Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82097.t1 | XP_003619836.1 | 98.2 | 488 | 9 | 0 | 10 | 497 | 10 | 497 | 1.80E-285 | 991.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82097.t1 | Q9M571 | 74.3 | 483 | 124 | 0 | 14 | 496 | 11 | 493 | 1.4e-217 | 756.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82097.t1 | G7KPT7 | 98.2 | 488 | 9 | 0 | 10 | 497 | 10 | 497 | 1.3e-285 | 991.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056942 | MS.gene82097 | 0.800902 | 1.16E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene047006 | MS.gene82097 | PPI |
| MS.gene82097 | MS.gene066974 | PPI |
| MS.gene047043 | MS.gene82097 | PPI |
| MS.gene82097 | MS.gene042859 | PPI |
| MS.gene82097 | MS.gene040109 | PPI |
| MS.gene93748 | MS.gene82097 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82097.t1 | MTR_6g069600 | 98.156 | 488 | 9 | 0 | 10 | 497 | 10 | 497 | 0.0 | 1002 |
| MS.gene82097.t1 | MTR_8g107370 | 80.992 | 484 | 91 | 1 | 13 | 496 | 5 | 487 | 0.0 | 801 |
| MS.gene82097.t1 | MTR_8g107370 | 80.992 | 484 | 91 | 1 | 13 | 496 | 11 | 493 | 0.0 | 801 |
| MS.gene82097.t1 | MTR_8g107370 | 80.973 | 452 | 85 | 1 | 13 | 464 | 11 | 461 | 0.0 | 747 |
| MS.gene82097.t1 | MTR_8g107370 | 81.967 | 427 | 76 | 1 | 13 | 439 | 11 | 436 | 0.0 | 716 |
| MS.gene82097.t1 | MTR_1g014690 | 28.302 | 159 | 102 | 4 | 290 | 436 | 75 | 233 | 8.73e-13 | 69.3 |
| MS.gene82097.t1 | MTR_1g014690 | 33.019 | 106 | 69 | 1 | 290 | 393 | 130 | 235 | 1.31e-12 | 69.3 |
| MS.gene82097.t1 | MTR_3g032530 | 31.034 | 116 | 78 | 1 | 280 | 393 | 43 | 158 | 1.24e-11 | 65.9 |
| MS.gene82097.t1 | MTR_3g032530 | 31.034 | 116 | 78 | 1 | 280 | 393 | 86 | 201 | 1.35e-11 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82097.t1 | AT1G73600 | 71.694 | 484 | 137 | 0 | 13 | 496 | 20 | 503 | 0.0 | 752 |
| MS.gene82097.t1 | AT1G73600 | 71.694 | 484 | 137 | 0 | 13 | 496 | 6 | 489 | 0.0 | 751 |
| MS.gene82097.t1 | AT3G18000 | 71.340 | 485 | 139 | 0 | 12 | 496 | 6 | 490 | 0.0 | 744 |
| MS.gene82097.t1 | AT1G48600 | 71.811 | 486 | 137 | 0 | 12 | 497 | 6 | 491 | 0.0 | 744 |
| MS.gene82097.t1 | AT1G48600 | 72.363 | 474 | 131 | 0 | 24 | 497 | 2 | 475 | 0.0 | 728 |
| MS.gene82097.t1 | AT5G13710 | 29.697 | 165 | 103 | 4 | 259 | 411 | 55 | 218 | 3.54e-13 | 71.2 |
| MS.gene82097.t1 | AT5G13710 | 29.697 | 165 | 103 | 4 | 259 | 411 | 55 | 218 | 3.54e-13 | 71.2 |
| MS.gene82097.t1 | AT1G64970 | 29.808 | 104 | 71 | 1 | 292 | 393 | 130 | 233 | 3.07e-11 | 65.5 |
Find 81 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACTCTATGAACATGTTTA+TGG | 0.176357 | 6.1:+63553207 | MS.gene82097:CDS |
| TGCTGTTGATCAAACCAATT+TGG | 0.240782 | 6.1:+63554618 | MS.gene82097:CDS |
| TTGATTTGATATTCTCAAAT+TGG | 0.248443 | 6.1:+63552030 | MS.gene82097:CDS |
| AGCTAGAGAAATCATGTTTA+TGG | 0.260404 | 6.1:-63553724 | None:intergenic |
| TCCTTTGAGCTTTCTCTTGT+GGG | 0.294619 | 6.1:+63552933 | MS.gene82097:CDS |
| GTGTAGCTGGTGAGCAGATA+TGG | 0.296797 | 6.1:+63555329 | MS.gene82097:CDS |
| CTAGATGTTGGTTGTGGTAT+TGG | 0.308873 | 6.1:+63553642 | MS.gene82097:CDS |
| ACATGAACTTGACATTCTTA+TGG | 0.327278 | 6.1:-63551962 | None:intergenic |
| TGGAAGATTTACAGCTGAAT+TGG | 0.333381 | 6.1:+63551568 | MS.gene82097:CDS |
| TCATGCAGATTTGTTGGATA+TGG | 0.335290 | 6.1:+63553109 | MS.gene82097:intron |
| TTGCAGAATGAAAATACTAA+TGG | 0.336708 | 6.1:+63551936 | MS.gene82097:intron |
| AATACAATCCAACTCACTAT+AGG | 0.347505 | 6.1:+63552433 | MS.gene82097:CDS |
| GAATCTTGTTTCCACCAATC+TGG | 0.358429 | 6.1:+63552396 | MS.gene82097:CDS |
| CCTTGAGAAATATGCAAGTT+TGG | 0.359226 | 6.1:-63552002 | None:intergenic |
| TCTTTGTCATGCAGATTTGT+TGG | 0.362351 | 6.1:+63553103 | MS.gene82097:intron |
| GTTAAATCACAAGATGATAG+AGG | 0.369519 | 6.1:+63553138 | MS.gene82097:CDS |
| GTCTCTCTTCTTTGTCGAGA+TGG | 0.371915 | 6.1:-63542233 | None:intergenic |
| CTCCTTTGAGCTTTCTCTTG+TGG | 0.372126 | 6.1:+63552932 | MS.gene82097:CDS |
| ATACAATCCAACTCACTATA+GGG | 0.384922 | 6.1:+63552434 | MS.gene82097:CDS |
| AGGGAAATTGTGGCAAAGTT+AGG | 0.389582 | 6.1:+63553597 | MS.gene82097:CDS |
| GAGTGTGTGACAAACCAAAT+TGG | 0.399779 | 6.1:-63554632 | None:intergenic |
| TTAGAGCTTGGAGCAGGTAT+TGG | 0.405980 | 6.1:+63551548 | MS.gene82097:CDS |
| CAAACCTTAGTGTAAAATCT+TGG | 0.408255 | 6.1:-63552459 | None:intergenic |
| GTTCTTGAAAACTCTACAAA+TGG | 0.411827 | 6.1:+63554725 | MS.gene82097:intron |
| GTCAAATGGTTAAATGTTGA+TGG | 0.412539 | 6.1:+63552357 | MS.gene82097:CDS |
| TGACATGAAAGCATATTATC+AGG | 0.414876 | 6.1:+63554110 | MS.gene82097:CDS |
| GTGAAGTGCAAAGAAGTTAC+TGG | 0.416072 | 6.1:+63542154 | MS.gene82097:CDS |
| TCTCTCTTCTTTGTCGAGAT+GGG | 0.418673 | 6.1:-63542232 | None:intergenic |
| TCACAGATGCTTGAGAATGC+TGG | 0.438992 | 6.1:+63554580 | MS.gene82097:intron |
| TTCTCTTTGAATCTCCAGAT+TGG | 0.440419 | 6.1:-63552410 | None:intergenic |
| TGTCATATGAGTGATGATAA+TGG | 0.457058 | 6.1:+63552906 | MS.gene82097:CDS |
| TTTGTGAGCACAGGAGGACT+TGG | 0.457900 | 6.1:+63553237 | MS.gene82097:CDS |
| TGTAGCTGGTGAGCAGATAT+GGG | 0.458742 | 6.1:+63555330 | MS.gene82097:CDS |
| TGAAGTCCACAGCAAGCAAT+TGG | 0.460367 | 6.1:-63551604 | None:intergenic |
| GTCATATGAGTGATGATAAT+GGG | 0.461100 | 6.1:+63552907 | MS.gene82097:CDS |
| TAGCTGAAAGGATGGTCAAA+TGG | 0.462289 | 6.1:+63552343 | MS.gene82097:CDS |
| ATAATGAAATTGTTGAAAGA+TGG | 0.474872 | 6.1:+63555287 | MS.gene82097:CDS |
| TCTTTGAATCTCCAGATTGG+TGG | 0.475051 | 6.1:-63552407 | None:intergenic |
| AAATAAGAAGAATCAAAATC+AGG | 0.476569 | 6.1:+63552983 | MS.gene82097:CDS |
| GCTGAATTGGCTCAGAAAGC+TGG | 0.494705 | 6.1:+63551581 | MS.gene82097:CDS |
| GGCAAAGTTAGGACTAAAGC+CGG | 0.498474 | 6.1:+63553608 | MS.gene82097:CDS |
| CTTGGTTCCCTATAGTGAGT+TGG | 0.500725 | 6.1:-63552441 | None:intergenic |
| TATGAACATGTTTATGGTCA+AGG | 0.501805 | 6.1:+63553213 | MS.gene82097:CDS |
| GTTGGTTGTGGTATTGGAGG+TGG | 0.503392 | 6.1:+63553648 | MS.gene82097:CDS |
| CTTGCATATTTCTCAAGGAT+CGG | 0.508578 | 6.1:+63552007 | MS.gene82097:CDS |
| TCTCTATTACCAGCATATGA+AGG | 0.509429 | 6.1:+63551515 | MS.gene82097:CDS |
| GATAATATGCTTTCATGTCA+TGG | 0.509905 | 6.1:-63554107 | None:intergenic |
| AATCAACCTTCTTGTTCTCA+AGG | 0.512925 | 6.1:-63554758 | None:intergenic |
| CATAGAGTAAGATATCTTTG+TGG | 0.519852 | 6.1:-63553194 | None:intergenic |
| ACTGACTATTGCAAAAGTGT+TGG | 0.520445 | 6.1:+63554033 | MS.gene82097:CDS |
| AAGCAGATGAGAGGTGTAGC+TGG | 0.525626 | 6.1:+63555316 | MS.gene82097:CDS |
| GATGTTGGTTGTGGTATTGG+AGG | 0.526855 | 6.1:+63553645 | MS.gene82097:CDS |
| CATGTATCTTTCAGATGAAG+AGG | 0.527858 | 6.1:+63552058 | MS.gene82097:CDS |
| CCAAACTTGCATATTTCTCA+AGG | 0.528360 | 6.1:+63552002 | MS.gene82097:CDS |
| GGTCAAGGCTTTGTGAGCAC+AGG | 0.534562 | 6.1:+63553228 | MS.gene82097:CDS |
| CATTGAGAGTGCAATAAAGA+AGG | 0.534730 | 6.1:+63551625 | MS.gene82097:CDS |
| TAAAATGATCTGAACAATGT+TGG | 0.535832 | 6.1:-63553976 | None:intergenic |
| CCTTACTTTGATGTGTAACA+AGG | 0.537248 | 6.1:-63553853 | None:intergenic |
| TACTGCATATTTGAGTCCGA+TGG | 0.541796 | 6.1:-63553757 | None:intergenic |
| GTAATTAAAAGTGTACCTCC+AGG | 0.542732 | 6.1:-63554012 | None:intergenic |
| AGCTGGCCAATTGCTTGCTG+TGG | 0.543614 | 6.1:+63551598 | MS.gene82097:CDS |
| CTCTTCTTTGTCGAGATGGG+AGG | 0.545903 | 6.1:-63542229 | None:intergenic |
| CTTGTGGGATGCAAATGCAT+TGG | 0.550546 | 6.1:+63552948 | MS.gene82097:CDS |
| CAAGGCTTTGTGAGCACAGG+AGG | 0.555847 | 6.1:+63553231 | MS.gene82097:CDS |
| ATCAACCTTCTTGTTCTCAA+GGG | 0.563447 | 6.1:-63554757 | None:intergenic |
| TTAAATCACAAGATGATAGA+GGG | 0.564358 | 6.1:+63553139 | MS.gene82097:CDS |
| AGAAACAACAAGGGAAATTG+TGG | 0.570096 | 6.1:+63553587 | MS.gene82097:intron |
| ATTCTTGAGCAGAAACAACA+AGG | 0.575658 | 6.1:+63553577 | MS.gene82097:intron |
| AAGATGATAGAGGGTTTCAA+AGG | 0.577951 | 6.1:+63553148 | MS.gene82097:CDS |
| TGAAATTGTTGAAAGATGGA+AGG | 0.578969 | 6.1:+63555291 | MS.gene82097:CDS |
| AAATGCCCTTGAGAACAAGA+AGG | 0.581850 | 6.1:+63554752 | MS.gene82097:CDS |
| CTAGCTATCGAACGCGCCAT+CGG | 0.587439 | 6.1:+63553741 | MS.gene82097:CDS |
| AAAGTACTAGATGTTGGTTG+TGG | 0.613287 | 6.1:+63553636 | MS.gene82097:CDS |
| GAGAAATCATGTTTATGGAG+AGG | 0.618544 | 6.1:-63553719 | None:intergenic |
| TGGAAGGCTAAGCAGATGAG+AGG | 0.635467 | 6.1:+63555307 | MS.gene82097:CDS |
| GCAAAGTTAGGACTAAAGCC+GGG | 0.639688 | 6.1:+63553609 | MS.gene82097:CDS |
| TTCTTGAGCAGAAACAACAA+GGG | 0.645125 | 6.1:+63553578 | MS.gene82097:intron |
| TCCCACAAGAGAAAGCTCAA+AGG | 0.650134 | 6.1:-63552934 | None:intergenic |
| TACAAGTGGTTGAAGCCTGG+AGG | 0.656549 | 6.1:+63553997 | MS.gene82097:CDS |
| GTAGCTGGTGAGCAGATATG+GGG | 0.669642 | 6.1:+63555331 | MS.gene82097:CDS |
| TTTCATTGATGATTTCTCTG+AGG | 0.680525 | 6.1:+63554779 | MS.gene82097:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 63542111 | 63555378 | 63542111 | ID=MS.gene82097 |
| chr6.1 | mRNA | 63542111 | 63555378 | 63542111 | ID=MS.gene82097.t1;Parent=MS.gene82097 |
| chr6.1 | exon | 63542111 | 63542260 | 63542111 | ID=MS.gene82097.t1.exon1;Parent=MS.gene82097.t1 |
| chr6.1 | CDS | 63542111 | 63542260 | 63542111 | ID=cds.MS.gene82097.t1;Parent=MS.gene82097.t1 |
| chr6.1 | exon | 63551509 | 63551646 | 63551509 | ID=MS.gene82097.t1.exon2;Parent=MS.gene82097.t1 |
| chr6.1 | CDS | 63551509 | 63551646 | 63551509 | ID=cds.MS.gene82097.t1;Parent=MS.gene82097.t1 |
| chr6.1 | exon | 63551942 | 63552079 | 63551942 | ID=MS.gene82097.t1.exon3;Parent=MS.gene82097.t1 |
| chr6.1 | CDS | 63551942 | 63552079 | 63551942 | ID=cds.MS.gene82097.t1;Parent=MS.gene82097.t1 |
| chr6.1 | exon | 63552333 | 63552476 | 63552333 | ID=MS.gene82097.t1.exon4;Parent=MS.gene82097.t1 |
| chr6.1 | CDS | 63552333 | 63552476 | 63552333 | ID=cds.MS.gene82097.t1;Parent=MS.gene82097.t1 |
| chr6.1 | exon | 63552894 | 63553004 | 63552894 | ID=MS.gene82097.t1.exon5;Parent=MS.gene82097.t1 |
| chr6.1 | CDS | 63552894 | 63553004 | 63552894 | ID=cds.MS.gene82097.t1;Parent=MS.gene82097.t1 |
| chr6.1 | exon | 63553117 | 63553258 | 63553117 | ID=MS.gene82097.t1.exon6;Parent=MS.gene82097.t1 |
| chr6.1 | CDS | 63553117 | 63553258 | 63553117 | ID=cds.MS.gene82097.t1;Parent=MS.gene82097.t1 |
| chr6.1 | exon | 63553589 | 63553869 | 63553589 | ID=MS.gene82097.t1.exon7;Parent=MS.gene82097.t1 |
| chr6.1 | CDS | 63553589 | 63553869 | 63553589 | ID=cds.MS.gene82097.t1;Parent=MS.gene82097.t1 |
| chr6.1 | exon | 63553970 | 63554131 | 63553970 | ID=MS.gene82097.t1.exon8;Parent=MS.gene82097.t1 |
| chr6.1 | CDS | 63553970 | 63554131 | 63553970 | ID=cds.MS.gene82097.t1;Parent=MS.gene82097.t1 |
| chr6.1 | exon | 63554586 | 63554639 | 63554586 | ID=MS.gene82097.t1.exon9;Parent=MS.gene82097.t1 |
| chr6.1 | CDS | 63554586 | 63554639 | 63554586 | ID=cds.MS.gene82097.t1;Parent=MS.gene82097.t1 |
| chr6.1 | exon | 63554726 | 63554800 | 63554726 | ID=MS.gene82097.t1.exon10;Parent=MS.gene82097.t1 |
| chr6.1 | CDS | 63554726 | 63554800 | 63554726 | ID=cds.MS.gene82097.t1;Parent=MS.gene82097.t1 |
| chr6.1 | exon | 63555280 | 63555378 | 63555280 | ID=MS.gene82097.t1.exon11;Parent=MS.gene82097.t1 |
| chr6.1 | CDS | 63555280 | 63555378 | 63555280 | ID=cds.MS.gene82097.t1;Parent=MS.gene82097.t1 |
| Gene Sequence |
| Protein sequence |
