Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82416.t1 | XP_003607854.1 | 96.6 | 413 | 13 | 1 | 1 | 412 | 13 | 425 | 2.70E-229 | 804.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82416.t1 | Q9MA87 | 62.8 | 427 | 137 | 9 | 8 | 412 | 16 | 442 | 9.2e-146 | 518.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82416.t1 | G7JIR1 | 96.6 | 413 | 13 | 1 | 1 | 412 | 13 | 425 | 1.9e-229 | 804.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052674 | MS.gene82416 | 0.823823 | 1.13E-53 | -1.69E-46 |
| MS.gene052675 | MS.gene82416 | 0.822348 | 2.49E-53 | -1.69E-46 |
| MS.gene052677 | MS.gene82416 | 0.823206 | 1.57E-53 | -1.69E-46 |
| MS.gene057208 | MS.gene82416 | 0.803608 | 3.21E-49 | -1.69E-46 |
| MS.gene060850 | MS.gene82416 | 0.81183 | 5.75E-51 | -1.69E-46 |
| MS.gene060851 | MS.gene82416 | 0.82751 | 1.50E-54 | -1.69E-46 |
| MS.gene060852 | MS.gene82416 | 0.824088 | 9.75E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82416.t1 | MTR_4g083590 | 96.610 | 413 | 13 | 1 | 1 | 412 | 13 | 425 | 0.0 | 825 |
| MS.gene82416.t1 | MTR_2g028900 | 70.824 | 425 | 106 | 5 | 3 | 410 | 9 | 432 | 0.0 | 608 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene82416.t1 | AT3G05320 | 62.763 | 427 | 137 | 9 | 8 | 412 | 16 | 442 | 0.0 | 538 |
| MS.gene82416.t1 | AT3G05320 | 62.763 | 427 | 137 | 9 | 8 | 412 | 16 | 442 | 0.0 | 538 |
| MS.gene82416.t1 | AT3G05320 | 62.763 | 427 | 137 | 9 | 8 | 412 | 16 | 442 | 0.0 | 538 |
Find 61 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTCAAAATTCTTCAATAT+TGG | 0.230483 | 4.2:-26054385 | MS.gene82416:CDS |
| AACACAAAAGATGATCAAAT+TGG | 0.268660 | 4.2:-26054187 | MS.gene82416:CDS |
| AGCAAGAATGTTTCATCATA+TGG | 0.290833 | 4.2:-26055146 | MS.gene82416:CDS |
| GTGGACAGTTCAAAGGGATT+TGG | 0.314132 | 4.2:-26054817 | MS.gene82416:CDS |
| CTTTCTTGTGGCATGATCAT+TGG | 0.319430 | 4.2:-26054725 | MS.gene82416:CDS |
| ACCTTTGCATAGTCCTTAAC+AGG | 0.326962 | 4.2:+26054702 | None:intergenic |
| CCCTTGCAAATGCAATCTTT+TGG | 0.340133 | 4.2:+26055063 | None:intergenic |
| ATTGCAGACTGAATTAAATA+AGG | 0.349906 | 4.2:+26054291 | None:intergenic |
| CCAAGTTTCTTCTTCTCATA+AGG | 0.367277 | 4.2:+26054339 | None:intergenic |
| GTTGGTAACCATGATGATAA+AGG | 0.386254 | 4.2:-26054609 | MS.gene82416:CDS |
| GCAAACTAATGAATTTAGAT+TGG | 0.392348 | 4.2:+26055241 | None:intergenic |
| ATGAATTACCTTTATCATCA+TGG | 0.396445 | 4.2:+26054601 | None:intergenic |
| ATTCTTCAATATTGGAAGGT+TGG | 0.400853 | 4.2:-26054377 | MS.gene82416:CDS |
| ACATGAGAGTAGAAATTGAT+TGG | 0.407692 | 4.2:-26054548 | MS.gene82416:CDS |
| TAGTAGCAAGAATGAGATAA+TGG | 0.408374 | 4.2:-26054475 | MS.gene82416:CDS |
| ACTGCAAGATAAAGAACAAC+TGG | 0.413545 | 4.2:+26054420 | None:intergenic |
| TTTAATCCCCACACAATTTG+AGG | 0.419362 | 4.2:+26055089 | None:intergenic |
| TTGAGAGCTGATATCTTTGT+TGG | 0.421531 | 4.2:-26054252 | MS.gene82416:CDS |
| AACACCTTGTCAAATGAAAT+CGG | 0.421780 | 4.2:+26054951 | None:intergenic |
| CATTCTTGCTCATAAATACA+AGG | 0.428235 | 4.2:+26055159 | None:intergenic |
| ATTGAGGTGATTAAGATTGA+TGG | 0.430449 | 4.2:-26054756 | MS.gene82416:CDS |
| TTTATATTATATGCAAAAGA+AGG | 0.438653 | 4.2:+26054129 | None:intergenic |
| GCCTCTATGATTAGAAAAGT+TGG | 0.443332 | 4.2:-26054627 | MS.gene82416:CDS |
| GCATGATCATTGGCCTGTTA+AGG | 0.447996 | 4.2:-26054715 | MS.gene82416:CDS |
| ATATTGGAAGGTTGGAAAGA+AGG | 0.448216 | 4.2:-26054369 | MS.gene82416:CDS |
| ATGAGATAATGGAAAGAGTA+GGG | 0.459739 | 4.2:-26054464 | MS.gene82416:CDS |
| ACACAAAAGATGATCAAATT+GGG | 0.466064 | 4.2:-26054186 | MS.gene82416:CDS |
| GGAGGTTCCTCAAATTGTGT+GGG | 0.483333 | 4.2:-26055096 | MS.gene82416:CDS |
| CAATGATCATGCCACAAGAA+AGG | 0.489335 | 4.2:+26054726 | None:intergenic |
| CCTTATGAGAAGAAGAAACT+TGG | 0.492913 | 4.2:-26054339 | MS.gene82416:CDS |
| AATGAGATAATGGAAAGAGT+AGG | 0.495512 | 4.2:-26054465 | MS.gene82416:CDS |
| TGGAGGTTCCTCAAATTGTG+TGG | 0.500076 | 4.2:-26055097 | MS.gene82416:CDS |
| ACCAAAAGATTGCATTTGCA+AGG | 0.505247 | 4.2:-26055064 | MS.gene82416:CDS |
| TTGAGGTGATTAAGATTGAT+GGG | 0.506436 | 4.2:-26054755 | MS.gene82416:CDS |
| AATGATCATGCCACAAGAAA+GGG | 0.506447 | 4.2:+26054727 | None:intergenic |
| CAAAATTCTTCAATATTGGA+AGG | 0.510361 | 4.2:-26054381 | MS.gene82416:CDS |
| AATTGAATGGTCCTATGAGA+TGG | 0.511997 | 4.2:-26054101 | MS.gene82416:CDS |
| AATATAAAAGGAGAATTGAA+TGG | 0.513612 | 4.2:-26054114 | MS.gene82416:CDS |
| ATATTTGTAACCCATCTCAT+AGG | 0.515941 | 4.2:+26054090 | None:intergenic |
| CTCATGTGAACAGCAACATA+AGG | 0.518574 | 4.2:+26054564 | None:intergenic |
| GTTGAAATGCAGAAAGGAAG+TGG | 0.524410 | 4.2:-26054846 | MS.gene82416:CDS |
| CCAAAAGATTGCATTTGCAA+GGG | 0.530122 | 4.2:-26055063 | MS.gene82416:CDS |
| AGCCTTCATGCTATCAGCTA+TGG | 0.542544 | 4.2:-26054057 | MS.gene82416:CDS |
| ATCAATTTCCTTGTAAAACA+AGG | 0.547846 | 4.2:+26054983 | None:intergenic |
| GTAGAAAGTGGACAGTTCAA+AGG | 0.550514 | 4.2:-26054824 | MS.gene82416:CDS |
| GCCTGTTAAGGACTATGCAA+AGG | 0.559420 | 4.2:-26054703 | MS.gene82416:CDS |
| GAGGTTCCTCAAATTGTGTG+GGG | 0.573751 | 4.2:-26055095 | MS.gene82416:CDS |
| ATTGAATGGTCCTATGAGAT+GGG | 0.575314 | 4.2:-26054100 | MS.gene82416:CDS |
| TAGAAAGTGGACAGTTCAAA+GGG | 0.575946 | 4.2:-26054823 | MS.gene82416:CDS |
| ATAGCTTTCAACAAGAAATG+TGG | 0.601889 | 4.2:+26054028 | None:intergenic |
| CTTGAGTATATTCATCAACC+AGG | 0.612021 | 4.2:+26054664 | None:intergenic |
| GAAGTGGTTGAAATGCAGAA+AGG | 0.612730 | 4.2:-26054852 | MS.gene82416:CDS |
| AAATTTAATGAACTGTGTAG+TGG | 0.613501 | 4.2:-26054918 | MS.gene82416:CDS |
| TCTCTTGAACATAACAGAAG+TGG | 0.614096 | 4.2:-26054868 | MS.gene82416:CDS |
| AGAAAGGAAGTGGTAGAAAG+TGG | 0.623667 | 4.2:-26054836 | MS.gene82416:CDS |
| TGCCATAGCTGATAGCATGA+AGG | 0.627885 | 4.2:+26054055 | None:intergenic |
| TGTGTGGAAGTGATGTAAGA+TGG | 0.635629 | 4.2:-26054152 | MS.gene82416:CDS |
| AACAAGGAAGCACTAAGACT+AGG | 0.654550 | 4.2:+26054999 | None:intergenic |
| ACAACCGATTTCATTTGACA+AGG | 0.662499 | 4.2:-26054955 | MS.gene82416:CDS |
| TTGGGTGTGAATGAAATGTG+TGG | 0.683523 | 4.2:-26054168 | MS.gene82416:CDS |
| AAGCTGATAAAGTTAATGGG+TGG | 0.773450 | 4.2:-26055266 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTATATTATATGCAAAAGA+AGG | + | chr4.2:26055160-26055179 | None:intergenic | 15.0% |
| !!! | TCTTTTGCATATAATATAAA+AGG | - | chr4.2:26055160-26055179 | MS.gene82416:CDS | 15.0% |
| !! | AATATAAAAGGAGAATTGAA+TGG | - | chr4.2:26055172-26055191 | MS.gene82416:CDS | 20.0% |
| !! | ACTTCAAAATTCTTCAATAT+TGG | - | chr4.2:26054901-26054920 | MS.gene82416:CDS | 20.0% |
| ! | AAATTTAATGAACTGTGTAG+TGG | - | chr4.2:26054368-26054387 | MS.gene82416:CDS | 25.0% |
| ! | AACACAAAAGATGATCAAAT+TGG | - | chr4.2:26055099-26055118 | MS.gene82416:CDS | 25.0% |
| ! | ACACAAAAGATGATCAAATT+GGG | - | chr4.2:26055100-26055119 | MS.gene82416:CDS | 25.0% |
| ! | ATCAATTTCCTTGTAAAACA+AGG | + | chr4.2:26054306-26054325 | None:intergenic | 25.0% |
| ! | ATGAATTACCTTTATCATCA+TGG | + | chr4.2:26054688-26054707 | None:intergenic | 25.0% |
| ! | ATTGCAGACTGAATTAAATA+AGG | + | chr4.2:26054998-26055017 | None:intergenic | 25.0% |
| ! | CAAAATTCTTCAATATTGGA+AGG | - | chr4.2:26054905-26054924 | MS.gene82416:CDS | 25.0% |
| ! | CAGTTGAAACAGTATAAAAA+TGG | - | chr4.2:26054494-26054513 | MS.gene82416:CDS | 25.0% |
| ! | GATGATTCATTGCAAAAAAT+TGG | - | chr4.2:26054760-26054779 | MS.gene82416:CDS | 25.0% |
| ! | GCAAACTAATGAATTTAGAT+TGG | + | chr4.2:26054048-26054067 | None:intergenic | 25.0% |
| !! | TGTTCAACAACAAAAGTTTT+TGG | - | chr4.2:26054169-26054188 | MS.gene82416:CDS | 25.0% |
| !!! | TATTGCTGTTTTGATTCTAA+GGG | - | chr4.2:26054085-26054104 | MS.gene82416:CDS | 25.0% |
| !!! | TTATTGCTGTTTTGATTCTA+AGG | - | chr4.2:26054084-26054103 | MS.gene82416:CDS | 25.0% |
| AACACCTTGTCAAATGAAAT+CGG | + | chr4.2:26054338-26054357 | None:intergenic | 30.0% | |
| ACATGAGAGTAGAAATTGAT+TGG | - | chr4.2:26054738-26054757 | MS.gene82416:CDS | 30.0% | |
| AGCAAGAATGTTTCATCATA+TGG | - | chr4.2:26054140-26054159 | MS.gene82416:CDS | 30.0% | |
| ATATTTGTAACCCATCTCAT+AGG | + | chr4.2:26055199-26055218 | None:intergenic | 30.0% | |
| ATTCTTCAATATTGGAAGGT+TGG | - | chr4.2:26054909-26054928 | MS.gene82416:CDS | 30.0% | |
| ATTGAGGTGATTAAGATTGA+TGG | - | chr4.2:26054530-26054549 | MS.gene82416:CDS | 30.0% | |
| CATTCTTGCTCATAAATACA+AGG | + | chr4.2:26054130-26054149 | None:intergenic | 30.0% | |
| TAGTAGCAAGAATGAGATAA+TGG | - | chr4.2:26054811-26054830 | MS.gene82416:CDS | 30.0% | |
| TTGAGGTGATTAAGATTGAT+GGG | - | chr4.2:26054531-26054550 | MS.gene82416:CDS | 30.0% | |
| ! | ACCAACTTTTCTAATCATAG+AGG | + | chr4.2:26054663-26054682 | None:intergenic | 30.0% |
| ! | CTCATAAATACAAGGTTTTG+AGG | + | chr4.2:26054122-26054141 | None:intergenic | 30.0% |
| !! | AATGAGATAATGGAAAGAGT+AGG | - | chr4.2:26054821-26054840 | MS.gene82416:CDS | 30.0% |
| !! | ATGAGATAATGGAAAGAGTA+GGG | - | chr4.2:26054822-26054841 | MS.gene82416:CDS | 30.0% |
| !! | TCAACAACAAAAGTTTTTGG+AGG | - | chr4.2:26054172-26054191 | MS.gene82416:CDS | 30.0% |
| !! | TGGTTCTTTTGATGATATTG+AGG | - | chr4.2:26054514-26054533 | MS.gene82416:CDS | 30.0% |
| !!! | TGTAGTGGTTTTGTTCAATT+GGG | - | chr4.2:26054383-26054402 | MS.gene82416:CDS | 30.0% |
| AATGATCATGCCACAAGAAA+GGG | + | chr4.2:26054562-26054581 | None:intergenic | 35.0% | |
| AATTGAATGGTCCTATGAGA+TGG | - | chr4.2:26055185-26055204 | MS.gene82416:CDS | 35.0% | |
| ACCAAAAGATTGCATTTGCA+AGG | - | chr4.2:26054222-26054241 | MS.gene82416:CDS | 35.0% | |
| ACTGCAAGATAAAGAACAAC+TGG | + | chr4.2:26054869-26054888 | None:intergenic | 35.0% | |
| ATATTGGAAGGTTGGAAAGA+AGG | - | chr4.2:26054917-26054936 | MS.gene82416:CDS | 35.0% | |
| ATTGAATGGTCCTATGAGAT+GGG | - | chr4.2:26055186-26055205 | MS.gene82416:CDS | 35.0% | |
| CCAAAAGATTGCATTTGCAA+GGG | - | chr4.2:26054223-26054242 | MS.gene82416:CDS | 35.0% | |
| CCAAGTTTCTTCTTCTCATA+AGG | + | chr4.2:26054950-26054969 | None:intergenic | 35.0% | |
| CCTTATGAGAAGAAGAAACT+TGG | - | chr4.2:26054947-26054966 | MS.gene82416:CDS | 35.0% | |
| CTTGAGTATATTCATCAACC+AGG | + | chr4.2:26054625-26054644 | None:intergenic | 35.0% | |
| GCCTCTATGATTAGAAAAGT+TGG | - | chr4.2:26054659-26054678 | MS.gene82416:CDS | 35.0% | |
| TAGAAAGTGGACAGTTCAAA+GGG | - | chr4.2:26054463-26054482 | MS.gene82416:CDS | 35.0% | |
| TCTCTTGAACATAACAGAAG+TGG | - | chr4.2:26054418-26054437 | MS.gene82416:CDS | 35.0% | |
| TTGAGAGCTGATATCTTTGT+TGG | - | chr4.2:26055034-26055053 | MS.gene82416:CDS | 35.0% | |
| TTTAATCCCCACACAATTTG+AGG | + | chr4.2:26054200-26054219 | None:intergenic | 35.0% | |
| ! | ACAACCGATTTCATTTGACA+AGG | - | chr4.2:26054331-26054350 | MS.gene82416:CDS | 35.0% |
| !! | GTTGGTAACCATGATGATAA+AGG | - | chr4.2:26054677-26054696 | MS.gene82416:CDS | 35.0% |
| !!! | GTGTAGTGGTTTTGTTCAAT+TGG | - | chr4.2:26054382-26054401 | MS.gene82416:CDS | 35.0% |
| !!! | TAGTGCTTCCTTGTTTTACA+AGG | - | chr4.2:26054295-26054314 | MS.gene82416:CDS | 35.0% |
| ACCTTTGCATAGTCCTTAAC+AGG | + | chr4.2:26054587-26054606 | None:intergenic | 40.0% | |
| AGAAAGGAAGTGGTAGAAAG+TGG | - | chr4.2:26054450-26054469 | MS.gene82416:CDS | 40.0% | |
| ATGGGAAAAACCCTTTCTTG+TGG | - | chr4.2:26054549-26054568 | MS.gene82416:CDS | 40.0% | |
| CAATGATCATGCCACAAGAA+AGG | + | chr4.2:26054563-26054582 | None:intergenic | 40.0% | |
| CTCATGTGAACAGCAACATA+AGG | + | chr4.2:26054725-26054744 | None:intergenic | 40.0% | |
| CTTTCTTGTGGCATGATCAT+TGG | - | chr4.2:26054561-26054580 | MS.gene82416:CDS | 40.0% | |
| GAAGTGGTTGAAATGCAGAA+AGG | - | chr4.2:26054434-26054453 | MS.gene82416:CDS | 40.0% | |
| GTAGAAAGTGGACAGTTCAA+AGG | - | chr4.2:26054462-26054481 | MS.gene82416:CDS | 40.0% | |
| GTTGAAATGCAGAAAGGAAG+TGG | - | chr4.2:26054440-26054459 | MS.gene82416:CDS | 40.0% | |
| TGTGTGGAAGTGATGTAAGA+TGG | - | chr4.2:26055134-26055153 | MS.gene82416:CDS | 40.0% | |
| TTGGGTGTGAATGAAATGTG+TGG | - | chr4.2:26055118-26055137 | MS.gene82416:CDS | 40.0% | |
| ! | CCCTTGCAAATGCAATCTTT+TGG | + | chr4.2:26054226-26054245 | None:intergenic | 40.0% |
| !! | AACAAGGAAGCACTAAGACT+AGG | + | chr4.2:26054290-26054309 | None:intergenic | 40.0% |
| AGCCTTCATGCTATCAGCTA+TGG | - | chr4.2:26055229-26055248 | MS.gene82416:CDS | 45.0% | |
| GAGGTTCCTCAAATTGTGTG+GGG | - | chr4.2:26054191-26054210 | MS.gene82416:CDS | 45.0% | |
| GCCTGTTAAGGACTATGCAA+AGG | - | chr4.2:26054583-26054602 | MS.gene82416:CDS | 45.0% | |
| GGAGGTTCCTCAAATTGTGT+GGG | - | chr4.2:26054190-26054209 | MS.gene82416:CDS | 45.0% | |
| GTGGACAGTTCAAAGGGATT+TGG | - | chr4.2:26054469-26054488 | MS.gene82416:CDS | 45.0% | |
| TGCCATAGCTGATAGCATGA+AGG | + | chr4.2:26055234-26055253 | None:intergenic | 45.0% | |
| TGGAGGTTCCTCAAATTGTG+TGG | - | chr4.2:26054189-26054208 | MS.gene82416:CDS | 45.0% | |
| ! | GCATGATCATTGGCCTGTTA+AGG | - | chr4.2:26054571-26054590 | MS.gene82416:CDS | 45.0% |
| !!! | GGTTTTTGAGTGCTTAGTCC+TGG | - | chr4.2:26054604-26054623 | MS.gene82416:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 26054035 | 26055273 | 26054035 | ID=MS.gene82416 |
| chr4.2 | mRNA | 26054035 | 26055273 | 26054035 | ID=MS.gene82416.t1;Parent=MS.gene82416 |
| chr4.2 | exon | 26054035 | 26055273 | 26054035 | ID=MS.gene82416.t1.exon1;Parent=MS.gene82416.t1 |
| chr4.2 | CDS | 26054035 | 26055273 | 26054035 | ID=cds.MS.gene82416.t1;Parent=MS.gene82416.t1 |
| Gene Sequence |
| Protein sequence |
