AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74535

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74535.t1 MTR_2g030370 99.492 394 2 0 1 394 1 394 0.0 811
MS.gene74535.t1 MTR_2g030335 98.425 127 2 0 1 127 1 127 1.56e-88 266
MS.gene74535.t1 MTR_1g023160 32.117 137 74 3 27 163 272 389 1.29e-12 69.3
MS.gene74535.t1 MTR_2g019790 30.986 142 77 3 27 167 321 442 3.14e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74535.t1 AT1G30580 85.279 394 58 0 1 394 1 394 0.0 711
MS.gene74535.t1 AT1G56050 41.053 380 218 5 14 388 43 421 1.93e-94 289
MS.gene74535.t1 AT1G07615 33.000 100 50 1 27 126 306 388 2.56e-11 65.5
MS.gene74535.t1 AT5G18570 33.000 100 51 1 27 126 381 464 8.87e-11 64.3

Find 104 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAATCCAGTCTTTATTATTT+TGG 0.087619 2.3:+59474819 None:intergenic
GAAACTTGTTCTTCTTTCTT+TGG 0.236801 2.3:+59476391 None:intergenic
TACGTACCATGTGTGAATTT+TGG 0.247433 2.3:+59476365 None:intergenic
AGTGTAGACTTTCCAACATT+TGG 0.320168 2.3:+59479816 None:intergenic
AACTGGAGCTTCCTTCGATT+TGG 0.332167 2.3:+59480853 None:intergenic
TTCTCTTCTCACCTCAAGAT+TGG 0.333366 2.3:-59480809 MS.gene74535:CDS
TTTGTCCACCACCATGTTCC+TGG 0.334504 2.3:+59476007 None:intergenic
TGACCCTGTGAGGGATTTAG+AGG 0.338036 2.3:-59479234 MS.gene74535:CDS
CAGCTTGAGGAGCCTTTGTT+AGG 0.339703 2.3:+59474113 None:intergenic
ATCCTTTCTCAAAATCAGTA+TGG 0.361106 2.3:+59474080 None:intergenic
CCAAGGATGGGTCGTTCAAC+TGG 0.369239 2.3:+59480836 None:intergenic
TCCTTCGATTTGGCGGATTT+AGG 0.369949 2.3:+59480863 None:intergenic
AGCCACTCAAAACGCTCGTC+AGG 0.375759 2.3:+59479708 None:intergenic
TCTCACATCCGGGCTGTTGA+TGG 0.377798 2.3:-59479424 MS.gene74535:CDS
GCTCATGAGGGGCAAGGGTT+GGG 0.388174 2.3:-59479460 MS.gene74535:CDS
TCGTCTACGTGGATAATATC+TGG 0.394590 2.3:+59479263 None:intergenic
TGCCAAGCCTGTTGTATACT+TGG 0.397853 2.3:-59477537 MS.gene74535:intron
ATTGTTTATTATTAGGCTGC+TGG 0.416370 2.3:-59473182 MS.gene74535:intron
CAGTATTTAGCCGCCTCATC+AGG 0.422088 2.3:+59475937 None:intergenic
CGAGCTTGAATGTTGTCAAA+AGG 0.422523 2.3:-59478657 MS.gene74535:intron
ATTAGGCTCAATAGTGCAAA+AGG 0.425631 2.3:+59479749 None:intergenic
ACATTGACCCTTGCCTCATT+AGG 0.436232 2.3:+59479732 None:intergenic
AAAATATAATGTCACCATCT+TGG 0.441622 2.3:+59473126 None:intergenic
ATGTGGATATTTACCTCATC+AGG 0.442858 2.3:+59474760 None:intergenic
CAGGCTTGGCAGTAAGCAAC+TGG 0.446559 2.3:+59477549 None:intergenic
AAGTTCAATGTTTCTGGTGG+AGG 0.450367 2.3:-59473101 MS.gene74535:CDS
TGGAGCTTCCTTCGATTTGG+CGG 0.453358 2.3:+59480856 None:intergenic
TTGTTTGGTAGGTGCATTTG+AGG 0.455060 2.3:-59479288 MS.gene74535:intron
TGCTCATGAGGGGCAAGGGT+TGG 0.460273 2.3:-59479461 MS.gene74535:CDS
AAGATGTACGCCTAGGGGAT+TGG 0.461198 2.3:-59477604 MS.gene74535:CDS
TATGTTACCAAGTATACAAC+AGG 0.463776 2.3:+59477530 None:intergenic
TTAGAAATCCATGACATAGC+TGG 0.466153 2.3:-59479496 MS.gene74535:CDS
TTTCAATACACTCACAAAGA+TGG 0.470261 2.3:-59479793 MS.gene74535:CDS
AGGTTATTACTGAAGAGTTA+CGG 0.474516 2.3:-59479214 MS.gene74535:CDS
ACATTTGTTCTCCAGGGTCC+AGG 0.476574 2.3:-59476025 MS.gene74535:intron
AATCCATGACATAGCTGGAT+TGG 0.477150 2.3:-59479491 MS.gene74535:CDS
TTCTCCAGGGTCCAGGAACA+TGG 0.481367 2.3:-59476018 MS.gene74535:intron
CGAGGTGCTCATGAGGGGCA+AGG 0.481862 2.3:-59479466 MS.gene74535:CDS
TGGAAGTGAGTCAGCTGTAA+AGG 0.482331 2.3:-59473422 MS.gene74535:intron
CTTCCCAAAATAATAAAGAC+TGG 0.484849 2.3:-59474823 MS.gene74535:CDS
CCAGTTGAACGACCCATCCT+TGG 0.486370 2.3:-59480836 MS.gene74535:CDS
GATGAAATTTGATGATCTAA+AGG 0.491073 2.3:-59473449 MS.gene74535:CDS
TTTGATGATCTAAAGGAACT+TGG 0.496739 2.3:-59473442 MS.gene74535:CDS
TTGCACTATTGAGCCTAATG+AGG 0.504731 2.3:-59479745 MS.gene74535:CDS
ACAAAGGCTCCTCAAGCTGC+AGG 0.508694 2.3:-59474109 MS.gene74535:CDS
CAGGTTGGTTTGCCAAATGT+TGG 0.517974 2.3:-59479828 MS.gene74535:CDS
AGAAGAGAAACGACCAAGGA+TGG 0.519305 2.3:+59480823 None:intergenic
TCCTAAATCCGCCAAATCGA+AGG 0.520199 2.3:-59480864 MS.gene74535:CDS
TACTGAAGAGTTACGGCTGA+AGG 0.521340 2.3:-59479207 MS.gene74535:intron
GCTGGAAAATATAAACAAGA+AGG 0.524187 2.3:-59473164 MS.gene74535:CDS
TTAGGCTCAATAGTGCAAAA+GGG 0.530041 2.3:+59479750 None:intergenic
TTCAAGTTCAATGTTTCTGG+TGG 0.536287 2.3:-59473104 MS.gene74535:CDS
CAAAAGGGGAAGTTCTCAGC+AGG 0.538630 2.3:+59479765 None:intergenic
GACATAGCTGGATTGGTTCG+AGG 0.547428 2.3:-59479484 MS.gene74535:CDS
TTGGTTCGAGGTGCTCATGA+GGG 0.547681 2.3:-59479472 MS.gene74535:CDS
ATAACCTCTAAATCCCTCAC+AGG 0.552003 2.3:+59479230 None:intergenic
GCTTCTTTCAGGTTAAGTGT+TGG 0.554283 2.3:-59474147 MS.gene74535:intron
AGGGAAAAGATGTACGCCTA+GGG 0.554324 2.3:-59477610 MS.gene74535:CDS
TTCGATTTGGCGGATTTAGG+AGG 0.558249 2.3:+59480866 None:intergenic
CGGCAGCTTTCCAATCCCCT+AGG 0.561018 2.3:+59477594 None:intergenic
AGTTCAATGTTTCTGGTGGA+GGG 0.562003 2.3:-59473100 MS.gene74535:CDS
TTCAGATATGCCTCCTGATG+AGG 0.565036 2.3:-59475950 MS.gene74535:CDS
GAAAGGATTTATTTGTGCTG+AGG 0.566045 2.3:-59474065 MS.gene74535:intron
ATTGGTTCGAGGTGCTCATG+AGG 0.566137 2.3:-59479473 MS.gene74535:CDS
AAAACTTATGTGGTCCAAGA+TGG 0.567578 2.3:-59473140 MS.gene74535:CDS
TGGATAGCCCCTGCAGCTTG+AGG 0.571503 2.3:+59474100 None:intergenic
GAGGGAAAAGATGTACGCCT+AGG 0.572957 2.3:-59477611 MS.gene74535:CDS
CAAAGGCTCCTCAAGCTGCA+GGG 0.574970 2.3:-59474108 MS.gene74535:CDS
TCAGGTCAAGGCATTCATTG+AGG 0.575043 2.3:-59477633 MS.gene74535:intron
GAACCAATCCAGCTATGTCA+TGG 0.579932 2.3:+59479488 None:intergenic
CTATTGAGCCTAATGAGGCA+AGG 0.581575 2.3:-59479740 MS.gene74535:CDS
CTGGAAAATATAAACAAGAA+GGG 0.582742 2.3:-59473163 MS.gene74535:CDS
GTGAGGCGAACAACGTTCAA+AGG 0.582805 2.3:-59475915 MS.gene74535:intron
TACCAAGTATACAACAGGCT+TGG 0.583923 2.3:+59477535 None:intergenic
AGGGTCCAGGAACATGGTGG+TGG 0.584742 2.3:-59476012 MS.gene74535:CDS
ATCTTGAAAAGAGCATGAAG+AGG 0.584769 2.3:-59478703 MS.gene74535:CDS
TTCTCTAAGACACAGCTGAA+AGG 0.586644 2.3:+59475979 None:intergenic
GAGGTGCTCATGAGGGGCAA+GGG 0.588995 2.3:-59479465 MS.gene74535:CDS
GTCAAGGCATTCATTGAGGA+GGG 0.593032 2.3:-59477629 MS.gene74535:CDS
AGAATACGATTCCAATCTTG+AGG 0.593260 2.3:+59480798 None:intergenic
ACAAGAAGGGAAAACTTATG+TGG 0.593833 2.3:-59473150 MS.gene74535:CDS
AAAGGCTCCTCAAGCTGCAG+GGG 0.596118 2.3:-59474107 MS.gene74535:CDS
GCAAATTAGACGCCTAACAA+AGG 0.601410 2.3:-59474125 MS.gene74535:CDS
GGTCAAGGCATTCATTGAGG+AGG 0.601452 2.3:-59477630 MS.gene74535:CDS
GCCACTCAAAACGCTCGTCA+GGG 0.607437 2.3:+59479709 None:intergenic
GAAGAGAAACGACCAAGGAT+GGG 0.608124 2.3:+59480824 None:intergenic
AGGGGATTGGAAAGCTGCCG+AGG 0.611014 2.3:-59477591 MS.gene74535:CDS
TAGGCTCAATAGTGCAAAAG+GGG 0.614822 2.3:+59479751 None:intergenic
TTTCACAGCAGGACCTGATG+AGG 0.615456 2.3:-59474773 MS.gene74535:intron
TGGTTCGAGGTGCTCATGAG+GGG 0.617191 2.3:-59479471 MS.gene74535:CDS
TATTGAGCCTAATGAGGCAA+GGG 0.617548 2.3:-59479739 MS.gene74535:CDS
TATTTAGCCGCCTCATCAGG+AGG 0.619128 2.3:+59475940 None:intergenic
TTCTTCCCAAAATTCACACA+TGG 0.624147 2.3:-59476371 MS.gene74535:intron
GGAATTCAAAATCTCGACCT+CGG 0.625649 2.3:+59477574 None:intergenic
CTTGGCAGTAAGCAACTGGA+AGG 0.630484 2.3:+59477553 None:intergenic
CGACACTGTTGACCCTGTGA+GGG 0.636940 2.3:-59479243 MS.gene74535:CDS
AGATATGCCTCCTGATGAGG+CGG 0.638742 2.3:-59475947 MS.gene74535:CDS
ACGACACTGTTGACCCTGTG+AGG 0.640959 2.3:-59479244 MS.gene74535:CDS
TCCAGGGTCCAGGAACATGG+TGG 0.661205 2.3:-59476015 MS.gene74535:intron
GGTCAACAGTGTCGTCTACG+TGG 0.682008 2.3:+59479252 None:intergenic
GGGAAAAGATGTACGCCTAG+GGG 0.687762 2.3:-59477609 MS.gene74535:CDS
GGTGAGAAGAGAAACGACCA+AGG 0.698806 2.3:+59480819 None:intergenic
TAACCTCTAAATCCCTCACA+GGG 0.708122 2.3:+59479231 None:intergenic
TGAGGCGGCTAAATACTGTG+AGG 0.765226 2.3:-59475932 MS.gene74535:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr2.3 gene 59473091 59480891 59473091 ID=MS.gene74535
chr2.3 mRNA 59473091 59480891 59473091 ID=MS.gene74535.t1;Parent=MS.gene74535
chr2.3 exon 59480805 59480891 59480805 ID=MS.gene74535.t1.exon1;Parent=MS.gene74535.t1
chr2.3 CDS 59480805 59480891 59480805 ID=cds.MS.gene74535.t1;Parent=MS.gene74535.t1
chr2.3 exon 59479680 59479847 59479680 ID=MS.gene74535.t1.exon2;Parent=MS.gene74535.t1
chr2.3 CDS 59479680 59479847 59479680 ID=cds.MS.gene74535.t1;Parent=MS.gene74535.t1
chr2.3 exon 59479407 59479530 59479407 ID=MS.gene74535.t1.exon3;Parent=MS.gene74535.t1
chr2.3 CDS 59479407 59479530 59479407 ID=cds.MS.gene74535.t1;Parent=MS.gene74535.t1
chr2.3 exon 59479208 59479299 59479208 ID=MS.gene74535.t1.exon4;Parent=MS.gene74535.t1
chr2.3 CDS 59479208 59479299 59479208 ID=cds.MS.gene74535.t1;Parent=MS.gene74535.t1
chr2.3 exon 59478658 59478756 59478658 ID=MS.gene74535.t1.exon5;Parent=MS.gene74535.t1
chr2.3 CDS 59478658 59478756 59478658 ID=cds.MS.gene74535.t1;Parent=MS.gene74535.t1
chr2.3 exon 59477538 59477651 59477538 ID=MS.gene74535.t1.exon6;Parent=MS.gene74535.t1
chr2.3 CDS 59477538 59477651 59477538 ID=cds.MS.gene74535.t1;Parent=MS.gene74535.t1
chr2.3 exon 59476372 59476436 59476372 ID=MS.gene74535.t1.exon7;Parent=MS.gene74535.t1
chr2.3 CDS 59476372 59476436 59476372 ID=cds.MS.gene74535.t1;Parent=MS.gene74535.t1
chr2.3 exon 59475916 59476032 59475916 ID=MS.gene74535.t1.exon8;Parent=MS.gene74535.t1
chr2.3 CDS 59475916 59476032 59475916 ID=cds.MS.gene74535.t1;Parent=MS.gene74535.t1
chr2.3 exon 59474774 59474849 59474774 ID=MS.gene74535.t1.exon9;Parent=MS.gene74535.t1
chr2.3 CDS 59474774 59474849 59474774 ID=cds.MS.gene74535.t1;Parent=MS.gene74535.t1
chr2.3 exon 59474066 59474158 59474066 ID=MS.gene74535.t1.exon10;Parent=MS.gene74535.t1
chr2.3 CDS 59474066 59474158 59474066 ID=cds.MS.gene74535.t1;Parent=MS.gene74535.t1
chr2.3 exon 59473423 59473473 59473423 ID=MS.gene74535.t1.exon11;Parent=MS.gene74535.t1
chr2.3 CDS 59473423 59473473 59473423 ID=cds.MS.gene74535.t1;Parent=MS.gene74535.t1
chr2.3 exon 59473091 59473189 59473091 ID=MS.gene74535.t1.exon12;Parent=MS.gene74535.t1
chr2.3 CDS 59473091 59473189 59473091 ID=cds.MS.gene74535.t1;Parent=MS.gene74535.t1
Gene Sequence

>MS.gene74535

ATGCCTCCTAAATCCGCCAAATCGAAGGAAGCTCCAGTTGAACGACCCATCCTTGGTCGTTTCTCTTCTCACCTCAAGATTGGAATCGTATTCTTCTATCTCTTTCTAATCGCATCAATTTTCAATTTTTATACTATTTTCAGTAACCCTTTTTCAATTACAATAATGGGTTCTCCATTAATCAACTTCATTCCATGCTTAATTGATTCTTTATAATAGTCTAGATAGTAACTAGAACCATTGAGTTGCTGTTTATTTATTTCAATGCTTATTGATTTTGTGAAATGATTTTTTTGTTTTGAGAATGATTTGGTTTATTTGAGCTTATTTACCGACGTAATCACTTTGAGGAGCTTATGAAAATAACTTATGACAAACTGTTTGTTTGTTTGTTTCAATAGCGGATTGAGTTTGTGAAATGATAATAAAAATTGTCTGAAAACGATTATTGGCCTGTTTCTATTTGGGTTATTAAAGTTGATCAAAGGGCATAAGCAAAGAGAAGGTTTGTGAGAGCTTATGGAAACAACTTATGACATATTCAAGAGCTGTTTATCACTTTACTTCCATAAGCTCTCAAAGATAGCTTATATGAAAACGAATTTGAATTTATTTTATCTTTTGATGTAGAAATAGCTTATAGATAAGCACTTATATGAGAAACACTTGAGCTATAAGTGCTTAATTTAGCTGTTTATCCAAACAAGGCCTTTGGTTTATAGGCCTGTTTGGATATGGCTTATTTAAGTTCATCTACTTACATAATCACTATAATACTCTTTGAGAGAGCTTATGAAAACAACTTATGACAGACTATTTTGAAGAGCTTATGAAAAAGTTGTTTTCAACTTATTTTCATAATCTCTCAAGGATAGCTTATGTGAATACGATTTAACTGTATTGTATCTTTTGGTATAGAAATAGCCTATATATAAGCACTTAAATGATAAATGTTTATGCTACAAGTGCTTAATTATGTTGTTTAATCAAACATGGACTTTGTATTTTTCATTTCATGTTAGTTATTATTACTCTGTTGTTCAGGTTGGTTTGCCAAATGTTGGAAAGTCTACACTTTTCAATACACTCACAAAGATGGCAATTCCTGCTGAGAACTTCCCCTTTTGCACTATTGAGCCTAATGAGGCAAGGGTCAATGTCCCTGACGAGCGTTTTGAGTGGCTTTGTCAATTGTTCAAGCCAAAAAGCGAGGTTTGTAATCTGGCTCATACCATTGCTGGTGTATCTCTTTTGTTAATCTACTAATATACACGCATGCCGTCACTAACTTTTAGTTATCTATTATTTGATGGATAAGTGTATTATATATCCTGACGTGTTTGTTGGATCTGTAAATACAGGTCTCTGCTTTTTTAGAAATCCATGACATAGCTGGATTGGTTCGAGGTGCTCATGAGGGGCAAGGGTTGGGAAATAGTTTTTTATCTCACATCCGGGCTGTTGATGGCATTTTTCACGTTCTACGTAAGATGATTCCTGCTCCTATTTCCACACCACTTTTGCATCATGATGTTATGATATTGTCATATTGATTTCGGCGTTATTAACCTATAGTCATTGTTGTTTGGTAGGTGCATTTGAGGATCCAGATATTATCCACGTAGACGACACTGTTGACCCTGTGAGGGATTTAGAGGTTATTACTGAAGAGTTACGGCTGAAGGTATGCTTAATTGAAACCTTTTCTTTATCATAAAATTGGCTTTTGGGCCAACCATTATTTATTTTTTGAATAGGCCAATATTAGTATGTTAATTGTTATGTTAGGATATTTTTCCTCCTTGCCAACCGTTATTGTTGTTTGATCAAACATCAATTTTTTTTGATTGGTTGTAAAGTACATGGTCTTTTAAGGGGGCTTTCTTAACAAACATGTGGAAGGATGAATATCTCTGCTCCATTGATTTTGTTTTTCTTTAATATCTGTCTGTTTATCTATGTGTGTTTTAACGCTAAGGAGGATCCCTTTATTCCAAATTCATTGTAATTTCTTTTTTTTTTTAGTTTTGATGAATTTATTATTTTATCAAAATTTAAAGGTTGTACCTTTTCAATTGAACTTCTTGTTTACTGTAACGTAACTGCTAATCCTTTCCCTTTGCGTAACATCATAGGATATTGAATTCATGCAAAACAAGTTAGAAGATCTTGAAAAGAGCATGAAGAGGAGCAATGACAAGCAGTTAAAAATCGAGCTTGAATGTTGTCAAAAGGTTTGATTTAGCTTCTGAGCATTATTTGTACTCATTTAGCTTTTGCATGATACAGTAGAAACTCTTTAATTTAATACTCGGTAAATTAATAAACTCTCTAAAATAATAAATTTGTCCGGTCCCGACTTGGGTCAATGTAAAAAATTGAAAAACTCGCTAAAATAATAAGATAATAATTTTTTGGGAGATCCCTTTATAATTTTCGGTCCCAAGAAAATCATAAATTAATAATTCATAGAAACTGAAAAAATATATATTAAGAATACATTGAACACATTTGTTCCTCTTGTTTACATTTACTGTACTTCAAAAGTCATTATTGATTTCCAATGCATATGAACACAGTAGTTACTAATTTACGTTGAGTCCCAAGGTTCGCTGCAACGTAATTCTAAGAGGCATAAACTAATTTGCCTTTTTGTTTGGTATGTACAGTATAATTACTTAAAAACTCTTTAAATTAATAATTATTAATTTATCGATAAGTTAATAACTCTCTAAATTAATAAATTTCTCCGGTCCTGACATTATTAATTTATAGAGTTTTAACTGTACTATGGCCATTGGCCACCTTTTGTCTTGTTATTTTATATAATTTACAGTTTACTACTATTGTATCTGTTATGTGTTGTCTTACTTTTTTTTTTTGGGTGTTTTATTGGTGTTTTCTTCGTATGATTCTCTCTGGATTCCACAATACATTACTTTAATAAAATGGGAGTAGTTGCTTGCAACAATATTCTCAAATTTGGGTTGAAATATAATGTAAATAATCAAATCAATTGCCTCACTGGATGACTTATATTAGCTCTTAAAGAAGCATGGAAATTAAGTATGTGTTCATTTTTATGTTATGCATTGCATTCTCTCATAAGGTTGATTCTATAAATGTAATGTCACATTCCTTTTCTCTTCTCATGTGTTTAAGTTTTGTAAATATACACTCAAAAAAAGTTGTGGGTAAATATAAGATGAACATTTTGGTGGCAATTTTTTACATCTTCAGGTCAAGGCATTCATTGAGGAGGGAAAAGATGTACGCCTAGGGGATTGGAAAGCTGCCGAGGTCGAGATTTTGAATTCCTTCCAGTTGCTTACTGCCAAGCCTGTTGTATACTTGGTAACATATTTCACTTCTCTTTCTTGCTTTTGACTTTTTGTATGTCTTTGGCTTACATACAACCTGTAATCTATGTCATTTCGTATCATATTCTACTACCAATTATTTCGCGTTACCAACGATACTTAACAGTGCTACATAATTTGGGATATGTCTTGATGGATATGTGTCAGAAGTGTCTGTTTTCTATATGTTTGGAAAATATGAAATACTAATTTGATATTTCTCAAAGTTGGCTGGATATGGCTCGGGTATATTGATGATGTGATTATTAGCTGCGATTGACTCCACATTGTAATTTCTTTTATAGGAAACTCCTAAGTATAATAATCGTTTGGTTGAAATGATAACATTAGAGAGAGAGAGAGAGAGAGAGAGTTGGGGTAGCACCTATAGTAGAAAATATGGTGGAAAATAGGCTTAGGTGGTTTGGGCATGTAGAGAGAAGACCTGTAGATTCTGTAGTAGAGAGTAGATCAGATGGAGGGGTAGTCAGATCACTAGAGCCAGAGGAAAACCTAGAAAAACTATAAGTGAAACCATTAGGAACGATTAGAGATTAATGAGTTGGATCCAAATATGGTTTATGATTAATAGAACATTATGGCGTAATTTGATCCATGTAGCCGAACTCACTTAGTGGGATAAGGCTTGGTTGTTGTTGTGGTTGAAATTCAAATATGAACATAAGTTTGATTATTTTCTATCCAAAGGCATTTGCAACTGCACAAGTTGACAAAACGATGTATGATCTGGTATTCATTTCCTCTTTCCCAGCCTGCGATATGAACCATTCATTCGCATACCTTTTTCTTGTGAAGGCCACATTTCATACATACTTCCATCCAAACACGTCGGCATATATAAGTTCATTTTATAATTATTTAATTTTGTTATCTTTGCCAATTGCCATTTCATGTTCTAGAATTTTCAGAACTCGCCTTATCACTCTATGTATTTGTGTCCGTCACATATGTTTAGTGTGAAGTATATATAATTAGTGACTGATAAATTTAATCAAAATCCATATTGTTTCATGTACTTTATCTCAGATCTGTTTATGTGAATTTTCCATCTTTAATACTTGCATTTTATTCACAGATTAATATGACTGAAAGAGATTACCAAAGAAAGAAGAACAAGTTTCTTCCCAAAATTCACACATGGTACGTAGTTATTTTTTTTCTTTCCATGTCATTTTTTTCTTCTGGGAGCCATTCAGACTTTGTCTCATTCCTGGCTACCTCTGTGCGAATATGAAGGATGCAATGAATAGTTGCAAAGTATTTTAGTTATTTCTTTCTACGAGCCACTAGAAGAAGAAAATATTTACTAGTTTGTCACATTTTGTTGCTTCTTGTGTATTACTGTTTACTTTTAACTTATATTTCTTATTGTGCCTAGTTTTTCTGTTTTTCCTTATTATTCTCTAAATATTTGTTGGTTTATGATGTATTAATGTCAAAAAAGAAATTTAATTACCATTAGTCACATTTGTTCTCCAGGGTCCAGGAACATGGTGGTGGACAAATGATTCCTTTCAGCTGTGTCTTAGAGAAGAATCTTTCAGATATGCCTCCTGATGAGGCGGCTAAATACTGTGAGGCGAACAACGTTCAAAGGTCTGTACAATGTATTTGCCTTACTTCTATTAAATGTATGAATGTGAAGTTATTATGCAAATATTATATTTGAGAAGACTGAATTTACTTATACATCAAATATTAAGGTCTTGAGTATATGTTGAAATATTCATTTTCATTCTTTTATACCTTAAAAAAATTTCAACTTAATAGACACCACATAGATTCATATTTTATAGATATCCGGCCTTGACCAGTCAGTCAGACGGATCTAACAAGGAACCTAAATTGGGTTAGTCCTGTCAGGTACCGGTCGAAAAACTGCGAACCAGTGAAACTCTGTTATTCTCACAATGAACCAGTTTCTTTGCTTAAAAAAATAGTATTTTTTTAATCGTATGAATCAGACACATTGGCTTATACAGTCTTACCAGTTACCATTAATATGCAACTACTATTCTTTATTATTTCCAAAATGTATCATACTATACTGCACGCGCACGCGCGCACGCGCACACACACACACACACACACATATATATATTGAACAAGTATTTATTTATTTCTGGTTCGACCATGGTGTGATTATAGTCAGACCATTGAACTGTGAAGCAATATTTTCATCGCTGAATGATTGGTTCAGTTTAAAATACACCGGGTGTCTGAGCTAAGTAGCTGACCCCACTTAGTGGGATAAGGCTTGGTTGTTGTGGGTGTCTGAGCTAACTTACCGCTTTCACATGTCATATATTATCCCAAATTCATTTTCCCTTGGTCGCCATTGCCATCCAAATTTGAAAGTCGCAGACGTGTAAAATATCAGTAAAACCTAATATTCATTATTTTCTGTGCATATGCACTATGTGTCTATGCTTTTGAAGTGAGAATTAGCCTTGTTCTCTTTTAAACAAATTGAGAGAAAAAATCCCTTTTTATCACAATTAAGATTACTACATCAGACATTAGAAGCTTATCCTGTTTGCCTTTGTTTTTTATTTTTTAATCTCCTTTTTCATAAGAAGTTCTAGCTGAATCCATATTAATTTTAAAGATTTGGTGCATAATTTATATGCTACTGAAATTGCTATATCTTTCATTTTAACAGTGCTCTTCCCAAAATAATAAAGACTGGATTCTCAGCTATCAATCTCATTTACTTTTTCACAGCAGGACCTGATGAGGTAAATATCCACATATCTCCTTTCACACTGTTAGTTGTCTTCTAAATAGTTATGAGTGTTCACGGTCTTGGTTTCATAACCTTGCCAAACTATAATTATTAGTTTCTCCCATAGTTGGCAAAATCTTACGATTTATAATTTGAAATGCACGGTTCTATACTTGGTAATGGGACAACTATTTTAGTCATATGATGAATAACAAATGAATTTAAATCGTACAATCAATGAGTTGAATTTGAATCATGCAATTGATGAATGAATCATCAGTGTGATTTGTATCATTGATAGGAATAGTTCGATTCAAATACTTGTTTTTTAGTTTACTTTTTCATGTGCTTCTTGTCACATATATGGTAAAGTCAAATGCAATTATATTATTGAATGAAAAGAAACTGAAGGGCTGGTATGAAATGCATGTGTTTGTTAACACCTCCCTGCACCCCCTTCCTTTGTTCTGATGTGTTCCTTTGTCGAGGCTTCATGGTGGTTCTTTCTCATTCTGATTTGAGAACTGTTATTTGCTTGAATTCTTTTGTTTGGTTTTTCCATGTCTAAGGTACAGCTTTTGGTGCCTTTCATTTCGTAACTTTTGTGGCTATTATTTGCTTCTTTCAGGTTAAGTGTTGGCAAATTAGACGCCTAACAAAGGCTCCTCAAGCTGCAGGGGCTATCCATACTGATTTTGAGAAAGGATTTATTTGTGCTGAGGTAAGTTCATTAATTTTGGACACTTCTTGATCAACCATATTATCTCTCCTATTTAACTTATTTACGTAGCATTGTGACATCTATACATATCTATTCAGCAATTAAATTATCTGCAAATGCTTTATTGCACCAGCATTTATTTTTATTTTTTACAAACAAGAACAGGAATTCTCAGATTCTAAGATTGAATACTAAAGGCATCCTTTCCTTCACGACAAATTTAGAAATGAATTCTTCTTTATGATTCTAATCAAGCTGTTTCACCCTTGCAATATGAATTACCGTCGGATAAGAATCTAATTGAAGTATGCTTTTCTTAAGATTGTTTCTTATTCTATGGCACAATCACTGTTACTCATTGTGGTTTGGTCTACAATCATCCAACAGTTCTTATTCTATTTTCTTGGCGGCCTTTATGGCATAGCCATTCCCCTTATGAAAATATGTGTTCACTGGTTATGTAGTTTAAATTCTCATGATTGAATTTGATCTGTTAATTTTTTTTATTTTTTTATTTTTTATGTTTTCGCTGATACAGCGACTTCTATATATATACACCACTGATCTAGGTGCTTTGCAAATGTCGTTTCAGGTGATGAAATTTGATGATCTAAAGGAACTTGGAAGTGAGTCAGCTGTAAAGGTATGCACTTCTATTCTTTAGATATAACTTTTTTTGTATTTCTATTGATTCCTAAAATGTTTAGATATAACTGTAATGGAACAAAAATTTGAGACAACACTTCAGGTCTGACTTCTTAGCTTAATTGTGGCAAATATTTGAAACATTTGTGTAAATTTGTTTATGTTGTTAGTTTATAGGTTCTTTTAGGGACCTAACCATATCTCACTCTTTGTTGTATTGTTTATTATTAGGCTGCTGGAAAATATAAACAAGAAGGGAAAACTTATGTGGTCCAAGATGGTGACATTATATTTTTCAAGTTCAATGTTTCTGGTGGAGGGAAGAAGTGA
Protein sequence

>MS.gene74535.t1

MPPKSAKSKEAPVERPILGRFSSHLKIGIVGLPNVGKSTLFNTLTKMAIPAENFPFCTIEPNEARVNVPDERFEWLCQLFKPKSEVSAFLEIHDIAGLVRGAHEGQGLGNSFLSHIRAVDGIFHVLRAFEDPDIIHVDDTVDPVRDLEVITEELRLKDIEFMQNKLEDLEKSMKRSNDKQLKIELECCQKVKAFIEEGKDVRLGDWKAAEVEILNSFQLLTAKPVVYLINMTERDYQRKKNKFLPKIHTWVQEHGGGQMIPFSCVLEKNLSDMPPDEAAKYCEANNVQSALPKIIKTGFSAINLIYFFTAGPDEVKCWQIRRLTKAPQAAGAIHTDFEKGFICAEVMKFDDLKELGSESAVKAAGKYKQEGKTYVVQDGDIIFFKFNVSGGGKK