AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74025


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74025.t1 MTR_7g106870 85.124 242 6 2 1 212 216 457 6.39e-147 417
MS.gene74025.t1 MTR_5g008010 70.124 241 43 1 1 212 214 454 1.97e-123 357
MS.gene74025.t1 MTR_3g051690 68.182 242 47 2 1 212 218 459 1.17e-118 345
MS.gene74025.t1 MTR_7g034625 73.128 227 30 2 1 196 103 329 1.30e-115 333
MS.gene74025.t1 MTR_7g057540 68.313 243 41 4 1 212 189 426 7.91e-113 329
MS.gene74025.t1 MTR_7g055590 68.182 242 41 4 1 211 189 425 6.63e-111 327
MS.gene74025.t1 MTR_7g053100 65.432 243 50 3 1 212 197 436 4.06e-104 307
MS.gene74025.t1 MTR_7g451830 63.900 241 49 2 1 212 200 431 6.21e-104 306
MS.gene74025.t1 MTR_7g047640 63.900 241 49 2 1 212 200 431 6.21e-104 306
MS.gene74025.t1 MTR_2g100460 61.086 221 65 3 7 210 166 382 5.34e-87 262
MS.gene74025.t1 MTR_2g100540 54.506 233 38 7 7 208 196 391 7.85e-70 218
MS.gene74025.t1 MTR_3g067940 48.113 212 86 2 1 189 248 458 2.13e-65 209
MS.gene74025.t1 MTR_2g060710 47.685 216 81 4 7 191 216 430 3.00e-62 200
MS.gene74025.t1 MTR_4g102510 46.479 213 90 2 1 190 244 455 4.45e-62 200
MS.gene74025.t1 MTR_4g415070 61.818 55 21 0 137 191 57 111 2.49e-18 82.0
MS.gene74025.t1 MTR_2g012630 27.429 175 101 5 6 178 97 247 1.76e-11 63.2
MS.gene74025.t1 MTR_2g012630 27.429 175 101 5 6 178 97 247 2.31e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74025.t1 AT4G33270 71.784 241 39 1 1 212 217 457 3.48e-123 356
MS.gene74025.t1 AT4G33260 71.784 241 39 1 1 212 207 447 6.59e-118 343
MS.gene74025.t1 AT5G27570 66.667 243 50 3 1 212 208 450 8.79e-108 317
MS.gene74025.t1 AT4G33260 69.643 224 39 1 1 195 207 430 3.00e-107 315
MS.gene74025.t1 AT5G27945 65.289 242 54 2 1 212 187 428 7.28e-107 314
MS.gene74025.t1 AT5G27080 64.198 243 56 3 1 212 200 442 2.26e-103 305
MS.gene74025.t1 AT5G26900 64.050 242 57 2 1 212 203 444 3.36e-103 305
MS.gene74025.t1 AT5G13840 48.585 212 85 2 1 189 250 460 2.42e-66 212
MS.gene74025.t1 AT5G13840 48.585 212 85 2 1 189 250 460 2.42e-66 212
MS.gene74025.t1 AT4G22910 47.170 212 88 2 2 190 253 463 1.37e-64 207
MS.gene74025.t1 AT4G11920 46.009 213 91 2 1 190 244 455 1.71e-62 201

Find 64 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGATGAAACTCTTAGATTT+TGG 0.143277 7.3:-13289351 MS.gene74025:CDS
GTGGCTTTGCATGCTTCTGC+TGG 0.230906 7.3:+13289313 None:intergenic
CCTTTGGAAGTATCCTTCTA+TGG 0.254046 7.3:-13289461 MS.gene74025:CDS
CAGGGTAATCTACTGGCTTC+TGG 0.309255 7.3:-13289640 MS.gene74025:CDS
GTATCCTTCTATGGTGAAAA+TGG 0.311467 7.3:-13289452 MS.gene74025:CDS
ACGATAATGTTGTTCACATA+TGG 0.371186 7.3:-13289757 MS.gene74025:CDS
AATCTACTGGCTTCTGGTGG+AGG 0.376592 7.3:-13289634 MS.gene74025:CDS
GTCATCATCTTCAACGAATA+CGG 0.384451 7.3:+13289254 None:intergenic
TTAGTAGCTCACGCTCATTC+TGG 0.385492 7.3:+13289515 None:intergenic
CACTTCGAGGGTGATGTATA+TGG 0.388915 7.3:-13289413 MS.gene74025:CDS
TGGCTTTGCATGCTTCTGCT+GGG 0.422084 7.3:+13289314 None:intergenic
CCGAGAACCAGCTTACCCTT+TGG 0.427049 7.3:-13289477 MS.gene74025:CDS
CTCACATCGTTGAAACATAC+AGG 0.428220 7.3:-13289847 MS.gene74025:CDS
CTACTGGCTTCTGGTGGAGG+TGG 0.431389 7.3:-13289631 MS.gene74025:CDS
AACAATCATATTCTGACAAC+AGG 0.439142 7.3:-13289914 MS.gene74025:CDS
CTGGCTTCTGGTGGAGGTGG+AGG 0.440261 7.3:-13289628 MS.gene74025:CDS
CAAGAGTGGGTTCACTGTCT+TGG 0.449748 7.3:-13289937 MS.gene74025:CDS
CGCAAGTATGTGCTCTGCTA+TGG 0.473307 7.3:-13289540 MS.gene74025:CDS
GATGCCACTGTACACCCATC+TGG 0.480407 7.3:+13289382 None:intergenic
ATCTCAACGGTCACACTTCG+AGG 0.480528 7.3:-13289426 MS.gene74025:CDS
CCAAAGGGTAAGCTGGTTCT+CGG 0.481991 7.3:+13289477 None:intergenic
ACTGCAGCTAAGGGCAATGA+AGG 0.483352 7.3:-13289972 None:intergenic
CGATAATGTTGTTCACATAT+GGG 0.487900 7.3:-13289756 MS.gene74025:CDS
TGTCCCTCAGGGTAATCTAC+TGG 0.491265 7.3:-13289647 MS.gene74025:intron
AAGGGCTCATTATATGACTT+TGG 0.495746 7.3:+13289289 None:intergenic
GGACACAACCAGGAGGTTTG+TGG 0.497262 7.3:-13289824 MS.gene74025:CDS
GATACTTCCAAAGGGTAAGC+TGG 0.503616 7.3:+13289470 None:intergenic
GACACAACCAGGAGGTTTGT+GGG 0.510860 7.3:-13289823 MS.gene74025:CDS
AACATACAGGGGACACAACC+AGG 0.511067 7.3:-13289834 MS.gene74025:CDS
CTCATTATATGACTTTGGTG+TGG 0.524026 7.3:+13289294 None:intergenic
TATTCTGACAACAGGAGGAA+TGG 0.524698 7.3:-13289906 MS.gene74025:CDS
GGGCTCAAGTGGTCACCCTC+AGG 0.528586 7.3:-13289803 MS.gene74025:CDS
AGGAGGTTTGTGGGCTCAAG+TGG 0.528883 7.3:-13289814 MS.gene74025:CDS
ACTTGAGCCCACAAACCTCC+TGG 0.538177 7.3:+13289816 None:intergenic
TCATCATCTTCAACGAATAC+GGG 0.547121 7.3:+13289255 None:intergenic
GGGTGGACACAGAGCAAGAG+TGG 0.549117 7.3:-13289951 MS.gene74025:CDS
GGTAATCTACTGGCTTCTGG+TGG 0.565300 7.3:-13289637 MS.gene74025:CDS
TATATGGCGCAAAGTCCAGA+TGG 0.565373 7.3:-13289397 MS.gene74025:CDS
CGTGAGCTACTAAGCTCACA+TGG 0.568122 7.3:-13289505 MS.gene74025:CDS
CTGACAACAGGAGGAATGGA+TGG 0.572412 7.3:-13289902 MS.gene74025:CDS
CATAGAAGGATACTTCCAAA+GGG 0.584766 7.3:+13289462 None:intergenic
TCACATATGGGATAGATCTG+TGG 0.596581 7.3:-13289744 MS.gene74025:intron
AGATTGAATTCAGTTGACAC+TGG 0.597762 7.3:-13289565 MS.gene74025:CDS
GGACAACAATTGTCAAGTGG+TGG 0.599505 7.3:-13289782 MS.gene74025:CDS
ATACGGGTGAAATTAGCAAA+GGG 0.600657 7.3:+13289271 None:intergenic
TCTCAACGGTCACACTTCGA+GGG 0.613321 7.3:-13289425 MS.gene74025:CDS
GTGAAAATGGCAGATCTCAA+CGG 0.613838 7.3:-13289439 MS.gene74025:CDS
AATACGGGTGAAATTAGCAA+AGG 0.619999 7.3:+13289270 None:intergenic
CAGCTAAGGGCAATGAAGGG+TGG 0.622963 7.3:-13289968 MS.gene74025:CDS
CCATAGAAGGATACTTCCAA+AGG 0.623454 7.3:+13289461 None:intergenic
AAGCTGTGGAACACACACAC+TGG 0.630912 7.3:-13289592 MS.gene74025:CDS
GTGATCATTGCATTAAGCTG+TGG 0.635919 7.3:-13289606 MS.gene74025:CDS
AATCATATTCTGACAACAGG+AGG 0.641326 7.3:-13289911 MS.gene74025:CDS
ACTTGACAATTGTTGTCCTG+AGG 0.654097 7.3:+13289787 None:intergenic
TCACATCGTTGAAACATACA+GGG 0.656486 7.3:-13289846 MS.gene74025:CDS
CTTGACAATTGTTGTCCTGA+GGG 0.657303 7.3:+13289788 None:intergenic
TCAGGACAACAATTGTCAAG+TGG 0.664718 7.3:-13289785 MS.gene74025:CDS
TGGGTGTACAGTGGCATCTG+CGG 0.666837 7.3:-13289377 MS.gene74025:CDS
CTGCAGCTAAGGGCAATGAA+GGG 0.668716 7.3:-13289971 None:intergenic
GGTGGACACAGAGCAAGAGT+GGG 0.681424 7.3:-13289950 MS.gene74025:CDS
AAGTCCAGATGGGTGTACAG+TGG 0.686450 7.3:-13289386 MS.gene74025:CDS
ATATGGCGCAAAGTCCAGAT+GGG 0.692355 7.3:-13289396 MS.gene74025:CDS
CACATCGTTGAAACATACAG+GGG 0.758385 7.3:-13289845 MS.gene74025:CDS
ATACAGGGGACACAACCAGG+AGG 0.774768 7.3:-13289831 MS.gene74025:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTAGATTTTGGAATGTCTTT+GGG - chr7.3:13289890-13289909 MS.gene74025:CDS 25.0%
AACAATCATATTCTGACAAC+AGG - chr7.3:13289315-13289334 MS.gene74025:CDS 30.0%
ACGATAATGTTGTTCACATA+TGG - chr7.3:13289472-13289491 MS.gene74025:CDS 30.0%
CGATAATGTTGTTCACATAT+GGG - chr7.3:13289473-13289492 MS.gene74025:CDS 30.0%
! CAGATGAAACTCTTAGATTT+TGG - chr7.3:13289878-13289897 MS.gene74025:CDS 30.0%
!!! CTTAGATTTTGGAATGTCTT+TGG - chr7.3:13289889-13289908 MS.gene74025:CDS 30.0%
AAGGGCTCATTATATGACTT+TGG + chr7.3:13289943-13289962 None:intergenic 35.0%
AATACGGGTGAAATTAGCAA+AGG + chr7.3:13289962-13289981 None:intergenic 35.0%
AATCATATTCTGACAACAGG+AGG - chr7.3:13289318-13289337 MS.gene74025:CDS 35.0%
AGATTGAATTCAGTTGACAC+TGG - chr7.3:13289664-13289683 MS.gene74025:intron 35.0%
ATACGGGTGAAATTAGCAAA+GGG + chr7.3:13289961-13289980 None:intergenic 35.0%
CATAGAAGGATACTTCCAAA+GGG + chr7.3:13289770-13289789 None:intergenic 35.0%
CTCATTATATGACTTTGGTG+TGG + chr7.3:13289938-13289957 None:intergenic 35.0%
GTATCCTTCTATGGTGAAAA+TGG - chr7.3:13289777-13289796 MS.gene74025:CDS 35.0%
TCACATCGTTGAAACATACA+GGG - chr7.3:13289383-13289402 MS.gene74025:CDS 35.0%
ACTTGACAATTGTTGTCCTG+AGG + chr7.3:13289445-13289464 None:intergenic 40.0%
CACATCGTTGAAACATACAG+GGG - chr7.3:13289384-13289403 MS.gene74025:CDS 40.0%
CCATAGAAGGATACTTCCAA+AGG + chr7.3:13289771-13289790 None:intergenic 40.0%
CTCACATCGTTGAAACATAC+AGG - chr7.3:13289382-13289401 MS.gene74025:CDS 40.0%
CTTGACAATTGTTGTCCTGA+GGG + chr7.3:13289444-13289463 None:intergenic 40.0%
GTGAAAATGGCAGATCTCAA+CGG - chr7.3:13289790-13289809 MS.gene74025:CDS 40.0%
GTGATCATTGCATTAAGCTG+TGG - chr7.3:13289623-13289642 MS.gene74025:CDS 40.0%
TATTCTGACAACAGGAGGAA+TGG - chr7.3:13289323-13289342 MS.gene74025:CDS 40.0%
TCACATATGGGATAGATCTG+TGG - chr7.3:13289485-13289504 MS.gene74025:CDS 40.0%
TCAGGACAACAATTGTCAAG+TGG - chr7.3:13289444-13289463 MS.gene74025:CDS 40.0%
!! CCTTTGGAAGTATCCTTCTA+TGG - chr7.3:13289768-13289787 MS.gene74025:CDS 40.0%
!!! TCTGCCATTTTCACCATAGA+AGG + chr7.3:13289784-13289803 None:intergenic 40.0%
AAGCCAGTAGATTACCCTGA+GGG + chr7.3:13289588-13289607 None:intergenic 45.0%
ATATGGCGCAAAGTCCAGAT+GGG - chr7.3:13289833-13289852 MS.gene74025:CDS 45.0%
CACTTCGAGGGTGATGTATA+TGG - chr7.3:13289816-13289835 MS.gene74025:CDS 45.0%
CCTCAAACCTGTGAAGACAT+CGG + chr7.3:13289530-13289549 None:intergenic 45.0%
CTCAAACCTGTGAAGACATC+GGG + chr7.3:13289529-13289548 None:intergenic 45.0%
GATACTTCCAAAGGGTAAGC+TGG + chr7.3:13289762-13289781 None:intergenic 45.0%
GGACAACAATTGTCAAGTGG+TGG - chr7.3:13289447-13289466 MS.gene74025:CDS 45.0%
TATATGGCGCAAAGTCCAGA+TGG - chr7.3:13289832-13289851 MS.gene74025:CDS 45.0%
TTAGTAGCTCACGCTCATTC+TGG + chr7.3:13289717-13289736 None:intergenic 45.0%
AACATACAGGGGACACAACC+AGG - chr7.3:13289395-13289414 MS.gene74025:CDS 50.0%
AACCTGTGAAGACATCGGGT+AGG + chr7.3:13289525-13289544 None:intergenic 50.0%
AAGCTGTGGAACACACACAC+TGG - chr7.3:13289637-13289656 MS.gene74025:CDS 50.0%
AAGTCCAGATGGGTGTACAG+TGG - chr7.3:13289843-13289862 MS.gene74025:CDS 50.0%
ATCTCAACGGTCACACTTCG+AGG - chr7.3:13289803-13289822 MS.gene74025:CDS 50.0%
CCGATGTCTTCACAGGTTTG+AGG - chr7.3:13289527-13289546 MS.gene74025:CDS 50.0%
CGCAAGTATGTGCTCTGCTA+TGG - chr7.3:13289689-13289708 MS.gene74025:intron 50.0%
CGTGAGCTACTAAGCTCACA+TGG - chr7.3:13289724-13289743 MS.gene74025:intron 50.0%
CTGACAACAGGAGGAATGGA+TGG - chr7.3:13289327-13289346 MS.gene74025:CDS 50.0%
GAAGCCAGTAGATTACCCTG+AGG + chr7.3:13289589-13289608 None:intergenic 50.0%
GACACAACCAGGAGGTTTGT+GGG - chr7.3:13289406-13289425 MS.gene74025:CDS 50.0%
GGAACACATAGCTGCTGTGA+AGG - chr7.3:13289548-13289567 MS.gene74025:CDS 50.0%
TCTCAACGGTCACACTTCGA+GGG - chr7.3:13289804-13289823 MS.gene74025:CDS 50.0%
TGTCCCTCAGGGTAATCTAC+TGG - chr7.3:13289582-13289601 MS.gene74025:CDS 50.0%
! CAAGAGTGGGTTCACTGTCT+TGG - chr7.3:13289292-13289311 MS.gene74025:CDS 50.0%
! CCAAAGGGTAAGCTGGTTCT+CGG + chr7.3:13289755-13289774 None:intergenic 50.0%
! TGGCTTTGCATGCTTCTGCT+GGG + chr7.3:13289918-13289937 None:intergenic 50.0%
!! AATCTACTGGCTTCTGGTGG+AGG - chr7.3:13289595-13289614 MS.gene74025:CDS 50.0%
!! CAGGGTAATCTACTGGCTTC+TGG - chr7.3:13289589-13289608 MS.gene74025:CDS 50.0%
!! GGTAATCTACTGGCTTCTGG+TGG - chr7.3:13289592-13289611 MS.gene74025:CDS 50.0%
ACTTGAGCCCACAAACCTCC+TGG + chr7.3:13289416-13289435 None:intergenic 55.0%
AGGAGGTTTGTGGGCTCAAG+TGG - chr7.3:13289415-13289434 MS.gene74025:CDS 55.0%
ATACAGGGGACACAACCAGG+AGG - chr7.3:13289398-13289417 MS.gene74025:CDS 55.0%
CACCTACCCGATGTCTTCAC+AGG - chr7.3:13289520-13289539 MS.gene74025:CDS 55.0%
CCGAGAACCAGCTTACCCTT+TGG - chr7.3:13289752-13289771 MS.gene74025:CDS 55.0%
GATGCCACTGTACACCCATC+TGG + chr7.3:13289850-13289869 None:intergenic 55.0%
GGACACAACCAGGAGGTTTG+TGG - chr7.3:13289405-13289424 MS.gene74025:CDS 55.0%
GGTGGACACAGAGCAAGAGT+GGG - chr7.3:13289279-13289298 MS.gene74025:CDS 55.0%
TGGGTGTACAGTGGCATCTG+CGG - chr7.3:13289852-13289871 MS.gene74025:CDS 55.0%
! CATAGCTGCTGTGAAGGCAC+TGG - chr7.3:13289554-13289573 MS.gene74025:CDS 55.0%
! GTGGCTTTGCATGCTTCTGC+TGG + chr7.3:13289919-13289938 None:intergenic 55.0%
GGGTGGACACAGAGCAAGAG+TGG - chr7.3:13289278-13289297 MS.gene74025:CDS 60.0%
!! CACTGGCTTGGTGTCCCTCA+GGG - chr7.3:13289571-13289590 MS.gene74025:CDS 60.0%
!! CTACTGGCTTCTGGTGGAGG+TGG - chr7.3:13289598-13289617 MS.gene74025:CDS 60.0%
!! CTGCTGTGAAGGCACTGGCT+TGG - chr7.3:13289559-13289578 MS.gene74025:CDS 60.0%
CTGGCTTCTGGTGGAGGTGG+AGG - chr7.3:13289601-13289620 MS.gene74025:CDS 65.0%
GGGCTCAAGTGGTCACCCTC+AGG - chr7.3:13289426-13289445 MS.gene74025:CDS 65.0%
!! GCACTGGCTTGGTGTCCCTC+AGG - chr7.3:13289570-13289589 MS.gene74025:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 13289264 13289987 13289264 ID=MS.gene74025
chr7.3 mRNA 13289264 13289987 13289264 ID=MS.gene74025.t1;Parent=MS.gene74025
chr7.3 exon 13289745 13289987 13289745 ID=MS.gene74025.t1.exon1;Parent=MS.gene74025.t1
chr7.3 CDS 13289745 13289987 13289745 ID=cds.MS.gene74025.t1;Parent=MS.gene74025.t1
chr7.3 exon 13289264 13289659 13289264 ID=MS.gene74025.t1.exon2;Parent=MS.gene74025.t1
chr7.3 CDS 13289264 13289659 13289264 ID=cds.MS.gene74025.t1;Parent=MS.gene74025.t1
Gene Sequence

>MS.gene74025

CTAAGGGCAATGAAGGGTGGACACAGAGCAAGAGTGGGTTCACTGTCTTGGAACAATCATATTCTGACAACAGGAGGAATGGATGGTAAAGTTGTAAACAATGATGTTAGAGTGAGATCTCACATCGTTGAAACATACAGGGGACACAACCAGGAGGTTTGTGGGCTCAAGTGGTCACCCTCAGGACAACAATTGTCAAGTGGTGGAAACGATAATGTTGTTCACATATGGGATAGATCTGTGGTTTCTTCAAATTCACCTACCCGATGTCTTCACAGGTTTGAGGAACACATAGCTGCTGTGAAGGCACTGGCTTGGTGTCCCTCAGGGTAATCTACTGGCTTCTGGTGGAGGTGGAGGTGATCATTGCATTAAGCTGTGGAACACACACACTGGTGCGAGATTGAATTCAGTTGACACTGGATCGCAAGTATGTGCTCTGCTATGGAACCAGAATGAGCGTGAGCTACTAAGCTCACATGGCTTCACCGAGAACCAGCTTACCCTTTGGAAGTATCCTTCTATGGTGAAAATGGCAGATCTCAACGGTCACACTTCGAGGGTGATGTATATGGCGCAAAGTCCAGATGGGTGTACAGTGGCATCTGCGGCTGCAGATGAAACTCTTAGATTTTGGAATGTCTTTGGGAGCCCAGCAGAAGCATGCAAAGCCACACCAAAGTCATATAATGAGCCCTTTGCTAATTTCACCCGTATTCGTTGA

Protein sequence

>MS.gene74025.t1

LRAMKGGHRARVGSLSWNNHILTTGGMDGKVVNNDVRVRSHIVETYRGHNQEVCGLKWSPSGQQLSSGGNDNVVHIWDRSVGNLLASGGGGGDHCIKLWNTHTGARLNSVDTGSQVCALLWNQNERELLSSHGFTENQLTLWKYPSMVKMADLNGHTSRVMYMAQSPDGCTVASAAADETLRFWNVFGSPAEACKATPKSYNEPFANFTRIR