Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74448.t1 | XP_003618833.1 | 94.1 | 269 | 16 | 0 | 1 | 269 | 1 | 269 | 2.50E-135 | 491.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74448.t1 | Q5C9I9 | 56.5 | 248 | 108 | 0 | 12 | 259 | 6 | 253 | 1.3e-68 | 261.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74448.t1 | G7KNM8 | 94.1 | 269 | 16 | 0 | 1 | 269 | 1 | 269 | 1.8e-135 | 491.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene74448 | 0.822547 | 2.23E-53 | -1.69E-46 |
| MS.gene050584 | MS.gene74448 | 0.832675 | 8.26E-56 | -1.69E-46 |
| MS.gene05143 | MS.gene74448 | 0.811291 | 7.54E-51 | -1.69E-46 |
| MS.gene052142 | MS.gene74448 | 0.858857 | 6.18E-63 | -1.69E-46 |
| MS.gene052187 | MS.gene74448 | 0.805995 | 1.02E-49 | -1.69E-46 |
| MS.gene053074 | MS.gene74448 | 0.852957 | 3.28E-61 | -1.69E-46 |
| MS.gene053383 | MS.gene74448 | 0.816976 | 4.19E-52 | -1.69E-46 |
| MS.gene05456 | MS.gene74448 | 0.801379 | 9.26E-49 | -1.69E-46 |
| MS.gene055359 | MS.gene74448 | 0.848164 | 7.30E-60 | -1.69E-46 |
| MS.gene058168 | MS.gene74448 | 0.80336 | 3.62E-49 | -1.69E-46 |
| MS.gene059006 | MS.gene74448 | 0.85951 | 3.94E-63 | -1.69E-46 |
| MS.gene059632 | MS.gene74448 | 0.855587 | 5.71E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74448.t1 | MTR_6g023610 | 94.052 | 269 | 16 | 0 | 1 | 269 | 1 | 269 | 0.0 | 516 |
| MS.gene74448.t1 | MTR_6g023910 | 79.926 | 269 | 54 | 0 | 1 | 269 | 1 | 269 | 5.09e-160 | 445 |
| MS.gene74448.t1 | MTR_6g023590 | 69.434 | 265 | 81 | 0 | 1 | 265 | 1 | 265 | 2.61e-137 | 387 |
| MS.gene74448.t1 | MTR_6g023600 | 70.566 | 265 | 78 | 0 | 1 | 265 | 1 | 265 | 2.37e-134 | 380 |
| MS.gene74448.t1 | MTR_6g023990 | 68.797 | 266 | 82 | 1 | 1 | 265 | 1 | 266 | 1.15e-133 | 378 |
| MS.gene74448.t1 | MTR_6g023950 | 71.264 | 261 | 75 | 0 | 1 | 261 | 1 | 261 | 5.65e-129 | 366 |
| MS.gene74448.t1 | MTR_6g023600 | 69.057 | 265 | 76 | 1 | 1 | 265 | 1 | 259 | 5.44e-128 | 363 |
| MS.gene74448.t1 | MTR_7g110130 | 50.400 | 250 | 123 | 1 | 12 | 261 | 5 | 253 | 8.12e-81 | 244 |
| MS.gene74448.t1 | MTR_2g090165 | 50.000 | 250 | 123 | 2 | 12 | 261 | 5 | 252 | 1.52e-77 | 236 |
| MS.gene74448.t1 | MTR_7g110140 | 48.606 | 251 | 127 | 2 | 11 | 261 | 4 | 252 | 3.50e-75 | 230 |
| MS.gene74448.t1 | MTR_7g110160 | 47.600 | 250 | 111 | 2 | 12 | 261 | 5 | 234 | 2.90e-71 | 219 |
| MS.gene74448.t1 | MTR_7g105460 | 42.912 | 261 | 147 | 2 | 1 | 261 | 1 | 259 | 1.80e-68 | 213 |
| MS.gene74448.t1 | MTR_4g052320 | 44.488 | 254 | 137 | 3 | 8 | 261 | 3 | 252 | 1.59e-65 | 205 |
| MS.gene74448.t1 | MTR_4g052300 | 45.238 | 252 | 133 | 3 | 8 | 259 | 3 | 249 | 2.24e-65 | 205 |
| MS.gene74448.t1 | MTR_6g035395 | 45.600 | 250 | 121 | 3 | 12 | 261 | 4 | 238 | 4.29e-65 | 204 |
| MS.gene74448.t1 | MTR_4g052340 | 44.578 | 249 | 133 | 3 | 12 | 260 | 13 | 256 | 9.85e-65 | 203 |
| MS.gene74448.t1 | MTR_6g023940 | 54.673 | 214 | 35 | 3 | 1 | 214 | 1 | 152 | 3.56e-64 | 198 |
| MS.gene74448.t1 | MTR_4g052350 | 44.269 | 253 | 136 | 3 | 12 | 264 | 13 | 260 | 2.46e-63 | 200 |
| MS.gene74448.t1 | MTR_4g078630 | 45.098 | 255 | 129 | 4 | 12 | 259 | 18 | 268 | 6.55e-63 | 199 |
| MS.gene74448.t1 | MTR_7g071680 | 42.322 | 267 | 132 | 6 | 12 | 259 | 28 | 291 | 2.37e-59 | 191 |
| MS.gene74448.t1 | MTR_7g107670 | 38.722 | 266 | 153 | 5 | 6 | 265 | 26 | 287 | 3.80e-57 | 185 |
| MS.gene74448.t1 | MTR_7g083870 | 40.000 | 265 | 144 | 7 | 6 | 261 | 28 | 286 | 4.05e-57 | 185 |
| MS.gene74448.t1 | MTR_7g105590 | 45.026 | 191 | 103 | 2 | 10 | 200 | 5 | 193 | 4.32e-52 | 169 |
| MS.gene74448.t1 | MTR_7g105580 | 39.600 | 250 | 131 | 3 | 12 | 261 | 7 | 236 | 1.70e-50 | 166 |
| MS.gene74448.t1 | MTR_3g020670 | 36.823 | 277 | 156 | 5 | 4 | 268 | 7 | 276 | 7.06e-49 | 163 |
| MS.gene74448.t1 | MTR_3g113870 | 39.535 | 215 | 114 | 6 | 21 | 231 | 29 | 231 | 8.28e-45 | 151 |
| MS.gene74448.t1 | MTR_4g084800 | 50.714 | 140 | 68 | 1 | 1 | 140 | 1 | 139 | 3.47e-44 | 147 |
| MS.gene74448.t1 | MTR_7g105570 | 34.137 | 249 | 111 | 2 | 13 | 261 | 21 | 216 | 1.83e-36 | 129 |
| MS.gene74448.t1 | MTR_1g082760 | 33.208 | 265 | 150 | 8 | 12 | 262 | 39 | 290 | 5.09e-28 | 109 |
| MS.gene74448.t1 | MTR_7g113650 | 31.818 | 264 | 155 | 8 | 12 | 262 | 39 | 290 | 5.25e-28 | 109 |
| MS.gene74448.t1 | MTR_7g094510 | 31.008 | 258 | 166 | 5 | 11 | 262 | 4 | 255 | 1.31e-27 | 107 |
| MS.gene74448.t1 | MTR_7g094520 | 31.439 | 264 | 171 | 5 | 4 | 262 | 2 | 260 | 1.74e-26 | 104 |
| MS.gene74448.t1 | MTR_4g132850 | 31.873 | 251 | 158 | 6 | 12 | 259 | 6 | 246 | 4.84e-26 | 103 |
| MS.gene74448.t1 | MTR_3g085740 | 30.370 | 270 | 166 | 9 | 1 | 262 | 64 | 319 | 5.59e-23 | 96.3 |
| MS.gene74448.t1 | MTR_4g131740 | 30.418 | 263 | 155 | 8 | 12 | 259 | 39 | 288 | 2.53e-21 | 91.3 |
| MS.gene74448.t1 | MTR_4g058840 | 28.740 | 254 | 154 | 8 | 13 | 251 | 11 | 252 | 3.94e-20 | 87.4 |
| MS.gene74448.t1 | MTR_4g058820 | 28.740 | 254 | 154 | 8 | 13 | 251 | 11 | 252 | 3.94e-20 | 87.4 |
| MS.gene74448.t1 | MTR_4g064887 | 29.924 | 264 | 173 | 8 | 1 | 259 | 1 | 257 | 4.00e-20 | 87.4 |
| MS.gene74448.t1 | MTR_4g064887 | 31.455 | 213 | 137 | 6 | 1 | 208 | 1 | 209 | 1.63e-18 | 82.0 |
| MS.gene74448.t1 | MTR_4g058810 | 26.908 | 249 | 165 | 6 | 13 | 251 | 11 | 252 | 2.36e-18 | 82.8 |
| MS.gene74448.t1 | MTR_1196s0010 | 31.660 | 259 | 144 | 10 | 16 | 262 | 277 | 514 | 6.79e-18 | 83.2 |
| MS.gene74448.t1 | MTR_1g051165 | 26.357 | 258 | 176 | 7 | 12 | 258 | 13 | 267 | 8.37e-17 | 78.6 |
| MS.gene74448.t1 | MTR_1g068955 | 29.961 | 257 | 165 | 6 | 11 | 259 | 12 | 261 | 1.30e-16 | 78.2 |
| MS.gene74448.t1 | MTR_7g105560 | 59.701 | 67 | 26 | 1 | 1 | 67 | 1 | 66 | 6.95e-16 | 73.2 |
| MS.gene74448.t1 | MTR_7g063320 | 29.688 | 256 | 168 | 7 | 13 | 262 | 15 | 264 | 2.19e-15 | 74.3 |
| MS.gene74448.t1 | MTR_7g063330 | 27.165 | 254 | 174 | 7 | 13 | 261 | 15 | 262 | 3.95e-15 | 73.6 |
| MS.gene74448.t1 | MTR_8g096580 | 28.251 | 223 | 136 | 7 | 16 | 232 | 48 | 252 | 4.18e-15 | 74.3 |
| MS.gene74448.t1 | MTR_3g068085 | 28.626 | 262 | 169 | 9 | 13 | 267 | 15 | 265 | 1.45e-14 | 72.0 |
| MS.gene74448.t1 | MTR_7g063270 | 27.203 | 261 | 165 | 8 | 13 | 261 | 16 | 263 | 2.41e-14 | 71.2 |
| MS.gene74448.t1 | MTR_3g068175 | 28.782 | 271 | 163 | 9 | 3 | 259 | 5 | 259 | 2.77e-14 | 71.2 |
| MS.gene74448.t1 | MTR_7g063260 | 27.059 | 255 | 174 | 7 | 13 | 261 | 16 | 264 | 4.31e-14 | 70.9 |
| MS.gene74448.t1 | MTR_7g063390 | 26.740 | 273 | 179 | 9 | 1 | 261 | 1 | 264 | 6.64e-13 | 67.4 |
| MS.gene74448.t1 | MTR_8g096620 | 27.551 | 196 | 132 | 5 | 13 | 204 | 45 | 234 | 8.47e-13 | 67.8 |
| MS.gene74448.t1 | MTR_3g068175 | 28.364 | 275 | 163 | 10 | 3 | 259 | 5 | 263 | 2.62e-12 | 65.9 |
| MS.gene74448.t1 | MTR_4g086400 | 27.962 | 211 | 128 | 6 | 14 | 207 | 5 | 208 | 2.91e-12 | 66.6 |
| MS.gene74448.t1 | MTR_8g044240 | 26.106 | 226 | 148 | 6 | 5 | 218 | 61 | 279 | 4.51e-12 | 65.5 |
| MS.gene74448.t1 | MTR_4g134660 | 25.728 | 206 | 148 | 5 | 10 | 212 | 3 | 206 | 6.12e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene74448.t1 | AT2G47140 | 50.198 | 253 | 124 | 2 | 9 | 261 | 2 | 252 | 1.53e-82 | 248 |
| MS.gene74448.t1 | AT2G47130 | 48.438 | 256 | 123 | 4 | 9 | 261 | 2 | 251 | 5.04e-76 | 232 |
| MS.gene74448.t1 | AT3G29260 | 49.802 | 253 | 124 | 3 | 9 | 261 | 2 | 251 | 2.49e-75 | 230 |
| MS.gene74448.t1 | AT2G47120 | 46.640 | 253 | 131 | 4 | 9 | 261 | 2 | 250 | 2.18e-73 | 225 |
| MS.gene74448.t1 | AT3G29250 | 45.914 | 257 | 137 | 2 | 5 | 261 | 34 | 288 | 2.12e-72 | 224 |
| MS.gene74448.t1 | AT3G29250 | 46.245 | 253 | 134 | 2 | 9 | 261 | 2 | 252 | 2.56e-72 | 223 |
| MS.gene74448.t1 | AT2G47120 | 46.400 | 250 | 130 | 4 | 12 | 261 | 6 | 251 | 9.25e-72 | 221 |
| MS.gene74448.t1 | AT3G29250 | 45.560 | 259 | 137 | 3 | 5 | 261 | 34 | 290 | 1.99e-70 | 219 |
| MS.gene74448.t1 | AT3G51680 | 42.960 | 277 | 136 | 5 | 1 | 259 | 20 | 292 | 8.74e-62 | 197 |
| MS.gene74448.t1 | AT3G26760 | 40.840 | 262 | 144 | 6 | 12 | 265 | 35 | 293 | 2.71e-57 | 185 |
| MS.gene74448.t1 | AT3G26770 | 39.300 | 257 | 141 | 6 | 12 | 259 | 40 | 290 | 2.35e-54 | 178 |
| MS.gene74448.t1 | AT4G03140 | 38.431 | 255 | 149 | 4 | 12 | 261 | 77 | 328 | 1.39e-51 | 172 |
| MS.gene74448.t1 | AT1G52340 | 40.000 | 260 | 144 | 5 | 12 | 261 | 17 | 274 | 1.99e-51 | 170 |
| MS.gene74448.t1 | AT3G42960 | 39.924 | 263 | 144 | 6 | 8 | 261 | 3 | 260 | 5.46e-49 | 163 |
| MS.gene74448.t1 | AT5G18210 | 32.946 | 258 | 160 | 5 | 13 | 262 | 8 | 260 | 2.00e-28 | 109 |
| MS.gene74448.t1 | AT5G18210 | 34.855 | 241 | 144 | 5 | 13 | 245 | 8 | 243 | 3.30e-27 | 106 |
| MS.gene74448.t1 | AT3G03980 | 31.868 | 273 | 165 | 6 | 4 | 262 | 5 | 270 | 1.02e-26 | 105 |
| MS.gene74448.t1 | AT3G05260 | 34.434 | 212 | 124 | 5 | 2 | 203 | 24 | 230 | 2.18e-26 | 104 |
| MS.gene74448.t1 | AT3G04000 | 33.333 | 264 | 157 | 6 | 1 | 250 | 1 | 259 | 2.39e-26 | 104 |
| MS.gene74448.t1 | AT1G54870 | 34.500 | 200 | 119 | 4 | 12 | 203 | 83 | 278 | 2.49e-26 | 105 |
| MS.gene74448.t1 | AT1G54870 | 34.500 | 200 | 119 | 4 | 12 | 203 | 81 | 276 | 2.99e-26 | 105 |
| MS.gene74448.t1 | AT2G47150 | 33.068 | 251 | 109 | 9 | 13 | 261 | 1 | 194 | 1.30e-25 | 100 |
| MS.gene74448.t1 | AT4G05530 | 31.325 | 249 | 162 | 6 | 12 | 259 | 8 | 248 | 8.84e-21 | 89.0 |
| MS.gene74448.t1 | AT4G13180 | 26.937 | 271 | 180 | 6 | 1 | 262 | 1 | 262 | 1.22e-20 | 89.0 |
| MS.gene74448.t1 | AT1G24360 | 27.237 | 257 | 164 | 7 | 12 | 259 | 73 | 315 | 2.56e-19 | 86.3 |
| MS.gene74448.t1 | AT1G63380 | 27.490 | 251 | 162 | 9 | 13 | 251 | 17 | 259 | 2.34e-18 | 82.8 |
| MS.gene74448.t1 | AT1G63380 | 27.490 | 251 | 162 | 9 | 13 | 251 | 15 | 257 | 2.35e-18 | 82.8 |
| MS.gene74448.t1 | AT1G63380 | 27.490 | 251 | 162 | 9 | 13 | 251 | 19 | 261 | 2.53e-18 | 82.8 |
| MS.gene74448.t1 | AT1G63380 | 27.490 | 251 | 162 | 9 | 13 | 251 | 22 | 264 | 3.35e-18 | 82.4 |
| MS.gene74448.t1 | AT1G62610 | 27.236 | 246 | 169 | 7 | 13 | 251 | 13 | 255 | 1.03e-17 | 80.9 |
| MS.gene74448.t1 | AT1G62610 | 27.236 | 246 | 169 | 7 | 13 | 251 | 15 | 257 | 1.06e-17 | 80.9 |
| MS.gene74448.t1 | AT1G62610 | 27.236 | 246 | 169 | 7 | 13 | 251 | 14 | 256 | 1.08e-17 | 80.9 |
| MS.gene74448.t1 | AT1G62610 | 27.236 | 246 | 169 | 7 | 13 | 251 | 19 | 261 | 1.26e-17 | 80.9 |
| MS.gene74448.t1 | AT3G46170 | 26.577 | 222 | 150 | 5 | 12 | 221 | 25 | 245 | 7.52e-17 | 79.0 |
| MS.gene74448.t1 | AT3G47350 | 26.768 | 198 | 131 | 6 | 13 | 204 | 44 | 233 | 1.56e-15 | 75.5 |
| MS.gene74448.t1 | AT3G47350 | 26.768 | 198 | 131 | 6 | 13 | 204 | 44 | 233 | 1.98e-15 | 75.1 |
| MS.gene74448.t1 | AT5G06060 | 27.174 | 276 | 155 | 8 | 7 | 261 | 3 | 253 | 2.38e-15 | 74.3 |
| MS.gene74448.t1 | AT3G55310 | 27.232 | 224 | 144 | 7 | 13 | 221 | 17 | 236 | 3.17e-15 | 74.3 |
| MS.gene74448.t1 | AT3G55290 | 26.577 | 222 | 148 | 6 | 13 | 221 | 18 | 237 | 6.14e-15 | 73.2 |
| MS.gene74448.t1 | AT3G55290 | 26.577 | 222 | 148 | 6 | 13 | 221 | 17 | 236 | 6.77e-15 | 73.2 |
| MS.gene74448.t1 | AT3G47360 | 28.283 | 198 | 128 | 6 | 13 | 204 | 45 | 234 | 8.82e-15 | 73.2 |
| MS.gene74448.t1 | AT5G50690 | 28.889 | 180 | 113 | 6 | 12 | 184 | 44 | 215 | 8.86e-15 | 72.4 |
| MS.gene74448.t1 | AT5G50590 | 28.889 | 180 | 113 | 6 | 12 | 184 | 44 | 215 | 8.86e-15 | 72.4 |
| MS.gene74448.t1 | AT5G50600 | 25.773 | 194 | 138 | 4 | 13 | 204 | 45 | 234 | 8.91e-15 | 73.6 |
| MS.gene74448.t1 | AT5G50700 | 25.773 | 194 | 138 | 4 | 13 | 204 | 45 | 234 | 8.91e-15 | 73.6 |
| MS.gene74448.t1 | AT2G29370 | 27.519 | 258 | 179 | 6 | 13 | 268 | 16 | 267 | 1.18e-14 | 72.4 |
| MS.gene74448.t1 | AT5G50690 | 28.492 | 179 | 115 | 6 | 12 | 184 | 44 | 215 | 1.39e-14 | 72.4 |
| MS.gene74448.t1 | AT5G50590 | 28.492 | 179 | 115 | 6 | 12 | 184 | 44 | 215 | 1.39e-14 | 72.4 |
| MS.gene74448.t1 | AT5G50770 | 30.481 | 187 | 122 | 5 | 13 | 196 | 45 | 226 | 1.67e-14 | 72.8 |
| MS.gene74448.t1 | AT2G29340 | 27.381 | 252 | 169 | 6 | 13 | 259 | 7 | 249 | 2.27e-14 | 71.6 |
| MS.gene74448.t1 | AT2G29360 | 27.308 | 260 | 177 | 7 | 13 | 268 | 16 | 267 | 3.63e-14 | 71.2 |
| MS.gene74448.t1 | AT2G29340 | 27.092 | 251 | 171 | 6 | 13 | 259 | 7 | 249 | 3.75e-14 | 71.6 |
| MS.gene74448.t1 | AT2G29340 | 27.861 | 201 | 134 | 5 | 13 | 208 | 7 | 201 | 5.63e-13 | 66.6 |
| MS.gene74448.t1 | AT2G29340 | 27.861 | 201 | 134 | 5 | 13 | 208 | 7 | 201 | 5.63e-13 | 66.6 |
| MS.gene74448.t1 | AT2G29310 | 26.866 | 268 | 164 | 11 | 13 | 266 | 7 | 256 | 7.08e-13 | 67.4 |
| MS.gene74448.t1 | AT2G17845 | 27.670 | 206 | 137 | 6 | 13 | 208 | 47 | 250 | 3.44e-12 | 65.9 |
| MS.gene74448.t1 | AT2G29300 | 27.106 | 273 | 152 | 11 | 13 | 263 | 7 | 254 | 3.92e-12 | 65.5 |
| MS.gene74448.t1 | AT2G29310 | 29.048 | 210 | 120 | 9 | 13 | 208 | 7 | 201 | 1.26e-11 | 62.8 |
| MS.gene74448.t1 | AT2G29320 | 27.308 | 260 | 160 | 10 | 13 | 259 | 13 | 256 | 5.29e-11 | 62.0 |
Find 75 sgRNAs with CRISPR-Local
Find 91 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTCACCCTTATCCCATATT+TGG | 0.193090 | 6.4:+122721 | None:intergenic |
| TCCATCTGATCAGACTATTT+TGG | 0.219303 | 6.4:-122966 | MS.gene74448:CDS |
| CAATTTACGGTCAATGCTTA+TGG | 0.226097 | 6.4:-123676 | MS.gene74448:CDS |
| ATATCGAAGCATGCAATAAT+AGG | 0.242360 | 6.4:-122773 | MS.gene74448:CDS |
| GGCTGATGCTGTGTTGTTCT+TGG | 0.253786 | 6.4:-122570 | MS.gene74448:CDS |
| CATCTACTAACAACGGTCTT+AGG | 0.262828 | 6.4:-123909 | MS.gene74448:CDS |
| CCATTGACTATGAGATTGTT+AGG | 0.292303 | 6.4:-122671 | MS.gene74448:CDS |
| GTGGATGGCAGCTATGTTTA+TGG | 0.299988 | 6.4:-122506 | MS.gene74448:CDS |
| AGTGTGCAACTTGCCAAATA+TGG | 0.304782 | 6.4:-122734 | MS.gene74448:CDS |
| CACCATTCGTGCACCTTGTT+CGG | 0.349762 | 6.4:+123816 | None:intergenic |
| GATATTATGTTTAGCAATGC+TGG | 0.362324 | 6.4:-122998 | MS.gene74448:CDS |
| GCCAATGCCGCTGGCACCTC+CGG | 0.364087 | 6.4:+123861 | None:intergenic |
| CGTCTCTTTGCCAATGCCGC+TGG | 0.394229 | 6.4:+123852 | None:intergenic |
| GAGCGAGTGGCTGCATCCAT+TGG | 0.421907 | 6.4:-123763 | MS.gene74448:CDS |
| CGCGGCCCGTGCGATGGTGG+AGG | 0.424217 | 6.4:-122873 | MS.gene74448:CDS |
| ACCGGAGGTGCCAGCGGCAT+TGG | 0.425693 | 6.4:-123862 | MS.gene74448:CDS |
| AATGGTGTTGCTAATCCATT+TGG | 0.430166 | 6.4:+122689 | None:intergenic |
| AACTTGCCAAATATGGGATA+AGG | 0.435956 | 6.4:-122727 | MS.gene74448:CDS |
| TATCGAAGCATGCAATAATA+GGG | 0.448557 | 6.4:-122772 | MS.gene74448:CDS |
| GTGAATTGTGTCTCGCCAAA+TGG | 0.451124 | 6.4:-122704 | MS.gene74448:CDS |
| TGTGTGCACAGCCAGCGTCC+TGG | 0.454120 | 6.4:-122831 | MS.gene74448:CDS |
| CGATGGTGGAGGGGCACGTG+AGG | 0.459948 | 6.4:-122862 | MS.gene74448:CDS |
| ACTTGCCAAATATGGGATAA+GGG | 0.469695 | 6.4:-122726 | MS.gene74448:CDS |
| TGCTGACATTCAAGACGAGT+TGG | 0.475276 | 6.4:-123788 | MS.gene74448:CDS |
| GTGTTTGCAGTTAACATTCG+AGG | 0.478434 | 6.4:-122917 | MS.gene74448:CDS |
| AATGGATGAAGGTGCTCCTA+TGG | 0.481454 | 6.4:+123737 | None:intergenic |
| GCTGACATTCAAGACGAGTT+GGG | 0.485584 | 6.4:-123787 | MS.gene74448:CDS |
| CATTGACTATGAGATTGTTA+GGG | 0.487258 | 6.4:-122670 | MS.gene74448:CDS |
| ATGGTGGAGGGGCACGTGAG+GGG | 0.487557 | 6.4:-122860 | MS.gene74448:CDS |
| GGTGACGATGGCTACTTTGC+CGG | 0.490347 | 6.4:+123882 | None:intergenic |
| GTGTGCAACTTGCCAAATAT+GGG | 0.491153 | 6.4:-122733 | MS.gene74448:CDS |
| TACGGTCAATGCTTATGGAC+AGG | 0.491275 | 6.4:-123671 | MS.gene74448:intron |
| CTGCATCCATTGGCGGCCAT+AGG | 0.498803 | 6.4:-123753 | MS.gene74448:CDS |
| GTGTGCACAGCCAGCGTCCT+GGG | 0.501862 | 6.4:-122830 | MS.gene74448:CDS |
| GATGGTGGAGGGGCACGTGA+GGG | 0.504325 | 6.4:-122861 | MS.gene74448:CDS |
| GAAATACGCGGCCCGTGCGA+TGG | 0.514059 | 6.4:-122879 | MS.gene74448:CDS |
| GCGGCCCGTGCGATGGTGGA+GGG | 0.515052 | 6.4:-122872 | MS.gene74448:CDS |
| CCTAACAATCTCATAGTCAA+TGG | 0.517810 | 6.4:+122671 | None:intergenic |
| ATATTATGTTTAGCAATGCT+GGG | 0.517928 | 6.4:-122997 | MS.gene74448:CDS |
| TTATGAACAAAACAAAAGGT+TGG | 0.519972 | 6.4:-122621 | MS.gene74448:CDS |
| AACAACGGTCTTAGGTTAGC+CGG | 0.520911 | 6.4:-123901 | MS.gene74448:CDS |
| TCCAAAATAGTCTGATCAGA+TGG | 0.534810 | 6.4:+122965 | None:intergenic |
| GGCAAAGTAGCCATCGTCAC+CGG | 0.538495 | 6.4:-123880 | MS.gene74448:CDS |
| TGATTTATGAACAAAACAAA+AGG | 0.539286 | 6.4:-122625 | MS.gene74448:CDS |
| AAACAAAAGGTTGGAAGGAG+TGG | 0.540677 | 6.4:-122612 | MS.gene74448:CDS |
| ATACGCGGCCCGTGCGATGG+TGG | 0.544535 | 6.4:-122876 | MS.gene74448:CDS |
| GAACAAAACAAAAGGTTGGA+AGG | 0.552336 | 6.4:-122617 | MS.gene74448:CDS |
| AGACGAGTTGGGCGAGCGAG+TGG | 0.553364 | 6.4:-123776 | MS.gene74448:CDS |
| GCACATCTCTTTGCCGAACA+AGG | 0.555212 | 6.4:-123829 | MS.gene74448:CDS |
| CGTGCCCCTCCACCATCGCA+CGG | 0.557137 | 6.4:+122867 | None:intergenic |
| TGCCGAACAAGGTGCACGAA+TGG | 0.557302 | 6.4:-123818 | MS.gene74448:CDS |
| GCCAGCGTCCTGGGTAGCGA+AGG | 0.561660 | 6.4:-122821 | MS.gene74448:CDS |
| CGAGTGGCTGCATCCATTGG+CGG | 0.561662 | 6.4:-123760 | MS.gene74448:CDS |
| CAGCGGCATTGGCAAAGAGA+CGG | 0.569942 | 6.4:-123851 | MS.gene74448:CDS |
| CGAACAAGGTGCACGAATGG+TGG | 0.575204 | 6.4:-123815 | MS.gene74448:CDS |
| GACATTGATCTTCGTGTGGA+TGG | 0.580099 | 6.4:-122521 | MS.gene74448:CDS |
| TCAAGACACCTTCGCTACCC+AGG | 0.581633 | 6.4:+122813 | None:intergenic |
| GCTGGCACCTCCGGTGACGA+TGG | 0.584463 | 6.4:+123870 | None:intergenic |
| ATCGTCACCGGAGGTGCCAG+CGG | 0.590387 | 6.4:-123868 | MS.gene74448:CDS |
| TGCTATGTCACAATGGATGA+AGG | 0.590504 | 6.4:+123726 | None:intergenic |
| CGGCCCGTGCGATGGTGGAG+GGG | 0.595105 | 6.4:-122871 | MS.gene74448:CDS |
| GGTGCTCCTATGGCCGCCAA+TGG | 0.595669 | 6.4:+123747 | None:intergenic |
| AATAATAGGGTTGATGCGCT+CGG | 0.601962 | 6.4:-122759 | MS.gene74448:CDS |
| GTGCCCCTCCACCATCGCAC+GGG | 0.603060 | 6.4:+122868 | None:intergenic |
| ACCTTCGCTACCCAGGACGC+TGG | 0.605739 | 6.4:+122820 | None:intergenic |
| CACTGACATTGATCTTCGTG+TGG | 0.608759 | 6.4:-122525 | MS.gene74448:CDS |
| GAATGTTAACTGCAAACACA+TGG | 0.631228 | 6.4:+122921 | None:intergenic |
| GTTAACTGCAAACACATGGT+CGG | 0.632263 | 6.4:+122925 | None:intergenic |
| CTTCATTTGCTATGTCACAA+TGG | 0.633784 | 6.4:+123719 | None:intergenic |
| AAAGTAGCCATCGTCACCGG+AGG | 0.651370 | 6.4:-123877 | MS.gene74448:CDS |
| GGTTCTTAACACTAAACATG+TGG | 0.655132 | 6.4:-122591 | MS.gene74448:CDS |
| ATTGACTATGAGATTGTTAG+GGG | 0.661906 | 6.4:-122669 | MS.gene74448:CDS |
| GACATTGTGCGTGAAATACG+CGG | 0.664173 | 6.4:-122891 | MS.gene74448:CDS |
| TGGGTAGCGAAGGTGTCTTG+AGG | 0.664401 | 6.4:-122811 | MS.gene74448:CDS |
| GAAGCTTCATCTACTAACAA+CGG | 0.689074 | 6.4:-123916 | MS.gene74448:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATAAAATAATTTTTTTTAT+AGG | - | chr6.4:123219-123238 | MS.gene74448:intron | 0.0% |
| !!! | ATAAAATAATTTTTTTTATA+GGG | - | chr6.4:123220-123239 | MS.gene74448:intron | 0.0% |
| !! | AAAAAATCTCATTCAATTTA+CGG | - | chr6.4:122720-122739 | MS.gene74448:CDS | 15.0% |
| !! | ACATTATAATGTAGAAATTA+TGG | + | chr6.4:123072-123091 | None:intergenic | 15.0% |
| !!! | ATAAAGTCTTATATAGTAAA+TGG | + | chr6.4:123265-123284 | None:intergenic | 15.0% |
| !!! | TAAAGTCTTATATAGTAAAT+GGG | + | chr6.4:123264-123283 | None:intergenic | 15.0% |
| !! | CAAAATTTCATAATACTCTT+AGG | + | chr6.4:122798-122817 | None:intergenic | 20.0% |
| !! | TGATTTATGAACAAAACAAA+AGG | - | chr6.4:123784-123803 | MS.gene74448:CDS | 20.0% |
| !!! | AAAGTCTTATATAGTAAATG+GGG | + | chr6.4:123263-123282 | None:intergenic | 20.0% |
| !!! | GAAAGAAAAAGTTGTATTTT+AGG | + | chr6.4:123375-123394 | None:intergenic | 20.0% |
| !!! | TACTGATAGTATAATTTTGT+AGG | - | chr6.4:123159-123178 | MS.gene74448:intron | 20.0% |
| ! | ATATTATGTTTAGCAATGCT+GGG | - | chr6.4:123412-123431 | MS.gene74448:intron | 25.0% |
| ! | TTATGAACAAAACAAAAGGT+TGG | - | chr6.4:123788-123807 | MS.gene74448:CDS | 25.0% |
| !!! | GAAGAATTTGAAGTTTTTTG+AGG | + | chr6.4:122921-122940 | None:intergenic | 25.0% |
| AACATAATATCTACCTGTTG+TGG | + | chr6.4:123402-123421 | None:intergenic | 30.0% | |
| ATATCGAAGCATGCAATAAT+AGG | - | chr6.4:123636-123655 | MS.gene74448:intron | 30.0% | |
| ATTGACTATGAGATTGTTAG+GGG | - | chr6.4:123740-123759 | MS.gene74448:CDS | 30.0% | |
| GATATTATGTTTAGCAATGC+TGG | - | chr6.4:123411-123430 | MS.gene74448:intron | 30.0% | |
| GGACAGGTAAAAATTAACTA+GGG | - | chr6.4:122754-122773 | MS.gene74448:CDS | 30.0% | |
| TATCGAAGCATGCAATAATA+GGG | - | chr6.4:123637-123656 | MS.gene74448:intron | 30.0% | |
| TGGACAGGTAAAAATTAACT+AGG | - | chr6.4:122753-122772 | MS.gene74448:CDS | 30.0% | |
| ! | CATTGACTATGAGATTGTTA+GGG | - | chr6.4:123739-123758 | MS.gene74448:CDS | 30.0% |
| ! | TAACTTTAATTAGTCTCTGC+TGG | + | chr6.4:123141-123160 | None:intergenic | 30.0% |
| ! | TTTTCTTTCTTTTCCACAAC+AGG | - | chr6.4:123386-123405 | MS.gene74448:intron | 30.0% |
| AACTTGCCAAATATGGGATA+AGG | - | chr6.4:123682-123701 | MS.gene74448:CDS | 35.0% | |
| ACTTGCCAAATATGGGATAA+GGG | - | chr6.4:123683-123702 | MS.gene74448:CDS | 35.0% | |
| ATTCACCCTTATCCCATATT+TGG | + | chr6.4:123691-123710 | None:intergenic | 35.0% | |
| CAATTTACGGTCAATGCTTA+TGG | - | chr6.4:122733-122752 | MS.gene74448:CDS | 35.0% | |
| CCTAACAATCTCATAGTCAA+TGG | + | chr6.4:123741-123760 | None:intergenic | 35.0% | |
| GAACAAAACAAAAGGTTGGA+AGG | - | chr6.4:123792-123811 | MS.gene74448:CDS | 35.0% | |
| GAAGCTTCATCTACTAACAA+CGG | - | chr6.4:122493-122512 | MS.gene74448:CDS | 35.0% | |
| GAATGTTAACTGCAAACACA+TGG | + | chr6.4:123491-123510 | None:intergenic | 35.0% | |
| GGTTCTTAACACTAAACATG+TGG | - | chr6.4:123818-123837 | MS.gene74448:CDS | 35.0% | |
| TCCAAAATAGTCTGATCAGA+TGG | + | chr6.4:123447-123466 | None:intergenic | 35.0% | |
| ! | CCATTGACTATGAGATTGTT+AGG | - | chr6.4:123738-123757 | MS.gene74448:CDS | 35.0% |
| ! | TCCATCTGATCAGACTATTT+TGG | - | chr6.4:123443-123462 | MS.gene74448:intron | 35.0% |
| !! | AATGGTGTTGCTAATCCATT+TGG | + | chr6.4:123723-123742 | None:intergenic | 35.0% |
| !! | CTTCATTTGCTATGTCACAA+TGG | + | chr6.4:122693-122712 | None:intergenic | 35.0% |
| AAACAAAAGGTTGGAAGGAG+TGG | - | chr6.4:123797-123816 | MS.gene74448:CDS | 40.0% | |
| AGTGTGCAACTTGCCAAATA+TGG | - | chr6.4:123675-123694 | MS.gene74448:CDS | 40.0% | |
| CATCTACTAACAACGGTCTT+AGG | - | chr6.4:122500-122519 | MS.gene74448:CDS | 40.0% | |
| GTGTGCAACTTGCCAAATAT+GGG | - | chr6.4:123676-123695 | MS.gene74448:CDS | 40.0% | |
| GTGTTTGCAGTTAACATTCG+AGG | - | chr6.4:123492-123511 | MS.gene74448:intron | 40.0% | |
| GTTAACTGCAAACACATGGT+CGG | + | chr6.4:123487-123506 | None:intergenic | 40.0% | |
| TGCTATGTCACAATGGATGA+AGG | + | chr6.4:122686-122705 | None:intergenic | 40.0% | |
| !! | AATAATAGGGTTGATGCGCT+CGG | - | chr6.4:123650-123669 | MS.gene74448:intron | 40.0% |
| AACAACGGTCTTAGGTTAGC+CGG | - | chr6.4:122508-122527 | MS.gene74448:CDS | 45.0% | |
| CACTGACATTGATCTTCGTG+TGG | - | chr6.4:123884-123903 | MS.gene74448:CDS | 45.0% | |
| GACATTGATCTTCGTGTGGA+TGG | - | chr6.4:123888-123907 | MS.gene74448:CDS | 45.0% | |
| GACATTGTGCGTGAAATACG+CGG | - | chr6.4:123518-123537 | MS.gene74448:intron | 45.0% | |
| GCTGACATTCAAGACGAGTT+GGG | - | chr6.4:122622-122641 | MS.gene74448:CDS | 45.0% | |
| GTGAATTGTGTCTCGCCAAA+TGG | - | chr6.4:123705-123724 | MS.gene74448:CDS | 45.0% | |
| GTGGATGGCAGCTATGTTTA+TGG | - | chr6.4:123903-123922 | MS.gene74448:CDS | 45.0% | |
| TACGGTCAATGCTTATGGAC+AGG | - | chr6.4:122738-122757 | MS.gene74448:CDS | 45.0% | |
| TGCTGACATTCAAGACGAGT+TGG | - | chr6.4:122621-122640 | MS.gene74448:CDS | 45.0% | |
| !! | AATGGATGAAGGTGCTCCTA+TGG | + | chr6.4:122675-122694 | None:intergenic | 45.0% |
| CACCATTCGTGCACCTTGTT+CGG | + | chr6.4:122596-122615 | None:intergenic | 50.0% | |
| GCACATCTCTTTGCCGAACA+AGG | - | chr6.4:122580-122599 | MS.gene74448:CDS | 50.0% | |
| ! | GGCTGATGCTGTGTTGTTCT+TGG | - | chr6.4:123839-123858 | MS.gene74448:CDS | 50.0% |
| AAAGTAGCCATCGTCACCGG+AGG | - | chr6.4:122532-122551 | MS.gene74448:CDS | 55.0% | |
| CGAACAAGGTGCACGAATGG+TGG | - | chr6.4:122594-122613 | MS.gene74448:CDS | 55.0% | |
| GGCAAAGTAGCCATCGTCAC+CGG | - | chr6.4:122529-122548 | MS.gene74448:CDS | 55.0% | |
| TCAAGACACCTTCGCTACCC+AGG | + | chr6.4:123599-123618 | None:intergenic | 55.0% | |
| TGCCGAACAAGGTGCACGAA+TGG | - | chr6.4:122591-122610 | MS.gene74448:CDS | 55.0% | |
| ! | GGTGACGATGGCTACTTTGC+CGG | + | chr6.4:122530-122549 | None:intergenic | 55.0% |
| !! | CAGCGGCATTGGCAAAGAGA+CGG | - | chr6.4:122558-122577 | MS.gene74448:CDS | 55.0% |
| !! | TGGGTAGCGAAGGTGTCTTG+AGG | - | chr6.4:123598-123617 | MS.gene74448:intron | 55.0% |
| CGAGTGGCTGCATCCATTGG+CGG | - | chr6.4:122649-122668 | MS.gene74448:CDS | 60.0% | |
| CGTCTCTTTGCCAATGCCGC+TGG | + | chr6.4:122560-122579 | None:intergenic | 60.0% | |
| CTGCATCCATTGGCGGCCAT+AGG | - | chr6.4:122656-122675 | MS.gene74448:CDS | 60.0% | |
| GAGCGAGTGGCTGCATCCAT+TGG | - | chr6.4:122646-122665 | MS.gene74448:CDS | 60.0% | |
| ACCTTCGCTACCCAGGACGC+TGG | + | chr6.4:123592-123611 | None:intergenic | 65.0% | |
| GAAATACGCGGCCCGTGCGA+TGG | - | chr6.4:123530-123549 | MS.gene74448:intron | 65.0% | |
| GGTGCTCCTATGGCCGCCAA+TGG | + | chr6.4:122665-122684 | None:intergenic | 65.0% | |
| GTGTGCACAGCCAGCGTCCT+GGG | - | chr6.4:123579-123598 | MS.gene74448:intron | 65.0% | |
| TGTGTGCACAGCCAGCGTCC+TGG | - | chr6.4:123578-123597 | MS.gene74448:intron | 65.0% | |
| ! | AGACGAGTTGGGCGAGCGAG+TGG | - | chr6.4:122633-122652 | MS.gene74448:CDS | 65.0% |
| ! | ATGGTGGAGGGGCACGTGAG+GGG | - | chr6.4:123549-123568 | MS.gene74448:intron | 65.0% |
| !! | ATCGTCACCGGAGGTGCCAG+CGG | - | chr6.4:122541-122560 | MS.gene74448:CDS | 65.0% |
| !! | GATGGTGGAGGGGCACGTGA+GGG | - | chr6.4:123548-123567 | MS.gene74448:intron | 65.0% |
| ATACGCGGCCCGTGCGATGG+TGG | - | chr6.4:123533-123552 | MS.gene74448:intron | 70.0% | |
| CGTGCCCCTCCACCATCGCA+CGG | + | chr6.4:123545-123564 | None:intergenic | 70.0% | |
| GCCAATGCCGCTGGCACCTC+CGG | + | chr6.4:122551-122570 | None:intergenic | 70.0% | |
| GCCAGCGTCCTGGGTAGCGA+AGG | - | chr6.4:123588-123607 | MS.gene74448:intron | 70.0% | |
| GCTGGCACCTCCGGTGACGA+TGG | + | chr6.4:122542-122561 | None:intergenic | 70.0% | |
| GTGCCCCTCCACCATCGCAC+GGG | + | chr6.4:123544-123563 | None:intergenic | 70.0% | |
| !! | ACCGGAGGTGCCAGCGGCAT+TGG | - | chr6.4:122547-122566 | MS.gene74448:CDS | 70.0% |
| !! | CGATGGTGGAGGGGCACGTG+AGG | - | chr6.4:123547-123566 | MS.gene74448:intron | 70.0% |
| !! | CGGCCCGTGCGATGGTGGAG+GGG | - | chr6.4:123538-123557 | MS.gene74448:intron | 75.0% |
| !! | GCGGCCCGTGCGATGGTGGA+GGG | - | chr6.4:123537-123556 | MS.gene74448:intron | 75.0% |
| !! | CGCGGCCCGTGCGATGGTGG+AGG | - | chr6.4:123536-123555 | MS.gene74448:intron | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.4 | gene | 122487 | 123944 | 122487 | ID=MS.gene74448 |
| chr6.4 | mRNA | 122487 | 123944 | 122487 | ID=MS.gene74448.t1;Parent=MS.gene74448 |
| chr6.4 | exon | 123672 | 123944 | 123672 | ID=MS.gene74448.t1.exon1;Parent=MS.gene74448.t1 |
| chr6.4 | CDS | 123672 | 123944 | 123672 | ID=cds.MS.gene74448.t1;Parent=MS.gene74448.t1 |
| chr6.4 | exon | 122487 | 123023 | 122487 | ID=MS.gene74448.t1.exon2;Parent=MS.gene74448.t1 |
| chr6.4 | CDS | 122487 | 123023 | 122487 | ID=cds.MS.gene74448.t1;Parent=MS.gene74448.t1 |
| Gene Sequence |
| Protein sequence |
