Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80199.t1 | XP_003603305.1 | 93.9 | 495 | 30 | 0 | 1 | 495 | 1 | 495 | 4.20E-237 | 830.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80199.t1 | Q9C7G1 | 25.6 | 359 | 224 | 10 | 93 | 423 | 403 | 746 | 7.2e-20 | 100.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80199.t1 | G7J9V6 | 93.9 | 495 | 30 | 0 | 1 | 495 | 1 | 495 | 3.0e-237 | 830.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049190 | MS.gene80199 | 0.82454 | 7.64E-54 | -1.69E-46 |
| MS.gene049418 | MS.gene80199 | 0.840669 | 7.56E-58 | -1.69E-46 |
| MS.gene051021 | MS.gene80199 | 0.800594 | 1.34E-48 | -1.69E-46 |
| MS.gene051263 | MS.gene80199 | 0.812286 | 4.58E-51 | -1.69E-46 |
| MS.gene051648 | MS.gene80199 | 0.800992 | 1.11E-48 | -1.69E-46 |
| MS.gene053122 | MS.gene80199 | 0.802592 | 5.21E-49 | -1.69E-46 |
| MS.gene053144 | MS.gene80199 | 0.836564 | 8.69E-57 | -1.69E-46 |
| MS.gene054584 | MS.gene80199 | 0.839243 | 1.78E-57 | -1.69E-46 |
| MS.gene055537 | MS.gene80199 | 0.829931 | 3.91E-55 | -1.69E-46 |
| MS.gene055580 | MS.gene80199 | -0.810183 | 1.31E-50 | -1.69E-46 |
| MS.gene056169 | MS.gene80199 | 0.8113 | 7.50E-51 | -1.69E-46 |
| MS.gene056228 | MS.gene80199 | 0.832244 | 1.06E-55 | -1.69E-46 |
| MS.gene057082 | MS.gene80199 | 0.831495 | 1.62E-55 | -1.69E-46 |
| MS.gene058197 | MS.gene80199 | 0.81937 | 1.20E-52 | -1.69E-46 |
| MS.gene058198 | MS.gene80199 | 0.811627 | 6.37E-51 | -1.69E-46 |
| MS.gene05894 | MS.gene80199 | 0.812038 | 5.18E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80199.t1 | MTR_3g106140 | 97.172 | 495 | 14 | 0 | 1 | 495 | 1 | 495 | 0.0 | 980 |
| MS.gene80199.t1 | MTR_1g014420 | 63.985 | 522 | 154 | 7 | 1 | 495 | 1 | 515 | 0.0 | 545 |
| MS.gene80199.t1 | MTR_2g015870 | 32.124 | 386 | 231 | 6 | 110 | 495 | 86 | 440 | 9.98e-42 | 154 |
| MS.gene80199.t1 | MTR_8g080280 | 31.290 | 310 | 192 | 8 | 95 | 394 | 411 | 709 | 1.99e-29 | 122 |
| MS.gene80199.t1 | MTR_4g485520 | 30.094 | 319 | 207 | 7 | 79 | 394 | 174 | 479 | 1.39e-26 | 112 |
| MS.gene80199.t1 | MTR_4g485520 | 30.094 | 319 | 207 | 7 | 79 | 394 | 342 | 647 | 1.97e-26 | 113 |
| MS.gene80199.t1 | MTR_4g063800 | 31.488 | 289 | 178 | 8 | 108 | 391 | 351 | 624 | 2.09e-26 | 113 |
| MS.gene80199.t1 | MTR_5g079550 | 32.721 | 272 | 166 | 6 | 128 | 394 | 38 | 297 | 2.86e-26 | 110 |
| MS.gene80199.t1 | MTR_5g069340 | 34.799 | 273 | 161 | 5 | 128 | 394 | 52 | 313 | 9.57e-25 | 105 |
| MS.gene80199.t1 | MTR_1g018180 | 28.814 | 295 | 196 | 6 | 110 | 402 | 61 | 343 | 1.41e-24 | 105 |
| MS.gene80199.t1 | MTR_1g069845 | 30.137 | 292 | 188 | 7 | 107 | 394 | 317 | 596 | 6.85e-24 | 105 |
| MS.gene80199.t1 | MTR_1g069845 | 30.137 | 292 | 188 | 7 | 107 | 394 | 438 | 717 | 8.80e-24 | 105 |
| MS.gene80199.t1 | MTR_1g069845 | 30.137 | 292 | 188 | 7 | 107 | 394 | 434 | 713 | 8.92e-24 | 105 |
| MS.gene80199.t1 | MTR_3g466220 | 31.834 | 289 | 180 | 7 | 109 | 394 | 531 | 805 | 1.23e-23 | 105 |
| MS.gene80199.t1 | MTR_3g466220 | 31.834 | 289 | 180 | 7 | 109 | 394 | 552 | 826 | 1.62e-23 | 104 |
| MS.gene80199.t1 | MTR_7g059405 | 30.526 | 285 | 186 | 5 | 107 | 391 | 358 | 630 | 2.31e-23 | 103 |
| MS.gene80199.t1 | MTR_3g085610 | 29.221 | 308 | 197 | 8 | 96 | 394 | 412 | 707 | 8.26e-23 | 102 |
| MS.gene80199.t1 | MTR_7g097020 | 28.618 | 304 | 200 | 8 | 105 | 404 | 474 | 764 | 1.03e-22 | 102 |
| MS.gene80199.t1 | MTR_1g094215 | 31.973 | 294 | 186 | 6 | 108 | 399 | 160 | 441 | 3.99e-22 | 99.4 |
| MS.gene80199.t1 | MTR_3g067685 | 29.714 | 350 | 210 | 9 | 126 | 469 | 56 | 375 | 5.33e-22 | 98.2 |
| MS.gene80199.t1 | MTR_7g078330 | 31.250 | 288 | 184 | 6 | 105 | 390 | 362 | 637 | 6.57e-22 | 99.8 |
| MS.gene80199.t1 | MTR_1g094215 | 31.973 | 294 | 186 | 6 | 108 | 399 | 343 | 624 | 1.29e-21 | 98.6 |
| MS.gene80199.t1 | MTR_6g004790 | 30.822 | 292 | 181 | 8 | 111 | 394 | 3 | 281 | 3.05e-19 | 89.0 |
| MS.gene80199.t1 | MTR_3g103050 | 27.063 | 303 | 201 | 9 | 110 | 405 | 51 | 340 | 1.28e-17 | 84.3 |
| MS.gene80199.t1 | MTR_3g078340 | 26.299 | 308 | 208 | 6 | 89 | 394 | 196 | 486 | 2.28e-16 | 82.0 |
| MS.gene80199.t1 | MTR_2g026130 | 27.517 | 298 | 202 | 6 | 99 | 394 | 164 | 449 | 1.13e-15 | 79.7 |
| MS.gene80199.t1 | MTR_2g018010 | 26.102 | 295 | 186 | 8 | 113 | 394 | 399 | 674 | 1.63e-13 | 73.6 |
| MS.gene80199.t1 | MTR_5g083030 | 27.444 | 266 | 164 | 6 | 89 | 334 | 367 | 623 | 1.00e-12 | 70.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene80199.t1 | AT4G31890 | 60.300 | 534 | 157 | 13 | 1 | 495 | 1 | 518 | 0.0 | 553 |
| MS.gene80199.t1 | AT4G31890 | 60.300 | 534 | 157 | 13 | 1 | 495 | 1 | 518 | 0.0 | 553 |
| MS.gene80199.t1 | AT4G31890 | 60.300 | 534 | 157 | 13 | 1 | 495 | 1 | 518 | 0.0 | 553 |
| MS.gene80199.t1 | AT2G25130 | 58.696 | 506 | 160 | 10 | 1 | 495 | 1 | 468 | 5.53e-179 | 510 |
| MS.gene80199.t1 | AT2G27430 | 31.877 | 389 | 233 | 8 | 110 | 495 | 79 | 438 | 1.22e-41 | 154 |
| MS.gene80199.t1 | AT2G23140 | 29.250 | 400 | 244 | 11 | 15 | 394 | 247 | 627 | 1.52e-30 | 125 |
| MS.gene80199.t1 | AT2G23140 | 29.250 | 400 | 244 | 11 | 15 | 394 | 247 | 627 | 1.52e-30 | 125 |
| MS.gene80199.t1 | AT2G23140 | 29.250 | 400 | 244 | 11 | 15 | 394 | 439 | 819 | 2.69e-30 | 125 |
| MS.gene80199.t1 | AT2G23140 | 29.250 | 400 | 244 | 11 | 15 | 394 | 436 | 816 | 3.05e-30 | 125 |
| MS.gene80199.t1 | AT2G23140 | 29.250 | 400 | 244 | 11 | 15 | 394 | 436 | 816 | 3.05e-30 | 125 |
| MS.gene80199.t1 | AT1G67530 | 30.142 | 282 | 177 | 6 | 124 | 397 | 440 | 709 | 5.77e-27 | 115 |
| MS.gene80199.t1 | AT1G67530 | 30.142 | 282 | 177 | 6 | 124 | 397 | 440 | 709 | 5.77e-27 | 115 |
| MS.gene80199.t1 | AT1G67530 | 30.142 | 282 | 177 | 6 | 124 | 397 | 440 | 709 | 5.77e-27 | 115 |
| MS.gene80199.t1 | AT1G24330 | 29.316 | 307 | 200 | 6 | 118 | 418 | 434 | 729 | 1.81e-25 | 110 |
| MS.gene80199.t1 | AT1G24330 | 29.316 | 307 | 200 | 6 | 118 | 418 | 434 | 729 | 1.81e-25 | 110 |
| MS.gene80199.t1 | AT3G46510 | 33.088 | 272 | 162 | 8 | 125 | 391 | 369 | 625 | 2.20e-25 | 110 |
| MS.gene80199.t1 | AT3G54790 | 30.282 | 284 | 184 | 7 | 113 | 394 | 441 | 712 | 3.04e-25 | 110 |
| MS.gene80199.t1 | AT5G67340 | 29.653 | 317 | 207 | 7 | 79 | 394 | 396 | 697 | 3.52e-25 | 109 |
| MS.gene80199.t1 | AT5G67340 | 29.653 | 317 | 207 | 7 | 79 | 394 | 396 | 697 | 3.52e-25 | 109 |
| MS.gene80199.t1 | AT3G54790 | 30.068 | 296 | 192 | 8 | 113 | 405 | 477 | 760 | 4.50e-25 | 109 |
| MS.gene80199.t1 | AT3G54790 | 30.068 | 296 | 192 | 8 | 113 | 405 | 477 | 760 | 4.50e-25 | 109 |
| MS.gene80199.t1 | AT2G28830 | 31.023 | 303 | 187 | 10 | 109 | 403 | 284 | 572 | 1.41e-24 | 107 |
| MS.gene80199.t1 | AT3G03440 | 26.829 | 369 | 231 | 10 | 110 | 470 | 66 | 403 | 2.08e-23 | 102 |
| MS.gene80199.t1 | AT1G23030 | 29.514 | 288 | 188 | 6 | 110 | 394 | 333 | 608 | 2.15e-22 | 101 |
| MS.gene80199.t1 | AT1G27910 | 25.627 | 359 | 224 | 10 | 93 | 423 | 403 | 746 | 9.42e-22 | 99.4 |
| MS.gene80199.t1 | AT1G71020 | 30.822 | 292 | 186 | 7 | 107 | 394 | 192 | 471 | 2.09e-21 | 97.4 |
| MS.gene80199.t1 | AT1G71020 | 30.822 | 292 | 186 | 7 | 107 | 394 | 340 | 619 | 2.82e-21 | 97.8 |
| MS.gene80199.t1 | AT3G01400 | 27.152 | 302 | 200 | 8 | 110 | 408 | 64 | 348 | 3.40e-20 | 92.4 |
| MS.gene80199.t1 | AT5G58680 | 29.963 | 267 | 166 | 7 | 124 | 388 | 77 | 324 | 1.08e-19 | 90.9 |
| MS.gene80199.t1 | AT3G54850 | 31.802 | 283 | 179 | 6 | 122 | 402 | 359 | 629 | 1.34e-19 | 92.4 |
| MS.gene80199.t1 | AT4G12710 | 30.928 | 291 | 184 | 5 | 112 | 394 | 49 | 330 | 1.46e-18 | 88.2 |
| MS.gene80199.t1 | AT5G42340 | 30.263 | 304 | 198 | 6 | 90 | 391 | 361 | 652 | 2.47e-18 | 88.6 |
| MS.gene80199.t1 | AT5G65200 | 29.044 | 272 | 174 | 6 | 132 | 394 | 248 | 509 | 9.65e-18 | 86.7 |
| MS.gene80199.t1 | AT4G16490 | 28.333 | 300 | 199 | 7 | 99 | 394 | 174 | 461 | 1.34e-16 | 82.8 |
| MS.gene80199.t1 | AT5G14510 | 30.075 | 266 | 170 | 8 | 135 | 394 | 24 | 279 | 3.99e-16 | 79.7 |
| MS.gene80199.t1 | AT5G62560 | 26.298 | 289 | 200 | 5 | 109 | 394 | 235 | 513 | 4.18e-15 | 78.2 |
Find 94 sgRNAs with CRISPR-Local
Find 111 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAAGGATGCAATCACAATTT+TGG | 0.171187 | 3.4:-92572304 | MS.gene80199:CDS |
| GATTCTAACAAACCAATAAT+TGG | 0.187713 | 3.4:-92572591 | MS.gene80199:CDS |
| GATAAAGGGAAGCAGGTTTC+TGG | 0.201909 | 3.4:-92572081 | MS.gene80199:CDS |
| GACGAATATTTCATTTGTTT+TGG | 0.222706 | 3.4:-92572448 | MS.gene80199:CDS |
| GCACCAGAGGATCCAATTAT+TGG | 0.223709 | 3.4:+92572579 | None:intergenic |
| AAAGCAGCGATTGTTAAAAT+TGG | 0.251431 | 3.4:-92572714 | MS.gene80199:CDS |
| GAAGCCTTCTCTTGACATTC+AGG | 0.257804 | 3.4:+92572255 | None:intergenic |
| CCTTTCAATTCTGAGCAATT+TGG | 0.260209 | 3.4:-92572364 | MS.gene80199:CDS |
| TCAACTGCAATTCGTCAAAA+TGG | 0.295980 | 3.4:+92571787 | None:intergenic |
| AGGAGGTAAAGAGTGTTTAA+TGG | 0.305362 | 3.4:-92571977 | MS.gene80199:CDS |
| AAAAGCTATTAGTGCTGTTA+AGG | 0.376170 | 3.4:-92572322 | MS.gene80199:CDS |
| GGCGGAGAAGCGGAAGTTAC+CGG | 0.380244 | 3.4:+92573433 | None:intergenic |
| TTTCCTTGGATTAAGTGCTT+TGG | 0.382622 | 3.4:-92572613 | MS.gene80199:CDS |
| AAGTTAAACAAGATGCTCTT+CGG | 0.386930 | 3.4:-92572497 | MS.gene80199:CDS |
| GAAGACGAAGAAGCACAAAT+AGG | 0.399369 | 3.4:+92572018 | None:intergenic |
| AGAGGCTCCGCCGCAGCTTA+AGG | 0.401079 | 3.4:+92573382 | None:intergenic |
| GCAATTGTTGCAAATTTCCT+TGG | 0.402106 | 3.4:-92572627 | MS.gene80199:CDS |
| GAGGCACACTCGCAATGCTC+GGG | 0.403694 | 3.4:-92573079 | MS.gene80199:CDS |
| GGGCAATTTCTCCTCTCGTT+GGG | 0.404224 | 3.4:-92573058 | MS.gene80199:CDS |
| AGAGAAGACGGAGAAGCTAT+TGG | 0.413753 | 3.4:-92573270 | MS.gene80199:CDS |
| GGAGATGAAGCATGTGGTTA+AGG | 0.413838 | 3.4:-92573183 | MS.gene80199:CDS |
| CACATAAAGCTTATGCTGAT+AGG | 0.414828 | 3.4:-92572212 | MS.gene80199:CDS |
| TACAATTCCTGCTTCAATCA+TGG | 0.419654 | 3.4:+92572185 | None:intergenic |
| TGCGAGTGTGCCTCTCGCTT+CGG | 0.424046 | 3.4:+92573088 | None:intergenic |
| TTGCTTGAGCTGACGCTTGT+GGG | 0.429303 | 3.4:-92572156 | MS.gene80199:CDS |
| CAGAGCTAATTTCTTCTTCA+CGG | 0.429412 | 3.4:+92573339 | None:intergenic |
| AGAAGTGATGGTGGAAACTT+AGG | 0.433937 | 3.4:-92572057 | MS.gene80199:CDS |
| ATAAAGGGAAGCAGGTTTCT+GGG | 0.440773 | 3.4:-92572080 | MS.gene80199:CDS |
| GGGGCAATTTCTCCTCTCGT+TGG | 0.443059 | 3.4:-92573059 | MS.gene80199:CDS |
| GACGAAGCGGCGAATGGCAG+CGG | 0.449732 | 3.4:-92573141 | MS.gene80199:CDS |
| AGTTAAACAAGATGCTCTTC+GGG | 0.453105 | 3.4:-92572496 | MS.gene80199:CDS |
| ACCTTGTGTTGTTGATTCAT+CGG | 0.459932 | 3.4:-92572658 | MS.gene80199:CDS |
| AAACCAATAATTGGATCCTC+TGG | 0.464190 | 3.4:-92572582 | MS.gene80199:CDS |
| TTCTCCGCGTAACTCCTTCA+CGG | 0.469664 | 3.4:+92573301 | None:intergenic |
| TAGTGCCTTGGCACAGAAAA+GGG | 0.470454 | 3.4:-92572133 | MS.gene80199:CDS |
| AGAGGCACACTCGCAATGCT+CGG | 0.474592 | 3.4:-92573080 | MS.gene80199:CDS |
| CTATATGCTTTACTCAATCT+CGG | 0.476166 | 3.4:-92572996 | MS.gene80199:CDS |
| TAAAGAGTGTTTAATGGAGG+AGG | 0.476887 | 3.4:-92571971 | MS.gene80199:CDS |
| GAAGAAGGAAGAGACATTAA+CGG | 0.484776 | 3.4:-92573204 | MS.gene80199:CDS |
| TAGGCTAGATAAAGGGAAGC+AGG | 0.493141 | 3.4:-92572088 | MS.gene80199:CDS |
| ATTGGATCTTCTGAACATAC+AGG | 0.495508 | 3.4:-92573252 | MS.gene80199:CDS |
| GACGCTTGTGGGTAGTGCCT+TGG | 0.497097 | 3.4:-92572145 | MS.gene80199:CDS |
| TTTCAGAGTTCTAACTAGGA+AGG | 0.499120 | 3.4:+92572551 | None:intergenic |
| CAATTGAATCAACGTTGCTC+CGG | 0.501968 | 3.4:+92573492 | None:intergenic |
| GTAGTGCCTTGGCACAGAAA+AGG | 0.502811 | 3.4:-92572134 | MS.gene80199:CDS |
| AATGATGCGGCGGAAGGATG+AGG | 0.503235 | 3.4:+92573412 | None:intergenic |
| ATTGCTTGAGCTGACGCTTG+TGG | 0.515359 | 3.4:-92572157 | MS.gene80199:CDS |
| CTTCTTCACGGTGGTAATGT+CGG | 0.521877 | 3.4:+92573351 | None:intergenic |
| TTCAGAGTTCTAACTAGGAA+GGG | 0.526621 | 3.4:+92572552 | None:intergenic |
| GAGAGAAACTTACGCATCGT+TGG | 0.530546 | 3.4:+92572968 | None:intergenic |
| AGGTAAAGAGTGTTTAATGG+AGG | 0.535851 | 3.4:-92571974 | MS.gene80199:CDS |
| AATTAGCTCTGTCGCGTCGA+CGG | 0.541624 | 3.4:-92573327 | MS.gene80199:CDS |
| GGAAGGATGAGGCGGAGAAG+CGG | 0.545683 | 3.4:+92573423 | None:intergenic |
| AGGCAAGCCATGATTGAAGC+AGG | 0.546829 | 3.4:-92572192 | MS.gene80199:CDS |
| CAGGTTTCTGGGAGAAGTGA+TGG | 0.551186 | 3.4:-92572069 | MS.gene80199:CDS |
| GTCGAAAATGATGCGGCGGA+AGG | 0.563722 | 3.4:+92573406 | None:intergenic |
| AGAAGTGCGAGAAGAGAAGA+CGG | 0.565520 | 3.4:-92573282 | MS.gene80199:CDS |
| CCAAATTGCTCAGAATTGAA+AGG | 0.568365 | 3.4:+92572364 | None:intergenic |
| TGTTGATTCATCGGTTTCTG+AGG | 0.570602 | 3.4:-92572649 | MS.gene80199:CDS |
| AAATGCGGAATCGAAGAAGA+AGG | 0.572172 | 3.4:-92573219 | MS.gene80199:CDS |
| CATGATGAAAATTGTCAAGA+GGG | 0.572944 | 3.4:-92571884 | MS.gene80199:CDS |
| CGCGTCGACGGCAACCGTGA+AGG | 0.579924 | 3.4:-92573315 | MS.gene80199:CDS |
| TGGCACAGAAAAGGGCATCA+AGG | 0.580100 | 3.4:-92572125 | MS.gene80199:CDS |
| CTGCAATTCGTCAAAATGGA+AGG | 0.580211 | 3.4:+92571791 | None:intergenic |
| GAGAAATGGCTAAGTGTCAC+CGG | 0.581139 | 3.4:-92573511 | None:intergenic |
| AGTGAAGCAAAACGCTCGGA+AGG | 0.587883 | 3.4:+92571838 | None:intergenic |
| AGACTCGACGAAGCGGCGAA+TGG | 0.588292 | 3.4:-92573147 | MS.gene80199:CDS |
| AATCCAAAGCACTTAATCCA+AGG | 0.592090 | 3.4:+92572610 | None:intergenic |
| TTCACCTGAATGTCAAGAGA+AGG | 0.606603 | 3.4:-92572259 | MS.gene80199:CDS |
| TTCGACGCCTTAAGCTGCGG+CGG | 0.610282 | 3.4:-92573389 | MS.gene80199:CDS |
| AGTGAGTGAAGCAAAACGCT+CGG | 0.610988 | 3.4:+92571834 | None:intergenic |
| CAATTTGGTGTCGAGTCCCG+AGG | 0.611390 | 3.4:-92572349 | MS.gene80199:CDS |
| ACATGATGAAAATTGTCAAG+AGG | 0.618956 | 3.4:-92571885 | MS.gene80199:CDS |
| TAGGAAGGGTATTGCACCAG+AGG | 0.641343 | 3.4:+92572566 | None:intergenic |
| GCGGAGAAGACTCGACGAAG+CGG | 0.643214 | 3.4:-92573154 | MS.gene80199:CDS |
| GTAATGTCGGTGGTGTCGAG+AGG | 0.644001 | 3.4:+92573364 | None:intergenic |
| GATAAACTCGATAGAAGACA+TGG | 0.646492 | 3.4:-92572403 | MS.gene80199:CDS |
| AAAGAAGATTCCGAAGCGAG+AGG | 0.648370 | 3.4:-92573098 | MS.gene80199:CDS |
| CTTCACGGTGGTAATGTCGG+TGG | 0.649189 | 3.4:+92573354 | None:intergenic |
| AGGCACACTCGCAATGCTCG+GGG | 0.651896 | 3.4:-92573078 | MS.gene80199:CDS |
| GTTTCTGGGAGAAGTGATGG+TGG | 0.653879 | 3.4:-92572066 | MS.gene80199:CDS |
| ACCGATGAATCAACAACACA+AGG | 0.656484 | 3.4:+92572657 | None:intergenic |
| AATTTGGTGTCGAGTCCCGA+GGG | 0.656713 | 3.4:-92572348 | MS.gene80199:CDS |
| GATGCGGCGGAAGGATGAGG+CGG | 0.658978 | 3.4:+92573415 | None:intergenic |
| TTTGTGAAGACAGATGGAGG+AGG | 0.662270 | 3.4:-92571994 | MS.gene80199:CDS |
| GAATCAAGCATCCCAACGAG+AGG | 0.667890 | 3.4:+92573047 | None:intergenic |
| AGCTAATTTCTTCTTCACGG+TGG | 0.668202 | 3.4:+92573342 | None:intergenic |
| CATGTGGTTAAGGATTTACG+CGG | 0.683685 | 3.4:-92573173 | MS.gene80199:CDS |
| ACAGGTTCATGAAACAAATG+CGG | 0.698371 | 3.4:-92573234 | MS.gene80199:CDS |
| AGGCGTCGAAAATGATGCGG+CGG | 0.698501 | 3.4:+92573402 | None:intergenic |
| TTAAGGCGTCGAAAATGATG+CGG | 0.708242 | 3.4:+92573399 | None:intergenic |
| GGAGAAGCGGAAGTTACCGG+CGG | 0.717102 | 3.4:+92573436 | None:intergenic |
| ATTAACGGAGATGAAGCATG+TGG | 0.719445 | 3.4:-92573189 | MS.gene80199:CDS |
| GCAACCGTGAAGGAGTTACG+CGG | 0.747970 | 3.4:-92573305 | MS.gene80199:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATCATATTCTTAATTTTG+TGG | - | chr3.4:92572461-92572480 | MS.gene80199:CDS | 15.0% |
| !! | GAATATGATAAATTCATGAT+TGG | + | chr3.4:92572452-92572471 | None:intergenic | 20.0% |
| !! | GATTAAACACAAAAATTTCA+CGG | + | chr3.4:92572541-92572560 | None:intergenic | 20.0% |
| !!! | TTAATCTTTATTTGTTTCTG+TGG | - | chr3.4:92572555-92572574 | MS.gene80199:CDS | 20.0% |
| !!! | TTCATACATTTTGATGATTA+TGG | - | chr3.4:92573072-92573091 | MS.gene80199:CDS | 20.0% |
| ! | GATTCTAACAAACCAATAAT+TGG | - | chr3.4:92572716-92572735 | MS.gene80199:CDS | 25.0% |
| !! | GACGAATATTTCATTTGTTT+TGG | - | chr3.4:92572859-92572878 | MS.gene80199:intron | 25.0% |
| !!! | AATGTTTTAGGCTAGATAAA+GGG | - | chr3.4:92573212-92573231 | MS.gene80199:CDS | 25.0% |
| !!! | AATTTTTCAGAGTTCTAACT+AGG | + | chr3.4:92572763-92572782 | None:intergenic | 25.0% |
| !!! | ATTTGTTTTGGAAACTGATA+TGG | - | chr3.4:92572871-92572890 | MS.gene80199:intron | 25.0% |
| !!! | TTTTGGTTGATGTATTGAAT+TGG | - | chr3.4:92573020-92573039 | MS.gene80199:CDS | 25.0% |
| AAAGCAGCGATTGTTAAAAT+TGG | - | chr3.4:92572593-92572612 | MS.gene80199:CDS | 30.0% | |
| AAGTTAAACAAGATGCTCTT+CGG | - | chr3.4:92572810-92572829 | MS.gene80199:intron | 30.0% | |
| ACATGATGAAAATTGTCAAG+AGG | - | chr3.4:92573422-92573441 | MS.gene80199:CDS | 30.0% | |
| CATGATGAAAATTGTCAAGA+GGG | - | chr3.4:92573423-92573442 | MS.gene80199:CDS | 30.0% | |
| CTATATGCTTTACTCAATCT+CGG | - | chr3.4:92572311-92572330 | MS.gene80199:CDS | 30.0% | |
| ! | AAAAGCTATTAGTGCTGTTA+AGG | - | chr3.4:92572985-92573004 | MS.gene80199:CDS | 30.0% |
| ! | CAAGGATATTGCAATGTTTT+AGG | - | chr3.4:92573200-92573219 | MS.gene80199:CDS | 30.0% |
| ! | TAAGGATGCAATCACAATTT+TGG | - | chr3.4:92573003-92573022 | MS.gene80199:CDS | 30.0% |
| !! | CAATGTTTTAGGCTAGATAA+AGG | - | chr3.4:92573211-92573230 | MS.gene80199:CDS | 30.0% |
| AAACCAATAATTGGATCCTC+TGG | - | chr3.4:92572725-92572744 | MS.gene80199:intron | 35.0% | |
| ACAGGTTCATGAAACAAATG+CGG | - | chr3.4:92572073-92572092 | MS.gene80199:CDS | 35.0% | |
| AGTTAAACAAGATGCTCTTC+GGG | - | chr3.4:92572811-92572830 | MS.gene80199:intron | 35.0% | |
| ATTGGATCTTCTGAACATAC+AGG | - | chr3.4:92572055-92572074 | MS.gene80199:CDS | 35.0% | |
| CACATAAAGCTTATGCTGAT+AGG | - | chr3.4:92573095-92573114 | MS.gene80199:CDS | 35.0% | |
| CAGAGCTAATTTCTTCTTCA+CGG | + | chr3.4:92571971-92571990 | None:intergenic | 35.0% | |
| CCAAATTGCTCAGAATTGAA+AGG | + | chr3.4:92572946-92572965 | None:intergenic | 35.0% | |
| CCTTTCAATTCTGAGCAATT+TGG | - | chr3.4:92572943-92572962 | MS.gene80199:intron | 35.0% | |
| GAAGAAGGAAGAGACATTAA+CGG | - | chr3.4:92572103-92572122 | MS.gene80199:CDS | 35.0% | |
| GATAAACTCGATAGAAGACA+TGG | - | chr3.4:92572904-92572923 | MS.gene80199:intron | 35.0% | |
| GCAATTGTTGCAAATTTCCT+TGG | - | chr3.4:92572680-92572699 | MS.gene80199:CDS | 35.0% | |
| TACAATTCCTGCTTCAATCA+TGG | + | chr3.4:92573125-92573144 | None:intergenic | 35.0% | |
| TTTCCTTGGATTAAGTGCTT+TGG | - | chr3.4:92572694-92572713 | MS.gene80199:CDS | 35.0% | |
| ! | AATCCAAAGCACTTAATCCA+AGG | + | chr3.4:92572700-92572719 | None:intergenic | 35.0% |
| ! | ACTAATAGCTTTTCTACCCT+CGG | + | chr3.4:92572978-92572997 | None:intergenic | 35.0% |
| ! | TCTTCTTTTGTGAAGACAGA+TGG | - | chr3.4:92573307-92573326 | MS.gene80199:CDS | 35.0% |
| ! | TTCAGAGTTCTAACTAGGAA+GGG | + | chr3.4:92572758-92572777 | None:intergenic | 35.0% |
| ! | TTTCAGAGTTCTAACTAGGA+AGG | + | chr3.4:92572759-92572778 | None:intergenic | 35.0% |
| !! | ACCTTGTGTTGTTGATTCAT+CGG | - | chr3.4:92572649-92572668 | MS.gene80199:CDS | 35.0% |
| !! | AGGAGGTAAAGAGTGTTTAA+TGG | - | chr3.4:92573330-92573349 | MS.gene80199:CDS | 35.0% |
| !! | AGGTAAAGAGTGTTTAATGG+AGG | - | chr3.4:92573333-92573352 | MS.gene80199:CDS | 35.0% |
| !! | TAAAGAGTGTTTAATGGAGG+AGG | - | chr3.4:92573336-92573355 | MS.gene80199:CDS | 35.0% |
| AAATGCGGAATCGAAGAAGA+AGG | - | chr3.4:92572088-92572107 | MS.gene80199:CDS | 40.0% | |
| ACCGATGAATCAACAACACA+AGG | + | chr3.4:92572653-92572672 | None:intergenic | 40.0% | |
| AGCTAATTTCTTCTTCACGG+TGG | + | chr3.4:92571968-92571987 | None:intergenic | 40.0% | |
| ATAAAGGGAAGCAGGTTTCT+GGG | - | chr3.4:92573227-92573246 | MS.gene80199:CDS | 40.0% | |
| ATTAACGGAGATGAAGCATG+TGG | - | chr3.4:92572118-92572137 | MS.gene80199:CDS | 40.0% | |
| CAATTGAATCAACGTTGCTC+CGG | + | chr3.4:92571818-92571837 | None:intergenic | 40.0% | |
| CATGTGGTTAAGGATTTACG+CGG | - | chr3.4:92572134-92572153 | MS.gene80199:CDS | 40.0% | |
| TTCACCTGAATGTCAAGAGA+AGG | - | chr3.4:92573048-92573067 | MS.gene80199:CDS | 40.0% | |
| ! | CTAATAGCTTTTCTACCCTC+GGG | + | chr3.4:92572977-92572996 | None:intergenic | 40.0% |
| ! | GAAGACGAAGAAGCACAAAT+AGG | + | chr3.4:92573292-92573311 | None:intergenic | 40.0% |
| ! | TCTTTTGTGAAGACAGATGG+AGG | - | chr3.4:92573310-92573329 | MS.gene80199:CDS | 40.0% |
| !! | AGAAGTGATGGTGGAAACTT+AGG | - | chr3.4:92573250-92573269 | MS.gene80199:CDS | 40.0% |
| !! | TGTTGATTCATCGGTTTCTG+AGG | - | chr3.4:92572658-92572677 | MS.gene80199:CDS | 40.0% |
| !! | TTAAGGCGTCGAAAATGATG+CGG | + | chr3.4:92571911-92571930 | None:intergenic | 40.0% |
| AAAGAAGATTCCGAAGCGAG+AGG | - | chr3.4:92572209-92572228 | MS.gene80199:CDS | 45.0% | |
| AGAGAAGACGGAGAAGCTAT+TGG | - | chr3.4:92572037-92572056 | MS.gene80199:CDS | 45.0% | |
| AGTGAGTGAAGCAAAACGCT+CGG | + | chr3.4:92573476-92573495 | None:intergenic | 45.0% | |
| GAAGCCTTCTCTTGACATTC+AGG | + | chr3.4:92573055-92573074 | None:intergenic | 45.0% | |
| GAGAGAAACTTACGCATCGT+TGG | + | chr3.4:92572342-92572361 | None:intergenic | 45.0% | |
| GATAAAGGGAAGCAGGTTTC+TGG | - | chr3.4:92573226-92573245 | MS.gene80199:CDS | 45.0% | |
| GCACCAGAGGATCCAATTAT+TGG | + | chr3.4:92572731-92572750 | None:intergenic | 45.0% | |
| GGAGATGAAGCATGTGGTTA+AGG | - | chr3.4:92572124-92572143 | MS.gene80199:CDS | 45.0% | |
| TAGGCTAGATAAAGGGAAGC+AGG | - | chr3.4:92573219-92573238 | MS.gene80199:CDS | 45.0% | |
| TAGTGCCTTGGCACAGAAAA+GGG | - | chr3.4:92573174-92573193 | MS.gene80199:CDS | 45.0% | |
| TTTGTGAAGACAGATGGAGG+AGG | - | chr3.4:92573313-92573332 | MS.gene80199:CDS | 45.0% | |
| ! | AGAAGTGCGAGAAGAGAAGA+CGG | - | chr3.4:92572025-92572044 | MS.gene80199:CDS | 45.0% |
| ! | ATTTTCGACGCCTTAAGCTG+CGG | - | chr3.4:92571915-92571934 | MS.gene80199:CDS | 45.0% |
| ! | CTTCTTCACGGTGGTAATGT+CGG | + | chr3.4:92571959-92571978 | None:intergenic | 45.0% |
| AGGCAAGCCATGATTGAAGC+AGG | - | chr3.4:92573115-92573134 | MS.gene80199:CDS | 50.0% | |
| AGTGAAGCAAAACGCTCGGA+AGG | + | chr3.4:92573472-92573491 | None:intergenic | 50.0% | |
| ATTGCTTGAGCTGACGCTTG+TGG | - | chr3.4:92573150-92573169 | MS.gene80199:CDS | 50.0% | |
| CATGCGAAAAACACTACCGC+CGG | - | chr3.4:92571855-92571874 | MS.gene80199:CDS | 50.0% | |
| GAATCAAGCATCCCAACGAG+AGG | + | chr3.4:92572263-92572282 | None:intergenic | 50.0% | |
| GGGCAATTTCTCCTCTCGTT+GGG | - | chr3.4:92572249-92572268 | MS.gene80199:CDS | 50.0% | |
| GTAGTGCCTTGGCACAGAAA+AGG | - | chr3.4:92573173-92573192 | MS.gene80199:CDS | 50.0% | |
| TTCTCCGCGTAACTCCTTCA+CGG | + | chr3.4:92572009-92572028 | None:intergenic | 50.0% | |
| TTGCTTGAGCTGACGCTTGT+GGG | - | chr3.4:92573151-92573170 | MS.gene80199:CDS | 50.0% | |
| ! | CAGGTTTCTGGGAGAAGTGA+TGG | - | chr3.4:92573238-92573257 | MS.gene80199:CDS | 50.0% |
| ! | CGGTAGTGTTTTTCGCATGG+TGG | + | chr3.4:92571854-92571873 | None:intergenic | 50.0% |
| ! | GTTTCTGGGAGAAGTGATGG+TGG | - | chr3.4:92573241-92573260 | MS.gene80199:CDS | 50.0% |
| ! | TGATGCCCTTTTCTGTGCCA+AGG | + | chr3.4:92573182-92573201 | None:intergenic | 50.0% |
| !! | AATTAGCTCTGTCGCGTCGA+CGG | - | chr3.4:92571980-92571999 | MS.gene80199:CDS | 50.0% |
| !! | AATTTGGTGTCGAGTCCCGA+GGG | - | chr3.4:92572959-92572978 | MS.gene80199:intron | 50.0% |
| !! | TAGGAAGGGTATTGCACCAG+AGG | + | chr3.4:92572744-92572763 | None:intergenic | 50.0% |
| !! | TGGCACAGAAAAGGGCATCA+AGG | - | chr3.4:92573182-92573201 | MS.gene80199:CDS | 50.0% |
| AATGATGCGGCGGAAGGATG+AGG | + | chr3.4:92571898-92571917 | None:intergenic | 55.0% | |
| GCAACCGTGAAGGAGTTACG+CGG | - | chr3.4:92572002-92572021 | MS.gene80199:CDS | 55.0% | |
| GGGGCAATTTCTCCTCTCGT+TGG | - | chr3.4:92572248-92572267 | MS.gene80199:CDS | 55.0% | |
| GTCGAAAATGATGCGGCGGA+AGG | + | chr3.4:92571904-92571923 | None:intergenic | 55.0% | |
| ! | AGAGGCACACTCGCAATGCT+CGG | - | chr3.4:92572227-92572246 | MS.gene80199:CDS | 55.0% |
| ! | CGGCGGTAGTGTTTTTCGCA+TGG | + | chr3.4:92571857-92571876 | None:intergenic | 55.0% |
| ! | CTTCACGGTGGTAATGTCGG+TGG | + | chr3.4:92571956-92571975 | None:intergenic | 55.0% |
| ! | GTAATGTCGGTGGTGTCGAG+AGG | + | chr3.4:92571946-92571965 | None:intergenic | 55.0% |
| !! | AGGCGTCGAAAATGATGCGG+CGG | + | chr3.4:92571908-92571927 | None:intergenic | 55.0% |
| !! | CAATTTGGTGTCGAGTCCCG+AGG | - | chr3.4:92572958-92572977 | MS.gene80199:intron | 55.0% |
| AGACTCGACGAAGCGGCGAA+TGG | - | chr3.4:92572160-92572179 | MS.gene80199:CDS | 60.0% | |
| AGGCACACTCGCAATGCTCG+GGG | - | chr3.4:92572229-92572248 | MS.gene80199:CDS | 60.0% | |
| GACGCTTGTGGGTAGTGCCT+TGG | - | chr3.4:92573162-92573181 | MS.gene80199:CDS | 60.0% | |
| GAGGCACACTCGCAATGCTC+GGG | - | chr3.4:92572228-92572247 | MS.gene80199:CDS | 60.0% | |
| GCGGAGAAGACTCGACGAAG+CGG | - | chr3.4:92572153-92572172 | MS.gene80199:CDS | 60.0% | |
| GGAAGGATGAGGCGGAGAAG+CGG | + | chr3.4:92571887-92571906 | None:intergenic | 60.0% | |
| GGAGAAGCGGAAGTTACCGG+CGG | + | chr3.4:92571874-92571893 | None:intergenic | 60.0% | |
| GGCGGAGAAGCGGAAGTTAC+CGG | + | chr3.4:92571877-92571896 | None:intergenic | 60.0% | |
| TGCGAGTGTGCCTCTCGCTT+CGG | + | chr3.4:92572222-92572241 | None:intergenic | 60.0% | |
| TTCGACGCCTTAAGCTGCGG+CGG | - | chr3.4:92571918-92571937 | MS.gene80199:CDS | 60.0% | |
| AGAGGCTCCGCCGCAGCTTA+AGG | + | chr3.4:92571928-92571947 | None:intergenic | 65.0% | |
| GACGAAGCGGCGAATGGCAG+CGG | - | chr3.4:92572166-92572185 | MS.gene80199:CDS | 65.0% | |
| GATGCGGCGGAAGGATGAGG+CGG | + | chr3.4:92571895-92571914 | None:intergenic | 65.0% | |
| ! | CGCGTCGACGGCAACCGTGA+AGG | - | chr3.4:92571992-92572011 | MS.gene80199:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 92571801 | 92573528 | 92571801 | ID=MS.gene80199 |
| chr3.4 | mRNA | 92571801 | 92573528 | 92571801 | ID=MS.gene80199.t1;Parent=MS.gene80199 |
| chr3.4 | exon | 92572981 | 92573528 | 92572981 | ID=MS.gene80199.t1.exon1;Parent=MS.gene80199.t1 |
| chr3.4 | CDS | 92572981 | 92573528 | 92572981 | ID=cds.MS.gene80199.t1;Parent=MS.gene80199.t1 |
| chr3.4 | exon | 92571801 | 92572740 | 92571801 | ID=MS.gene80199.t1.exon2;Parent=MS.gene80199.t1 |
| chr3.4 | CDS | 92571801 | 92572740 | 92571801 | ID=cds.MS.gene80199.t1;Parent=MS.gene80199.t1 |
| Gene Sequence |
| Protein sequence |
