AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene98177


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98177.t1 MTR_8g023960 95.062 243 9 1 1 243 57 296 1.42e-166 462
MS.gene98177.t1 MTR_8g023960 95.062 243 9 1 1 243 1 240 5.54e-166 458
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98177.t1 AT4G05400 56.078 255 95 5 1 243 1 250 3.46e-88 261
MS.gene98177.t1 AT4G05400 56.078 255 95 5 1 243 1 250 3.46e-88 261
MS.gene98177.t1 AT4G05400 56.078 255 95 5 1 243 1 250 3.46e-88 261
MS.gene98177.t1 AT4G21140 54.656 247 104 5 1 243 1 243 1.62e-74 226

Find 73 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCAGTTGGGGCTCCGGGTG+TGG 0.229402 8.2:+68996071 None:intergenic
TCCGGATTTGACTCCGTTTC+CGG 0.238916 8.2:-68995623 MS.gene98177:CDS
GAAGCTGAACGAGAGAAATT+AGG 0.242522 8.2:-68995955 MS.gene98177:CDS
GTTTCCGGTGAATAGGTTTA+TGG 0.264318 8.2:-68995608 MS.gene98177:CDS
TCTTTCGTCAATTCATATTT+CGG 0.289163 8.2:+68995790 None:intergenic
AGCTTCGCGTGCACGTTGTT+TGG 0.290006 8.2:+68995972 None:intergenic
TTCTGAGAAGCCAACGATAA+TGG 0.299403 8.2:-68995866 MS.gene98177:CDS
TGGGGCTCCGGGTGTGGGTT+CGG 0.342298 8.2:+68996077 None:intergenic
GAAGGAAGCTGCTTTGGTTC+CGG 0.344771 8.2:-68995641 MS.gene98177:CDS
GTTTCTCGCGGGAACCAGTT+GGG 0.347840 8.2:+68996058 None:intergenic
GGGTTTGAAGGAAGCTGCTT+TGG 0.352372 8.2:-68995647 MS.gene98177:CDS
GCGGGAACCAGTTGGGGCTC+CGG 0.364469 8.2:+68996065 None:intergenic
GCTTCGCGTGCACGTTGTTT+GGG 0.366189 8.2:+68995973 None:intergenic
GGTTTGGATTATATAACGTT+AGG 0.370627 8.2:-68995835 MS.gene98177:CDS
GATAATGGGAACACCTGGTT+TGG 0.375812 8.2:-68995851 MS.gene98177:CDS
TCAGTGGGAAGGAGAAGATT+AGG 0.382186 8.2:-68995525 MS.gene98177:CDS
GAAAGAAGATGGGATGAAAT+TGG 0.382332 8.2:-68995773 MS.gene98177:CDS
TTATATAACGTTAGGGTTAG+TGG 0.388954 8.2:-68995827 MS.gene98177:CDS
CGGGAACCAGTTGGGGCTCC+GGG 0.390448 8.2:+68996066 None:intergenic
CCAGTTGGGGCTCCGGGTGT+GGG 0.394410 8.2:+68996072 None:intergenic
TCCTTTGGCGGTGCGGCTGA+CGG 0.396279 8.2:+68996158 None:intergenic
TGACTCCGTTTCCGGTGAAT+AGG 0.396696 8.2:-68995615 MS.gene98177:CDS
TCCGGGTGTGGGTTCGGATC+CGG 0.397530 8.2:+68996083 None:intergenic
TCTGAGAAGCCAACGATAAT+GGG 0.401787 8.2:-68995865 MS.gene98177:CDS
GAGGCTATTGAAGCTTTGCC+TGG 0.402944 8.2:-68995670 MS.gene98177:CDS
AATTGGCGAAGGAGTATAGT+AGG 0.407802 8.2:-68995756 MS.gene98177:CDS
AAGCTGAACGAGAGAAATTA+GGG 0.425043 8.2:-68995954 MS.gene98177:CDS
AGCCGCACCGCCAAAGGAAA+AGG 0.428181 8.2:-68996153 MS.gene98177:CDS
CCCACACCCGGAGCCCCAAC+TGG 0.437112 8.2:-68996072 MS.gene98177:CDS
TATGAATTGACGAAAGAAGA+TGG 0.442226 8.2:-68995784 MS.gene98177:CDS
ACCGGAAACGGAGTCAAATC+CGG 0.446628 8.2:+68995622 None:intergenic
TGGGTATATTGAGAAGATTA+AGG 0.447659 8.2:-68995563 MS.gene98177:CDS
TGTTTCTCGCGGGAACCAGT+TGG 0.454283 8.2:+68996057 None:intergenic
ATAAACCTATTCACCGGAAA+CGG 0.456384 8.2:+68995610 None:intergenic
TGCGGCTGACGGAGCAGCGT+TGG 0.460867 8.2:+68996169 None:intergenic
AGATTAAGGAGGCTGCTGAT+AGG 0.463125 8.2:-68995549 MS.gene98177:CDS
GTTTGGATTATATAACGTTA+GGG 0.470062 8.2:-68995834 MS.gene98177:CDS
TTCTCAATATACCCATCAAT+AGG 0.473537 8.2:+68995571 None:intergenic
CGGAGCAGCGTTGGAGGAGT+TGG 0.481246 8.2:+68996178 None:intergenic
ACCGGATCCGAACCCACACC+CGG 0.484975 8.2:-68996084 MS.gene98177:CDS
GGCTGACGGAGCAGCGTTGG+AGG 0.488541 8.2:+68996172 None:intergenic
TTTCTCGCGGGAACCAGTTG+GGG 0.496436 8.2:+68996059 None:intergenic
ACGCTTACGCCACCTATTGA+TGG 0.498561 8.2:-68995583 MS.gene98177:CDS
GGCTATTGAAGCTTTGCCTG+GGG 0.503885 8.2:-68995668 MS.gene98177:CDS
TTCACGTTCTTGTTTCTCGC+GGG 0.516113 8.2:+68996047 None:intergenic
ATGAATTGACGAAAGAAGAT+GGG 0.521993 8.2:-68995783 MS.gene98177:CDS
TCCGTCAGCCGCACCGCCAA+AGG 0.523259 8.2:-68996159 MS.gene98177:CDS
GCCAAAGGAAAAGGAAAAGC+CGG 0.524257 8.2:-68996144 MS.gene98177:CDS
CCAGGTGTTCCCATTATCGT+TGG 0.527921 8.2:+68995856 None:intergenic
CGCTTACGCCACCTATTGAT+GGG 0.534577 8.2:-68995582 MS.gene98177:CDS
AGGCTATTGAAGCTTTGCCT+GGG 0.539955 8.2:-68995669 MS.gene98177:CDS
ATTGGCGAAGGAGTATAGTA+GGG 0.549849 8.2:-68995755 MS.gene98177:CDS
GGTGAAGATGACAGTGGTGA+TGG 0.567426 8.2:+68996204 None:intergenic
TTGAGAAAACGAAGTGGTGT+TGG 0.571375 8.2:+68995997 None:intergenic
CCAACGATAATGGGAACACC+TGG 0.593653 8.2:-68995856 MS.gene98177:CDS
GTTCACGTTCTTGTTTCTCG+CGG 0.595018 8.2:+68996046 None:intergenic
AGATGGGATGAAATTGGCGA+AGG 0.597418 8.2:-68995767 MS.gene98177:CDS
GAAGCATAGGGCGAGACAAG+CGG 0.597439 8.2:-68995722 MS.gene98177:CDS
GCTATTGAAGCTTTGCCTGG+GGG 0.597757 8.2:-68995667 MS.gene98177:CDS
TGCTGATAGGATCAGTGGGA+AGG 0.607298 8.2:-68995536 MS.gene98177:CDS
GAGGCTGCTGATAGGATCAG+TGG 0.610487 8.2:-68995541 MS.gene98177:CDS
GTCGCCATAAACCTATTCAC+CGG 0.613259 8.2:+68995604 None:intergenic
AGGCTGCTGATAGGATCAGT+GGG 0.618985 8.2:-68995540 MS.gene98177:CDS
GAAGATGACAGTGGTGATGG+TGG 0.619266 8.2:+68996207 None:intergenic
TCAATATACCCATCAATAGG+TGG 0.649441 8.2:+68995574 None:intergenic
GCATAGGGCGAGACAAGCGG+CGG 0.658080 8.2:-68995719 MS.gene98177:CDS
CAGCGTTGGAGGAGTTGGTG+AGG 0.660011 8.2:+68996183 None:intergenic
CGTTGAGGCATTGATCGTAG+AGG 0.672109 8.2:+68996022 None:intergenic
AACGTTATATAATCCAAACC+AGG 0.673172 8.2:+68995838 None:intergenic
GTATATTGAGAAGATTAAGG+AGG 0.685736 8.2:-68995560 MS.gene98177:CDS
TGGTGAGGTGAAGATGACAG+TGG 0.722654 8.2:+68996198 None:intergenic
TTGGGTTTGAGAAAACGAAG+TGG 0.743892 8.2:+68995991 None:intergenic
GAAGTGGTGTTGGTGCGTTG+AGG 0.759588 8.2:+68996007 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! AAAATCACTGTCAAAAAAAC+CGG - chr8.2:68995651-68995670 MS.gene98177:CDS 25.0%
! GTTTGGATTATATAACGTTA+GGG - chr8.2:68995919-68995938 MS.gene98177:CDS 25.0%
! TCTTTCGTCAATTCATATTT+CGG + chr8.2:68995966-68995985 None:intergenic 25.0%
AACGTTATATAATCCAAACC+AGG + chr8.2:68995918-68995937 None:intergenic 30.0%
ATGAATTGACGAAAGAAGAT+GGG - chr8.2:68995970-68995989 MS.gene98177:CDS 30.0%
GGTTTGGATTATATAACGTT+AGG - chr8.2:68995918-68995937 MS.gene98177:CDS 30.0%
GTATATTGAGAAGATTAAGG+AGG - chr8.2:68996193-68996212 MS.gene98177:CDS 30.0%
TATGAATTGACGAAAGAAGA+TGG - chr8.2:68995969-68995988 MS.gene98177:CDS 30.0%
TGGGTATATTGAGAAGATTA+AGG - chr8.2:68996190-68996209 MS.gene98177:CDS 30.0%
TTATATAACGTTAGGGTTAG+TGG - chr8.2:68995926-68995945 MS.gene98177:CDS 30.0%
TTCTCAATATACCCATCAAT+AGG + chr8.2:68996185-68996204 None:intergenic 30.0%
!! GAATCTTTTGAGGATGAAAA+AGG - chr8.2:68996061-68996080 MS.gene98177:CDS 30.0%
!!! GGTTTTTTTGACAGTGATTT+TGG + chr8.2:68995652-68995671 None:intergenic 30.0%
AAGCTGAACGAGAGAAATTA+GGG - chr8.2:68995799-68995818 MS.gene98177:CDS 35.0%
AGAAGAAAAACGCGAAGTTT+AGG - chr8.2:68995862-68995881 MS.gene98177:CDS 35.0%
ATAAACCTATTCACCGGAAA+CGG + chr8.2:68996146-68996165 None:intergenic 35.0%
GAAAGAAGATGGGATGAAAT+TGG - chr8.2:68995980-68995999 MS.gene98177:CDS 35.0%
GAAGAAAAACGCGAAGTTTA+GGG - chr8.2:68995863-68995882 MS.gene98177:CDS 35.0%
TCAATATACCCATCAATAGG+TGG + chr8.2:68996182-68996201 None:intergenic 35.0%
!! AGTATAGTAGGGTTTTGATG+AGG - chr8.2:68996009-68996028 MS.gene98177:CDS 35.0%
!! TCTTTTGAGGATGAAAAAGG+AGG - chr8.2:68996064-68996083 MS.gene98177:CDS 35.0%
!!! CAGTGATTTTGGAACGTTTT+AGG + chr8.2:68995641-68995660 None:intergenic 35.0%
GAAGCTGAACGAGAGAAATT+AGG - chr8.2:68995798-68995817 MS.gene98177:CDS 40.0%
GTTTCCGGTGAATAGGTTTA+TGG - chr8.2:68996145-68996164 MS.gene98177:CDS 40.0%
TCTGAGAAGCCAACGATAAT+GGG - chr8.2:68995888-68995907 MS.gene98177:CDS 40.0%
TTGAGAAAACGAAGTGGTGT+TGG + chr8.2:68995759-68995778 None:intergenic 40.0%
TTGGGTTTGAGAAAACGAAG+TGG + chr8.2:68995765-68995784 None:intergenic 40.0%
! AATTGGCGAAGGAGTATAGT+AGG - chr8.2:68995997-68996016 MS.gene98177:CDS 40.0%
! ATTGGCGAAGGAGTATAGTA+GGG - chr8.2:68995998-68996017 MS.gene98177:CDS 40.0%
! TTCTGAGAAGCCAACGATAA+TGG - chr8.2:68995887-68995906 MS.gene98177:CDS 40.0%
!! GGTTTTGATGAGGAAGCATA+GGG - chr8.2:68996019-68996038 MS.gene98177:CDS 40.0%
!!! GGAACGTTTTAGGATTTGAC+CGG + chr8.2:68995631-68995650 None:intergenic 40.0%
AGATGGGATGAAATTGGCGA+AGG - chr8.2:68995986-68996005 MS.gene98177:CDS 45.0%
AGATTAAGGAGGCTGCTGAT+AGG - chr8.2:68996204-68996223 MS.gene98177:CDS 45.0%
AGGCTATTGAAGCTTTGCCT+GGG - chr8.2:68996084-68996103 MS.gene98177:CDS 45.0%
GCCAAAGGAAAAGGAAAAGC+CGG - chr8.2:68995609-68995628 MS.gene98177:CDS 45.0%
GTCGCCATAAACCTATTCAC+CGG + chr8.2:68996152-68996171 None:intergenic 45.0%
TCAGTGGGAAGGAGAAGATT+AGG - chr8.2:68996228-68996247 MS.gene98177:CDS 45.0%
! GGCTTTTCCTTTTCCTTTGG+CGG + chr8.2:68995610-68995629 None:intergenic 45.0%
! GTTCACGTTCTTGTTTCTCG+CGG + chr8.2:68995710-68995729 None:intergenic 45.0%
! TTCACGTTCTTGTTTCTCGC+GGG + chr8.2:68995709-68995728 None:intergenic 45.0%
!! ACCGGCTTTTCCTTTTCCTT+TGG + chr8.2:68995613-68995632 None:intergenic 45.0%
!! GATAATGGGAACACCTGGTT+TGG - chr8.2:68995902-68995921 MS.gene98177:CDS 45.0%
!! GGGTTTTGATGAGGAAGCAT+AGG - chr8.2:68996018-68996037 MS.gene98177:CDS 45.0%
ACCGGAAACGGAGTCAAATC+CGG + chr8.2:68996134-68996153 None:intergenic 50.0%
ACGCTTACGCCACCTATTGA+TGG - chr8.2:68996170-68996189 MS.gene98177:CDS 50.0%
AGGCTGCTGATAGGATCAGT+GGG - chr8.2:68996213-68996232 MS.gene98177:CDS 50.0%
CCAGGTGTTCCCATTATCGT+TGG + chr8.2:68995900-68995919 None:intergenic 50.0%
CGCTTACGCCACCTATTGAT+GGG - chr8.2:68996171-68996190 MS.gene98177:CDS 50.0%
GAGGCTATTGAAGCTTTGCC+TGG - chr8.2:68996083-68996102 MS.gene98177:CDS 50.0%
GCTATTGAAGCTTTGCCTGG+GGG - chr8.2:68996086-68996105 MS.gene98177:CDS 50.0%
GGCTATTGAAGCTTTGCCTG+GGG - chr8.2:68996085-68996104 MS.gene98177:CDS 50.0%
GGGTTTGAAGGAAGCTGCTT+TGG - chr8.2:68996106-68996125 MS.gene98177:CDS 50.0%
GGTGAAGATGACAGTGGTGA+TGG + chr8.2:68995552-68995571 None:intergenic 50.0%
TCCGGATTTGACTCCGTTTC+CGG - chr8.2:68996130-68996149 MS.gene98177:CDS 50.0%
TGACTCCGTTTCCGGTGAAT+AGG - chr8.2:68996138-68996157 MS.gene98177:CDS 50.0%
TGCTGATAGGATCAGTGGGA+AGG - chr8.2:68996217-68996236 MS.gene98177:CDS 50.0%
TGGTGAGGTGAAGATGACAG+TGG + chr8.2:68995558-68995577 None:intergenic 50.0%
! GAAGGAAGCTGCTTTGGTTC+CGG - chr8.2:68996112-68996131 MS.gene98177:CDS 50.0%
! TTCCTTTTCCTTTGGCGGTG+CGG + chr8.2:68995605-68995624 None:intergenic 50.0%
!! CCAACGATAATGGGAACACC+TGG - chr8.2:68995897-68995916 MS.gene98177:CDS 50.0%
!! CGTTGAGGCATTGATCGTAG+AGG + chr8.2:68995734-68995753 None:intergenic 50.0%
!! GAAGATGACAGTGGTGATGG+TGG + chr8.2:68995549-68995568 None:intergenic 50.0%
AGCTTCGCGTGCACGTTGTT+TGG + chr8.2:68995784-68995803 None:intergenic 55.0%
AGCTTTGCCTGGGGGTTTGA+AGG - chr8.2:68996094-68996113 MS.gene98177:CDS 55.0%
GAAGCATAGGGCGAGACAAG+CGG - chr8.2:68996031-68996050 MS.gene98177:CDS 55.0%
GAGGCTGCTGATAGGATCAG+TGG - chr8.2:68996212-68996231 MS.gene98177:CDS 55.0%
GCTTCGCGTGCACGTTGTTT+GGG + chr8.2:68995783-68995802 None:intergenic 55.0%
GTTTCTCGCGGGAACCAGTT+GGG + chr8.2:68995698-68995717 None:intergenic 55.0%
TGTTTCTCGCGGGAACCAGT+TGG + chr8.2:68995699-68995718 None:intergenic 55.0%
TTTCTCGCGGGAACCAGTTG+GGG + chr8.2:68995697-68995716 None:intergenic 55.0%
! CGGCGGAGACGAATCTTTTG+AGG - chr8.2:68996051-68996070 MS.gene98177:CDS 55.0%
!! GAAGTGGTGTTGGTGCGTTG+AGG + chr8.2:68995749-68995768 None:intergenic 55.0%
AGCCGCACCGCCAAAGGAAA+AGG - chr8.2:68995600-68995619 MS.gene98177:CDS 60.0%
GCAGCTTCCTTCAAACCCCC+AGG + chr8.2:68996104-68996123 None:intergenic 60.0%
!! CAGCGTTGGAGGAGTTGGTG+AGG + chr8.2:68995573-68995592 None:intergenic 60.0%
ACCGGATCCGAACCCACACC+CGG - chr8.2:68995669-68995688 MS.gene98177:CDS 65.0%
GCATAGGGCGAGACAAGCGG+CGG - chr8.2:68996034-68996053 MS.gene98177:CDS 65.0%
TCCGGGTGTGGGTTCGGATC+CGG + chr8.2:68995673-68995692 None:intergenic 65.0%
TCCTTTGGCGGTGCGGCTGA+CGG + chr8.2:68995598-68995617 None:intergenic 65.0%
! CGGAGCAGCGTTGGAGGAGT+TGG + chr8.2:68995578-68995597 None:intergenic 65.0%
ACCAGTTGGGGCTCCGGGTG+TGG + chr8.2:68995685-68995704 None:intergenic 70.0%
CCAGTTGGGGCTCCGGGTGT+GGG + chr8.2:68995684-68995703 None:intergenic 70.0%
CGGGAACCAGTTGGGGCTCC+GGG + chr8.2:68995690-68995709 None:intergenic 70.0%
GCGGGAACCAGTTGGGGCTC+CGG + chr8.2:68995691-68995710 None:intergenic 70.0%
TCCGTCAGCCGCACCGCCAA+AGG - chr8.2:68995594-68995613 MS.gene98177:CDS 70.0%
TGCGGCTGACGGAGCAGCGT+TGG + chr8.2:68995587-68995606 None:intergenic 70.0%
TGGGGCTCCGGGTGTGGGTT+CGG + chr8.2:68995679-68995698 None:intergenic 70.0%
! GGCTGACGGAGCAGCGTTGG+AGG + chr8.2:68995584-68995603 None:intergenic 70.0%
CCCACACCCGGAGCCCCAAC+TGG - chr8.2:68995681-68995700 MS.gene98177:CDS 75.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 68995522 68996253 68995522 ID=MS.gene98177
chr8.2 mRNA 68995522 68996253 68995522 ID=MS.gene98177.t1;Parent=MS.gene98177
chr8.2 exon 68995522 68996253 68995522 ID=MS.gene98177.t1.exon1;Parent=MS.gene98177.t1
chr8.2 CDS 68995522 68996253 68995522 ID=cds.MS.gene98177.t1;Parent=MS.gene98177.t1
Gene Sequence

>MS.gene98177

ATGCTTCGCATCACAAAGCTAAAACCACCATCACCACTGTCATCTTCACCTCACCAACTCCTCCAACGCTGCTCCGTCAGCCGCACCGCCAAAGGAAAAGGAAAAGCCGGTCAAATCCTAAAACGTTCCAAAATCACTGTCAAAAAAACCGGATCCGAACCCACACCCGGAGCCCCAACTGGTTCCCGCGAGAAACAAGAACGTGAACGCCTCTACGATCAATGCCTCAACGCACCAACACCACTTCGTTTTCTCAAACCCAAACAACGTGCACGCGAAGCTGAACGAGAGAAATTAGGGTTAATCAGCAAAGATCGTCAAATTGAACTCGATATGATGAAGAAGAAAAACGCGAAGTTTAGGGTTTCTGAGAAGCCAACGATAATGGGAACACCTGGTTTGGATTATATAACGTTAGGGTTAGTGGATGCTGAGAAATTGCCGAAATATGAATTGACGAAAGAAGATGGGATGAAATTGGCGAAGGAGTATAGTAGGGTTTTGATGAGGAAGCATAGGGCGAGACAAGCGGCGGAGACGAATCTTTTGAGGATGAAAAAGGAGGCTATTGAAGCTTTGCCTGGGGGTTTGAAGGAAGCTGCTTTGGTTCCGGATTTGACTCCGTTTCCGGTGAATAGGTTTATGGCGACGCTTACGCCACCTATTGATGGGTATATTGAGAAGATTAAGGAGGCTGCTGATAGGATCAGTGGGAAGGAGAAGATTAGGTAG

Protein sequence

>MS.gene98177.t1

MLRITKLKPPSPLSSSPHQLLQRCSVSRTAKGKGKAGQILKRSKITVKKTGSEPTPGAPTGSREKQERERLYDQCLNAPTPLRFLKPKQRAREAEREKLGLISKDRQIELDMMKKKNAKFRVSEKPTIMGTPGLDYITLGLVDAEKLPKYELTKEDGMKLAKEYSRVLMRKHRARQAAETNLLRMKKEAIEALPGGLKEAALVPDLTPFPVNRFMATLTPPIDGYIEKIKEAADRISGKEKIR