Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72510.t1 | RHN59829.1 | 99.5 | 397 | 2 | 0 | 1 | 397 | 1 | 397 | 1.20E-215 | 758.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72510.t1 | Q8L5U0 | 84.1 | 397 | 63 | 0 | 1 | 397 | 1 | 397 | 7.4e-185 | 647.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72510.t1 | A0A396I2I5 | 99.5 | 397 | 2 | 0 | 1 | 397 | 1 | 397 | 8.9e-216 | 758.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene42526 | MS.gene72510 | PPI |
| MS.gene018309 | MS.gene72510 | PPI |
| MS.gene72510 | MS.gene014112 | PPI |
| MS.gene72510 | MS.gene39043 | PPI |
| MS.gene72510 | MS.gene86150 | PPI |
| MS.gene72510 | MS.gene018310 | PPI |
| MS.gene72510 | MS.gene017884 | PPI |
| MS.gene72510 | MS.gene37293 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72510.t1 | MTR_0173s0030 | 99.244 | 397 | 3 | 0 | 1 | 397 | 1 | 397 | 0.0 | 802 |
| MS.gene72510.t1 | MTR_2g034320 | 46.591 | 88 | 28 | 4 | 97 | 183 | 20 | 89 | 5.23e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene72510.t1 | AT5G42970 | 84.131 | 397 | 63 | 0 | 1 | 397 | 1 | 397 | 0.0 | 686 |
Find 76 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTTATATATCAGGTGTATT+TGG | 0.162807 | 4.1:-64012973 | MS.gene72510:intron |
| TCTGCAAAAGCATCAATTTC+AGG | 0.220902 | 4.1:+64012933 | None:intergenic |
| TTTGGTTATTAGAGAGAAAT+TGG | 0.222768 | 4.1:-64016130 | MS.gene72510:CDS |
| ACTGTTCTATCTTCTGTCTT+TGG | 0.232195 | 4.1:+64018591 | None:intergenic |
| CAAATTGAGAAAAGGCAAAT+AGG | 0.242371 | 4.1:-64015357 | MS.gene72510:CDS |
| CATTAATAAAGCATCATTCT+TGG | 0.283000 | 4.1:-64015589 | MS.gene72510:CDS |
| GGTCAATACCACTGAGCATC+TGG | 0.308345 | 4.1:+64016067 | None:intergenic |
| TCAAGCTCTAAATGATGTTT+TGG | 0.311421 | 4.1:-64011451 | MS.gene72510:CDS |
| GAGAGAAACCTTCTGCAGTA+TGG | 0.365955 | 4.1:+64013061 | None:intergenic |
| AGATCTGAAGAGGAAGTTCT+TGG | 0.369986 | 4.1:-64015409 | MS.gene72510:CDS |
| AAATTGAGAAAAGGCAAATA+GGG | 0.370512 | 4.1:-64015356 | MS.gene72510:CDS |
| TCCAGACAATTTCACTGTGC+TGG | 0.378815 | 4.1:-64012804 | MS.gene72510:CDS |
| GCAAACTTTATACAAATATC+AGG | 0.384105 | 4.1:-64012745 | MS.gene72510:intron |
| TCTTGTAGCTTTGATGAGTT+AGG | 0.384222 | 4.1:-64012626 | MS.gene72510:intron |
| TGCCGTTACGTGTACAATAT+TGG | 0.385486 | 4.1:-64013246 | MS.gene72510:CDS |
| GGCAATAATCCAAGTCAAAC+AGG | 0.393367 | 4.1:+64011399 | None:intergenic |
| GCAATCTCTTTCTGTGTCTC+AGG | 0.418879 | 4.1:+64017912 | None:intergenic |
| TGCTGAAGAGTTGGGAAGGT+TGG | 0.443905 | 4.1:-64017937 | MS.gene72510:CDS |
| TCCAGCACAGTGAAATTGTC+TGG | 0.443989 | 4.1:+64012803 | None:intergenic |
| AATCCAAGTCAAACAGGAAC+AGG | 0.449263 | 4.1:+64011405 | None:intergenic |
| TGTACAATATTGGCAGGTGC+AGG | 0.482785 | 4.1:-64013236 | MS.gene72510:CDS |
| AGCATCAAGAATGATTTATG+AGG | 0.485288 | 4.1:-64012170 | MS.gene72510:CDS |
| CAGTGAAATTGTCTGGAAGA+AGG | 0.485991 | 4.1:+64012810 | None:intergenic |
| AGTATTGAATTTGAAGTACA+AGG | 0.490159 | 4.1:-64015547 | MS.gene72510:intron |
| TGTATTTGGAAAGAATTCTT+CGG | 0.492392 | 4.1:-64012959 | MS.gene72510:CDS |
| AGTGGTATTGACCTAGATTC+AGG | 0.492697 | 4.1:-64016057 | MS.gene72510:CDS |
| GATGAGTTAGGCACACTGTT+GGG | 0.493284 | 4.1:-64012614 | MS.gene72510:CDS |
| GATTTATGAGGATAGGATGA+AGG | 0.501314 | 4.1:-64012158 | MS.gene72510:CDS |
| GGGTAATTGATGATAAGTTC+AGG | 0.503152 | 4.1:-64015919 | MS.gene72510:CDS |
| AAATTATGTACCTTGGGAGG+TGG | 0.506288 | 4.1:+64012587 | None:intergenic |
| GAAATTAATGAGGAAGCGCT+TGG | 0.507602 | 4.1:-64013284 | MS.gene72510:CDS |
| ACACTGAAGAGTTGCAAAGA+TGG | 0.510433 | 4.1:-64011984 | MS.gene72510:CDS |
| ACATATCTCAAATTGAGAAA+AGG | 0.510694 | 4.1:-64015365 | MS.gene72510:CDS |
| GATGAAGGGATCTATTGATC+AGG | 0.510987 | 4.1:-64012143 | MS.gene72510:intron |
| AGAAGAATTGAAACCACATC+AGG | 0.513961 | 4.1:-64012913 | MS.gene72510:intron |
| TGTTCTTGCCACACTGTACA+AGG | 0.521177 | 4.1:-64013198 | MS.gene72510:intron |
| GAGACCTCATTCCTGAATCT+AGG | 0.525371 | 4.1:+64016046 | None:intergenic |
| AGTGAAATTGTCTGGAAGAA+GGG | 0.526038 | 4.1:+64012811 | None:intergenic |
| AGAGAAACCTTCTGCAGTAT+GGG | 0.529187 | 4.1:+64013062 | None:intergenic |
| TTGGATAGCATGGGCAAGAA+GGG | 0.533962 | 4.1:-64011432 | MS.gene72510:CDS |
| CAAACTTTAATAACCTGATG+TGG | 0.534249 | 4.1:+64012900 | None:intergenic |
| AAATTAATGAGGAAGCGCTT+GGG | 0.534615 | 4.1:-64013283 | MS.gene72510:CDS |
| CACTGAAGAGTTGCAAAGAT+GGG | 0.536188 | 4.1:-64011983 | MS.gene72510:CDS |
| AATTGCTTGTTTATACCTTG+AGG | 0.536370 | 4.1:-64015879 | MS.gene72510:intron |
| TCAAATTCAATACTTCATGC+TGG | 0.537152 | 4.1:+64015556 | None:intergenic |
| GTTGCAAAGATGGGACCAGC+AGG | 0.537433 | 4.1:-64011974 | MS.gene72510:intron |
| TTTGGATAGCATGGGCAAGA+AGG | 0.544420 | 4.1:-64011433 | MS.gene72510:CDS |
| TGATGAGTTAGGCACACTGT+TGG | 0.554252 | 4.1:-64012615 | MS.gene72510:CDS |
| CAAATGACATTCTTCAAGCT+AGG | 0.557041 | 4.1:-64018538 | MS.gene72510:CDS |
| GGCTGAGCTTTATGAATCTG+AGG | 0.561921 | 4.1:-64016109 | MS.gene72510:CDS |
| AGCTGACGCGACACTACTAA+AGG | 0.562961 | 4.1:+64017972 | None:intergenic |
| CAAGAATGATTTATGAGGAT+AGG | 0.564825 | 4.1:-64012165 | MS.gene72510:CDS |
| GATCTATCCCATACTGCAGA+AGG | 0.565134 | 4.1:-64013069 | MS.gene72510:intron |
| ATATTACAGGGAAATTAATG+AGG | 0.565776 | 4.1:-64013294 | MS.gene72510:intron |
| AATTATGTACCTTGGGAGGT+GGG | 0.571748 | 4.1:+64012588 | None:intergenic |
| GTCAATACCACTGAGCATCT+GGG | 0.574024 | 4.1:+64016068 | None:intergenic |
| ATTTATGAGGATAGGATGAA+GGG | 0.574286 | 4.1:-64012157 | MS.gene72510:CDS |
| CTAGGATCTTAGATCTGAAG+AGG | 0.577929 | 4.1:-64015419 | MS.gene72510:CDS |
| GTTACGTGTACAATATTGGC+AGG | 0.588398 | 4.1:-64013242 | MS.gene72510:CDS |
| TGTACAGTGTGGCAAGAACA+CGG | 0.592521 | 4.1:+64013201 | None:intergenic |
| CACTACTAAAGGAACATCAT+CGG | 0.592738 | 4.1:+64017983 | None:intergenic |
| TGCCAATATTGTACACGTAA+CGG | 0.598388 | 4.1:+64013244 | None:intergenic |
| TTTATGAATCTGAGGAGCAA+TGG | 0.600846 | 4.1:-64016101 | MS.gene72510:CDS |
| TTGACCTAGATTCAGGAATG+AGG | 0.609769 | 4.1:-64016050 | MS.gene72510:intron |
| GTTGGGAATCCCACCTCCCA+AGG | 0.613288 | 4.1:-64012597 | MS.gene72510:intron |
| GTACAATATTGGCAGGTGCA+GGG | 0.613304 | 4.1:-64013235 | MS.gene72510:CDS |
| GATTATGCTCAATCATAGCT+CGG | 0.614554 | 4.1:+64012780 | None:intergenic |
| AATTGAGAAAAGGCAAATAG+GGG | 0.620222 | 4.1:-64015355 | MS.gene72510:CDS |
| AGATATGTCATAATATCGCA+AGG | 0.635346 | 4.1:+64015380 | None:intergenic |
| AATCTTTACCTTGTACAGTG+TGG | 0.647262 | 4.1:+64013190 | None:intergenic |
| ATGAAATTATGTACCTTGGG+AGG | 0.648793 | 4.1:+64012584 | None:intergenic |
| GTACAGTGTGGCAAGAACAC+GGG | 0.653612 | 4.1:+64013202 | None:intergenic |
| GCAAGAACACGGGAACGTTG+AGG | 0.664537 | 4.1:+64013212 | None:intergenic |
| AAAGCTGCCCAGATGCTCAG+TGG | 0.700066 | 4.1:-64016075 | MS.gene72510:CDS |
| TGATGTGAAACAGTGATGGA+GGG | 0.716084 | 4.1:-64018644 | None:intergenic |
| TCTTCAAAAGACACAACTCG+AGG | 0.728488 | 4.1:+64017864 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 64011413 | 64018651 | 64011413 | ID=MS.gene72510 |
| chr4.1 | mRNA | 64011413 | 64018651 | 64011413 | ID=MS.gene72510.t1;Parent=MS.gene72510 |
| chr4.1 | exon | 64018522 | 64018651 | 64018522 | ID=MS.gene72510.t1.exon1;Parent=MS.gene72510.t1 |
| chr4.1 | CDS | 64018522 | 64018651 | 64018522 | ID=cds.MS.gene72510.t1;Parent=MS.gene72510.t1 |
| chr4.1 | exon | 64017860 | 64018011 | 64017860 | ID=MS.gene72510.t1.exon2;Parent=MS.gene72510.t1 |
| chr4.1 | CDS | 64017860 | 64018011 | 64017860 | ID=cds.MS.gene72510.t1;Parent=MS.gene72510.t1 |
| chr4.1 | exon | 64016051 | 64016154 | 64016051 | ID=MS.gene72510.t1.exon3;Parent=MS.gene72510.t1 |
| chr4.1 | CDS | 64016051 | 64016154 | 64016051 | ID=cds.MS.gene72510.t1;Parent=MS.gene72510.t1 |
| chr4.1 | exon | 64015880 | 64015940 | 64015880 | ID=MS.gene72510.t1.exon4;Parent=MS.gene72510.t1 |
| chr4.1 | CDS | 64015880 | 64015940 | 64015880 | ID=cds.MS.gene72510.t1;Parent=MS.gene72510.t1 |
| chr4.1 | exon | 64015548 | 64015640 | 64015548 | ID=MS.gene72510.t1.exon5;Parent=MS.gene72510.t1 |
| chr4.1 | CDS | 64015548 | 64015640 | 64015548 | ID=cds.MS.gene72510.t1;Parent=MS.gene72510.t1 |
| chr4.1 | exon | 64015351 | 64015451 | 64015351 | ID=MS.gene72510.t1.exon6;Parent=MS.gene72510.t1 |
| chr4.1 | CDS | 64015351 | 64015451 | 64015351 | ID=cds.MS.gene72510.t1;Parent=MS.gene72510.t1 |
| chr4.1 | exon | 64013199 | 64013307 | 64013199 | ID=MS.gene72510.t1.exon7;Parent=MS.gene72510.t1 |
| chr4.1 | CDS | 64013199 | 64013307 | 64013199 | ID=cds.MS.gene72510.t1;Parent=MS.gene72510.t1 |
| chr4.1 | exon | 64013070 | 64013114 | 64013070 | ID=MS.gene72510.t1.exon8;Parent=MS.gene72510.t1 |
| chr4.1 | CDS | 64013070 | 64013114 | 64013070 | ID=cds.MS.gene72510.t1;Parent=MS.gene72510.t1 |
| chr4.1 | exon | 64012914 | 64012982 | 64012914 | ID=MS.gene72510.t1.exon9;Parent=MS.gene72510.t1 |
| chr4.1 | CDS | 64012914 | 64012982 | 64012914 | ID=cds.MS.gene72510.t1;Parent=MS.gene72510.t1 |
| chr4.1 | exon | 64012746 | 64012837 | 64012746 | ID=MS.gene72510.t1.exon10;Parent=MS.gene72510.t1 |
| chr4.1 | CDS | 64012746 | 64012837 | 64012746 | ID=cds.MS.gene72510.t1;Parent=MS.gene72510.t1 |
| chr4.1 | exon | 64012598 | 64012640 | 64012598 | ID=MS.gene72510.t1.exon11;Parent=MS.gene72510.t1 |
| chr4.1 | CDS | 64012598 | 64012640 | 64012598 | ID=cds.MS.gene72510.t1;Parent=MS.gene72510.t1 |
| chr4.1 | exon | 64012144 | 64012203 | 64012144 | ID=MS.gene72510.t1.exon12;Parent=MS.gene72510.t1 |
| chr4.1 | CDS | 64012144 | 64012203 | 64012144 | ID=cds.MS.gene72510.t1;Parent=MS.gene72510.t1 |
| chr4.1 | exon | 64011975 | 64012034 | 64011975 | ID=MS.gene72510.t1.exon13;Parent=MS.gene72510.t1 |
| chr4.1 | CDS | 64011975 | 64012034 | 64011975 | ID=cds.MS.gene72510.t1;Parent=MS.gene72510.t1 |
| chr4.1 | exon | 64011413 | 64011487 | 64011413 | ID=MS.gene72510.t1.exon14;Parent=MS.gene72510.t1 |
| chr4.1 | CDS | 64011413 | 64011487 | 64011413 | ID=cds.MS.gene72510.t1;Parent=MS.gene72510.t1 |
| Gene Sequence |
| Protein sequence |
