AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene82964

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82964.t1 MTR_8g071280 97.483 596 15 0 1 596 1 596 0.0 1211
MS.gene82964.t1 MTR_5g026930 76.333 600 136 3 1 596 1 598 0.0 961
MS.gene82964.t1 MTR_5g026930 76.447 501 112 3 1 497 1 499 0.0 801
MS.gene82964.t1 MTR_1g101370 49.262 542 262 9 57 588 79 617 0.0 550
MS.gene82964.t1 MTR_8g432680 49.451 546 258 10 52 588 94 630 0.0 541
MS.gene82964.t1 MTR_0019s0120 48.921 556 266 10 43 590 83 628 0.0 533
MS.gene82964.t1 MTR_7g076170 50.947 528 245 10 71 588 102 625 0.0 529
MS.gene82964.t1 MTR_8g100075 45.636 550 283 10 58 595 82 627 9.61e-175 509
MS.gene82964.t1 MTR_8g055840 42.314 605 324 10 10 595 9 607 2.41e-168 493
MS.gene82964.t1 MTR_5g040360 45.109 552 290 8 45 590 57 601 5.07e-167 489
MS.gene82964.t1 MTR_7g006060 45.554 551 282 10 45 582 20 565 6.66e-167 488
MS.gene82964.t1 MTR_4g098890 47.637 529 262 8 66 587 82 602 4.03e-166 514
MS.gene82964.t1 MTR_3g040370 43.023 602 308 12 16 595 16 604 3.33e-165 484
MS.gene82964.t1 MTR_8g432620 41.423 548 293 8 52 588 70 600 2.49e-156 461
MS.gene82964.t1 MTR_7g076150 41.219 558 297 9 45 588 47 587 3.31e-155 458
MS.gene82964.t1 MTR_5g080720 42.831 544 286 11 53 585 108 637 1.37e-152 453
MS.gene82964.t1 MTR_0019s0120 49.351 462 217 9 43 497 83 534 4.44e-151 446
MS.gene82964.t1 MTR_3g068990 42.251 542 286 11 57 587 142 667 8.59e-146 437
MS.gene82964.t1 MTR_1g442840 37.460 622 333 10 11 591 12 618 5.80e-145 433
MS.gene82964.t1 MTR_1g075900 38.782 624 324 11 11 592 11 618 1.39e-144 432
MS.gene82964.t1 MTR_1g029730 42.748 524 269 13 79 587 151 658 9.13e-144 431
MS.gene82964.t1 MTR_1g069785 38.833 600 326 12 11 591 11 588 5.27e-143 427
MS.gene82964.t1 MTR_1g442840 41.833 502 271 6 101 591 14 505 5.34e-143 424
MS.gene82964.t1 MTR_3g096480 42.424 528 267 12 79 588 268 776 2.96e-137 419
MS.gene82964.t1 MTR_5g021100 41.333 525 273 13 79 588 329 833 3.13e-137 420
MS.gene82964.t1 MTR_8g076650 40.146 548 301 11 52 588 359 890 2.90e-136 419
MS.gene82964.t1 MTR_8g093880 40.000 565 299 14 44 588 262 806 1.63e-133 410
MS.gene82964.t1 MTR_5g080720 43.035 481 250 10 53 523 108 574 1.80e-132 399
MS.gene82964.t1 MTR_7g076150 43.384 461 233 8 139 588 1 444 4.99e-132 394
MS.gene82964.t1 MTR_5g083150 38.846 520 286 9 79 588 101 598 1.19e-129 393
MS.gene82964.t1 MTR_3g088790 40.000 525 285 11 79 588 233 742 2.83e-126 389
MS.gene82964.t1 MTR_3g071530 39.808 520 277 11 79 588 153 646 5.96e-126 385
MS.gene82964.t1 MTR_1g051055 36.975 595 333 12 22 588 23 603 4.66e-124 379
MS.gene82964.t1 MTR_1g022255 36.973 522 288 13 78 588 208 699 5.00e-107 337
MS.gene82964.t1 MTR_4g083030 33.213 554 316 13 59 585 75 601 6.28e-94 300
MS.gene82964.t1 MTR_3g074780 30.755 556 337 15 64 588 150 688 3.50e-92 298
MS.gene82964.t1 MTR_5g064080 31.492 543 318 15 73 585 149 667 6.97e-92 297
MS.gene82964.t1 MTR_1g022255 33.504 391 223 11 78 458 208 571 2.20e-60 211
MS.gene82964.t1 MTR_5g033760 45.455 66 36 0 531 596 41 106 2.90e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene82964.t1 AT4G19120 73.277 595 148 3 7 596 10 598 0.0 942
MS.gene82964.t1 AT4G19120 73.277 595 148 3 7 596 10 598 0.0 942
MS.gene82964.t1 AT1G31850 71.864 590 159 3 12 595 15 603 0.0 929
MS.gene82964.t1 AT1G31850 71.864 590 159 3 12 595 15 603 0.0 929
MS.gene82964.t1 AT1G31850 71.864 590 159 3 12 595 15 603 0.0 929
MS.gene82964.t1 AT1G31850 71.864 590 159 3 12 595 15 603 0.0 929
MS.gene82964.t1 AT4G19120 73.790 496 119 3 7 497 10 499 0.0 785
MS.gene82964.t1 AT4G10440 47.004 534 270 9 70 594 93 622 7.50e-180 523
MS.gene82964.t1 AT4G00750 44.079 608 307 11 18 595 25 629 1.02e-179 523
MS.gene82964.t1 AT1G33170 47.259 529 265 10 70 588 110 634 1.96e-176 514
MS.gene82964.t1 AT2G45750 47.290 535 266 9 67 588 84 615 5.74e-176 513
MS.gene82964.t1 AT4G18030 42.414 613 307 10 10 592 11 607 6.18e-171 499
MS.gene82964.t1 AT1G26850 45.321 545 282 9 61 595 77 615 1.11e-165 486
MS.gene82964.t1 AT1G26850 45.321 545 282 9 61 595 77 615 1.11e-165 486
MS.gene82964.t1 AT4G00740 44.275 524 270 7 73 588 84 593 1.06e-162 478
MS.gene82964.t1 AT4G00740 44.275 524 270 7 73 588 84 593 1.06e-162 478
MS.gene82964.t1 AT2G43200 45.908 501 262 8 70 565 91 587 2.60e-155 459
MS.gene82964.t1 AT2G43200 45.908 501 262 8 70 565 91 587 2.60e-155 459
MS.gene82964.t1 AT2G39750 43.831 543 280 11 56 587 163 691 1.23e-152 455
MS.gene82964.t1 AT1G77260 40.455 571 309 14 28 587 105 655 2.22e-144 433
MS.gene82964.t1 AT3G23300 38.972 603 331 8 16 591 12 604 4.99e-143 428
MS.gene82964.t1 AT3G23300 38.972 603 331 8 16 591 12 604 4.99e-143 428
MS.gene82964.t1 AT1G04430 37.778 630 338 11 1 591 2 616 6.94e-142 425
MS.gene82964.t1 AT1G04430 37.778 630 338 11 1 591 2 616 6.94e-142 425
MS.gene82964.t1 AT1G04430 37.778 630 338 11 1 591 2 616 6.94e-142 425
MS.gene82964.t1 AT5G06050 40.556 540 292 11 61 587 140 663 1.70e-141 426
MS.gene82964.t1 AT4G14360 41.155 537 292 7 69 591 75 601 8.98e-140 419
MS.gene82964.t1 AT4G14360 41.155 537 292 7 69 591 75 601 8.98e-140 419
MS.gene82964.t1 AT2G40280 39.763 591 306 14 21 596 34 589 1.60e-138 416
MS.gene82964.t1 AT3G56080 43.750 496 251 14 79 564 114 591 8.07e-137 412
MS.gene82964.t1 AT2G34300 41.561 551 284 14 56 588 227 757 1.38e-136 416
MS.gene82964.t1 AT2G34300 41.561 551 284 14 56 588 227 757 1.38e-136 416
MS.gene82964.t1 AT2G34300 41.561 551 284 14 56 588 227 757 1.38e-136 416
MS.gene82964.t1 AT1G29470 40.653 551 290 13 56 588 226 757 9.89e-135 412
MS.gene82964.t1 AT1G29470 40.653 551 290 13 56 588 226 757 9.89e-135 412
MS.gene82964.t1 AT5G64030 41.321 530 276 13 79 591 308 819 5.24e-133 409
MS.gene82964.t1 AT5G14430 38.833 600 325 11 17 588 19 604 4.55e-132 400
MS.gene82964.t1 AT5G14430 38.667 600 326 11 17 588 19 604 1.12e-130 396
MS.gene82964.t1 AT3G51070 40.684 526 279 11 79 588 381 889 4.55e-129 400
MS.gene82964.t1 AT3G10200 35.655 603 351 10 6 588 3 588 4.18e-128 389
MS.gene82964.t1 AT1G26850 44.601 426 222 7 61 478 77 496 5.25e-125 378
MS.gene82964.t1 AT3G56080 43.956 455 227 14 79 523 114 550 6.92e-123 374
MS.gene82964.t1 AT5G04060 35.893 599 339 13 17 588 17 597 1.93e-121 372
MS.gene82964.t1 AT3G10200 36.413 552 316 9 6 539 3 537 1.62e-116 359
MS.gene82964.t1 AT3G51070 42.325 456 233 10 79 518 381 822 8.54e-116 364
MS.gene82964.t1 AT1G19430 37.143 525 284 15 78 588 230 722 4.60e-108 341
MS.gene82964.t1 AT1G19430 37.143 525 284 15 78 588 230 722 4.60e-108 341
MS.gene82964.t1 AT1G13860 32.364 550 310 15 70 587 81 600 2.15e-91 294
MS.gene82964.t1 AT1G13860 32.364 550 310 15 70 587 81 600 2.15e-91 294
MS.gene82964.t1 AT1G13860 32.364 550 310 15 70 587 81 600 2.15e-91 294
MS.gene82964.t1 AT1G13860 32.364 550 310 15 70 587 81 600 2.15e-91 294
MS.gene82964.t1 AT1G78240 30.642 545 329 13 73 585 149 676 2.24e-88 288
MS.gene82964.t1 AT1G78240 30.642 545 329 13 73 585 149 676 2.24e-88 288
MS.gene82964.t1 AT2G03480 30.851 564 331 13 55 585 64 601 2.35e-86 281
MS.gene82964.t1 AT2G03480 31.150 565 317 14 55 585 64 590 5.79e-86 279
MS.gene82964.t1 AT1G13860 33.477 463 259 14 154 587 2 444 1.62e-78 255
MS.gene82964.t1 AT1G19430 34.684 395 216 13 78 459 230 595 1.08e-63 220
MS.gene82964.t1 AT2G03480 28.152 341 222 6 55 380 64 396 1.80e-42 158

Find 155 sgRNAs with CRISPR-Local

Find 231 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGTTCCATCTTTCATTTC+TGG 0.146770 8.1:-33561314 None:intergenic
TTCTTCGTCCTGGAGGATTT+TGG 0.151747 8.1:+33562117 MS.gene82964:CDS
TTCTATATGGGTGTAATCTT+TGG 0.198314 8.1:+33560419 MS.gene82964:CDS
CTGCTCTTTGGCCATCTAAT+TGG 0.211320 8.1:-33561027 None:intergenic
ATGTGGACTTTCTTTCTATA+TGG 0.220950 8.1:+33560406 MS.gene82964:CDS
CCCATCAAATTCTTTCGATA+TGG 0.245961 8.1:+33561892 MS.gene82964:CDS
TAGCTGGGGTGGTGATTTAC+TGG 0.259336 8.1:+33561748 MS.gene82964:CDS
CGGTAAATATGTTGATTTAA+TGG 0.261309 8.1:+33561280 MS.gene82964:CDS
CAATCAACATTGGTTGAAAA+AGG 0.293240 8.1:+33561205 MS.gene82964:CDS
TCAAACGTGACGGCAGTAAT+TGG 0.299230 8.1:+33562528 MS.gene82964:CDS
ACTTATTGATGATGATTTCT+TGG 0.300333 8.1:-33560495 None:intergenic
ATGGATATGAATACGGAATA+TGG 0.323237 8.1:+33562617 MS.gene82964:CDS
CGAATTCTGTCCATGGAATA+AGG 0.325163 8.1:-33561931 None:intergenic
GCTAGGCTGTCATCGCATTT+AGG 0.327506 8.1:-33562347 None:intergenic
ACATATTTACCGACACCGTT+CGG 0.328019 8.1:-33561269 None:intergenic
TGTGGACTTTCTTTCTATAT+GGG 0.328602 8.1:+33560407 MS.gene82964:CDS
ATTCGAACGGCCATTGATAC+TGG 0.329336 8.1:+33561335 MS.gene82964:CDS
CAACATTCATCTCTGCTCTT+TGG 0.332417 8.1:-33561039 None:intergenic
GGAGAGAAATTCCTCTTTCC+TGG 0.334164 8.1:+33561230 MS.gene82964:CDS
CTTATTGATGATGATTTCTT+GGG 0.337942 8.1:-33560494 None:intergenic
CGTGGTATCCCTGCAATTCT+TGG 0.339517 8.1:+33561842 MS.gene82964:CDS
CATATTTACCGACACCGTTC+GGG 0.351894 8.1:-33561268 None:intergenic
TTACAGAGATGGAAGAAGTA+TGG 0.359526 8.1:+33560913 MS.gene82964:intron
TAGTTCCATCTTTCATTTCT+GGG 0.364638 8.1:-33561313 None:intergenic
TATGACCTCCTTCATCTTGC+AGG 0.378268 8.1:+33562890 MS.gene82964:CDS
TGTTTGTTAATCCAGTCATA+TGG 0.383257 8.1:-33561176 None:intergenic
TTATTGTAACAATCATTAAC+AGG 0.384939 8.1:-33562302 None:intergenic
ACGACAAAACAAGTGCGAAT+TGG 0.387458 8.1:-33562392 None:intergenic
AGCTCAGGTTCAATTCGCTT+TGG 0.387480 8.1:+33561817 MS.gene82964:CDS
CTTATTCCATGGACAGAATT+CGG 0.393587 8.1:+33561932 MS.gene82964:CDS
AGCCACCAGGTCGTAAAATT+CGG 0.398973 8.1:-33563268 None:intergenic
GATCCAAAGTTTAAGAAAAT+AGG 0.401896 8.1:+33562419 MS.gene82964:CDS
GCAAGGACACCAAGAATTGC+AGG 0.407630 8.1:-33561851 None:intergenic
TGAGCTTGGTGGTTATCTCT+TGG 0.408024 8.1:-33561800 None:intergenic
TGAAATGAAGTACGTATTGT+TGG 0.409457 8.1:+33563238 MS.gene82964:CDS
CCTTGATTGATGATCCTGTA+TGG 0.411232 8.1:+33562654 MS.gene82964:CDS
ATCTTATCAGTCCCAAGCTC+AGG 0.412393 8.1:-33562584 None:intergenic
GAAGTACGTATTGTTGGAAA+TGG 0.413240 8.1:+33563244 MS.gene82964:CDS
TGATAGTCAATACTGCATTC+AGG 0.414829 8.1:-33560545 None:intergenic
TGATACAATTCTATCGTTCT+CGG 0.415528 8.1:-33560444 None:intergenic
CCCTGTGGCAATCATATTTG+TGG 0.438188 8.1:+33560379 MS.gene82964:CDS
ATCCTATCGGTTCATAATGT+TGG 0.442728 8.1:+33560936 MS.gene82964:CDS
ACGGCCATTGATACTGGTTG+TGG 0.443198 8.1:+33561341 MS.gene82964:CDS
CTCCATGTCACCTCCCTGCT+CGG 0.455405 8.1:-33563378 None:intergenic
ACTGGATCGTGGAATTCTTA+CGG 0.459338 8.1:+33561766 MS.gene82964:CDS
TCAGAAATGTTCCATATGAC+TGG 0.460287 8.1:+33561165 MS.gene82964:intron
ACCCTTAGCAATAGTTGTAA+TGG 0.466423 8.1:-33563327 None:intergenic
TGTCCATGGAATAAGGCATC+TGG 0.467386 8.1:-33561924 None:intergenic
GGTACCAGCTTCCAATTAGA+TGG 0.467802 8.1:+33561016 MS.gene82964:CDS
GGAGTATACCATGCTGAATC+TGG 0.477004 8.1:-33562371 None:intergenic
GTTTATGATCGAGGCCTCAT+TGG 0.482541 8.1:+33562722 MS.gene82964:CDS
AAGAAGTTGATCCCTGAGCT+TGG 0.483280 8.1:+33562572 MS.gene82964:CDS
TCGAAAGAATTTGATGGGAA+TGG 0.484261 8.1:-33561887 None:intergenic
TGCCATTACAACTATTGCTA+AGG 0.485514 8.1:+33563325 MS.gene82964:CDS
AAATGTGATGGATATGAATA+CGG 0.485516 8.1:+33562610 MS.gene82964:CDS
ATCCTCCAGGACGAAGAATC+CGG 0.487693 8.1:-33562112 None:intergenic
GCCATATCGAAAGAATTTGA+TGG 0.494406 8.1:-33561893 None:intergenic
CGAACCATTGAGATTCGATC+TGG 0.496305 8.1:-33562485 None:intergenic
GAAAAGTGGTAGACAAGAGT+AGG 0.497707 8.1:+33560351 MS.gene82964:CDS
GATCTGCGCTCGTAGTTGAT+TGG 0.499029 8.1:-33562155 None:intergenic
GAAGAATAATGAAGGAAAAG+TGG 0.501394 8.1:+33560337 MS.gene82964:CDS
AGAAGTTGATCCCTGAGCTT+GGG 0.508058 8.1:+33562573 MS.gene82964:CDS
AAGAGATAACCACCAAGCTC+AGG 0.511627 8.1:+33561802 MS.gene82964:CDS
AGCTGGTACCCATCCGGAGG+AGG 0.516830 8.1:-33561003 None:intergenic
TCATTGGAACGTTCCACGAC+TGG 0.519029 8.1:+33562738 MS.gene82964:CDS
TGAAGAGTCCTGCAAGATGA+AGG 0.520104 8.1:-33562898 None:intergenic
TTGGCCATCTAATTGGAAGC+TGG 0.520718 8.1:-33561020 None:intergenic
CTGATCCCAGAAATGAAAGA+TGG 0.520799 8.1:+33561308 MS.gene82964:CDS
GGTGTAACATGCAACCGCTC+GGG 0.520828 8.1:-33562464 None:intergenic
ATTGGAAGCTGGTACCCATC+CGG 0.522262 8.1:-33561009 None:intergenic
CGAATTGAACCTGAGCTTGG+TGG 0.522362 8.1:-33561811 None:intergenic
GCTCCAGATGCCTTATTCCA+TGG 0.526600 8.1:+33561921 MS.gene82964:CDS
TGCTTAGTTCCTCCTCCGGA+TGG 0.527581 8.1:+33560994 MS.gene82964:CDS
TGGTGTAACATGCAACCGCT+CGG 0.527597 8.1:-33562465 None:intergenic
AAATTCCTCTTTCCTGGTGG+AGG 0.529642 8.1:+33561236 MS.gene82964:CDS
AGAATGCTTAGTTCCTCCTC+CGG 0.532704 8.1:+33560990 MS.gene82964:CDS
ACCACATTCATAACCCATAC+AGG 0.533600 8.1:-33562668 None:intergenic
CTCGTAGTTGATTGGTGGAC+CGG 0.535746 8.1:-33562147 None:intergenic
TAGTACCTCCACCAGGAAAG+AGG 0.538108 8.1:-33561241 None:intergenic
CACTCCATGCACTGATCCAA+GGG 0.540931 8.1:+33560574 MS.gene82964:CDS
CACCCCACAACCAGTATCAA+TGG 0.542219 8.1:-33561345 None:intergenic
AAGCGAATTGAACCTGAGCT+TGG 0.543694 8.1:-33561814 None:intergenic
CGGCCATTGATACTGGTTGT+GGG 0.544765 8.1:+33561342 MS.gene82964:CDS
GCAGAGATGAATGTTGGTAC+AGG 0.545315 8.1:+33561046 MS.gene82964:CDS
TACCGAGCAGGGAGGTGACA+TGG 0.545935 8.1:+33563376 MS.gene82964:CDS
TGATAAGATAAGAAATGTGA+TGG 0.548109 8.1:+33562598 MS.gene82964:CDS
CGAATCTCAATGGTTCGTCA+TGG 0.550079 8.1:+33562491 MS.gene82964:CDS
GTTCAATTCGCTTTGGAGCG+TGG 0.551979 8.1:+33561824 MS.gene82964:CDS
ATCATATTTGTGGTGCTATG+TGG 0.553513 8.1:+33560389 MS.gene82964:CDS
CTGCGCTCGTAGTTGATTGG+TGG 0.554325 8.1:-33562152 None:intergenic
ACCACAAATATGATTGCCAC+AGG 0.556765 8.1:-33560380 None:intergenic
TTAATATTCTTGAAATACAC+CGG 0.559268 8.1:+33562093 MS.gene82964:CDS
ACACTCCATGCACTGATCCA+AGG 0.560389 8.1:+33560573 MS.gene82964:CDS
ATACACCGGATTCTTCGTCC+TGG 0.561320 8.1:+33562107 MS.gene82964:CDS
AACTACGAGCGCAGATCGCG+TGG 0.563131 8.1:+33562161 MS.gene82964:CDS
TACACCAGATCGAATCTCAA+TGG 0.564334 8.1:+33562481 MS.gene82964:CDS
CCATATCGAAAGAATTTGAT+GGG 0.566568 8.1:-33561892 None:intergenic
GTCTTACATCAATTTCTAAG+TGG 0.567739 8.1:+33562441 MS.gene82964:CDS
ACTATGTTCCCGAACGGTGT+CGG 0.567956 8.1:+33561260 MS.gene82964:CDS
TCTCGAAAATTGGAGGGCAA+TGG 0.568556 8.1:-33560963 None:intergenic
CTCCCTGTGGTTTATGATCG+AGG 0.568992 8.1:+33562713 MS.gene82964:CDS
ACTGCATTCAGGAAAACTGA+TGG 0.569719 8.1:-33560534 None:intergenic
GAATCTCAATGGTTCGTCAT+GGG 0.570523 8.1:+33562492 MS.gene82964:CDS
CGCACGAGAGCATAGCCACC+AGG 0.572650 8.1:-33563281 None:intergenic
TCGTACAGAAGATACCGAGC+AGG 0.572788 8.1:+33563364 MS.gene82964:CDS
AGGCCTCGATCATAAACCAC+AGG 0.572832 8.1:-33562716 None:intergenic
TCTTCACTGCAGAAAGCCAC+AGG 0.573575 8.1:+33562915 MS.gene82964:CDS
CCATACAGGATCATCAATCA+AGG 0.575427 8.1:-33562654 None:intergenic
TATTTCATAATTACCAGTCG+TGG 0.575538 8.1:-33562751 None:intergenic
GACAAGACCCAAAATCCTCC+AGG 0.575555 8.1:-33562125 None:intergenic
CGGGCGATGAAGAATAATGA+AGG 0.575646 8.1:+33560329 None:intergenic
GCTTAGTTCCTCCTCCGGAT+GGG 0.577164 8.1:+33560995 MS.gene82964:CDS
CAAAGAGCAGAGATGAATGT+TGG 0.578527 8.1:+33561040 MS.gene82964:CDS
ACGAGCGCAGATCGCGTGGA+TGG 0.579590 8.1:+33562165 MS.gene82964:CDS
AACAGGAGACTTCTGCCATA+CGG 0.581022 8.1:-33562285 None:intergenic
TTGGTGTCCTTGCTACACAC+AGG 0.584328 8.1:+33561861 MS.gene82964:CDS
CACCGGATTCTTCGTCCTGG+AGG 0.589366 8.1:+33562110 MS.gene82964:CDS
CTTGATTGATGATCCTGTAT+GGG 0.589605 8.1:+33562655 MS.gene82964:CDS
TTATTGATGATGATTTCTTG+GGG 0.593785 8.1:-33560493 None:intergenic
CTATTGCTAAGGGTATGCGA+TGG 0.603394 8.1:+33563336 MS.gene82964:CDS
GTGGTACCGAATTCTGTCCA+TGG 0.604736 8.1:-33561938 None:intergenic
GAGAAATTCCTCTTTCCTGG+TGG 0.605663 8.1:+33561233 MS.gene82964:CDS
AACAGAAGTCCAATCAACAT+TGG 0.605779 8.1:+33561195 MS.gene82964:CDS
TATTGCTAAGGGTATGCGAT+GGG 0.605922 8.1:+33563337 MS.gene82964:CDS
AGGCTGTCATCGCATTTAGG+TGG 0.605988 8.1:-33562344 None:intergenic
GCCATTACAACTATTGCTAA+GGG 0.606001 8.1:+33563326 MS.gene82964:CDS
TCTTATCAGTCCCAAGCTCA+GGG 0.607315 8.1:-33562583 None:intergenic
GTCATCGCATTTAGGTGGAT+AGG 0.608032 8.1:-33562339 None:intergenic
ATTACCCTTGGATCAGTGCA+TGG 0.610417 8.1:-33560578 None:intergenic
ATGGAAGCCTGTGTGTAGCA+AGG 0.611575 8.1:-33561868 None:intergenic
GGTGGTGATTTACTGGATCG+TGG 0.619774 8.1:+33561755 MS.gene82964:CDS
GGAAGAAGTATGGATCCTAT+CGG 0.630329 8.1:+33560923 MS.gene82964:CDS
TCCTGTATGGGTTATGAATG+TGG 0.630835 8.1:+33562667 MS.gene82964:CDS
TGCTGTGAATACACTCCCTG+TGG 0.631567 8.1:+33562700 MS.gene82964:CDS
ACCATGCTGAATCTGGTGCT+AGG 0.638733 8.1:-33562364 None:intergenic
GCCTAGCACCAGATTCAGCA+TGG 0.641516 8.1:+33562363 MS.gene82964:CDS
CAAGAGTAGGATTGTCCCTG+TGG 0.644091 8.1:+33560364 MS.gene82964:CDS
GAAAGATGGAACTATTCGAA+CGG 0.647942 8.1:+33561322 MS.gene82964:CDS
GGAGGTACTATGTTCCCGAA+CGG 0.648294 8.1:+33561254 MS.gene82964:CDS
CGTACAGAAGATACCGAGCA+GGG 0.654224 8.1:+33563365 MS.gene82964:CDS
TATTGATGATGATTTCTTGG+GGG 0.655952 8.1:-33560492 None:intergenic
GCTAGTACTTTCAAACGTGA+CGG 0.659772 8.1:+33562518 MS.gene82964:CDS
CAAGGACACCAAGAATTGCA+GGG 0.666290 8.1:-33561850 None:intergenic
GGCCATTGATACTGGTTGTG+GGG 0.668748 8.1:+33561343 MS.gene82964:CDS
TTCCAACATTATGAACCGAT+AGG 0.674489 8.1:-33560938 None:intergenic
GATATGAATACGGAATATGG+AGG 0.676561 8.1:+33562620 MS.gene82964:CDS
TGAAGGAGGTCATATGTCCG+AGG 0.677170 8.1:-33562881 None:intergenic
CAATTTCTAAGTGGCCCGAG+CGG 0.677610 8.1:+33562450 MS.gene82964:CDS
AGTCGTGGAACGTTCCAATG+AGG 0.677842 8.1:-33562736 None:intergenic
GGAAGCTGGTACCCATCCGG+AGG 0.678508 8.1:-33561006 None:intergenic
AGAGTCCTGCAAGATGAAGG+AGG 0.687154 8.1:-33562895 None:intergenic
GGCCTCGATCATAAACCACA+GGG 0.703174 8.1:-33562715 None:intergenic
GGGAACATAGTACCTCCACC+AGG 0.712101 8.1:-33561248 None:intergenic
CCACAAATATGATTGCCACA+GGG 0.719216 8.1:-33560379 None:intergenic
ACAGAAGATACCGAGCAGGG+AGG 0.757470 8.1:+33563368 MS.gene82964:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTGGTTTAAATATATTTT+TGG + chr8.1:33562980-33562999 MS.gene82964:intron 10.0%
!! AATACTGTTATTAAAACAAA+TGG + chr8.1:33562770-33562789 MS.gene82964:intron 15.0%
!!! AAATTTGTTGATATTGAAAT+TGG + chr8.1:33563062-33563081 MS.gene82964:intron 15.0%
!!! CTGATATTAATTGATTTTTT+TGG - chr8.1:33561652-33561671 None:intergenic 15.0%
!!! GTTACATTTTAATATCTTAT+GGG + chr8.1:33560612-33560631 MS.gene82964:intron 15.0%
!!! TGTTACATTTTAATATCTTA+TGG + chr8.1:33560611-33560630 MS.gene82964:intron 15.0%
!! CCAAGATAATTTATAAAAAC+AGG + chr8.1:33560760-33560779 MS.gene82964:intron 20.0%
!! GTACAAATAACAAAATTAGT+TGG - chr8.1:33561630-33561649 None:intergenic 20.0%
!! TAAAGACTAAAATGATTACA+AGG + chr8.1:33563129-33563148 MS.gene82964:intron 20.0%
!! TTAATATTCTTGAAATACAC+CGG + chr8.1:33562093-33562112 MS.gene82964:CDS 20.0%
!! TTATTGTAACAATCATTAAC+AGG - chr8.1:33562305-33562324 None:intergenic 20.0%
!!! CCTGTTTTTATAAATTATCT+TGG - chr8.1:33560763-33560782 None:intergenic 20.0%
!!! GATTTGAATTTTGTTATGAT+AGG + chr8.1:33562837-33562856 MS.gene82964:intron 20.0%
!!! GTATTTGTACACATTAATTT+TGG + chr8.1:33562963-33562982 MS.gene82964:intron 20.0%
!!! TATAGTTAATTTTACAGAGA+TGG + chr8.1:33560902-33560921 MS.gene82964:intron 20.0%
!!! TATCAGTGTTTTTTTTATCA+CGG + chr8.1:33561391-33561410 MS.gene82964:intron 20.0%
! ATAAGTTGTTTATCCAATCA+GGG + chr8.1:33560873-33560892 MS.gene82964:intron 25.0%
! CTGTTAAATGTTGCATAAAA+AGG - chr8.1:33562000-33562019 None:intergenic 25.0%
! GATCCAAAGTTTAAGAAAAT+AGG + chr8.1:33562419-33562438 MS.gene82964:CDS 25.0%
! TAATATGCAAGAAAAAGCTA+TGG + chr8.1:33563408-33563427 MS.gene82964:CDS 25.0%
! TATAAGTTGTTTATCCAATC+AGG + chr8.1:33560872-33560891 MS.gene82964:intron 25.0%
! TGATAAGATAAGAAATGTGA+TGG + chr8.1:33562598-33562617 MS.gene82964:CDS 25.0%
! TTCATAGTTACTTGTATTTG+CGG + chr8.1:33563178-33563197 MS.gene82964:intron 25.0%
!! AAAGAGAATTGTAATTTGCA+AGG - chr8.1:33561524-33561543 None:intergenic 25.0%
!! AGACCTATTTTCTTAAACTT+TGG - chr8.1:33562425-33562444 None:intergenic 25.0%
!! ATTTTCTTAAACTTTGGATC+CGG - chr8.1:33562419-33562438 None:intergenic 25.0%
!! CAATATCAACAAATTTTGCA+GGG - chr8.1:33563058-33563077 None:intergenic 25.0%
!! TCAATATCAACAAATTTTGC+AGG - chr8.1:33563059-33563078 None:intergenic 25.0%
!!! AAATGTGATGGATATGAATA+CGG + chr8.1:33562610-33562629 MS.gene82964:CDS 25.0%
!!! ACTTATTGATGATGATTTCT+TGG - chr8.1:33560498-33560517 None:intergenic 25.0%
!!! ATTTTAGTCTTTACTTTGAG+GGG - chr8.1:33563122-33563141 None:intergenic 25.0%
!!! CATTTTAGTCTTTACTTTGA+GGG - chr8.1:33563123-33563142 None:intergenic 25.0%
!!! CGGTAAATATGTTGATTTAA+TGG + chr8.1:33561280-33561299 MS.gene82964:CDS 25.0%
!!! CTTATTGATGATGATTTCTT+GGG - chr8.1:33560497-33560516 None:intergenic 25.0%
!!! TCATTTTAGTCTTTACTTTG+AGG - chr8.1:33563124-33563143 None:intergenic 25.0%
!!! TTATTGATGATGATTTCTTG+GGG - chr8.1:33560496-33560515 None:intergenic 25.0%
AAACTATTTGAGAGAGCTTA+TGG + chr8.1:33560684-33560703 MS.gene82964:intron 30.0%
AAATTATCTTGGAGAGCTTA+TGG - chr8.1:33560752-33560771 None:intergenic 30.0%
AACACTGATAAAAACGCAAA+AGG - chr8.1:33561381-33561400 None:intergenic 30.0%
AAGAGCAAAATTCATAGAAG+TGG - chr8.1:33561960-33561979 None:intergenic 30.0%
AGATATAACAGCAAACATGA+CGG - chr8.1:33561141-33561160 None:intergenic 30.0%
ATAGTTCCATCTTTCATTTC+TGG - chr8.1:33561317-33561336 None:intergenic 30.0%
ATGGATATGAATACGGAATA+TGG + chr8.1:33562617-33562636 MS.gene82964:CDS 30.0%
ATGTGGACTTTCTTTCTATA+TGG + chr8.1:33560406-33560425 MS.gene82964:CDS 30.0%
CAGGAAACTTAAAATTATGC+CGG - chr8.1:33561109-33561128 None:intergenic 30.0%
CCATATCGAAAGAATTTGAT+GGG - chr8.1:33561895-33561914 None:intergenic 30.0%
GAAGAATAATGAAGGAAAAG+TGG + chr8.1:33560337-33560356 MS.gene82964:CDS 30.0%
GACGAATGATAATATAAGAC+CGG + chr8.1:33561087-33561106 MS.gene82964:intron 30.0%
GAGCTAGAATATGATTGTTA+AGG - chr8.1:33561547-33561566 None:intergenic 30.0%
GTCTTACATCAATTTCTAAG+TGG + chr8.1:33562441-33562460 MS.gene82964:CDS 30.0%
TAGTTCCATCTTTCATTTCT+GGG - chr8.1:33561316-33561335 None:intergenic 30.0%
TATTTCATAATTACCAGTCG+TGG - chr8.1:33562754-33562773 None:intergenic 30.0%
TGAAATGAAGTACGTATTGT+TGG + chr8.1:33563238-33563257 MS.gene82964:CDS 30.0%
TGATACAATTCTATCGTTCT+CGG - chr8.1:33560447-33560466 None:intergenic 30.0%
TGTGGACTTTCTTTCTATAT+GGG + chr8.1:33560407-33560426 MS.gene82964:CDS 30.0%
TTAATGATCATGTGTCTGTT+TGG + chr8.1:33560655-33560674 MS.gene82964:intron 30.0%
TTCTATATGGGTGTAATCTT+TGG + chr8.1:33560419-33560438 MS.gene82964:CDS 30.0%
TTCTGACAATGATCGTTATT+TGG + chr8.1:33563209-33563228 MS.gene82964:intron 30.0%
! AATATTGCACGTTTTGCTTT+TGG + chr8.1:33563011-33563030 MS.gene82964:intron 30.0%
! ACAAAACTCTTGCTCATTTT+AGG + chr8.1:33561721-33561740 MS.gene82964:intron 30.0%
! CAATCAACATTGGTTGAAAA+AGG + chr8.1:33561205-33561224 MS.gene82964:CDS 30.0%
! CATTCTTTTCTCTCGAAAAT+TGG - chr8.1:33560976-33560995 None:intergenic 30.0%
! TAAAATGAGCAAGAGTTTTG+TGG - chr8.1:33561722-33561741 None:intergenic 30.0%
! TGTTTGTTAATCCAGTCATA+TGG - chr8.1:33561179-33561198 None:intergenic 30.0%
!! TATTGATGATGATTTCTTGG+GGG - chr8.1:33560495-33560514 None:intergenic 30.0%
!!! CTTTTTCAACCAATGTTGAT+TGG - chr8.1:33561207-33561226 None:intergenic 30.0%
AACAGAAGTCCAATCAACAT+TGG + chr8.1:33561195-33561214 MS.gene82964:CDS 35.0%
AACTATACTTAGACCCTGAT+TGG - chr8.1:33560889-33560908 None:intergenic 35.0%
ACCCTTAGCAATAGTTGTAA+TGG - chr8.1:33563330-33563349 None:intergenic 35.0%
CCCATCAAATTCTTTCGATA+TGG + chr8.1:33561892-33561911 MS.gene82964:CDS 35.0%
CTTATTCCATGGACAGAATT+CGG + chr8.1:33561932-33561951 MS.gene82964:CDS 35.0%
GAAAGATGGAACTATTCGAA+CGG + chr8.1:33561322-33561341 MS.gene82964:CDS 35.0%
GAAGTACGTATTGTTGGAAA+TGG + chr8.1:33563244-33563263 MS.gene82964:CDS 35.0%
GATATGAATACGGAATATGG+AGG + chr8.1:33562620-33562639 MS.gene82964:CDS 35.0%
GCCATATCGAAAGAATTTGA+TGG - chr8.1:33561896-33561915 None:intergenic 35.0%
GCCATTACAACTATTGCTAA+GGG + chr8.1:33563326-33563345 MS.gene82964:CDS 35.0%
TCAGAAATGTTCCATATGAC+TGG + chr8.1:33561165-33561184 MS.gene82964:intron 35.0%
TCGAAAGAATTTGATGGGAA+TGG - chr8.1:33561890-33561909 None:intergenic 35.0%
TGATAGTCAATACTGCATTC+AGG - chr8.1:33560548-33560567 None:intergenic 35.0%
TGCCATTACAACTATTGCTA+AGG + chr8.1:33563325-33563344 MS.gene82964:CDS 35.0%
TTACAGAGATGGAAGAAGTA+TGG + chr8.1:33560913-33560932 MS.gene82964:intron 35.0%
TTCCAACATTATGAACCGAT+AGG - chr8.1:33560941-33560960 None:intergenic 35.0%
! CAACATTGGTTGAAAAAGGA+AGG + chr8.1:33561209-33561228 MS.gene82964:CDS 35.0%
! CTTGATTGATGATCCTGTAT+GGG + chr8.1:33562655-33562674 MS.gene82964:CDS 35.0%
! CTTTTCTCTCGAAAATTGGA+GGG - chr8.1:33560972-33560991 None:intergenic 35.0%
! GGGACTAGTATAAAACAACA+AGG - chr8.1:33563038-33563057 None:intergenic 35.0%
! TCTTTTCTCTCGAAAATTGG+AGG - chr8.1:33560973-33560992 None:intergenic 35.0%
!! ATCATATTTGTGGTGCTATG+TGG + chr8.1:33560389-33560408 MS.gene82964:CDS 35.0%
!! ATCCTATCGGTTCATAATGT+TGG + chr8.1:33560936-33560955 MS.gene82964:CDS 35.0%
!!! AAGCTTTTTCGACATATCCT+CGG + chr8.1:33562864-33562883 MS.gene82964:CDS 35.0%
!!! ACAGCACTGGTTTTTATGTA+GGG + chr8.1:33561493-33561512 MS.gene82964:intron 35.0%
!!! GTTTTTTTTATCACGGAGCA+CGG + chr8.1:33561398-33561417 MS.gene82964:intron 35.0%
ACATATTTACCGACACCGTT+CGG - chr8.1:33561272-33561291 None:intergenic 40.0%
ACCACAAATATGATTGCCAC+AGG - chr8.1:33560383-33560402 None:intergenic 40.0%
ACCACATTCATAACCCATAC+AGG - chr8.1:33562671-33562690 None:intergenic 40.0%
ACGACAAAACAAGTGCGAAT+TGG - chr8.1:33562395-33562414 None:intergenic 40.0%
ACTGCATTCAGGAAAACTGA+TGG - chr8.1:33560537-33560556 None:intergenic 40.0%
ACTGGATCGTGGAATTCTTA+CGG + chr8.1:33561766-33561785 MS.gene82964:CDS 40.0%
ATGATTTCTTGGGGGAATCA+AGG - chr8.1:33560487-33560506 None:intergenic 40.0%
CAAAGAGCAGAGATGAATGT+TGG + chr8.1:33561040-33561059 MS.gene82964:CDS 40.0%
CAACATTCATCTCTGCTCTT+TGG - chr8.1:33561042-33561061 None:intergenic 40.0%
CACTGCACTAATATTACCCT+TGG - chr8.1:33560593-33560612 None:intergenic 40.0%
CATTAGAATGAGCTGCTTGT+TGG + chr8.1:33562941-33562960 MS.gene82964:intron 40.0%
CCACAAATATGATTGCCACA+GGG - chr8.1:33560382-33560401 None:intergenic 40.0%
CCATACAGGATCATCAATCA+AGG - chr8.1:33562657-33562676 None:intergenic 40.0%
CGAATTCTGTCCATGGAATA+AGG - chr8.1:33561934-33561953 None:intergenic 40.0%
CTGATCCCAGAAATGAAAGA+TGG + chr8.1:33561308-33561327 MS.gene82964:CDS 40.0%
GAAAAGTGGTAGACAAGAGT+AGG + chr8.1:33560351-33560370 MS.gene82964:CDS 40.0%
GAATCTCAATGGTTCGTCAT+GGG + chr8.1:33562492-33562511 MS.gene82964:CDS 40.0%
GCTAGTACTTTCAAACGTGA+CGG + chr8.1:33562518-33562537 MS.gene82964:CDS 40.0%
TAACTACTATTCGACAGCAC+TGG + chr8.1:33561480-33561499 MS.gene82964:intron 40.0%
TACACCAGATCGAATCTCAA+TGG + chr8.1:33562481-33562500 MS.gene82964:CDS 40.0%
TCAAGAATATTAACTCCGCC+TGG - chr8.1:33562086-33562105 None:intergenic 40.0%
TCTCGCGATAGACAAAAGAA+TGG + chr8.1:33561685-33561704 MS.gene82964:intron 40.0%
TGATTTCTTGGGGGAATCAA+GGG - chr8.1:33560486-33560505 None:intergenic 40.0%
! CCTTGATTGATGATCCTGTA+TGG + chr8.1:33562654-33562673 MS.gene82964:CDS 40.0%
! GGAAGAAGTATGGATCCTAT+CGG + chr8.1:33560923-33560942 MS.gene82964:CDS 40.0%
! TATTGCTAAGGGTATGCGAT+GGG + chr8.1:33563337-33563356 MS.gene82964:CDS 40.0%
! TCACACTGCAATCTTTTACC+AGG + chr8.1:33562065-33562084 MS.gene82964:intron 40.0%
! TCCTGTATGGGTTATGAATG+TGG + chr8.1:33562667-33562686 MS.gene82964:CDS 40.0%
!!! CTCATTTTAGGTTGCTAGCT+GGG + chr8.1:33561733-33561752 MS.gene82964:intron 40.0%
!!! GACAGCACTGGTTTTTATGT+AGG + chr8.1:33561492-33561511 MS.gene82964:intron 40.0%
!!! TCATTTTAGGTTGCTAGCTG+GGG + chr8.1:33561734-33561753 MS.gene82964:intron 40.0%
AAAAAGGCGACATTGCCGTA+TGG + chr8.1:33562270-33562289 MS.gene82964:CDS 45.0%
AAATTCCTCTTTCCTGGTGG+AGG + chr8.1:33561236-33561255 MS.gene82964:CDS 45.0%
AACAGGAGACTTCTGCCATA+CGG - chr8.1:33562288-33562307 None:intergenic 45.0%
AAGAGATAACCACCAAGCTC+AGG + chr8.1:33561802-33561821 MS.gene82964:CDS 45.0%
AAGCGAATTGAACCTGAGCT+TGG - chr8.1:33561817-33561836 None:intergenic 45.0%
AGAATGCTTAGTTCCTCCTC+CGG + chr8.1:33560990-33561009 MS.gene82964:CDS 45.0%
AGCCACCAGGTCGTAAAATT+CGG - chr8.1:33563271-33563290 None:intergenic 45.0%
AGCTCAGGTTCAATTCGCTT+TGG + chr8.1:33561817-33561836 MS.gene82964:CDS 45.0%
ATCTTATCAGTCCCAAGCTC+AGG - chr8.1:33562587-33562606 None:intergenic 45.0%
ATTACCCTTGGATCAGTGCA+TGG - chr8.1:33560581-33560600 None:intergenic 45.0%
ATTCGAACGGCCATTGATAC+TGG + chr8.1:33561335-33561354 MS.gene82964:CDS 45.0%
CAAGGACACCAAGAATTGCA+GGG - chr8.1:33561853-33561872 None:intergenic 45.0%
CATATTTACCGACACCGTTC+GGG - chr8.1:33561271-33561290 None:intergenic 45.0%
CCCTGTGGCAATCATATTTG+TGG + chr8.1:33560379-33560398 MS.gene82964:CDS 45.0%
CGAACCATTGAGATTCGATC+TGG - chr8.1:33562488-33562507 None:intergenic 45.0%
CGAATCTCAATGGTTCGTCA+TGG + chr8.1:33562491-33562510 MS.gene82964:CDS 45.0%
CTGCTCTTTGGCCATCTAAT+TGG - chr8.1:33561030-33561049 None:intergenic 45.0%
GAGAAATTCCTCTTTCCTGG+TGG + chr8.1:33561233-33561252 MS.gene82964:CDS 45.0%
GCAGAGATGAATGTTGGTAC+AGG + chr8.1:33561046-33561065 MS.gene82964:CDS 45.0%
GCTCATTCTAATGTCACCTG+TGG - chr8.1:33562934-33562953 None:intergenic 45.0%
GGAGAGAAATTCCTCTTTCC+TGG + chr8.1:33561230-33561249 MS.gene82964:CDS 45.0%
GGAGTATACCATGCTGAATC+TGG - chr8.1:33562374-33562393 None:intergenic 45.0%
GGTACCAGCTTCCAATTAGA+TGG + chr8.1:33561016-33561035 MS.gene82964:CDS 45.0%
GTCATCGCATTTAGGTGGAT+AGG - chr8.1:33562342-33562361 None:intergenic 45.0%
GTTTATGATCGAGGCCTCAT+TGG + chr8.1:33562722-33562741 MS.gene82964:CDS 45.0%
TATGACCTCCTTCATCTTGC+AGG + chr8.1:33562890-33562909 MS.gene82964:CDS 45.0%
TCAAACGTGACGGCAGTAAT+TGG + chr8.1:33562528-33562547 MS.gene82964:CDS 45.0%
TCTCGAAAATTGGAGGGCAA+TGG - chr8.1:33560966-33560985 None:intergenic 45.0%
TCTTATCAGTCCCAAGCTCA+GGG - chr8.1:33562586-33562605 None:intergenic 45.0%
TGAAGAGTCCTGCAAGATGA+AGG - chr8.1:33562901-33562920 None:intergenic 45.0%
TTGGCCATCTAATTGGAAGC+TGG - chr8.1:33561023-33561042 None:intergenic 45.0%
! AACATGACGGAGTAAGTTGC+AGG - chr8.1:33561128-33561147 None:intergenic 45.0%
! AAGAAGTTGATCCCTGAGCT+TGG + chr8.1:33562572-33562591 MS.gene82964:CDS 45.0%
! AGAAGTTGATCCCTGAGCTT+GGG + chr8.1:33562573-33562592 MS.gene82964:CDS 45.0%
! ATGGACCGAATTTTACGACC+TGG + chr8.1:33563263-33563282 MS.gene82964:CDS 45.0%
! CACTGCAATCTTTTACCAGG+CGG + chr8.1:33562068-33562087 MS.gene82964:intron 45.0%
! CTATTGCTAAGGGTATGCGA+TGG + chr8.1:33563336-33563355 MS.gene82964:CDS 45.0%
! GGAATATGGAGGTTTTGCTG+CGG + chr8.1:33562631-33562650 MS.gene82964:CDS 45.0%
! TCTTCGTCCTGGAGGATTTT+GGG + chr8.1:33562118-33562137 MS.gene82964:CDS 45.0%
! TGAGCTTGGTGGTTATCTCT+TGG - chr8.1:33561803-33561822 None:intergenic 45.0%
! TGTCCATGGAATAAGGCATC+TGG - chr8.1:33561927-33561946 None:intergenic 45.0%
! TTCTTCGTCCTGGAGGATTT+TGG + chr8.1:33562117-33562136 MS.gene82964:CDS 45.0%
!!! GCGCGAGTCTAGTTACTTTT+TGG + chr8.1:33563301-33563320 MS.gene82964:CDS 45.0%
!!! GCTCATTTTAGGTTGCTAGC+TGG + chr8.1:33561732-33561751 MS.gene82964:intron 45.0%
!!! TCGCACTTGTTTTGTCGTTC+CGG + chr8.1:33562397-33562416 MS.gene82964:CDS 45.0%
!! TTTAAATTGTATAATAAAAA+AGG + chr8.1:33562254-33562273 MS.gene82964:CDS 5.0%
ACCATGCTGAATCTGGTGCT+AGG - chr8.1:33562367-33562386 None:intergenic 50.0%
ACTATGTTCCCGAACGGTGT+CGG + chr8.1:33561260-33561279 MS.gene82964:CDS 50.0%
AGAGTCCTGCAAGATGAAGG+AGG - chr8.1:33562898-33562917 None:intergenic 50.0%
AGGCCTCGATCATAAACCAC+AGG - chr8.1:33562719-33562738 None:intergenic 50.0%
AGGCTGTCATCGCATTTAGG+TGG - chr8.1:33562347-33562366 None:intergenic 50.0%
AGTCGTGGAACGTTCCAATG+AGG - chr8.1:33562739-33562758 None:intergenic 50.0%
ATACACCGGATTCTTCGTCC+TGG + chr8.1:33562107-33562126 MS.gene82964:CDS 50.0%
ATCCTCCAGGACGAAGAATC+CGG - chr8.1:33562115-33562134 None:intergenic 50.0%
ATGGAAGCCTGTGTGTAGCA+AGG - chr8.1:33561871-33561890 None:intergenic 50.0%
ATTGGAAGCTGGTACCCATC+CGG - chr8.1:33561012-33561031 None:intergenic 50.0%
CAAGAGTAGGATTGTCCCTG+TGG + chr8.1:33560364-33560383 MS.gene82964:CDS 50.0%
CAATTTCTAAGTGGCCCGAG+CGG + chr8.1:33562450-33562469 MS.gene82964:CDS 50.0%
CACCCCACAACCAGTATCAA+TGG - chr8.1:33561348-33561367 None:intergenic 50.0%
CGAATTGAACCTGAGCTTGG+TGG - chr8.1:33561814-33561833 None:intergenic 50.0%
CGTACAGAAGATACCGAGCA+GGG + chr8.1:33563365-33563384 MS.gene82964:CDS 50.0%
CGTGGTATCCCTGCAATTCT+TGG + chr8.1:33561842-33561861 MS.gene82964:CDS 50.0%
CTCCCTGTGGTTTATGATCG+AGG + chr8.1:33562713-33562732 MS.gene82964:CDS 50.0%
GACAAGACCCAAAATCCTCC+AGG - chr8.1:33562128-33562147 None:intergenic 50.0%
GATCTGCGCTCGTAGTTGAT+TGG - chr8.1:33562158-33562177 None:intergenic 50.0%
GCAAGGACACCAAGAATTGC+AGG - chr8.1:33561854-33561873 None:intergenic 50.0%
GCTAGGCTGTCATCGCATTT+AGG - chr8.1:33562350-33562369 None:intergenic 50.0%
GGCCTCGATCATAAACCACA+GGG - chr8.1:33562718-33562737 None:intergenic 50.0%
GGTGGTGATTTACTGGATCG+TGG + chr8.1:33561755-33561774 MS.gene82964:CDS 50.0%
TAGCTGGGGTGGTGATTTAC+TGG + chr8.1:33561748-33561767 MS.gene82964:CDS 50.0%
TAGTACCTCCACCAGGAAAG+AGG - chr8.1:33561244-33561263 None:intergenic 50.0%
TCACGGAGCACGGAAAATAG+CGG + chr8.1:33561408-33561427 MS.gene82964:intron 50.0%
TCATTGGAACGTTCCACGAC+TGG + chr8.1:33562738-33562757 MS.gene82964:CDS 50.0%
TCGTACAGAAGATACCGAGC+AGG + chr8.1:33563364-33563383 MS.gene82964:CDS 50.0%
TCTTCACTGCAGAAAGCCAC+AGG + chr8.1:33562915-33562934 MS.gene82964:CDS 50.0%
TGAAGGAGGTCATATGTCCG+AGG - chr8.1:33562884-33562903 None:intergenic 50.0%
TGCTGTGAATACACTCCCTG+TGG + chr8.1:33562700-33562719 MS.gene82964:CDS 50.0%
TGGTGTAACATGCAACCGCT+CGG - chr8.1:33562468-33562487 None:intergenic 50.0%
! ACACTCCATGCACTGATCCA+AGG + chr8.1:33560573-33560592 MS.gene82964:CDS 50.0%
! ACGGCCATTGATACTGGTTG+TGG + chr8.1:33561341-33561360 MS.gene82964:CDS 50.0%
! CACTCCATGCACTGATCCAA+GGG + chr8.1:33560574-33560593 MS.gene82964:CDS 50.0%
! CGGCCATTGATACTGGTTGT+GGG + chr8.1:33561342-33561361 MS.gene82964:CDS 50.0%
! CTCGTAGTTGATTGGTGGAC+CGG - chr8.1:33562150-33562169 None:intergenic 50.0%
! GACCGAATTTTACGACCTGG+TGG + chr8.1:33563266-33563285 MS.gene82964:CDS 50.0%
! GCTCCAGATGCCTTATTCCA+TGG + chr8.1:33561921-33561940 MS.gene82964:CDS 50.0%
! GGCCATTGATACTGGTTGTG+GGG + chr8.1:33561343-33561362 MS.gene82964:CDS 50.0%
! GTGGTACCGAATTCTGTCCA+TGG - chr8.1:33561941-33561960 None:intergenic 50.0%
! TTGGTGTCCTTGCTACACAC+AGG + chr8.1:33561861-33561880 MS.gene82964:CDS 50.0%
!! GGAGGATTTTGGGTCTTGTC+CGG + chr8.1:33562128-33562147 MS.gene82964:CDS 50.0%
!! GGAGGTACTATGTTCCCGAA+CGG + chr8.1:33561254-33561273 MS.gene82964:CDS 50.0%
!! GTTCAATTCGCTTTGGAGCG+TGG + chr8.1:33561824-33561843 MS.gene82964:CDS 50.0%
!!! TTTTAGGTTGCTAGCTGGGG+TGG + chr8.1:33561737-33561756 MS.gene82964:intron 50.0%
ACAGAAGATACCGAGCAGGG+AGG + chr8.1:33563368-33563387 MS.gene82964:CDS 55.0%
CTGCGCTCGTAGTTGATTGG+TGG - chr8.1:33562155-33562174 None:intergenic 55.0%
GCCTAGCACCAGATTCAGCA+TGG + chr8.1:33562363-33562382 MS.gene82964:CDS 55.0%
GCTTAGTTCCTCCTCCGGAT+GGG + chr8.1:33560995-33561014 MS.gene82964:CDS 55.0%
GGGAACATAGTACCTCCACC+AGG - chr8.1:33561251-33561270 None:intergenic 55.0%
GGTGTAACATGCAACCGCTC+GGG - chr8.1:33562467-33562486 None:intergenic 55.0%
TGCTTAGTTCCTCCTCCGGA+TGG + chr8.1:33560994-33561013 MS.gene82964:CDS 55.0%
TGGGGGAATCAAGGGATCTC+TGG - chr8.1:33560478-33560497 None:intergenic 55.0%
AACTACGAGCGCAGATCGCG+TGG + chr8.1:33562161-33562180 MS.gene82964:CDS 60.0%
CACCGGATTCTTCGTCCTGG+AGG + chr8.1:33562110-33562129 MS.gene82964:CDS 60.0%
CTCCATGTCACCTCCCTGCT+CGG - chr8.1:33563381-33563400 None:intergenic 60.0%
TACCGAGCAGGGAGGTGACA+TGG + chr8.1:33563376-33563395 MS.gene82964:CDS 60.0%
ACGAGCGCAGATCGCGTGGA+TGG + chr8.1:33562165-33562184 MS.gene82964:CDS 65.0%
AGCTGGTACCCATCCGGAGG+AGG - chr8.1:33561006-33561025 None:intergenic 65.0%
GGAAGCTGGTACCCATCCGG+AGG - chr8.1:33561009-33561028 None:intergenic 65.0%
! CGCACGAGAGCATAGCCACC+AGG - chr8.1:33563284-33563303 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 33560335 33563457 33560335 ID=MS.gene82964
chr8.1 mRNA 33560335 33563457 33560335 ID=MS.gene82964.t1;Parent=MS.gene82964
chr8.1 exon 33560335 33560595 33560335 ID=MS.gene82964.t1.exon1;Parent=MS.gene82964.t1
chr8.1 CDS 33560335 33560595 33560335 ID=cds.MS.gene82964.t1;Parent=MS.gene82964.t1
chr8.1 exon 33560919 33561067 33560919 ID=MS.gene82964.t1.exon2;Parent=MS.gene82964.t1
chr8.1 CDS 33560919 33561067 33560919 ID=cds.MS.gene82964.t1;Parent=MS.gene82964.t1
chr8.1 exon 33561169 33561364 33561169 ID=MS.gene82964.t1.exon3;Parent=MS.gene82964.t1
chr8.1 CDS 33561169 33561364 33561169 ID=cds.MS.gene82964.t1;Parent=MS.gene82964.t1
chr8.1 exon 33561743 33561953 33561743 ID=MS.gene82964.t1.exon4;Parent=MS.gene82964.t1
chr8.1 CDS 33561743 33561953 33561743 ID=cds.MS.gene82964.t1;Parent=MS.gene82964.t1
chr8.1 exon 33562087 33562759 33562087 ID=MS.gene82964.t1.exon5;Parent=MS.gene82964.t1
chr8.1 CDS 33562087 33562759 33562087 ID=cds.MS.gene82964.t1;Parent=MS.gene82964.t1
chr8.1 exon 33562859 33562936 33562859 ID=MS.gene82964.t1.exon6;Parent=MS.gene82964.t1
chr8.1 CDS 33562859 33562936 33562859 ID=cds.MS.gene82964.t1;Parent=MS.gene82964.t1
chr8.1 exon 33563235 33563457 33563235 ID=MS.gene82964.t1.exon7;Parent=MS.gene82964.t1
chr8.1 CDS 33563235 33563457 33563235 ID=cds.MS.gene82964.t1;Parent=MS.gene82964.t1
Gene Sequence

>MS.gene82964

ATGAAGAATAATGAAGGAAAAGTGGTAGACAAGAGTAGGATTGTCCCTGTGGCAATCATATTTGTGGTGCTATGTGGACTTTCTTTCTATATGGGTGTAATCTTTGGTTCCGAGAACGATAGAATTGTATCAATTATCAGCCAGAGATCCCTTGATTCCCCCAAGAAATCATCATCAATAAGTTCTTTGCAAATTAAATCCATCAGTTTTCCTGAATGCAGTATTGACTATCAAGATTACACTCCATGCACTGATCCAAGGGTAATATTAGTGCAGTGTTACATTTTAATATCTTATGGGATTTGTAGAAGAAAGTTTTCTTAATGATCATGTGTCTGTTTGGATCGTAAAACTATTTGAGAGAGCTTATGGAAACAACTTATGATATGTCTATAAGTTGTTTTCAGTTTATTTCCATAAGCTCTCCAAGATAATTTATAAAAACAGGCTATATCTTGTATGAAAACAATTTGTCTTTATTTTATCTTTTATTATAGATATAGCTTATTCACAATCATTTACAATAAGCTCTTATGCTATAAGTTGTTTATCCAATCAGGGTCTAAGTATAGTTAATTTTACAGAGATGGAAGAAGTATGGATCCTATCGGTTCATAATGTTGGAACGCCATTGCCCTCCAATTTTCGAGAGAAAAGAATGCTTAGTTCCTCCTCCGGATGGGTACCAGCTTCCAATTAGATGGCCAAAGAGCAGAGATGAATGTTGGTACAGGTAATTATATCAGTCTGACGACGAATGATAATATAAGACCGGCATAATTTTAAGTTTCCTGCAACTTACTCCGTCATGTTTGCTGTTATATCTAATTTCAGAAATGTTCCATATGACTGGATTAACAAACAGAAGTCCAATCAACATTGGTTGAAAAAGGAAGGAGAGAAATTCCTCTTTCCTGGTGGAGGTACTATGTTCCCGAACGGTGTCGGTAAATATGTTGATTTAATGGAAGCTCTGATCCCAGAAATGAAAGATGGAACTATTCGAACGGCCATTGATACTGGTTGTGGGGTGAGTCTTGCTTCCTTTTGCGTTTTTATCAGTGTTTTTTTTATCACGGAGCACGGAAAATAGCGGTATGCTCAAACTCTGCTACACTACAGTCTACAGCGCTATAGTATCGCTATAACTACTATTCGACAGCACTGGTTTTTATGTAGGGAATAGCCTTGCAAATTACAATTCTCTTTCCTTAACAATCATATTCTAGCTCAATTATATGTGCTTCTTCTTGCAGTTTGCTTTTCTAACTGCATTGTAATATATTTTAAAACCAACTAATTTTGTTATTTGTACCAAAAAAATCAATTAATATCAGTTGCAATTTTCAATCTCGCGATAGACAAAAGAATGGCATCAATTAATCCACAAAACTCTTGCTCATTTTAGGTTGCTAGCTGGGGTGGTGATTTACTGGATCGTGGAATTCTTACGGTTTCTCTTGCTCCAAGAGATAACCACCAAGCTCAGGTTCAATTCGCTTTGGAGCGTGGTATCCCTGCAATTCTTGGTGTCCTTGCTACACACAGGCTTCCATTCCCATCAAATTCTTTCGATATGGCTCATTGCTCCAGATGCCTTATTCCATGGACAGAATTCGGTACCACTTCTATGAATTTTGCTCTTAATCAAACTGAATTAATCCTTTTTATGCAACATTTAACAGTGATCATGAACTTTACAAGTCTTGTAGCTTCTTAAACTTTGAAATTCACACTGCAATCTTTTACCAGGCGGAGTTAATATTCTTGAAATACACCGGATTCTTCGTCCTGGAGGATTTTGGGTCTTGTCCGGTCCACCAATCAACTACGAGCGCAGATCGCGTGGATGGAACACAACTATCGAAGAGCAAAGATCAACTTACGAGAAATTGCAGAACTTACTGACTTCATTGTGCTTTAAATTGTATAATAAAAAAGGCGACATTGCCGTATGGCAGAAGTCTCCTGTTAATGATTGTTACAATAAGCTGACTAGAGATACCTATCCACCTAAATGCGATGACAGCCTAGCACCAGATTCAGCATGGTATACTCCAATTCGCACTTGTTTTGTCGTTCCGGATCCAAAGTTTAAGAAAATAGGTCTTACATCAATTTCTAAGTGGCCCGAGCGGTTGCATGTTACACCAGATCGAATCTCAATGGTTCGTCATGGGAGTGCTAGTACTTTCAAACGTGACGGCAGTAATTGGAAGAAGCATGTCGCACACTACAAGAAGTTGATCCCTGAGCTTGGGACTGATAAGATAAGAAATGTGATGGATATGAATACGGAATATGGAGGTTTTGCTGCGGCCTTGATTGATGATCCTGTATGGGTTATGAATGTGGTCTCATCTTATGCTGTGAATACACTCCCTGTGGTTTATGATCGAGGCCTCATTGGAACGTTCCACGACTGGTAATTATGAAATACTGTTATTAAAACAAATGGTTTCATTTCTGTATATAATTTTGTCTTATAAATGATCTACTGTTGATTTGAATTTTGTTATGATAGGTGTGAAGCTTTTTCGACATATCCTCGGACATATGACCTCCTTCATCTTGCAGGACTCTTCACTGCAGAAAGCCACAGGTGACATTAGAATGAGCTGCTTGTTGGTATTTGTACACATTAATTTTGGTTTAAATATATTTTTGGTCTTTGCAAATATTGCACGTTTTGCTTTTGGTCCTTGTTGTTTTATACTAGTCCCTGCAAAATTTGTTGATATTGAAATTGGTGTTTGTAACATGTAAAATGTATTTGATTTTAGTCCCCTCAAAGTAAAGACTAAAATGATTACAAGGCTAATTTGATAAGTGATGATACTTGTTTCATAGTTACTTGTATTTGCGGCTGCTTGATTCTGACAATGATCGTTATTTGGCAGATGTGAAATGAAGTACGTATTGTTGGAAATGGACCGAATTTTACGACCTGGTGGCTATGCTCTCGTGCGCGAGTCTAGTTACTTTTTGGATGCCATTACAACTATTGCTAAGGGTATGCGATGGGAATGTCGTACAGAAGATACCGAGCAGGGAGGTGACATGGAGAAGATACTAATATGCAAGAAAAAGCTATGGTATTCATCAAATAAAGATTCAAGATGA
Protein sequence

>MS.gene82964.t1

MKNNEGKVVDKSRIVPVAIIFVVLCGLSFYMGVIFGSENDRIVSIISQRSLDSPKKSSSISSLQIKSISFPECSIDYQDYTPCTDPRRWKKYGSYRFIMLERHCPPIFERKECLVPPPDGYQLPIRWPKSRDECWYRNVPYDWINKQKSNQHWLKKEGEKFLFPGGGTMFPNGVGKYVDLMEALIPEMKDGTIRTAIDTGCGVASWGGDLLDRGILTVSLAPRDNHQAQVQFALERGIPAILGVLATHRLPFPSNSFDMAHCSRCLIPWTEFGGVNILEIHRILRPGGFWVLSGPPINYERRSRGWNTTIEEQRSTYEKLQNLLTSLCFKLYNKKGDIAVWQKSPVNDCYNKLTRDTYPPKCDDSLAPDSAWYTPIRTCFVVPDPKFKKIGLTSISKWPERLHVTPDRISMVRHGSASTFKRDGSNWKKHVAHYKKLIPELGTDKIRNVMDMNTEYGGFAAALIDDPVWVMNVVSSYAVNTLPVVYDRGLIGTFHDWCEAFSTYPRTYDLLHLAGLFTAESHRCEMKYVLLEMDRILRPGGYALVRESSYFLDAITTIAKGMRWECRTEDTEQGGDMEKILICKKKLWYSSNKDSR