AlfalfaGEDB | MS.gene71614

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71614.t1	XP_013469983.1	93.4	76	5	0	1	76	38	113	3.60E-31	143.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71614.t1	A0A072VQS7	93.4	76	5	0	1	76	38	113	2.6e-31	143.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049573	MS.gene71614	0.818622	1.78E-52	-1.69E-46
MS.gene05142	MS.gene71614	0.806011	1.01E-49	-1.69E-46
MS.gene051946	MS.gene71614	0.851053	1.14E-60	-1.69E-46
MS.gene055401	MS.gene71614	0.830683	2.56E-55	-1.69E-46
MS.gene056310	MS.gene71614	0.813716	2.22E-51	-1.69E-46
MS.gene056311	MS.gene71614	0.816486	5.40E-52	-1.69E-46
MS.gene056313	MS.gene71614	0.809359	1.97E-50	-1.69E-46
MS.gene057014	MS.gene71614	0.804427	2.17E-49	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene71614.t1	MTR_1g106215	93.421	76	5	0	1	76	38	113	3.71e-48	148

Find 23 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCGTAGCTACCACATGTTAT+TGG	0.297025	1.4:-82203887	None:intergenic
ATTTGGTAATGATGGAAGTT+TGG	0.326402	1.4:-82203782	MS.gene71614:CDS
TTGGAATCTGACTCATTAAC+AGG	0.333936	1.4:-82203868	MS.gene71614:CDS
GAGCACAGATTTGGTAATGA+TGG	0.406744	1.4:-82203790	MS.gene71614:CDS
AGCATAAGAAGCAGCAGCAC+TGG	0.414803	1.4:+82203714	None:intergenic
AGCTGTCACAATGATGCCTC+TGG	0.415269	1.4:+82203753	None:intergenic
GAGTTCTTAGAGCACAGATT+TGG	0.435020	1.4:-82203799	MS.gene71614:CDS
TGAAATTCTTTGAGGGTGGT+TGG	0.465990	1.4:+82203689	None:intergenic
TGCTCTAAGAACTCAGTTGT+GGG	0.467329	1.4:+82203808	None:intergenic
GAAATTCTTTGAGGGTGGTT+GGG	0.475402	1.4:+82203690	None:intergenic
ATGTTGTTGAAATTCTTTGA+GGG	0.507140	1.4:+82203682	None:intergenic
GCTGTCACAATGATGCCTCT+GGG	0.532008	1.4:+82203754	None:intergenic
GCAGCACTGGCAGTGGTCGG+AGG	0.539102	1.4:+82203727	None:intergenic
GTGCTCTAAGAACTCAGTTG+TGG	0.553458	1.4:+82203807	None:intergenic
GCAGCAGCACTGGCAGTGGT+CGG	0.562924	1.4:+82203724	None:intergenic
AATGTTGTTGAAATTCTTTG+AGG	0.567996	1.4:+82203681	None:intergenic
AGAAGCAGCAGCACTGGCAG+TGG	0.577609	1.4:+82203720	None:intergenic
TTGTTGAAATTCTTTGAGGG+TGG	0.597370	1.4:+82203685	None:intergenic
AGACCTGGTTCTTCTATGAG+AGG	0.610651	1.4:+82203843	None:intergenic
GGAAGTTTGGTTAGCCCCAG+AGG	0.621319	1.4:-82203769	MS.gene71614:CDS
CTGTCACAATGATGCCTCTG+GGG	0.650846	1.4:+82203755	None:intergenic
GATCCTCTCATAGAAGAACC+AGG	0.662887	1.4:-82203846	MS.gene71614:CDS
GGGTTGCTGAAAATCAGACC+TGG	0.666499	1.4:+82203828	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	AATGTTGTTGAAATTCTTTG+AGG	+	chr1.4:82203862-82203881	None:intergenic	25.0%
!	ATGTTGTTGAAATTCTTTGA+GGG	+	chr1.4:82203861-82203880	None:intergenic	25.0%
!!	ATTTGGTAATGATGGAAGTT+TGG	-	chr1.4:82203758-82203777	MS.gene71614:CDS	30.0%
	TTGGAATCTGACTCATTAAC+AGG	-	chr1.4:82203672-82203691	MS.gene71614:CDS	35.0%
!	TTGTTGAAATTCTTTGAGGG+TGG	+	chr1.4:82203858-82203877	None:intergenic	35.0%
	GAGTTCTTAGAGCACAGATT+TGG	-	chr1.4:82203741-82203760	MS.gene71614:CDS	40.0%
!	GAAATTCTTTGAGGGTGGTT+GGG	+	chr1.4:82203853-82203872	None:intergenic	40.0%
!	TGAAATTCTTTGAGGGTGGT+TGG	+	chr1.4:82203854-82203873	None:intergenic	40.0%
!!	GAGCACAGATTTGGTAATGA+TGG	-	chr1.4:82203750-82203769	MS.gene71614:CDS	40.0%
!!	TGCTCTAAGAACTCAGTTGT+GGG	+	chr1.4:82203735-82203754	None:intergenic	40.0%
	GATCCTCTCATAGAAGAACC+AGG	-	chr1.4:82203694-82203713	MS.gene71614:CDS	45.0%
!	AGACCTGGTTCTTCTATGAG+AGG	+	chr1.4:82203700-82203719	None:intergenic	45.0%
!!	GTGCTCTAAGAACTCAGTTG+TGG	+	chr1.4:82203736-82203755	None:intergenic	45.0%
	AGCATAAGAAGCAGCAGCAC+TGG	+	chr1.4:82203829-82203848	None:intergenic	50.0%
	AGCTGTCACAATGATGCCTC+TGG	+	chr1.4:82203790-82203809	None:intergenic	50.0%
	CTGTCACAATGATGCCTCTG+GGG	+	chr1.4:82203788-82203807	None:intergenic	50.0%
	GCTGTCACAATGATGCCTCT+GGG	+	chr1.4:82203789-82203808	None:intergenic	50.0%
	GGGTTGCTGAAAATCAGACC+TGG	+	chr1.4:82203715-82203734	None:intergenic	50.0%
!	GGAAGTTTGGTTAGCCCCAG+AGG	-	chr1.4:82203771-82203790	MS.gene71614:CDS	55.0%
!!	AGAAGCAGCAGCACTGGCAG+TGG	+	chr1.4:82203823-82203842	None:intergenic	60.0%
!!	GCAGCAGCACTGGCAGTGGT+CGG	+	chr1.4:82203819-82203838	None:intergenic	65.0%
!!	GCAGCACTGGCAGTGGTCGG+AGG	+	chr1.4:82203816-82203835	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	82203666	82203896	82203666	ID=MS.gene71614
chr1.4	mRNA	82203666	82203896	82203666	ID=MS.gene71614.t1;Parent=MS.gene71614
chr1.4	exon	82203666	82203896	82203666	ID=MS.gene71614.t1.exon1;Parent=MS.gene71614.t1
chr1.4	CDS	82203666	82203896	82203666	ID=cds.MS.gene71614.t1;Parent=MS.gene71614.t1

Gene Sequence	>MS.gene71614 ATGTTATTGGAATCTGACTCATTAACAGGATCCTCTCATAGAAGAACCAGGTCTGATTTTCAGCAACCCACAACTGAGTTCTTAGAGCACAGATTTGGTAATGATGGAAGTTTGGTTAGCCCCAGAGGCATCATTGTGACAGCTCTGCCTCCGACCACTGCCAGTGCTGCTGCTTCTTATGCTACCCAACCACCCTCAAAGAATTTCAACAACATTCGCAAACACAAATGA
Protein sequence	>MS.gene71614.t1 MLLESDSLTGSSHRRTRSDFQQPTTEFLEHRFGNDGSLVSPRGIIVTALPPTTASAAASYATQPPSKNFNNIRKHK

M. sativa cultivar XinJiangDaYe / MS.gene71614