Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76423.t1 | XP_013446394.1 | 95.1 | 123 | 6 | 0 | 1 | 123 | 1 | 123 | 3.90E-59 | 237.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76423.t1 | A0A072TTD9 | 95.1 | 123 | 6 | 0 | 1 | 123 | 1 | 123 | 2.8e-59 | 237.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052053 | MS.gene76423 | 0.840235 | 9.83E-58 | -1.69E-46 |
| MS.gene052091 | MS.gene76423 | 0.803532 | 3.33E-49 | -1.69E-46 |
| MS.gene052570 | MS.gene76423 | 0.800257 | 1.57E-48 | -1.69E-46 |
| MS.gene053932 | MS.gene76423 | 0.832285 | 1.03E-55 | -1.69E-46 |
| MS.gene055549 | MS.gene76423 | 0.829362 | 5.37E-55 | -1.69E-46 |
| MS.gene055550 | MS.gene76423 | 0.836633 | 8.35E-57 | -1.69E-46 |
| MS.gene055551 | MS.gene76423 | 0.822046 | 2.92E-53 | -1.69E-46 |
| MS.gene056365 | MS.gene76423 | 0.811874 | 5.63E-51 | -1.69E-46 |
| MS.gene056530 | MS.gene76423 | 0.80068 | 1.29E-48 | -1.69E-46 |
| MS.gene06023 | MS.gene76423 | 0.834837 | 2.38E-56 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76423.t1 | MTR_8g077145 | 95.122 | 123 | 6 | 0 | 1 | 123 | 1 | 123 | 4.45e-84 | 241 |
| MS.gene76423.t1 | MTR_5g020860 | 60.630 | 127 | 44 | 3 | 1 | 123 | 1 | 125 | 6.74e-47 | 147 |
| MS.gene76423.t1 | MTR_7g026910 | 42.857 | 91 | 29 | 4 | 1 | 89 | 1 | 70 | 3.44e-11 | 55.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene76423.t1 | AT2G18210 | 48.062 | 129 | 54 | 6 | 1 | 123 | 3 | 124 | 1.83e-25 | 93.2 |
| MS.gene76423.t1 | AT4G36500 | 46.341 | 123 | 63 | 3 | 1 | 123 | 3 | 122 | 5.62e-22 | 84.3 |
| MS.gene76423.t1 | AT3G50900 | 40.602 | 133 | 50 | 6 | 1 | 123 | 1 | 114 | 5.59e-21 | 81.6 |
| MS.gene76423.t1 | AT5G66490 | 36.923 | 130 | 54 | 6 | 1 | 123 | 1 | 109 | 7.14e-14 | 63.2 |
Find 26 sgRNAs with CRISPR-Local
Find 32 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAATAGATAACTGGTTTAT+TGG | 0.180498 | 8.1:-30613668 | None:intergenic |
| ATGACTCTAGGTTCAACTTT+TGG | 0.219816 | 8.1:+30613472 | MS.gene76423:CDS |
| TCATCCACTTCTCAACTTCT+TGG | 0.245054 | 8.1:+30613555 | MS.gene76423:CDS |
| TCAATATACCTTGCAAATTC+AGG | 0.324241 | 8.1:-30613610 | None:intergenic |
| TATCACTTGTAATAGATAAC+TGG | 0.340382 | 8.1:-30613676 | None:intergenic |
| TTGACAACTCGGAGAGGCCT+TGG | 0.364992 | 8.1:+30613337 | MS.gene76423:CDS |
| TTACTATTAACCTTCTTTGT+GGG | 0.373064 | 8.1:-30613517 | None:intergenic |
| ACTCGGAGAGGCCTTGGAAA+TGG | 0.385033 | 8.1:+30613343 | MS.gene76423:CDS |
| ATTACTATTAACCTTCTTTG+TGG | 0.400104 | 8.1:-30613518 | None:intergenic |
| CTCGGAGAGGCCTTGGAAAT+GGG | 0.438584 | 8.1:+30613344 | MS.gene76423:CDS |
| AAGGTATATTGAGTATCTTA+AGG | 0.468319 | 8.1:+30613621 | MS.gene76423:CDS |
| ATGGGAGATATGAGAGACTA+GGG | 0.472587 | 8.1:+30613362 | MS.gene76423:CDS |
| GAAGTATTGGATGCTCTAGT+AGG | 0.500496 | 8.1:-30613442 | None:intergenic |
| AATGGGAGATATGAGAGACT+AGG | 0.509338 | 8.1:+30613361 | MS.gene76423:CDS |
| TACTATTAACCTTCTTTGTG+GGG | 0.511877 | 8.1:-30613516 | None:intergenic |
| TATATTGAGTATCTTAAGGA+AGG | 0.518072 | 8.1:+30613625 | MS.gene76423:CDS |
| AAATCCAAGAAGTTGAGAAG+TGG | 0.550501 | 8.1:-30613559 | None:intergenic |
| TGTTTAGATCTTTGACAACT+CGG | 0.569650 | 8.1:+30613326 | MS.gene76423:CDS |
| ATCTTAAGGAAGGAGGCATG+TGG | 0.588582 | 8.1:+30613635 | MS.gene76423:CDS |
| TCTCATATCTCCCATTTCCA+AGG | 0.611655 | 8.1:-30613354 | None:intergenic |
| CAGCTAGGCCTGAATTTGCA+AGG | 0.622241 | 8.1:+30613602 | MS.gene76423:CDS |
| ATTGAGTATCTTAAGGAAGG+AGG | 0.641255 | 8.1:+30613628 | MS.gene76423:CDS |
| CTCATCATGCAAAAGTGTTG+TGG | 0.641475 | 8.1:-30613398 | None:intergenic |
| TCTTAAGGAAGGAGGCATGT+GGG | 0.644106 | 8.1:+30613636 | MS.gene76423:CDS |
| AGATCTTTGACAACTCGGAG+AGG | 0.670905 | 8.1:+30613331 | MS.gene76423:CDS |
| AGTTGTCAAAGATCTAAACA+TGG | 0.718860 | 8.1:-30613323 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGGTATATTGAGTATCTTA+AGG | + | chr8.1:30613621-30613640 | MS.gene76423:CDS | 25.0% |
| ! | ATTACTATTAACCTTCTTTG+TGG | - | chr8.1:30613521-30613540 | None:intergenic | 25.0% |
| ! | TATATTGAGTATCTTAAGGA+AGG | + | chr8.1:30613625-30613644 | MS.gene76423:CDS | 25.0% |
| ! | TTACTATTAACCTTCTTTGT+GGG | - | chr8.1:30613520-30613539 | None:intergenic | 25.0% |
| !! | GTAATAGATAACTGGTTTAT+TGG | - | chr8.1:30613671-30613690 | None:intergenic | 25.0% |
| !!! | AGTCATTTTTCTTGAAGTAT+TGG | - | chr8.1:30613458-30613477 | None:intergenic | 25.0% |
| ACTTCAAGAAAAATGACTCT+AGG | + | chr8.1:30613460-30613479 | MS.gene76423:CDS | 30.0% | |
| TACTATTAACCTTCTTTGTG+GGG | - | chr8.1:30613519-30613538 | None:intergenic | 30.0% | |
| TCAATATACCTTGCAAATTC+AGG | - | chr8.1:30613613-30613632 | None:intergenic | 30.0% | |
| TGTTTAGATCTTTGACAACT+CGG | + | chr8.1:30613326-30613345 | MS.gene76423:CDS | 30.0% | |
| AAAAAGAAACCCCACAAAGA+AGG | + | chr8.1:30613507-30613526 | MS.gene76423:CDS | 35.0% | |
| AAATCCAAGAAGTTGAGAAG+TGG | - | chr8.1:30613562-30613581 | None:intergenic | 35.0% | |
| ATTGAGTATCTTAAGGAAGG+AGG | + | chr8.1:30613628-30613647 | MS.gene76423:CDS | 35.0% | |
| GGAAAAAGAAAACAACAGCT+AGG | + | chr8.1:30613587-30613606 | MS.gene76423:CDS | 35.0% | |
| ! | ATGACTCTAGGTTCAACTTT+TGG | + | chr8.1:30613472-30613491 | MS.gene76423:CDS | 35.0% |
| ! | TCAACTTCTTGGATTTTCGT+AGG | + | chr8.1:30613566-30613585 | MS.gene76423:CDS | 35.0% |
| ! | TCTTTGTGGGGTTTCTTTTT+AGG | - | chr8.1:30613507-30613526 | None:intergenic | 35.0% |
| ! | TGACTCTAGGTTCAACTTTT+GGG | + | chr8.1:30613473-30613492 | MS.gene76423:CDS | 35.0% |
| AATGGGAGATATGAGAGACT+AGG | + | chr8.1:30613361-30613380 | MS.gene76423:CDS | 40.0% | |
| ATGGGAGATATGAGAGACTA+GGG | + | chr8.1:30613362-30613381 | MS.gene76423:CDS | 40.0% | |
| TCATCCACTTCTCAACTTCT+TGG | + | chr8.1:30613555-30613574 | MS.gene76423:CDS | 40.0% | |
| TCTCATATCTCCCATTTCCA+AGG | - | chr8.1:30613357-30613376 | None:intergenic | 40.0% | |
| ! | GACTCTAGGTTCAACTTTTG+GGG | + | chr8.1:30613474-30613493 | MS.gene76423:CDS | 40.0% |
| !! | CTCATCATGCAAAAGTGTTG+TGG | - | chr8.1:30613401-30613420 | None:intergenic | 40.0% |
| !! | GAAGTATTGGATGCTCTAGT+AGG | - | chr8.1:30613445-30613464 | None:intergenic | 40.0% |
| AGATCTTTGACAACTCGGAG+AGG | + | chr8.1:30613331-30613350 | MS.gene76423:CDS | 45.0% | |
| ! | ATCTTAAGGAAGGAGGCATG+TGG | + | chr8.1:30613635-30613654 | MS.gene76423:CDS | 45.0% |
| ! | TCTTAAGGAAGGAGGCATGT+GGG | + | chr8.1:30613636-30613655 | MS.gene76423:CDS | 45.0% |
| CAGCTAGGCCTGAATTTGCA+AGG | + | chr8.1:30613602-30613621 | MS.gene76423:CDS | 50.0% | |
| ACTCGGAGAGGCCTTGGAAA+TGG | + | chr8.1:30613343-30613362 | MS.gene76423:CDS | 55.0% | |
| CTCGGAGAGGCCTTGGAAAT+GGG | + | chr8.1:30613344-30613363 | MS.gene76423:CDS | 55.0% | |
| TTGACAACTCGGAGAGGCCT+TGG | + | chr8.1:30613337-30613356 | MS.gene76423:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 30613325 | 30613696 | 30613325 | ID=MS.gene76423 |
| chr8.1 | mRNA | 30613325 | 30613696 | 30613325 | ID=MS.gene76423.t1;Parent=MS.gene76423 |
| chr8.1 | exon | 30613325 | 30613696 | 30613325 | ID=MS.gene76423.t1.exon1;Parent=MS.gene76423.t1 |
| chr8.1 | CDS | 30613325 | 30613696 | 30613325 | ID=cds.MS.gene76423.t1;Parent=MS.gene76423.t1 |
| Gene Sequence |
| Protein sequence |
