Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79444.t1 | XP_003626244.1 | 92.9 | 156 | 11 | 0 | 1 | 156 | 1 | 156 | 8.60E-83 | 316.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79444.t1 | I6YGS0 | 41.4 | 152 | 86 | 3 | 7 | 156 | 7 | 157 | 8.5e-31 | 134.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79444.t1 | A2Q321 | 92.9 | 156 | 11 | 0 | 1 | 156 | 1 | 156 | 6.2e-83 | 316.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049265 | MS.gene79444 | 0.803084 | 4.13E-49 | -1.69E-46 |
| MS.gene05240 | MS.gene79444 | 0.800404 | 1.46E-48 | -1.69E-46 |
| MS.gene054181 | MS.gene79444 | 0.807992 | 3.85E-50 | -1.69E-46 |
| MS.gene054290 | MS.gene79444 | 0.805142 | 1.54E-49 | -1.69E-46 |
| MS.gene057544 | MS.gene79444 | 0.837393 | 5.34E-57 | -1.69E-46 |
| MS.gene058218 | MS.gene79444 | 0.831485 | 1.63E-55 | -1.69E-46 |
| MS.gene059037 | MS.gene79444 | 0.801487 | 8.80E-49 | -1.69E-46 |
| MS.gene059402 | MS.gene79444 | 0.809669 | 1.69E-50 | -1.69E-46 |
| MS.gene059953 | MS.gene79444 | 0.80861 | 2.85E-50 | -1.69E-46 |
| MS.gene059955 | MS.gene79444 | 0.80935 | 1.98E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79444.t1 | MTR_7g113030 | 92.949 | 156 | 11 | 0 | 1 | 156 | 1 | 156 | 1.22e-108 | 312 |
| MS.gene79444.t1 | MTR_7g113050 | 91.667 | 156 | 13 | 0 | 1 | 156 | 1 | 156 | 1.21e-106 | 304 |
| MS.gene79444.t1 | MTR_7g113050 | 91.667 | 156 | 13 | 0 | 1 | 156 | 1 | 156 | 7.45e-106 | 305 |
| MS.gene79444.t1 | MTR_7g112615 | 87.342 | 158 | 20 | 0 | 1 | 158 | 1 | 158 | 6.90e-103 | 292 |
| MS.gene79444.t1 | MTR_7g112615 | 87.821 | 156 | 19 | 0 | 1 | 156 | 1 | 156 | 1.01e-102 | 294 |
| MS.gene79444.t1 | MTR_7g112615 | 87.821 | 156 | 19 | 0 | 1 | 156 | 1 | 156 | 7.99e-102 | 295 |
| MS.gene79444.t1 | MTR_7g112960 | 85.256 | 156 | 23 | 0 | 1 | 156 | 1 | 156 | 1.18e-98 | 287 |
| MS.gene79444.t1 | MTR_7g112970 | 82.911 | 158 | 27 | 0 | 1 | 158 | 1 | 158 | 3.38e-98 | 286 |
| MS.gene79444.t1 | MTR_7g113020 | 83.974 | 156 | 25 | 0 | 1 | 156 | 1 | 156 | 1.63e-97 | 284 |
| MS.gene79444.t1 | MTR_7g112963 | 84.615 | 156 | 24 | 0 | 1 | 156 | 1 | 156 | 2.00e-97 | 284 |
| MS.gene79444.t1 | MTR_7g112620 | 84.615 | 156 | 24 | 0 | 1 | 156 | 1 | 156 | 5.24e-97 | 283 |
| MS.gene79444.t1 | MTR_7g113010 | 83.333 | 156 | 26 | 0 | 1 | 156 | 1 | 156 | 6.14e-95 | 286 |
| MS.gene79444.t1 | MTR_1g083480 | 73.585 | 159 | 38 | 2 | 1 | 156 | 1 | 158 | 5.89e-87 | 252 |
| MS.gene79444.t1 | MTR_4g005160 | 72.956 | 159 | 39 | 2 | 1 | 156 | 1 | 158 | 2.19e-85 | 253 |
| MS.gene79444.t1 | MTR_7g108825 | 66.026 | 156 | 52 | 1 | 1 | 156 | 1 | 155 | 1.12e-69 | 211 |
| MS.gene79444.t1 | MTR_4g120240 | 63.924 | 158 | 54 | 2 | 1 | 156 | 37 | 193 | 1.51e-69 | 214 |
| MS.gene79444.t1 | MTR_7g108825 | 66.026 | 156 | 52 | 1 | 1 | 156 | 1 | 155 | 1.23e-68 | 211 |
| MS.gene79444.t1 | MTR_3g024250 | 90.654 | 107 | 10 | 0 | 46 | 152 | 1 | 107 | 3.64e-68 | 203 |
| MS.gene79444.t1 | MTR_8g076680 | 53.205 | 156 | 72 | 1 | 1 | 156 | 3 | 157 | 5.32e-61 | 188 |
| MS.gene79444.t1 | MTR_8g076680 | 53.205 | 156 | 72 | 1 | 1 | 156 | 3 | 157 | 3.62e-60 | 189 |
| MS.gene79444.t1 | MTR_4g089060 | 39.103 | 156 | 90 | 1 | 1 | 156 | 1 | 151 | 2.75e-38 | 130 |
| MS.gene79444.t1 | MTR_4g089060 | 40.373 | 161 | 90 | 2 | 1 | 160 | 1 | 156 | 3.37e-38 | 133 |
| MS.gene79444.t1 | MTR_4g089060 | 39.103 | 156 | 90 | 1 | 1 | 156 | 1 | 151 | 9.34e-38 | 131 |
| MS.gene79444.t1 | MTR_4g089040 | 39.103 | 156 | 90 | 1 | 1 | 156 | 1 | 151 | 9.37e-36 | 126 |
| MS.gene79444.t1 | MTR_2g090240 | 37.821 | 156 | 93 | 1 | 1 | 156 | 1 | 152 | 7.53e-35 | 124 |
| MS.gene79444.t1 | MTR_2g090245 | 37.179 | 156 | 94 | 2 | 1 | 156 | 1 | 152 | 2.85e-33 | 119 |
| MS.gene79444.t1 | MTR_7g112910 | 51.765 | 85 | 25 | 1 | 72 | 156 | 1 | 69 | 7.82e-24 | 92.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene79444.t1 | AT3G05600 | 69.811 | 159 | 45 | 2 | 1 | 156 | 1 | 159 | 1.28e-79 | 236 |
| MS.gene79444.t1 | AT3G05600 | 69.811 | 159 | 45 | 2 | 1 | 156 | 1 | 159 | 9.28e-79 | 237 |
| MS.gene79444.t1 | AT4G02340 | 63.462 | 156 | 56 | 1 | 1 | 156 | 1 | 155 | 7.34e-72 | 219 |
| MS.gene79444.t1 | AT2G26740 | 62.893 | 159 | 53 | 3 | 4 | 156 | 1 | 159 | 6.92e-67 | 206 |
| MS.gene79444.t1 | AT4G15955 | 59.259 | 162 | 56 | 2 | 5 | 156 | 6 | 167 | 2.18e-66 | 200 |
| MS.gene79444.t1 | AT4G15955 | 59.259 | 162 | 56 | 2 | 5 | 156 | 6 | 167 | 2.79e-66 | 200 |
| MS.gene79444.t1 | AT4G15955 | 59.146 | 164 | 57 | 2 | 5 | 158 | 6 | 169 | 3.00e-65 | 201 |
| MS.gene79444.t1 | AT2G26750 | 62.025 | 158 | 55 | 3 | 4 | 156 | 1 | 158 | 1.48e-64 | 200 |
| MS.gene79444.t1 | AT4G15960 | 56.173 | 162 | 65 | 2 | 1 | 156 | 51 | 212 | 1.23e-61 | 194 |
| MS.gene79444.t1 | AT3G51000 | 55.195 | 154 | 67 | 2 | 4 | 156 | 5 | 157 | 1.79e-57 | 182 |
Find 0 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATTATATATGTAGATTTC+AGG | + | 21092:2437-2456 | MS.gene79444:CDS | 15.0% |
| ! | AAAATGCATATTGCTGAAAA+AGG | + | 21092:2033-2052 | MS.gene79444:CDS | 25.0% |
| ATTGCTGAAAAAGGAAAACA+AGG | + | 21092:2042-2061 | MS.gene79444:CDS | 30.0% | |
| GAAAATCAATAAGTGCAACA+AGG | - | 21092:2242-2261 | MS.gene79444:intergenic | 30.0% | |
| ! | CATCAACTGTCTTGATTTTA+GGG | - | 21092:2392-2411 | MS.gene79444:intergenic | 30.0% |
| !! | AACATAGGCTTTGATTTTGT+CGG | - | 21092:2340-2359 | MS.gene79444:intergenic | 30.0% |
| !!! | GTTGCACTTATTGATTTTCT+GGG | + | 21092:2243-2262 | MS.gene79444:CDS | 30.0% |
| !!! | TCTTGATTTTAGGGTTTCTT+CGG | - | 21092:2383-2402 | MS.gene79444:intergenic | 30.0% |
| !!! | TGTTGCACTTATTGATTTTC+TGG | + | 21092:2242-2261 | MS.gene79444:CDS | 30.0% |
| CCATGAAGAAACAAGACAAT+TGG | - | 21092:2069-2088 | MS.gene79444:intergenic | 35.0% | |
| GCTTATTGAAGATGGAGAAT+CGG | - | 21092:2190-2209 | MS.gene79444:intergenic | 35.0% | |
| TGTTGATCAAGTGTTTCTTG+TGG | + | 21092:2266-2285 | MS.gene79444:CDS | 35.0% | |
| ! | GCATCAACTGTCTTGATTTT+AGG | - | 21092:2393-2412 | MS.gene79444:intergenic | 35.0% |
| !! | ACATAGGCTTTGATTTTGTC+GGG | - | 21092:2339-2358 | MS.gene79444:intergenic | 35.0% |
| !! | CCAATTGTCTTGTTTCTTCA+TGG | + | 21092:2066-2085 | MS.gene79444:CDS | 35.0% |
| AGGAATAGAACACAGAACAG+TGG | + | 21092:1996-2015 | MS.gene79444:CDS | 40.0% | |
| CTTCAATAAGCAGCTACACA+GGG | + | 21092:2199-2218 | MS.gene79444:CDS | 40.0% | |
| GTGTAGCTGCTTATTGAAGA+TGG | - | 21092:2198-2217 | MS.gene79444:intergenic | 40.0% | |
| GTTTCTTGTGGCTCATGATT+GGG | + | 21092:2278-2297 | MS.gene79444:CDS | 40.0% | |
| TACACAGGGTTTCATATGGT+TGG | + | 21092:2213-2232 | MS.gene79444:CDS | 40.0% | |
| TCTTCAATAAGCAGCTACAC+AGG | + | 21092:2198-2217 | MS.gene79444:CDS | 40.0% | |
| TGTTTCTTGTGGCTCATGAT+TGG | + | 21092:2277-2296 | MS.gene79444:CDS | 40.0% | |
| TTTCTTGTGGCTCATGATTG+GGG | + | 21092:2279-2298 | MS.gene79444:CDS | 40.0% | |
| !! | CATAGGCTTTGATTTTGTCG+GGG | - | 21092:2338-2357 | MS.gene79444:intergenic | 40.0% |
| AGCTAAGAGCTACAATCTGG+TGG | - | 21092:2119-2138 | MS.gene79443:intergenic | 45.0% | |
| ATCTCCATAAGCAGCATGCA+TGG | - | 21092:2415-2434 | MS.gene79444:intergenic | 45.0% | |
| ATTGTAGCTCTTAGCTCCCT+CGG | + | 21092:2123-2142 | MS.gene79444:CDS | 45.0% | |
| CACAGAACAGTGGAAGTGAA+TGG | + | 21092:2006-2025 | MS.gene79444:CDS | 45.0% | |
| CAGCTACACAGGGTTTCATA+TGG | + | 21092:2209-2228 | MS.gene79444:CDS | 45.0% | |
| GGGAACACTGAGACAAACAT+AGG | - | 21092:2355-2374 | MS.gene79444:intergenic | 45.0% | |
| TCCTGGAACTCTGGTATTCA+TGG | + | 21092:2091-2110 | MS.gene79444:CDS | 45.0% | |
| TTAGGGTTTCTTCGGAGCAA+GGG | - | 21092:2375-2394 | MS.gene79444:intergenic | 45.0% | |
| ! | ATTGGGGTGCTATCATTGGT+TGG | + | 21092:2295-2314 | MS.gene79444:CDS | 45.0% |
| ! | CATGATTGGGGTGCTATCAT+TGG | + | 21092:2291-2310 | MS.gene79444:CDS | 45.0% |
| ! | CTTGTTTCTTCATGGCTTCC+TGG | + | 21092:2074-2093 | MS.gene79444:CDS | 45.0% |
| ! | TTTAGGGTTTCTTCGGAGCA+AGG | - | 21092:2376-2395 | MS.gene79444:intergenic | 45.0% |
| GCCATGAATACCAGAGTTCC+AGG | - | 21092:2095-2114 | MS.gene79444:intergenic | 50.0% | |
| GGGAGCTAAGAGCTACAATC+TGG | - | 21092:2122-2141 | MS.gene79444:intergenic | 50.0% | |
| TCACCATAGCCACGAAGATC+AGG | - | 21092:2165-2184 | MS.gene79444:intergenic | 50.0% | |
| TTCATGGCTTCCTGGAACTC+TGG | + | 21092:2082-2101 | MS.gene79444:CDS | 50.0% | |
| ! | GATGCCATGCATGCTGCTTA+TGG | + | 21092:2408-2427 | MS.gene79444:CDS | 50.0% |
| GCTCCTGATCTTCGTGGCTA+TGG | + | 21092:2159-2178 | MS.gene79444:CDS | 55.0% | |
| TGTCGGGGCGAAACATGCAA+AGG | - | 21092:2323-2342 | MS.gene79444:intergenic | 55.0% | |
| GAAGATCAGGAGCCACGGCA+TGG | - | 21092:2152-2171 | MS.gene79444:intergenic | 60.0% | |
| GCCACGAAGATCAGGAGCCA+CGG | - | 21092:2157-2176 | MS.gene79444:intergenic | 60.0% | |
| AGCCACGGCATGGTAGCCGA+GGG | - | 21092:2142-2161 | MS.gene79443:intergenic | 65.0% | |
| GCCGTGGCTCCTGATCTTCG+TGG | + | 21092:2153-2172 | MS.gene79444:CDS | 65.0% | |
| CTCCCTCGGCTACCATGCCG+TGG | + | 21092:2137-2156 | MS.gene79444:CDS | 70.0% | |
| GAGCCACGGCATGGTAGCCG+AGG | - | 21092:2143-2162 | MS.gene79444:intergenic | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 21092 | gene | 1991 | 2476 | 1991 | ID=MS.gene79444 |
| 21092 | mRNA | 1991 | 2476 | 1991 | ID=MS.gene79444.t1;Parent=MS.gene79444 |
| 21092 | exon | 1991 | 2476 | 1991 | ID=MS.gene79444.t1.exon1;Parent=MS.gene79444.t1 |
| 21092 | CDS | 1991 | 2476 | 1991 | ID=cds.MS.gene79444.t1;Parent=MS.gene79444.t1 |
| Gene Sequence |
| Protein sequence |
