AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene059037


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene059037.t1 MTR_2g035100 100.000 157 0 0 1 157 1 157 3.24e-110 310
MS.gene059037.t1 MTR_2g035105 98.726 157 2 0 1 157 1 157 9.40e-109 306
MS.gene059037.t1 MTR_2g035130 87.898 157 19 0 1 157 1 157 1.75e-96 275
MS.gene059037.t1 MTR_2g035150 85.350 157 23 0 1 157 1 157 3.70e-94 270
MS.gene059037.t1 MTR_2g035120 84.076 157 25 0 1 157 1 157 6.07e-94 269
MS.gene059037.t1 MTR_6g033450 71.975 157 44 0 1 157 1 157 8.33e-80 233
MS.gene059037.t1 MTR_4g120970 71.975 157 44 0 1 157 1 157 8.33e-80 233
MS.gene059037.t1 MTR_2g035320 65.605 157 54 0 1 157 1 157 2.34e-69 207
MS.gene059037.t1 MTR_2g035190 61.783 157 60 0 1 157 1 157 5.22e-66 198
MS.gene059037.t1 MTR_2g035210 61.146 157 61 0 1 157 1 157 8.15e-65 195
MS.gene059037.t1 MTR_2g035220 61.783 157 60 0 1 157 1 157 7.77e-64 192
MS.gene059037.t1 MTR_2g035170 60.510 157 62 0 1 157 1 157 4.99e-63 191
MS.gene059037.t1 MTR_2g435310 41.026 156 91 1 1 155 1 156 1.14e-39 131
MS.gene059037.t1 MTR_4g120760 40.385 156 92 1 1 155 1 156 2.35e-37 125
MS.gene059037.t1 MTR_4g120760 40.385 156 92 1 1 155 1 156 2.37e-37 125
MS.gene059037.t1 MTR_2g035320 63.542 96 35 0 62 157 27 122 5.57e-37 123
MS.gene059037.t1 MTR_4g120950 44.720 161 82 5 1 157 1 158 5.30e-33 115
MS.gene059037.t1 MTR_4g120940 62.500 88 30 1 70 157 35 119 2.73e-30 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 41 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCAATAACCTTTGGAATA+AGG 0.200219 2.4:+56077548 None:intergenic
AACGAAAGTAAGTTTCTTGA+TGG 0.287513 2.4:+56077480 None:intergenic
AGCTATATGCTTTAGTAATC+AGG 0.289844 2.4:+56076897 None:intergenic
ACCAAGTGAAGAGGAAATTA+AGG 0.303784 2.4:-56076979 MS.gene059037:CDS
CTTCAACAATTTCAATACTT+TGG 0.342783 2.4:+56077521 None:intergenic
CCCTTAATTTCCTCTTCACT+TGG 0.359117 2.4:+56076978 None:intergenic
GAGATCTTCTCCACTGTGTC+TGG 0.364866 2.4:+56077086 None:intergenic
AAGAAACTTACTTTCGTTGA+AGG 0.370871 2.4:-56077475 MS.gene059037:intron
AGTAAGTTTCTTGATGGTTC+CGG 0.371837 2.4:+56077486 None:intergenic
GGCTCTTGAAGGTTACGTTT+TGG 0.372797 2.4:-56076925 MS.gene059037:CDS
CAAAGTATTGAAATTGTTGA+AGG 0.421501 2.4:-56077520 MS.gene059037:CDS
TGGATGGCATCAATAACCTT+TGG 0.428619 2.4:+56077541 None:intergenic
AACAATTTCAATACTTTGGA+TGG 0.439896 2.4:+56077525 None:intergenic
ATTGAAATTGTTGAAGGAAA+CGG 0.445615 2.4:-56077514 MS.gene059037:CDS
TTTGAGTCTAAATTGTCTGC+AGG 0.453113 2.4:-56077062 MS.gene059037:CDS
TTTGCAATGGATCCTCCATC+TGG 0.453770 2.4:+56077038 None:intergenic
AAATTAAGGGTGGCAAAGCT+AGG 0.456128 2.4:-56076965 MS.gene059037:CDS
GCTTACAACTACAGCATAGT+TGG 0.463271 2.4:-56077122 MS.gene059037:CDS
CATGGGTGTATTCAACTTTG+AGG 0.475033 2.4:-56077635 MS.gene059037:CDS
TACAGCATAGTTGGTGGTGG+TGG 0.475041 2.4:-56077113 MS.gene059037:CDS
AGAGCTTTGTAAAGTCTAGC+AGG 0.480944 2.4:+56077589 None:intergenic
GGTGGCAAAGCTAGGGGTGA+TGG 0.489081 2.4:-56076957 MS.gene059037:CDS
ACTGTGAAATACTTCACAAA+AGG 0.498744 2.4:-56077011 MS.gene059037:CDS
AATTAAGGGTGGCAAAGCTA+GGG 0.501100 2.4:-56076964 MS.gene059037:CDS
CCAAGTGAAGAGGAAATTAA+GGG 0.521068 2.4:-56076978 MS.gene059037:CDS
TTCTGATAACCTTATTCCAA+AGG 0.526563 2.4:-56077557 MS.gene059037:CDS
AAAGTATGATTTGCACAAAG+TGG 0.527363 2.4:-56077168 MS.gene059037:CDS
TTTCACAGTAAGCTTTGCAA+TGG 0.527944 2.4:+56077025 None:intergenic
GTTGAAGGAAACGGTGGTGC+CGG 0.528042 2.4:-56077505 MS.gene059037:CDS
TGCAGACAATTTAGACTCAA+AGG 0.553148 2.4:+56077064 None:intergenic
AAATTGTCTGCAGGGCCAGA+TGG 0.558648 2.4:-56077053 MS.gene059037:CDS
TTGAGTCTAAATTGTCTGCA+GGG 0.579973 2.4:-56077061 MS.gene059037:CDS
TGATGCTGCACCAAGTGAAG+AGG 0.603594 2.4:-56076988 MS.gene059037:CDS
TTGTCTGCAGGGCCAGATGG+AGG 0.618761 2.4:-56077050 MS.gene059037:CDS
TACAACTACAGCATAGTTGG+TGG 0.619903 2.4:-56077119 MS.gene059037:CDS
AACTACAGCATAGTTGGTGG+TGG 0.623337 2.4:-56077116 MS.gene059037:CDS
AGCAGGAGCTACAATAGATG+TGG 0.641681 2.4:+56077606 None:intergenic
ATTAAGGGTGGCAAAGCTAG+GGG 0.650783 2.4:-56076963 MS.gene059037:CDS
AGTGAAGAGGAAATTAAGGG+TGG 0.698075 2.4:-56076975 MS.gene059037:CDS
GAAATTGTTGAAGGAAACGG+TGG 0.726108 2.4:-56077511 MS.gene059037:CDS
TGGTGGACTTCCAGACACAG+TGG 0.739823 2.4:-56077096 MS.gene059037:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATAATAAAAGAAAAG+AGG + chr2.4:56077180-56077199 None:intergenic 10.0%
!!! TCTTTTCTTTTATTATATAT+TGG - chr2.4:56077179-56077198 MS.gene059037:CDS 10.0%
!! ATATATGTGAAAAATTGAAG+TGG - chr2.4:56077097-56077116 MS.gene059037:CDS 20.0%
! AACAAGAATCAAACATTTGA+TGG + chr2.4:56077140-56077159 None:intergenic 25.0%
! AACAATTTCAATACTTTGGA+TGG + chr2.4:56077020-56077039 None:intergenic 25.0%
! ATTGAAATTGTTGAAGGAAA+CGG - chr2.4:56077028-56077047 MS.gene059037:CDS 25.0%
! CTTCAACAATTTCAATACTT+TGG + chr2.4:56077024-56077043 None:intergenic 25.0%
! TTTCACAATTACTAACATGA+TGG + chr2.4:56077326-56077345 None:intergenic 25.0%
!!! CAAAGTATTGAAATTGTTGA+AGG - chr2.4:56077022-56077041 MS.gene059037:CDS 25.0%
AACGAAAGTAAGTTTCTTGA+TGG + chr2.4:56077065-56077084 None:intergenic 30.0%
AAGAAACTTACTTTCGTTGA+AGG - chr2.4:56077067-56077086 MS.gene059037:CDS 30.0%
ACTGTGAAATACTTCACAAA+AGG - chr2.4:56077531-56077550 MS.gene059037:CDS 30.0%
TTCTGATAACCTTATTCCAA+AGG - chr2.4:56076985-56077004 MS.gene059037:CDS 30.0%
! AAAGTATGATTTGCACAAAG+TGG - chr2.4:56077374-56077393 MS.gene059037:intron 30.0%
!! CATCAATAACCTTTGGAATA+AGG + chr2.4:56076997-56077016 None:intergenic 30.0%
ACCAAGTGAAGAGGAAATTA+AGG - chr2.4:56077563-56077582 MS.gene059037:CDS 35.0%
ATGTGAAAAATTGAAGTGGC+AGG - chr2.4:56077101-56077120 MS.gene059037:CDS 35.0%
CCAAGTGAAGAGGAAATTAA+GGG - chr2.4:56077564-56077583 MS.gene059037:CDS 35.0%
GAAACGAATCAATTATGTGC+AGG - chr2.4:56077342-56077361 MS.gene059037:intron 35.0%
TGCAGACAATTTAGACTCAA+AGG + chr2.4:56077481-56077500 None:intergenic 35.0%
TTGAGTCTAAATTGTCTGCA+GGG - chr2.4:56077481-56077500 MS.gene059037:CDS 35.0%
TTTCACAGTAAGCTTTGCAA+TGG + chr2.4:56077520-56077539 None:intergenic 35.0%
TTTGAGTCTAAATTGTCTGC+AGG - chr2.4:56077480-56077499 MS.gene059037:CDS 35.0%
! AGTAAGTTTCTTGATGGTTC+CGG + chr2.4:56077059-56077078 None:intergenic 35.0%
!! TAACATGATGGACATTCACT+TGG + chr2.4:56077314-56077333 None:intergenic 35.0%
AAATTAAGGGTGGCAAAGCT+AGG - chr2.4:56077577-56077596 MS.gene059037:CDS 40.0%
AATTAAGGGTGGCAAAGCTA+GGG - chr2.4:56077578-56077597 MS.gene059037:CDS 40.0%
ACGAATCAATTATGTGCAGG+TGG - chr2.4:56077345-56077364 MS.gene059037:intron 40.0%
AGTGAAGAGGAAATTAAGGG+TGG - chr2.4:56077567-56077586 MS.gene059037:CDS 40.0%
CCCTTAATTTCCTCTTCACT+TGG + chr2.4:56077567-56077586 None:intergenic 40.0%
GAAATTGTTGAAGGAAACGG+TGG - chr2.4:56077031-56077050 MS.gene059037:CDS 40.0%
GACATTCACTTGGTTCAACT+AGG + chr2.4:56077304-56077323 None:intergenic 40.0%
GCTTACAACTACAGCATAGT+TGG - chr2.4:56077420-56077439 MS.gene059037:intron 40.0%
TACAACTACAGCATAGTTGG+TGG - chr2.4:56077423-56077442 MS.gene059037:intron 40.0%
TGGATGGCATCAATAACCTT+TGG + chr2.4:56077004-56077023 None:intergenic 40.0%
! AGAGCTTTGTAAAGTCTAGC+AGG + chr2.4:56076956-56076975 None:intergenic 40.0%
AGCAGGAGCTACAATAGATG+TGG + chr2.4:56076939-56076958 None:intergenic 45.0%
ATTAAGGGTGGCAAAGCTAG+GGG - chr2.4:56077579-56077598 MS.gene059037:CDS 45.0%
TTTGCAATGGATCCTCCATC+TGG + chr2.4:56077507-56077526 None:intergenic 45.0%
! GGCTCTTGAAGGTTACGTTT+TGG - chr2.4:56077617-56077636 MS.gene059037:CDS 45.0%
!! AACTACAGCATAGTTGGTGG+TGG - chr2.4:56077426-56077445 MS.gene059037:intron 45.0%
!! GGTCTTTTCAAGGCTCTTGA+AGG - chr2.4:56077606-56077625 MS.gene059037:CDS 45.0%
!! TAGGGGTGATGGTCTTTTCA+AGG - chr2.4:56077596-56077615 MS.gene059037:CDS 45.0%
AAATTGTCTGCAGGGCCAGA+TGG - chr2.4:56077489-56077508 MS.gene059037:CDS 50.0%
GAGATCTTCTCCACTGTGTC+TGG + chr2.4:56077459-56077478 None:intergenic 50.0%
TGATGCTGCACCAAGTGAAG+AGG - chr2.4:56077554-56077573 MS.gene059037:CDS 50.0%
!! TACAGCATAGTTGGTGGTGG+TGG - chr2.4:56077429-56077448 MS.gene059037:intron 50.0%
GTTGAAGGAAACGGTGGTGC+CGG - chr2.4:56077037-56077056 MS.gene059037:CDS 55.0%
!! TGGTGGACTTCCAGACACAG+TGG - chr2.4:56077446-56077465 MS.gene059037:intron 55.0%
GGTGGCAAAGCTAGGGGTGA+TGG - chr2.4:56077585-56077604 MS.gene059037:CDS 60.0%
TTGTCTGCAGGGCCAGATGG+AGG - chr2.4:56077492-56077511 MS.gene059037:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 56076908 56077656 56076908 ID=MS.gene059037
chr2.4 mRNA 56076908 56077656 56076908 ID=MS.gene059037.t1;Parent=MS.gene059037
chr2.4 exon 56077476 56077656 56077476 ID=MS.gene059037.t1.exon1;Parent=MS.gene059037.t1
chr2.4 CDS 56077476 56077656 56077476 ID=cds.MS.gene059037.t1;Parent=MS.gene059037.t1
chr2.4 exon 56076908 56077200 56076908 ID=MS.gene059037.t1.exon2;Parent=MS.gene059037.t1
chr2.4 CDS 56076908 56077200 56076908 ID=cds.MS.gene059037.t1;Parent=MS.gene059037.t1
Gene Sequence

>MS.gene059037

ATGGGTGTATTCAACTTTGAGGATGAGACCACATCTATTGTAGCTCCTGCTAGACTTTACAAAGCTCTAGTTACAGATTCTGATAACCTTATTCCAAAGGTTATTGATGCCATCCAAAGTATTGAAATTGTTGAAGGAAACGGTGGTGCCGGAACCATCAAGAAACTTACTTTCGTTGAAGGTTAGTATATATATGTGAAAAATTGAAGTGGCAGGAAACTTTTTTTAACCATCAAATGTTTGATTCTTGTTGATTGTGAAGTGCATATCCTCTTTTCTTTTATTATATATTGGATTATTTGTTACTATGTTTGTTATATAATATTTTTTTATGAGAAACAATAGTATTTTTAATTTTTTTTTTTAATAGCTTCTTATACAAATTATTAATAACCTAGTTGAACCAAGTGAATGTCCATCATGTTAGTAATTGTGAAACGAATCAATTATGTGCAGGTGGTGAAACAAAGTATGATTTGCACAAAGTGGACTTAGTAGATGATGTTAACTTCGCTTACAACTACAGCATAGTTGGTGGTGGTGGACTTCCAGACACAGTGGAGAAGATCTCCTTTGAGTCTAAATTGTCTGCAGGGCCAGATGGAGGATCCATTGCAAAGCTTACTGTGAAATACTTCACAAAAGGTGATGCTGCACCAAGTGAAGAGGAAATTAAGGGTGGCAAAGCTAGGGGTGATGGTCTTTTCAAGGCTCTTGAAGGTTACGTTTTGGCAAATCCTGATTACTAA

Protein sequence

>MS.gene059037.t1

MGVFNFEDETTSIVAPARLYKALVTDSDNLIPKVIDAIQSIEIVEGNGGAGTIKKLTFVEGGETKYDLHKVDLVDDVNFAYNYSIVGGGGLPDTVEKISFESKLSAGPDGGSIAKLTVKYFTKGDAAPSEEEIKGGKARGDGLFKALEGYVLANPDY