391436 | 4.3:+20316933 | MS.gene70367:CDS |
| TTCACTACAAGCAAATCTAT+TGG | 0.391533 | 4.3:-20317053 | None:intergenic |
| CTTTCATGAGTTGCATAATC+CGG | 0.391682 | 4.3:+20314852 | MS.gene70367:CDS |
| CTACATTTAGTTGCTGCTCT+TGG | 0.391917 | 4.3:+20317451 | MS.gene70367:CDS |
| ACACCTGCAGATCTTGCTTC+TGG | 0.393291 | 4.3:+20318106 | MS.gene70367:CDS |
| ATGGCACAAAAGAAACTTTA+GGG | 0.395086 | 4.3:+20318218 | MS.gene70367:CDS |
| TCCGTGCCCCATGAAATATC+AGG | 0.400653 | 4.3:-20316391 | None:intergenic |
| GAATTGCAACGCCTGTGCAC+TGG | 0.401573 | 4.3:+20318269 | MS.gene70367:CDS |
| TATCTTTCATAGGCAGGTTA+AGG | 0.405053 | 4.3:+20317345 | MS.gene70367:CDS |
| TCTCTGACCCTCGTTTGAAT+TGG | 0.406378 | 4.3:-20315125 | None:intergenic |
| ATTCCCACGGACCAAATGAT+TGG | 0.406432 | 4.3:-20318848 | None:intergenic |
| ATTTAGCAGACGCCTGTATT+GGG | 0.411016 | 4.3:-20319086 | None:intergenic |
| AATTGCAACGCCTGTGCACT+GGG | 0.412088 | 4.3:+20318270 | MS.gene70367:CDS |
| CGCTCCAGCAGCTGCACCCA+TGG | 0.413239 | 4.3:-20318047 | None:intergenic |
| GTCTTCCCCTGATATTTCAT+GGG | 0.413274 | 4.3:+20316384 | MS.gene70367:CDS |
| TAATCTGAGTTCGATAAATT+CGG | 0.414296 | 4.3:+20319234 | MS.gene70367:CDS |
| AAGAAGTTAAAGTGCTTGTT+GGG | 0.415064 | 4.3:-20314104 | None:intergenic |
| AGAAGTACAAACCAATCATT+TGG | 0.419166 | 4.3:+20318837 | MS.gene70367:CDS |
| GCTTCTAAGTCATCCAAATC+TGG | 0.420233 | 4.3:+20318475 | MS.gene70367:CDS |
| GGTTGCTCTCCTTGTCTCCA+TGG | 0.421375 | 4.3:+20318030 | MS.gene70367:CDS |
| CCTCATGTGTTTGGTAAAGA+TGG | 0.422095 | 4.3:+20317418 | MS.gene70367:CDS |
| TGAAGGCACATATCTTTCAT+AGG | 0.423985 | 4.3:+20317335 | MS.gene70367:CDS |
| TATAGTATTTGTCCACTACT+TGG | 0.426647 | 4.3:+20312800 | MS.gene70367:CDS |
| GGGAAGGCTGGTTGGTTGGT+TGG | 0.428381 | 4.3:-20318951 | None:intergenic |
| ACTGGAGGAGTTACTTACTT+TGG | 0.436854 | 4.3:+20317168 | MS.gene70367:CDS |
| GCCAGATGCTATATGCCTTA+AGG | 0.440387 | 4.3:+20318300 | MS.gene70367:CDS |
| CAGCGGCTTGTGTAGCATTA+CGG | 0.442930 | 4.3:-20318339 | None:intergenic |
| CAGAAGCTCGGGCCCAATAC+AGG | 0.444672 | 4.3:+20319074 | MS.gene70367:CDS |
| GGTAAAGGGCCTCATGTGTT+TGG | 0.444881 | 4.3:+20317409 | MS.gene70367:CDS |
| CAAGCTCCACCTCATGAGAT+TGG | 0.446603 | 4.3:+20317004 | MS.gene70367:CDS |
| GGCACATATCTTTCATAGGC+AGG | 0.447036 | 4.3:+20317339 | MS.gene70367:CDS |
| TAAAGTGCTTGTTGGGCTCA+TGG | 0.450539 | 4.3:-20314097 | None:intergenic |
| AATGTCATGGATAAATCTTC+AGG | 0.452873 | 4.3:+20315172 | MS.gene70367:CDS |
| TAGAAAATACCTTGGTTTGA+CGG | 0.454767 | 4.3:-20319123 | None:intergenic |
| ACCAACCAGCCTTCCCGAAA+AGG | 0.456564 | 4.3:+20318958 | MS.gene70367:CDS |
| CATCCATTGTGAGTGATTCC+AGG | 0.456642 | 4.3:-20318183 | None:intergenic |
| GTTTGTACTTCTTTCTTACC+TGG | 0.458365 | 4.3:-20318826 | None:intergenic |
| CAGGTTAACAAGACCAATGT+AGG | 0.458647 | 4.3:+20313967 | MS.gene70367:intron |
| TCCTTAAGGCATATAGCATC+TGG | 0.459831 | 4.3:-20318301 | None:intergenic |
| AAAGGATAGTAACCTGAAAA+CGG | 0.462345 | 4.3:-20314934 | None:intergenic |
| TTAACTCCAGAAGTTACAAT+TGG | 0.464038 | 4.3:-20317493 | None:intergenic |
| GAAAACGGGTGCATAAGATA+TGG | 0.464608 | 4.3:-20314919 | None:intergenic |
| AAGGGCAGTTCTATGTCATC+TGG | 0.466818 | 4.3:+20314030 | MS.gene70367:CDS |
| AAGTAACTGAAACTGGTAAA+GGG | 0.469551 | 4.3:+20317395 | MS.gene70367:CDS |
| TGATGACAACTTCTTACCTA+TGG | 0.472509 | 4.3:+20319309 | MS.gene70367:CDS |
| GCAGATCTTGCTTCTGGCGA+TGG | 0.474264 | 4.3:+20318112 | MS.gene70367:CDS |
| GCCTCCAGTAACTTCGCCTC+TGG | 0.477832 | 4.3:-20312467 | None:intergenic |
| TTGGAGAAGGTTACGTAGAA+AGG | 0.479331 | 4.3:-20317034 | None:intergenic |
| CCCTGATATTTCATGGGGCA+CGG | 0.479386 | 4.3:+20316390 | MS.gene70367:CDS |
| GAGTTGCATAATCCGGGAAA+TGG | 0.480366 | 4.3:+20314859 | MS.gene70367:CDS |
| TACCTGGTGACCTCTTACAT+GGG | 0.482192 | 4.3:-20318810 | None:intergenic |
| AAGGATAGTAACCTGAAAAC+GGG | 0.482248 | 4.3:-20314933 | None:intergenic |
| TGCGAAACACTGAGCTTGCT+TGG | 0.482928 | 4.3:-20314828 | None:intergenic |
| CCATTAGCTAAAATCGTAGC+TGG | 0.483148 | 4.3:-20316968 | None:intergenic |
| GGAGCGTTCTTCTCCCGAAA+TGG | 0.484215 | 4.3:+20310267 | MS.gene70367:CDS |
| GTTCTTCCTGAAGGGAATGC+TGG | 0.486036 | 4.3:-20318085 | None:intergenic |
| ATAGCACCAATTGTAACTTC+TGG | 0.488450 | 4.3:+20317487 | MS.gene70367:CDS |
| CCTGAAGGGAATGCTGGACA+TGG | 0.490271 | 4.3:-20318079 | None:intergenic |
| TATTTGTACTGCAGCAGCAT+TGG | 0.491242 | 4.3:-20318515 | None:intergenic |
| ATGGATGGACGGCTCTACAC+TGG | 0.493508 | 4.3:+20317536 | MS.gene70367:CDS |
| AGAATTCCTAATATCTCTGC+AGG | 0.493849 | 4.3:-20311553 | None:intergenic |
| TCAAAGAGTTGTCAAAATCC+AGG | 0.494158 | 4.3:+20318588 | MS.gene70367:CDS |
| CAGCATCAAGAGAATCCTTA+AGG | 0.496736 | 4.3:-20318315 | None:intergenic |
| GAGCACTCAACATAGGGTCC+TGG | 0.496971 | 4.3:+20315146 | MS.gene70367:CDS |
| TCGAGTAATGAAGAGCCAAC+TGG | 0.497413 | 4.3:+20317286 | MS.gene70367:CDS |
| TTGAGGCACTTTAGAAGGGA+TGG | 0.497421 | 4.3:+20312155 | MS.gene70367:CDS |
| CGGTGGAGGCGTAAGGGCAG+TGG | 0.498062 | 4.3:+20318890 | MS.gene70367:CDS |
| GGATGATACAGGGGAAGGTT+CGG | 0.500961 | 4.3:-20315203 | None:intergenic |
| TTTCGGGAAGGCTGGTTGGT+TGG | 0.502036 | 4.3:-20318955 | None:intergenic |
| AATGCGACATCATTTGCAAC+TGG | 0.503844 | 4.3:+20317150 | MS.gene70367:CDS |
| AACTGGCGAAAGAAGAATGA+TGG | 0.504245 | 4.3:+20312182 | MS.gene70367:CDS |
| TATATTACGGTGGAGGCGTA+AGG | 0.504437 | 4.3:+20318883 | MS.gene70367:CDS |
| TTATGCTGATTCAGAAATTG+AGG | 0.506793 | 4.3:+20316507 | MS.gene70367:CDS |
| GAAATATCCATCTCTCCAGT+TGG | 0.507509 | 4.3:-20317301 | None:intergenic |
| AGAAGTTACAATTGGTGCTA+TGG | 0.508586 | 4.3:-20317485 | None:intergenic |
| TGTGATAGATGATACCTCAT+TGG | 0.509091 | 4.3:+20316426 | MS.gene70367:CDS |
| TTTATCCATGACATTGTCCC+AGG | 0.509557 | 4.3:-20315164 | None:intergenic |
| GTCTCTGCAATCAAATTGGC+AGG | 0.510338 | 4.3:+20315279 | MS.gene70367:CDS |
| TCCTGGAGAAGGTTATATTA+CGG | 0.511577 | 4.3:+20318870 | MS.gene70367:CDS |
| GTTGCTCTCCTTGTCTCCAT+GGG | 0.517325 | 4.3:+20318031 | MS.gene70367:CDS |
| CTACACTGGGCTGCATCATG+TGG | 0.518678 | 4.3:+20317550 | MS.gene70367:CDS |
| ATGTTAAAGGCCCATGTAAG+AGG | 0.521276 | 4.3:+20318800 | MS.gene70367:intron |
| CATGGACTTAACCCTTAAAA+GGG | 0.522222 | 4.3:-20319041 | None:intergenic |
| TTCCACTTCCCCAAACATAC+AGG | 0.522582 | 4.3:-20316942 | None:intergenic |
| TAGAAACCATACTCGGTGGC+CGG | 0.524497 | 4.3:-20311618 | None:intergenic |
| CAGAAGTGACAACGTGTAGC+TGG | 0.528053 | 4.3:+20316918 | MS.gene70367:CDS |
| CTCCACCTCATGAGATTGGA+CGG | 0.528271 | 4.3:+20317008 | MS.gene70367:CDS |
| AAAGTAACTGAAACTGGTAA+AGG | 0.528379 | 4.3:+20317394 | MS.gene70367:CDS |
| TTAGAAGGGATGGATACAAC+TGG | 0.529528 | 4.3:+20312165 | MS.gene70367:CDS |
| AAATCAAAAGAAAATAGGTC+AGG | 0.530279 | 4.3:-20316223 | None:intergenic |
| TTTCATGAGTTGCATAATCC+GGG | 0.531246 | 4.3:+20314853 | MS.gene70367:CDS |
| AGGCATAAGTACAGATACTA+AGG | 0.532801 | 4.3:+20313987 | MS.gene70367:CDS |
| TTGCAACGCCTGTGCACTGG+GGG | 0.533191 | 4.3:+20318272 | MS.gene70367:CDS |
| CAGGAATTTGAGTACAAAGT+GGG | 0.533273 | 4.3:+20317079 | MS.gene70367:CDS |
| TGAAGAGCCAACTGGAGAGA+TGG | 0.533599 | 4.3:+20317294 | MS.gene70367:CDS |
| ATCAACTTCCGTGATGTGAA+TGG | 0.534863 | 4.3:+20317517 | MS.gene70367:CDS |
| CTCATGTGTTTGGTAAAGAT+GGG | 0.535877 | 4.3:+20317419 | MS.gene70367:CDS |
| TCGCCAGAAGCAAGATCTGC+AGG | 0.537099 | 4.3:-20318109 | None:intergenic |
| TCTGTCTTCAACGAAGGAAG+TGG | 0.537368 | 4.3:+20315250 | MS.gene70367:CDS |
| ACTGGGCTGCATCATGTGGC+AGG | 0.538074 | 4.3:+20317554 | MS.gene70367:CDS |
| ACCATACTCGGTGGCCGGGT+AGG | 0.538324 | 4.3:-20311613 | None:intergenic |
| GCTTCTGGCGATGGCCAGAA+AGG | 0.539832 | 4.3:+20318121 | MS.gene70367:CDS |
| TTACCTCCCACTTGTTCAGT+GGG | 0.542639 | 4.3:+20315096 | MS.gene70367:CDS |
| GGTCAGAGAGCACTCAACAT+AGG | 0.543054 | 4.3:+20315139 | MS.gene70367:CDS |
| AGAAACCATACTCGGTGGCC+GGG | 0.543565 | 4.3:-20311617 | None:intergenic |
| TGGTTCAGTATCCAGAAGCT+CGG | 0.545136 | 4.3:+20319062 | MS.gene70367:CDS |
| GTCAGAGAGCACTCAACATA+GGG | 0.545853 | 4.3:+20315140 | MS.gene70367:CDS |
| GGTTACATCAGATTCCCAAA+AGG | 0.546119 | 4.3:+20314011 | MS.gene70367:CDS |
| GGTTCAGTATCCAGAAGCTC+GGG | 0.547885 | 4.3:+20319063 | MS.gene70367:CDS |
| TATTTCAAAACACAGGTTGA+AGG | 0.548307 | 4.3:+20317318 | MS.gene70367:CDS |
| ACCGTAATATAACCTTCTCC+AGG | 0.551191 | 4.3:-20318871 | None:intergenic |
| ATCCAAATCTGGACATGGGG+AGG | 0.552979 | 4.3:+20318486 | MS.gene70367:CDS |
| TCAGTATGAAGAGGATGATG+AGG | 0.553757 | 4.3:+20318414 | MS.gene70367:CDS |
| AGTCATGTATGCTAAACAGC+TGG | 0.554108 | 4.3:-20316468 | None:intergenic |
| GGAAGCTCATGAGAAGTTGA+AGG | 0.554386 | 4.3:+20312217 | MS.gene70367:CDS |
| GCTGGTCTTGAGATAGAGAC+AGG | 0.556085 | 4.3:-20316450 | None:intergenic |
| CGGATTATGCAACTCATGAA+AGG | 0.556996 | 4.3:-20314851 | None:intergenic |
| AGTTTGAAGCACAGCATCGA+TGG | 0.557059 | 4.3:+20311524 | MS.gene70367:CDS |
| TCTGCAGGTGTTCTTCCTGA+AGG | 0.557364 | 4.3:-20318094 | None:intergenic |
| GAGTGAACAAAAGTTCCAAA+AGG | 0.557664 | 4.3:+20319018 | MS.gene70367:CDS |
| GGCCCATGTAAGAGGTCACC+AGG | 0.559386 | 4.3:+20318808 | MS.gene70367:intron |
| CTTTACTCAGTCGTTAAGCA+TGG | 0.560266 | 4.3:+20316126 | MS.gene70367:CDS |
| ATTACCTCCCACTTGTTCAG+TGG | 0.560370 | 4.3:+20315095 | MS.gene70367:CDS |
| GATTTACCCCACTGAACAAG+TGG | 0.561100 | 4.3:-20315103 | None:intergenic |
| AACTATTCCGCTGAACTACT+GGG | 0.561115 | 4.3:+20316160 | MS.gene70367:CDS |
| GTTACATCAGATTCCCAAAA+GGG | 0.561877 | 4.3:+20314012 | MS.gene70367:CDS |
| GAGTTCGATAAATTCGGAAG+AGG | 0.562641 | 4.3:+20319240 | MS.gene70367:CDS |
| TCTCAAAAGAACCGCCTACC+CGG | 0.563454 | 4.3:+20311599 | MS.gene70367:CDS |
| AGCACTCAACATAGGGTCCT+GGG | 0.567035 | 4.3:+20315147 | MS.gene70367:CDS |
| GATTAAAGAATTTGCATCAG+TGG | 0.567503 | 4.3:+20316345 | MS.gene70367:CDS |
| CCGTGCCCCATGAAATATCA+GGG | 0.568861 | 4.3:-20316390 | None:intergenic |
| ATGGATGATGTGAATAAAGA+TGG | 0.571903 | 4.3:+20318199 | MS.gene70367:CDS |
| ATACTCGGTGGCCGGGTAGG+CGG | 0.571941 | 4.3:-20311610 | None:intergenic |
| TCAATGTCTGTCTTCAACGA+AGG | 0.572676 | 4.3:+20315244 | MS.gene70367:CDS |
| GTCATTATTAACTAGCCACC+TGG | 0.573370 | 4.3:+20318165 | MS.gene70367:CDS |
| CAGCTGCACCCATGGAGACA+AGG | 0.573677 | 4.3:-20318039 | None:intergenic |
| CGTAAGGGCAGTGGTTTACG+TGG | 0.575467 | 4.3:+20318899 | MS.gene70367:CDS |
| TTAAGGAGAAGCTGTACTCA+TGG | 0.575527 | 4.3:+20317362 | MS.gene70367:CDS |
| AACCCATTTGAAGAAAATGC+GGG | 0.575833 | 4.3:+20316085 | MS.gene70367:CDS |
| CTGCAGGTGTTCTTCCTGAA+GGG | 0.576107 | 4.3:-20318093 | None:intergenic |
| TCCAAATCTGGACATGGGGA+GGG | 0.579121 | 4.3:+20318487 | MS.gene70367:CDS |
| TGGATGGACGGCTCTACACT+GGG | 0.581082 | 4.3:+20317537 | MS.gene70367:CDS |
| TACTTGATGTATTCTATCAG+CGG | 0.582154 | 4.3:-20318356 | None:intergenic |
| ACCCGTCCAATCTCATGAGG+TGG | 0.583328 | 4.3:-20317010 | None:intergenic |
| CAGGCGTCTGCTAAATGTAG+TGG | 0.586819 | 4.3:+20319093 | MS.gene70367:CDS |
| AAGTCATCCAAATCTGGACA+TGG | 0.586904 | 4.3:+20318481 | MS.gene70367:CDS |
| GTCCGTGGGAATCCTGGAGA+AGG | 0.588343 | 4.3:+20318859 | MS.gene70367:CDS |
| AGGGTCCTGGGACAATGTCA+TGG | 0.588470 | 4.3:+20315159 | MS.gene70367:CDS |
| ACAAGCAAATCTATTGGAGA+AGG | 0.590474 | 4.3:-20317047 | None:intergenic |
| AAGGATCAATCGAATGCCAT+AGG | 0.590654 | 4.3:-20319325 | None:intergenic |
| AAACCAATCATTTGGTCCGT+GGG | 0.591404 | 4.3:+20318845 | MS.gene70367:CDS |
| GAAAAGAATTGGATGATACA+GGG | 0.592068 | 4.3:-20315213 | None:intergenic |
| ATCTGTACTTATGCCTACAT+TGG | 0.592564 | 4.3:-20313980 | None:intergenic |
| TCCGGGAAATGGTCCACAGA+CGG | 0.593297 | 4.3:+20314870 | MS.gene70367:CDS |
| GCAGCTAGCTCAGTATGAAG+AGG | 0.595976 | 4.3:+20318405 | MS.gene70367:CDS |
| ATCTTTCAAACATAGGTGGA+AGG | 0.598899 | 4.3:+20312393 | MS.gene70367:intron |
| TCTTCCCCTGATATTTCATG+GGG | 0.604539 | 4.3:+20316385 | MS.gene70367:CDS |
| TTACCTTAACATCCAAGTAG+TGG | 0.606730 | 4.3:-20312812 | None:intergenic |
| CAAACCAATCATTTGGTCCG+TGG | 0.607032 | 4.3:+20318844 | MS.gene70367:CDS |
| TCCACCTCATGAGATTGGAC+GGG | 0.608713 | 4.3:+20317009 | MS.gene70367:CDS |
| AGAAGGTTATATTACGGTGG+AGG | 0.609021 | 4.3:+20318876 | MS.gene70367:CDS |
| ATACACTGCTACTACGCCCA+TGG | 0.609101 | 4.3:+20312426 | MS.gene70367:CDS |
| AACCAGCCTTCCCGAAAAGG+AGG | 0.610047 | 4.3:+20318961 | MS.gene70367:CDS |
| ATATTACGGTGGAGGCGTAA+GGG | 0.610245 | 4.3:+20318884 | MS.gene70367:CDS |
| AATTCGGAAGAGGCAGCTGA+TGG | 0.613503 | 4.3:+20319250 | MS.gene70367:CDS |
| AGTCATCCAAATCTGGACAT+GGG | 0.619839 | 4.3:+20318482 | MS.gene70367:CDS |
| GTCATCCAAATCTGGACATG+GGG | 0.620280 | 4.3:+20318483 | MS.gene70367:CDS |
| TTATATGCTCAGAGAGAGAA+CGG | 0.620970 | 4.3:+20318009 | MS.gene70367:intron |
| AGATGGATATTTCAAAACAC+AGG | 0.621087 | 4.3:+20317311 | MS.gene70367:CDS |
| ACTTCCGTGATGTGAATGGA+TGG | 0.622379 | 4.3:+20317521 | MS.gene70367:CDS |
| CCGTCCATCCATTCACATCA+CGG | 0.622728 | 4.3:-20317525 | None:intergenic |
| TCCAGAGGCGAAGTTACTGG+AGG | 0.623827 | 4.3:+20312466 | MS.gene70367:CDS |
| GGAAGAGGCAGCTGATGGTG+TGG | 0.625414 | 4.3:+20319255 | MS.gene70367:CDS |
| TCATGGGGCACGGATGAATG+TGG | 0.628341 | 4.3:+20316400 | MS.gene70367:CDS |
| ACCCAAACCCAGTAGTTCAG+CGG | 0.628373 | 4.3:-20316167 | None:intergenic |
| TCAGGAATTTGAGTACAAAG+TGG | 0.630025 | 4.3:+20317078 | MS.gene70367:CDS |
| GAACCCATTTGAAGAAAATG+CGG | 0.632228 | 4.3:+20316084 | MS.gene70367:intron |
| CCGTGATGTGAATGGATGGA+CGG | 0.639667 | 4.3:+20317525 | MS.gene70367:CDS |
| AACCTTCTCCAGGATTCCCA+CGG | 0.641059 | 4.3:-20318861 | None:intergenic |
| GAATTGGATGATACAGGGGA+AGG | 0.641814 | 4.3:-20315208 | None:intergenic |
| GCATCAATCTTATCCGTCTG+TGG | 0.641952 | 4.3:-20314883 | None:intergenic |
| GCGACATCATTTGCAACTGG+AGG | 0.643033 | 4.3:+20317153 | MS.gene70367:CDS |
| AGAGGCAGCTGATGGTGTGG+AGG | 0.643223 | 4.3:+20319258 | MS.gene70367:CDS |
| CCACCTGGAATCACTCACAA+TGG | 0.644430 | 4.3:+20318180 | MS.gene70367:CDS |
| AAAAGAATTGGATGATACAG+GGG | 0.645982 | 4.3:-20315212 | None:intergenic |
| CCATTTGAAGAAAATGCGGG+CGG | 0.658580 | 4.3:+20316088 | MS.gene70367:CDS |
| GTGGCTAGTTAATAATGACT+CGG | 0.659900 | 4.3:-20318161 | None:intergenic |
| GGAACCCGTCCAATCTCATG+AGG | 0.671886 | 4.3:-20317013 | None:intergenic |
| AGAAGCTGTCAACCTTCGTG+AGG | 0.672119 | 4.3:-20316315 | None:intergenic |
| CATAAGTACAGATACTAAGG+AGG | 0.672927 | 4.3:+20313990 | MS.gene70367:CDS |
| AAGATAATGAAAATTTCCAG+AGG | 0.673367 | 4.3:+20312451 | MS.gene70367:CDS |
| GGGTAAATCCAATTCAAACG+AGG | 0.675725 | 4.3:+20315117 | MS.gene70367:CDS |
| CCATCTTTACCAAACACATG+AGG | 0.676075 | 4.3:-20317418 | None:intergenic |
| GGTAAATCCAATTCAAACGA+GGG | 0.679092 | 4.3:+20315118 | MS.gene70367:CDS |
| AGATAGAGACAGGTCCAATG+AGG | 0.681492 | 4.3:-20316440 | None:intergenic |
| ATTGCAACGCCTGTGCACTG+GGG | 0.681592 | 4.3:+20318271 | MS.gene70367:CDS |
| TGGAGAAGGTTATATTACGG+TGG | 0.685543 | 4.3:+20318873 | MS.gene70367:CDS |
| GAATTCCTAATATCTCTGCA+GGG | 0.686153 | 4.3:-20311552 | None:intergenic |
| GGATGATTACCGTCAAACCA+AGG | 0.687154 | 4.3:+20319114 | MS.gene70367:CDS |
| AGCTTCTGGATACTGAACCA+TGG | 0.689349 | 4.3:-20319059 | None:intergenic |
| TGCTAAAGAGAGCCTCACGA+AGG | 0.690094 | 4.3:+20316303 | MS.gene70367:CDS |
| TAGCTGGTCCTGTATGTTTG+GGG | 0.692929 | 4.3:+20316934 | MS.gene70367:CDS |
| ATTTACCCCACTGAACAAGT+GGG | 0.693252 | 4.3:-20315102 | None:intergenic |
| TACCTCCCACTTGTTCAGTG+GGG | 0.703440 | 4.3:+20315097 | MS.gene70367:CDS |
| TACCCCACTGAACAAGTGGG+AGG | 0.704031 | 4.3:-20315099 | None:intergenic |
| CGTGCCCCATGAAATATCAG+GGG | 0.706527 | 4.3:-20316389 | None:intergenic |
| GTGTTTGGTAAAGATGGGCA+AGG | 0.707666 | 4.3:+20317424 | MS.gene70367:CDS |
| TGTGCATCACAACTTGCACA+CGG | 0.708380 | 4.3:+20316264 | MS.gene70367:CDS |
| TGCAACGCCTGTGCACTGGG+GGG | 0.729574 | 4.3:+20318273 | MS.gene70367:CDS |
| CCATTGTGAGTGATTCCAGG+TGG | 0.730255 | 4.3:-20318180 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 20310262 | 20319342 | 20310262 | ID=MS.gene70367 |
| chr4.3 | mRNA | 20310262 | 20319342 | 20310262 | ID=MS.gene70367.t1;Parent=MS.gene70367 |
| chr4.3 | exon | 20310262 | 20310301 | 20310262 | ID=MS.gene70367.t1.exon1;Parent=MS.gene70367.t1 |
| chr4.3 | CDS | 20310262 | 20310301 | 20310262 | ID=cds.MS.gene70367.t1;Parent=MS.gene70367.t1 |
| chr4.3 | exon | 20311509 | 20311628 | 20311509 | ID=MS.gene70367.t1.exon2;Parent=MS.gene70367.t1 |
| chr4.3 | CDS | 20311509 | 20311628 | 20311509 | ID=cds.MS.gene70367.t1;Parent=MS.gene70367.t1 |
| chr4.3 | exon | 20312123 | 20312238 | 20312123 | ID=MS.gene70367.t1.exon3;Parent=MS.gene70367.t1 |
| chr4.3 | CDS | 20312123 | 20312238 | 20312123 | ID=cds.MS.gene70367.t1;Parent=MS.gene70367.t1 |
| chr4.3 | exon | 20312408 | 20312496 | 20312408 | ID=MS.gene70367.t1.exon4;Parent=MS.gene70367.t1 |
| chr4.3 | CDS | 20312408 | 20312496 | 20312408 | ID=cds.MS.gene70367.t1;Parent=MS.gene70367.t1 |
| chr4.3 | exon | 20312788 | 20312830 | 20312788 | ID=MS.gene70367.t1.exon5;Parent=MS.gene70367.t1 |
| chr4.3 | CDS | 20312788 | 20312830 | 20312788 | ID=cds.MS.gene70367.t1;Parent=MS.gene70367.t1 |
| chr4.3 | exon | 20313970 | 20314147 | 20313970 | ID=MS.gene70367.t1.exon6;Parent=MS.gene70367.t1 |
| chr4.3 | CDS | 20313970 | 20314147 | 20313970 | ID=cds.MS.gene70367.t1;Parent=MS.gene70367.t1 |
| chr4.3 | exon | 20314818 | 20314943 | 20314818 | ID=MS.gene70367.t1.exon7;Parent=MS.gene70367.t1 |
| chr4.3 | CDS | 20314818 | 20314943 | 20314818 | ID=cds.MS.gene70367.t1;Parent=MS.gene70367.t1 |
| chr4.3 | exon | 20315089 | 20315300 | 20315089 | ID=MS.gene70367.t1.exon8;Parent=MS.gene70367.t1 |
| chr4.3 | CDS | 20315089 | 20315300 | 20315089 | ID=cds.MS.gene70367.t1;Parent=MS.gene70367.t1 |
| chr4.3 | exon | 20316085 | 20316528 | 20316085 | ID=MS.gene70367.t1.exon9;Parent=MS.gene70367.t1 |
| chr4.3 | CDS | 20316085 | 20316528 | 20316085 | ID=cds.MS.gene70367.t1;Parent=MS.gene70367.t1 |
| chr4.3 | exon | 20316884 | 20317575 | 20316884 | ID=MS.gene70367.t1.exon10;Parent=MS.gene70367.t1 |
| chr4.3 | CDS | 20316884 | 20317575 | 20316884 | ID=cds.MS.gene70367.t1;Parent=MS.gene70367.t1 |
| chr4.3 | exon | 20318021 | 20318609 | 20318021 | ID=MS.gene70367.t1.exon11;Parent=MS.gene70367.t1 |
| chr4.3 | CDS | 20318021 | 20318609 | 20318021 | ID=cds.MS.gene70367.t1;Parent=MS.gene70367.t1 |
| chr4.3 | exon | 20318809 | 20319135 | 20318809 | ID=MS.gene70367.t1.exon12;Parent=MS.gene70367.t1 |
| chr4.3 | CDS | 20318809 | 20319135 | 20318809 | ID=cds.MS.gene70367.t1;Parent=MS.gene70367.t1 |
| chr4.3 | exon | 20319229 | 20319342 | 20319229 | ID=MS.gene70367.t1.exon13;Parent=MS.gene70367.t1 |
| chr4.3 | CDS | 20319229 | 20319342 | 20319229 | ID=cds.MS.gene70367.t1;Parent=MS.gene70367.t1 |
| Gene Sequence |
| Protein sequence |
