AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene59614


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59614.t1 MTR_8g023960 94.650 243 10 1 1 243 57 296 1.36e-165 459
MS.gene59614.t1 MTR_8g023960 94.650 243 10 1 1 243 1 240 4.31e-165 456
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene59614.t1 AT4G05400 56.078 255 95 5 1 243 1 250 2.33e-88 262
MS.gene59614.t1 AT4G05400 56.078 255 95 5 1 243 1 250 2.33e-88 262
MS.gene59614.t1 AT4G05400 56.078 255 95 5 1 243 1 250 2.33e-88 262
MS.gene59614.t1 AT4G21140 55.061 247 103 5 1 243 1 243 1.16e-74 227

Find 72 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCGGATTTGACTCCGTTTC+CGG 0.238916 8.1:-73605784 MS.gene59614:CDS
GAAGCTGAACGAGAGAAATT+AGG 0.242522 8.1:-73606116 MS.gene59614:CDS
GTTTCCGGTGAATAGGTTTA+TGG 0.264318 8.1:-73605769 MS.gene59614:CDS
ACCAGTTGGGGCTCCGGATG+TGG 0.279909 8.1:+73606232 None:intergenic
TCTTTCGTCAATTCATATTT+CGG 0.289163 8.1:+73605951 None:intergenic
AGCTTCGCGTGCACGTTGTT+TGG 0.290006 8.1:+73606133 None:intergenic
TTCTGAGAAACCAACGATAA+TGG 0.292493 8.1:-73606027 MS.gene59614:CDS
GTTTCTCGCGGGAACCAGTT+GGG 0.347840 8.1:+73606219 None:intergenic
GCTTCGCGTGCACGTTGTTT+GGG 0.366189 8.1:+73606134 None:intergenic
TGGGGCTCCGGATGTGGGTT+CGG 0.366533 8.1:+73606238 None:intergenic
GGTTTGGATTATATAACGTT+AGG 0.370627 8.1:-73605996 MS.gene59614:CDS
GATAATGGGAACACCTGGTT+TGG 0.375812 8.1:-73606012 MS.gene59614:CDS
TCAGTGGGAAGGAGAAGATT+AGG 0.382186 8.1:-73605686 MS.gene59614:CDS
GAAAGAAGATGGGATGAAAT+TGG 0.382332 8.1:-73605934 MS.gene59614:CDS
AGGTTTGAAGGAAGCTGCTT+TGG 0.388226 8.1:-73605808 MS.gene59614:CDS
TTATATAACGTTAGGGTTAG+TGG 0.388954 8.1:-73605988 MS.gene59614:CDS
TCCTTTGGCGGTGCGGCTGA+CGG 0.396279 8.1:+73606319 None:intergenic
TGACTCCGTTTCCGGTGAAT+AGG 0.396696 8.1:-73605776 MS.gene59614:CDS
TCCGGATGTGGGTTCGGATC+CGG 0.397530 8.1:+73606244 None:intergenic
AATTGGCGAAGGAGTATAGT+AGG 0.407802 8.1:-73605917 MS.gene59614:CDS
TCTGAGAAACCAACGATAAT+GGG 0.408601 8.1:-73606026 MS.gene59614:CDS
AAGCTGAACGAGAGAAATTA+GGG 0.425043 8.1:-73606115 MS.gene59614:CDS
AGCCGCACCGCCAAAGGAAA+AGG 0.428181 8.1:-73606314 MS.gene59614:CDS
CCCACATCCGGAGCCCCAAC+TGG 0.437112 8.1:-73606233 MS.gene59614:CDS
TATGAATTGACGAAAGAAGA+TGG 0.442226 8.1:-73605945 MS.gene59614:CDS
TGGGTATATTGAGAAGATTA+AGG 0.447659 8.1:-73605724 MS.gene59614:CDS
AGCTTTACCTGGAGGTTTGA+AGG 0.450403 8.1:-73605820 MS.gene59614:CDS
TGTTTCTCGCGGGAACCAGT+TGG 0.454283 8.1:+73606218 None:intergenic
CCAGTTGGGGCTCCGGATGT+GGG 0.456017 8.1:+73606233 None:intergenic
ATAAACCTATTCACCGGAAA+CGG 0.456384 8.1:+73605771 None:intergenic
CGGAGCAGCGTTGGAGGAGT+TGG 0.459479 8.1:+73606339 None:intergenic
TGCGGCTGACGGAGCAGCGT+TGG 0.460867 8.1:+73606330 None:intergenic
GCGGGAACCAGTTGGGGCTC+CGG 0.461594 8.1:+73606226 None:intergenic
AGATTAAGGAGGCTGCTGAT+AGG 0.463125 8.1:-73605710 MS.gene59614:CDS
GTTTGGATTATATAACGTTA+GGG 0.470062 8.1:-73605995 MS.gene59614:CDS
TTCTCAATATACCCATCAAT+AGG 0.473537 8.1:+73605732 None:intergenic
GAAGGAAGCTGCTTTGGTGC+CGG 0.473891 8.1:-73605802 MS.gene59614:CDS
GAGGCAATTGAAGCTTTACC+TGG 0.482431 8.1:-73605831 MS.gene59614:CDS
GGCTGACGGAGCAGCGTTGG+AGG 0.488541 8.1:+73606333 None:intergenic
ACCGGAAACGGAGTCAAATC+CGG 0.495763 8.1:+73605783 None:intergenic
TTTCTCGCGGGAACCAGTTG+GGG 0.496436 8.1:+73606220 None:intergenic
ACGCTTACGCCACCTATTGA+TGG 0.498561 8.1:-73605744 MS.gene59614:CDS
ACCGGATCCGAACCCACATC+CGG 0.501666 8.1:-73606245 MS.gene59614:CDS
TTCGCGTTCTTGTTTCTCGC+GGG 0.513895 8.1:+73606208 None:intergenic
ATGAATTGACGAAAGAAGAT+GGG 0.521993 8.1:-73605944 MS.gene59614:CDS
TCCGTCAGCCGCACCGCCAA+AGG 0.523259 8.1:-73606320 MS.gene59614:CDS
GCCAAAGGAAAAGGAAAAGC+CGG 0.524257 8.1:-73606305 MS.gene59614:CDS
CATCCTCAAAAGATTCGTCT+CGG 0.529672 8.1:+73605860 None:intergenic
CCAGGTGTTCCCATTATCGT+TGG 0.533219 8.1:+73606017 None:intergenic
CGCTTACGCCACCTATTGAT+GGG 0.534577 8.1:-73605743 MS.gene59614:CDS
GCAGCTTCCTTCAAACCTCC+AGG 0.536551 8.1:+73605813 None:intergenic
GTTCGCGTTCTTGTTTCTCG+CGG 0.547502 8.1:+73606207 None:intergenic
ATTGGCGAAGGAGTATAGTA+GGG 0.549849 8.1:-73605916 MS.gene59614:CDS
CCAACGATAATGGGAACACC+TGG 0.558342 8.1:-73606017 MS.gene59614:CDS
TTGAGAAAACGAAGTGGTGT+TGG 0.571375 8.1:+73606158 None:intergenic
AGATGGGATGAAATTGGCGA+AGG 0.597418 8.1:-73605928 MS.gene59614:CDS
GAAGCATAGGGCGAGACAAG+CGG 0.597439 8.1:-73605883 MS.gene59614:CDS
TGCTGATAGGATCAGTGGGA+AGG 0.607298 8.1:-73605697 MS.gene59614:CDS
GGTGAAGATGACAGTGGTGA+TGG 0.610256 8.1:+73606365 None:intergenic
GAGGCTGCTGATAGGATCAG+TGG 0.610487 8.1:-73605702 MS.gene59614:CDS
GTCGCCATAAACCTATTCAC+CGG 0.613259 8.1:+73605765 None:intergenic
AGGCTGCTGATAGGATCAGT+GGG 0.618985 8.1:-73605701 MS.gene59614:CDS
CAGCGTTGGAGGAGTTGGTG+CGG 0.641635 8.1:+73606344 None:intergenic
TCAATATACCCATCAATAGG+TGG 0.649441 8.1:+73605735 None:intergenic
GCAATTGAAGCTTTACCTGG+AGG 0.659327 8.1:-73605828 MS.gene59614:CDS
CGTTGAGGCATTGATCGTAG+AGG 0.672109 8.1:+73606183 None:intergenic
AACGTTATATAATCCAAACC+AGG 0.673172 8.1:+73605999 None:intergenic
GAAGATGACAGTGGTGATGG+TGG 0.683610 8.1:+73606368 None:intergenic
GTATATTGAGAAGATTAAGG+AGG 0.685736 8.1:-73605721 MS.gene59614:CDS
TTGGGTTTGAGAAAACGAAG+TGG 0.743892 8.1:+73606152 None:intergenic
GAAGTGGTGTTGGTGCGTTG+AGG 0.759588 8.1:+73606168 None:intergenic
TGGTGCGGTGAAGATGACAG+TGG 0.785449 8.1:+73606359 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAATCACTGTCAAAAAAAC+CGG - chr8.1:73605812-73605831 MS.gene59614:CDS 25.0%
! GTTTGGATTATATAACGTTA+GGG - chr8.1:73606080-73606099 MS.gene59614:CDS 25.0%
! TCTTTCGTCAATTCATATTT+CGG + chr8.1:73606127-73606146 None:intergenic 25.0%
AACGTTATATAATCCAAACC+AGG + chr8.1:73606079-73606098 None:intergenic 30.0%
ATGAATTGACGAAAGAAGAT+GGG - chr8.1:73606131-73606150 MS.gene59614:CDS 30.0%
GGTTTGGATTATATAACGTT+AGG - chr8.1:73606079-73606098 MS.gene59614:CDS 30.0%
GTATATTGAGAAGATTAAGG+AGG - chr8.1:73606354-73606373 MS.gene59614:CDS 30.0%
TATGAATTGACGAAAGAAGA+TGG - chr8.1:73606130-73606149 MS.gene59614:CDS 30.0%
TGGGTATATTGAGAAGATTA+AGG - chr8.1:73606351-73606370 MS.gene59614:CDS 30.0%
TTATATAACGTTAGGGTTAG+TGG - chr8.1:73606087-73606106 MS.gene59614:CDS 30.0%
TTCTCAATATACCCATCAAT+AGG + chr8.1:73606346-73606365 None:intergenic 30.0%
!! GAATCTTTTGAGGATGAAAA+AGG - chr8.1:73606222-73606241 MS.gene59614:CDS 30.0%
!!! GGTTTTTTTGACAGTGATTT+TGG + chr8.1:73605813-73605832 None:intergenic 30.0%
AAGCTGAACGAGAGAAATTA+GGG - chr8.1:73605960-73605979 MS.gene59614:CDS 35.0%
AGAAGAAAAACGCGAAGTTT+AGG - chr8.1:73606023-73606042 MS.gene59614:CDS 35.0%
ATAAACCTATTCACCGGAAA+CGG + chr8.1:73606307-73606326 None:intergenic 35.0%
GAAAGAAGATGGGATGAAAT+TGG - chr8.1:73606141-73606160 MS.gene59614:CDS 35.0%
GAAGAAAAACGCGAAGTTTA+GGG - chr8.1:73606024-73606043 MS.gene59614:CDS 35.0%
TCAATATACCCATCAATAGG+TGG + chr8.1:73606343-73606362 None:intergenic 35.0%
TCTGAGAAACCAACGATAAT+GGG - chr8.1:73606049-73606068 MS.gene59614:CDS 35.0%
! TTCTGAGAAACCAACGATAA+TGG - chr8.1:73606048-73606067 MS.gene59614:CDS 35.0%
!! AGTATAGTAGGGTTTTGATG+AGG - chr8.1:73606170-73606189 MS.gene59614:CDS 35.0%
!! TCTTTTGAGGATGAAAAAGG+AGG - chr8.1:73606225-73606244 MS.gene59614:CDS 35.0%
!!! CAGTGATTTTGGAACGTTTT+AGG + chr8.1:73605802-73605821 None:intergenic 35.0%
CATCCTCAAAAGATTCGTCT+CGG + chr8.1:73606218-73606237 None:intergenic 40.0%
GAAGCTGAACGAGAGAAATT+AGG - chr8.1:73605959-73605978 MS.gene59614:CDS 40.0%
GTTTCCGGTGAATAGGTTTA+TGG - chr8.1:73606306-73606325 MS.gene59614:CDS 40.0%
TTGAGAAAACGAAGTGGTGT+TGG + chr8.1:73605920-73605939 None:intergenic 40.0%
TTGGGTTTGAGAAAACGAAG+TGG + chr8.1:73605926-73605945 None:intergenic 40.0%
! AATTGGCGAAGGAGTATAGT+AGG - chr8.1:73606158-73606177 MS.gene59614:CDS 40.0%
! ATTGGCGAAGGAGTATAGTA+GGG - chr8.1:73606159-73606178 MS.gene59614:CDS 40.0%
!! GGTTTTGATGAGGAAGCATA+GGG - chr8.1:73606180-73606199 MS.gene59614:CDS 40.0%
!!! GGAACGTTTTAGGATTTGAC+CGG + chr8.1:73605792-73605811 None:intergenic 40.0%
AGATGGGATGAAATTGGCGA+AGG - chr8.1:73606147-73606166 MS.gene59614:CDS 45.0%
AGATTAAGGAGGCTGCTGAT+AGG - chr8.1:73606365-73606384 MS.gene59614:CDS 45.0%
AGCTTTACCTGGAGGTTTGA+AGG - chr8.1:73606255-73606274 MS.gene59614:CDS 45.0%
AGGTTTGAAGGAAGCTGCTT+TGG - chr8.1:73606267-73606286 MS.gene59614:CDS 45.0%
GAGGCAATTGAAGCTTTACC+TGG - chr8.1:73606244-73606263 MS.gene59614:CDS 45.0%
GCAATTGAAGCTTTACCTGG+AGG - chr8.1:73606247-73606266 MS.gene59614:CDS 45.0%
GCCAAAGGAAAAGGAAAAGC+CGG - chr8.1:73605770-73605789 MS.gene59614:CDS 45.0%
GTCGCCATAAACCTATTCAC+CGG + chr8.1:73606313-73606332 None:intergenic 45.0%
TCAGTGGGAAGGAGAAGATT+AGG - chr8.1:73606389-73606408 MS.gene59614:CDS 45.0%
! GGCTTTTCCTTTTCCTTTGG+CGG + chr8.1:73605771-73605790 None:intergenic 45.0%
!! ACCGGCTTTTCCTTTTCCTT+TGG + chr8.1:73605774-73605793 None:intergenic 45.0%
!! GATAATGGGAACACCTGGTT+TGG - chr8.1:73606063-73606082 MS.gene59614:CDS 45.0%
!! GGGTTTTGATGAGGAAGCAT+AGG - chr8.1:73606179-73606198 MS.gene59614:CDS 45.0%
ACCGGAAACGGAGTCAAATC+CGG + chr8.1:73606295-73606314 None:intergenic 50.0%
ACGCTTACGCCACCTATTGA+TGG - chr8.1:73606331-73606350 MS.gene59614:CDS 50.0%
AGGCTGCTGATAGGATCAGT+GGG - chr8.1:73606374-73606393 MS.gene59614:CDS 50.0%
CCAGGTGTTCCCATTATCGT+TGG + chr8.1:73606061-73606080 None:intergenic 50.0%
CGCTTACGCCACCTATTGAT+GGG - chr8.1:73606332-73606351 MS.gene59614:CDS 50.0%
GGTGAAGATGACAGTGGTGA+TGG + chr8.1:73605713-73605732 None:intergenic 50.0%
TGACTCCGTTTCCGGTGAAT+AGG - chr8.1:73606299-73606318 MS.gene59614:CDS 50.0%
TGCTGATAGGATCAGTGGGA+AGG - chr8.1:73606378-73606397 MS.gene59614:CDS 50.0%
! GTTCGCGTTCTTGTTTCTCG+CGG + chr8.1:73605871-73605890 None:intergenic 50.0%
! TTCCTTTTCCTTTGGCGGTG+CGG + chr8.1:73605766-73605785 None:intergenic 50.0%
! TTCGCGTTCTTGTTTCTCGC+GGG + chr8.1:73605870-73605889 None:intergenic 50.0%
!! CCAACGATAATGGGAACACC+TGG - chr8.1:73606058-73606077 MS.gene59614:CDS 50.0%
!! CGTTGAGGCATTGATCGTAG+AGG + chr8.1:73605895-73605914 None:intergenic 50.0%
!! GAAGATGACAGTGGTGATGG+TGG + chr8.1:73605710-73605729 None:intergenic 50.0%
AGCTTCGCGTGCACGTTGTT+TGG + chr8.1:73605945-73605964 None:intergenic 55.0%
GAAGCATAGGGCGAGACAAG+CGG - chr8.1:73606192-73606211 MS.gene59614:CDS 55.0%
GAGGCTGCTGATAGGATCAG+TGG - chr8.1:73606373-73606392 MS.gene59614:CDS 55.0%
GCAGCTTCCTTCAAACCTCC+AGG + chr8.1:73606265-73606284 None:intergenic 55.0%
GCCGGATTTGACTCCGTTTC+CGG - chr8.1:73606291-73606310 MS.gene59614:CDS 55.0%
GCTTCGCGTGCACGTTGTTT+GGG + chr8.1:73605944-73605963 None:intergenic 55.0%
GTTTCTCGCGGGAACCAGTT+GGG + chr8.1:73605859-73605878 None:intergenic 55.0%
TGGTGCGGTGAAGATGACAG+TGG + chr8.1:73605719-73605738 None:intergenic 55.0%
TGTTTCTCGCGGGAACCAGT+TGG + chr8.1:73605860-73605879 None:intergenic 55.0%
TTTCTCGCGGGAACCAGTTG+GGG + chr8.1:73605858-73605877 None:intergenic 55.0%
! CGGCCGAGACGAATCTTTTG+AGG - chr8.1:73606212-73606231 MS.gene59614:CDS 55.0%
! GAAGGAAGCTGCTTTGGTGC+CGG - chr8.1:73606273-73606292 MS.gene59614:CDS 55.0%
!! GAAGTGGTGTTGGTGCGTTG+AGG + chr8.1:73605910-73605929 None:intergenic 55.0%
ACCGGATCCGAACCCACATC+CGG - chr8.1:73605830-73605849 MS.gene59614:CDS 60.0%
AGCCGCACCGCCAAAGGAAA+AGG - chr8.1:73605761-73605780 MS.gene59614:CDS 60.0%
TCCGGATGTGGGTTCGGATC+CGG + chr8.1:73605834-73605853 None:intergenic 60.0%
!! CAGCGTTGGAGGAGTTGGTG+CGG + chr8.1:73605734-73605753 None:intergenic 60.0%
ACCAGTTGGGGCTCCGGATG+TGG + chr8.1:73605846-73605865 None:intergenic 65.0%
CCAGTTGGGGCTCCGGATGT+GGG + chr8.1:73605845-73605864 None:intergenic 65.0%
TCCTTTGGCGGTGCGGCTGA+CGG + chr8.1:73605759-73605778 None:intergenic 65.0%
TGGGGCTCCGGATGTGGGTT+CGG + chr8.1:73605840-73605859 None:intergenic 65.0%
! CGGAGCAGCGTTGGAGGAGT+TGG + chr8.1:73605739-73605758 None:intergenic 65.0%
CCCACATCCGGAGCCCCAAC+TGG - chr8.1:73605842-73605861 MS.gene59614:CDS 70.0%
GCGGGAACCAGTTGGGGCTC+CGG + chr8.1:73605852-73605871 None:intergenic 70.0%
TCCGTCAGCCGCACCGCCAA+AGG - chr8.1:73605755-73605774 MS.gene59614:CDS 70.0%
TGCGGCTGACGGAGCAGCGT+TGG + chr8.1:73605748-73605767 None:intergenic 70.0%
! GGCTGACGGAGCAGCGTTGG+AGG + chr8.1:73605745-73605764 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 73605683 73606414 73605683 ID=MS.gene59614
chr8.1 mRNA 73605683 73606414 73605683 ID=MS.gene59614.t1;Parent=MS.gene59614
chr8.1 exon 73605683 73606414 73605683 ID=MS.gene59614.t1.exon1;Parent=MS.gene59614.t1
chr8.1 CDS 73605683 73606414 73605683 ID=cds.MS.gene59614.t1;Parent=MS.gene59614.t1
Gene Sequence

>MS.gene59614

ATGCTTCGCATCACAAAGCTAAAACCACCATCACCACTGTCATCTTCACCGCACCAACTCCTCCAACGCTGCTCCGTCAGCCGCACCGCCAAAGGAAAAGGAAAAGCCGGTCAAATCCTAAAACGTTCCAAAATCACTGTCAAAAAAACCGGATCCGAACCCACATCCGGAGCCCCAACTGGTTCCCGCGAGAAACAAGAACGCGAACGCCTCTACGATCAATGCCTCAACGCACCAACACCACTTCGTTTTCTCAAACCCAAACAACGTGCACGCGAAGCTGAACGAGAGAAATTAGGGTTAATCAGCAAAGATCGTCAAATTGAACTCGATATGATGAAGAAGAAAAACGCGAAGTTTAGGGTTTCTGAGAAACCAACGATAATGGGAACACCTGGTTTGGATTATATAACGTTAGGGTTAGTGGATGCTGAGAAATTGCCGAAATATGAATTGACGAAAGAAGATGGGATGAAATTGGCGAAGGAGTATAGTAGGGTTTTGATGAGGAAGCATAGGGCGAGACAAGCGGCCGAGACGAATCTTTTGAGGATGAAAAAGGAGGCAATTGAAGCTTTACCTGGAGGTTTGAAGGAAGCTGCTTTGGTGCCGGATTTGACTCCGTTTCCGGTGAATAGGTTTATGGCGACGCTTACGCCACCTATTGATGGGTATATTGAGAAGATTAAGGAGGCTGCTGATAGGATCAGTGGGAAGGAGAAGATTAGGTAG

Protein sequence

>MS.gene59614.t1

MLRITKLKPPSPLSSSPHQLLQRCSVSRTAKGKGKAGQILKRSKITVKKTGSEPTSGAPTGSREKQERERLYDQCLNAPTPLRFLKPKQRAREAEREKLGLISKDRQIELDMMKKKNAKFRVSEKPTIMGTPGLDYITLGLVDAEKLPKYELTKEDGMKLAKEYSRVLMRKHRARQAAETNLLRMKKEAIEALPGGLKEAALVPDLTPFPVNRFMATLTPPIDGYIEKIKEAADRISGKEKIR