AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene92136


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92136.t1 MTR_8g023960 95.473 243 8 1 1 243 57 296 4.45e-167 463
MS.gene92136.t1 MTR_8g023960 95.473 243 8 1 1 243 1 240 3.24e-166 458
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene92136.t1 AT4G05400 56.078 255 95 5 1 243 1 250 8.19e-88 260
MS.gene92136.t1 AT4G05400 56.078 255 95 5 1 243 1 250 8.19e-88 260
MS.gene92136.t1 AT4G05400 56.078 255 95 5 1 243 1 250 8.19e-88 260
MS.gene92136.t1 AT4G21140 54.656 247 104 5 1 243 1 243 1.06e-74 227

Find 75 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAGTTGGGGCACCGGGTG+TGG 0.216920 8.3:+65480216 None:intergenic
TCCGGATTTGACTCCGTTTC+CGG 0.238916 8.3:-65479768 MS.gene92136:CDS
GTTTCCGGTGAATAGGTTTA+TGG 0.264318 8.3:-65479753 MS.gene92136:CDS
TCTTTCGTCAATTCATATTT+CGG 0.289163 8.3:+65479935 None:intergenic
AGCTTCGCGTGCACGTTGTT+TGG 0.290006 8.3:+65480117 None:intergenic
TTCTGAGAAGCCAACGATAA+TGG 0.299403 8.3:-65480011 MS.gene92136:CDS
TGGGGCACCGGGTGTGGGTT+CGG 0.342298 8.3:+65480222 None:intergenic
GAAGGAAGCTGCTTTGGTTC+CGG 0.344771 8.3:-65479786 MS.gene92136:CDS
GCTTCGCGTGCACGTTGTTT+GGG 0.366189 8.3:+65480118 None:intergenic
GGTTTGGATTATATAACGTT+AGG 0.370627 8.3:-65479980 MS.gene92136:CDS
GTTTCTCGCGGGAACCAGTT+GGG 0.371311 8.3:+65480203 None:intergenic
GATAATGGGAACACCTGGTT+TGG 0.375812 8.3:-65479996 MS.gene92136:CDS
TCAGTGGGAAGGAGAAGATT+AGG 0.382186 8.3:-65479670 MS.gene92136:CDS
GAAAGAAGATGGGATGAAAT+TGG 0.382332 8.3:-65479918 MS.gene92136:CDS
TTATATAACGTTAGGGTTAG+TGG 0.388954 8.3:-65479972 MS.gene92136:CDS
TGACTCCGTTTCCGGTGAAT+AGG 0.396696 8.3:-65479760 MS.gene92136:CDS
ACCGGGTGTGGGTTCGGATC+CGG 0.397530 8.3:+65480228 None:intergenic
TCTGAGAAGCCAACGATAAT+GGG 0.401787 8.3:-65480010 MS.gene92136:CDS
CCCACACCCGGTGCCCCAAC+TGG 0.402280 8.3:-65480217 MS.gene92136:CDS
AATTGGCGAAGGAGTATAGT+AGG 0.407802 8.3:-65479901 MS.gene92136:CDS
AGCCGCACCGCAAAAGGAAA+AGG 0.413446 8.3:-65480298 MS.gene92136:CDS
GGGTTTGAAGGAAGCTGCTT+TGG 0.416358 8.3:-65479792 MS.gene92136:CDS
GCGGGAACCAGTTGGGGCAC+CGG 0.419435 8.3:+65480210 None:intergenic
GGGGAGGATGACAGTGGTGA+TGG 0.423787 8.3:+65480349 None:intergenic
TCCGTCAGCCGCACCGCAAA+AGG 0.432750 8.3:-65480304 MS.gene92136:CDS
CGGGAACCAGTTGGGGCACC+GGG 0.441391 8.3:+65480211 None:intergenic
TATGAATTGACGAAAGAAGA+TGG 0.442226 8.3:-65479929 MS.gene92136:CDS
ACCGGAAACGGAGTCAAATC+CGG 0.446628 8.3:+65479767 None:intergenic
TGGGTATATTGAGAAGATTA+AGG 0.447659 8.3:-65479708 MS.gene92136:CDS
CCAGTTGGGGCACCGGGTGT+GGG 0.450669 8.3:+65480217 None:intergenic
TGTTTCTCGCGGGAACCAGT+TGG 0.454283 8.3:+65480202 None:intergenic
ATAAACCTATTCACCGGAAA+CGG 0.456384 8.3:+65479755 None:intergenic
TGCGGCTGACGGAGCAGCGT+TGG 0.460867 8.3:+65480314 None:intergenic
AGATTAAGGAGGCTGCTGAT+AGG 0.463125 8.3:-65479694 MS.gene92136:CDS
CGGAGCAGCGTTGGAGGAGT+TGG 0.464939 8.3:+65480323 None:intergenic
GTTTGGATTATATAACGTTA+GGG 0.470062 8.3:-65479979 MS.gene92136:CDS
TTCTCAATATACCCATCAAT+AGG 0.473537 8.3:+65479716 None:intergenic
AGCGTTGGAGGAGTTGGTGT+GGG 0.476604 8.3:+65480329 None:intergenic
AGCAGCTTCCTTCAAACCCC+CGG 0.485024 8.3:+65479796 None:intergenic
GGCTGACGGAGCAGCGTTGG+AGG 0.488541 8.3:+65480317 None:intergenic
GAGGATGACAGTGGTGATGG+TGG 0.488777 8.3:+65480352 None:intergenic
ACGCTTACGCCACCTATTGA+TGG 0.498561 8.3:-65479728 MS.gene92136:CDS
TTCGCGTTCTTGTTTCTCGC+GGG 0.513895 8.3:+65480192 None:intergenic
GAGGCTATTGAAGCTTTACC+CGG 0.517897 8.3:-65479815 MS.gene92136:CDS
CAGCGTTGGAGGAGTTGGTG+TGG 0.521072 8.3:+65480328 None:intergenic
ACCGGATCCGAACCCACACC+CGG 0.521607 8.3:-65480229 MS.gene92136:CDS
ATGAATTGACGAAAGAAGAT+GGG 0.521993 8.3:-65479928 MS.gene92136:CDS
TTTCTCGCGGGAACCAGTTG+GGG 0.524113 8.3:+65480204 None:intergenic
GCCGCACCGCAAAAGGAAAA+GGG 0.525322 8.3:-65480297 MS.gene92136:CDS
CCAGGTGTTCCCATTATCGT+TGG 0.527921 8.3:+65480001 None:intergenic
CATCCTCAAAAGATTCGTCT+CGG 0.529672 8.3:+65479844 None:intergenic
TTGGAGGAGTTGGTGTGGGG+AGG 0.529803 8.3:+65480333 None:intergenic
CGCTTACGCCACCTATTGAT+GGG 0.534577 8.3:-65479727 MS.gene92136:CDS
GCAAAAGGAAAAGGGAAAGC+TGG 0.540848 8.3:-65480289 MS.gene92136:CDS
GTTCGCGTTCTTGTTTCTCG+CGG 0.547502 8.3:+65480191 None:intergenic
ATTGGCGAAGGAGTATAGTA+GGG 0.549849 8.3:-65479900 MS.gene92136:CDS
TTGAGAAAACGAAGTGGTGT+TGG 0.571375 8.3:+65480142 None:intergenic
GCGTTGGAGGAGTTGGTGTG+GGG 0.585225 8.3:+65480330 None:intergenic
CCAACGATAATGGGAACACC+TGG 0.593653 8.3:-65480001 MS.gene92136:CDS
AGATGGGATGAAATTGGCGA+AGG 0.597418 8.3:-65479912 MS.gene92136:CDS
GAAGCATAGGGCGAGACAAG+CGG 0.597439 8.3:-65479867 MS.gene92136:CDS
TGCTGATAGGATCAGTGGGA+AGG 0.607298 8.3:-65479681 MS.gene92136:CDS
GAGGCTGCTGATAGGATCAG+TGG 0.610487 8.3:-65479686 MS.gene92136:CDS
GTCGCCATAAACCTATTCAC+CGG 0.613259 8.3:+65479749 None:intergenic
GGCTATTGAAGCTTTACCCG+GGG 0.615693 8.3:-65479813 MS.gene92136:CDS
AGGCTGCTGATAGGATCAGT+GGG 0.618985 8.3:-65479685 MS.gene92136:CDS
AGGCTATTGAAGCTTTACCC+GGG 0.626565 8.3:-65479814 MS.gene92136:CDS
TGGTGTGGGGAGGATGACAG+TGG 0.627178 8.3:+65480343 None:intergenic
TCAATATACCCATCAATAGG+TGG 0.649441 8.3:+65479719 None:intergenic
CGTTGAGGCATTGATCGTAG+AGG 0.672109 8.3:+65480167 None:intergenic
AACGTTATATAATCCAAACC+AGG 0.673172 8.3:+65479983 None:intergenic
GTATATTGAGAAGATTAAGG+AGG 0.685736 8.3:-65479705 MS.gene92136:CDS
GCTATTGAAGCTTTACCCGG+GGG 0.716608 8.3:-65479812 MS.gene92136:CDS
TTGGGTTTGAGAAAACGAAG+TGG 0.743892 8.3:+65480136 None:intergenic
GAAGTGGTGTTGGTGCGTTG+AGG 0.759588 8.3:+65480152 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAATCACTGTCAAAAAAAC+CGG - chr8.3:65479796-65479815 MS.gene92136:CDS 25.0%
! GTTTGGATTATATAACGTTA+GGG - chr8.3:65480064-65480083 MS.gene92136:CDS 25.0%
! TCTTTCGTCAATTCATATTT+CGG + chr8.3:65480111-65480130 None:intergenic 25.0%
AACGTTATATAATCCAAACC+AGG + chr8.3:65480063-65480082 None:intergenic 30.0%
AAGCTGAACGAGAAAAATTA+GGG - chr8.3:65479944-65479963 MS.gene92136:CDS 30.0%
ATGAATTGACGAAAGAAGAT+GGG - chr8.3:65480115-65480134 MS.gene92136:CDS 30.0%
GGTTTGGATTATATAACGTT+AGG - chr8.3:65480063-65480082 MS.gene92136:CDS 30.0%
GTATATTGAGAAGATTAAGG+AGG - chr8.3:65480338-65480357 MS.gene92136:CDS 30.0%
TATGAATTGACGAAAGAAGA+TGG - chr8.3:65480114-65480133 MS.gene92136:CDS 30.0%
TGGGTATATTGAGAAGATTA+AGG - chr8.3:65480335-65480354 MS.gene92136:CDS 30.0%
TTATATAACGTTAGGGTTAG+TGG - chr8.3:65480071-65480090 MS.gene92136:CDS 30.0%
TTCTCAATATACCCATCAAT+AGG + chr8.3:65480330-65480349 None:intergenic 30.0%
!! GAATCTTTTGAGGATGAAAA+AGG - chr8.3:65480206-65480225 MS.gene92136:CDS 30.0%
!!! GGTTTTTTTGACAGTGATTT+TGG + chr8.3:65479797-65479816 None:intergenic 30.0%
AGAAGAAAAACGCGAAGTTT+AGG - chr8.3:65480007-65480026 MS.gene92136:CDS 35.0%
ATAAACCTATTCACCGGAAA+CGG + chr8.3:65480291-65480310 None:intergenic 35.0%
GAAAGAAGATGGGATGAAAT+TGG - chr8.3:65480125-65480144 MS.gene92136:CDS 35.0%
GAAGAAAAACGCGAAGTTTA+GGG - chr8.3:65480008-65480027 MS.gene92136:CDS 35.0%
GAAGCTGAACGAGAAAAATT+AGG - chr8.3:65479943-65479962 MS.gene92136:CDS 35.0%
TCAATATACCCATCAATAGG+TGG + chr8.3:65480327-65480346 None:intergenic 35.0%
!! AGTATAGTAGGGTTTTGATG+AGG - chr8.3:65480154-65480173 MS.gene92136:CDS 35.0%
!! TCTTTTGAGGATGAAAAAGG+AGG - chr8.3:65480209-65480228 MS.gene92136:CDS 35.0%
!!! CAGTGATTTTGGAACGTTTT+AGG + chr8.3:65479786-65479805 None:intergenic 35.0%
CATCCTCAAAAGATTCGTCT+CGG + chr8.3:65480202-65480221 None:intergenic 40.0%
GTTTCCGGTGAATAGGTTTA+TGG - chr8.3:65480290-65480309 MS.gene92136:CDS 40.0%
TCTGAGAAGCCAACGATAAT+GGG - chr8.3:65480033-65480052 MS.gene92136:CDS 40.0%
TTGAGAAAACGAAGTGGTGT+TGG + chr8.3:65479904-65479923 None:intergenic 40.0%
TTGGGTTTGAGAAAACGAAG+TGG + chr8.3:65479910-65479929 None:intergenic 40.0%
! AATTGGCGAAGGAGTATAGT+AGG - chr8.3:65480142-65480161 MS.gene92136:CDS 40.0%
! AGCTTTCCCTTTTCCTTTTG+CGG + chr8.3:65479755-65479774 None:intergenic 40.0%
! ATTGGCGAAGGAGTATAGTA+GGG - chr8.3:65480143-65480162 MS.gene92136:CDS 40.0%
! TTCTGAGAAGCCAACGATAA+TGG - chr8.3:65480032-65480051 MS.gene92136:CDS 40.0%
!! GGTTTTGATGAGGAAGCATA+GGG - chr8.3:65480164-65480183 MS.gene92136:CDS 40.0%
!!! ATGGTGGTTTTAGCTTTGTG+AGG + chr8.3:65479678-65479697 None:intergenic 40.0%
AGATGGGATGAAATTGGCGA+AGG - chr8.3:65480131-65480150 MS.gene92136:CDS 45.0%
AGATTAAGGAGGCTGCTGAT+AGG - chr8.3:65480349-65480368 MS.gene92136:CDS 45.0%
AGGCTATTGAAGCTTTACCC+GGG - chr8.3:65480229-65480248 MS.gene92136:CDS 45.0%
GAGGCTATTGAAGCTTTACC+CGG - chr8.3:65480228-65480247 MS.gene92136:CDS 45.0%
GCAAAAGGAAAAGGGAAAGC+TGG - chr8.3:65479754-65479773 MS.gene92136:CDS 45.0%
GTCGCCATAAACCTATTCAC+CGG + chr8.3:65480297-65480316 None:intergenic 45.0%
TCAGTGGGAAGGAGAAGATT+AGG - chr8.3:65480373-65480392 MS.gene92136:CDS 45.0%
!! GATAATGGGAACACCTGGTT+TGG - chr8.3:65480047-65480066 MS.gene92136:CDS 45.0%
!! GGGTTTTGATGAGGAAGCAT+AGG - chr8.3:65480163-65480182 MS.gene92136:CDS 45.0%
ACCGGAAACGGAGTCAAATC+CGG + chr8.3:65480279-65480298 None:intergenic 50.0%
ACGCTTACGCCACCTATTGA+TGG - chr8.3:65480315-65480334 MS.gene92136:CDS 50.0%
AGGCTGCTGATAGGATCAGT+GGG - chr8.3:65480358-65480377 MS.gene92136:CDS 50.0%
CCAGGTGTTCCCATTATCGT+TGG + chr8.3:65480045-65480064 None:intergenic 50.0%
CGCTTACGCCACCTATTGAT+GGG - chr8.3:65480316-65480335 MS.gene92136:CDS 50.0%
GCTATTGAAGCTTTACCCGG+GGG - chr8.3:65480231-65480250 MS.gene92136:CDS 50.0%
GGCTATTGAAGCTTTACCCG+GGG - chr8.3:65480230-65480249 MS.gene92136:CDS 50.0%
GGGTTTGAAGGAAGCTGCTT+TGG - chr8.3:65480251-65480270 MS.gene92136:CDS 50.0%
TCCGGATTTGACTCCGTTTC+CGG - chr8.3:65480275-65480294 MS.gene92136:CDS 50.0%
TGACTCCGTTTCCGGTGAAT+AGG - chr8.3:65480283-65480302 MS.gene92136:CDS 50.0%
TGCTGATAGGATCAGTGGGA+AGG - chr8.3:65480362-65480381 MS.gene92136:CDS 50.0%
! GAAGGAAGCTGCTTTGGTTC+CGG - chr8.3:65480257-65480276 MS.gene92136:CDS 50.0%
! GTTCGCGTTCTTGTTTCTCG+CGG + chr8.3:65479855-65479874 None:intergenic 50.0%
! TCCCTTTTCCTTTTGCGGTG+CGG + chr8.3:65479750-65479769 None:intergenic 50.0%
! TTCGCGTTCTTGTTTCTCGC+GGG + chr8.3:65479854-65479873 None:intergenic 50.0%
!! CCAACGATAATGGGAACACC+TGG - chr8.3:65480042-65480061 MS.gene92136:CDS 50.0%
!! CGTTGAGGCATTGATCGTAG+AGG + chr8.3:65479879-65479898 None:intergenic 50.0%
AGCAGCTTCCTTCAAACCCC+CGG + chr8.3:65480250-65480269 None:intergenic 55.0%
AGCCGCACCGCAAAAGGAAA+AGG - chr8.3:65479745-65479764 MS.gene92136:CDS 55.0%
AGCTTCGCGTGCACGTTGTT+TGG + chr8.3:65479929-65479948 None:intergenic 55.0%
AGCTTTACCCGGGGGTTTGA+AGG - chr8.3:65480239-65480258 MS.gene92136:CDS 55.0%
GAAGCATAGGGCGAGACAAG+CGG - chr8.3:65480176-65480195 MS.gene92136:CDS 55.0%
GAGGCTGCTGATAGGATCAG+TGG - chr8.3:65480357-65480376 MS.gene92136:CDS 55.0%
GCCGCACCGCAAAAGGAAAA+GGG - chr8.3:65479746-65479765 MS.gene92136:CDS 55.0%
GCTTCGCGTGCACGTTGTTT+GGG + chr8.3:65479928-65479947 None:intergenic 55.0%
GTTTCTCGCGGGAACCAGTT+GGG + chr8.3:65479843-65479862 None:intergenic 55.0%
TGTTTCTCGCGGGAACCAGT+TGG + chr8.3:65479844-65479863 None:intergenic 55.0%
TTTCTCGCGGGAACCAGTTG+GGG + chr8.3:65479842-65479861 None:intergenic 55.0%
! CGGCCGAGACGAATCTTTTG+AGG - chr8.3:65480196-65480215 MS.gene92136:CDS 55.0%
!! AGCGTTGGAGGAGTTGGTGT+GGG + chr8.3:65479717-65479736 None:intergenic 55.0%
!! GAAGTGGTGTTGGTGCGTTG+AGG + chr8.3:65479894-65479913 None:intergenic 55.0%
!! GAGGATGACAGTGGTGATGG+TGG + chr8.3:65479694-65479713 None:intergenic 55.0%
GCAGCTTCCTTCAAACCCCC+GGG + chr8.3:65480249-65480268 None:intergenic 60.0%
GGGGAGGATGACAGTGGTGA+TGG + chr8.3:65479697-65479716 None:intergenic 60.0%
TGGTGTGGGGAGGATGACAG+TGG + chr8.3:65479703-65479722 None:intergenic 60.0%
! TCCTTTTGCGGTGCGGCTGA+CGG + chr8.3:65479743-65479762 None:intergenic 60.0%
!! CAGCGTTGGAGGAGTTGGTG+TGG + chr8.3:65479718-65479737 None:intergenic 60.0%
!! GCGTTGGAGGAGTTGGTGTG+GGG + chr8.3:65479716-65479735 None:intergenic 60.0%
!! TTGGAGGAGTTGGTGTGGGG+AGG + chr8.3:65479713-65479732 None:intergenic 60.0%
ACCGGATCCGAACCCACACC+CGG - chr8.3:65479814-65479833 MS.gene92136:CDS 65.0%
ACCGGGTGTGGGTTCGGATC+CGG + chr8.3:65479818-65479837 None:intergenic 65.0%
TCCGTCAGCCGCACCGCAAA+AGG - chr8.3:65479739-65479758 MS.gene92136:CDS 65.0%
! CGGAGCAGCGTTGGAGGAGT+TGG + chr8.3:65479723-65479742 None:intergenic 65.0%
GCGGGAACCAGTTGGGGCAC+CGG + chr8.3:65479836-65479855 None:intergenic 70.0%
TGCGGCTGACGGAGCAGCGT+TGG + chr8.3:65479732-65479751 None:intergenic 70.0%
! CGGGAACCAGTTGGGGCACC+GGG + chr8.3:65479835-65479854 None:intergenic 70.0%
! GGCTGACGGAGCAGCGTTGG+AGG + chr8.3:65479729-65479748 None:intergenic 70.0%
!! ACCAGTTGGGGCACCGGGTG+TGG + chr8.3:65479830-65479849 None:intergenic 70.0%
!! CCAGTTGGGGCACCGGGTGT+GGG + chr8.3:65479829-65479848 None:intergenic 70.0%
!! TGGGGCACCGGGTGTGGGTT+CGG + chr8.3:65479824-65479843 None:intergenic 70.0%
CCCACACCCGGTGCCCCAAC+TGG - chr8.3:65479826-65479845 MS.gene92136:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.3 gene 65479667 65480398 65479667 ID=MS.gene92136
chr8.3 mRNA 65479667 65480398 65479667 ID=MS.gene92136.t1;Parent=MS.gene92136
chr8.3 exon 65479667 65480398 65479667 ID=MS.gene92136.t1.exon1;Parent=MS.gene92136.t1
chr8.3 CDS 65479667 65480398 65479667 ID=cds.MS.gene92136.t1;Parent=MS.gene92136.t1
Gene Sequence

>MS.gene92136

ATGCTTCGCCTCACAAAGCTAAAACCACCATCACCACTGTCATCCTCCCCACACCAACTCCTCCAACGCTGCTCCGTCAGCCGCACCGCAAAAGGAAAAGGGAAAGCTGGTCAAATCCTAAAACGTTCCAAAATCACTGTCAAAAAAACCGGATCCGAACCCACACCCGGTGCCCCAACTGGTTCCCGCGAGAAACAAGAACGCGAACGCCTCTACGATCAATGCCTCAACGCACCAACACCACTTCGTTTTCTCAAACCCAAACAACGTGCACGCGAAGCTGAACGAGAAAAATTAGGGTTAATCAGCAAAGATCGTCAAATTGAACTCGATATGATGAAGAAGAAAAACGCGAAGTTTAGGGTTTCTGAGAAGCCAACGATAATGGGAACACCTGGTTTGGATTATATAACGTTAGGGTTAGTGGATGCTGAGAAATTGCCGAAATATGAATTGACGAAAGAAGATGGGATGAAATTGGCGAAGGAGTATAGTAGGGTTTTGATGAGGAAGCATAGGGCGAGACAAGCGGCCGAGACGAATCTTTTGAGGATGAAAAAGGAGGCTATTGAAGCTTTACCCGGGGGTTTGAAGGAAGCTGCTTTGGTTCCGGATTTGACTCCGTTTCCGGTGAATAGGTTTATGGCGACGCTTACGCCACCTATTGATGGGTATATTGAGAAGATTAAGGAGGCTGCTGATAGGATCAGTGGGAAGGAGAAGATTAGGTAG

Protein sequence

>MS.gene92136.t1

MLRLTKLKPPSPLSSSPHQLLQRCSVSRTAKGKGKAGQILKRSKITVKKTGSEPTPGAPTGSREKQERERLYDQCLNAPTPLRFLKPKQRAREAEREKLGLISKDRQIELDMMKKKNAKFRVSEKPTIMGTPGLDYITLGLVDAEKLPKYELTKEDGMKLAKEYSRVLMRKHRARQAAETNLLRMKKEAIEALPGGLKEAALVPDLTPFPVNRFMATLTPPIDGYIEKIKEAADRISGKEKIR