Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78249.t1 | XP_003609685.2 | 96.7 | 121 | 4 | 0 | 1 | 121 | 1 | 121 | 6.10E-41 | 176.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78249.t1 | G7JLI7 | 96.7 | 121 | 4 | 0 | 1 | 121 | 1 | 121 | 4.4e-41 | 176.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050864 | MS.gene78249 | 0.815305 | 9.89E-52 | -1.69E-46 |
| MS.gene053083 | MS.gene78249 | 0.806167 | 9.38E-50 | -1.69E-46 |
| MS.gene05589 | MS.gene78249 | -0.809367 | 1.96E-50 | -1.69E-46 |
| MS.gene060076 | MS.gene78249 | 0.806687 | 7.29E-50 | -1.69E-46 |
| MS.gene06042 | MS.gene78249 | 0.810482 | 1.13E-50 | -1.69E-46 |
| MS.gene061044 | MS.gene78249 | 0.812427 | 4.26E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78249.t1 | MTR_4g120020 | 96.694 | 121 | 4 | 0 | 1 | 121 | 1 | 121 | 8.48e-78 | 225 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene78249.t1 | AT3G06890 | 75.591 | 127 | 25 | 1 | 1 | 121 | 1 | 127 | 1.50e-31 | 108 |
Find 33 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACCACCTTGTTGTCTTTC+TGG | 0.014264 | 8.4:-11962065 | None:intergenic |
| GTAATAGTTCCTTTCCTTCT+CGG | 0.355214 | 8.4:+11962134 | MS.gene78249:CDS |
| AGGTTCACCGGTGGAGAAAA+TGG | 0.355541 | 8.4:-11962285 | None:intergenic |
| TGGAACCGTGTTTCGATCTT+CGG | 0.370599 | 8.4:+11962380 | MS.gene78249:CDS |
| GATACTATTCATCGTGTTAG+TGG | 0.389930 | 8.4:+11962308 | MS.gene78249:CDS |
| CCTTTCCTTCTCGGAGATCA+AGG | 0.391728 | 8.4:+11962143 | MS.gene78249:CDS |
| AGCTATCACAGAAGCTATAT+CGG | 0.433454 | 8.4:+11962169 | MS.gene78249:CDS |
| CTAACACGATGAATAGTATC+AGG | 0.440753 | 8.4:-11962305 | None:intergenic |
| AGCAGTGGTGGTAGCTATAG+TGG | 0.468105 | 8.4:+11962112 | MS.gene78249:CDS |
| ATCGTGAGAAGAAGGATGAT+TGG | 0.488963 | 8.4:-11962224 | None:intergenic |
| GGAACTGAATCGTGAGAAGA+AGG | 0.507982 | 8.4:-11962232 | None:intergenic |
| GAAAGACAACAAGGTGGTGG+AGG | 0.540517 | 8.4:+11962068 | MS.gene78249:CDS |
| CGTGTTAGTGGATCACCGTT+CGG | 0.558827 | 8.4:+11962320 | MS.gene78249:CDS |
| CCTTGATCTCCGAGAAGGAA+AGG | 0.562958 | 8.4:-11962143 | None:intergenic |
| CCAGAAAGACAACAAGGTGG+TGG | 0.583317 | 8.4:+11962065 | MS.gene78249:CDS |
| ATGAATAGTATCAGGTTCAC+CGG | 0.583799 | 8.4:-11962297 | None:intergenic |
| TCGGAGCTGCTAAGTCCACT+TGG | 0.597655 | 8.4:+11962188 | MS.gene78249:CDS |
| AACCGTGTTTCGATCTTCGG+TGG | 0.599415 | 8.4:+11962383 | MS.gene78249:CDS |
| CGGTATTCTTCTAGCAGTGG+TGG | 0.606734 | 8.4:+11962100 | MS.gene78249:CDS |
| CTTCGCCTTGATCTCCGAGA+AGG | 0.615486 | 8.4:-11962148 | None:intergenic |
| AGGAAAAGAGCAACACCGAA+CGG | 0.621281 | 8.4:-11962335 | None:intergenic |
| CAGTTCCTCTCATCTGAACG+TGG | 0.622156 | 8.4:+11962248 | MS.gene78249:CDS |
| AAAGCACAAGAATGAGAACA+AGG | 0.624793 | 8.4:-11962355 | None:intergenic |
| ACGATCCACGTTCAGATGAG+AGG | 0.629269 | 8.4:-11962253 | None:intergenic |
| ACACGGTATTCTTCTAGCAG+TGG | 0.660684 | 8.4:+11962097 | MS.gene78249:CDS |
| GGTAGAAGAAGAAGACCAAG+TGG | 0.662368 | 8.4:-11962203 | None:intergenic |
| AATAGTATCAGGTTCACCGG+TGG | 0.662862 | 8.4:-11962294 | None:intergenic |
| ATGTATCCAGAAAGACAACA+AGG | 0.678373 | 8.4:+11962059 | MS.gene78249:CDS |
| CACCACCGAAGATCGAAACA+CGG | 0.699903 | 8.4:-11962385 | None:intergenic |
| GGTGGTGGAGGAGCACCACA+CGG | 0.704308 | 8.4:+11962080 | MS.gene78249:CDS |
| TTCACCGGTGGAGAAAATGG+CGG | 0.726368 | 8.4:-11962282 | None:intergenic |
| TATCCAGAAAGACAACAAGG+TGG | 0.796650 | 8.4:+11962062 | MS.gene78249:CDS |
| ACTGCTAGAAGAATACCGTG+TGG | 0.809004 | 8.4:-11962095 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAGCACAAGAATGAGAACA+AGG | - | chr8.4:11962358-11962377 | None:intergenic | 35.0% | |
| AGCTATCACAGAAGCTATAT+CGG | + | chr8.4:11962169-11962188 | MS.gene78249:CDS | 35.0% | |
| ATGAATAGTATCAGGTTCAC+CGG | - | chr8.4:11962300-11962319 | None:intergenic | 35.0% | |
| ATGTATCCAGAAAGACAACA+AGG | + | chr8.4:11962059-11962078 | MS.gene78249:CDS | 35.0% | |
| CTAACACGATGAATAGTATC+AGG | - | chr8.4:11962308-11962327 | None:intergenic | 35.0% | |
| GATACTATTCATCGTGTTAG+TGG | + | chr8.4:11962308-11962327 | MS.gene78249:CDS | 35.0% | |
| GTAATAGTTCCTTTCCTTCT+CGG | + | chr8.4:11962134-11962153 | MS.gene78249:CDS | 35.0% | |
| ! | TTCTCATTCTTGTGCTTTTG+TGG | + | chr8.4:11962360-11962379 | MS.gene78249:CDS | 35.0% |
| ATCGTGAGAAGAAGGATGAT+TGG | - | chr8.4:11962227-11962246 | None:intergenic | 40.0% | |
| TATCCAGAAAGACAACAAGG+TGG | + | chr8.4:11962062-11962081 | MS.gene78249:CDS | 40.0% | |
| AATAGTATCAGGTTCACCGG+TGG | - | chr8.4:11962297-11962316 | None:intergenic | 45.0% | |
| ACACGGTATTCTTCTAGCAG+TGG | + | chr8.4:11962097-11962116 | MS.gene78249:CDS | 45.0% | |
| ACTGCTAGAAGAATACCGTG+TGG | - | chr8.4:11962098-11962117 | None:intergenic | 45.0% | |
| AGGAAAAGAGCAACACCGAA+CGG | - | chr8.4:11962338-11962357 | None:intergenic | 45.0% | |
| GGAACTGAATCGTGAGAAGA+AGG | - | chr8.4:11962235-11962254 | None:intergenic | 45.0% | |
| GGTAGAAGAAGAAGACCAAG+TGG | - | chr8.4:11962206-11962225 | None:intergenic | 45.0% | |
| TGGAACCGTGTTTCGATCTT+CGG | + | chr8.4:11962380-11962399 | MS.gene78249:CDS | 45.0% | |
| AACCGTGTTTCGATCTTCGG+TGG | + | chr8.4:11962383-11962402 | MS.gene78249:CDS | 50.0% | |
| ACGATCCACGTTCAGATGAG+AGG | - | chr8.4:11962256-11962275 | None:intergenic | 50.0% | |
| AGCAGTGGTGGTAGCTATAG+TGG | + | chr8.4:11962112-11962131 | MS.gene78249:CDS | 50.0% | |
| AGGTTCACCGGTGGAGAAAA+TGG | - | chr8.4:11962288-11962307 | None:intergenic | 50.0% | |
| CACCACCGAAGATCGAAACA+CGG | - | chr8.4:11962388-11962407 | None:intergenic | 50.0% | |
| CAGTTCCTCTCATCTGAACG+TGG | + | chr8.4:11962248-11962267 | MS.gene78249:CDS | 50.0% | |
| CCAGAAAGACAACAAGGTGG+TGG | + | chr8.4:11962065-11962084 | MS.gene78249:CDS | 50.0% | |
| CCTTGATCTCCGAGAAGGAA+AGG | - | chr8.4:11962146-11962165 | None:intergenic | 50.0% | |
| CCTTTCCTTCTCGGAGATCA+AGG | + | chr8.4:11962143-11962162 | MS.gene78249:CDS | 50.0% | |
| CGGTATTCTTCTAGCAGTGG+TGG | + | chr8.4:11962100-11962119 | MS.gene78249:CDS | 50.0% | |
| TTCACCGGTGGAGAAAATGG+CGG | - | chr8.4:11962285-11962304 | None:intergenic | 50.0% | |
| ! | CCACCACCTTGTTGTCTTTC+TGG | - | chr8.4:11962068-11962087 | None:intergenic | 50.0% |
| ! | CGTGTTAGTGGATCACCGTT+CGG | + | chr8.4:11962320-11962339 | MS.gene78249:CDS | 50.0% |
| ! | GAAAGACAACAAGGTGGTGG+AGG | + | chr8.4:11962068-11962087 | MS.gene78249:CDS | 50.0% |
| !! | ATCTCCGCCATTTTCTCCAC+CGG | + | chr8.4:11962278-11962297 | MS.gene78249:CDS | 50.0% |
| CTTCGCCTTGATCTCCGAGA+AGG | - | chr8.4:11962151-11962170 | None:intergenic | 55.0% | |
| TCGGAGCTGCTAAGTCCACT+TGG | + | chr8.4:11962188-11962207 | MS.gene78249:CDS | 55.0% | |
| !! | GGTGGTGGAGGAGCACCACA+CGG | + | chr8.4:11962080-11962099 | MS.gene78249:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 11962059 | 11962424 | 11962059 | ID=MS.gene78249 |
| chr8.4 | mRNA | 11962059 | 11962424 | 11962059 | ID=MS.gene78249.t1;Parent=MS.gene78249 |
| chr8.4 | exon | 11962059 | 11962424 | 11962059 | ID=MS.gene78249.t1.exon1;Parent=MS.gene78249.t1 |
| chr8.4 | CDS | 11962059 | 11962424 | 11962059 | ID=cds.MS.gene78249.t1;Parent=MS.gene78249.t1 |
| Gene Sequence |
| Protein sequence |
