Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81695.t1 | XP_003616794.1 | 75.2 | 133 | 5 | 2 | 1 | 105 | 1 | 133 | 4.40E-19 | 104 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81695.t1 | G7KH64 | 75.2 | 133 | 5 | 2 | 1 | 105 | 1 | 133 | 3.1e-19 | 104.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050020 | MS.gene81695 | 0.810308 | 1.23E-50 | -1.69E-46 |
| MS.gene050563 | MS.gene81695 | 0.808277 | 3.35E-50 | -1.69E-46 |
| MS.gene050584 | MS.gene81695 | 0.857313 | 1.78E-62 | -1.69E-46 |
| MS.gene050585 | MS.gene81695 | 0.828041 | 1.12E-54 | -1.69E-46 |
| MS.gene050587 | MS.gene81695 | 0.833062 | 6.62E-56 | -1.69E-46 |
| MS.gene05143 | MS.gene81695 | 0.809901 | 1.50E-50 | -1.69E-46 |
| MS.gene051789 | MS.gene81695 | 0.860832 | 1.57E-63 | -1.69E-46 |
| MS.gene052142 | MS.gene81695 | 0.829114 | 6.17E-55 | -1.69E-46 |
| MS.gene053074 | MS.gene81695 | 0.808794 | 2.60E-50 | -1.69E-46 |
| MS.gene053383 | MS.gene81695 | 0.817728 | 2.84E-52 | -1.69E-46 |
| MS.gene05456 | MS.gene81695 | 0.857603 | 1.46E-62 | -1.69E-46 |
| MS.gene055359 | MS.gene81695 | 0.814285 | 1.67E-51 | -1.69E-46 |
| MS.gene059006 | MS.gene81695 | 0.864691 | 1.01E-64 | -1.69E-46 |
| MS.gene060691 | MS.gene81695 | 0.821595 | 3.72E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene81695.t1 | MTR_5g084330 | 86.598 | 97 | 10 | 2 | 11 | 105 | 38 | 133 | 1.80e-23 | 87.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 41 sgRNAs with CRISPR-Local
Find 72 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAATTAAATGATGGCAAATA+TGG | 0.218116 | 5.1:-66379655 | MS.gene81695:CDS |
| CCTGGTCGTGGTGGTAATTA+TGG | 0.320779 | 5.1:-66379613 | MS.gene81695:CDS |
| TAATTACCACCACGGTTTCC+AGG | 0.361900 | 5.1:+66379520 | None:intergenic |
| TGTTGCGGTGATAGGTATTA+TGG | 0.365471 | 5.1:-66379484 | MS.gene81695:CDS |
| TAATTACCACCACGGTTTCC+AGG | 0.368625 | 5.1:+66379556 | None:intergenic |
| TAACCACCACCACGGTTTCC+AGG | 0.406140 | 5.1:+66379580 | None:intergenic |
| GCTATGGCTGTTGCGGTGAT+AGG | 0.407613 | 5.1:-66379492 | MS.gene81695:CDS |
| GGAAACCGTGGTGGTAATTA+CGG | 0.422488 | 5.1:-66379553 | MS.gene81695:CDS |
| AATTATGGTGGTGGTTATCC+TGG | 0.457834 | 5.1:-66379598 | MS.gene81695:CDS |
| GGCAAATATGGAAGCTATGG+TGG | 0.472000 | 5.1:-66379643 | MS.gene81695:CDS |
| CAAACAAATGAATTAAATGA+TGG | 0.499941 | 5.1:-66379664 | MS.gene81695:CDS |
| GTTGCGGTGATAGGTATTAT+GGG | 0.502063 | 5.1:-66379483 | MS.gene81695:CDS |
| TAGCCAATAAACCTAGGATA+AGG | 0.503132 | 5.1:+66380332 | None:intergenic |
| GCAAATATGGAAGCTATGGT+GGG | 0.509000 | 5.1:-66379642 | MS.gene81695:CDS |
| AATTACCACCACGGTTTCCA+GGG | 0.523853 | 5.1:+66379521 | None:intergenic |
| CGTGGTGGTAATTATGGTGG+TGG | 0.530246 | 5.1:-66379607 | MS.gene81695:CDS |
| AATTACCACCACGGTTTCCA+GGG | 0.540342 | 5.1:+66379557 | None:intergenic |
| GATGGCAAATATGGAAGCTA+TGG | 0.545533 | 5.1:-66379646 | MS.gene81695:CDS |
| AATTACGGTGGTGGTTACCC+TGG | 0.550608 | 5.1:-66379538 | MS.gene81695:CDS |
| AACCGTGGTGGTGGTTACCC+TGG | 0.559819 | 5.1:-66379574 | MS.gene81695:CDS |
| GGTATTATGGGAGTTGCAGA+AGG | 0.562319 | 5.1:-66379471 | MS.gene81695:CDS |
| AGTGTTGTTCTCAGTTTGCA+CGG | 0.568848 | 5.1:+66379420 | None:intergenic |
| CGTGGTGGTAATTACGGTGG+TGG | 0.570111 | 5.1:-66379547 | MS.gene81695:CDS |
| GGTGGTAATTATTGTCGCTA+TGG | 0.572979 | 5.1:-66379508 | MS.gene81695:CDS |
| TATCCTGGAAACCGTGGTGG+TGG | 0.578604 | 5.1:-66379583 | MS.gene81695:CDS |
| CCATAATTACCACCACGACC+AGG | 0.597203 | 5.1:+66379613 | None:intergenic |
| TATTGTCGCTATGGCTGTTG+CGG | 0.598460 | 5.1:-66379499 | MS.gene81695:CDS |
| CAAATATGGAAGCTATGGTG+GGG | 0.613749 | 5.1:-66379641 | MS.gene81695:CDS |
| AGAAGGTGTTGCTCATATGC+TGG | 0.617122 | 5.1:-66379454 | MS.gene81695:CDS |
| AAATATGGAAGCTATGGTGG+GGG | 0.641886 | 5.1:-66379640 | MS.gene81695:CDS |
| GGTCGTGGTGGTAATTATGG+TGG | 0.644801 | 5.1:-66379610 | MS.gene81695:CDS |
| CACCACCGTAATTACCACCA+CGG | 0.655753 | 5.1:+66379548 | None:intergenic |
| GGTGGTTACCCTGGAAACCG+TGG | 0.656654 | 5.1:-66379529 | MS.gene81695:CDS |
| TTCCAGGGTAACCACCACCA+CGG | 0.661772 | 5.1:+66379572 | None:intergenic |
| GGTTATCCTGGAAACCGTGG+TGG | 0.662961 | 5.1:-66379586 | MS.gene81695:CDS |
| GGTGGTTACCCTGGAAACCG+TGG | 0.668990 | 5.1:-66379565 | MS.gene81695:CDS |
| GGTGGTTATCCTGGAAACCG+TGG | 0.690182 | 5.1:-66379589 | MS.gene81695:CDS |
| AGCGACAATAATTACCACCA+CGG | 0.696105 | 5.1:+66379512 | None:intergenic |
| AACCGTGGTGGTAATTACGG+TGG | 0.699420 | 5.1:-66379550 | MS.gene81695:CDS |
| GGTTACCCTGGAAACCGTGG+TGG | 0.745370 | 5.1:-66379526 | MS.gene81695:CDS |
| GGTTACCCTGGAAACCGTGG+TGG | 0.745370 | 5.1:-66379562 | MS.gene81695:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTCTTTTTAAAAATCACTT+GGG | - | chr5.1:66379833-66379852 | MS.gene81695:intron | 15.0% |
| !!! | ATTTATATTTTGGATCAATT+TGG | + | chr5.1:66379693-66379712 | None:intergenic | 15.0% |
| !!! | TATTCTTTTTAAAAATCACT+TGG | - | chr5.1:66379832-66379851 | MS.gene81695:intron | 15.0% |
| !! | CAAACAAATGAATTAAATGA+TGG | - | chr5.1:66380099-66380118 | MS.gene81695:intron | 20.0% |
| !!! | TAAACAAACTCACATTTTAA+TGG | + | chr5.1:66379968-66379987 | None:intergenic | 20.0% |
| ! | GAATTAAATGATGGCAAATA+TGG | - | chr5.1:66380108-66380127 | MS.gene81695:intron | 25.0% |
| AAAAATGCAATCCTTATCCT+AGG | - | chr5.1:66379420-66379439 | MS.gene81695:CDS | 30.0% | |
| AATCCTTATCCTAGGTTTAT+TGG | - | chr5.1:66379428-66379447 | MS.gene81695:CDS | 30.0% | |
| CCAAGAAATATAGCTTCTAA+CGG | + | chr5.1:66379643-66379662 | None:intergenic | 30.0% | |
| CGTTAGAAGCTATATTTCTT+GGG | - | chr5.1:66379641-66379660 | MS.gene81695:CDS | 30.0% | |
| GTTAGAAGCTATATTTCTTG+GGG | - | chr5.1:66379642-66379661 | MS.gene81695:CDS | 30.0% | |
| ! | TTTGAAACGTATCATGTAAC+CGG | + | chr5.1:66379607-66379626 | None:intergenic | 30.0% |
| !! | ACAAACTCACATTTTAATGG+TGG | + | chr5.1:66379965-66379984 | None:intergenic | 30.0% |
| !! | TCTGAAGGCATTATTATCAA+AGG | + | chr5.1:66379580-66379599 | None:intergenic | 30.0% |
| AATAATGCCTTCAGAAATGC+CGG | - | chr5.1:66379585-66379604 | MS.gene81695:CDS | 35.0% | |
| ACATCCTCTAATACCCAAAA+AGG | - | chr5.1:66379501-66379520 | MS.gene81695:CDS | 35.0% | |
| AGAAATATAGCTTCTAACGG+TGG | + | chr5.1:66379640-66379659 | None:intergenic | 35.0% | |
| CCGTTAGAAGCTATATTTCT+TGG | - | chr5.1:66379640-66379659 | MS.gene81695:CDS | 35.0% | |
| CGAATACTATTAGATGTGCA+TGG | - | chr5.1:66379808-66379827 | MS.gene81695:intron | 35.0% | |
| GAATGATAGCCAATAAACCT+AGG | + | chr5.1:66379440-66379459 | None:intergenic | 35.0% | |
| TAGCCAATAAACCTAGGATA+AGG | + | chr5.1:66379434-66379453 | None:intergenic | 35.0% | |
| TCATATCTCCTCTGATGTTT+CGG | - | chr5.1:66379461-66379480 | MS.gene81695:CDS | 35.0% | |
| ! | AATGTACAATCTCGCCTTTT+TGG | + | chr5.1:66379518-66379537 | None:intergenic | 35.0% |
| ! | ATGTACAATCTCGCCTTTTT+GGG | + | chr5.1:66379517-66379536 | None:intergenic | 35.0% |
| AAATATGGAAGCTATGGTGG+GGG | - | chr5.1:66380123-66380142 | MS.gene81695:intron | 40.0% | |
| AGCGACAATAATTACCACCA+CGG | + | chr5.1:66380254-66380273 | None:intergenic | 40.0% | |
| CAAATATGGAAGCTATGGTG+GGG | - | chr5.1:66380122-66380141 | MS.gene81695:intron | 40.0% | |
| CATACGTAATGTGTTTGCAG+AGG | - | chr5.1:66380068-66380087 | MS.gene81695:intron | 40.0% | |
| GATGGCAAATATGGAAGCTA+TGG | - | chr5.1:66380117-66380136 | MS.gene81695:intron | 40.0% | |
| GCAAATATGGAAGCTATGGT+GGG | - | chr5.1:66380121-66380140 | MS.gene81695:intron | 40.0% | |
| GGTGGTAATTATTGTCGCTA+TGG | - | chr5.1:66380255-66380274 | MS.gene81695:intron | 40.0% | |
| GTTGCGGTGATAGGTATTAT+GGG | - | chr5.1:66380280-66380299 | MS.gene81695:intron | 40.0% | |
| TGTTGCGGTGATAGGTATTA+TGG | - | chr5.1:66380279-66380298 | MS.gene81695:intron | 40.0% | |
| ! | AGTGTTGTTCTCAGTTTGCA+CGG | + | chr5.1:66380346-66380365 | None:intergenic | 40.0% |
| !! | AATTATGGTGGTGGTTATCC+TGG | - | chr5.1:66380165-66380184 | MS.gene81695:intron | 40.0% |
| AATTACCACCACGGTTTCCA+GGG | + | chr5.1:66380209-66380228 | None:intergenic | 45.0% | |
| ACTGTGTTAGTGTTGCAGTG+TGG | + | chr5.1:66379904-66379923 | None:intergenic | 45.0% | |
| CATGTAACCGGCATTTCTGA+AGG | + | chr5.1:66379595-66379614 | None:intergenic | 45.0% | |
| GGAAACCGTGGTGGTAATTA+CGG | - | chr5.1:66380210-66380229 | MS.gene81695:intron | 45.0% | |
| GGCAAATATGGAAGCTATGG+TGG | - | chr5.1:66380120-66380139 | MS.gene81695:intron | 45.0% | |
| GGTATTATGGGAGTTGCAGA+AGG | - | chr5.1:66380292-66380311 | MS.gene81695:intron | 45.0% | |
| TAATTACCACCACGGTTTCC+AGG | + | chr5.1:66380210-66380229 | None:intergenic | 45.0% | |
| TATTGTCGCTATGGCTGTTG+CGG | - | chr5.1:66380264-66380283 | MS.gene81695:intron | 45.0% | |
| !! | AGAAGGTGTTGCTCATATGC+TGG | - | chr5.1:66380309-66380328 | MS.gene81695:intron | 45.0% |
| !! | TGCAGTGTGGATCGATTTTG+AGG | + | chr5.1:66379891-66379910 | None:intergenic | 45.0% |
| !!! | CTCGCCTTTTTGGGTATTAG+AGG | + | chr5.1:66379508-66379527 | None:intergenic | 45.0% |
| !!! | AATAAAATCAATTTATATTT+TGG | + | chr5.1:66379703-66379722 | None:intergenic | 5.0% |
| !!! | AATTGAATTTTATAATTAAT+AGG | - | chr5.1:66380044-66380063 | MS.gene81695:intron | 5.0% |
| !!! | ATTGAATTTTATAATTAATA+GGG | - | chr5.1:66380045-66380064 | MS.gene81695:intron | 5.0% |
| AACCGTGGTGGTAATTACGG+TGG | - | chr5.1:66380213-66380232 | MS.gene81695:intron | 50.0% | |
| CACCACCGTAATTACCACCA+CGG | + | chr5.1:66380218-66380237 | None:intergenic | 50.0% | |
| CCATAATTACCACCACGACC+AGG | + | chr5.1:66380153-66380172 | None:intergenic | 50.0% | |
| CCTGGTCGTGGTGGTAATTA+TGG | - | chr5.1:66380150-66380169 | MS.gene81695:intron | 50.0% | |
| CGTGGTGGTAATTATGGTGG+TGG | - | chr5.1:66380156-66380175 | MS.gene81695:intron | 50.0% | |
| CTCCTCTGATGTTTCGGCTA+GGG | - | chr5.1:66379467-66379486 | MS.gene81695:CDS | 50.0% | |
| GGTCGTGGTGGTAATTATGG+TGG | - | chr5.1:66380153-66380172 | MS.gene81695:intron | 50.0% | |
| TCTCCTCTGATGTTTCGGCT+AGG | - | chr5.1:66379466-66379485 | MS.gene81695:CDS | 50.0% | |
| ! | AATTACGGTGGTGGTTACCC+TGG | - | chr5.1:66380225-66380244 | MS.gene81695:intron | 50.0% |
| CGTGGTGGTAATTACGGTGG+TGG | - | chr5.1:66380216-66380235 | MS.gene81695:intron | 55.0% | |
| GCTATGGCTGTTGCGGTGAT+AGG | - | chr5.1:66380271-66380290 | MS.gene81695:intron | 55.0% | |
| GGTGGTTATCCTGGAAACCG+TGG | - | chr5.1:66380174-66380193 | MS.gene81695:intron | 55.0% | |
| GGTTATCCTGGAAACCGTGG+TGG | - | chr5.1:66380177-66380196 | MS.gene81695:intron | 55.0% | |
| GTCCCTAGCCGAAACATCAG+AGG | + | chr5.1:66379472-66379491 | None:intergenic | 55.0% | |
| TAACCACCACCACGGTTTCC+AGG | + | chr5.1:66380186-66380205 | None:intergenic | 55.0% | |
| TATCCTGGAAACCGTGGTGG+TGG | - | chr5.1:66380180-66380199 | MS.gene81695:intron | 55.0% | |
| TTCCAGGGTAACCACCACCA+CGG | + | chr5.1:66380194-66380213 | None:intergenic | 55.0% | |
| GGTGGTTACCCTGGAAACCG+TGG | - | chr5.1:66380198-66380217 | MS.gene81695:intron | 60.0% | |
| GGTTACCCTGGAAACCGTGG+TGG | - | chr5.1:66380201-66380220 | MS.gene81695:intron | 60.0% | |
| ! | AGCTATGGTGGGGGCTATCC+TGG | - | chr5.1:66380132-66380151 | MS.gene81695:intron | 60.0% |
| !! | AACCGTGGTGGTGGTTACCC+TGG | - | chr5.1:66380189-66380208 | MS.gene81695:intron | 60.0% |
| GGGGGCTATCCTGGTCGTGG+TGG | - | chr5.1:66380141-66380160 | MS.gene81695:intron | 70.0% | |
| GGTGGGGGCTATCCTGGTCG+TGG | - | chr5.1:66380138-66380157 | MS.gene81695:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 66379411 | 66380374 | 66379411 | ID=MS.gene81695 |
| chr5.1 | mRNA | 66379411 | 66380374 | 66379411 | ID=MS.gene81695.t1;Parent=MS.gene81695 |
| chr5.1 | exon | 66380344 | 66380374 | 66380344 | ID=MS.gene81695.t1.exon1;Parent=MS.gene81695.t1 |
| chr5.1 | CDS | 66380344 | 66380374 | 66380344 | ID=cds.MS.gene81695.t1;Parent=MS.gene81695.t1 |
| chr5.1 | exon | 66379411 | 66379697 | 66379411 | ID=MS.gene81695.t1.exon2;Parent=MS.gene81695.t1 |
| chr5.1 | CDS | 66379411 | 66379697 | 66379411 | ID=cds.MS.gene81695.t1;Parent=MS.gene81695.t1 |
| Gene Sequence |
| Protein sequence |
