AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene77959

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77959.t1 MTR_3g030540 93.005 386 27 0 1 386 52 437 0.0 728
MS.gene77959.t1 MTR_2g060350 67.196 378 120 3 12 386 67 443 0.0 526
MS.gene77959.t1 MTR_1g052315 55.615 374 165 1 12 384 57 430 4.00e-144 417
MS.gene77959.t1 MTR_7g091850 55.733 375 164 2 12 384 69 443 9.03e-140 405
MS.gene77959.t1 MTR_7g091840 55.031 318 139 2 15 328 61 378 5.11e-118 348
MS.gene77959.t1 MTR_2g103410 46.594 367 193 2 19 382 59 425 4.61e-111 332
MS.gene77959.t1 MTR_8g065030 46.802 344 181 2 19 360 65 408 2.13e-108 326
MS.gene77959.t1 MTR_6g005630 44.602 352 186 6 15 364 41 385 3.54e-97 296
MS.gene77959.t1 MTR_2g049400 42.276 369 203 8 18 383 27 388 2.78e-86 267
MS.gene77959.t1 MTR_4g084050 39.948 383 206 7 9 377 13 385 1.82e-85 265
MS.gene77959.t1 MTR_7g110050 39.833 359 196 6 20 363 68 421 1.27e-84 265
MS.gene77959.t1 MTR_3g084360 39.247 372 211 6 13 377 18 381 1.80e-83 259
MS.gene77959.t1 MTR_1g086390 40.214 373 206 8 19 377 81 450 2.22e-83 262
MS.gene77959.t1 MTR_3g084380 40.659 364 197 7 4 358 6 359 4.02e-83 259
MS.gene77959.t1 MTR_3g438050 42.075 347 189 8 17 357 25 365 1.07e-82 258
MS.gene77959.t1 MTR_6g005630 41.761 352 173 8 15 364 41 362 1.58e-82 258
MS.gene77959.t1 MTR_8g065010 38.583 381 198 7 11 357 94 472 2.52e-82 261
MS.gene77959.t1 MTR_3g054320 40.556 360 193 8 20 371 31 377 3.56e-82 257
MS.gene77959.t1 MTR_4g094715 40.960 354 188 7 19 357 65 412 5.01e-82 258
MS.gene77959.t1 MTR_4g083660 41.910 377 207 10 12 383 2 371 6.99e-82 255
MS.gene77959.t1 MTR_6g028080 39.837 369 196 8 18 373 29 384 5.81e-81 253
MS.gene77959.t1 MTR_5g033680 37.989 358 213 5 7 357 47 402 1.30e-80 255
MS.gene77959.t1 MTR_8g009810 41.333 375 209 9 16 385 32 400 2.05e-79 249
MS.gene77959.t1 MTR_5g043350 38.753 369 212 5 12 373 22 383 4.35e-79 249
MS.gene77959.t1 MTR_5g034090 37.565 386 205 9 5 357 103 485 4.81e-79 253
MS.gene77959.t1 MTR_7g113910 48.062 258 116 1 112 369 95 334 1.45e-78 246
MS.gene77959.t1 MTR_2g435640 38.606 373 212 11 16 378 50 415 2.51e-78 248
MS.gene77959.t1 MTR_2g103650 40.580 345 197 5 18 355 27 370 1.50e-77 245
MS.gene77959.t1 MTR_8g010080 39.665 358 196 7 20 373 32 373 2.79e-77 244
MS.gene77959.t1 MTR_1g088160 39.316 351 196 11 20 361 36 378 7.68e-77 243
MS.gene77959.t1 MTR_8g030930 40.431 371 200 10 1 362 15 373 2.20e-76 242
MS.gene77959.t1 MTR_4g120320 39.633 381 213 12 16 386 49 422 1.88e-74 238
MS.gene77959.t1 MTR_1g012390 38.525 366 208 9 15 372 24 380 1.04e-73 235
MS.gene77959.t1 MTR_2g103410 46.718 259 135 2 19 274 59 317 3.32e-72 229
MS.gene77959.t1 MTR_8g010090 38.095 357 200 8 20 371 32 372 1.96e-71 229
MS.gene77959.t1 MTR_2g058840 37.883 359 213 7 19 372 25 378 4.58e-71 229
MS.gene77959.t1 MTR_2g058670 37.845 362 211 8 19 372 25 380 4.63e-71 229
MS.gene77959.t1 MTR_8g010110 38.095 357 200 8 20 371 32 372 8.25e-71 227
MS.gene77959.t1 MTR_2g032710 38.701 354 204 6 15 357 63 414 1.87e-70 228
MS.gene77959.t1 MTR_8g010040 38.606 373 207 10 16 383 29 384 3.03e-70 226
MS.gene77959.t1 MTR_0157s0060 39.665 358 197 5 23 373 2 347 3.64e-70 224
MS.gene77959.t1 MTR_4g034020 39.665 358 197 5 23 373 2 347 3.64e-70 224
MS.gene77959.t1 MTR_1g013740 36.364 374 211 9 16 383 32 384 2.02e-69 223
MS.gene77959.t1 MTR_8g010070 38.227 361 199 12 20 373 32 375 1.06e-67 219
MS.gene77959.t1 MTR_2g058640 37.989 358 205 8 37 384 41 391 3.10e-67 218
MS.gene77959.t1 MTR_2g029170 38.209 335 197 9 52 383 31 358 1.11e-66 216
MS.gene77959.t1 MTR_8g008765 38.602 329 183 6 20 344 32 345 1.20e-66 215
MS.gene77959.t1 MTR_1g013730 35.389 373 214 9 16 382 24 375 4.14e-66 214
MS.gene77959.t1 MTR_7g035415 41.017 295 154 7 17 303 38 320 1.31e-65 212
MS.gene77959.t1 MTR_5g063430 37.459 307 178 6 9 310 15 312 5.46e-60 197
MS.gene77959.t1 MTR_5g034090 36.634 303 158 7 5 274 103 404 8.15e-58 194
MS.gene77959.t1 MTR_8g077320 39.735 302 137 7 17 303 31 302 9.10e-58 192
MS.gene77959.t1 MTR_8g008700 41.154 260 136 4 96 351 31 277 2.32e-57 189
MS.gene77959.t1 MTR_5g097860 34.056 323 202 7 17 333 23 340 4.72e-56 187
MS.gene77959.t1 MTR_8g010050 33.240 358 183 11 20 371 32 339 2.66e-50 172
MS.gene77959.t1 MTR_8g031180 46.073 191 98 1 157 342 70 260 5.21e-49 167
MS.gene77959.t1 MTR_2g100040 31.638 354 184 11 9 355 14 316 3.67e-42 151
MS.gene77959.t1 MTR_2g042740 28.834 326 197 11 84 383 128 444 2.44e-26 110
MS.gene77959.t1 MTR_4g124980 25.121 414 255 16 9 382 63 461 2.79e-23 101
MS.gene77959.t1 MTR_3g449590 25.000 396 257 12 9 372 37 424 2.44e-22 98.6
MS.gene77959.t1 MTR_1g086030 30.877 285 164 8 71 328 37 315 8.89e-22 96.3
MS.gene77959.t1 MTR_7g110710 29.268 328 192 14 86 386 117 431 9.58e-21 94.0
MS.gene77959.t1 MTR_7g110710 29.268 328 192 14 86 386 117 431 1.20e-20 93.6
MS.gene77959.t1 MTR_4g120730 27.551 294 188 9 105 382 143 427 1.39e-20 93.6
MS.gene77959.t1 MTR_1g086030 31.481 270 152 8 86 328 156 419 5.83e-20 91.7
MS.gene77959.t1 MTR_7g078680 24.615 325 216 8 83 384 126 444 3.44e-19 89.4
MS.gene77959.t1 MTR_5g090160 24.806 387 238 12 9 357 34 405 3.77e-19 89.0
MS.gene77959.t1 MTR_8g098840 24.907 269 178 8 104 357 146 405 2.43e-18 86.7
MS.gene77959.t1 MTR_8g028725 25.067 371 243 12 17 358 53 417 3.05e-18 86.7
MS.gene77959.t1 MTR_3g096330 26.578 301 184 10 105 384 179 463 4.49e-18 86.7
MS.gene77959.t1 MTR_7g088550 27.365 296 179 11 87 357 128 412 5.91e-18 85.5
MS.gene77959.t1 MTR_1g100060 25.253 297 188 10 86 357 167 454 1.80e-17 84.3
MS.gene77959.t1 MTR_1g100050 25.651 269 176 8 104 357 152 411 3.23e-17 83.6
MS.gene77959.t1 MTR_2g435340 25.312 320 205 9 76 377 51 354 2.57e-16 80.1
MS.gene77959.t1 MTR_2g435340 25.312 320 205 9 76 377 116 419 2.76e-16 80.5
MS.gene77959.t1 MTR_1g099850 24.262 305 195 11 86 363 129 424 9.98e-16 79.0
MS.gene77959.t1 MTR_1g100040 25.087 287 184 9 86 350 133 410 1.80e-15 78.2
MS.gene77959.t1 MTR_8g007320 49.153 59 29 1 313 371 22 79 4.76e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene77959.t1 AT3G07970 58.556 374 149 5 15 384 67 438 4.28e-152 437
MS.gene77959.t1 AT3G57510 52.291 371 166 5 18 384 67 430 3.29e-129 378
MS.gene77959.t1 AT2G41850 52.105 380 171 6 9 384 58 430 2.24e-119 353
MS.gene77959.t1 AT1G80170 46.933 375 193 4 13 382 47 420 6.99e-116 345
MS.gene77959.t1 AT1G23460 44.591 379 205 4 7 382 31 407 1.04e-107 323
MS.gene77959.t1 AT1G23460 44.591 379 205 4 7 382 55 431 2.40e-107 323
MS.gene77959.t1 AT1G70500 45.699 372 193 5 16 382 64 431 3.56e-106 320
MS.gene77959.t1 AT1G56710 42.553 376 184 9 7 357 29 397 1.92e-91 281
MS.gene77959.t1 AT5G14650 41.739 345 194 6 15 357 47 386 3.02e-88 273
MS.gene77959.t1 AT4G35670 42.857 364 193 7 20 377 25 379 1.36e-87 270
MS.gene77959.t1 AT2G43890 38.624 378 222 9 9 383 19 389 3.41e-86 267
MS.gene77959.t1 AT5G27530 42.618 359 183 6 20 372 54 395 1.94e-85 267
MS.gene77959.t1 AT5G44840 39.833 359 202 6 20 372 25 375 2.65e-84 262
MS.gene77959.t1 AT1G05660 39.418 378 218 9 9 382 18 388 4.75e-84 261
MS.gene77959.t1 AT3G15720 41.848 368 192 9 15 372 19 374 6.06e-84 263
MS.gene77959.t1 AT3G15720 41.848 368 192 9 15 372 20 375 6.30e-84 263
MS.gene77959.t1 AT4G18180 39.323 384 209 8 19 382 39 418 2.37e-83 260
MS.gene77959.t1 AT2G43870 41.489 376 205 13 15 384 17 383 7.16e-83 258
MS.gene77959.t1 AT1G05650 39.344 366 213 8 20 382 29 388 1.70e-82 257
MS.gene77959.t1 AT1G02460 41.783 359 181 9 20 357 90 441 1.92e-82 260
MS.gene77959.t1 AT2G43880 40.000 375 215 9 12 383 24 391 1.62e-81 255
MS.gene77959.t1 AT3G15720 41.417 367 192 9 15 372 19 371 1.69e-81 256
MS.gene77959.t1 AT3G59850 41.509 371 203 12 18 382 22 384 2.62e-81 254
MS.gene77959.t1 AT2G43860 43.467 375 199 11 17 385 31 398 2.97e-81 254
MS.gene77959.t1 AT3G59850 41.509 371 203 12 18 382 66 428 6.04e-81 254
MS.gene77959.t1 AT1G80170 47.744 266 134 3 13 274 47 311 2.23e-80 250
MS.gene77959.t1 AT1G80140 40.708 339 186 7 54 385 6 336 1.42e-79 248
MS.gene77959.t1 AT1G48100 37.360 356 202 7 20 360 81 430 1.01e-77 248
MS.gene77959.t1 AT1G10640 39.071 366 193 6 20 357 120 483 2.25e-77 248
MS.gene77959.t1 AT3G26610 38.692 367 205 8 7 361 54 412 2.65e-77 246
MS.gene77959.t1 AT1G60590 36.000 375 207 6 14 357 118 490 2.81e-77 248
MS.gene77959.t1 AT3G15720 43.438 320 163 7 60 372 3 311 3.86e-77 243
MS.gene77959.t1 AT4G01890 37.666 377 209 8 7 363 53 423 9.63e-77 245
MS.gene77959.t1 AT1G65570 37.802 373 221 9 15 382 27 393 2.11e-76 242
MS.gene77959.t1 AT1G02790 37.368 380 211 10 20 384 53 420 2.56e-76 242
MS.gene77959.t1 AT4G32380 38.968 349 198 7 19 359 26 367 7.97e-76 242
MS.gene77959.t1 AT3G07830 38.122 362 212 8 28 383 30 385 1.48e-75 239
MS.gene77959.t1 AT5G48140 37.366 372 220 8 18 383 20 384 2.69e-75 239
MS.gene77959.t1 AT5G44830 39.385 325 178 4 50 372 9 316 6.14e-75 236
MS.gene77959.t1 AT4G35670 47.893 261 128 2 98 358 1 253 7.03e-75 234
MS.gene77959.t1 AT2G33160 39.314 379 216 9 13 382 26 399 1.58e-74 244
MS.gene77959.t1 AT5G17200 38.211 369 194 7 15 359 35 393 9.16e-74 236
MS.gene77959.t1 AT4G13760 37.596 391 222 11 4 383 21 400 2.58e-73 234
MS.gene77959.t1 AT3G07820 37.097 372 222 8 18 383 19 384 4.24e-73 233
MS.gene77959.t1 AT1G17150 38.482 369 216 8 20 382 34 397 2.81e-72 231
MS.gene77959.t1 AT2G40310 37.084 391 224 11 4 383 21 400 1.09e-71 230
MS.gene77959.t1 AT3G07840 36.461 373 223 8 18 383 20 385 3.08e-71 229
MS.gene77959.t1 AT1G43080 36.802 394 227 11 1 383 18 400 1.48e-70 227
MS.gene77959.t1 AT2G15460 37.333 375 218 10 20 383 32 400 9.95e-70 224
MS.gene77959.t1 AT2G15450 37.037 378 220 11 19 384 31 402 4.04e-69 223
MS.gene77959.t1 AT1G43090 37.047 386 221 11 4 378 21 395 5.81e-69 224
MS.gene77959.t1 AT1G78400 36.915 363 218 8 16 372 32 389 6.47e-69 223
MS.gene77959.t1 AT3G07850 39.130 368 216 7 19 382 70 433 1.88e-68 223
MS.gene77959.t1 AT3G14040 38.753 369 218 7 18 382 70 434 4.24e-68 222
MS.gene77959.t1 AT1G43100 36.761 389 224 11 1 378 18 395 9.09e-68 221
MS.gene77959.t1 AT2G15470 36.702 376 221 10 19 383 31 400 2.53e-67 218
MS.gene77959.t1 AT2G26620 36.461 373 222 9 20 383 32 398 6.04e-66 215
MS.gene77959.t1 AT4G32375 40.433 277 156 3 95 369 9 278 1.60e-63 209
MS.gene77959.t1 AT4G32370 37.421 318 179 8 14 319 29 338 7.44e-63 205
MS.gene77959.t1 AT5G14650 40.458 262 151 4 15 274 47 305 5.64e-62 202
MS.gene77959.t1 AT2G43880 40.000 305 174 8 12 313 24 322 8.06e-62 202
MS.gene77959.t1 AT4G32380 35.159 347 173 7 19 359 26 326 6.23e-60 199
MS.gene77959.t1 AT5G39910 30.928 388 190 6 17 383 34 364 2.70e-53 181
MS.gene77959.t1 AT3G42950 27.250 400 245 11 18 382 65 453 3.79e-27 112
MS.gene77959.t1 AT3G62110 29.206 315 184 11 86 372 117 420 4.40e-25 106
MS.gene77959.t1 AT3G62110 29.206 315 184 11 86 372 117 420 4.40e-25 106
MS.gene77959.t1 AT4G23820 26.633 398 248 12 9 373 31 417 1.69e-24 104
MS.gene77959.t1 AT1G19170 25.060 415 257 14 9 383 76 476 1.28e-23 102
MS.gene77959.t1 AT2G23900 26.568 271 172 9 104 357 158 418 4.52e-19 89.0
MS.gene77959.t1 AT3G48950 25.168 298 188 9 86 357 119 407 9.28e-19 88.2
MS.gene77959.t1 AT4G33440 26.974 304 183 10 103 384 173 459 1.58e-18 87.4
MS.gene77959.t1 AT5G41870 24.806 387 242 13 9 359 35 408 1.94e-18 87.0
MS.gene77959.t1 AT5G49215 27.509 269 171 6 107 360 146 405 1.17e-17 84.7
MS.gene77959.t1 AT5G49215 27.509 269 171 6 107 360 148 407 1.50e-17 84.3
MS.gene77959.t1 AT3G06770 27.157 313 195 8 93 386 47 345 8.29e-17 81.6
MS.gene77959.t1 AT3G06770 27.157 313 195 8 93 386 65 363 1.20e-16 81.3
MS.gene77959.t1 AT3G06770 27.157 313 195 8 93 386 65 363 1.20e-16 81.3
MS.gene77959.t1 AT3G16850 24.832 298 194 8 93 373 131 415 1.44e-16 81.3
MS.gene77959.t1 AT4G23500 26.050 238 155 6 104 327 170 400 1.83e-16 81.3
MS.gene77959.t1 AT3G06770 27.157 313 195 8 93 386 135 433 2.34e-16 80.9
MS.gene77959.t1 AT3G06770 27.157 313 195 8 93 386 134 432 2.36e-16 80.9
MS.gene77959.t1 AT3G61490 23.750 320 221 7 77 379 131 444 1.32e-15 78.6
MS.gene77959.t1 AT3G61490 24.534 322 216 8 77 379 229 542 1.39e-15 79.0
MS.gene77959.t1 AT3G61490 23.750 320 221 7 77 379 116 429 1.42e-15 78.6
MS.gene77959.t1 AT3G61490 23.750 320 221 7 77 379 116 429 1.42e-15 78.6
MS.gene77959.t1 AT3G61490 23.750 320 221 7 77 379 116 429 1.42e-15 78.6

Find 78 sgRNAs with CRISPR-Local

Find 187 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAACCCAATACTGCATTAA+AGG 0.186184 3.2:+21141943 MS.gene77959:CDS
GTTTCATTATGTTAGATCTA+TGG 0.262928 3.2:+21142050 MS.gene77959:intron
ATCTAAATGCAGGGAGGTTC+TGG 0.263116 3.2:+21144567 MS.gene77959:intron
CATGGCCCTGAAAATGTTAT+AGG 0.267346 3.2:-21141920 None:intergenic
GCTCCATATGCTGAAATATT+AGG 0.274821 3.2:-21142089 None:intergenic
TTGAAGGATGCAACAATGTT+TGG 0.280649 3.2:+21142934 MS.gene77959:CDS
TTTACCTTTAATGCAGTATT+GGG 0.302256 3.2:-21141947 None:intergenic
GTGATGTACCAAAACATAAA+AGG 0.305023 3.2:+21145143 MS.gene77959:CDS
AACTTGTCCATCATTAGATT+TGG 0.311900 3.2:-21141492 None:intergenic
ATGCACAACAAATGCATATA+AGG 0.313150 3.2:+21142910 MS.gene77959:CDS
GATGGTGGTGGTACCATTAA+TGG 0.331489 3.2:+21142161 MS.gene77959:CDS
AATATTAGGCCATGCTTTGA+TGG 0.334356 3.2:-21142075 None:intergenic
GTGAACAGAGCCACACTAAT+AGG 0.355997 3.2:+21143846 MS.gene77959:CDS
CTTTATTCTGACTCCGTTAG+TGG 0.375973 3.2:-21143871 None:intergenic
ATTTACCTTTAATGCAGTAT+TGG 0.379250 3.2:-21141948 None:intergenic
ATAATTAACATTGTCACATT+TGG 0.385288 3.2:-21145279 None:intergenic
AGATGATAGACACTATGTTT+AGG 0.399830 3.2:-21141893 None:intergenic
TGCGGCGAAGGCGCGATAGA+TGG 0.401102 3.2:-21141434 None:intergenic
TGAAGGATGCAACAATGTTT+GGG 0.406265 3.2:+21142935 MS.gene77959:CDS
ACGGTTAACGTTGACGACTA+TGG 0.411063 3.2:+21141467 MS.gene77959:CDS
TGGCCTAATATTTCAGCATA+TGG 0.414557 3.2:+21142086 MS.gene77959:CDS
AATGTTAATTATTCAAATAG+TGG 0.428526 3.2:+21145290 MS.gene77959:CDS
GTGGACCAGGACATGGAATC+AGG 0.439750 3.2:+21143637 MS.gene77959:CDS
TTATGAAAACATTCTCTGAA+TGG 0.443046 3.2:-21143019 None:intergenic
GGTGGTACCATTAATGGAAA+TGG 0.446333 3.2:+21142167 MS.gene77959:CDS
TATGAAAACATTCTCTGAAT+GGG 0.447211 3.2:-21143018 None:intergenic
TTTGTTCATGGGTAACACTT+AGG 0.447678 3.2:-21145323 None:intergenic
TTCATAACCAACAGCACCAT+TGG 0.449118 3.2:+21143035 MS.gene77959:CDS
CAAATGCATATAAGGATTGA+AGG 0.456482 3.2:+21142918 MS.gene77959:CDS
CCTTTAATGCAGTATTGGGT+TGG 0.462412 3.2:-21141943 None:intergenic
TGGAATGAGGCATGTTCTAA+AGG 0.468725 3.2:+21141854 MS.gene77959:CDS
TTTAGGCATTTGAAATGTCA+TGG 0.469564 3.2:+21141834 MS.gene77959:intron
ACCTGTCCCAATGGTGCTGT+TGG 0.469575 3.2:-21143042 None:intergenic
AACAATTTAGTTTCATGGCA+AGG 0.470742 3.2:+21141402 None:intergenic
GTCACAGATCTCATAGTTGA+TGG 0.474884 3.2:+21142143 MS.gene77959:CDS
ATGGAGCAGATAGAAAGCTT+TGG 0.483983 3.2:+21142105 MS.gene77959:CDS
TCAGTGTTGGGACTGTGTCC+TGG 0.491323 3.2:-21142981 None:intergenic
GGAATGAGGCATGTTCTAAA+GGG 0.495884 3.2:+21141855 MS.gene77959:CDS
TCATAACCAACAGCACCATT+GGG 0.496240 3.2:+21143036 MS.gene77959:CDS
TGCAAGTGAACTCCTTTGCA+TGG 0.498082 3.2:-21145215 None:intergenic
ATATGAATTCCATCAGTGTT+GGG 0.505639 3.2:-21142993 None:intergenic
ACAATTTAGTTTCATGGCAA+GGG 0.511066 3.2:+21141403 None:intergenic
AACATTGTCACATTTGGCAG+TGG 0.514203 3.2:-21145273 None:intergenic
AATATGAATTCCATCAGTGT+TGG 0.516408 3.2:-21142994 None:intergenic
TGTAGCATTGGAAGCTTAGG+AGG 0.519121 3.2:+21143789 MS.gene77959:intron
ATTGTATTTCAATTGTAAGT+GGG 0.522080 3.2:+21143583 MS.gene77959:CDS
TGAATGCAATAATTTGAAAG+TGG 0.522545 3.2:+21142869 MS.gene77959:CDS
ATGAACCTGATTCCATGTCC+TGG 0.522587 3.2:-21143642 None:intergenic
AATGCAGTATTGGGTTGGCA+TGG 0.531497 3.2:-21141938 None:intergenic
GATTGTATTTCAATTGTAAG+TGG 0.534722 3.2:+21143582 MS.gene77959:CDS
ATTTGAAATGTCATGGAATG+AGG 0.544227 3.2:+21141841 MS.gene77959:CDS
ACCAACAGCACCATTGGGAC+AGG 0.545836 3.2:+21143041 MS.gene77959:CDS
TTTATTTCACAGCCATGCAA+GGG 0.548985 3.2:+21142721 MS.gene77959:intron
TATGGAGCCAAATCTAATGA+TGG 0.549828 3.2:+21141485 MS.gene77959:CDS
TTCTAAAGGGAAGGTTCTTG+TGG 0.553586 3.2:+21141868 MS.gene77959:CDS
GCAAGTGAACTCCTTTGCAT+GGG 0.556459 3.2:-21145214 None:intergenic
TGATGGACAAGTTGATAATG+AGG 0.564129 3.2:+21141502 MS.gene77959:CDS
CGAGCAACAGATATTACTTG+TGG 0.574897 3.2:+21143618 MS.gene77959:CDS
AGTCAGAATAAAGACTTGGC+AGG 0.577736 3.2:+21143881 MS.gene77959:CDS
AATCTAATAGTCACAGCACC+AGG 0.584074 3.2:+21142963 MS.gene77959:CDS
GGACACAGTCCCAACACTGA+TGG 0.584378 3.2:+21142984 MS.gene77959:CDS
ATTACTTGTGGACCAGGACA+TGG 0.589105 3.2:+21143630 MS.gene77959:CDS
GATCTCATAGTTGATGGTGG+TGG 0.596400 3.2:+21142149 MS.gene77959:CDS
AGCAAAGCTGTCCCATGCAA+AGG 0.601502 3.2:+21145203 MS.gene77959:CDS
GTTAGTGGTTCCTATTAGTG+TGG 0.606907 3.2:-21143856 None:intergenic
ACAGATATTACTTGTGGACC+AGG 0.610780 3.2:+21143624 MS.gene77959:CDS
AGCATTGGAAGCTTAGGAGG+AGG 0.616219 3.2:+21143792 MS.gene77959:intron
TGAGGCATGTTCTAAAGGGA+AGG 0.623152 3.2:+21141859 MS.gene77959:CDS
ACGGAGTCAGAATAAAGACT+TGG 0.631544 3.2:+21143877 MS.gene77959:CDS
AGAAGCAGAAGTGTCTAATG+TGG 0.639272 3.2:+21143821 MS.gene77959:CDS
ACACTAATAGGAACCACTAA+CGG 0.642670 3.2:+21143858 MS.gene77959:CDS
CAAATTGCAGAACTCAGCTG+TGG 0.648005 3.2:+21145109 MS.gene77959:intron
TTATTTCACAGCCATGCAAG+GGG 0.654101 3.2:+21142722 MS.gene77959:intron
TCTATGGAACCATCAAAGCA+TGG 0.658530 3.2:+21142066 MS.gene77959:CDS
GATGTGAAATTAACAGCACA+AGG 0.676145 3.2:+21145239 MS.gene77959:CDS
ACAGATCTCATAGTTGATGG+TGG 0.709690 3.2:+21142146 MS.gene77959:CDS
TTCGCCGCAATCTCAAAACA+CGG 0.711068 3.2:+21141448 MS.gene77959:CDS
TATTTCACAGCCATGCAAGG+GGG 0.733319 3.2:+21142723 MS.gene77959:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTATAAAGTAAAAAAAG+TGG + chr3.2:21143954-21143973 MS.gene77959:intron 10.0%
!! ATTGATTAAAAAATATGATA+TGG + chr3.2:21143712-21143731 MS.gene77959:intron 10.0%
!!! ATGGTTTATTTTATAATTTT+GGG + chr3.2:21144046-21144065 MS.gene77959:intron 10.0%
!!! TTATTCAATTTAATTTTTCA+TGG + chr3.2:21142812-21142831 MS.gene77959:intron 10.0%
!! AATGTTAATTATTCAAATAG+TGG + chr3.2:21145290-21145309 MS.gene77959:CDS 15.0%
!! AGGTAAATTAAAATGATTTA+TGG + chr3.2:21141963-21141982 MS.gene77959:intron 15.0%
!! ATTAAAATGAATGAAAACAT+GGG - chr3.2:21144945-21144964 None:intergenic 15.0%
!! GATAAAAAAAAATTCCAATT+AGG - chr3.2:21143674-21143693 None:intergenic 15.0%
!! GATTAAATGAATGAATTAAA+AGG - chr3.2:21142256-21142275 None:intergenic 15.0%
!! GCAAATAAACTAATAATAAA+TGG - chr3.2:21142597-21142616 None:intergenic 15.0%
!! TATTAAAATGAATGAAAACA+TGG - chr3.2:21144946-21144965 None:intergenic 15.0%
!! TGAATCAAATTAAAATGATA+AGG - chr3.2:21142665-21142684 None:intergenic 15.0%
!! TTAAAATATTTATGTGATCA+AGG + chr3.2:21141670-21141689 MS.gene77959:intron 15.0%
!!! AATTAGTGATTTGAATTTTA+TGG + chr3.2:21142617-21142636 MS.gene77959:intron 15.0%
!!! AATTAGTTATTTTCTTTACT+TGG - chr3.2:21142449-21142468 None:intergenic 15.0%
!!! ACACTCATTTATAAATTTTA+AGG + chr3.2:21143922-21143941 MS.gene77959:intron 15.0%
!!! ATATTTTTGAGTTATGATTA+TGG - chr3.2:21144646-21144665 None:intergenic 15.0%
!!! ATGAGTTTTATATATTTAAG+TGG - chr3.2:21142005-21142024 None:intergenic 15.0%
!!! ATTTTGGATTTGTTAAATTA+AGG + chr3.2:21142285-21142304 MS.gene77959:intron 15.0%
!!! CATGGTTTATTTTATAATTT+TGG + chr3.2:21144045-21144064 MS.gene77959:intron 15.0%
!!! TATTTTTGAGTTATGATTAT+GGG - chr3.2:21144645-21144664 None:intergenic 15.0%
!! AATGAAAAAAAACTCTTTGT+TGG - chr3.2:21144352-21144371 None:intergenic 20.0%
!! ATAATTAACATTGTCACATT+TGG - chr3.2:21145282-21145301 None:intergenic 20.0%
!! ATACTATATATCTAAATGCA+GGG + chr3.2:21144558-21144577 MS.gene77959:intron 20.0%
!! ATACTTTAACTAACTAAGTT+TGG + chr3.2:21144217-21144236 MS.gene77959:intron 20.0%
!! ATCAATCAATTCAAAATTCA+AGG - chr3.2:21143698-21143717 None:intergenic 20.0%
!! ATTATAGAAATATTGAGGAA+AGG - chr3.2:21142477-21142496 None:intergenic 20.0%
!! ATTGGTTAAAAAATTTGATG+TGG + chr3.2:21143754-21143773 MS.gene77959:intron 20.0%
!! ATTGTATTTCAATTGTAAGT+GGG + chr3.2:21143583-21143602 MS.gene77959:CDS 20.0%
!! GAAACTAAACAAATAAAACA+AGG - chr3.2:21144978-21144997 None:intergenic 20.0%
!! GATATTTGTTTCCATTTAAA+TGG + chr3.2:21141785-21141804 MS.gene77959:intron 20.0%
!! GTTTAAATTAGTTAAGAAGA+AGG - chr3.2:21144330-21144349 None:intergenic 20.0%
!! TACTAATGTAATCAAAATCA+TGG + chr3.2:21145085-21145104 MS.gene77959:intron 20.0%
!! TTAATGGAAATGGAAAAATA+TGG + chr3.2:21142177-21142196 MS.gene77959:CDS 20.0%
!! TTTACCAAATTATTATCACA+AGG + chr3.2:21144010-21144029 MS.gene77959:intron 20.0%
!! TTTCTAACTAAACATAAAGT+TGG + chr3.2:21144187-21144206 MS.gene77959:intron 20.0%
!!! ATGAAAATTTAACTTACTGT+TGG - chr3.2:21142751-21142770 None:intergenic 20.0%
!!! ATTTGAGTTAATATCATACA+AGG - chr3.2:21142232-21142251 None:intergenic 20.0%
!!! GGTAAATACTTGTTAATTTA+GGG + chr3.2:21144067-21144086 MS.gene77959:intron 20.0%
!!! GTTTTATTTGTTTAGTTTCA+TGG + chr3.2:21144979-21144998 MS.gene77959:intron 20.0%
!!! TAATTATTGTTTTGTAGCAT+TGG + chr3.2:21143777-21143796 MS.gene77959:intron 20.0%
!!! TATTTTTCCATTTCCATTAA+TGG - chr3.2:21142177-21142196 None:intergenic 20.0%
!!! TCCTCAATATTTCTATAATT+AGG + chr3.2:21142478-21142497 MS.gene77959:intron 20.0%
!!! TTAAGACAAAAAGTCATTTT+AGG + chr3.2:21143447-21143466 MS.gene77959:intron 20.0%
!!! TTTTGAAAGATAAACGTTTT+TGG + chr3.2:21144517-21144536 MS.gene77959:intron 20.0%
! AAACAAATAAAACAAGGCAA+GGG - chr3.2:21144972-21144991 None:intergenic 25.0%
! AAAGGAGAGTAAAGAAATTA+TGG - chr3.2:21142703-21142722 None:intergenic 25.0%
! AAATTAACGAACAAAATCAG+AGG + chr3.2:21144759-21144778 MS.gene77959:intron 25.0%
! AATAAACCATGTTTACTTGA+AGG - chr3.2:21144036-21144055 None:intergenic 25.0%
! ATATATTTAAGTGGACATTG+AGG - chr3.2:21141996-21142015 None:intergenic 25.0%
! ATTATCAATCCATGTAACAA+TGG - chr3.2:21144133-21144152 None:intergenic 25.0%
! ATTTACCTTTAATGCAGTAT+TGG - chr3.2:21141951-21141970 None:intergenic 25.0%
! ATTTGAGAACACCATTTAAA+TGG - chr3.2:21141799-21141818 None:intergenic 25.0%
! CATACTATATATCTAAATGC+AGG + chr3.2:21144557-21144576 MS.gene77959:intron 25.0%
! GATTGTATTTCAATTGTAAG+TGG + chr3.2:21143582-21143601 MS.gene77959:CDS 25.0%
! GCCTAATTATAGAAATATTG+AGG - chr3.2:21142482-21142501 None:intergenic 25.0%
! GTTTCATTATGTTAGATCTA+TGG + chr3.2:21142050-21142069 MS.gene77959:intron 25.0%
! TAAACAAATAAAACAAGGCA+AGG - chr3.2:21144973-21144992 None:intergenic 25.0%
! TATATTTAAGTGGACATTGA+GGG - chr3.2:21141995-21142014 None:intergenic 25.0%
! TATGAAAACATTCTCTGAAT+GGG - chr3.2:21143021-21143040 None:intergenic 25.0%
! TGAATGCAATAATTTGAAAG+TGG + chr3.2:21142869-21142888 MS.gene77959:CDS 25.0%
! TGCATTAGCACTATTATATT+GGG + chr3.2:21144100-21144119 MS.gene77959:intron 25.0%
! TTATGAAAACATTCTCTGAA+TGG - chr3.2:21143022-21143041 None:intergenic 25.0%
! TTGCATTAGCACTATTATAT+TGG + chr3.2:21144099-21144118 MS.gene77959:intron 25.0%
! TTGTAAAATCGTGTATTTGT+TGG + chr3.2:21141739-21141758 MS.gene77959:intron 25.0%
! TTTACCTTTAATGCAGTATT+GGG - chr3.2:21141950-21141969 None:intergenic 25.0%
!! ATTGTTACATGGATTGATAA+TGG + chr3.2:21144132-21144151 MS.gene77959:intron 25.0%
!! CAAATACACGATTTTACAAT+CGG - chr3.2:21141738-21141757 None:intergenic 25.0%
!! CAATATTTCTATAATTAGGC+TGG + chr3.2:21142482-21142501 MS.gene77959:intron 25.0%
!! GGGTAAATACTTGTTAATTT+AGG + chr3.2:21144066-21144085 MS.gene77959:intron 25.0%
!! TAAAGCCTATAACATTTTCA+GGG + chr3.2:21141915-21141934 MS.gene77959:CDS 25.0%
!! TTGACATGTTTGAAGTAATA+AGG - chr3.2:21144271-21144290 None:intergenic 25.0%
!!! ATTTCTCATTTTTGCACTTT+AGG + chr3.2:21141817-21141836 MS.gene77959:intron 25.0%
!!! GGAAAATTTGTTGCATATAT+AGG + chr3.2:21142833-21142852 MS.gene77959:intron 25.0%
AAAAAAGAAATGTCAAGAGC+AGG + chr3.2:21144674-21144693 MS.gene77959:CDS 30.0%
AACAAATAAAACAAGGCAAG+GGG - chr3.2:21144971-21144990 None:intergenic 30.0%
AACTTGTCCATCATTAGATT+TGG - chr3.2:21141495-21141514 None:intergenic 30.0%
AATATGAATTCCATCAGTGT+TGG - chr3.2:21142997-21143016 None:intergenic 30.0%
AATTTAGCGAAAACAATGTG+TGG + chr3.2:21141526-21141545 MS.gene77959:intron 30.0%
ACAAATCCAAAATCAATGCA+CGG - chr3.2:21142278-21142297 None:intergenic 30.0%
AGATGATAGACACTATGTTT+AGG - chr3.2:21141896-21141915 None:intergenic 30.0%
ATATGAATTCCATCAGTGTT+GGG - chr3.2:21142996-21143015 None:intergenic 30.0%
ATCACCAATCACATTATGAT+TGG + chr3.2:21141711-21141730 MS.gene77959:intron 30.0%
ATGCACAACAAATGCATATA+AGG + chr3.2:21142910-21142929 MS.gene77959:CDS 30.0%
ATGGAAATGGAAAAATATGG+TGG + chr3.2:21142180-21142199 MS.gene77959:CDS 30.0%
CAAATGCATATAAGGATTGA+AGG + chr3.2:21142918-21142937 MS.gene77959:CDS 30.0%
GAAGTAATAAGGTTAGAAAC+TGG - chr3.2:21144260-21144279 None:intergenic 30.0%
GTGATGTACCAAAACATAAA+AGG + chr3.2:21145143-21145162 MS.gene77959:CDS 30.0%
TAGACCTAAAACTCAAGTTT+AGG - chr3.2:21144791-21144810 None:intergenic 30.0%
TAGTGCTAATGCAACTAATT+TGG - chr3.2:21144093-21144112 None:intergenic 30.0%
TGGAAATGGAAAAATATGGT+GGG + chr3.2:21142181-21142200 MS.gene77959:CDS 30.0%
TTGAATGTCACTCTACAAAA+CGG - chr3.2:21141644-21141663 None:intergenic 30.0%
! AAGGCCTTGTGATAATAATT+TGG - chr3.2:21144017-21144036 None:intergenic 30.0%
! ACAACCTAAACTTGAGTTTT+AGG + chr3.2:21144784-21144803 MS.gene77959:intron 30.0%
! ATCATAATGTGATTGGTGAT+CGG - chr3.2:21141711-21141730 None:intergenic 30.0%
! ATTTGAAATGTCATGGAATG+AGG + chr3.2:21141841-21141860 MS.gene77959:CDS 30.0%
! CACTTGTTCCTTTTATGTTT+TGG - chr3.2:21145154-21145173 None:intergenic 30.0%
! CTAAAGCCTATAACATTTTC+AGG + chr3.2:21141914-21141933 MS.gene77959:CDS 30.0%
! TTTAGGCATTTGAAATGTCA+TGG + chr3.2:21141834-21141853 MS.gene77959:intron 30.0%
!! ATTCCTCACTATTTCTGTTA+AGG + chr3.2:21143555-21143574 MS.gene77959:intron 30.0%
!!! GCTCTTGACATTTCTTTTTT+TGG - chr3.2:21144674-21144693 None:intergenic 30.0%
!!! TTTAATCCGTGCATTGATTT+TGG + chr3.2:21142269-21142288 MS.gene77959:intron 30.0%
AATATTAGGCCATGCTTTGA+TGG - chr3.2:21142078-21142097 None:intergenic 35.0%
AATGAATGAAAACATGGGCT+TGG - chr3.2:21144940-21144959 None:intergenic 35.0%
ACAAATAAAACAAGGCAAGG+GGG - chr3.2:21144970-21144989 None:intergenic 35.0%
ACACTAATAGGAACCACTAA+CGG + chr3.2:21143858-21143877 MS.gene77959:CDS 35.0%
CTATATATCTAAATGCAGGG+AGG + chr3.2:21144561-21144580 MS.gene77959:intron 35.0%
GATGTGAAATTAACAGCACA+AGG + chr3.2:21145239-21145258 MS.gene77959:CDS 35.0%
GCTCCATATGCTGAAATATT+AGG - chr3.2:21142092-21142111 None:intergenic 35.0%
TATAGAGCAACACATGATGT+GGG + chr3.2:21144833-21144852 MS.gene77959:intron 35.0%
TATGGAGCCAAATCTAATGA+TGG + chr3.2:21141485-21141504 MS.gene77959:CDS 35.0%
TCACCTTAACAGAAATAGTG+AGG - chr3.2:21143561-21143580 None:intergenic 35.0%
TGAAGGATGCAACAATGTTT+GGG + chr3.2:21142935-21142954 MS.gene77959:CDS 35.0%
TGGCCTAATATTTCAGCATA+TGG + chr3.2:21142086-21142105 MS.gene77959:CDS 35.0%
TTCAGAGCAACACAAGATAT+GGG + chr3.2:21144858-21144877 MS.gene77959:intron 35.0%
TTGAAGGATGCAACAATGTT+TGG + chr3.2:21142934-21142953 MS.gene77959:CDS 35.0%
TTGCATGGCTGTGAAATAAA+AGG - chr3.2:21142721-21142740 None:intergenic 35.0%
TTTATTTCACAGCCATGCAA+GGG + chr3.2:21142721-21142740 MS.gene77959:intron 35.0%
TTTCAGAGCAACACAAGATA+TGG + chr3.2:21144857-21144876 MS.gene77959:intron 35.0%
! ATCTGTTGCTCGAACATTTT+TGG - chr3.2:21143610-21143629 None:intergenic 35.0%
! TATCTTTTTACCTGTCCCAA+TGG - chr3.2:21143054-21143073 None:intergenic 35.0%
!! TGATGGACAAGTTGATAATG+AGG + chr3.2:21141502-21141521 MS.gene77959:CDS 35.0%
!! TTTTATTTCACAGCCATGCA+AGG + chr3.2:21142720-21142739 MS.gene77959:intron 35.0%
!! TTTTGTAGCATTGGAAGCTT+AGG + chr3.2:21143786-21143805 MS.gene77959:intron 35.0%
!!! TTAACCGTGTTTTGAGATTG+CGG - chr3.2:21141455-21141474 None:intergenic 35.0%
AACATTGTCACATTTGGCAG+TGG - chr3.2:21145276-21145295 None:intergenic 40.0%
AATCTAATAGTCACAGCACC+AGG + chr3.2:21142963-21142982 MS.gene77959:CDS 40.0%
ACAAGGCCTTCAAGTAAACA+TGG + chr3.2:21144027-21144046 MS.gene77959:intron 40.0%
ACAGATATTACTTGTGGACC+AGG + chr3.2:21143624-21143643 MS.gene77959:CDS 40.0%
ACGGAGTCAGAATAAAGACT+TGG + chr3.2:21143877-21143896 MS.gene77959:CDS 40.0%
AGGTTCATGACTCTCCTAAT+TGG + chr3.2:21143657-21143676 MS.gene77959:intron 40.0%
AGTCAGAATAAAGACTTGGC+AGG + chr3.2:21143881-21143900 MS.gene77959:CDS 40.0%
ATAGAGCAACACATGATGTG+GGG + chr3.2:21144834-21144853 MS.gene77959:intron 40.0%
ATGGAGCAGATAGAAAGCTT+TGG + chr3.2:21142105-21142124 MS.gene77959:CDS 40.0%
CAAATAAAACAAGGCAAGGG+GGG - chr3.2:21144969-21144988 None:intergenic 40.0%
CATGGCCCTGAAAATGTTAT+AGG - chr3.2:21141923-21141942 None:intergenic 40.0%
CCAACCCAATACTGCATTAA+AGG + chr3.2:21141943-21141962 MS.gene77959:CDS 40.0%
CGAGCAACAGATATTACTTG+TGG + chr3.2:21143618-21143637 MS.gene77959:CDS 40.0%
CGGACCAATCATAATGTGAT+TGG - chr3.2:21141718-21141737 None:intergenic 40.0%
CTTTATTCTGACTCCGTTAG+TGG - chr3.2:21143874-21143893 None:intergenic 40.0%
GGAAGTTGACCATTGTTACA+TGG + chr3.2:21144121-21144140 MS.gene77959:intron 40.0%
GGTGGTACCATTAATGGAAA+TGG + chr3.2:21142167-21142186 MS.gene77959:CDS 40.0%
GTATAGAGCAACACATGATG+TGG + chr3.2:21144832-21144851 MS.gene77959:intron 40.0%
GTCACAGATCTCATAGTTGA+TGG + chr3.2:21142143-21142162 MS.gene77959:CDS 40.0%
GTTAGTGGTTCCTATTAGTG+TGG - chr3.2:21143859-21143878 None:intergenic 40.0%
TCATAACCAACAGCACCATT+GGG + chr3.2:21143036-21143055 MS.gene77959:CDS 40.0%
TCTATGGAACCATCAAAGCA+TGG + chr3.2:21142066-21142085 MS.gene77959:CDS 40.0%
TGGAATGAGGCATGTTCTAA+AGG + chr3.2:21141854-21141873 MS.gene77959:CDS 40.0%
TTATTTCACAGCCATGCAAG+GGG + chr3.2:21142722-21142741 MS.gene77959:intron 40.0%
TTCATAACCAACAGCACCAT+TGG + chr3.2:21143035-21143054 MS.gene77959:CDS 40.0%
! CCTTTAATGCAGTATTGGGT+TGG - chr3.2:21141946-21141965 None:intergenic 40.0%
! GGAATGAGGCATGTTCTAAA+GGG + chr3.2:21141855-21141874 MS.gene77959:CDS 40.0%
! TTCTAAAGGGAAGGTTCTTG+TGG + chr3.2:21141868-21141887 MS.gene77959:CDS 40.0%
!! ACAGATCTCATAGTTGATGG+TGG + chr3.2:21142146-21142165 MS.gene77959:CDS 40.0%
!! AGAAGCAGAAGTGTCTAATG+TGG + chr3.2:21143821-21143840 MS.gene77959:CDS 40.0%
ACGGTTAACGTTGACGACTA+TGG + chr3.2:21141467-21141486 MS.gene77959:CDS 45.0%
ATCTAAATGCAGGGAGGTTC+TGG + chr3.2:21144567-21144586 MS.gene77959:intron 45.0%
ATGAACCTGATTCCATGTCC+TGG - chr3.2:21143645-21143664 None:intergenic 45.0%
ATTACTTGTGGACCAGGACA+TGG + chr3.2:21143630-21143649 MS.gene77959:CDS 45.0%
CAAATTGCAGAACTCAGCTG+TGG + chr3.2:21145109-21145128 MS.gene77959:intron 45.0%
GCAAGTGAACTCCTTTGCAT+GGG - chr3.2:21145217-21145236 None:intergenic 45.0%
GTGAACAGAGCCACACTAAT+AGG + chr3.2:21143846-21143865 MS.gene77959:CDS 45.0%
TATTTCACAGCCATGCAAGG+GGG + chr3.2:21142723-21142742 MS.gene77959:intron 45.0%
TGCAAGTGAACTCCTTTGCA+TGG - chr3.2:21145218-21145237 None:intergenic 45.0%
TTCGCCGCAATCTCAAAACA+CGG + chr3.2:21141448-21141467 MS.gene77959:CDS 45.0%
! TGTAGCATTGGAAGCTTAGG+AGG + chr3.2:21143789-21143808 MS.gene77959:intron 45.0%
! TGTGATTGGTGATCGGAACA+AGG - chr3.2:21141704-21141723 None:intergenic 45.0%
!! AATGCAGTATTGGGTTGGCA+TGG - chr3.2:21141941-21141960 None:intergenic 45.0%
!! GATCTCATAGTTGATGGTGG+TGG + chr3.2:21142149-21142168 MS.gene77959:CDS 45.0%
!! GATGGTGGTGGTACCATTAA+TGG + chr3.2:21142161-21142180 MS.gene77959:CDS 45.0%
!! TGAGGCATGTTCTAAAGGGA+AGG + chr3.2:21141859-21141878 MS.gene77959:CDS 45.0%
!! AAATTTATTTACAAATTAAA+AGG - chr3.2:21142508-21142527 None:intergenic 5.0%
!! AACTATTTATTATTATTTAT+TGG - chr3.2:21143509-21143528 None:intergenic 5.0%
!! AATAAAAAATTAAATTAAGT+CGG - chr3.2:21143991-21144010 None:intergenic 5.0%
!! ACTATTTATTATTATTTATT+GGG - chr3.2:21143508-21143527 None:intergenic 5.0%
!!! AATTAATTTTTTTAATTGAT+TGG + chr3.2:21143736-21143755 MS.gene77959:intron 5.0%
!!! GATTTTTTTTTTTATAATAA+TGG + chr3.2:21144154-21144173 MS.gene77959:intron 5.0%
AGCAAAGCTGTCCCATGCAA+AGG + chr3.2:21145203-21145222 MS.gene77959:CDS 50.0%
! AGCATTGGAAGCTTAGGAGG+AGG + chr3.2:21143792-21143811 MS.gene77959:intron 50.0%
!!! GTGTTTTGAGATTGCGGCGA+AGG - chr3.2:21141449-21141468 None:intergenic 50.0%
ACCAACAGCACCATTGGGAC+AGG + chr3.2:21143041-21143060 MS.gene77959:CDS 55.0%
GGACACAGTCCCAACACTGA+TGG + chr3.2:21142984-21143003 MS.gene77959:CDS 55.0%
GTGGACCAGGACATGGAATC+AGG + chr3.2:21143637-21143656 MS.gene77959:CDS 55.0%
! TCAGTGTTGGGACTGTGTCC+TGG - chr3.2:21142984-21143003 None:intergenic 55.0%
!! ACCTGTCCCAATGGTGCTGT+TGG - chr3.2:21143045-21143064 None:intergenic 55.0%
! ACTGTTGGTGCCCCCTTGCA+TGG - chr3.2:21142736-21142755 None:intergenic 60.0%
! TGCGGCGAAGGCGCGATAGA+TGG - chr3.2:21141437-21141456 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 21141416 21145340 21141416 ID=MS.gene77959
chr3.2 mRNA 21141416 21145340 21141416 ID=MS.gene77959.t1;Parent=MS.gene77959
chr3.2 exon 21141416 21141523 21141416 ID=MS.gene77959.t1.exon1;Parent=MS.gene77959.t1
chr3.2 CDS 21141416 21141523 21141416 ID=cds.MS.gene77959.t1;Parent=MS.gene77959.t1
chr3.2 exon 21141839 21141964 21141839 ID=MS.gene77959.t1.exon2;Parent=MS.gene77959.t1
chr3.2 CDS 21141839 21141964 21141839 ID=cds.MS.gene77959.t1;Parent=MS.gene77959.t1
chr3.2 exon 21142065 21142232 21142065 ID=MS.gene77959.t1.exon3;Parent=MS.gene77959.t1
chr3.2 CDS 21142065 21142232 21142065 ID=cds.MS.gene77959.t1;Parent=MS.gene77959.t1
chr3.2 exon 21142733 21142753 21142733 ID=MS.gene77959.t1.exon4;Parent=MS.gene77959.t1
chr3.2 CDS 21142733 21142753 21142733 ID=cds.MS.gene77959.t1;Parent=MS.gene77959.t1
chr3.2 exon 21142855 21143062 21142855 ID=MS.gene77959.t1.exon5;Parent=MS.gene77959.t1
chr3.2 CDS 21142855 21143062 21142855 ID=cds.MS.gene77959.t1;Parent=MS.gene77959.t1
chr3.2 exon 21143577 21143658 21143577 ID=MS.gene77959.t1.exon6;Parent=MS.gene77959.t1
chr3.2 CDS 21143577 21143658 21143577 ID=cds.MS.gene77959.t1;Parent=MS.gene77959.t1
chr3.2 exon 21143794 21143902 21143794 ID=MS.gene77959.t1.exon7;Parent=MS.gene77959.t1
chr3.2 CDS 21143794 21143902 21143794 ID=cds.MS.gene77959.t1;Parent=MS.gene77959.t1
chr3.2 exon 21144579 21144695 21144579 ID=MS.gene77959.t1.exon8;Parent=MS.gene77959.t1
chr3.2 CDS 21144579 21144695 21144579 ID=cds.MS.gene77959.t1;Parent=MS.gene77959.t1
chr3.2 exon 21145119 21145340 21145119 ID=MS.gene77959.t1.exon9;Parent=MS.gene77959.t1
chr3.2 CDS 21145119 21145340 21145119 ID=cds.MS.gene77959.t1;Parent=MS.gene77959.t1
Gene Sequence

>MS.gene77959

ATGGCAAGGGCTCATCATCCATCTATCGCGCCTTCGCCGCAATCTCAAAACACGGTTAACGTTGACGACTATGGAGCCAAATCTAATGATGGACAAGTTGATAATGAGGTAATTTAGCGAAAACAATGTGTGGTATATTTTTACTGAAGTTGTATTTGAATCTCACGTTGTCATGATTCATGAGTTATGCAATGCTTATATGTGTTTTTGTCTCATCTTACAAGTCCGTTTTGTAGAGTGACATTCAAATTCATTTAAAATATTTATGTGATCAAGGTTTAACTGCCTTGTTCCGATCACCAATCACATTATGATTGGTCCGATTGTAAAATCGTGTATTTGTTGGTCCGATTGTAAAATCGTGTATTTGATATTTGTTTCCATTTAAATGGTGTTCTCAAATTTCTCATTTTTGCACTTTAGGCATTTGAAATGTCATGGAATGAGGCATGTTCTAAAGGGAAGGTTCTTGTGGTACCTAAACATAGTGTCTATCATCTAAAGCCTATAACATTTTCAGGGCCATGCCAACCCAATACTGCATTAAAGGTAAATTAAAATGATTTATGGTTATAACCCTCAATGTCCACTTAAATATATAAAACTCATTGTAACGTTTTTGTGTTTAATTACTGTTTCATTATGTTAGATCTATGGAACCATCAAAGCATGGCCTAATATTTCAGCATATGGAGCAGATAGAAAGCTTTGGATCAAGTTTGAGAATGTCACAGATCTCATAGTTGATGGTGGTGGTACCATTAATGGAAATGGAAAAATATGGTGGGAGAATTCTTGCAAAGTGAATGAAAGCCTTGTATGATATTAACTCAAATTCCTTTTAATTCATTCATTTAATCCGTGCATTGATTTTGGATTTGTTAAATTAAGGAAAAATATATTTTGTCATTTTTTGTTTCTTGTTTACTTTTTAGTATTAACTAAAAATCGTATACTATTATACACTTTAATTTTTTTCTTTTCAAAAATTTATTTTGTTTATACAAACATAAAGAAAATAACTTCTTTGCCAAGTAAAGAAAATAACTAATTATTCACCTTTCCTCAATATTTCTATAATTAGGCTGGCCTTTTAATTTGTAAATAAATTTTTTTTACAAATAAAGAAAATAACTTACAAAGAAACATAAAGAATTGTTTTTAAGAAATTTTAATAACCATTTATTATTAGTTTATTTGCAATTAGTGATTTGAATTTTATGGAATCATTATATAGACTTTTTTTCCTTATCATTTTAATTTGATTCAAATTTTAAATACTGACCATAATTTCTTTACTCTCCTTTTATTTCACAGCCATGCAAGGGGGCACCAACAGTAAGTTAAATTTTCATTTTTGTGAACTTTATTACATGCTTTTCTGAAATACTCATCTTTATTCAATTTAATTTTTCATGGAAAATTTGTTGCATATATAGGCTGTGACTTTCGATGAATGCAATAATTTGAAAGTGGATAAGATAAGATTCAGAAATGCACAACAAATGCATATAAGGATTGAAGGATGCAACAATGTTTGGGCATCAAATCTAATAGTCACAGCACCAGGACACAGTCCCAACACTGATGGAATTCATATTACCCATTCAGAGAATGTTTTCATAACCAACAGCACCATTGGGACAGGTAAAAAGATATATAAATGAAAAAAAATGTCACATTATAATATCATTTTTCATAATATGTGTTTTCATATTATAATATCATTTTTTTTAAATCATAACTAATTATATGATGAAAAATTATTAGACGTTATAGTAGAGTAATTTTGCATTGTTTGTGCATAATAATTAAGTAAATCAAAGATTAGATTATGCACAATTTATTTATTTTTTTAAAGAATTATGCACAATTTCTAAACAAATTATTCAAAATGTATAATTTTTCTTAAAAAATATGTAGCATTTGTATTTTCTTTAAATGACACACATATGTATGTATGTATGTATGTATGTATGTACTCTAATCATTAAAAATGAATTATTATTTTTATTTTTATAGAAACTAACTTTAAGACAAAAAGTCATTTTAGGAAGTAATAAACAAAACATCTATTATAACTTTATAACCCAATAAATAATAATAAATAGTTACTTGAATTTTTCAATTTTCTAATTTATTCCTCACTATTTCTGTTAAGGTGATGATTGTATTTCAATTGTAAGTGGGTCCAAAAATGTTCGAGCAACAGATATTACTTGTGGACCAGGACATGGAATCAGGTTCATGACTCTCCTAATTGGAATTTTTTTTTATCTCCTTGAATTTTGAATTGATTGATTAAAAAATATGATATGGTAATTAATTTTTTTAATTGATTGGTTAAAAAATTTGATGTGGTAATTATTGTTTTGTAGCATTGGAAGCTTAGGAGGAGGTAACACAGAAGCAGAAGTGTCTAATGTGGAAGTGAACAGAGCCACACTAATAGGAACCACTAACGGAGTCAGAATAAAGACTTGGCAGGTAAATTTATGACTATTAGACACTCATTTATAAATTTTAAGGCTCATTTATAAATTATAAAGTAAAAAAAGTGGAATTGAAGCTACCGACTTAATTTAATTTTTTATTTTACCAAATTATTATCACAAGGCCTTCAAGTAAACATGGTTTATTTTATAATTTTGGGTAAATACTTGTTAATTTAGGGCCAAATTAGTTGCATTAGCACTATTATATTGGGAAGTTGACCATTGTTACATGGATTGATAATGGATTTTTTTTTTTATAATAATGGTTGATTTTCATTTCTAACTAAACATAAAGTTGGACATGCAATACTTTAACTAACTAAGTTTGGCAAGTTTACAAAGTCTACCAGTTTCTAACCTTATTACTTCAAACATGTCAATAACATTTTATATTGTGTTGTTCAACTTTCTTTAGTCCTTCTTCTTAACTAATTTAAACCAACAAAGAGTTTTTTTTCATTTTTTTTTGTTACAATTTTAGATGTCTATATACTTGATGAACATTAACTTTGACTAGACTATAAGAATGTATTTAAAAAATTTGATAAAAATTTATTCATTGTTATATTAGTAGTGTAGAGCTTAACTTGTTAGCTTTACTATTGATTTTGAAAGATAAACGTTTTTGGTCTAATGTCTTATTTTTCATACTATATATCTAAATGCAGGGAGGTTCTGGCTATGCAAGAAACATCAAATTTATGAACATAGTCATGCGAAATGTGACTAATCCCATAATCATAACTCAAAAATATTGTGACCAAAAAAAGAAATGTCAAGAGCAGGTAATTGTTTTTAAATGTCTTAACTACTTTTATTTTTGATATTATTTGAAGAAGAAAAAAATAAAATTAACGAACAAAATCAGAGGAAACAACCTAAACTTGAGTTTTAGGTCTAAAAACAAATTATCAAAAATGTGTATAGAGCAACACATGATGTGGGGTTTCAGAGCAACACAAGATATGGGCGTATAAAGAGCAATTATCTATAAAAAAATTCTTTTTCATTTTACAAATGTTTTCACCAAGCCCATGTTTTCATTCATTTTAATACCCCCCTTGCCTTGTTTTATTTGTTTAGTTTCATGGTTCAAAGTTCTTTTTATCTCTTTAAATATTTGTTACTAATTTAATTTTTTTTAAATGTAGTTATAATACTGTTTAAAATTTGTTACTAATGTAATCAAAATCATGGTCAAATTGCAGAACTCAGCTGTGGAATTGAGCAATGTGATGTACCAAAACATAAAAGGAACAAGTGCTTCAGAAGTTGCAATCAAACTTGAATGTAGCAAAGCTGTCCCATGCAAAGGAGTTCACTTGCAAGATGTGAAATTAACAGCACAAGGCAATGATAACACCACTGCCAAATGTGACAATGTTAATTATTCAAATAGTGGAATGTTCTTTCCTAAGTGTTACCCATGA
Protein sequence

>MS.gene77959.t1

MARAHHPSIAPSPQSQNTVNVDDYGAKSNDGQVDNEAFEMSWNEACSKGKVLVVPKHSVYHLKPITFSGPCQPNTALKIYGTIKAWPNISAYGADRKLWIKFENVTDLIVDGGGTINGNGKIWWENSCKVNESLPCKGAPTAVTFDECNNLKVDKIRFRNAQQMHIRIEGCNNVWASNLIVTAPGHSPNTDGIHITHSENVFITNSTIGTGDDCISIVSGSKNVRATDITCGPGHGISIGSLGGGNTEAEVSNVEVNRATLIGTTNGVRIKTWQGGSGYARNIKFMNIVMRNVTNPIIITQKYCDQKKKCQEQNSAVELSNVMYQNIKGTSASEVAIKLECSKAVPCKGVHLQDVKLTAQGNDNTTAKCDNVNYSNSGMFFPKCYP