AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74006

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74006.t1 MTR_5g058920 98.503 334 5 0 37 370 109 442 0.0 691
MS.gene74006.t1 MTR_5g058920 78.723 47 6 1 1 43 1 47 7.38e-13 69.7
MS.gene74006.t1 MTR_3g072650 62.587 433 96 6 1 370 1 430 5.76e-170 481
MS.gene74006.t1 MTR_3g072650 60.789 380 83 6 1 317 1 377 1.54e-152 436
MS.gene74006.t1 MTR_7g451440 60.938 256 76 3 94 335 168 413 1.21e-102 310
MS.gene74006.t1 MTR_4g099180 58.462 260 91 4 86 345 140 382 1.11e-98 299
MS.gene74006.t1 MTR_8g007500 60.938 256 85 2 119 361 191 444 2.51e-92 284
MS.gene74006.t1 MTR_4g099180 63.462 156 50 2 86 241 140 288 2.65e-62 202
MS.gene74006.t1 MTR_4g099180 37.600 125 78 0 163 287 164 288 1.20e-17 83.2
MS.gene74006.t1 MTR_4g099180 63.871 155 49 2 86 240 140 287 3.14e-62 202
MS.gene74006.t1 MTR_4g099180 37.903 124 77 0 163 286 164 287 1.44e-17 82.8
MS.gene74006.t1 MTR_8g080860 41.436 181 106 0 119 299 14 194 1.25e-37 137
MS.gene74006.t1 MTR_8g080860 37.912 182 106 1 101 282 95 269 7.58e-32 122
MS.gene74006.t1 MTR_8g080860 41.436 181 106 0 119 299 10 190 1.33e-37 137
MS.gene74006.t1 MTR_8g080860 37.912 182 106 1 101 282 91 265 7.54e-32 121
MS.gene74006.t1 MTR_8g092090 36.842 209 101 1 123 300 7 215 9.90e-31 117
MS.gene74006.t1 MTR_8g092090 37.179 156 98 0 116 271 77 232 6.25e-25 102
MS.gene74006.t1 MTR_8g088930 36.313 179 114 0 120 298 8 186 6.23e-29 118
MS.gene74006.t1 MTR_8g088930 36.090 133 85 0 168 300 10 142 2.71e-17 84.0
MS.gene74006.t1 MTR_8g088930 42.708 96 55 0 118 213 98 193 4.83e-14 73.9
MS.gene74006.t1 MTR_8g088930 37.719 114 71 0 125 238 82 195 6.10e-13 70.5
MS.gene74006.t1 MTR_8g088930 36.313 179 114 0 120 298 8 186 6.24e-29 118
MS.gene74006.t1 MTR_8g088930 36.090 133 85 0 168 300 10 142 2.69e-17 84.0
MS.gene74006.t1 MTR_8g088930 42.708 96 55 0 118 213 98 193 4.88e-14 73.9
MS.gene74006.t1 MTR_8g088930 37.719 114 71 0 125 238 82 195 6.21e-13 70.5
MS.gene74006.t1 MTR_8g088930 31.020 245 164 3 120 359 8 252 6.31e-29 118
MS.gene74006.t1 MTR_8g088930 42.708 96 55 0 118 213 98 193 5.01e-14 73.9
MS.gene74006.t1 MTR_8g088930 31.020 245 164 3 120 359 8 252 6.36e-29 118
MS.gene74006.t1 MTR_8g088930 42.708 96 55 0 118 213 98 193 4.87e-14 73.9
MS.gene74006.t1 MTR_8g095240 34.211 228 109 4 102 295 34 254 1.05e-26 110
MS.gene74006.t1 MTR_8g095240 37.563 197 106 4 124 303 118 314 1.46e-26 110
MS.gene74006.t1 MTR_8g095240 34.857 175 111 2 116 287 166 340 7.07e-22 96.7
MS.gene74006.t1 MTR_8g095240 32.258 186 116 1 123 298 2 187 4.25e-19 88.6
MS.gene74006.t1 MTR_8g095240 28.283 198 132 1 110 297 12 209 1.29e-16 81.3
MS.gene74006.t1 MTR_8g095240 37.600 125 78 0 176 300 9 133 1.78e-15 77.8
MS.gene74006.t1 MTR_8g095240 34.884 129 82 1 115 241 212 340 1.63e-13 71.6
MS.gene74006.t1 MTR_5g459450 38.650 163 100 0 126 288 70 232 5.41e-26 110
MS.gene74006.t1 MTR_5g459450 38.889 126 77 0 173 298 71 196 6.22e-19 89.0
MS.gene74006.t1 MTR_4g107460 38.095 168 104 0 130 297 61 228 9.89e-26 106
MS.gene74006.t1 MTR_4g107460 35.122 205 109 2 118 298 2 206 6.49e-24 101
MS.gene74006.t1 MTR_4g107460 31.720 186 124 1 110 292 87 272 1.20e-20 92.0
MS.gene74006.t1 MTR_4g107460 34.586 133 82 2 171 300 9 139 2.72e-13 70.5
MS.gene74006.t1 MTR_3g073100 37.778 180 111 1 126 304 58 237 1.57e-24 105
MS.gene74006.t1 MTR_3g073100 34.014 147 97 0 124 270 80 226 2.40e-16 80.9
MS.gene74006.t1 MTR_7g089950 31.746 189 129 0 105 293 51 239 2.10e-24 105
MS.gene74006.t1 MTR_8g039100 35.057 174 113 0 122 295 50 223 3.46e-24 104
MS.gene74006.t1 MTR_8g039100 40.449 89 53 0 115 203 135 223 1.36e-13 72.8
MS.gene74006.t1 MTR_8g039100 31.081 148 102 0 124 271 98 245 1.84e-12 68.9
MS.gene74006.t1 MTR_8g080310 39.850 133 80 0 167 299 6 138 1.53e-23 95.9
MS.gene74006.t1 MTR_8g080310 34.839 155 101 0 118 272 3 157 3.20e-22 92.4
MS.gene74006.t1 MTR_8g080310 36.842 133 61 1 115 224 23 155 1.19e-14 71.2
MS.gene74006.t1 MTR_1g050740 34.706 170 111 0 126 295 62 231 1.84e-23 102
MS.gene74006.t1 MTR_1g050740 38.281 128 79 0 173 300 63 190 1.67e-19 90.5
MS.gene74006.t1 MTR_1g050740 31.977 172 117 0 123 294 82 253 2.96e-18 87.0
MS.gene74006.t1 MTR_3g097260 37.647 170 106 0 126 295 56 225 2.97e-22 99.0
MS.gene74006.t1 MTR_3g097260 33.077 130 87 0 124 253 100 229 4.03e-12 68.2
MS.gene74006.t1 MTR_8g080340 37.107 159 76 2 124 282 19 153 2.53e-21 90.1
MS.gene74006.t1 MTR_8g080340 38.400 125 77 0 112 236 29 153 3.63e-21 89.7
MS.gene74006.t1 MTR_8g080340 32.800 125 84 0 176 300 24 148 2.43e-16 76.3
MS.gene74006.t1 MTR_8g080340 37.624 101 63 0 194 294 19 119 7.26e-15 72.4
MS.gene74006.t1 MTR_8g095170 35.294 170 103 2 126 294 31 194 6.92e-21 94.7
MS.gene74006.t1 MTR_8g095170 36.522 115 73 0 186 300 46 160 8.97e-16 79.3
MS.gene74006.t1 MTR_8g095170 35.294 170 103 2 126 294 31 194 1.24e-20 94.0
MS.gene74006.t1 MTR_8g095170 36.522 115 73 0 186 300 46 160 1.24e-15 78.6
MS.gene74006.t1 MTR_1g062740 31.609 174 119 0 110 283 59 232 4.23e-20 92.4
MS.gene74006.t1 MTR_1g062740 33.161 193 126 2 126 316 52 243 1.00e-19 91.3
MS.gene74006.t1 MTR_1g062740 33.333 138 69 2 124 238 96 233 3.09e-15 77.8
MS.gene74006.t1 MTR_1g062740 34.091 132 87 0 169 300 49 180 2.75e-13 71.6
MS.gene74006.t1 MTR_3g014650 32.941 170 102 1 126 295 90 247 1.61e-19 90.9
MS.gene74006.t1 MTR_3g014650 41.573 89 52 0 115 203 159 247 2.54e-13 71.6
MS.gene74006.t1 MTR_3g014650 32.609 138 93 0 124 261 122 259 6.24e-13 70.5
MS.gene74006.t1 MTR_3g014650 36.607 112 65 1 193 298 93 204 4.93e-12 67.8
MS.gene74006.t1 MTR_6g043150 33.161 193 106 1 125 294 79 271 8.13e-18 83.6
MS.gene74006.t1 MTR_6g043150 32.768 177 119 0 119 295 4 180 3.77e-16 79.0
MS.gene74006.t1 MTR_6g043150 32.639 144 96 1 167 310 6 148 3.63e-15 75.9
MS.gene74006.t1 MTR_6g043150 34.637 179 94 2 124 279 147 325 4.02e-15 75.9
MS.gene74006.t1 MTR_8g080350 41.525 118 67 1 153 270 87 202 2.31e-17 83.2
MS.gene74006.t1 MTR_8g080350 26.724 232 112 6 115 298 116 337 3.13e-12 67.8
MS.gene74006.t1 MTR_8g080350 30.769 182 114 3 97 277 169 339 4.77e-11 63.9
MS.gene74006.t1 MTR_2g078470 31.443 194 126 1 107 300 68 254 1.98e-16 80.1
MS.gene74006.t1 MTR_2g078470 33.918 171 113 0 124 294 170 340 2.58e-16 79.7
MS.gene74006.t1 MTR_2g078470 33.333 165 110 0 124 288 32 196 4.32e-15 76.3
MS.gene74006.t1 MTR_2g078470 28.571 210 145 2 124 329 124 332 1.39e-13 71.6
MS.gene74006.t1 MTR_2g078470 34.722 144 94 0 125 268 217 360 8.59e-13 69.3
MS.gene74006.t1 MTR_5g010840 32.022 178 112 2 125 302 181 349 4.37e-15 76.3
MS.gene74006.t1 MTR_5g010840 29.384 211 111 3 123 295 110 320 2.97e-14 73.9
MS.gene74006.t1 MTR_5g010840 34.694 147 96 0 124 270 218 364 3.99e-13 70.5
MS.gene74006.t1 MTR_8g092410 35.036 137 84 3 157 288 140 276 5.17e-15 76.3
MS.gene74006.t1 MTR_8g092410 32.593 135 80 3 166 297 136 262 5.56e-13 70.1
MS.gene74006.t1 MTR_8g092410 33.094 139 79 3 125 253 179 313 9.43e-11 63.2
MS.gene74006.t1 MTR_3g023310 41.237 97 47 1 209 295 11 107 3.80e-13 67.4
MS.gene74006.t1 MTR_3g023310 35.345 116 65 2 110 215 4 119 1.26e-11 63.2
MS.gene74006.t1 MTR_3g023310 32.540 126 72 1 164 289 12 124 1.68e-11 62.8
MS.gene74006.t1 MTR_1g112280 30.657 137 95 0 167 303 609 745 6.83e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74006.t1 AT1G21920 68.598 328 90 4 40 366 100 415 6.05e-149 427
MS.gene74006.t1 AT1G77660 65.046 329 107 2 40 368 101 421 3.27e-140 405
MS.gene74006.t1 AT1G21920 68.459 279 75 4 40 317 100 366 6.45e-137 395
MS.gene74006.t1 AT4G17080 52.761 326 132 3 45 360 189 502 1.95e-101 309
MS.gene74006.t1 AT2G35170 51.875 320 118 7 44 351 147 442 2.20e-98 300
MS.gene74006.t1 AT1G21980 37.968 187 115 1 119 304 69 255 1.56e-29 120
MS.gene74006.t1 AT1G10900 38.286 175 108 0 124 298 10 184 1.97e-27 114
MS.gene74006.t1 AT1G10900 36.691 139 65 2 123 238 55 193 2.01e-13 72.0
MS.gene74006.t1 AT1G10900 38.286 175 108 0 124 298 10 184 1.97e-27 114
MS.gene74006.t1 AT1G10900 36.691 139 65 2 123 238 55 193 2.01e-13 72.0
MS.gene74006.t1 AT1G10900 38.286 175 108 0 124 298 10 184 1.97e-27 114
MS.gene74006.t1 AT1G10900 36.691 139 65 2 123 238 55 193 2.01e-13 72.0
MS.gene74006.t1 AT1G60890 36.000 175 112 0 124 298 10 184 2.57e-27 114
MS.gene74006.t1 AT1G60890 33.908 174 115 0 110 283 19 192 5.86e-23 101
MS.gene74006.t1 AT1G60890 35.115 131 85 0 170 300 10 140 2.33e-16 81.3
MS.gene74006.t1 AT1G60890 35.252 139 67 2 123 238 55 193 3.70e-13 71.2
MS.gene74006.t1 AT1G60890 36.000 175 112 0 124 298 22 196 3.33e-27 113
MS.gene74006.t1 AT1G60890 33.908 174 115 0 110 283 31 204 7.66e-23 100
MS.gene74006.t1 AT1G60890 35.115 131 85 0 170 300 22 152 3.05e-16 80.9
MS.gene74006.t1 AT1G60890 35.252 139 67 2 123 238 67 205 4.66e-13 70.9
MS.gene74006.t1 AT1G77740 38.547 179 109 1 119 296 70 248 5.82e-27 112
MS.gene74006.t1 AT1G77740 38.095 126 78 0 173 298 79 204 1.36e-17 84.7
MS.gene74006.t1 AT1G77740 38.547 179 109 1 119 296 70 248 5.82e-27 112
MS.gene74006.t1 AT1G77740 38.095 126 78 0 173 298 79 204 1.36e-17 84.7
MS.gene74006.t1 AT2G41210 36.111 180 109 1 122 295 61 240 3.93e-26 110
MS.gene74006.t1 AT2G41210 35.165 182 117 1 123 304 91 271 8.24e-24 103
MS.gene74006.t1 AT2G41210 38.281 128 79 0 173 300 72 199 3.58e-20 92.8
MS.gene74006.t1 AT3G07960 35.882 170 109 0 126 295 28 197 2.98e-25 107
MS.gene74006.t1 AT3G07960 32.298 161 109 0 123 283 48 208 1.79e-20 93.6
MS.gene74006.t1 AT3G07960 36.508 126 80 0 173 298 29 154 6.99e-18 85.5
MS.gene74006.t1 AT3G07960 35.882 170 109 0 126 295 28 197 2.98e-25 107
MS.gene74006.t1 AT3G07960 32.298 161 109 0 123 283 48 208 1.79e-20 93.6
MS.gene74006.t1 AT3G07960 36.508 126 80 0 173 298 29 154 6.99e-18 85.5
MS.gene74006.t1 AT3G07960 35.882 170 109 0 126 295 28 197 3.28e-25 107
MS.gene74006.t1 AT3G07960 32.298 161 109 0 123 283 48 208 1.69e-20 93.6
MS.gene74006.t1 AT3G07960 36.508 126 80 0 173 298 29 154 7.31e-18 85.5
MS.gene74006.t1 AT3G07960 35.882 170 109 0 126 295 28 197 3.73e-25 107
MS.gene74006.t1 AT3G07960 32.298 161 109 0 123 283 48 208 2.03e-20 93.6
MS.gene74006.t1 AT3G07960 36.508 126 80 0 173 298 29 154 7.04e-18 85.9
MS.gene74006.t1 AT3G56960 33.529 170 113 0 126 295 73 242 1.45e-24 105
MS.gene74006.t1 AT3G56960 32.967 182 121 1 123 304 93 273 1.52e-22 99.8
MS.gene74006.t1 AT3G56960 36.719 128 81 0 173 300 74 201 1.32e-19 90.9
MS.gene74006.t1 AT3G56960 33.529 170 113 0 126 295 73 242 1.45e-24 105
MS.gene74006.t1 AT3G56960 32.967 182 121 1 123 304 93 273 1.52e-22 99.8
MS.gene74006.t1 AT3G56960 36.719 128 81 0 173 300 74 201 1.32e-19 90.9
MS.gene74006.t1 AT2G26420 36.250 160 102 0 126 285 54 213 9.97e-23 100
MS.gene74006.t1 AT2G26420 36.508 126 80 0 173 298 55 180 1.23e-16 82.0
MS.gene74006.t1 AT2G26420 35.333 150 97 0 123 272 74 223 4.25e-16 80.5
MS.gene74006.t1 AT2G26420 36.250 160 102 0 126 285 54 213 1.17e-22 100
MS.gene74006.t1 AT2G26420 36.508 126 80 0 173 298 55 180 1.40e-16 81.6
MS.gene74006.t1 AT2G26420 35.333 150 97 0 123 272 74 223 4.78e-16 80.1
MS.gene74006.t1 AT2G26420 36.250 160 102 0 126 285 54 213 1.17e-22 100
MS.gene74006.t1 AT2G26420 36.508 126 80 0 173 298 55 180 1.40e-16 81.6
MS.gene74006.t1 AT2G26420 35.333 150 97 0 123 272 74 223 4.78e-16 80.1
MS.gene74006.t1 AT3G09920 31.980 197 130 1 102 298 34 226 8.75e-21 94.7
MS.gene74006.t1 AT3G09920 31.609 174 119 0 110 283 61 234 2.64e-20 93.2
MS.gene74006.t1 AT3G09920 34.694 147 73 2 115 238 89 235 8.22e-16 79.7
MS.gene74006.t1 AT3G09920 34.375 128 84 0 173 300 55 182 1.60e-13 72.4
MS.gene74006.t1 AT3G09920 31.980 197 130 1 102 298 34 226 8.75e-21 94.7
MS.gene74006.t1 AT3G09920 31.609 174 119 0 110 283 61 234 2.64e-20 93.2
MS.gene74006.t1 AT3G09920 34.694 147 73 2 115 238 89 235 8.22e-16 79.7
MS.gene74006.t1 AT3G09920 34.375 128 84 0 173 300 55 182 1.60e-13 72.4
MS.gene74006.t1 AT3G09920 31.980 197 130 1 102 298 34 226 8.75e-21 94.7
MS.gene74006.t1 AT3G09920 31.609 174 119 0 110 283 61 234 2.64e-20 93.2
MS.gene74006.t1 AT3G09920 34.694 147 73 2 115 238 89 235 8.22e-16 79.7
MS.gene74006.t1 AT3G09920 34.375 128 84 0 173 300 55 182 1.60e-13 72.4
MS.gene74006.t1 AT3G09920 31.980 197 130 1 102 298 34 226 8.75e-21 94.7
MS.gene74006.t1 AT3G09920 31.609 174 119 0 110 283 61 234 2.64e-20 93.2
MS.gene74006.t1 AT3G09920 34.694 147 73 2 115 238 89 235 8.22e-16 79.7
MS.gene74006.t1 AT3G09920 34.375 128 84 0 173 300 55 182 1.60e-13 72.4

Find 101 sgRNAs with CRISPR-Local

Find 213 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCCACTTGGTCATCATTTC+GGG 0.092585 5.3:+46626322 None:intergenic
TATACTGGTGATTGTTATTC+TGG 0.140108 5.3:-46628256 MS.gene74006:CDS
TCTCCACTTGGTCATCATTT+CGG 0.221085 5.3:+46626321 None:intergenic
AAATATACAGGGGAGTATTT+TGG 0.233941 5.3:-46627191 MS.gene74006:CDS
TATTCTGGGGAATGGTTTAA+TGG 0.242057 5.3:-46628241 MS.gene74006:CDS
TTCGTTCTCCTCTGTTGAAC+CGG 0.268055 5.3:+46628534 None:intergenic
AACAGTGTCCATTCGCAAAA+TGG 0.316812 5.3:+46627141 None:intergenic
GGAGACAAAATGCATGGATT+TGG 0.331061 5.3:-46627170 MS.gene74006:CDS
ACACCGAAGCAAAGAGGATT+TGG 0.332044 5.3:+46629023 None:intergenic
CCATTACTGCTTTCTCCACT+TGG 0.336002 5.3:+46626309 None:intergenic
TGCAGCAGTGAAAGCTGTTC+AGG 0.366394 5.3:-46626255 MS.gene74006:CDS
AATGGACAAAGTCATGGTTT+TGG 0.371589 5.3:-46628223 MS.gene74006:CDS
TATGTTGGTGAATTTAAGTT+TGG 0.373929 5.3:-46628172 MS.gene74006:CDS
TATGAAGGTGATTGGGTTGA+TGG 0.387527 5.3:-46628379 MS.gene74006:CDS
AAGCAAAGAGGATTTGGTTC+GGG 0.413038 5.3:+46629029 None:intergenic
AAGCAGTAATGGCAGCAAAC+AGG 0.414541 5.3:-46626298 MS.gene74006:CDS
GGTGTTTATACATTTCGAAA+TGG 0.427912 5.3:-46627080 MS.gene74006:CDS
AGGAATGGAGATAAATATAC+AGG 0.428752 5.3:-46627203 MS.gene74006:CDS
GATGGAAAATATGATGGTTA+TGG 0.429760 5.3:-46628361 MS.gene74006:CDS
ATGGAATTGAAAGCTGGGCT+AGG 0.431240 5.3:-46628342 MS.gene74006:CDS
CTTGGTCATCATTTCGGGGA+TGG 0.432258 5.3:+46626327 None:intergenic
AAACCGGTTCAATGGTTCAT+AGG 0.432685 5.3:-46628562 MS.gene74006:CDS
TTTGGTTCTAAACATGGTCT+TGG 0.434793 5.3:-46628154 MS.gene74006:CDS
GAAGGTCGTCGACAAGGTTA+TGG 0.435450 5.3:-46627101 MS.gene74006:CDS
GTGGAAGATATGAAGGTGAT+TGG 0.439954 5.3:-46628387 MS.gene74006:CDS
GAGGCTTGGAGTGGAGGATC+GGG 0.441401 5.3:+46628987 None:intergenic
TTCATGGTGGAAAGTGTAAT+GGG 0.447066 5.3:-46628435 MS.gene74006:CDS
TTCTACGAAGGAGAATTTCA+TGG 0.448504 5.3:-46628451 MS.gene74006:CDS
ATACTGGTGATTGTTATTCT+GGG 0.456453 5.3:-46628255 MS.gene74006:CDS
TGTCCCATTCACCACATCTT+CGG 0.459760 5.3:+46627054 None:intergenic
GAAAGCTGTTCAGGATAGAA+TGG 0.466671 5.3:-46626246 MS.gene74006:CDS
GAACCAAATCCTCTTTGCTT+CGG 0.466878 5.3:-46629026 MS.gene74006:CDS
TGGAATTGAAAGCTGGGCTA+GGG 0.468838 5.3:-46628341 MS.gene74006:CDS
GAAGCAAAGAGGATTTGGTT+CGG 0.470488 5.3:+46629028 None:intergenic
TCGTTCTCCTCTGTTGAACC+GGG 0.470876 5.3:+46628535 None:intergenic
TGGTTCGGGTGAGTTTAGTT+TGG 0.471218 5.3:+46629043 None:intergenic
TTTCATGGTGGAAAGTGTAA+TGG 0.473474 5.3:-46628436 MS.gene74006:CDS
GCAAGGACAGCCTGCGTTTG+TGG 0.474259 5.3:+46627005 None:intergenic
GCGAATGGACACTGTTATGA+AGG 0.474775 5.3:-46627134 MS.gene74006:CDS
CAAGGTTTGAGACATGGTTA+TGG 0.475027 5.3:-46628292 MS.gene74006:CDS
GTGATTGTTATTCTGGGGAA+TGG 0.480781 5.3:-46628249 MS.gene74006:CDS
TCACCTATGAACCATTGAAC+CGG 0.484374 5.3:+46628559 None:intergenic
TGGTTAAATTGCAGGCTGCT+AGG 0.485769 5.3:-46626373 MS.gene74006:intron
TGAGGCTTGGAGTGGAGGAT+CGG 0.486167 5.3:+46628986 None:intergenic
TCTGATGGAAGTTGTTATGT+TGG 0.486647 5.3:-46628187 MS.gene74006:CDS
GGCTGTCCTTGCATCAGCTC+AGG 0.494994 5.3:-46626994 MS.gene74006:intron
CCTAGGCTCGGAATGAGGCT+TGG 0.497798 5.3:+46628973 None:intergenic
TGGAAGATATGAAGGTGATT+GGG 0.511435 5.3:-46628386 MS.gene74006:CDS
GGTGTTCAATCTTGTTCTGA+TGG 0.512152 5.3:-46628202 MS.gene74006:CDS
TTTGTGAGTGGAAGATATGA+AGG 0.513634 5.3:-46628394 MS.gene74006:CDS
GGAGTGGAGGATCGGGTGGT+TGG 0.518620 5.3:+46628994 None:intergenic
CTCTCTACCGCCACAAACGC+AGG 0.521164 5.3:-46627015 MS.gene74006:CDS
ATGGTTATGGAATTGAAAGC+TGG 0.525844 5.3:-46628348 MS.gene74006:CDS
CCAAGTGGAGAAAGCAGTAA+TGG 0.535060 5.3:-46626309 MS.gene74006:CDS
CGTGAAAGGGTTGTTAAAAG+AGG 0.538810 5.3:-46628506 MS.gene74006:CDS
TATAAGGGTTGTTATAAACA+AGG 0.540310 5.3:-46628310 MS.gene74006:CDS
CCAAGCCTCATTCCGAGCCT+AGG 0.540642 5.3:-46628973 MS.gene74006:intron
TTTAAGTTTGGTTCTAAACA+TGG 0.544906 5.3:-46628160 MS.gene74006:CDS
TGGTTTAATGGACAAAGTCA+TGG 0.545446 5.3:-46628229 MS.gene74006:CDS
TGGGTTGATGGAAAATATGA+TGG 0.547236 5.3:-46628367 MS.gene74006:CDS
CGGAATGAGGCTTGGAGTGG+AGG 0.549270 5.3:+46628981 None:intergenic
TACTGGTGATTGTTATTCTG+GGG 0.555101 5.3:-46628254 MS.gene74006:CDS
TGCTGCAACTCTAGCAGCAG+TGG 0.555209 5.3:+46626271 None:intergenic
AGCAGTAATGGCAGCAAACA+GGG 0.556769 5.3:-46626297 MS.gene74006:CDS
AGAACGAAAAGAACGTGAAA+GGG 0.559441 5.3:-46628519 MS.gene74006:CDS
CAATGGTTCATAGGTGAACC+CGG 0.559788 5.3:-46628553 MS.gene74006:CDS
GAGACCGAAGATGTGGTGAA+TGG 0.560592 5.3:-46627058 MS.gene74006:CDS
AAAGCTGTTCAGGATAGAAT+GGG 0.560882 5.3:-46626245 MS.gene74006:CDS
GAGAACGAAAAGAACGTGAA+AGG 0.566461 5.3:-46628520 MS.gene74006:CDS
CGGAACCTAGGCTCGGAATG+AGG 0.567359 5.3:+46628968 None:intergenic
GGTGGAAAGTGTAATGGGAG+TGG 0.567603 5.3:-46628430 MS.gene74006:CDS
TGCAACTCTAGCAGCAGTGG+CGG 0.573289 5.3:+46626274 None:intergenic
GACGGGAATTTAAAACGACT+CGG 0.577658 5.3:-46628607 MS.gene74006:CDS
TCCACTTGGTCATCATTTCG+GGG 0.578987 5.3:+46626323 None:intergenic
AGACCGAAGATGTGGTGAAT+GGG 0.583099 5.3:-46627057 MS.gene74006:CDS
GTGAAAGGGTTGTTAAAAGA+GGG 0.590860 5.3:-46628505 MS.gene74006:CDS
GGAATTGAAAGCTGGGCTAG+GGG 0.591885 5.3:-46628340 MS.gene74006:CDS
GCTTGGAGTGGAGGATCGGG+TGG 0.594218 5.3:+46628990 None:intergenic
CGAAATGGAGACCGAAGATG+TGG 0.602119 5.3:-46627065 MS.gene74006:CDS
GACACTGTTATGAAGGAGCA+TGG 0.603134 5.3:-46627127 MS.gene74006:CDS
GAAGTTGAATTCTACAGCAA+TGG 0.609398 5.3:-46628478 MS.gene74006:CDS
AGGACAGCCTGCGTTTGTGG+CGG 0.609546 5.3:+46627008 None:intergenic
TCGTTGACGGAACCTAGGCT+CGG 0.610315 5.3:+46628961 None:intergenic
GTGGCGGTAGAGAGTGCTTG+AGG 0.612938 5.3:+46627024 None:intergenic
GTTTATGAAATAAAGCGCTA+CGG 0.614497 5.3:+46628681 None:intergenic
GCTCGGAATGAGGCTTGGAG+TGG 0.616498 5.3:+46628978 None:intergenic
TATGAAGGAGCATGGCATGA+AGG 0.616571 5.3:-46627119 MS.gene74006:CDS
TATAAACAAGGTTTGAGACA+TGG 0.620037 5.3:-46628298 MS.gene74006:CDS
TGGCATGAAGGTCGTCGACA+AGG 0.621349 5.3:-46627107 MS.gene74006:CDS
AGCAATGGTGATTTCTACGA+AGG 0.631450 5.3:-46628463 MS.gene74006:CDS
TGGTTATGGAATTGAAAGCT+GGG 0.641123 5.3:-46628347 MS.gene74006:CDS
GAATTGAAAGCTGGGCTAGG+GGG 0.641129 5.3:-46628339 MS.gene74006:CDS
CAAATACCTGAGCTGATGCA+AGG 0.643584 5.3:+46626988 None:intergenic
GGAATGGAGATAAATATACA+GGG 0.645709 5.3:-46627202 MS.gene74006:CDS
GCTGTTCAGGATAGAATGGG+TGG 0.647734 5.3:-46626242 MS.gene74006:CDS
TCCCCGAAATGATGACCAAG+TGG 0.654313 5.3:-46626324 MS.gene74006:CDS
AGAAGAACACCGAAGCAAAG+AGG 0.656694 5.3:+46629017 None:intergenic
AGGTGAACCCGGTTCAACAG+AGG 0.658057 5.3:-46628542 MS.gene74006:CDS
TACGAAGGAGAATTTCATGG+TGG 0.674011 5.3:-46628448 MS.gene74006:CDS
GAATGGAGATAAATATACAG+GGG 0.695134 5.3:-46627201 MS.gene74006:CDS
AGATGTGGTGAATGGGACAG+TGG 0.738225 5.3:-46627050 MS.gene74006:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTAAACCATTATTATTATAT+AGG - chr5.3:46627962-46627981 MS.gene74006:intron 15.0%
!! TCACAAATTAATATCTAAAT+CGG - chr5.3:46628373-46628392 MS.gene74006:CDS 15.0%
!!! TCATTTACGTTAATTTTTTT+TGG - chr5.3:46628884-46628903 MS.gene74006:intron 15.0%
!!! TTTTGAATGAGTTTATTTTT+AGG + chr5.3:46628343-46628362 None:intergenic 15.0%
!! AAAAAAAAACTCACCTAAAA+TGG + chr5.3:46627155-46627174 None:intergenic 20.0%
!! AAAAAATACAGAGATCTATT+TGG + chr5.3:46628824-46628843 None:intergenic 20.0%
!! AAAAGTAAGGATCTAAAAAT+AGG + chr5.3:46628788-46628807 None:intergenic 20.0%
!! AAAGTAAGGATCTAAAAATA+GGG + chr5.3:46628787-46628806 None:intergenic 20.0%
!! AGCTTCCTATATAATAATAA+TGG + chr5.3:46627970-46627989 None:intergenic 20.0%
!! TACTACTATTTATAATTCCA+CGG + chr5.3:46628637-46628656 None:intergenic 20.0%
!!! AGTTCATAACATTTTCATAT+CGG + chr5.3:46627432-46627451 None:intergenic 20.0%
!!! ATAAATAGTAGTACCTTTTA+TGG - chr5.3:46628644-46628663 MS.gene74006:CDS 20.0%
!!! ATGAGTTTATTTTTAGGAAA+AGG + chr5.3:46628337-46628356 None:intergenic 20.0%
!!! ATTTTTTTTGGTTAAATTGC+AGG - chr5.3:46628896-46628915 MS.gene74006:intron 20.0%
!!! CTCAAGTTTTTATTGAAATT+GGG - chr5.3:46627185-46627204 MS.gene74006:CDS 20.0%
!!! GTATTCTATTTTTTCCATAT+CGG - chr5.3:46626523-46626542 MS.gene74006:intron 20.0%
!!! TCTCAAGTTTTTATTGAAAT+TGG - chr5.3:46627184-46627203 MS.gene74006:CDS 20.0%
!!! TGAATGTTTTAAAACAGAAA+CGG + chr5.3:46626647-46626666 None:intergenic 20.0%
!!! TTACTAACATTTTCCATAAA+AGG + chr5.3:46628660-46628679 None:intergenic 20.0%
!!! TTCTGTTTTAAAACATTCAT+GGG - chr5.3:46626648-46626667 MS.gene74006:intron 20.0%
!!! TTTCTGTTTTAAAACATTCA+TGG - chr5.3:46626647-46626666 MS.gene74006:intron 20.0%
! AAAATTGTGAAAGACCAAAA+TGG - chr5.3:46627460-46627479 MS.gene74006:intron 25.0%
! AAATTACACCATAGAATTTG+AGG + chr5.3:46627725-46627744 None:intergenic 25.0%
! ACATTGTTTGACAAGTTAAA+AGG - chr5.3:46627821-46627840 MS.gene74006:intron 25.0%
! ACTACTATTTATAATTCCAC+GGG + chr5.3:46628636-46628655 None:intergenic 25.0%
! AGAACGCTCAATAATTTATT+AGG + chr5.3:46628597-46628616 None:intergenic 25.0%
! ATAAGAAAAAGAAGAAAACC+CGG - chr5.3:46626392-46626411 MS.gene74006:intron 25.0%
! ATTGTCAAATGACATTAACA+AGG + chr5.3:46627898-46627917 None:intergenic 25.0%
! CATTGTTTGACAAGTTAAAA+GGG - chr5.3:46627822-46627841 MS.gene74006:intron 25.0%
! TATAAGGGTTGTTATAAACA+AGG - chr5.3:46626967-46626986 MS.gene74006:intron 25.0%
!! AAAATAGGGATATTTTCAAC+AGG + chr5.3:46628773-46628792 None:intergenic 25.0%
!! AATAGAGTCTACTTTATTTG+AGG + chr5.3:46627634-46627653 None:intergenic 25.0%
!! ATTATTGAGCGTTCTTTTAA+TGG - chr5.3:46628602-46628621 MS.gene74006:CDS 25.0%
!! GGTGTTTATAGATTTTATAC+TGG - chr5.3:46627006-46627025 MS.gene74006:CDS 25.0%
!! GTTCATAACATTTTCATATC+GGG + chr5.3:46627431-46627450 None:intergenic 25.0%
!! TATGTTGGTGAATTTAAGTT+TGG - chr5.3:46627105-46627124 MS.gene74006:CDS 25.0%
!! TTGACGATTTATGTTCTTTT+GGG - chr5.3:46627552-46627571 MS.gene74006:intron 25.0%
!! TTTGACGATTTATGTTCTTT+TGG - chr5.3:46627551-46627570 MS.gene74006:intron 25.0%
!!! GCAACAATCATATTTTTGAT+TGG - chr5.3:46628708-46628727 MS.gene74006:CDS 25.0%
!!! GTTTATCATTATTTTGTGAG+TGG - chr5.3:46626871-46626890 MS.gene74006:intron 25.0%
!!! TAGACTCTATTGTTGATTTT+TGG - chr5.3:46627643-46627662 MS.gene74006:intron 25.0%
!!! TTTAAGTTTGGTTCTAAACA+TGG - chr5.3:46627117-46627136 MS.gene74006:CDS 25.0%
!!! TTTGAGGACTATTTTATTTG+AGG + chr5.3:46627618-46627637 None:intergenic 25.0%
AATAGACCTTAGACTATAGT+TGG - chr5.3:46627995-46628014 MS.gene74006:intron 30.0%
ACATAACCAACTATAGTCTA+AGG + chr5.3:46628004-46628023 None:intergenic 30.0%
AGACTGAAAAAACCTAGAAT+CGG + chr5.3:46626484-46626503 None:intergenic 30.0%
AGCAGTAGTAATAATAAACC+GGG + chr5.3:46626458-46626477 None:intergenic 30.0%
AGGAATGGAGATAAATATAC+AGG - chr5.3:46628074-46628093 MS.gene74006:intron 30.0%
CAGCAGTAGTAATAATAAAC+CGG + chr5.3:46626459-46626478 None:intergenic 30.0%
GAATGGAGATAAATATACAG+GGG - chr5.3:46628076-46628095 MS.gene74006:intron 30.0%
GATGGAAAATATGATGGTTA+TGG - chr5.3:46626916-46626935 MS.gene74006:intron 30.0%
GGAATGGAGATAAATATACA+GGG - chr5.3:46628075-46628094 MS.gene74006:intron 30.0%
GGTGTTTATACATTTCGAAA+TGG - chr5.3:46628197-46628216 MS.gene74006:CDS 30.0%
GTAAAAATCTTCTTCGTTGA+CGG + chr5.3:46626332-46626351 None:intergenic 30.0%
TAAAAAACAGCAGACCTAAT+TGG - chr5.3:46628403-46628422 MS.gene74006:CDS 30.0%
TACGCAAACAGAATATCATT+CGG + chr5.3:46628553-46628572 None:intergenic 30.0%
TATGCAAAAGCAATAGTAGT+AGG - chr5.3:46627917-46627936 MS.gene74006:intron 30.0%
TCACTGTAACATGAAAAGTA+AGG + chr5.3:46628801-46628820 None:intergenic 30.0%
TTTATGCCATCTACAAGTTT+CGG - chr5.3:46628426-46628445 MS.gene74006:CDS 30.0%
! ATACTGGTGATTGTTATTCT+GGG - chr5.3:46627022-46627041 MS.gene74006:CDS 30.0%
! ATATGAGTGAAGAACCATTT+TGG + chr5.3:46627477-46627496 None:intergenic 30.0%
! GTTTATGAAATAAAGCGCTA+CGG + chr5.3:46626599-46626618 None:intergenic 30.0%
! TAGAATCAGCAACACATTTT+CGG + chr5.3:46626566-46626585 None:intergenic 30.0%
! TATAAACAAGGTTTGAGACA+TGG - chr5.3:46626979-46626998 MS.gene74006:intron 30.0%
! TATACTGGTGATTGTTATTC+TGG - chr5.3:46627021-46627040 MS.gene74006:CDS 30.0%
!! ACTCTGTTACATTTTGATGT+AGG - chr5.3:46628054-46628073 MS.gene74006:intron 30.0%
!! GTTACATTTTGATGTAGGAA+TGG - chr5.3:46628059-46628078 MS.gene74006:intron 30.0%
!! TATTTTGGAGACAAAATGCA+TGG - chr5.3:46628101-46628120 MS.gene74006:intron 30.0%
!!! AAATATACAGGGGAGTATTT+TGG - chr5.3:46628086-46628105 MS.gene74006:intron 30.0%
!!! ATGTGATTTTCTGAGATTAC+TGG + chr5.3:46627272-46627291 None:intergenic 30.0%
!!! GTTTTAAAACATTCATGGGA+CGG - chr5.3:46626652-46626671 MS.gene74006:intron 30.0%
!!! TTTTAAAACATTCATGGGAC+GGG - chr5.3:46626653-46626672 MS.gene74006:intron 30.0%
AAAAAGAAGAAAACCCGGTT+CGG - chr5.3:46626397-46626416 MS.gene74006:intron 35.0%
AAAGCTGTTCAGGATAGAAT+GGG - chr5.3:46629032-46629051 MS.gene74006:CDS 35.0%
ACGCAAACAGAATATCATTC+GGG + chr5.3:46628552-46628571 None:intergenic 35.0%
AGAACGAAAAGAACGTGAAA+GGG - chr5.3:46626758-46626777 MS.gene74006:intron 35.0%
ATGGTTATGGAATTGAAAGC+TGG - chr5.3:46626929-46626948 MS.gene74006:intron 35.0%
ATTTATAATTCCACGGGTAC+TGG + chr5.3:46628630-46628649 None:intergenic 35.0%
CATTCGGGTAATATTTCCAA+AGG + chr5.3:46628537-46628556 None:intergenic 35.0%
GAAGTTGAATTCTACAGCAA+TGG - chr5.3:46626799-46626818 MS.gene74006:intron 35.0%
GTAGATGGCATAAACCAATT+AGG + chr5.3:46628420-46628439 None:intergenic 35.0%
GTGAAAGGGTTGTTAAAAGA+GGG - chr5.3:46626772-46626791 MS.gene74006:intron 35.0%
TCTGATGGAAGTTGTTATGT+TGG - chr5.3:46627090-46627109 MS.gene74006:CDS 35.0%
TCTGTATTAGTGTTGTGTGT+CGG - chr5.3:46627301-46627320 MS.gene74006:intron 35.0%
TGGAAGATATGAAGGTGATT+GGG - chr5.3:46626891-46626910 MS.gene74006:intron 35.0%
TGGTTATGGAATTGAAAGCT+GGG - chr5.3:46626930-46626949 MS.gene74006:intron 35.0%
TTCCAAAGGAAGTGAAAAAC+CGG - chr5.3:46626699-46626718 MS.gene74006:intron 35.0%
TTCTACGAAGGAGAATTTCA+TGG - chr5.3:46626826-46626845 MS.gene74006:intron 35.0%
TTTGTGAGTGGAAGATATGA+AGG - chr5.3:46626883-46626902 MS.gene74006:intron 35.0%
! AATGGACAAAGTCATGGTTT+TGG - chr5.3:46627054-46627073 MS.gene74006:CDS 35.0%
! GTCTTGGTTGTTACCATTTT+AGG - chr5.3:46627139-46627158 MS.gene74006:CDS 35.0%
! TACTGGTGATTGTTATTCTG+GGG - chr5.3:46627023-46627042 MS.gene74006:CDS 35.0%
! TATTCTGGGGAATGGTTTAA+TGG - chr5.3:46627036-46627055 MS.gene74006:CDS 35.0%
! TGGTTTAATGGACAAAGTCA+TGG - chr5.3:46627048-46627067 MS.gene74006:CDS 35.0%
! TTCATGGTGGAAAGTGTAAT+GGG - chr5.3:46626842-46626861 MS.gene74006:intron 35.0%
! TTCTCATTCTCGTTCAAGTT+CGG - chr5.3:46626420-46626439 MS.gene74006:intron 35.0%
! TTGGTAGTCCTCAAATTCTA+TGG - chr5.3:46627714-46627733 MS.gene74006:intron 35.0%
! TTTCATGGTGGAAAGTGTAA+TGG - chr5.3:46626841-46626860 MS.gene74006:intron 35.0%
!! ATTTTTGATTGGTACTGTCG+TGG - chr5.3:46628719-46628738 MS.gene74006:CDS 35.0%
!! TGGGTTGATGGAAAATATGA+TGG - chr5.3:46626910-46626929 MS.gene74006:intron 35.0%
!! TTTGGTTCTAAACATGGTCT+TGG - chr5.3:46627123-46627142 MS.gene74006:CDS 35.0%
!!! AGGTCTGCTGTTTTTTAAGA+AGG + chr5.3:46628400-46628419 None:intergenic 35.0%
!!! TTTTTCCATATCGGTTCAGT+GGG - chr5.3:46626532-46626551 MS.gene74006:intron 35.0%
!!! TTTTTTCCATATCGGTTCAG+TGG - chr5.3:46626531-46626550 MS.gene74006:intron 35.0%
AACAGTGTCCATTCGCAAAA+TGG + chr5.3:46628139-46628158 None:intergenic 40.0%
ACACGAGTGAAGATTCAAAC+TGG + chr5.3:46628853-46628872 None:intergenic 40.0%
AGCAATGGTGATTTCTACGA+AGG - chr5.3:46626814-46626833 MS.gene74006:intron 40.0%
AGTACTCCGAAACTTGTAGA+TGG + chr5.3:46628435-46628454 None:intergenic 40.0%
CCAAAGGAGTTGCTACAATT+TGG + chr5.3:46628521-46628540 None:intergenic 40.0%
CCAAATTGTAGCAACTCCTT+TGG - chr5.3:46628518-46628537 MS.gene74006:CDS 40.0%
CGTGAAAGGGTTGTTAAAAG+AGG - chr5.3:46626771-46626790 MS.gene74006:intron 40.0%
GAAAGCTGTTCAGGATAGAA+TGG - chr5.3:46629031-46629050 MS.gene74006:CDS 40.0%
GAACCAAATCCTCTTTGCTT+CGG - chr5.3:46626251-46626270 MS.gene74006:CDS 40.0%
GAGAACGAAAAGAACGTGAA+AGG - chr5.3:46626757-46626776 MS.gene74006:intron 40.0%
GGAGACAAAATGCATGGATT+TGG - chr5.3:46628107-46628126 MS.gene74006:intron 40.0%
GGTGTTCAATCTTGTTCTGA+TGG - chr5.3:46627075-46627094 MS.gene74006:CDS 40.0%
GTGGAAGATATGAAGGTGAT+TGG - chr5.3:46626890-46626909 MS.gene74006:intron 40.0%
TACGAAGGAGAATTTCATGG+TGG - chr5.3:46626829-46626848 MS.gene74006:intron 40.0%
TCACCTATGAACCATTGAAC+CGG + chr5.3:46626721-46626740 None:intergenic 40.0%
TCTCCACTTGGTCATCATTT+CGG + chr5.3:46628959-46628978 None:intergenic 40.0%
TGGGCACAAACTATGAAGTA+CGG - chr5.3:46627204-46627223 MS.gene74006:CDS 40.0%
! AAACCGGTTCAATGGTTCAT+AGG - chr5.3:46626715-46626734 MS.gene74006:intron 40.0%
! AAGCAAAGAGGATTTGGTTC+GGG + chr5.3:46626251-46626270 None:intergenic 40.0%
! AGCAACACATTTTCGGAAGT+GGG + chr5.3:46626559-46626578 None:intergenic 40.0%
! CAAGGTTTGAGACATGGTTA+TGG - chr5.3:46626985-46627004 MS.gene74006:intron 40.0%
! GAAGCAAAGAGGATTTGGTT+CGG + chr5.3:46626252-46626271 None:intergenic 40.0%
! GAAGTGAAAAACCGGTTCAA+TGG - chr5.3:46626707-46626726 MS.gene74006:intron 40.0%
! GACGGGAATTTAAAACGACT+CGG - chr5.3:46626670-46626689 MS.gene74006:intron 40.0%
! GTGATTGTTATTCTGGGGAA+TGG - chr5.3:46627028-46627047 MS.gene74006:CDS 40.0%
! TCTCATTCTCGTTCAAGTTC+GGG - chr5.3:46626421-46626440 MS.gene74006:intron 40.0%
!! AACCGGTTTTTCACTTCCTT+TGG + chr5.3:46626704-46626723 None:intergenic 40.0%
!! CTAGACAGCGAAATGGATTT+TGG + chr5.3:46628686-46628705 None:intergenic 40.0%
!! TATGAAGGTGATTGGGTTGA+TGG - chr5.3:46626898-46626917 MS.gene74006:intron 40.0%
AACGAGAATGAGAACCGAAC+CGG + chr5.3:46626414-46626433 None:intergenic 45.0%
AAGCAGTAATGGCAGCAAAC+AGG - chr5.3:46628979-46628998 MS.gene74006:CDS 45.0%
ACACCGAAGCAAAGAGGATT+TGG + chr5.3:46626257-46626276 None:intergenic 45.0%
AGAAGAACACCGAAGCAAAG+AGG + chr5.3:46626263-46626282 None:intergenic 45.0%
AGACCGAAGATGTGGTGAAT+GGG - chr5.3:46628220-46628239 MS.gene74006:CDS 45.0%
AGCAGTAATGGCAGCAAACA+GGG - chr5.3:46628980-46628999 MS.gene74006:CDS 45.0%
ATGGAATTGAAAGCTGGGCT+AGG - chr5.3:46626935-46626954 MS.gene74006:intron 45.0%
ATTGGTACTGTCGTGGTCTT+TGG - chr5.3:46628726-46628745 MS.gene74006:CDS 45.0%
CAAATACCTGAGCTGATGCA+AGG + chr5.3:46628292-46628311 None:intergenic 45.0%
CAATGGTTCATAGGTGAACC+CGG - chr5.3:46626724-46626743 MS.gene74006:intron 45.0%
CCAAGTGGAGAAAGCAGTAA+TGG - chr5.3:46628968-46628987 MS.gene74006:intron 45.0%
CCATTACTGCTTTCTCCACT+TGG + chr5.3:46628971-46628990 None:intergenic 45.0%
CTCCACTTGGTCATCATTTC+GGG + chr5.3:46628958-46628977 None:intergenic 45.0%
GACACTGTTATGAAGGAGCA+TGG - chr5.3:46628150-46628169 MS.gene74006:CDS 45.0%
GATTATGCATGTCCTGGTGT+TGG + chr5.3:46627340-46627359 None:intergenic 45.0%
GCGAATGGACACTGTTATGA+AGG - chr5.3:46628143-46628162 MS.gene74006:CDS 45.0%
GCTAGGGGGAGTAGATATAA+GGG - chr5.3:46626952-46626971 MS.gene74006:intron 45.0%
GTTCGTCTCATACTGAGTCA+AGG + chr5.3:46627399-46627418 None:intergenic 45.0%
GTTGCTACTAGACAGCGAAA+TGG + chr5.3:46628693-46628712 None:intergenic 45.0%
TACTGTCGTGGTCTTTGGTT+CGG - chr5.3:46628731-46628750 MS.gene74006:intron 45.0%
TATGAAGCACGAACACTCCT+CGG + chr5.3:46627362-46627381 None:intergenic 45.0%
TCCACTTGGTCATCATTTCG+GGG + chr5.3:46628957-46628976 None:intergenic 45.0%
TGGAATTGAAAGCTGGGCTA+GGG - chr5.3:46626936-46626955 MS.gene74006:intron 45.0%
TGGTTAAATTGCAGGCTGCT+AGG - chr5.3:46628904-46628923 MS.gene74006:intron 45.0%
TGTCCCATTCACCACATCTT+CGG + chr5.3:46628226-46628245 None:intergenic 45.0%
TTCGTTCTCCTCTGTTGAAC+CGG + chr5.3:46626746-46626765 None:intergenic 45.0%
TTTAATGGTTCCAGTACCCG+TGG - chr5.3:46628617-46628636 MS.gene74006:CDS 45.0%
! CAGCAACACATTTTCGGAAG+TGG + chr5.3:46626560-46626579 None:intergenic 45.0%
! GGAGTCTACCATTTTGCGAA+TGG - chr5.3:46628128-46628147 MS.gene74006:intron 45.0%
! TATGAAGGAGCATGGCATGA+AGG - chr5.3:46628158-46628177 MS.gene74006:CDS 45.0%
! TCTCGTTCAAGTTCGGGTTA+TGG - chr5.3:46626427-46626446 MS.gene74006:intron 45.0%
! TCTGAGATTACTGGCAGTGT+CGG + chr5.3:46627263-46627282 None:intergenic 45.0%
! TGGTTCGGGTGAGTTTAGTT+TGG + chr5.3:46626237-46626256 None:intergenic 45.0%
!! AGGAGTGTTCGTGCTTCATA+GGG - chr5.3:46627362-46627381 MS.gene74006:intron 45.0%
!!! CGACTCGGTTTTTCTTCCAA+AGG - chr5.3:46626685-46626704 MS.gene74006:intron 45.0%
!!! TAATAAAATATTTTATGTAT+TGG - chr5.3:46627695-46627714 MS.gene74006:intron 5.0%
ACCAGGACATGCATAATCCG+AGG - chr5.3:46627342-46627361 MS.gene74006:intron 50.0%
ACGAGAATGAGAACCGAACC+GGG + chr5.3:46626413-46626432 None:intergenic 50.0%
AGATGTGGTGAATGGGACAG+TGG - chr5.3:46628227-46628246 MS.gene74006:CDS 50.0%
CACAACACATGTCCAACACC+AGG - chr5.3:46627325-46627344 MS.gene74006:intron 50.0%
CGAAATGGAGACCGAAGATG+TGG - chr5.3:46628212-46628231 MS.gene74006:CDS 50.0%
CTTCTTCGTTGACGGAACCT+AGG + chr5.3:46626324-46626343 None:intergenic 50.0%
CTTGGTCATCATTTCGGGGA+TGG + chr5.3:46628953-46628972 None:intergenic 50.0%
GAAGGTCGTCGACAAGGTTA+TGG - chr5.3:46628176-46628195 MS.gene74006:CDS 50.0%
GAGACCGAAGATGTGGTGAA+TGG - chr5.3:46628219-46628238 MS.gene74006:CDS 50.0%
GCTGTTCAGGATAGAATGGG+TGG - chr5.3:46629035-46629054 MS.gene74006:CDS 50.0%
GGCTAGGGGGAGTAGATATA+AGG - chr5.3:46626951-46626970 MS.gene74006:intron 50.0%
TCCCCGAAATGATGACCAAG+TGG - chr5.3:46628953-46628972 MS.gene74006:intron 50.0%
TCCTCGGATTATGCATGTCC+TGG + chr5.3:46627346-46627365 None:intergenic 50.0%
TCGTTCTCCTCTGTTGAACC+GGG + chr5.3:46626745-46626764 None:intergenic 50.0%
TGCAGCAGTGAAAGCTGTTC+AGG - chr5.3:46629022-46629041 MS.gene74006:CDS 50.0%
! GAATTGAAAGCTGGGCTAGG+GGG - chr5.3:46626938-46626957 MS.gene74006:intron 50.0%
! GGAATTGAAAGCTGGGCTAG+GGG - chr5.3:46626937-46626956 MS.gene74006:intron 50.0%
! GGTGGAAAGTGTAATGGGAG+TGG - chr5.3:46626847-46626866 MS.gene74006:intron 50.0%
! GTTCGGGTTATGGTTCAACC+CGG - chr5.3:46626437-46626456 MS.gene74006:intron 50.0%
!! ACTACTGCTGCACCGATTCT+AGG - chr5.3:46626469-46626488 MS.gene74006:intron 50.0%
!! GAGGAGTGTTCGTGCTTCAT+AGG - chr5.3:46627361-46627380 MS.gene74006:intron 50.0%
AGGTGAACCCGGTTCAACAG+AGG - chr5.3:46626735-46626754 MS.gene74006:intron 55.0%
AGTACGGATACAGACACGCC+TGG - chr5.3:46627220-46627239 MS.gene74006:intron 55.0%
TCGTTGACGGAACCTAGGCT+CGG + chr5.3:46626319-46626338 None:intergenic 55.0%
TGAGGCTTGGAGTGGAGGAT+CGG + chr5.3:46626294-46626313 None:intergenic 55.0%
TGCAACTCTAGCAGCAGTGG+CGG + chr5.3:46629006-46629025 None:intergenic 55.0%
TGCTGCAACTCTAGCAGCAG+TGG + chr5.3:46629009-46629028 None:intergenic 55.0%
TGGCATGAAGGTCGTCGACA+AGG - chr5.3:46628170-46628189 MS.gene74006:CDS 55.0%
TGGGTCCCACTGAACCGATA+TGG + chr5.3:46626540-46626559 None:intergenic 55.0%
!! CGTGGTCTTTGGTTCGGTAC+TGG - chr5.3:46628737-46628756 MS.gene74006:intron 55.0%
AGGACAGCCTGCGTTTGTGG+CGG + chr5.3:46628272-46628291 None:intergenic 60.0%
CCAAGCCTCATTCCGAGCCT+AGG - chr5.3:46626304-46626323 MS.gene74006:CDS 60.0%
CCTAGGCTCGGAATGAGGCT+TGG + chr5.3:46626307-46626326 None:intergenic 60.0%
CGGAACCTAGGCTCGGAATG+AGG + chr5.3:46626312-46626331 None:intergenic 60.0%
CGGAATGAGGCTTGGAGTGG+AGG + chr5.3:46626299-46626318 None:intergenic 60.0%
CTCTCTACCGCCACAAACGC+AGG - chr5.3:46628262-46628281 MS.gene74006:CDS 60.0%
GAGGCTTGGAGTGGAGGATC+GGG + chr5.3:46626293-46626312 None:intergenic 60.0%
GCAAGGACAGCCTGCGTTTG+TGG + chr5.3:46628275-46628294 None:intergenic 60.0%
GCGTGTGAGTGTCAGTGTCC+AGG + chr5.3:46627241-46627260 None:intergenic 60.0%
GCTCGGAATGAGGCTTGGAG+TGG + chr5.3:46626302-46626321 None:intergenic 60.0%
GGCTGTCCTTGCATCAGCTC+AGG - chr5.3:46628283-46628302 MS.gene74006:CDS 60.0%
!! GTGGCGGTAGAGAGTGCTTG+AGG + chr5.3:46628256-46628275 None:intergenic 60.0%
GCTTGGAGTGGAGGATCGGG+TGG + chr5.3:46626290-46626309 None:intergenic 65.0%
GGAGTGGAGGATCGGGTGGT+TGG + chr5.3:46626286-46626305 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 46626217 46629082 46626217 ID=MS.gene74006
chr5.3 mRNA 46626217 46629082 46626217 ID=MS.gene74006.t1;Parent=MS.gene74006
chr5.3 exon 46628974 46629082 46628974 ID=MS.gene74006.t1.exon1;Parent=MS.gene74006.t1
chr5.3 CDS 46628974 46629082 46628974 ID=cds.MS.gene74006.t1;Parent=MS.gene74006.t1
chr5.3 exon 46628139 46628748 46628139 ID=MS.gene74006.t1.exon2;Parent=MS.gene74006.t1
chr5.3 CDS 46628139 46628748 46628139 ID=cds.MS.gene74006.t1;Parent=MS.gene74006.t1
chr5.3 exon 46626995 46627223 46626995 ID=MS.gene74006.t1.exon3;Parent=MS.gene74006.t1
chr5.3 CDS 46626995 46627223 46626995 ID=cds.MS.gene74006.t1;Parent=MS.gene74006.t1
chr5.3 exon 46626217 46626381 46626217 ID=MS.gene74006.t1.exon4;Parent=MS.gene74006.t1
chr5.3 CDS 46626217 46626381 46626217 ID=cds.MS.gene74006.t1;Parent=MS.gene74006.t1
Gene Sequence

>MS.gene74006

ATGGAAACAAACAATAGCCAAACTAAACTCACCCGAACCAAATCCTCTTTGCTTCGGTGTTCTTCTCCAACCACCCGATCCTCCACTCCAAGCCTCATTCCGAGCCTAGGTTCCGTCAACGAAGAAGATTTTTACGATGAAGAAGAACATGATAAAAACAAGTACAAAAACAAAAATAAGAAAAAGAAGAAAACCCGGTTCGGTTCTCATTCTCGTTCAAGTTCGGGTTATGGTTCAACCCGGTTTATTATTACTACTGCTGCACCGATTCTAGGTTTTTTCAGTCTATGTTCTATCTTCTTCTATGTATTCTATTTTTTCCATATCGGTTCAGTGGGACCCACTTCCGAAAATGTGTTGCTGATTCTAATATTCATCGCCGTAGCGCTTTATTTCATAAACAGAAACAAACGCGTGATTCATAGAACCGTTTCTGTTTTAAAACATTCATGGGACGGGAATTTAAAACGACTCGGTTTTTCTTCCAAAGGAAGTGAAAAACCGGTTCAATGGTTCATAGGTGAACCCGGTTCAACAGAGGAGAACGAAAAGAACGTGAAAGGGTTGTTAAAAGAGGGTGTTGAAGTTGAATTCTACAGCAATGGTGATTTCTACGAAGGAGAATTTCATGGTGGAAAGTGTAATGGGAGTGGAGTTTATCATTATTTTGTGAGTGGAAGATATGAAGGTGATTGGGTTGATGGAAAATATGATGGTTATGGAATTGAAAGCTGGGCTAGGGGGAGTAGATATAAGGGTTGTTATAAACAAGGTTTGAGACATGGTTATGGTGTTTATAGATTTTATACTGGTGATTGTTATTCTGGGGAATGGTTTAATGGACAAAGTCATGGTTTTGGTGTTCAATCTTGTTCTGATGGAAGTTGTTATGTTGGTGAATTTAAGTTTGGTTCTAAACATGGTCTTGGTTGTTACCATTTTAGGTGAGTTTTTTTTTCTTTCTTGTTCTCAAGTTTTTATTGAAATTGGGCACAAACTATGAAGTACGGATACAGACACGCCTGGACACTGACACTCACACGCCGACACTGCCAGTAATCTCAGAAAATCACATAATTCAGTGTCTGTATTAGTGTTGTGTGTCGGACACAACACATGTCCAACACCAGGACATGCATAATCCGAGGAGTGTTCGTGCTTCATAGGGCACAAATTAGTCCTTGACTCAGTATGAGACGAACTAATTTGTTCCCGATATGAAAATGTTATGAACTAAAGCATGAAAATTGTGAAAGACCAAAATGGTTCTTCACTCATATTTTTACTTTTAATTTTGCAATTGCAATTTTGTTTGTTAATTTTTTTTTTCACTATTTGACGATTTATGTTCTTTTGGGTATGCAATTTTTGAATCTTTAATGATAGATATAATATACTCCTCAAATAAAATAGTCCTCAAATAAAGTAGACTCTATTGTTGATTTTTGGAATTTTTCTTTGTTCTATACGTTGTTTATTAATAAAATATTTTATGTATTGGTAGTCCTCAAATTCTATGGTGTAATTTAATTTGCTGAAATTGCTGAATTTTGTTGCTAGTGTTGAATATTTATTTCATGTTTCTTCATGTTTAATTCAACTATACATTGTTTGACAAGTTAAAAGGGAAAGTATTATATTTGTCAAGACTTTTTCTTGAAGAAAATATTTGTCAAGACCTTGTTAATGTCATTTGACAATATGCAAAAGCAATAGTAGTAGGTTGTTGTGTTGTAAAGTTTTGACTAAACCATTATTATTATATAGGAAGCTATGATAATAGACCTTAGACTATAGTTGGTTATGTATTTTAAGTTTTATTTAACTAAATAAACTTACTCTGTTACATTTTGATGTAGGAATGGAGATAAATATACAGGGGAGTATTTTGGAGACAAAATGCATGGATTTGGAGTCTACCATTTTGCGAATGGACACTGTTATGAAGGAGCATGGCATGAAGGTCGTCGACAAGGTTATGGTGTTTATACATTTCGAAATGGAGACCGAAGATGTGGTGAATGGGACAGTGGTGACCTCAAGCACTCTCTACCGCCACAAACGCAGGCTGTCCTTGCATCAGCTCAGGTATTTGTCATTAAGACTTACTCTTTTTGCCTTTTCCTAAAAATAAACTCATTCAAAAAATGATTATTTCACAAATTAATATCTAAATCGGTCCTTCTTAAAAAACAGCAGACCTAATTGGTTTATGCCATCTACAAGTTTCGGAGTACTTAAGTTTCTGTACATCTTATTTAAAAGTTGACTAATTAGACTATTATTTGAGATTTTTGACGACCAAATTGTAGCAACTCCTTTGGAAATATTACCCGAATGATATTCTGTTTGCGTATTTCAATTACTTACTATAGTACCTAATAAATTATTGAGCGTTCTTTTAATGGTTCCAGTACCCGTGGAATTATAAATAGTAGTACCTTTTATGGAAAATGTTAGTAAGTTCCAAAATCCATTTCGCTGTCTAGTAGCAACAATCATATTTTTGATTGGTACTGTCGTGGTCTTTGGTTCGGTACTGGAGCAACATTACCTGTTGAAAATATCCCTATTTTTAGATCCTTACTTTTCATGTTACAGTGACCAAATAGATCTCTGTATTTTTTAGATGACCAGTTTGAATCTTCACTCGTGTTTTGCTCTAATTCATTTACGTTAATTTTTTTTGGTTAAATTGCAGGCTGCTAGGAGAACAGCAGAAAATGCGATAAACCATCCCCGAAATGATGACCAAGTGGAGAAAGCAGTAATGGCAGCAAACAGGGCCGCCACTGCTGCTAGAGTTGCAGCAGTGAAAGCTGTTCAGGATAGAATGGGTGGAAAATTTTGCGATGATCAAGTTTAA
Protein sequence

>MS.gene74006.t1

METNNSQTKLTRTKSSLLRCSSPTTRSSTPSLIPSLVGPTSENVLLILIFIAVALYFINRNKRVIHRTVSVLKHSWDGNLKRLGFSSKGSEKPVQWFIGEPGSTEENEKNVKGLLKEGVEVEFYSNGDFYEGEFHGGKCNGSGVYHYFVSGRYEGDWVDGKYDGYGIESWARGSRYKGCYKQGLRHGYGVYRFYTGDCYSGEWFNGQSHGFGVQSCSDGSCYVGEFKFGSKHGLGCYHFRNGDKYTGEYFGDKMHGFGVYHFANGHCYEGAWHEGRRQGYGVYTFRNGDRRCGEWDSGDLKHSLPPQTQAVLASAQAARRTAENAINHPRNDDQVEKAVMAANRAATAARVAAVKAVQDRMGGKFCDDQV