AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene79714


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79714.t1 MTR_8g023960 95.885 243 7 1 1 243 57 296 4.50e-168 465
MS.gene79714.t1 MTR_8g023960 95.885 243 7 1 1 243 1 240 1.18e-167 462
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene79714.t1 AT4G05400 55.686 255 96 5 1 243 1 250 6.96e-87 258
MS.gene79714.t1 AT4G05400 55.686 255 96 5 1 243 1 250 6.96e-87 258
MS.gene79714.t1 AT4G05400 55.686 255 96 5 1 243 1 250 6.96e-87 258
MS.gene79714.t1 AT4G21140 54.251 247 105 5 1 243 1 243 1.29e-73 224

Find 77 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTCTCAGAAACCCTAAATT+TGG 0.204627 8.4:+67786350 None:intergenic
ACCAGTTGGGGCACCGGGTG+TGG 0.216920 8.4:+67786542 None:intergenic
GAAGCAGAACGCGAGAAATT+AGG 0.227222 8.4:-67786426 MS.gene79714:CDS
GCCGGATTTGACTCCGTTTC+CGG 0.238916 8.4:-67786094 MS.gene79714:CDS
GTTTCCGGTGAATATGTTTA+TGG 0.240627 8.4:-67786079 MS.gene79714:CDS
TGCTTCGCGTGCGCGTTGTT+TGG 0.263470 8.4:+67786443 None:intergenic
GGGTTGGATTATATAACATT+AGG 0.279672 8.4:-67786306 MS.gene79714:CDS
TCTTTCGTCAATTCATATTT+CGG 0.289163 8.4:+67786261 None:intergenic
TTCTGAGAAGCCAACGATAA+TGG 0.299403 8.4:-67786337 MS.gene79714:CDS
TGGGGCACCGGGTGTGGGTT+CGG 0.342298 8.4:+67786548 None:intergenic
AAGCAGAACGCGAGAAATTA+GGG 0.344239 8.4:-67786425 MS.gene79714:CDS
GCTTCGCGTGCGCGTTGTTT+GGG 0.349445 8.4:+67786444 None:intergenic
GGTTGGATTATATAACATTA+GGG 0.357133 8.4:-67786305 MS.gene79714:CDS
GAATCTATTGAGGATGAAAA+AGG 0.364273 8.4:-67786163 MS.gene79714:CDS
GTTTCTCGCGGGAACCAGTT+GGG 0.371311 8.4:+67786529 None:intergenic
TCAGTGGGAAGGAGAAGATT+AGG 0.382186 8.4:-67785996 MS.gene79714:CDS
GAAAGAAGATGGGATGAAAT+TGG 0.382332 8.4:-67786244 MS.gene79714:CDS
AGGTTTGAAGGAAGCTGCTT+TGG 0.388226 8.4:-67786118 MS.gene79714:CDS
ACCGGGTGTGGGTTCGGATC+CGG 0.397530 8.4:+67786554 None:intergenic
TCTGAGAAGCCAACGATAAT+GGG 0.401787 8.4:-67786336 MS.gene79714:CDS
CCCACACCCGGTGCCCCAAC+TGG 0.402280 8.4:-67786543 MS.gene79714:CDS
TTATATAACATTAGGGTTAG+TGG 0.413282 8.4:-67786298 MS.gene79714:CDS
AGCCGCACCGCAAAAGGAAA+AGG 0.413446 8.4:-67786624 MS.gene79714:CDS
GCGGGAACCAGTTGGGGCAC+CGG 0.419435 8.4:+67786536 None:intergenic
TCCGTCAGCCGCACCGCAAA+AGG 0.432750 8.4:-67786630 MS.gene79714:CDS
GAGGCTATTGAAGCTTTGCC+TGG 0.437917 8.4:-67786141 MS.gene79714:CDS
CGGGAACCAGTTGGGGCACC+GGG 0.441391 8.4:+67786537 None:intergenic
TATGAATTGACGAAAGAAGA+TGG 0.442226 8.4:-67786255 MS.gene79714:CDS
TGGGTATATTGAGAAGATTA+AGG 0.447659 8.4:-67786034 MS.gene79714:CDS
AGCTTTGCCTGGAGGTTTGA+AGG 0.449486 8.4:-67786130 MS.gene79714:CDS
CCAGTTGGGGCACCGGGTGT+GGG 0.450669 8.4:+67786543 None:intergenic
TGTTTCTCGCGGGAACCAGT+TGG 0.454283 8.4:+67786528 None:intergenic
CGGAGCAGCGTTGGAGGAGT+TGG 0.459479 8.4:+67786649 None:intergenic
TGCGGCTGACGGAGCAGCGT+TGG 0.460867 8.4:+67786640 None:intergenic
AGATTAAGGAGGCTGCTGAT+AGG 0.463125 8.4:-67786020 MS.gene79714:CDS
ACGCTTACACCGCCTATTGA+TGG 0.467403 8.4:-67786054 MS.gene79714:CDS
TTCTCAATATACCCATCAAT+AGG 0.469115 8.4:+67786042 None:intergenic
GAAGGAAGCTGCTTTGGTGC+CGG 0.473891 8.4:-67786112 MS.gene79714:CDS
CGCTTACACCGCCTATTGAT+GGG 0.481678 8.4:-67786053 MS.gene79714:CDS
GGCTGACGGAGCAGCGTTGG+AGG 0.488541 8.4:+67786643 None:intergenic
ACCGGAAACGGAGTCAAATC+CGG 0.495763 8.4:+67786093 None:intergenic
ATAAACATATTCACCGGAAA+CGG 0.497141 8.4:+67786081 None:intergenic
CCAACGATAATGGGAACACC+TGG 0.497189 8.4:-67786327 MS.gene79714:CDS
CCAGGTGTTCCCATTATCGT+TGG 0.510994 8.4:+67786327 None:intergenic
TTCGCGTTCTTGTTTCTCGC+GGG 0.513895 8.4:+67786518 None:intergenic
ACCGGATCCGAACCCACACC+CGG 0.521607 8.4:-67786555 MS.gene79714:CDS
ATGAATTGACGAAAGAAGAT+GGG 0.521993 8.4:-67786254 MS.gene79714:CDS
GCAGCTTCCTTCAAACCTCC+AGG 0.522912 8.4:+67786123 None:intergenic
TTTCTCGCGGGAACCAGTTG+GGG 0.524113 8.4:+67786530 None:intergenic
AATTGGCGAAGGAGTATAGC+AGG 0.524252 8.4:-67786227 MS.gene79714:CDS
GCCGCACCGCAAAAGGAAAA+GGG 0.525322 8.4:-67786623 MS.gene79714:CDS
GATAATGGGAACACCTGGGT+TGG 0.543156 8.4:-67786322 MS.gene79714:CDS
GTTCGCGTTCTTGTTTCTCG+CGG 0.547502 8.4:+67786517 None:intergenic
GCAAAAGGAAAAGGGAAAGC+CGG 0.550247 8.4:-67786615 MS.gene79714:CDS
TTGAGAAAACGAAGTGGTGT+TGG 0.571375 8.4:+67786468 None:intergenic
CGGCGGAGACGAATCTATTG+AGG 0.575732 8.4:-67786173 MS.gene79714:CDS
ATCAATAGGCGGTGTAAGCG+TGG 0.581049 8.4:+67786056 None:intergenic
AGATGGGATGAAATTGGCGA+AGG 0.597418 8.4:-67786238 MS.gene79714:CDS
GAAGCATAGGGCGAGACAAG+CGG 0.597439 8.4:-67786193 MS.gene79714:CDS
GCTATTGAAGCTTTGCCTGG+AGG 0.598696 8.4:-67786138 MS.gene79714:CDS
TGCTGATAGGATCAGTGGGA+AGG 0.607298 8.4:-67786007 MS.gene79714:CDS
GGTGAAGATGACAGTGGTGA+TGG 0.610256 8.4:+67786675 None:intergenic
GAGGCTGCTGATAGGATCAG+TGG 0.610487 8.4:-67786012 MS.gene79714:CDS
AGGCTGCTGATAGGATCAGT+GGG 0.618985 8.4:-67786011 MS.gene79714:CDS
GTGGCCATAAACATATTCAC+CGG 0.619972 8.4:+67786075 None:intergenic
CAGCGTTGGAGGAGTTGGTG+CGG 0.641635 8.4:+67786654 None:intergenic
GCATAGGGCGAGACAAGCGG+CGG 0.658080 8.4:-67786190 MS.gene79714:CDS
ATTGGCGAAGGAGTATAGCA+GGG 0.662162 8.4:-67786226 MS.gene79714:CDS
CGTTGAGGCATTGATCGTAG+AGG 0.672109 8.4:+67786493 None:intergenic
GAAGATGACAGTGGTGATGG+TGG 0.683610 8.4:+67786678 None:intergenic
GTATATTGAGAAGATTAAGG+AGG 0.685736 8.4:-67786031 MS.gene79714:CDS
TCAATATACCCATCAATAGG+CGG 0.685982 8.4:+67786045 None:intergenic
AATGTTATATAATCCAACCC+AGG 0.686284 8.4:+67786309 None:intergenic
CAACGATAATGGGAACACCT+GGG 0.706454 8.4:-67786326 MS.gene79714:CDS
TTGGGTTTGAGAAAACGAAG+TGG 0.743892 8.4:+67786462 None:intergenic
GAAGTGGTGTTGGTGCGTTG+AGG 0.759588 8.4:+67786478 None:intergenic
TGGTGCGGTGAAGATGACAG+TGG 0.785449 8.4:+67786669 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAATCACTGTCAAAAAAAC+CGG - chr8.4:67786122-67786141 MS.gene79714:CDS 25.0%
! GGTTGGATTATATAACATTA+GGG - chr8.4:67786390-67786409 MS.gene79714:CDS 25.0%
! TCTTTCGTCAATTCATATTT+CGG + chr8.4:67786437-67786456 None:intergenic 25.0%
! TTATATAACATTAGGGTTAG+TGG - chr8.4:67786397-67786416 MS.gene79714:CDS 25.0%
AATGTTATATAATCCAACCC+AGG + chr8.4:67786389-67786408 None:intergenic 30.0%
AGAAGAAAAACGCCAAATTT+AGG - chr8.4:67786333-67786352 MS.gene79714:CDS 30.0%
ATAAACATATTCACCGGAAA+CGG + chr8.4:67786617-67786636 None:intergenic 30.0%
ATGAATTGACGAAAGAAGAT+GGG - chr8.4:67786441-67786460 MS.gene79714:CDS 30.0%
GAAGAAAAACGCCAAATTTA+GGG - chr8.4:67786334-67786353 MS.gene79714:CDS 30.0%
GAATCTATTGAGGATGAAAA+AGG - chr8.4:67786532-67786551 MS.gene79714:CDS 30.0%
GGGTTGGATTATATAACATT+AGG - chr8.4:67786389-67786408 MS.gene79714:CDS 30.0%
GTATATTGAGAAGATTAAGG+AGG - chr8.4:67786664-67786683 MS.gene79714:CDS 30.0%
TATGAATTGACGAAAGAAGA+TGG - chr8.4:67786440-67786459 MS.gene79714:CDS 30.0%
TGGGTATATTGAGAAGATTA+AGG - chr8.4:67786661-67786680 MS.gene79714:CDS 30.0%
TTCTCAATATACCCATCAAT+AGG + chr8.4:67786656-67786675 None:intergenic 30.0%
!!! GGTTTTTTTGACAGTGATTT+TGG + chr8.4:67786123-67786142 None:intergenic 30.0%
CTTCTCAGAAACCCTAAATT+TGG + chr8.4:67786348-67786367 None:intergenic 35.0%
GAAAGAAGATGGGATGAAAT+TGG - chr8.4:67786451-67786470 MS.gene79714:CDS 35.0%
GTTTCCGGTGAATATGTTTA+TGG - chr8.4:67786616-67786635 MS.gene79714:CDS 35.0%
TCAATATACCCATCAATAGG+CGG + chr8.4:67786653-67786672 None:intergenic 35.0%
TCTATTGAGGATGAAAAAGG+AGG - chr8.4:67786535-67786554 MS.gene79714:CDS 35.0%
!!! CAGTGATTTTGGAACGTTTT+AGG + chr8.4:67786112-67786131 None:intergenic 35.0%
AAGCAGAACGCGAGAAATTA+GGG - chr8.4:67786270-67786289 MS.gene79714:CDS 40.0%
GTGGCCATAAACATATTCAC+CGG + chr8.4:67786623-67786642 None:intergenic 40.0%
TCTGAGAAGCCAACGATAAT+GGG - chr8.4:67786359-67786378 MS.gene79714:CDS 40.0%
TTGAGAAAACGAAGTGGTGT+TGG + chr8.4:67786230-67786249 None:intergenic 40.0%
TTGGGTTTGAGAAAACGAAG+TGG + chr8.4:67786236-67786255 None:intergenic 40.0%
! TTCTGAGAAGCCAACGATAA+TGG - chr8.4:67786358-67786377 MS.gene79714:CDS 40.0%
!! AGTATAGCAGGGTTTTGATG+AGG - chr8.4:67786480-67786499 MS.gene79714:CDS 40.0%
!! GGTTTTGATGAGGAAGCATA+GGG - chr8.4:67786490-67786509 MS.gene79714:CDS 40.0%
!!! ATGGTGGTTTTAGCTTTGTG+AGG + chr8.4:67786004-67786023 None:intergenic 40.0%
!!! GGAACGTTTTAGGATTTGAC+CGG + chr8.4:67786102-67786121 None:intergenic 40.0%
AGATGGGATGAAATTGGCGA+AGG - chr8.4:67786457-67786476 MS.gene79714:CDS 45.0%
AGATTAAGGAGGCTGCTGAT+AGG - chr8.4:67786675-67786694 MS.gene79714:CDS 45.0%
AGGTTTGAAGGAAGCTGCTT+TGG - chr8.4:67786577-67786596 MS.gene79714:CDS 45.0%
GAAGCAGAACGCGAGAAATT+AGG - chr8.4:67786269-67786288 MS.gene79714:CDS 45.0%
GCAAAAGGAAAAGGGAAAGC+CGG - chr8.4:67786080-67786099 MS.gene79714:CDS 45.0%
TCAGTGGGAAGGAGAAGATT+AGG - chr8.4:67786699-67786718 MS.gene79714:CDS 45.0%
! AATTGGCGAAGGAGTATAGC+AGG - chr8.4:67786468-67786487 MS.gene79714:CDS 45.0%
! ATTGGCGAAGGAGTATAGCA+GGG - chr8.4:67786469-67786488 MS.gene79714:CDS 45.0%
! GGCTTTCCCTTTTCCTTTTG+CGG + chr8.4:67786081-67786100 None:intergenic 45.0%
!! CAACGATAATGGGAACACCT+GGG - chr8.4:67786369-67786388 MS.gene79714:CDS 45.0%
!! GGGTTTTGATGAGGAAGCAT+AGG - chr8.4:67786489-67786508 MS.gene79714:CDS 45.0%
ACCGGAAACGGAGTCAAATC+CGG + chr8.4:67786605-67786624 None:intergenic 50.0%
ACGCTTACACCGCCTATTGA+TGG - chr8.4:67786641-67786660 MS.gene79714:CDS 50.0%
AGCTTTGCCTGGAGGTTTGA+AGG - chr8.4:67786565-67786584 MS.gene79714:CDS 50.0%
AGGCTGCTGATAGGATCAGT+GGG - chr8.4:67786684-67786703 MS.gene79714:CDS 50.0%
ATCAATAGGCGGTGTAAGCG+TGG + chr8.4:67786642-67786661 None:intergenic 50.0%
CCAGGTGTTCCCATTATCGT+TGG + chr8.4:67786371-67786390 None:intergenic 50.0%
CGCTTACACCGCCTATTGAT+GGG - chr8.4:67786642-67786661 MS.gene79714:CDS 50.0%
GAGGCTATTGAAGCTTTGCC+TGG - chr8.4:67786554-67786573 MS.gene79714:CDS 50.0%
GCTATTGAAGCTTTGCCTGG+AGG - chr8.4:67786557-67786576 MS.gene79714:CDS 50.0%
GGTGAAGATGACAGTGGTGA+TGG + chr8.4:67786023-67786042 None:intergenic 50.0%
TGCTGATAGGATCAGTGGGA+AGG - chr8.4:67786688-67786707 MS.gene79714:CDS 50.0%
! GTTCGCGTTCTTGTTTCTCG+CGG + chr8.4:67786181-67786200 None:intergenic 50.0%
! TCCCTTTTCCTTTTGCGGTG+CGG + chr8.4:67786076-67786095 None:intergenic 50.0%
! TTCGCGTTCTTGTTTCTCGC+GGG + chr8.4:67786180-67786199 None:intergenic 50.0%
!! CCAACGATAATGGGAACACC+TGG - chr8.4:67786368-67786387 MS.gene79714:CDS 50.0%
!! CGTTGAGGCATTGATCGTAG+AGG + chr8.4:67786205-67786224 None:intergenic 50.0%
!! GAAGATGACAGTGGTGATGG+TGG + chr8.4:67786020-67786039 None:intergenic 50.0%
!! GATAATGGGAACACCTGGGT+TGG - chr8.4:67786373-67786392 MS.gene79714:CDS 50.0%
AGCCGCACCGCAAAAGGAAA+AGG - chr8.4:67786071-67786090 MS.gene79714:CDS 55.0%
CGGCGGAGACGAATCTATTG+AGG - chr8.4:67786522-67786541 MS.gene79714:CDS 55.0%
GAAGCATAGGGCGAGACAAG+CGG - chr8.4:67786502-67786521 MS.gene79714:CDS 55.0%
GAGGCTGCTGATAGGATCAG+TGG - chr8.4:67786683-67786702 MS.gene79714:CDS 55.0%
GCAGCTTCCTTCAAACCTCC+AGG + chr8.4:67786575-67786594 None:intergenic 55.0%
GCCGCACCGCAAAAGGAAAA+GGG - chr8.4:67786072-67786091 MS.gene79714:CDS 55.0%
GCCGGATTTGACTCCGTTTC+CGG - chr8.4:67786601-67786620 MS.gene79714:CDS 55.0%
GTTTCTCGCGGGAACCAGTT+GGG + chr8.4:67786169-67786188 None:intergenic 55.0%
TGGTGCGGTGAAGATGACAG+TGG + chr8.4:67786029-67786048 None:intergenic 55.0%
TGTTTCTCGCGGGAACCAGT+TGG + chr8.4:67786170-67786189 None:intergenic 55.0%
TTTCTCGCGGGAACCAGTTG+GGG + chr8.4:67786168-67786187 None:intergenic 55.0%
! GAAGGAAGCTGCTTTGGTGC+CGG - chr8.4:67786583-67786602 MS.gene79714:CDS 55.0%
!! GAAGTGGTGTTGGTGCGTTG+AGG + chr8.4:67786220-67786239 None:intergenic 55.0%
GCTTCGCGTGCGCGTTGTTT+GGG + chr8.4:67786254-67786273 None:intergenic 60.0%
TGCTTCGCGTGCGCGTTGTT+TGG + chr8.4:67786255-67786274 None:intergenic 60.0%
! TCCTTTTGCGGTGCGGCTGA+CGG + chr8.4:67786069-67786088 None:intergenic 60.0%
!! CAGCGTTGGAGGAGTTGGTG+CGG + chr8.4:67786044-67786063 None:intergenic 60.0%
ACCGGATCCGAACCCACACC+CGG - chr8.4:67786140-67786159 MS.gene79714:CDS 65.0%
ACCGGGTGTGGGTTCGGATC+CGG + chr8.4:67786144-67786163 None:intergenic 65.0%
GCATAGGGCGAGACAAGCGG+CGG - chr8.4:67786505-67786524 MS.gene79714:CDS 65.0%
TCCGTCAGCCGCACCGCAAA+AGG - chr8.4:67786065-67786084 MS.gene79714:CDS 65.0%
! CGGAGCAGCGTTGGAGGAGT+TGG + chr8.4:67786049-67786068 None:intergenic 65.0%
GCGGGAACCAGTTGGGGCAC+CGG + chr8.4:67786162-67786181 None:intergenic 70.0%
TGCGGCTGACGGAGCAGCGT+TGG + chr8.4:67786058-67786077 None:intergenic 70.0%
! CGGGAACCAGTTGGGGCACC+GGG + chr8.4:67786161-67786180 None:intergenic 70.0%
! GGCTGACGGAGCAGCGTTGG+AGG + chr8.4:67786055-67786074 None:intergenic 70.0%
!! ACCAGTTGGGGCACCGGGTG+TGG + chr8.4:67786156-67786175 None:intergenic 70.0%
!! CCAGTTGGGGCACCGGGTGT+GGG + chr8.4:67786155-67786174 None:intergenic 70.0%
!! TGGGGCACCGGGTGTGGGTT+CGG + chr8.4:67786150-67786169 None:intergenic 70.0%
CCCACACCCGGTGCCCCAAC+TGG - chr8.4:67786152-67786171 MS.gene79714:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 67785993 67786724 67785993 ID=MS.gene79714
chr8.4 mRNA 67785993 67786724 67785993 ID=MS.gene79714.t1;Parent=MS.gene79714
chr8.4 exon 67785993 67786724 67785993 ID=MS.gene79714.t1.exon1;Parent=MS.gene79714.t1
chr8.4 CDS 67785993 67786724 67785993 ID=cds.MS.gene79714.t1;Parent=MS.gene79714.t1
Gene Sequence

>MS.gene79714

ATGCTTCGCCTCACAAAGCTAAAACCACCATCACCACTGTCATCTTCACCGCACCAACTCCTCCAACGCTGCTCCGTCAGCCGCACCGCAAAAGGAAAAGGGAAAGCCGGTCAAATCCTAAAACGTTCCAAAATCACTGTCAAAAAAACCGGATCCGAACCCACACCCGGTGCCCCAACTGGTTCCCGCGAGAAACAAGAACGCGAACGCCTCTACGATCAATGCCTCAACGCACCAACACCACTTCGTTTTCTCAAACCCAAACAACGCGCACGCGAAGCAGAACGCGAGAAATTAGGGTTAATTAGCAAAGATCGTCAAATTGAACTCGATATGATGAAGAAGAAAAACGCCAAATTTAGGGTTTCTGAGAAGCCAACGATAATGGGAACACCTGGGTTGGATTATATAACATTAGGGTTAGTGGATGCTGAGAAATTGCCGAAATATGAATTGACGAAAGAAGATGGGATGAAATTGGCGAAGGAGTATAGCAGGGTTTTGATGAGGAAGCATAGGGCGAGACAAGCGGCGGAGACGAATCTATTGAGGATGAAAAAGGAGGCTATTGAAGCTTTGCCTGGAGGTTTGAAGGAAGCTGCTTTGGTGCCGGATTTGACTCCGTTTCCGGTGAATATGTTTATGGCCACGCTTACACCGCCTATTGATGGGTATATTGAGAAGATTAAGGAGGCTGCTGATAGGATCAGTGGGAAGGAGAAGATTAGGTAG

Protein sequence

>MS.gene79714.t1

MLRLTKLKPPSPLSSSPHQLLQRCSVSRTAKGKGKAGQILKRSKITVKKTGSEPTPGAPTGSREKQERERLYDQCLNAPTPLRFLKPKQRAREAEREKLGLISKDRQIELDMMKKKNAKFRVSEKPTIMGTPGLDYITLGLVDAEKLPKYELTKEDGMKLAKEYSRVLMRKHRARQAAETNLLRMKKEAIEALPGGLKEAALVPDLTPFPVNMFMATLTPPIDGYIEKIKEAADRISGKEKIR