AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene81077


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81077.t1 MTR_8g023960 96.667 240 8 0 1 240 57 296 1.02e-169 469
MS.gene81077.t1 MTR_8g023960 96.667 240 8 0 1 240 1 240 2.53e-169 466
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene81077.t1 AT4G05400 55.731 253 96 5 1 240 1 250 3.06e-85 254
MS.gene81077.t1 AT4G05400 55.731 253 96 5 1 240 1 250 3.06e-85 254
MS.gene81077.t1 AT4G05400 55.731 253 96 5 1 240 1 250 3.06e-85 254
MS.gene81077.t1 AT4G21140 54.286 245 105 5 1 240 1 243 6.38e-73 222

Find 73 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTTCCGGTGAATATGTTTA+TGG 0.209095 8.4:+71058203 MS.gene81077:CDS
AGCTTCCCGTGCGCGTTGTT+TGG 0.253091 8.4:-71057839 None:intergenic
TCCGGTTGGGGCTCCAGGTG+TGG 0.303320 8.4:-71057740 None:intergenic
GTTTCTCGCGGGATCCGGTT+GGG 0.334744 8.4:-71057753 None:intergenic
CGGGATCCGGTTGGGGCTCC+AGG 0.338808 8.4:-71057745 None:intergenic
GCTTCCCGTGCGCGTTGTTT+GGG 0.343871 8.4:-71057838 None:intergenic
GGGTTGGATTATATCACGTT+AGG 0.360005 8.4:+71057976 MS.gene81077:CDS
GGGTTTGAAGGAAGCTGCTT+TGG 0.367116 8.4:+71058164 MS.gene81077:CDS
TCAGTGGGAAGGAGAAGATT+AGG 0.382186 8.4:+71058286 MS.gene81077:CDS
TGGGGCTCCAGGTGTGGGTT+CGG 0.385988 8.4:-71057734 None:intergenic
GAAGGAAGATGGGATGAAAT+TGG 0.390189 8.4:+71058038 MS.gene81077:CDS
TTATATCACGTTAGGGTTAG+TGG 0.395935 8.4:+71057984 MS.gene81077:CDS
TCCTTTGGCGGTGCGGCTGA+CGG 0.396279 8.4:-71057653 None:intergenic
TTCTTGTTTCTCGCGGGATC+CGG 0.402017 8.4:-71057758 None:intergenic
GAGGCTATTGAAGCTTTGCC+TGG 0.402944 8.4:+71058141 MS.gene81077:CDS
TTTCTCGCGGGATCCGGTTG+GGG 0.418787 8.4:-71057752 None:intergenic
AATTGGCCAAGGAGTATAGT+AGG 0.424942 8.4:+71058055 MS.gene81077:CDS
ACGCTTACGCCGCCTATTGA+TGG 0.425057 8.4:+71058228 MS.gene81077:CDS
TCCAGGTGTGGGTTCGGACC+CGG 0.427457 8.4:-71057728 None:intergenic
AGCCGCACCGCCAAAGGAAA+AGG 0.428181 8.4:+71057658 MS.gene81077:CDS
CCGGTTGGGGCTCCAGGTGT+GGG 0.437586 8.4:-71057739 None:intergenic
ATAAACATATTCACCGGAAA+CGG 0.439011 8.4:-71058201 None:intergenic
CCCACACCTGGAGCCCCAAC+CGG 0.442696 8.4:+71057739 MS.gene81077:CDS
TGGGTATATTGAGAAGATTA+AGG 0.447659 8.4:+71058248 MS.gene81077:CDS
CGGAGCAGCGTTGGAGGAGT+TGG 0.459479 8.4:-71057633 None:intergenic
ACCGGGTCCGAACCCACACC+TGG 0.460493 8.4:+71057727 MS.gene81077:CDS
TGCGGCTGACGGAGCAGCGT+TGG 0.460867 8.4:-71057642 None:intergenic
TATGAATTGACGAAGGAAGA+TGG 0.461364 8.4:+71058027 MS.gene81077:CDS
AGATTAAGGAGGCTGCTGAT+AGG 0.463125 8.4:+71058262 MS.gene81077:CDS
ACCGGAAACGGCGTAAAATC+CGG 0.463153 8.4:-71058189 None:intergenic
TCCTTCGTCAATTCATACTT+AGG 0.464649 8.4:-71058021 None:intergenic
TTCTCAATATACCCATCAAT+AGG 0.469115 8.4:-71058240 None:intergenic
TGTTTCTCGCGGGATCCGGT+TGG 0.471217 8.4:-71057754 None:intergenic
GAAGGAAGCTGCTTTGGTGC+CGG 0.473891 8.4:+71058170 MS.gene81077:CDS
GGTTGGATTATATCACGTTA+GGG 0.480423 8.4:+71057977 MS.gene81077:CDS
CCAACGATAATGGGAACCCC+TGG 0.483698 8.4:+71057955 MS.gene81077:CDS
GGCTGACGGAGCAGCGTTGG+AGG 0.488541 8.4:-71057639 None:intergenic
ATGAATTGACGAAGGAAGAT+GGG 0.491364 8.4:+71058028 MS.gene81077:CDS
GGCTATTGAAGCTTTGCCTG+GGG 0.503885 8.4:+71058143 MS.gene81077:CDS
TTGAGAAAACGAAGCGGTGT+TGG 0.505889 8.4:-71057814 None:intergenic
CCAGGGGTTCCCATTATCGT+TGG 0.516292 8.4:-71057955 None:intergenic
CGCTTACGCCGCCTATTGAT+GGG 0.516719 8.4:+71058229 MS.gene81077:CDS
TCCGTCAGCCGCACCGCCAA+AGG 0.523259 8.4:+71057652 MS.gene81077:CDS
GCCAAAGGAAAAGGAAAAGC+CGG 0.524257 8.4:+71057667 MS.gene81077:CDS
AGGCTATTGAAGCTTTGCCT+GGG 0.539955 8.4:+71058142 MS.gene81077:CDS
TTCGCGTTCTTGTTTCTCGC+GGG 0.542290 8.4:-71057764 None:intergenic
TAGCTTTGTGAGACGGAGCA+TGG 0.544288 8.4:-71057587 None:intergenic
CAAAACCCTACTATACTCCT+TGG 0.553845 8.4:-71058061 None:intergenic
GATAATGGGAACCCCTGGGT+TGG 0.575407 8.4:+71057960 MS.gene81077:CDS
ATTGGCCAAGGAGTATAGTA+GGG 0.576553 8.4:+71058056 MS.gene81077:CDS
TCAAACCCAAACAACGCGCA+CGG 0.580702 8.4:+71057833 MS.gene81077:CDS
GTTCGCGTTCTTGTTTCTCG+CGG 0.581626 8.4:-71057765 None:intergenic
CAAACCCAAACAACGCGCAC+GGG 0.595471 8.4:+71057834 MS.gene81077:CDS
GAAGCATAGGGCGAGACAAG+CGG 0.597439 8.4:+71058089 MS.gene81077:CDS
GCTATTGAAGCTTTGCCTGG+GGG 0.597757 8.4:+71058144 MS.gene81077:CDS
TGCTGATAGGATCAGTGGGA+AGG 0.607298 8.4:+71058275 MS.gene81077:CDS
GAGGCTGCTGATAGGATCAG+TGG 0.610487 8.4:+71058270 MS.gene81077:CDS
AGATGGGATGAAATTGGCCA+AGG 0.616560 8.4:+71058044 MS.gene81077:CDS
AACGTGATATAATCCAACCC+AGG 0.616818 8.4:-71057973 None:intergenic
ACGTGATATAATCCAACCCA+GGG 0.616998 8.4:-71057972 None:intergenic
CAACGATAATGGGAACCCCT+GGG 0.617131 8.4:+71057956 MS.gene81077:CDS
AGGCTGCTGATAGGATCAGT+GGG 0.618985 8.4:+71058271 MS.gene81077:CDS
GAAGCGGTGTTGGTGCATTG+AGG 0.621239 8.4:-71057804 None:intergenic
GTTGCCATAAACATATTCAC+CGG 0.628444 8.4:-71058207 None:intergenic
CAGCGTTGGAGGAGTTGGTG+CGG 0.641635 8.4:-71057628 None:intergenic
AGTTGGTGCGGTGAAGATGA+CGG 0.654761 8.4:-71057616 None:intergenic
GCATAGGGCGAGACAAGCGG+CGG 0.658080 8.4:+71058092 MS.gene81077:CDS
GCCTAAGTATGAATTGACGA+AGG 0.676673 8.4:+71058020 MS.gene81077:CDS
TCAATATACCCATCAATAGG+CGG 0.683015 8.4:-71058237 None:intergenic
GTATATTGAGAAGATTAAGG+AGG 0.685736 8.4:+71058251 MS.gene81077:CDS
TGGTGCGGTGAAGATGACGG+TGG 0.737129 8.4:-71057613 None:intergenic
TTGGGTTTGAGAAAACGAAG+CGG 0.742669 8.4:-71057820 None:intergenic
CGTGATATAATCCAACCCAG+GGG 0.794978 8.4:-71057971 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATCACAGTAAAAAAAAC+CGG + chr8.4:71057709-71057728 MS.gene81077:CDS 20.0%
! AAATCACAGTAAAAAAAACC+GGG + chr8.4:71057710-71057729 MS.gene81077:CDS 25.0%
!!! GGTTTTTTTTACTGTGATTT+TGG - chr8.4:71057710-71057729 None:intergenic 25.0%
!!! TTTTTCAGAAACCCTAAATT+TGG - chr8.4:71057935-71057954 None:intergenic 25.0%
AAGCTGAACGAGAAAAATTA+GGG + chr8.4:71057857-71057876 MS.gene81077:CDS 30.0%
AGAAGAAAAACGCCAAATTT+AGG + chr8.4:71057920-71057939 MS.gene81077:CDS 30.0%
ATAAACATATTCACCGGAAA+CGG - chr8.4:71058204-71058223 None:intergenic 30.0%
GAAGAAAAACGCCAAATTTA+GGG + chr8.4:71057921-71057940 MS.gene81077:CDS 30.0%
GTATATTGAGAAGATTAAGG+AGG + chr8.4:71058251-71058270 MS.gene81077:CDS 30.0%
TCTGAAAAACCAACGATAAT+GGG + chr8.4:71057946-71057965 MS.gene81077:CDS 30.0%
TGGGTATATTGAGAAGATTA+AGG + chr8.4:71058248-71058267 MS.gene81077:CDS 30.0%
TTCTCAATATACCCATCAAT+AGG - chr8.4:71058243-71058262 None:intergenic 30.0%
! TTCTGAAAAACCAACGATAA+TGG + chr8.4:71057945-71057964 MS.gene81077:CDS 30.0%
!! GAATCTTTTGAGGATGAAAA+AGG + chr8.4:71058119-71058138 MS.gene81077:CDS 30.0%
ATGAATTGACGAAGGAAGAT+GGG + chr8.4:71058028-71058047 MS.gene81077:CDS 35.0%
GAAGCTGAACGAGAAAAATT+AGG + chr8.4:71057856-71057875 MS.gene81077:CDS 35.0%
GGTTGGATTATATCACGTTA+GGG + chr8.4:71057977-71057996 MS.gene81077:CDS 35.0%
GTTGCCATAAACATATTCAC+CGG - chr8.4:71058210-71058229 None:intergenic 35.0%
GTTTCCGGTGAATATGTTTA+TGG + chr8.4:71058203-71058222 MS.gene81077:CDS 35.0%
TATGAATTGACGAAGGAAGA+TGG + chr8.4:71058027-71058046 MS.gene81077:CDS 35.0%
TCAATATACCCATCAATAGG+CGG - chr8.4:71058240-71058259 None:intergenic 35.0%
TCCTTCGTCAATTCATACTT+AGG - chr8.4:71058024-71058043 None:intergenic 35.0%
TTATATCACGTTAGGGTTAG+TGG + chr8.4:71057984-71058003 MS.gene81077:CDS 35.0%
!! AGTATAGTAGGGTTTTGATG+AGG + chr8.4:71058067-71058086 MS.gene81077:CDS 35.0%
!! TCTTTTGAGGATGAAAAAGG+AGG + chr8.4:71058122-71058141 MS.gene81077:CDS 35.0%
!!! CTGTGATTTTGGAACGTTTT+AGG - chr8.4:71057699-71057718 None:intergenic 35.0%
AACGTGATATAATCCAACCC+AGG - chr8.4:71057976-71057995 None:intergenic 40.0%
ACGTGATATAATCCAACCCA+GGG - chr8.4:71057975-71057994 None:intergenic 40.0%
CAAAACCCTACTATACTCCT+TGG - chr8.4:71058064-71058083 None:intergenic 40.0%
GAAGGAAGATGGGATGAAAT+TGG + chr8.4:71058038-71058057 MS.gene81077:CDS 40.0%
GCCTAAGTATGAATTGACGA+AGG + chr8.4:71058020-71058039 MS.gene81077:CDS 40.0%
GGGTTGGATTATATCACGTT+AGG + chr8.4:71057976-71057995 MS.gene81077:CDS 40.0%
TTGGGTTTGAGAAAACGAAG+CGG - chr8.4:71057823-71057842 None:intergenic 40.0%
! AATTGGCCAAGGAGTATAGT+AGG + chr8.4:71058055-71058074 MS.gene81077:CDS 40.0%
! ATTGGCCAAGGAGTATAGTA+GGG + chr8.4:71058056-71058075 MS.gene81077:CDS 40.0%
!! GGTTTTGATGAGGAAGCATA+GGG + chr8.4:71058077-71058096 MS.gene81077:CDS 40.0%
!!! GGAACGTTTTAGGATTTGAC+CGG - chr8.4:71057689-71057708 None:intergenic 40.0%
!!! GTGGTTTTAGCTTTGTGAGA+CGG - chr8.4:71057597-71057616 None:intergenic 40.0%
AGATGGGATGAAATTGGCCA+AGG + chr8.4:71058044-71058063 MS.gene81077:CDS 45.0%
AGATTAAGGAGGCTGCTGAT+AGG + chr8.4:71058262-71058281 MS.gene81077:CDS 45.0%
AGGCTATTGAAGCTTTGCCT+GGG + chr8.4:71058142-71058161 MS.gene81077:CDS 45.0%
CGTGATATAATCCAACCCAG+GGG - chr8.4:71057974-71057993 None:intergenic 45.0%
GCCAAAGGAAAAGGAAAAGC+CGG + chr8.4:71057667-71057686 MS.gene81077:CDS 45.0%
TCAGTGGGAAGGAGAAGATT+AGG + chr8.4:71058286-71058305 MS.gene81077:CDS 45.0%
TTGAGAAAACGAAGCGGTGT+TGG - chr8.4:71057817-71057836 None:intergenic 45.0%
! GGCTTTTCCTTTTCCTTTGG+CGG - chr8.4:71057668-71057687 None:intergenic 45.0%
!! ACCGGCTTTTCCTTTTCCTT+TGG - chr8.4:71057671-71057690 None:intergenic 45.0%
!! GGGTTTTGATGAGGAAGCAT+AGG + chr8.4:71058076-71058095 MS.gene81077:CDS 45.0%
ACCGGAAACGGCGTAAAATC+CGG - chr8.4:71058192-71058211 None:intergenic 50.0%
AGGCTGCTGATAGGATCAGT+GGG + chr8.4:71058271-71058290 MS.gene81077:CDS 50.0%
GAGGCTATTGAAGCTTTGCC+TGG + chr8.4:71058141-71058160 MS.gene81077:CDS 50.0%
GCTATTGAAGCTTTGCCTGG+GGG + chr8.4:71058144-71058163 MS.gene81077:CDS 50.0%
GGCTATTGAAGCTTTGCCTG+GGG + chr8.4:71058143-71058162 MS.gene81077:CDS 50.0%
GGGTTTGAAGGAAGCTGCTT+TGG + chr8.4:71058164-71058183 MS.gene81077:CDS 50.0%
TCAAACCCAAACAACGCGCA+CGG + chr8.4:71057833-71057852 MS.gene81077:CDS 50.0%
TGCTGATAGGATCAGTGGGA+AGG + chr8.4:71058275-71058294 MS.gene81077:CDS 50.0%
! AGTTGGTGCGGTGAAGATGA+CGG - chr8.4:71057619-71057638 None:intergenic 50.0%
! GTTCGCGTTCTTGTTTCTCG+CGG - chr8.4:71057768-71057787 None:intergenic 50.0%
! TTCCTTTTCCTTTGGCGGTG+CGG - chr8.4:71057663-71057682 None:intergenic 50.0%
! TTCGCGTTCTTGTTTCTCGC+GGG - chr8.4:71057767-71057786 None:intergenic 50.0%
! TTCTTGTTTCTCGCGGGATC+CGG - chr8.4:71057761-71057780 None:intergenic 50.0%
!! CAACGATAATGGGAACCCCT+GGG + chr8.4:71057956-71057975 MS.gene81077:CDS 50.0%
ACGCTTACGCCGCCTATTGA+TGG + chr8.4:71058228-71058247 MS.gene81077:CDS 55.0%
AGCTTTGCCTGGGGGTTTGA+AGG + chr8.4:71058152-71058171 MS.gene81077:CDS 55.0%
CAAACCCAAACAACGCGCAC+GGG + chr8.4:71057834-71057853 MS.gene81077:CDS 55.0%
CCAGGGGTTCCCATTATCGT+TGG - chr8.4:71057958-71057977 None:intergenic 55.0%
CGCTTACGCCGCCTATTGAT+GGG + chr8.4:71058229-71058248 MS.gene81077:CDS 55.0%
GAAGCATAGGGCGAGACAAG+CGG + chr8.4:71058089-71058108 MS.gene81077:CDS 55.0%
GAGGCTGCTGATAGGATCAG+TGG + chr8.4:71058270-71058289 MS.gene81077:CDS 55.0%
! CGGCGGAGACGAATCTTTTG+AGG + chr8.4:71058109-71058128 MS.gene81077:CDS 55.0%
! GAAGGAAGCTGCTTTGGTGC+CGG + chr8.4:71058170-71058189 MS.gene81077:CDS 55.0%
! GCCGGATTTTACGCCGTTTC+CGG + chr8.4:71058188-71058207 MS.gene81077:CDS 55.0%
!! CCAACGATAATGGGAACCCC+TGG + chr8.4:71057955-71057974 MS.gene81077:CDS 55.0%
!! GAAGCGGTGTTGGTGCATTG+AGG - chr8.4:71057807-71057826 None:intergenic 55.0%
!! GATAATGGGAACCCCTGGGT+TGG + chr8.4:71057960-71057979 MS.gene81077:CDS 55.0%
AGCCGCACCGCCAAAGGAAA+AGG + chr8.4:71057658-71057677 MS.gene81077:CDS 60.0%
AGCTTCCCGTGCGCGTTGTT+TGG - chr8.4:71057842-71057861 None:intergenic 60.0%
GCAGCTTCCTTCAAACCCCC+AGG - chr8.4:71058162-71058181 None:intergenic 60.0%
GCTTCCCGTGCGCGTTGTTT+GGG - chr8.4:71057841-71057860 None:intergenic 60.0%
GTTTCTCGCGGGATCCGGTT+GGG - chr8.4:71057756-71057775 None:intergenic 60.0%
TGTTTCTCGCGGGATCCGGT+TGG - chr8.4:71057757-71057776 None:intergenic 60.0%
TTTCTCGCGGGATCCGGTTG+GGG - chr8.4:71057755-71057774 None:intergenic 60.0%
! TGGTGCGGTGAAGATGACGG+TGG - chr8.4:71057616-71057635 None:intergenic 60.0%
!! CAGCGTTGGAGGAGTTGGTG+CGG - chr8.4:71057631-71057650 None:intergenic 60.0%
GCATAGGGCGAGACAAGCGG+CGG + chr8.4:71058092-71058111 MS.gene81077:CDS 65.0%
TCCAGGTGTGGGTTCGGACC+CGG - chr8.4:71057731-71057750 None:intergenic 65.0%
TCCTTTGGCGGTGCGGCTGA+CGG - chr8.4:71057656-71057675 None:intergenic 65.0%
TGGGGCTCCAGGTGTGGGTT+CGG - chr8.4:71057737-71057756 None:intergenic 65.0%
! CGGAGCAGCGTTGGAGGAGT+TGG - chr8.4:71057636-71057655 None:intergenic 65.0%
ACCGGGTCCGAACCCACACC+TGG + chr8.4:71057727-71057746 MS.gene81077:CDS 70.0%
CCCACACCTGGAGCCCCAAC+CGG + chr8.4:71057739-71057758 MS.gene81077:CDS 70.0%
CCGGTTGGGGCTCCAGGTGT+GGG - chr8.4:71057742-71057761 None:intergenic 70.0%
TCCGGTTGGGGCTCCAGGTG+TGG - chr8.4:71057743-71057762 None:intergenic 70.0%
TCCGTCAGCCGCACCGCCAA+AGG + chr8.4:71057652-71057671 MS.gene81077:CDS 70.0%
TGCGGCTGACGGAGCAGCGT+TGG - chr8.4:71057645-71057664 None:intergenic 70.0%
! GGCTGACGGAGCAGCGTTGG+AGG - chr8.4:71057642-71057661 None:intergenic 70.0%
CGGGATCCGGTTGGGGCTCC+AGG - chr8.4:71057748-71057767 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.4 gene 71057589 71058311 71057589 ID=MS.gene81077
chr8.4 mRNA 71057589 71058311 71057589 ID=MS.gene81077.t1;Parent=MS.gene81077
chr8.4 exon 71057589 71058311 71057589 ID=MS.gene81077.t1.exon1;Parent=MS.gene81077.t1
chr8.4 CDS 71057589 71058311 71057589 ID=cds.MS.gene81077.t1;Parent=MS.gene81077.t1
Gene Sequence

>MS.gene81077

ATGCTCCGTCTCACAAAGCTAAAACCACCGTCATCTTCACCGCACCAACTCCTCCAACGCTGCTCCGTCAGCCGCACCGCCAAAGGAAAAGGAAAAGCCGGTCAAATCCTAAAACGTTCCAAAATCACAGTAAAAAAAACCGGGTCCGAACCCACACCTGGAGCCCCAACCGGATCCCGCGAGAAACAAGAACGCGAACGTCTCTACGATCAATGCCTCAATGCACCAACACCGCTTCGTTTTCTCAAACCCAAACAACGCGCACGGGAAGCTGAACGAGAAAAATTAGGGTTAATTAGCAAAGATCGTCAAATTGAACTTGATATGATGAAGAAGAAAAACGCCAAATTTAGGGTTTCTGAAAAACCAACGATAATGGGAACCCCTGGGTTGGATTATATCACGTTAGGGTTAGTGGATGCTGAGAAATTGCCTAAGTATGAATTGACGAAGGAAGATGGGATGAAATTGGCCAAGGAGTATAGTAGGGTTTTGATGAGGAAGCATAGGGCGAGACAAGCGGCGGAGACGAATCTTTTGAGGATGAAAAAGGAGGCTATTGAAGCTTTGCCTGGGGGTTTGAAGGAAGCTGCTTTGGTGCCGGATTTTACGCCGTTTCCGGTGAATATGTTTATGGCAACGCTTACGCCGCCTATTGATGGGTATATTGAGAAGATTAAGGAGGCTGCTGATAGGATCAGTGGGAAGGAGAAGATTAGGTAG

Protein sequence

>MS.gene81077.t1

MLRLTKLKPPSSSPHQLLQRCSVSRTAKGKGKAGQILKRSKITVKKTGSEPTPGAPTGSREKQERERLYDQCLNAPTPLRFLKPKQRAREAEREKLGLISKDRQIELDMMKKKNAKFRVSEKPTIMGTPGLDYITLGLVDAEKLPKYELTKEDGMKLAKEYSRVLMRKHRARQAAETNLLRMKKEAIEALPGGLKEAALVPDFTPFPVNMFMATLTPPIDGYIEKIKEAADRISGKEKIR