AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene74967


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74967.t1 MTR_1g112200 98.095 210 4 0 1 210 1 210 3.64e-151 418
MS.gene74967.t1 MTR_1g060120 56.373 204 78 1 17 209 20 223 7.58e-82 243
MS.gene74967.t1 MTR_1g085500 42.381 210 114 3 7 209 7 216 3.68e-53 170
MS.gene74967.t1 MTR_7g111240 45.596 193 99 4 22 209 19 210 7.77e-53 169
MS.gene74967.t1 MTR_7g111240 44.898 196 99 4 22 209 19 213 3.13e-52 167
MS.gene74967.t1 MTR_1g085140 43.434 198 105 3 19 209 20 217 2.28e-51 165
MS.gene74967.t1 MTR_6g005330 43.721 215 105 6 7 207 3 215 1.45e-47 155
MS.gene74967.t1 MTR_6g005310 43.981 216 106 6 5 207 2 215 2.75e-47 155
MS.gene74967.t1 MTR_1g079490 44.059 202 98 6 19 207 18 217 4.85e-47 154
MS.gene74967.t1 MTR_6g005360 43.721 215 108 5 5 207 2 215 5.27e-47 154
MS.gene74967.t1 MTR_6g005340 44.700 217 103 8 5 207 2 215 6.00e-47 154
MS.gene74967.t1 MTR_5g046410 41.627 209 90 7 19 206 18 215 2.00e-43 145
MS.gene74967.t1 MTR_5g046430 41.627 209 90 7 19 206 18 215 2.00e-43 145
MS.gene74967.t1 MTR_4g017030 41.627 209 90 7 19 206 18 215 2.00e-43 145
MS.gene74967.t1 MTR_4g017050 41.546 207 93 7 19 206 18 215 2.02e-43 145
MS.gene74967.t1 MTR_4g017040 40.179 224 97 8 9 206 3 215 2.86e-43 145
MS.gene74967.t1 MTR_2g019250 41.627 209 90 7 19 206 18 215 5.06e-43 144
MS.gene74967.t1 MTR_1g023770 39.109 202 116 4 11 207 10 209 9.98e-43 143
MS.gene74967.t1 MTR_5g036570 41.753 194 105 4 22 207 26 219 1.07e-42 143
MS.gene74967.t1 MTR_2g030855 40.513 195 108 5 20 207 19 212 4.43e-42 141
MS.gene74967.t1 MTR_2g031270 40.957 188 103 5 27 207 27 213 9.29e-42 140
MS.gene74967.t1 MTR_6g005350 40.196 204 100 6 19 207 18 214 8.94e-38 130
MS.gene74967.t1 MTR_2g041580 36.946 203 119 3 9 204 3 203 2.70e-37 129
MS.gene74967.t1 MTR_2g041570 36.765 204 120 3 9 205 3 204 2.79e-37 129
MS.gene74967.t1 MTR_2g030895 41.860 172 94 4 42 207 7 178 5.85e-37 127
MS.gene74967.t1 MTR_2g041550 36.816 201 122 2 9 204 3 203 8.18e-37 128
MS.gene74967.t1 MTR_8g020590 36.058 208 115 5 10 205 1 202 1.14e-35 125
MS.gene74967.t1 MTR_2g030865 41.279 172 95 4 42 207 7 178 1.37e-35 124
MS.gene74967.t1 MTR_3g019710 34.300 207 125 5 8 207 3 205 1.05e-34 122
MS.gene74967.t1 MTR_8g020630 35.437 206 124 6 8 207 3 205 3.91e-34 121
MS.gene74967.t1 MTR_2g086640 34.286 210 112 5 12 208 8 204 1.78e-33 119
MS.gene74967.t1 MTR_8g020610 32.367 207 129 5 8 207 1 203 5.26e-33 118
MS.gene74967.t1 MTR_2g036120 33.333 207 127 5 8 207 3 205 6.87e-33 117
MS.gene74967.t1 MTR_6g005380 39.109 202 108 5 7 197 9 206 7.52e-33 117
MS.gene74967.t1 MTR_2g045100 36.517 178 106 4 33 207 32 205 7.81e-33 117
MS.gene74967.t1 MTR_3g019680 35.266 207 119 6 8 207 6 204 1.32e-32 117
MS.gene74967.t1 MTR_8g020650 37.433 187 108 6 27 207 22 205 1.44e-32 117
MS.gene74967.t1 MTR_3g019650 35.749 207 118 7 8 207 6 204 5.14e-32 115
MS.gene74967.t1 MTR_8g007300 35.294 187 113 2 22 200 25 211 9.90e-32 115
MS.gene74967.t1 MTR_3g096330 41.765 170 87 2 15 172 563 732 1.24e-31 121
MS.gene74967.t1 MTR_2g044040 32.850 207 128 5 8 207 3 205 1.41e-31 114
MS.gene74967.t1 MTR_8g020670 33.990 203 125 5 11 207 6 205 2.96e-31 113
MS.gene74967.t1 MTR_4g010340 33.163 196 122 4 16 207 11 201 3.65e-31 113
MS.gene74967.t1 MTR_8g020763 34.706 170 107 3 40 207 19 186 2.59e-30 110
MS.gene74967.t1 MTR_4g052770 33.019 212 122 5 5 208 7 206 3.06e-30 110
MS.gene74967.t1 MTR_8g020750 33.173 208 128 4 8 207 4 208 5.98e-30 110
MS.gene74967.t1 MTR_0284s0010 32.850 207 128 5 8 207 3 205 8.07e-30 110
MS.gene74967.t1 MTR_2g086620 31.731 208 120 5 12 208 9 205 9.37e-30 109
MS.gene74967.t1 MTR_2g086630 31.731 208 120 5 12 208 9 205 9.68e-30 109
MS.gene74967.t1 MTR_4g052780 37.500 160 96 2 53 208 47 206 2.83e-29 108
MS.gene74967.t1 MTR_2g031300 40.625 160 70 4 42 196 7 146 6.86e-28 103
MS.gene74967.t1 MTR_3g073170 34.595 185 110 5 29 207 27 206 1.14e-27 104
MS.gene74967.t1 MTR_7g011340 31.351 185 122 2 27 207 22 205 3.56e-27 103
MS.gene74967.t1 MTR_5g031460 30.435 207 133 5 8 207 3 205 7.67e-27 102
MS.gene74967.t1 MTR_8g020770 30.097 206 137 3 8 207 4 208 1.05e-26 102
MS.gene74967.t1 MTR_8g020760 32.857 210 125 8 8 207 4 207 1.09e-26 102
MS.gene74967.t1 MTR_2g019780 32.258 186 113 7 29 207 29 208 2.50e-24 95.9
MS.gene74967.t1 MTR_3g019810 28.846 208 133 5 3 204 104 302 3.43e-21 89.4
MS.gene74967.t1 MTR_4g027415 35.200 125 70 5 27 144 22 142 1.17e-13 65.9
MS.gene74967.t1 MTR_2g072560 32.759 116 68 4 8 116 3 115 3.12e-11 59.3
MS.gene74967.t1 MTR_3g019610 34.906 106 59 4 8 106 3 105 4.10e-11 58.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene74967.t1 AT3G10080 60.194 206 70 3 13 209 19 221 1.50e-86 255
MS.gene74967.t1 AT3G10080 60.194 206 70 3 13 209 90 292 5.86e-86 256
MS.gene74967.t1 AT1G02335 42.326 215 116 4 1 207 1 215 7.29e-56 177
MS.gene74967.t1 AT3G04200 45.070 213 104 5 8 209 14 224 1.08e-53 172
MS.gene74967.t1 AT1G18980 45.192 208 105 4 7 207 13 218 9.06e-53 169
MS.gene74967.t1 AT1G18970 42.308 208 111 4 7 207 13 218 1.05e-51 166
MS.gene74967.t1 AT5G26700 40.865 208 116 4 7 209 6 211 1.91e-51 165
MS.gene74967.t1 AT3G62020 43.204 206 109 4 12 209 12 217 2.80e-51 165
MS.gene74967.t1 AT5G39160 41.346 208 112 5 7 207 11 215 1.23e-50 163
MS.gene74967.t1 AT5G39160 40.845 213 110 6 7 207 11 219 1.43e-50 163
MS.gene74967.t1 AT5G39130 40.376 213 111 6 7 207 11 219 2.33e-50 163
MS.gene74967.t1 AT5G39190 40.845 213 110 6 7 207 11 219 2.49e-50 163
MS.gene74967.t1 AT1G09560 44.103 195 102 3 22 209 23 217 3.41e-50 162
MS.gene74967.t1 AT5G38960 44.059 202 99 5 19 207 22 222 5.02e-50 162
MS.gene74967.t1 AT3G05950 43.925 214 105 6 7 208 12 222 8.93e-50 162
MS.gene74967.t1 AT3G05930 38.710 217 125 3 1 209 1 217 8.53e-49 159
MS.gene74967.t1 AT5G39110 41.014 217 113 6 5 207 5 220 2.39e-48 158
MS.gene74967.t1 AT5G39180 40.845 213 112 5 8 207 8 219 9.00e-48 156
MS.gene74967.t1 AT5G38910 42.326 215 109 6 6 207 7 219 1.02e-47 156
MS.gene74967.t1 AT3G62020 45.699 186 95 4 28 209 5 188 1.43e-47 155
MS.gene74967.t1 AT5G38940 42.424 198 101 5 22 207 25 221 1.50e-47 155
MS.gene74967.t1 AT5G38940 42.424 198 101 5 22 207 49 245 2.10e-47 156
MS.gene74967.t1 AT5G39150 40.845 213 112 5 8 207 8 219 2.26e-47 155
MS.gene74967.t1 AT5G39120 40.639 219 113 6 5 207 2 219 5.68e-47 154
MS.gene74967.t1 AT5G39160 39.130 207 100 6 7 207 11 197 4.27e-45 149
MS.gene74967.t1 AT5G39190 39.130 207 100 6 7 207 11 197 6.31e-45 148
MS.gene74967.t1 AT3G04180 39.500 200 110 4 20 208 23 222 8.87e-45 149
MS.gene74967.t1 AT4G14630 39.500 200 107 5 21 207 24 222 1.89e-44 148
MS.gene74967.t1 AT3G04170 41.315 213 111 6 8 210 13 221 2.35e-44 148
MS.gene74967.t1 AT3G04150 39.623 212 115 5 8 208 13 222 6.02e-44 147
MS.gene74967.t1 AT5G38930 40.201 199 106 5 21 207 24 221 2.12e-43 145
MS.gene74967.t1 AT3G04190 40.299 201 107 5 20 208 23 222 2.70e-43 145
MS.gene74967.t1 AT3G04150 38.222 225 113 6 8 208 13 235 2.55e-42 143
MS.gene74967.t1 AT5G20630 38.798 183 107 2 27 204 22 204 1.57e-39 135
MS.gene74967.t1 AT1G10460 36.181 199 113 5 22 207 18 215 6.47e-39 133
MS.gene74967.t1 AT5G61750 38.983 177 101 3 9 178 5 181 1.98e-34 122
MS.gene74967.t1 AT1G74820 31.390 223 134 6 2 208 7 226 3.77e-33 119
MS.gene74967.t1 AT1G72610 36.508 189 103 3 27 204 19 201 4.23e-33 118
MS.gene74967.t1 AT5G38910 35.681 213 89 6 6 207 7 182 5.36e-31 112
MS.gene74967.t1 AT5G38950 49.206 63 30 1 138 198 2 64 2.99e-12 61.2

Find 21 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGATTAAAGATGAGCTTT+TGG 0.263245 1.3:+76070707 MS.gene74967:CDS
GAATTTGTTATCTTCTGATT+TGG 0.267937 1.3:-76070259 None:intergenic
CCAGTATCTTTGAAGTTTCC+TGG 0.322734 1.3:-76070342 None:intergenic
TTCTGATCCGGATCCTGTTA+TGG 0.323739 1.3:+76070224 MS.gene74967:CDS
GTGAATTCAAATGTGTTTCC+AGG 0.358885 1.3:+76070381 MS.gene74967:CDS
CCAGGAAACTTCAAAGATAC+TGG 0.364740 1.3:+76070342 MS.gene74967:CDS
TTTGGAGAAAGGTGAGGTTA+TGG 0.393881 1.3:+76070563 MS.gene74967:CDS
TGAAAATGCACAAGTCCTCT+TGG 0.414763 1.3:-76070591 None:intergenic
GCTATGCAGTAATCCATAAC+AGG 0.434646 1.3:-76070237 None:intergenic
ATAGCAACTTCTGTTGCTCT+TGG 0.444865 1.3:-76070477 None:intergenic
CAGTAATCCATAACAGGATC+CGG 0.469062 1.3:-76070231 None:intergenic
TTTCCAGGTTTAAACACTCT+TGG 0.469660 1.3:+76070396 MS.gene74967:CDS
CAGCAATGATGAAACACATA+AGG 0.504374 1.3:-76070194 None:intergenic
ACACATAAGGACTAAAAGAA+TGG 0.521199 1.3:-76070181 None:intergenic
AGAAGTTGCTATTGTGTTGG+AGG 0.523101 1.3:+76070488 MS.gene74967:CDS
AACAGAAGTTGCTATTGTGT+TGG 0.541553 1.3:+76070485 MS.gene74967:CDS
GAAGTTGCTATTGTGTTGGA+GGG 0.567803 1.3:+76070489 MS.gene74967:CDS
ACTCCAAGAGTGTTTAAACC+TGG 0.567991 1.3:-76070399 None:intergenic
GAAATCTTCAACTGTTGCTG+TGG 0.571728 1.3:-76070298 None:intergenic
AGCTTTCTAAGTTAAAGAAG+AGG 0.576087 1.3:+76070757 MS.gene74967:CDS
GTTGCTCTTGGATGAAAGTG+AGG 0.618955 1.3:-76070465 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAATTCCAAATGTTGTTTT+TGG + chr1.3:76070681-76070700 MS.gene74967:CDS 20.0%
!! CAAGATTTTTGCTAAAGTTT+TGG + chr1.3:76070545-76070564 MS.gene74967:CDS 25.0%
!!! GAATTTGTTATCTTCTGATT+TGG - chr1.3:76070262-76070281 None:intergenic 25.0%
AAACTACCAAAAATAGTAGC+AGG - chr1.3:76070636-76070655 None:intergenic 30.0%
ACACATAAGGACTAAAAGAA+TGG - chr1.3:76070184-76070203 None:intergenic 30.0%
AGCTTTCTAAGTTAAAGAAG+AGG + chr1.3:76070757-76070776 MS.gene74967:CDS 30.0%
TGAGATCCAAAAACAACATT+TGG - chr1.3:76070690-76070709 None:intergenic 30.0%
TTGGAGGGAAAAATTTATTC+AGG + chr1.3:76070504-76070523 MS.gene74967:CDS 30.0%
! AGATACTGGATTTTCTTCAA+TGG + chr1.3:76070356-76070375 MS.gene74967:CDS 30.0%
! AGCTTTTGGACTTAATTCTA+AGG + chr1.3:76070734-76070753 MS.gene74967:CDS 30.0%
! GATACTGGATTTTCTTCAAT+GGG + chr1.3:76070357-76070376 MS.gene74967:CDS 30.0%
! TCAGATTAAAGATGAGCTTT+TGG + chr1.3:76070707-76070726 MS.gene74967:CDS 30.0%
!! AGTTTTGATAGTCAAAATCC+TGG + chr1.3:76070651-76070670 MS.gene74967:CDS 30.0%
!! TTTGGAATTTTCATCAAACC+AGG - chr1.3:76070672-76070691 None:intergenic 30.0%
!! TTTTCTGGTATAAAACTACC+AGG + chr1.3:76070324-76070343 MS.gene74967:CDS 30.0%
!!! GTTGAAGATTTCACTTTTTC+TGG + chr1.3:76070309-76070328 MS.gene74967:CDS 30.0%
!!! TTTGCTAAAGTTTTGGAGAA+AGG + chr1.3:76070552-76070571 MS.gene74967:CDS 30.0%
CAGCAATGATGAAACACATA+AGG - chr1.3:76070197-76070216 None:intergenic 35.0%
GTGAATTCAAATGTGTTTCC+AGG + chr1.3:76070381-76070400 MS.gene74967:CDS 35.0%
TTTCCAGGTTTAAACACTCT+TGG + chr1.3:76070396-76070415 MS.gene74967:CDS 35.0%
! AACAGAAGTTGCTATTGTGT+TGG + chr1.3:76070485-76070504 MS.gene74967:CDS 35.0%
! GATGTTCCTGCTACTATTTT+TGG + chr1.3:76070627-76070646 MS.gene74967:CDS 35.0%
! GTGCATTTTCAGATGAATGT+TGG + chr1.3:76070603-76070622 MS.gene74967:CDS 35.0%
!! GAGCTTTTGGAAAAAGCTTT+TGG + chr1.3:76070720-76070739 MS.gene74967:CDS 35.0%
!! GTTAGAGCAGATTTTGATGT+TGG + chr1.3:76070429-76070448 MS.gene74967:CDS 35.0%
!!! TAAAGTTTTGGAGAAAGGTG+AGG + chr1.3:76070557-76070576 MS.gene74967:CDS 35.0%
ATAGCAACTTCTGTTGCTCT+TGG - chr1.3:76070480-76070499 None:intergenic 40.0%
CAGTAATCCATAACAGGATC+CGG - chr1.3:76070234-76070253 None:intergenic 40.0%
CCAGGAAACTTCAAAGATAC+TGG + chr1.3:76070342-76070361 MS.gene74967:CDS 40.0%
GAAATCTTCAACTGTTGCTG+TGG - chr1.3:76070301-76070320 None:intergenic 40.0%
GCTATGCAGTAATCCATAAC+AGG - chr1.3:76070240-76070259 None:intergenic 40.0%
TGAAAATGCACAAGTCCTCT+TGG - chr1.3:76070594-76070613 None:intergenic 40.0%
TTTGGAGAAAGGTGAGGTTA+TGG + chr1.3:76070563-76070582 MS.gene74967:CDS 40.0%
! AGAAGTTGCTATTGTGTTGG+AGG + chr1.3:76070488-76070507 MS.gene74967:CDS 40.0%
! GAAGTTGCTATTGTGTTGGA+GGG + chr1.3:76070489-76070508 MS.gene74967:CDS 40.0%
! GAGGTTATGGTTTTTCCAAG+AGG + chr1.3:76070576-76070595 MS.gene74967:CDS 40.0%
!! ACTCCAAGAGTGTTTAAACC+TGG - chr1.3:76070402-76070421 None:intergenic 40.0%
!! AGAGCAGATTTTGATGTTGG+TGG + chr1.3:76070432-76070451 MS.gene74967:CDS 40.0%
!! CCAGTATCTTTGAAGTTTCC+TGG - chr1.3:76070345-76070364 None:intergenic 40.0%
!!! TGCTGCTTTTTGTTCTGATC+CGG + chr1.3:76070212-76070231 MS.gene74967:CDS 40.0%
GTTGCTCTTGGATGAAAGTG+AGG - chr1.3:76070468-76070487 None:intergenic 45.0%
TTCTGATCCGGATCCTGTTA+TGG + chr1.3:76070224-76070243 MS.gene74967:CDS 45.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 76070162 76070794 76070162 ID=MS.gene74967
chr1.3 mRNA 76070162 76070794 76070162 ID=MS.gene74967.t1;Parent=MS.gene74967
chr1.3 exon 76070162 76070794 76070162 ID=MS.gene74967.t1.exon1;Parent=MS.gene74967.t1
chr1.3 CDS 76070162 76070794 76070162 ID=cds.MS.gene74967.t1;Parent=MS.gene74967.t1
Gene Sequence

>MS.gene74967

ATGATCAACAACAAAGTAACCATTCTTTTAGTCCTTATGTGTTTCATCATTGCTGCTTTTTGTTCTGATCCGGATCCTGTTATGGATTACTGCATAGCCAAATCAGAAGATAACAAATTCAGTTGCAAAAACTCATCCACAGCAACAGTTGAAGATTTCACTTTTTCTGGTATAAAACTACCAGGAAACTTCAAAGATACTGGATTTTCTTCAATGGGAGTGAATTCAAATGTGTTTCCAGGTTTAAACACTCTTGGAGTTTCATTTGTTAGAGCAGATTTTGATGTTGGTGGTGTTAATGTTCCTCACTTTCATCCAAGAGCAACAGAAGTTGCTATTGTGTTGGAGGGAAAAATTTATTCAGGTTTTGTTGATACTAAGAACAAGATTTTTGCTAAAGTTTTGGAGAAAGGTGAGGTTATGGTTTTTCCAAGAGGACTTGTGCATTTTCAGATGAATGTTGGTGATGTTCCTGCTACTATTTTTGGTAGTTTTGATAGTCAAAATCCTGGTTTGATGAAAATTCCAAATGTTGTTTTTGGATCTCAGATTAAAGATGAGCTTTTGGAAAAAGCTTTTGGACTTAATTCTAAGGAGCTTTCTAAGTTAAAGAAGAGGTTTACTCCTAGCTAA

Protein sequence

>MS.gene74967.t1

MINNKVTILLVLMCFIIAAFCSDPDPVMDYCIAKSEDNKFSCKNSSTATVEDFTFSGIKLPGNFKDTGFSSMGVNSNVFPGLNTLGVSFVRADFDVGGVNVPHFHPRATEVAIVLEGKIYSGFVDTKNKIFAKVLEKGEVMVFPRGLVHFQMNVGDVPATIFGSFDSQNPGLMKIPNVVFGSQIKDELLEKAFGLNSKELSKLKKRFTPS