Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77304.t1 | XP_003626285.1 | 67.9 | 168 | 13 | 2 | 1 | 138 | 274 | 430 | 2.40E-50 | 208.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77304.t1 | Q940P8 | 60.7 | 168 | 25 | 2 | 1 | 138 | 274 | 430 | 4.1e-46 | 185.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77304.t1 | G7KYN5 | 67.9 | 168 | 13 | 2 | 1 | 138 | 274 | 430 | 1.7e-50 | 208.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049825 | MS.gene77304 | 0.803175 | 3.95E-49 | -1.69E-46 |
| MS.gene049873 | MS.gene77304 | 0.819386 | 1.19E-52 | -1.69E-46 |
| MS.gene051594 | MS.gene77304 | 0.821793 | 3.34E-53 | -1.69E-46 |
| MS.gene052519 | MS.gene77304 | 0.811878 | 5.62E-51 | -1.69E-46 |
| MS.gene052584 | MS.gene77304 | 0.805094 | 1.58E-49 | -1.69E-46 |
| MS.gene053226 | MS.gene77304 | 0.824777 | 6.72E-54 | -1.69E-46 |
| MS.gene057060 | MS.gene77304 | 0.806043 | 9.97E-50 | -1.69E-46 |
| MS.gene057314 | MS.gene77304 | 0.806266 | 8.94E-50 | -1.69E-46 |
| MS.gene058625 | MS.gene77304 | 0.803334 | 3.66E-49 | -1.69E-46 |
| MS.gene05954 | MS.gene77304 | 0.825293 | 5.07E-54 | -1.69E-46 |
| MS.gene059625 | MS.gene77304 | 0.805441 | 1.33E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77304.t1 | MTR_7g067470 | 67.857 | 168 | 13 | 2 | 1 | 138 | 274 | 430 | 1.12e-67 | 214 |
| MS.gene77304.t1 | MTR_7g113470 | 67.857 | 168 | 13 | 2 | 1 | 138 | 274 | 430 | 1.18e-67 | 214 |
| MS.gene77304.t1 | MTR_3g465910 | 72.727 | 55 | 12 | 1 | 87 | 138 | 453 | 507 | 5.00e-17 | 77.4 |
| MS.gene77304.t1 | MTR_5g087560 | 31.746 | 126 | 75 | 1 | 12 | 137 | 322 | 436 | 2.00e-12 | 63.9 |
| MS.gene77304.t1 | MTR_3g109925 | 37.113 | 97 | 49 | 3 | 34 | 129 | 292 | 377 | 4.07e-11 | 60.1 |
| MS.gene77304.t1 | MTR_3g109925 | 37.113 | 97 | 49 | 3 | 34 | 129 | 349 | 434 | 4.51e-11 | 60.1 |
| MS.gene77304.t1 | MTR_8g105970 | 31.967 | 122 | 67 | 3 | 34 | 149 | 206 | 317 | 8.41e-11 | 59.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene77304.t1 | AT5G20890 | 61.212 | 165 | 23 | 2 | 4 | 138 | 277 | 430 | 4.99e-57 | 186 |
| MS.gene77304.t1 | AT3G11830 | 31.452 | 124 | 74 | 1 | 14 | 137 | 324 | 436 | 4.46e-11 | 60.1 |
| MS.gene77304.t1 | AT3G18190 | 33.884 | 121 | 65 | 2 | 34 | 149 | 353 | 463 | 7.87e-11 | 59.7 |
Find 42 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTTGCCATTACCATTTC+AGG | 0.181073 | 7.2:-36176654 | None:intergenic |
| CTTATCGAAGATATTATTAT+TGG | 0.183032 | 7.2:+36175847 | MS.gene77304:CDS |
| GAGCGTCTGGCTCTGGTAAC+TGG | 0.301470 | 7.2:+36175766 | MS.gene77304:CDS |
| GGCAAAGGAAATTGATTCAT+TGG | 0.322602 | 7.2:+36176668 | MS.gene77304:CDS |
| GCATTATCAGCGATGGTTGT+TGG | 0.348817 | 7.2:-36176760 | None:intergenic |
| GAAAAGATGAACAAGATAAT+AGG | 0.380452 | 7.2:+36175724 | None:intergenic |
| CATTATCAGCGATGGTTGTT+GGG | 0.398556 | 7.2:-36176759 | None:intergenic |
| TGCCCAAGCTTAACAGACTC+TGG | 0.403665 | 7.2:-36175817 | None:intergenic |
| GGCACTTTCCCAAGCACTCT+TGG | 0.419611 | 7.2:+36176732 | MS.gene77304:CDS |
| TGGAGGCGGGTGGCCTGAAA+TGG | 0.444431 | 7.2:+36176641 | MS.gene77304:CDS |
| TTGGGATAGCCAAGAGTGCT+TGG | 0.451084 | 7.2:-36176741 | None:intergenic |
| TTCTGGTGTTGCAATGTGGC+AGG | 0.456604 | 7.2:+36175891 | MS.gene77304:CDS |
| AATGACAGTAGGTTGTTACT+TGG | 0.472228 | 7.2:+36176621 | MS.gene77304:CDS |
| AACCCAGAGTCTGTTAAGCT+TGG | 0.477537 | 7.2:+36175814 | MS.gene77304:CDS |
| ACCCAGAGTCTGTTAAGCTT+GGG | 0.494078 | 7.2:+36175815 | MS.gene77304:CDS |
| GCATTTGAGCTGAACATGTA+TGG | 0.504914 | 7.2:+36176423 | MS.gene77304:CDS |
| AGTAGGTTGTTACTTGGAGG+CGG | 0.510127 | 7.2:+36176627 | MS.gene77304:CDS |
| AAGTAACAACCTACTGTCAT+TGG | 0.511750 | 7.2:-36176619 | None:intergenic |
| GACAGTAGGTTGTTACTTGG+AGG | 0.514052 | 7.2:+36176624 | MS.gene77304:CDS |
| CGAAGATATTATTATTGGTG+AGG | 0.514475 | 7.2:+36175852 | MS.gene77304:CDS |
| TGGGATAGCCAAGAGTGCTT+GGG | 0.517026 | 7.2:-36176740 | None:intergenic |
| AGGAAATTGATTCATTGGCT+AGG | 0.530474 | 7.2:+36176673 | MS.gene77304:CDS |
| GCCCAAGCTTAACAGACTCT+GGG | 0.530545 | 7.2:-36175816 | None:intergenic |
| CGGGTGGCCTGAAATGGTAA+TGG | 0.539649 | 7.2:+36176647 | MS.gene77304:CDS |
| AACAGACTCTGGGTTGTCAA+AGG | 0.543890 | 7.2:-36175806 | None:intergenic |
| GAGCCAGACGCTCAAAACAT+TGG | 0.559739 | 7.2:-36175756 | None:intergenic |
| AAGTCTCTTGCAAATGGAGG+AGG | 0.561240 | 7.2:+36176711 | MS.gene77304:CDS |
| GTAGGTTGTTACTTGGAGGC+GGG | 0.563910 | 7.2:+36176628 | MS.gene77304:CDS |
| GCCTGAAATGGTAATGGCAA+AGG | 0.576296 | 7.2:+36176653 | MS.gene77304:CDS |
| TAACAACCTACTGTCATTGG+CGG | 0.584745 | 7.2:-36176616 | None:intergenic |
| GGTTGTTACTTGGAGGCGGG+TGG | 0.585391 | 7.2:+36176631 | MS.gene77304:CDS |
| ATGAACAAGATAATAGGTCA+TGG | 0.590525 | 7.2:+36175730 | MS.gene77304:CDS |
| TCATTGGCTAGGAAAACTCC+AGG | 0.591835 | 7.2:+36176684 | MS.gene77304:CDS |
| CAAACCAGCATTATCAGCGA+TGG | 0.610258 | 7.2:-36176767 | None:intergenic |
| CAGCCACCATGTTCTTGATG+AGG | 0.618440 | 7.2:+36176551 | MS.gene77304:intron |
| TCTCAGCCTCATCAAGAACA+TGG | 0.644225 | 7.2:-36176557 | None:intergenic |
| CTCAGACCGCCAATGACAGT+AGG | 0.655775 | 7.2:+36176610 | MS.gene77304:CDS |
| ATGTTCTTGATGAGGCTGAG+AGG | 0.658084 | 7.2:+36176559 | MS.gene77304:CDS |
| CGTCTGGCTCTGGTAACTGG+TGG | 0.662358 | 7.2:+36175769 | MS.gene77304:CDS |
| CAGCCTCATCAAGAACATGG+TGG | 0.689750 | 7.2:-36176554 | None:intergenic |
| GGTCTGAGATAGCACACACA+AGG | 0.715225 | 7.2:-36176595 | None:intergenic |
| GCATGCACAATAGTTCTGAG+AGG | 0.722642 | 7.2:+36175913 | MS.gene77304:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGCAGTAATTATTTTTTAAA+AGG | + | chr7.2:36176243-36176262 | MS.gene77304:intron | 15.0% |
| !! | CTTATCGAAGATATTATTAT+TGG | + | chr7.2:36175847-36175866 | MS.gene77304:CDS | 20.0% |
| !! | TATGTTTCATTATCTATTTG+TGG | + | chr7.2:36176082-36176101 | MS.gene77304:intron | 20.0% |
| !! | TCATAGTATTAGTAATTGAT+AGG | + | chr7.2:36176109-36176128 | MS.gene77304:intron | 20.0% |
| !!! | GATATATTGATTAAGTTTTC+TGG | + | chr7.2:36175874-36175893 | MS.gene77304:CDS | 20.0% |
| ! | GATGTTCATTTACATATTCA+AGG | + | chr7.2:36175983-36176002 | MS.gene77304:intron | 25.0% |
| ! | TATGTAAATGAACATCAGAA+AGG | - | chr7.2:36175979-36175998 | None:intergenic | 25.0% |
| !!! | TCATAGCATTTTTTCTTTTG+AGG | + | chr7.2:36176031-36176050 | MS.gene77304:intron | 25.0% |
| ACTGACTAGACCATAAAAAA+TGG | - | chr7.2:36176403-36176422 | None:intergenic | 30.0% | |
| ATGAACAAGATAATAGGTCA+TGG | + | chr7.2:36175730-36175749 | MS.gene77304:CDS | 30.0% | |
| ATTGAGTCCAATGATTTAAC+TGG | + | chr7.2:36176287-36176306 | MS.gene77304:intron | 30.0% | |
| CAGCATAGAAACTGAATATA+AGG | - | chr7.2:36176155-36176174 | None:intergenic | 30.0% | |
| CGAAGATATTATTATTGGTG+AGG | + | chr7.2:36175852-36175871 | MS.gene77304:CDS | 30.0% | |
| CTAGACCATAAAAAATGGTA+AGG | - | chr7.2:36176398-36176417 | None:intergenic | 30.0% | |
| CTTATATTCAGTTTCTATGC+TGG | + | chr7.2:36176153-36176172 | MS.gene77304:intron | 30.0% | |
| !! | TTTCTATGCTGGTTTATTGA+AGG | + | chr7.2:36176164-36176183 | MS.gene77304:intron | 30.0% |
| !!! | CTTTTGAGGTTTTCTTATTC+TGG | + | chr7.2:36176045-36176064 | MS.gene77304:intron | 30.0% |
| AAAAAGTCTCTTGCAAATGG+AGG | + | chr7.2:36176708-36176727 | MS.gene77304:CDS | 35.0% | |
| AAGTAACAACCTACTGTCAT+TGG | - | chr7.2:36176622-36176641 | None:intergenic | 35.0% | |
| AATGACAGTAGGTTGTTACT+TGG | + | chr7.2:36176621-36176640 | MS.gene77304:CDS | 35.0% | |
| CATAAAAAATGGTAAGGAGC+TGG | - | chr7.2:36176392-36176411 | None:intergenic | 35.0% | |
| GGAAAAAAGTCTCTTGCAAA+TGG | + | chr7.2:36176705-36176724 | MS.gene77304:CDS | 35.0% | |
| ! | AGGAAATTGATTCATTGGCT+AGG | + | chr7.2:36176673-36176692 | MS.gene77304:CDS | 35.0% |
| ! | GGCAAAGGAAATTGATTCAT+TGG | + | chr7.2:36176668-36176687 | MS.gene77304:CDS | 35.0% |
| !!! | CAGCTCCTTACCATTTTTTA+TGG | + | chr7.2:36176390-36176409 | MS.gene77304:intron | 35.0% |
| !!! | TTTGCAAGAGACTTTTTTCC+TGG | - | chr7.2:36176705-36176724 | None:intergenic | 35.0% |
| CAGCAGTCCAGTTAAATCAT+TGG | - | chr7.2:36176297-36176316 | None:intergenic | 40.0% | |
| CTGTGACAAAAACAAACGTC+AGG | - | chr7.2:36176534-36176553 | None:intergenic | 40.0% | |
| GCATTTGAGCTGAACATGTA+TGG | + | chr7.2:36176423-36176442 | MS.gene77304:CDS | 40.0% | |
| GTAAGGAGCTGGTGAATATA+AGG | - | chr7.2:36176381-36176400 | None:intergenic | 40.0% | |
| TAACAACCTACTGTCATTGG+CGG | - | chr7.2:36176619-36176638 | None:intergenic | 40.0% | |
| TAACTGGACTGCTGAAATGT+TGG | + | chr7.2:36176303-36176322 | MS.gene77304:intron | 40.0% | |
| TCCTTTGCCATTACCATTTC+AGG | - | chr7.2:36176657-36176676 | None:intergenic | 40.0% | |
| !!! | AGTTTTCTGGTGTTGCAATG+TGG | + | chr7.2:36175887-36175906 | MS.gene77304:CDS | 40.0% |
| AACAGACTCTGGGTTGTCAA+AGG | - | chr7.2:36175809-36175828 | None:intergenic | 45.0% | |
| AACCCAGAGTCTGTTAAGCT+TGG | + | chr7.2:36175814-36175833 | MS.gene77304:CDS | 45.0% | |
| AAGTCTCTTGCAAATGGAGG+AGG | + | chr7.2:36176711-36176730 | MS.gene77304:CDS | 45.0% | |
| ACCCAGAGTCTGTTAAGCTT+GGG | + | chr7.2:36175815-36175834 | MS.gene77304:CDS | 45.0% | |
| ATGTTCTTGATGAGGCTGAG+AGG | + | chr7.2:36176559-36176578 | MS.gene77304:CDS | 45.0% | |
| TCTCAGCCTCATCAAGAACA+TGG | - | chr7.2:36176560-36176579 | None:intergenic | 45.0% | |
| ! | AGTAGGTTGTTACTTGGAGG+CGG | + | chr7.2:36176627-36176646 | MS.gene77304:CDS | 45.0% |
| ! | GACAGTAGGTTGTTACTTGG+AGG | + | chr7.2:36176624-36176643 | MS.gene77304:CDS | 45.0% |
| ! | TCATTGGCTAGGAAAACTCC+AGG | + | chr7.2:36176684-36176703 | MS.gene77304:CDS | 45.0% |
| !! | CATCCAATGTTTTGAGCGTC+TGG | + | chr7.2:36175753-36175772 | MS.gene77304:CDS | 45.0% |
| !! | GCATGCACAATAGTTCTGAG+AGG | + | chr7.2:36175913-36175932 | MS.gene77304:CDS | 45.0% |
| !! | GCCTGAAATGGTAATGGCAA+AGG | + | chr7.2:36176653-36176672 | MS.gene77304:CDS | 45.0% |
| CAGCCACCATGTTCTTGATG+AGG | + | chr7.2:36176551-36176570 | MS.gene77304:intron | 50.0% | |
| CAGCCTCATCAAGAACATGG+TGG | - | chr7.2:36176557-36176576 | None:intergenic | 50.0% | |
| GAGCCAGACGCTCAAAACAT+TGG | - | chr7.2:36175759-36175778 | None:intergenic | 50.0% | |
| GCCCAAGCTTAACAGACTCT+GGG | - | chr7.2:36175819-36175838 | None:intergenic | 50.0% | |
| TGCCCAAGCTTAACAGACTC+TGG | - | chr7.2:36175820-36175839 | None:intergenic | 50.0% | |
| ! | GGTCTGAGATAGCACACACA+AGG | - | chr7.2:36176598-36176617 | None:intergenic | 50.0% |
| ! | GTAGGTTGTTACTTGGAGGC+GGG | + | chr7.2:36176628-36176647 | MS.gene77304:CDS | 50.0% |
| !! | ATGTTTTGAGCGTCTGGCTC+TGG | + | chr7.2:36175759-36175778 | MS.gene77304:CDS | 50.0% |
| !! | TGGGATAGCCAAGAGTGCTT+GGG | - | chr7.2:36176743-36176762 | None:intergenic | 50.0% |
| !! | TTCTGGTGTTGCAATGTGGC+AGG | + | chr7.2:36175891-36175910 | MS.gene77304:CDS | 50.0% |
| !! | TTGGGATAGCCAAGAGTGCT+TGG | - | chr7.2:36176744-36176763 | None:intergenic | 50.0% |
| CGGGTGGCCTGAAATGGTAA+TGG | + | chr7.2:36176647-36176666 | MS.gene77304:CDS | 55.0% | |
| CTCAGACCGCCAATGACAGT+AGG | + | chr7.2:36176610-36176629 | MS.gene77304:CDS | 55.0% | |
| GGCACTTTCCCAAGCACTCT+TGG | + | chr7.2:36176732-36176751 | MS.gene77304:CDS | 55.0% | |
| !! | AGTTCTGAGAGGTGCGTGCT+AGG | + | chr7.2:36175924-36175943 | MS.gene77304:intron | 55.0% |
| ! | GGTTGTTACTTGGAGGCGGG+TGG | + | chr7.2:36176631-36176650 | MS.gene77304:CDS | 60.0% |
| !! | CGTCTGGCTCTGGTAACTGG+TGG | + | chr7.2:36175769-36175788 | MS.gene77304:CDS | 60.0% |
| !! | GAGCGTCTGGCTCTGGTAAC+TGG | + | chr7.2:36175766-36175785 | MS.gene77304:CDS | 60.0% |
| TGGAGGCGGGTGGCCTGAAA+TGG | + | chr7.2:36176641-36176660 | MS.gene77304:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 36175730 | 36176776 | 36175730 | ID=MS.gene77304 |
| chr7.2 | mRNA | 36175730 | 36176776 | 36175730 | ID=MS.gene77304.t1;Parent=MS.gene77304 |
| chr7.2 | exon | 36175730 | 36175934 | 36175730 | ID=MS.gene77304.t1.exon1;Parent=MS.gene77304.t1 |
| chr7.2 | CDS | 36175730 | 36175934 | 36175730 | ID=cds.MS.gene77304.t1;Parent=MS.gene77304.t1 |
| chr7.2 | exon | 36176418 | 36176457 | 36176418 | ID=MS.gene77304.t1.exon2;Parent=MS.gene77304.t1 |
| chr7.2 | CDS | 36176418 | 36176457 | 36176418 | ID=cds.MS.gene77304.t1;Parent=MS.gene77304.t1 |
| chr7.2 | exon | 36176554 | 36176776 | 36176554 | ID=MS.gene77304.t1.exon3;Parent=MS.gene77304.t1 |
| chr7.2 | CDS | 36176554 | 36176776 | 36176554 | ID=cds.MS.gene77304.t1;Parent=MS.gene77304.t1 |
| Gene Sequence |
| Protein sequence |
