AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74351


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74351.t1 MTR_8g045555 90.789 152 14 0 1 152 1 152 5.19e-102 289
MS.gene74351.t1 MTR_8g045520 71.523 151 43 0 1 151 1 151 3.07e-83 241
MS.gene74351.t1 MTR_8g045570 70.395 152 45 0 1 152 1 152 7.04e-81 235
MS.gene74351.t1 MTR_8g045490 71.053 152 44 0 1 152 1 152 2.00e-80 234
MS.gene74351.t1 MTR_8g045400 69.079 152 46 1 1 152 1 151 2.71e-77 226
MS.gene74351.t1 MTR_8g045695 67.763 152 48 1 1 152 1 151 4.02e-76 223
MS.gene74351.t1 MTR_8g045300 64.901 151 53 0 1 151 1 151 1.33e-75 222
MS.gene74351.t1 MTR_8g045735 65.132 152 52 1 1 152 1 151 6.00e-73 216
MS.gene74351.t1 MTR_8g045665 64.474 152 53 1 1 152 1 151 4.60e-72 213
MS.gene74351.t1 MTR_8g045640 64.474 152 53 1 1 152 1 151 2.02e-71 212
MS.gene74351.t1 MTR_8g045560 63.158 152 55 1 1 152 1 151 3.60e-69 206
MS.gene74351.t1 MTR_1g030810 42.484 153 85 2 1 151 1 152 2.04e-40 133
MS.gene74351.t1 MTR_1g030820 36.601 153 94 2 1 151 1 152 9.98e-37 124
MS.gene74351.t1 MTR_1g030840 39.103 156 87 4 1 149 1 155 2.61e-32 113
MS.gene74351.t1 MTR_1g031640 36.486 148 85 4 7 152 7 147 2.35e-28 102
MS.gene74351.t1 MTR_8g012550 33.103 145 94 3 6 149 8 150 1.57e-25 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74351.t1 AT1G70890 34.000 150 96 3 3 150 7 155 1.22e-27 100
MS.gene74351.t1 AT5G28010 34.000 150 96 3 3 150 15 163 3.71e-27 100
MS.gene74351.t1 AT5G28010 34.000 150 96 3 3 150 15 163 3.71e-27 100
MS.gene74351.t1 AT1G70840 32.667 150 98 3 3 150 20 168 4.96e-27 99.8
MS.gene74351.t1 AT1G70830 34.667 150 95 3 3 150 22 170 7.14e-27 99.4
MS.gene74351.t1 AT1G70830 34.667 150 95 3 3 150 22 170 1.50e-26 99.4
MS.gene74351.t1 AT1G70830 34.667 150 95 3 3 150 22 170 5.15e-26 99.4
MS.gene74351.t1 AT1G70830 34.667 150 95 3 3 150 22 170 1.90e-25 99.4
MS.gene74351.t1 AT1G70830 33.333 150 97 3 3 150 184 332 1.03e-23 94.7
MS.gene74351.t1 AT1G70850 32.667 150 98 3 3 150 9 157 1.92e-24 94.7
MS.gene74351.t1 AT1G70830 33.333 150 97 3 3 150 137 285 3.45e-24 95.1
MS.gene74351.t1 AT1G70830 32.258 93 62 1 59 150 31 123 2.20e-14 68.9
MS.gene74351.t1 AT1G70850 32.667 150 98 3 3 150 9 157 5.73e-24 95.1
MS.gene74351.t1 AT1G70850 33.571 140 90 3 13 150 175 313 1.97e-23 93.6
MS.gene74351.t1 AT1G70850 32.667 150 98 3 3 150 9 157 5.73e-24 95.1
MS.gene74351.t1 AT1G70850 33.571 140 90 3 13 150 175 313 1.97e-23 93.6
MS.gene74351.t1 AT1G23130 30.201 149 102 2 3 150 10 157 1.19e-23 90.9
MS.gene74351.t1 AT1G70870 34.437 151 86 4 1 151 1 138 1.92e-21 84.3
MS.gene74351.t1 AT5G28000 28.667 150 104 3 3 150 13 161 1.97e-21 85.1
MS.gene74351.t1 AT1G70880 28.000 150 105 3 3 150 8 156 1.61e-20 82.8
MS.gene74351.t1 AT1G23120 27.083 144 100 3 7 150 7 145 1.36e-19 80.1
MS.gene74351.t1 AT1G23120 27.083 144 100 3 7 150 57 195 5.44e-19 79.7
MS.gene74351.t1 AT1G14930 30.323 155 101 4 1 152 1 151 2.70e-18 77.0
MS.gene74351.t1 AT2G01520 29.677 155 102 4 1 152 1 151 6.13e-16 70.5
MS.gene74351.t1 AT4G23670 30.323 155 101 4 1 152 1 151 6.67e-16 70.5
MS.gene74351.t1 AT2G01530 29.032 155 103 4 1 152 1 151 3.94e-15 68.6
MS.gene74351.t1 AT1G35260 22.517 151 113 3 2 150 1 149 6.63e-15 67.8
MS.gene74351.t1 AT1G24020 29.412 153 104 3 1 151 1 151 8.73e-15 67.8
MS.gene74351.t1 AT1G24020 29.412 153 104 3 1 151 1 151 8.73e-15 67.8
MS.gene74351.t1 AT4G14060 29.677 155 102 4 1 152 1 151 3.98e-14 65.9
MS.gene74351.t1 AT1G14940 27.815 151 106 3 1 150 1 149 7.88e-14 65.1
MS.gene74351.t1 AT4G23680 28.387 155 104 4 1 152 1 151 1.82e-13 64.3
MS.gene74351.t1 AT1G14950 28.758 153 104 4 1 151 1 150 8.11e-13 62.4

Find 29 sgRNAs with CRISPR-Local

Find 83 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGCTCTTCGGTGGAGATAT+TGG 0.246212 8.4:+55643255 MS.gene74351:CDS
AGCTCTTCGGTGGAGATATT+GGG 0.329670 8.4:+55643256 MS.gene74351:CDS
GTCATCCCCTTGATGTAGCT+TGG 0.401893 8.4:-55641744 None:intergenic
AAACATTGGACTTATGTCAT+AGG 0.403651 8.4:+55641788 MS.gene74351:CDS
ATATTCAATAGTCCATTTGA+TGG 0.406324 8.4:-55643340 None:intergenic
TAGAGATATTGATGCTCATC+TGG 0.421031 8.4:+55643425 MS.gene74351:CDS
ACGTAGTCCATCCAGCCATT+AGG 0.444330 8.4:-55643390 None:intergenic
GCAGCAGTGCTGCCATCAAA+TGG 0.452968 8.4:+55643328 MS.gene74351:CDS
AGCTACATCAAGGGGATGAC+TGG 0.453220 8.4:+55641747 MS.gene74351:CDS
GAGGATATTGACCCTCCTAA+TGG 0.460289 8.4:+55643375 MS.gene74351:CDS
GGTAAACTTAGTACTGAACT+TGG 0.476618 8.4:+55641635 MS.gene74351:CDS
ATCTCCACCGAAGAGCTTGT+AGG 0.483925 8.4:-55643250 None:intergenic
ATTCAAATAAATGATAATAG+TGG 0.502034 8.4:+55643306 MS.gene74351:CDS
CTTCCAAGGATGGTGCTCAA+TGG 0.508173 8.4:+55641614 None:intergenic
CATGAAACCAAGCTACATCA+AGG 0.525025 8.4:+55641737 MS.gene74351:CDS
ATATTGACCCTCCTAATGGC+TGG 0.525673 8.4:+55643379 MS.gene74351:CDS
AAACTCACCTACAAGCTCTT+CGG 0.527071 8.4:+55643243 MS.gene74351:CDS
TTTCATGAACTCTTTCACAA+TGG 0.533960 8.4:-55641721 None:intergenic
TAGTCCATCCAGCCATTAGG+AGG 0.553714 8.4:-55643387 None:intergenic
TGACCCTCCTAATGGCTGGA+TGG 0.577019 8.4:+55643383 MS.gene74351:CDS
TGACTGGCATCACACTGATA+CGG 0.601188 8.4:+55641763 MS.gene74351:CDS
GTAAACTTAGTACTGAACTT+GGG 0.605653 8.4:+55641636 MS.gene74351:CDS
ACACTGATACGGTTAAACAT+TGG 0.605669 8.4:+55641774 MS.gene74351:CDS
GCTCTTCGGTGGAGATATTG+GGG 0.615379 8.4:+55643257 MS.gene74351:CDS
ATGAAACCAAGCTACATCAA+GGG 0.623559 8.4:+55641738 MS.gene74351:CDS
CTCACCTACAAGCTCTTCGG+TGG 0.628417 8.4:+55643246 MS.gene74351:CDS
TGAAACCAAGCTACATCAAG+GGG 0.635241 8.4:+55641739 MS.gene74351:CDS
AGTCCATCCAGCCATTAGGA+GGG 0.659406 8.4:-55643386 None:intergenic
TGAATATGAGAAGATCAATG+AGG 0.667184 8.4:+55643356 MS.gene74351:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAAGAACAATATTTGT+AGG - chr8.4:55643060-55643079 None:intergenic 15.0%
!! ACGTAAATTGTTAAAATAAA+AGG - chr8.4:55642143-55642162 None:intergenic 15.0%
!! ATTCAAATAAATGATAATAG+TGG + chr8.4:55643306-55643325 MS.gene74351:CDS 15.0%
!!! CTTTCTAATTTTATCATTTT+GGG - chr8.4:55642069-55642088 None:intergenic 15.0%
!!! TAAAATTACTTTAAAACAAC+TGG + chr8.4:55642974-55642993 MS.gene74351:intron 15.0%
!! ATTATTGAAAACGAAAGATA+AGG - chr8.4:55643108-55643127 None:intergenic 20.0%
!! CATATGAAAATAAAATTGTG+TGG - chr8.4:55643140-55643159 None:intergenic 20.0%
!! TATTCTAATTAAGGTCTTTA+AGG + chr8.4:55642015-55642034 MS.gene74351:intron 20.0%
!!! CACACAATTTTATTTTCATA+TGG + chr8.4:55643138-55643157 MS.gene74351:intron 20.0%
!!! GCTTTCTAATTTTATCATTT+TGG - chr8.4:55642070-55642089 None:intergenic 20.0%
!!! TAATTTTATCATTTTGGGAT+AGG - chr8.4:55642064-55642083 None:intergenic 20.0%
! ATATTCAATAGTCCATTTGA+TGG - chr8.4:55643343-55643362 None:intergenic 25.0%
! CTTAAACTAGAAATCTGAAA+AGG + chr8.4:55643017-55643036 MS.gene74351:intron 25.0%
AAAACAACTGGTACAAAACT+TGG + chr8.4:55642986-55643005 MS.gene74351:intron 30.0%
AAACATTGGACTTATGTCAT+AGG + chr8.4:55641788-55641807 MS.gene74351:CDS 30.0%
AAACTTAGAGATCCAACTAA+GGG - chr8.4:55642791-55642810 None:intergenic 30.0%
ACAAGTTAACATTTCCCTTA+GGG + chr8.4:55642338-55642357 MS.gene74351:intron 30.0%
GTGAATAAGATTTGCAGAAT+CGG + chr8.4:55642937-55642956 MS.gene74351:intron 30.0%
TAAACTTAGAGATCCAACTA+AGG - chr8.4:55642792-55642811 None:intergenic 30.0%
TCTTAAGTGTTACACTTTGT+GGG - chr8.4:55642557-55642576 None:intergenic 30.0%
TGAATATGAGAAGATCAATG+AGG + chr8.4:55643356-55643375 MS.gene74351:CDS 30.0%
TTTCATGAACTCTTTCACAA+TGG - chr8.4:55641724-55641743 None:intergenic 30.0%
! GATGAATTGATGATACTATG+AGG - chr8.4:55642477-55642496 None:intergenic 30.0%
! GTAAACTTAGTACTGAACTT+GGG + chr8.4:55641636-55641655 MS.gene74351:CDS 30.0%
! TACAGGTACTATTTTATCGT+TGG - chr8.4:55642622-55642641 None:intergenic 30.0%
! TTGATAGTAAGTAAACGTAG+AGG - chr8.4:55642114-55642133 None:intergenic 30.0%
!! AGTGTTAACTTGTGTCTTTA+GGG + chr8.4:55642176-55642195 MS.gene74351:intron 30.0%
!!! AGTCATGAGAATGACTTTTT+TGG + chr8.4:55642376-55642395 MS.gene74351:intron 30.0%
!!! GATTCTAGTGTGTTTTTTGA+AGG + chr8.4:55642908-55642927 MS.gene74351:intron 30.0%
ACACTGATACGGTTAAACAT+TGG + chr8.4:55641774-55641793 MS.gene74351:CDS 35.0%
AGTAATGCTTTGCAACATCT+TGG - chr8.4:55642648-55642667 None:intergenic 35.0%
ATGAAACCAAGCTACATCAA+GGG + chr8.4:55641738-55641757 MS.gene74351:CDS 35.0%
ATGCCATTGTACAAATAGTG+GGG - chr8.4:55642442-55642461 None:intergenic 35.0%
CTTAAGTGTTACACTTTGTG+GGG - chr8.4:55642556-55642575 None:intergenic 35.0%
GACAAGTTAACATTTCCCTT+AGG + chr8.4:55642337-55642356 MS.gene74351:intron 35.0%
GATGCCATTGTACAAATAGT+GGG - chr8.4:55642443-55642462 None:intergenic 35.0%
GTACAATGGCATCAAAAGTT+TGG + chr8.4:55642450-55642469 MS.gene74351:intron 35.0%
GTCTTAAGTGTTACACTTTG+TGG - chr8.4:55642558-55642577 None:intergenic 35.0%
TAGAGATATTGATGCTCATC+TGG + chr8.4:55643425-55643444 MS.gene74351:CDS 35.0%
TGATGCCATTGTACAAATAG+TGG - chr8.4:55642444-55642463 None:intergenic 35.0%
TGTTACGCTTATAACAAGGT+TGG - chr8.4:55642533-55642552 None:intergenic 35.0%
TTCCCTTAGGGAAAATTCTA+TGG + chr8.4:55642350-55642369 MS.gene74351:intron 35.0%
! CACCATAGAATTTTCCCTAA+GGG - chr8.4:55642355-55642374 None:intergenic 35.0%
! GCTTTTACATGTTCTCTTGT+TGG + chr8.4:55642088-55642107 MS.gene74351:intron 35.0%
! GGTAAACTTAGTACTGAACT+TGG + chr8.4:55641635-55641654 MS.gene74351:CDS 35.0%
! TCACCATAGAATTTTCCCTA+AGG - chr8.4:55642356-55642375 None:intergenic 35.0%
!! CAGTGTTAACTTGTGTCTTT+AGG + chr8.4:55642175-55642194 MS.gene74351:intron 35.0%
!!! GTGTGTTTTTTGAAGGACTA+AGG + chr8.4:55642915-55642934 MS.gene74351:intron 35.0%
AAACTCACCTACAAGCTCTT+CGG + chr8.4:55643243-55643262 MS.gene74351:CDS 40.0%
ACACCCCACTATTTGTACAA+TGG + chr8.4:55642436-55642455 MS.gene74351:intron 40.0%
CATGAAACCAAGCTACATCA+AGG + chr8.4:55641737-55641756 MS.gene74351:CDS 40.0%
TAGAGATCCAACTAAGGGAA+GGG - chr8.4:55642786-55642805 None:intergenic 40.0%
TCATTACCTTGTGTCTTGAG+CGG + chr8.4:55642315-55642334 MS.gene74351:intron 40.0%
TGAAACCAAGCTACATCAAG+GGG + chr8.4:55641739-55641758 MS.gene74351:CDS 40.0%
TTAGAGATCCAACTAAGGGA+AGG - chr8.4:55642787-55642806 None:intergenic 40.0%
TTGCAACATCTTGGACTTAC+AGG - chr8.4:55642639-55642658 None:intergenic 40.0%
!! GTCTTTAAGGAGTACTCAAG+AGG + chr8.4:55642028-55642047 MS.gene74351:intron 40.0%
AAGCTCTTCGGTGGAGATAT+TGG + chr8.4:55643255-55643274 MS.gene74351:CDS 45.0%
AGCTCTTCGGTGGAGATATT+GGG + chr8.4:55643256-55643275 MS.gene74351:CDS 45.0%
ATATTGACCCTCCTAATGGC+TGG + chr8.4:55643379-55643398 MS.gene74351:CDS 45.0%
GAGGATATTGACCCTCCTAA+TGG + chr8.4:55643375-55643394 MS.gene74351:CDS 45.0%
TCTCGTCACATGACAACAGA+TGG + chr8.4:55643168-55643187 MS.gene74351:intron 45.0%
TGACTGGCATCACACTGATA+CGG + chr8.4:55641763-55641782 MS.gene74351:CDS 45.0%
! CTACTAGTTTCTCCTCCTGA+AGG + chr8.4:55642813-55642832 MS.gene74351:intron 45.0%
! GGGGTGTTACGCTTATAACA+AGG - chr8.4:55642537-55642556 None:intergenic 45.0%
!! TATAAAAAATATTCTAATTA+AGG + chr8.4:55642006-55642025 MS.gene74351:intron 5.0%
!!! AATTGAATTTAAAAAATTTT+AGG - chr8.4:55641892-55641911 None:intergenic 5.0%
ACGTAGTCCATCCAGCCATT+AGG - chr8.4:55643393-55643412 None:intergenic 50.0%
ACTTGTCCGCTCAAGACACA+AGG - chr8.4:55642324-55642343 None:intergenic 50.0%
AGAAGCTCCCTTCCCTTAGT+TGG + chr8.4:55642776-55642795 MS.gene74351:intron 50.0%
AGCTACATCAAGGGGATGAC+TGG + chr8.4:55641747-55641766 MS.gene74351:CDS 50.0%
AGTCCATCCAGCCATTAGGA+GGG - chr8.4:55643389-55643408 None:intergenic 50.0%
ATCTCCACCGAAGAGCTTGT+AGG - chr8.4:55643253-55643272 None:intergenic 50.0%
GAATCGAACCTGAGACCTTC+AGG - chr8.4:55642831-55642850 None:intergenic 50.0%
GCTCTTCGGTGGAGATATTG+GGG + chr8.4:55643257-55643276 MS.gene74351:CDS 50.0%
GTCATCCCCTTGATGTAGCT+TGG - chr8.4:55641747-55641766 None:intergenic 50.0%
TAGTCCATCCAGCCATTAGG+AGG - chr8.4:55643390-55643409 None:intergenic 50.0%
TTTCTCCTCCTGAAGGTCTC+AGG + chr8.4:55642820-55642839 MS.gene74351:intron 50.0%
CTCACCTACAAGCTCTTCGG+TGG + chr8.4:55643246-55643265 MS.gene74351:CDS 55.0%
GCAGCAGTGCTGCCATCAAA+TGG + chr8.4:55643328-55643347 MS.gene74351:CDS 55.0%
GGAGCTTCTACAGTGCAGTC+AGG - chr8.4:55642765-55642784 None:intergenic 55.0%
TCGAACCTGAGACCTTCAGG+AGG - chr8.4:55642828-55642847 None:intergenic 55.0%
TGACCCTCCTAATGGCTGGA+TGG + chr8.4:55643383-55643402 MS.gene74351:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 55641623 55643458 55641623 ID=MS.gene74351
chr8.4 mRNA 55641623 55643458 55641623 ID=MS.gene74351.t1;Parent=MS.gene74351
chr8.4 exon 55641623 55641809 55641623 ID=MS.gene74351.t1.exon1;Parent=MS.gene74351.t1
chr8.4 CDS 55641623 55641809 55641623 ID=cds.MS.gene74351.t1;Parent=MS.gene74351.t1
chr8.4 exon 55643187 55643458 55643187 ID=MS.gene74351.t1.exon2;Parent=MS.gene74351.t1
chr8.4 CDS 55643187 55643458 55643187 ID=cds.MS.gene74351.t1;Parent=MS.gene74351.t1
Gene Sequence

>MS.gene74351

ATGGTGCTCAATGGTAAACTTAGTACTGAACTTGGGATCAAAGCATCTGTTGACAAGTTCTACAATTTCTTTGCAACACAACTTCACGACATGCAAATCCATTGTGAAAGAGTTCATGAAACCAAGCTACATCAAGGGGATGACTGGCATCACACTGATACGGTTAAACATTGGACTTATGTCATAGGTAATCATGTTTCTAACAATTAACTTTTAATTGATTATAAAAAATAAGTAAATATGATATCAACATAAATTTTGTTTCTCCTAAAATTTTTTAAATTCAATTTATAATTAAATATTCTATTAACGTTTGAATTTAGAAATTATTTATTTACAAAGACAAATATCACAATTTTCAATATATTAAATACACTAGTTTTTATAAAAAATATTCTAATTAAGGTCTTTAAGGAGTACTCAAGAGGCATCAGTTAACCTATCCCAAAATGATAAAATTAGAAAGCTTTTACATGTTCTCTTGTTGGCCTCTACGTTTACTTACTATCAAATAAATCCTTTTATTTTAACAATTTACGTACTTTATTAGAGCAGTGTTAACTTGTGTCTTTAGGGCACATGTTAAAAACTTAAAAAAGAAATATTCTCTAAAATTTTGTGCATTTAATTGTTTATAAAATTAAAGATTAAATGCAATGCACACATTTCAATAAATTATTTCTATATTTGAATCATTACCTTGTGTCTTGAGCGGACAAGTTAACATTTCCCTTAGGGAAAATTCTATGGTGAAGTCATGAGAATGACTTTTTTGGTGAAGTTATCACTATAACATCAAAAATGTAATGAGTAACACCCCACTATTTGTACAATGGCATCAAAAGTTTGGTCCTCATAGTATCATCAATTCATCAGATTAACAAGACTACACTTAATCTAATTTTATCCAACCTTGTTATAAGCGTAACACCCCACAAAGTGTAACACTTAAGACTATCACATAATATCATCAACTCTGTTTGCATATTAAAGATTCCAACGATAAAATAGTACCTGTAAGTCCAAGATGTTGCAAAGCATTACTAGATGAATTACTATTGATCATTAATCAGTAATCAACAATTTAAAAATGAATCAAACATTAAAATCAAAATTAACTTTTTCAAAAAAATCAGTTTCCTGACTGCACTGTAGAAGCTCCCTTCCCTTAGTTGGATCTCTAAGTTTATCTACTAGTTTCTCCTCCTGAAGGTCTCAGGTTCGATTCTCTCTGATGTCAATTTGAGTGAGTTAATTTAGCTCAAAAAAAAAAGTTTATCTACTAGATTCTAGTGTGTTTTTTGAAGGACTAAGGTGAATAAGATTTGCAGAATCGGTGATGTTTTAGTATTAAAATTACTTTAAAACAACTGGTACAAAACTTGGATATATATCTTAAACTAGAAATCTGAAAAGGCAAACTATTTATGTTTACCTACAAATATTGTTCTTAAATTTTTATCTTTGTTTAGAAAAATGCGTCCTTATCTTTCGTTTTCAATAATTCTTTCATACCACACAATTTTATTTTCATATGGTATTAATTCTCGTCACATGACAACAGATGGCAATATACACACATGTCATGAGCGTCTTGAAGAAGTTGATGAACAGAATAAAAAACTCACCTACAAGCTCTTCGGTGGAGATATTGGGGAGAACTATAAGAATTTTAAGCTCATTATTCAAATAAATGATAATAGTGGCAGCAGTGCTGCCATCAAATGGACTATTGAATATGAGAAGATCAATGAGGATATTGACCCTCCTAATGGCTGGATGGACTACGTTGCAAAACTTACTAGAGATATTGATGCTCATCTGGTCAAAGCATAG

Protein sequence

>MS.gene74351.t1

MVLNGKLSTELGIKASVDKFYNFFATQLHDMQIHCERVHETKLHQGDDWHHTDTVKHWTYVIDGNIHTCHERLEEVDEQNKKLTYKLFGGDIGENYKNFKLIIQINDNSGSSAAIKWTIEYEKINEDIDPPNGWMDYVAKLTRDIDAHLVKA